PREPARATION
Ordering Information for Common Supplies
OBJECTIVE
Acquire many of the supplies necessary to begin phagehunting.
HELPFUL TIPS
You may find other vendors have similar products that are perfectly acceptable. These are just
the typical ones we order at Pitt.
Prices are not printed here because they are subject to change so quickly. Most vendors have
pricing available on their websites and reductions for educational institutions.
VENDOR AND PRODUCT LISTS
VWR (www.vwr.com, 800.932.5000)
Product
Catalog #
VWR Superspin Microcentrifuge Tubes, Polypropylene
Middlebrook 7H9 Broth Base
Middlebrook 7H10 Agar
Petri dishes, small, 100mm x 15 mm, standard stackable
Petri dishes, large, 150mm x 15 mm, standard stackable
Syringes with BD Luer-Lock Tip, Sterile, 1 ml
Acrodisc Sterile Syringe Filters with Supor Membrane
(Pall Life Sciences)
15ml centrifuge tube with plug caps, polypropylene, sterile
50ml centrifuge tube with plug caps, polypropylene, sterile
Agar, Bacto
1000ml VWR Erlenmeyer flasks w/ reinforced beaded top
2000ml VWR Erlenmeyer flasks w/ reinforced beaded top
Octagonal Magnetic Stirbars, 5/16” by 1½”
Glycerol, anhydrous (JT Baker)
Costar Disposable Storage Bottle, 250ml capacity
BD Falcon Polypropylene Centrifuge Tubes, 225ml
VWR Vacuum Filtration Systems, 500ml, .22µm pore size,
PES Membrane, Sterile
Spectra/Por 7 Dialysis Membranes , MWCO 1000-50000,
15.9mm diameter
Hydrochloric Acid (EMD)
Hygromycin B
Corning Hoplate/Stirplate, 120V
Page 1 of 3
Size
20170-038
BB11417
DF0627-17
25384-208
25384-139
BD-309628
28143-310
500/pack
100g
500g
1 case (500 plates)
1 case (100 plates)
100/pack
50/pack
93000-022
93000-032
DF0140-15
89000-368
89000-370
58948-150
JT2136-1
28199-758
21008-943
87006-062
500/case
500/case
100g
6/pack
4/pack
-500ml
24/case
48/case
12/pack
25223-901
--
EM-HX0603P-5
80501-088
89090-994
500ml
1g
--
ThermoFisher Scientific (fishersci.com, 800.766.7000)
Product
Catalog #
Boric Acid
Calcium Chloride (Anhydrous)
Cesium Chloride
Corning Milk Dilution Bottles, ungraduated, narrow mouth
Dextrose
EDTA Disodium Salt
Ethanol, 200 proof
Magnesium Sulfate (Anhydrous)
Millipore Steriflip Disposable Vacuum Filter Units
(.22µm pore size)
Seakem LE Agarose (Cambrex)
Sodium Acetate Trihydrate
Sodium Chloride
Tween 80
Size
BP168-500
C614-500
BP210-100
02-943-5
D16-500
BP120-500
-M65-500
SCGP00525
500g
500g
100g
12/pack
500g
500g
-500g
25/pack
BMA50004
S209-3
BP358-1
T164-500
500g
3kg
1kg
500ml
Sigma (www.sigma-aldrich.com, 800.325.3010)
Product
Catalog #
Carbenicillin Disodium Salt (CB)
Cyclohexamide (CHX)
Ethidium Bromide 10mg/ml
Leucine
Polyethylene Glycol (PEG)
TRIZMA base
C1389
C7698
E1510
L8000
P2139
T1503
Size
5g
1g
10ml
25g
500g
500g
BellCo (www.bellcoglass.com, 800.257.7043)
Product
Catalog #
50 ml shake flask with baffles
125 ml shake flask with baffles
250 ml shake flask with baffles
500 ml shake flask with baffles
18 mm stainless steel closures
25 mm stainless steel closures
38 mm stainless steel closures
2540-00050
2540-00125
2540-00250
2540-00500
2005-00018
2005-02512
2005-03812
Size
--------
New England Biolabs (www.neb.com, 800.632.5227)
Product
Catalog #
BamH1 restriction enzyme, 20,000 units/ml
Cla1 restriction enzyme, 5,000 units/ml
EcoR1 restriction enzyme, 20,000 units/ml
HaeIII restriction enzyme, 10,000 units/ml
HindIII restriction enzyme, 10,000 units/ml
R0136S
R0197S
R0101S
R0108S
R0104S
Page 2 of 3
Size
10,000 units
1,000 units
10,000 units
3,000 units
10,000 units
Beckman Coulter (www.beckmancoulter.com, 800.742.2345)
Product
Catalog #
Quick-Seal Polyallomer Ultracentrifuge Tubes, 13.5 ml,
16 x 76 mm
342413
Size
50/box
Spectrum (www.spectrumchemical.com, 800.772.8786)
Product
Catalog #
Albumin, Bovine Serum (BSA), Fraction V
A3611-100GM
Size
100g
Aurical Company (www.auricalcompany.com, 800.615.8870)
Product
Catalog #
Pourite Anti-Bubble Additive
8-505
Size
60 ml
USA Scientific (www.usascientific.com, 800.522.8477)
Product
Catalog #
Screw-Cap Microcentrifuge Tubes, 1.5 ml
1415-8799
Page 3 of 3
Size
500/pack