World’s Poultry Science Association –
Bangladesh Branch
9th International Poultry Show and Seminar
19 February to 21 February, 2015
Instructions for authors
1. Compose your manuscript in English in MS Word, using “Times New Roman” font, on A4
paper layout with 2.5 cm (1 inch) margins on the left and right and 3.8 cm (1.5 inch) margin
on the top and bottom.
2. Type the manuscript single-spaced. Use an extra line space between the sections. Forced line
break and page break should not be used.
3. 12-point Times New Roman font should be used all through except the title and references,
which should be 18-point and 10-point, respectively. The title, authors’ name, address,
abstract and all section headings should be in bold face. Please see the attached “Sample
Manuscript” for an example.
4. Manuscripts including tables, illustrations and references must not exceed 6 printed pages (for
papers accepted for oral presentations) or 3 printed pages (for papers accepted for poster
presentation).
5. Insert Tables into the text at appropriate location, preferably in portrait format, not landscape.
Oversized table must be avoided. Raw data should be summarized into a concise table.
6. Do not insert Figures in the text. Instead, submit figures on separate page(s) at the end of the
manuscript or submit as separate files. The editor will insert the figures in the text at
appropriate location. Colored figures may be submitted, which will appear in color in the
electronic version (in the website) but in gray scale in the printed version.
7. All tables and figures must be numbered. Each table must have a caption above the table,
while the figures must have caption below the figure.
8. The research papers should have the following items or sections:
Title
Authors’ panel
–
Underline the name of presenting author; identify the corresponding author with an
asterisk
Address panel
–
Give the address(es) of the place(s) where the study was conducted (which could be
different from the present address)
–
If the study was conducted at more than one place, then link the name of authors with
the respective place of work using superscript numbers.
–
If the present address of the author is different from the place of work, then the
present address may be given as a footnote using a symbol, such as §
–
Give also the E-mail address of the corresponding author
Abstract (Maximum 250 words)
Keywords (Maximum eight)
Introduction
Materials and methods
Results and discussion (as a single section or two separate sections)
Conclusion (if any)
References
–
All references cited in the text must be listed alphabetically in the References section. For
citation and listing of the references, follow the style shown in the attached Sample
9. Review or advocacy articles should also have title, authors’ panel, address panel, abstract,
keywords and references as that in a research paper, but the main text in the body may be
arranged under different headings, as appropriate
10. The paper must not purposefully promote any specific product, breed, etc. or must not
generate any commercial conflict of interest. If the material under study is a commercial
product, use generic name or a code instead of trade name.
A sample for example (the text is incomplete)
Molecular epidemiology of highly pathogenic avian
influenza viruses (H5N1) in Bangladesh from 2007 to 2012
M.E. HAQUE1*, M. GIASUDDIN2, M. NOORUZZAMAN1, E.H. CHOWDHURY1 and
M.R. ISLAM1
1Department
of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural
University, Mymensingh, Bangladesh; 2National Reference Laboratory for Avian
Influenza, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh
*Corresponding Author: ehaque_vs@hotmail.com
Abstract
--------Key words: highly pathogenic avian influenza (HPAI), molecular epidemiology, RT-PCR,
sequencing, phylogenetic tree
Introduction
--human lives (http://www.who.int/influenza/human_animal_interface/EN_GIP_20120810).
Adaptation of HPAI in human host through mutation or recombination could give rise to
“bird flu” pandemic in human as well (Alexander, 2007) ---------Materials and Methods
---
Results and Discussion
All the isolates yielded positive amplification of 545 bp fragment of H5 gene and 343 bp
fragment of N1 gene on RT-PCR, as expected (Fig1).
Fig1: Amplification of 545 bp fragment of H5 gene
All the Bangladeshi isolates that were obtained from chickens during the period from 2007 to
2010 clustered with the strain representing clade 2.2, as described earlier (Haque et al.,
2011). On the other hand, among the ten Bangladeshi isolates of 2011 included in the present
analysis, only three fell into clade 2.2, one belonged to clade 2.3.4 and the remaining six were
related to the strain representing clade 2.3.2, indicating the co-circulation of viruses of three
clades as suggested by Islam et al. (2012). All the six isolates of 2012 also clustered in clade
2.3.2. Interestingly, these Bangladeshi isolates of clade 2.3.2, along with the recent isolates
from the People’s Republic of China, Viet Nam, Lao, the Republic of Korea, Japan,
Mongolia, Russia and Nepal, (Smith et al., 2004, Chen et al.; 2004; Jiang et al., 2007, Kim et
al., 2008 and Kang et al., 2011) formed a distinct new cluster within clade 2.3.2, which has
recently been designated as clade 2.3.2.1.
Conclusion:
H5N1 HPAI viruses of clades 2.3.2.1 and 2.3.4 have been newly introduced in poultry of
Bangladesh in 2011 in addition to the clade 2.2 already circulating since 2007. ---References
ALEXANDER, D. J. (2007) An overview of epidemiology of avian influenza. Vaccine 5:5637-5644.
EASTERDAY, B.C., VIRGINIA, S.H. and HALVORSON, D.A. (1997) Influenza. In: Diseases of
Poultry,10th edn., Edited by Calnek et al., Iowa State University Press, Ames, Iowa. pp. 583-605.
HAQUE, M.E., PARVIN, R., CHOWDHURY, E.H., BHUIYAN, A.R., NOOR, M., GIASUDDIN, M.,
SAMAD, M.A. and ISLAM, M.R. (2011) Phylogenetic analysis of HA gene of H5N1 avian influenza
virus isolates from Bangladesh reveals only a single introduction. Proceedings of the 7th International
Poultry Show and Seminar of WPSA-BB, Dhaka, Bangladesh 25-27 March, 2011, pp 69-74.
HOFFMANN, E., STECH, J., GUAN, Y., WEBSTER, R.G. and PEREZ, D.R. (2001). Universal primer set
for the full-length amplification of all influenza A viruses. Archives of Virology 146: 2275-2289.
ISLAM, M.R., HAQUE, M.E., GIASUDDIN, M., CHOWDHURY, E.H., SAMAD, M.A., PARVIN, R.,
NOORUZZAMAN, M., RAHMAN, M.M and MONOURA, P. (2012) New introduction of Clade
2.3.2.1 avian influenza virus (H5N1) into Bangladesh. Transboundary and Emerging Diseases 59: 460–
463
KANG, H.M., BATCHULUUN, D., KIM, M.C., CHOI, J.G., ERDENE-OCHIR, T.O., PAEK, M.R.,
SUGIR, T., SODNOMDARJAA, R., KWON, J.H. and LEE, Y.J. (2011) Genetic analyses of H5N1
avian influenza virus in Mongolia, 2009 and its relationship with those of eastern Asia. Veterinary
Microbiology 147:170–175.
WHO (2007) Recommendations and laboratory procedures for detection of avian influenza A (H5N1) virus in
specimens from suspected human cases. World health Organization, August 2007.Available at
http://www.who.int/csr/disease/avian_influenza/guidelines/RecAIlabtestsAug07.pdf
(accessed: September 17, 2011).
NRC (NATIONAL RESEARCH COUNCIL) (1994) Nutrient Requirements of Chickens. In: NRC (Eds)
Nutrient Requirements of Poultry, 9th Revised Ed. pp.19- 34, National Academy Press, Washington,
D.C.
STADELMAN, W. J. (1977). Quality preservation of shell egg. In Egg Science and Technology (2 nd edition).
SAMINATHAN, M. (2011) Effects of dietary probiotic, prebiotic and symbiotic supplementations on growth
performance of broilers. Master of Science Thesis, Universiti Putra Malaysia, Serdang, Malaysia.