Graham, Page 1 Combating the Invasive Species of Asian Carp By Catherine Graham Edited by Nicholas Falduto (Photography by Spencer Green) Graham, Page 2 Table of Contents Abstract: 2-3 Ecosystems and Disturbances: 3-4 Invasive Species: 4-5 Lineage of Asian Carp: 5 Pathways of Introduction: 5-6 Types of Asian Carp: 6-10 Natural Predators to the Asian Carp: 10-12 Special Asian Carp Bodily Functions: 13-14 Special Adaptations of the Asian Carp: 14 Spawning Habits of the Asian Carp: 14- 17 Locating the Asian Carp Invasion: 17-18 Methods of Controlling the Asian Carp Population: 18-22 Where are they now?: 22-26 How endangered are the Great Lakes? : 26-28 Conclusion: 38-30 Works Cited: 31-34 Acknowledgements: 34 Abstract In the freshwater rivers in North America, the Asian Carp have threatened to disturb the ecosystem. There are four types of Asian Carp that are harming the environment: the Grass Carp, Black Carp, Bighead Carp, and Silver Carp. These Carp overpopulate areas, eating the natural resources causing native inhabitants to decrease in population. Graham, Page 3 Asian Carp are a risk to the Great Lakes’ ecosystem and fishing industries. There are no natural predators of adult Asian Carp living, and human intervention is necessary to suppress the Asian Carp populations. Asian Carp are tracked by eDNA testing and once located, electric fences are put up to guide the Asian Carp away. The chemical Rotenone, a harmful pesticide, is used to kill the Asian Carp and control the population. Asian Carp are a natural source of Omega 3 fatty acid and should be consumed, used as fertilizer, and harvested for Omega 3 fatty acid. ______________________________________________________________________________ Ecosystems and Disturbances “An ecosystem is the sum of all organisms living within its boundaries and all the abiotic factors with which they interact” (Alters, Sandra, Biology). Each ecosystem is special in the interdependence between animals that live there and the resources, both biotic and abiotic, that exist within that region (see figure A); A delicate balance exists. “If an ecosystem is disrupted by artificial or natural means (see figure B), it will not only affect all the living organisms within the ecosystem, but will also affect neighboring ecosystems” (Alters, Sandra, Biology). If two or more ecosystems begin to experience alterations, then this can cause a chain reaction that will leave the abiotic and biotic components permanently altered. These large scale changes within an ecosystem can have deleterious effects on our biosphere and the native inhabitants within each individual ecosystem (Alters, Sandra, Biology). Food cycles, energy webs, and ecological niches can be altered forever through ecological disturbances. Graham, Page 4 Figure A: In an ecosystem, all organisms are dependent on each other. A delicate balance exists between producers and consumers.; “Ecology”; Honors Biology @ Laurenceville; http://biologywithsaxe.blogspot.com, 12 Apr. 2010; Web; 2 Jan. 2014. Figure B: Anthropogenic disturbances are examples of disturbances that can disrupt and change an ecosystem for generations of life, or possibly forever.; “Background on Disturbances”; NASACASA Project; http://geo.arc.nasa.gov, n.d.; Web; 2 Jan. 2014. Invasive Species Graham, Page 5 “An ‘invasive species’ is defined by the Executive Order as a species that is 1) nonnative (or alien) to the ecosystem under consideration and 2) whose introduction causes or is likely to cause economic or environmental harm or harm to human health” (Invasive Species, National Invasive). Invasive species can be introduced into the environment by physical pathways; both man made and naturally made. Most invasive species are a result of human interference with the environment. Lineage of Asian Carp “Silver carp, Hypophthalmichthys molitrix, are native to eastern Asia and are commonly found in northeastern China and Siberia” (Hypophthalmichthys molitrix Carp). Asian Carp are not native to the North American freshwater ecosystems, only to Asia (hence the name). There are four types of invasive Asian Carp, “Bighead Carp (Hypophthalmichthys nobilis), Black Carp (Mylopharyngodon piceus), Grass Carp (Ctenopharyngodon idella), and Silver Carp (Hypophthalmichthys molitrix)” (Invasive Species, Asian Carp). Pathways of introduction Asian Carp were introduced to the United States in order to control weed growth in aquatic farms, however the carp were not carefully kept and introduced to the American ecosystems during periods of flooding. “Bighead and Silver carp were first imported into the United States in the early 1970s. Soon after, both species were being used in research projects and were stocked into wastewater treatment lagoons and aquaculture ponds in several states without regard to their potential effects on the ecosystems to which they were introduced or on the species inhabiting them. Bighead and Silver carp escaped confinement during flood events and are now well established with reproducing populations in much of the Graham, Page 6 Mississippi River Basin. The introduced range of both carp in the United States continues to grow” (Kolar, Cindy S). In 1970, scientists did not foresee that the Asian Carp had the potential to harm the ecosystems of North America and the fish were not as cautiously confined as they should have been. Types of Asian Carp Bighead Carp: Bighead Carp are “[l]arge filter feeding fish that can weigh up to 110 pounds... [and] have low-set eyes below the mouth and large upturned mouths without barbels. Imported from China in the 1970s for use in aquaculture ponds to control plankton… They eat huge amounts of plankton and detritus. Because they feed on plankton, these fish compete for food with native organisms including mussels, larval fishes, and some adult fish such as paddlefish. This competition for food could result in fewer and smaller sport fish” (Bighead and Silver). Photography by Ronnie Garrison Silver Carp: Graham, Page 7 “Silver carp are olive green in color on their dorsal side and silver on the ventral side. They have a deep, laterally compressed body and a large head. Their eyes are located near the ventral side, which makes them easily distinguishable from other carp. Both dorsal and anal fins are present, but an adipose fin is lacking” (Hypophthalmichthys molitrix Carp). Silver Carp are filter feeders, like the Bighead Carp, and compete for food with other filter feeders. “In addition, boaters have been injured by silver carp because they commonly jump out of the water and into or over boats in response to outboard motors” (Why are they). Silver Carp over Barge Crop: Photography by Michigan Sea Grant Grass Carp: “Grass carp, can eat up to 40% of its body weight in plants every day...Grass carp consume plants, and can drastically change river and shoreline vegetation” (Asian Carp Overview). Graham, Page 8 Grass Carp have been known to cause, “direct and indirect impacts via: competition for food; significant changes in the composition of macrophyte, phytoplankton, and invertebrate communities; interference with the reproduction of other fishes; decreases in refugia for other fishes; modification of preferred habitat; predation or competition when plant food is scarce. Has significantly altered the food web and trophic structure of aquatic systems by inducing changes in plant, invertebrate, and fish communities” (Invasive Species, Asian Carp). Grass Carp: Photography by Jiri Bohdal Black Carp: Black Carp are not filter feeders like Silver and Bighead Carp. They are carnivores and feed on mollusks. Graham, Page 9 “The black carp closely resembles the grass carp Ctenopharyngodon idella. The two species are similar in overall body shape, size and placement of fins. Both black carp and grass carp have very large scales. In contrast to grass carp, the black carp is slightly darker in coloration (not black) and its pharyngeal teeth (throat teeth) are large and similar in appearance to human molars, an adaptation for crushing the shells of mollusks” (Nico, L. G). Mylopharyngodon piceus: Photography by Richardson The term ‘Asian Carp’ is a very loose name that refers to eight different species of Carp, but the four Carp invasive to the freshwater ecosystems of North America are the Black, Silver, Bighead, and Grass Carp. These four invasive Carp are harmful to the food chains of the North American freshwater habitats (see figure C). Graham, Page 10 Figure C-Each of the different Carps eat a different food source and shift the food chain of the ecosystem.; from Bardach, et al. 1972. Aquaculture: The Farming and Husbandry of Freshwater and Marine Organisms. J. Wiley & Sons. Natural Predators to the Asian Carp The only two known predators of the adult Asian Carp live in the Yangzi River in China. These include the Giant Chinese Paddlefish and a species of freshwater dolphin that were known to prey on the adult Asian Carp. “The Chinese river dolphin is a freshwater dolphin and one of the most endangered animals on Earth. It is also known as the Yangtze river dolphin, baiji, white-flag dolphin, and white-fin dolphin” (Chinese River Dolphin). Some scientists consider this dolphin to be extinct because “[a] recent extensive survey of this species resulted in no sightings” (Chinese River Dolphin). Graham, Page 11 Time Photography by AFP/ Getty The other known predator to the adult Asian Carp are Giant Chinese Paddlefish. “Chinese paddlefish are thought by many to be the world's largest freshwater fish, with reports of individuals reaching a mind-boggling 23 feet (7 meters) in length and weighing half a ton” (450 kilograms)....No young Chinese paddlefish have been seen in the wild since 1995, and there have been no sightings of a wild Chinese paddlefish of any size since 2003—leading many to fear that the megafish is already extinct” (Chinese Paddlefish). Both of the natural predators of the adult Asian Carp are critically endangered or possibly even extinct. Graham, Page 12 BBC Photography by Liu Chen Han Predators of the Asian Carp in the States The United States have Paddlefish and dolphins in the freshwater river systems, but they do not prey on adult Asian Carp. “There are no North American fishes large enough to eat an adult Asian carp. White pelicans and eagles, however, have been seen feeding on juvenile or smaller adult Asian carp. Largemouth bass have often been observed feeding on small juvenile Asian carp, and many other native predators probably also feed on them before they grow too large. However, Asian carp produce many offspring which grow quickly and, if conditions are good, rapidly become too large to be eaten by North American predators. Juvenile Asian carp are also known to move into very shallow water where they are inaccessible to many large predators” (Asian Carp Frequently). Juvenile and small Asian Carp have many predators that will eat them, but the larger fish have no known predators in the North American freshwater ecosystems. Graham, Page 13 Special Asian Carp Bodily Functions Asian Carp have a unique body structure that differs from others, mostly because they do not have any teeth. Silver Carp and Bighead Carp have pharyngeal teeth. “The black carp looks very similar to the grass carp. The way to distinguish between the two is the pharyngeal teeth. On black carp the teeth appear molar-like, whereas the grass carp’s teeth have deep parallel grooves in them” (Aquatic Invasive Species). In layman’s terms, pharyngeal teeth are teeth that are like a bony plate instead of the standard human tooth. Pharyngeal teeth “are attached to the gill arches – the same bony supports that serve as attachment points for gill filaments and gill rakers” (Asian Carp, US Fish and Wildlife). Image thanks to Google Images Graham, Page 14 “Asian carp are eating machines! Each fish can consume an astounding 5‐ 40% of its body weight in food each day...Bighead and silver carp lack a true stomach, which requires them to feed almost continuously” (Asian Carp). Adult Silver and Bighead Carp can weigh more than 100 pounds. Special Adaptations of the Asian Carp People often refer to the Asian Carp as the ‘flying fish’ because it jumps out of the water. In actuality, the ‘flying fish’ only refers to the Silver Carp. “Silver carp can jump up to 10 feet out of the water when disturbed by sounds of watercraft. They often jump into boats and can injure boaters, personal watercraft operators, and water skiers” (Bighead and silver carp). The ability to leap out of the water is an aptitude the Asian Carp learned in the United States, however in Asia, the Asian Carp do not jump out of the waters. School of silver carp jumping: Photography by Jason Jenkins Spawning Habits of the Asian Carp Graham, Page 15 “Asian Carp require large, low gradient, turbid rivers to complete their life cycle” (Aitkin, J. Kevin, General). They are known to swim in groups and migrate together. “Spawning happens as soon as the water starts to warm and takes place in turbid waters. The post spawning adults do not die after spawning but they return upriver….Spawning begins when water temperature is greater than 18°” Fahrenheit (Aitkin, J. Kevin, General). Eggs tend to hatch in the main river and the larvae slowly move to smaller creeks, streams, and tributaries. “Bighead carp become sexually mature at 5 to 7 years, black carp at 6 to 11 years, grass carp at 4 to 7 years, and silver carp at 4 to 6 years of age in temperate regions (Nico et al. 2005; Schofield et al. 2005). Eggs are semi buoyant and presumably require flow ‘Temporal and Spatial’ The eggs must be kept above the water and must not sink into the water or else they die” (Open Lake Waters). Asian Carp need a minimum 18°F water temperature to survive until they pass into the juvenile stage. “To survive, Asian carp eggs and larvae require sufficient water temperature for development, water velocity to keep eggs suspended, river length over which to develop while suspended and seek rearing habitat, and appropriate water chemistry” (Aitkin, J. Kevin, General). “Female Asian carp may produce over one million eggs in one season and prefer to spawn in the flowing waters of large rivers” (Asian Carp, Sea Grant). Asian Carp do not parent and nurture their offspring and once they are done breeding they abandon their young. Graham, Page 16 Most of the fish were females and they were mostly eggs - the greenish mass: Photography by Marilyn Kircus Triploid Carp Triploid carp are Grass Carp and Black Carp that have been genetically engineered to harbor three chromosomes instead of two. “Some states permit the introduction of genetically altered, or triploid, carps under the assumption that these animals are sterile, unable to reproduce. However, there Graham, Page 17 are studies that indicated methods of both induction and detection of triploidy may not be 100% effective. The release of diploid organisms into the environment would result. Additionally, some triploid organisms can produce viable gametes and offspring which may allow triploid fishes to establish reproductively sustainable populations” (Invasive and Exotic). These Triploid Carp successfully thrive in the freshwater river ecosystems of North America. They have the capability to crossbreed with other Asian Carp. Although crossbreeding is relatively rare, it still is possible. Locating the Asian Carp Invasion eDNA is a new method of locating and monitoring a species of animals. eDNA can test for an indication of the presence of a certain organism in a specified area. “Dissolved DNA and/or fragments of tissue containing DNA, dubbed environmental DNA (eDNA), remain in suspension [sig] for extended periods” (Mahon, A. Jerde). By using eDNA tests, we can decipher if the Asian Carp are present in other waterways. “eDNA testing is a new but a scientifically accepted method of screening water samples for genetic material originating from an aquatic animal's mucus or excrement”(eDNA tests indicate). “The presence of species can be detected by filtering water samples, and then extracting and amplifying short fragments of the shed DNA” (Lodge, David M, Risk). “Sources of error can come from the molecular method itself and also from the overall monitoring process — from sample design, to sample processing, to interpretation of results” Graham, Page 18 (EPA Notre Dame). eDNA is still a very accurate guess to the presence of a species in a given area. What does eDNA show? “In 2009 a team of scientists from the University of Notre Dame and The Nature Conservancy (TNC) discovered that two species of highly invasive Asian Carp were much closer to the Great Lakes than federal and state officials had realized. Those two species, bighead carp and silver carp, have already done extensive environmental damage to the Illinois River—and much of the Mississippi River—by completely altering the food web in sections of those two major watersheds” (Asian Carp and eDNA). “Water samples from the Mississippi River downstream from the Ford Dam in Minneapolis have tested positive for genetic material from silver carp, indicating the invasive Asian species may be present in the Twin Cities stretch of the river, according to the Minnesota Department of Natural Resources (DNR)” (eDNA Tests indicate). “The Mississippi River eDNA testing was conducted in September by the National Park Service and the DNR after similar testing in June indicated the presence of silver carp in the St. Croix River” (eDNA Tests Indicate). Methods of controlling the Asian Carp Population Graham, Page 19 Kill them with Poison “Rotenone is a chemical that is considered dangerous and chemically toxic. Rotenone is a slow-acting poison which interferes with the electron-transport system in the mitochondria. It acts as both a contact and stomach poison” (Material Fact Sheets). “Rotenone is a natural substance derived from the roots of several tropical and subtropical plants in the bean family. Use of this toxicant in North America began in the 1930s in ponds and lakes as a tool to sample fish populations or to completely eradicate undesirable fish populations. Rotenone is approved for fishery uses by the U.S. Environmental Protection Agency (USEPA)” (Asian Carp FAQ’s). Using Rotenone is an effective way of removing Asian Carp from an area. “Rotenone affects all gill-breathing organisms and if used in the right doses does not have any know bad effects on humans” (Battling Asian Carp). However, Rotenone is considered an emergency tool to remove Asian Carp. Rotenone will kill all fish and any other gilled organisms in the area when used. “The bad side effects of Rotenone include the deaths of channel catfish, and rainbow trout which are both very receptacle to Rotenone" (Material Fact Sheets). “Other uses for Rotenone include insect control, for lice and tick control on pets and in general are used as a pesticide” (Material Fact Sheets). Fish that have been poisoned by Rotenone are extremely dangerous to to consume. “USEPA recommends collecting and burying fish killed by rotenone. The label specifically prohibits the consumption of treated fish” (Asian Carp FAQ’s). Graham, Page 20 Rotenone chemical formula: Alan Wood Electric Barrier Fences “Electric barriers are designed and operated to change an animal’s behavior. Electric barriers are used for control of invasive species, for clearing fish from turbine raceways and intakes, and for collecting runs to allow separating of fish species” (What does). Electric barriers can channel schools of Asian Carp away from certain bodies of water, permanently confining them to a specific area. “An Electric barrier can be calibrated for the size of the fish. Electric barriers work to channel fish in a direction. They do not directly shock the fish but they but they but an electric field in the water that the fish do not want to swim in” (What Does). “Fish have very sensitive touch to the water around them. They feel small changes in the water with a body structure called a lateral line. Fish use it to detect depth and water Graham, Page 21 pressure, prey, predators, current movement, orientation in the current, as well as to avoid collisions” (Lateral Line). “The lateral line system is a collection of small neuromasts located on the skin or just under the skin in fluid-filled canals on the head and body of all fishes. Parts of the lateral organ are modified into electroreceptors, which are organs used to detect electrical impulses. The hair cell allows the fish to feel vibrations in the water and feel slight changes in the water” (Lateral Line). Lateral line system of a Fish: Encyclopædia Britannica, Inc “Electric Barriers are very successful in keeping many fish out of areas because of the sensitive lateral lines that fish have that detect electrical impulses. Graham, Page 22 Other means of controlling Asian Carp include: · Commercial harvesting below places where electric barrier is · Waterway separation measures separate the great lakes and the Mississippi River basin · Research monitor movement and movement patterns of carp · Environmental Deoxyribonucleic Acid: refining eDNA to monitor Asian carp · Enforcement Measures: try to limit the transport of Asian carp · Funding for this protection · And awareness of the carp” (Asian Carp Regional Coordinating Committee) Where are they now? Silver Carp This map shows the most current update on the location of the Silver Carp, the bulk of the Silver Carp are found in the Illinois River and Mississippi River. Silver Carp filter feed on plankton and can hybridize with the Big Headed Carp. They most famous for jumping out of the water and injuring unsuspecting boaters. Graham, Page 23 Guam Saipan Maps from U.S. Geological Survey Big Headed Carp This map shows the most updated location of the Big Headed Carp. The bulk of the Big Headed Carp are found in the Illinois River, Mississippi river, and the Missouri River. These Carp eat plankton and can hybridize with the Silver Carp. Graham, Page 24 Map from U.S Geological Survey Grass Carp This map shows the most current places where Grass Carp are found. Grass Carp invaded the most territory out of all the invasive Asian Carp. They have made it to Lake Michigan, Erie, Huron, and Ontario. Many solutions have been proposed to permanently expel the Grass Carp from the Great Lakes. Graham, Page 25 Alaska Hawaii Guam Saipan Maps from U.S. Geological Survey Black Carp Black Carp have invaded the least amount of territory out of all the invasive Asian Carp and are mostly found in the Illinois River and the Mississippi River. Graham, Page 26 Map from U.S Geological Study How endangered are the Great Lakes? Are the Asian Carp as much of a threat as the media and the public make them to be? The big scare is that these Asian Carp will eventually take over the Great Lakes and uproot entire ecosystems in the Lakes. In reality the Asian Carp are not very suited for the Great Lakes. “Lake Michigan, quagga mussels have eaten 80% of the plankton the Asian carp would depend on for food” (Lam, Tina). Black Head and Silver Carp filter feed and need a steady source of plankton. Lake Michigan would not be able to support a great population of Bighead and Silver Carp. Graham, Page 27 “In Erie the carp might be able to grow for six months of the year” (Lam, Tina). Lake Erie is the warmest of the Great Lakes and is the most livable for Asian Carp. While Lake Erie may be the most ideal locations for a carp to live, this specific lake does not harbor the environmental qualities suitable for laying eggs. For example: Lake Erie has very stagnant waters. Asian Carp would not be able to breed in Lake Erie as well as the other Great Lakes because breeding takes place in mostly turbid waters. Maturing is also a difficult task in Lake Erie because juvenile carp would not be able to easily access the small tributaries and freshwater streams due to the stagnant nature of the waters. “In Lake Superior Asian Carp may only be able to survive two months as the water drops below 59 degrees the fish will lose weight in colder months of the year because it's too cold for them to feed, and their sexual organs will atrophy” (Lam, Tina). Lake Superior is not livable for many fish for it is too cold for them to go about their habitual nature. “Lake Superior is, by surface area, the world's largest freshwater lake and is described as "the most oligotrophic lake in the world" (Linder, Douglas O). Lake Superior also glaciates every year and this deep freeze would not provide a healthy lifestyle for the Asian Carp. Thus, Asian Carp would not be able to breed in Lake Superior very well. Lake Huron and Lake Ontario are also too cold to support the optimum conditions of the Asian Carp. The Great Lakes are not suitable for Asian Carp to thrive in. Lake Superior is an oligotrophic lake; that means there is very little algae content and a dithering amounts of nutrition. This would be a poor lake for filter feeders like the Big Headed Carp and the Silver Carp to live in. Grass Carp would not thrive with such low nutrients and vegetation and Black Carp may not have enough to eat in the Great Lakes. Graham, Page 28 Although Grass Carp reside in the Great Lakes, they have yet to take over the ecosystem. Grass Carp control aquatic vegetation and they eat the herbaceous plants in the area. It is not likely that the Grass Carp would eat out the entirety of the Great Lakes vegetation, but the Grass Carp pose the greatest threat to the lakes currently. However, Grass Carps are restricted by their own breeding needs. They require flowing warm shallow river waters in order to breed and may not optimally reproduce in the Great Lakes. “It seems very unlikely the Asian Carp will survive in the Great Lakes. Asian Carp thrive in the river ecosystems because of the shallow depths in the rivers. The length and shape of the river must be the right length and shape for spawning to occur” (Aitkin, J Kevin, General). There must also be flowing water for the eggs to survive. The eggs must float on top of the water. If they eggs sink, they die. Asian Carp could theoretically survive in the Great Lakes as fully matured fish, then migrate back to the rivers to reproduce. Conclusion “As the Government has poured in $156 million since 2010” (US to Spend) to control the Asian Carp, it seems that the Asian Carp may not be as significant of an issue if the right actions are taken. Individual states are taking action to stop the Asian Carp, however not every state agrees on a uniform budget to spend on the Asian Carp. Some push for more funding and others some push for less. Not only is funding an issue, but not every state agrees with poisoning the Asian Carp with rotenone. Rotenone kills the native fish and Asian Carp making the Asian Carp inedible. Graham, Page 29 “Asian Carp are a good source of protein, they are low in mercury content, and they are high in Omega fatty 3 acids” (Tareen, Sophia, Asian Carp). By utilizing the Carp, we as Americans, now have an abundant food resource that is healthy and plentiful to a standard diet. In addition, these fish have a high Omega fatty 3 acid content, much higher than that of a normal fish. These Omega 3 acids are essential to human health. “The human body does not naturally make Omega 3” (Omega-3) and Omega 3 is necessary for the body to function correctly. Omega-3 fatty acids help reduce inflammation. “Typical American diet tends to contain 14 - 25 times more omega-6 fatty acids than omega-3 fatty acids, which many nutritionally oriented physicians consider to be way too high on the omega-6 side” (Omega3). “Omega 3 is believed to help patients with the following disorders; high cholesterol, high blood pressure, heart disease, diabetes, Rheumatoid arthritis, Systemic lupus erythematosus (SLE), Osteoporosis, depression, bipolar disorder, Schizophrenia, Attention deficit/hyperactivity disorder (ADHD), Cognitive decline (including Alzheimer's disease), skin disorders, Inflammatory bowel disease (IBD), Asthma, Macular Degeneration, Menstrual pain, Colon cancer, Breast Cancer, and Prostate cancer” (Omega-3). Asian Carp should be farmed and researched for their medicinal properties, as well as being incorporated into the diet of modern Americans. Scientists should examine the ways to utilize the Omega 3 and chefs should cook these fish and incorporate them in their dishes. We can use these fish as hunger relief for organizations that assist third world countries, and feed the hungry in America. The Asian Carp can be used as fertilizer, a food for human Graham, Page 30 consumption, and an Omega- 3 supplement. Rotenone should not be used to kill of these creatures, for the Asian Carp are a precious untouched resource. Although invasive, they should be highly praised for the supplements they can provide. New jobs in the fishing industry can be opened and the private farming of these magnificent fish should continue. The overpopulation of these Asian Carp can be solved by harnessing the potential power of Bighead, Black, Silver, and Grass Carp. Graham, Page 31 Works Cited AFP/ Getty. “Farewell to the Yangtze River Dolphin.” Time Science and Space. Photography. 10 Aug. 2007. Web. 3 Jan. 2014. Aitkin, J. 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Acknowledgements: My mentor Mrs. Ervin helped me throughout my draft of this research paper and guided me in the right direction and Nicholas Falduto helped me grammatically polish my final draft of this research paper.