World’s Poultry Science Association – Bangladesh Branch 9th International Poultry Show and Seminar 19 February to 21 February, 2015 Instructions for authors 1. Compose your manuscript in English in MS Word, using “Times New Roman” font, on A4 paper layout with 2.5 cm (1 inch) margins on the left and right and 3.8 cm (1.5 inch) margin on the top and bottom. 2. Type the manuscript single-spaced. Use an extra line space between the sections. Forced line break and page break should not be used. 3. 12-point Times New Roman font should be used all through except the title and references, which should be 18-point and 10-point, respectively. The title, authors’ name, address, abstract and all section headings should be in bold face. Please see the attached “Sample Manuscript” for an example. 4. Manuscripts including tables, illustrations and references must not exceed 6 printed pages (for papers accepted for oral presentations) or 3 printed pages (for papers accepted for poster presentation). 5. Insert Tables into the text at appropriate location, preferably in portrait format, not landscape. Oversized table must be avoided. Raw data should be summarized into a concise table. 6. Do not insert Figures in the text. Instead, submit figures on separate page(s) at the end of the manuscript or submit as separate files. The editor will insert the figures in the text at appropriate location. Colored figures may be submitted, which will appear in color in the electronic version (in the website) but in gray scale in the printed version. 7. All tables and figures must be numbered. Each table must have a caption above the table, while the figures must have caption below the figure. 8. The research papers should have the following items or sections: Title Authors’ panel – Underline the name of presenting author; identify the corresponding author with an asterisk Address panel – Give the address(es) of the place(s) where the study was conducted (which could be different from the present address) – If the study was conducted at more than one place, then link the name of authors with the respective place of work using superscript numbers. – If the present address of the author is different from the place of work, then the present address may be given as a footnote using a symbol, such as § – Give also the E-mail address of the corresponding author Abstract (Maximum 250 words) Keywords (Maximum eight) Introduction Materials and methods Results and discussion (as a single section or two separate sections) Conclusion (if any) References – All references cited in the text must be listed alphabetically in the References section. For citation and listing of the references, follow the style shown in the attached Sample 9. Review or advocacy articles should also have title, authors’ panel, address panel, abstract, keywords and references as that in a research paper, but the main text in the body may be arranged under different headings, as appropriate 10. The paper must not purposefully promote any specific product, breed, etc. or must not generate any commercial conflict of interest. If the material under study is a commercial product, use generic name or a code instead of trade name. A sample for example (the text is incomplete) Molecular epidemiology of highly pathogenic avian influenza viruses (H5N1) in Bangladesh from 2007 to 2012 M.E. HAQUE1*, M. GIASUDDIN2, M. NOORUZZAMAN1, E.H. CHOWDHURY1 and M.R. ISLAM1 1Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh; 2National Reference Laboratory for Avian Influenza, Bangladesh Livestock Research Institute, Savar, Dhaka, Bangladesh *Corresponding Author: [email protected] Abstract --------Key words: highly pathogenic avian influenza (HPAI), molecular epidemiology, RT-PCR, sequencing, phylogenetic tree Introduction --human lives (http://www.who.int/influenza/human_animal_interface/EN_GIP_20120810). Adaptation of HPAI in human host through mutation or recombination could give rise to “bird flu” pandemic in human as well (Alexander, 2007) ---------Materials and Methods --- Results and Discussion All the isolates yielded positive amplification of 545 bp fragment of H5 gene and 343 bp fragment of N1 gene on RT-PCR, as expected (Fig1). Fig1: Amplification of 545 bp fragment of H5 gene All the Bangladeshi isolates that were obtained from chickens during the period from 2007 to 2010 clustered with the strain representing clade 2.2, as described earlier (Haque et al., 2011). On the other hand, among the ten Bangladeshi isolates of 2011 included in the present analysis, only three fell into clade 2.2, one belonged to clade 2.3.4 and the remaining six were related to the strain representing clade 2.3.2, indicating the co-circulation of viruses of three clades as suggested by Islam et al. (2012). All the six isolates of 2012 also clustered in clade 2.3.2. Interestingly, these Bangladeshi isolates of clade 2.3.2, along with the recent isolates from the People’s Republic of China, Viet Nam, Lao, the Republic of Korea, Japan, Mongolia, Russia and Nepal, (Smith et al., 2004, Chen et al.; 2004; Jiang et al., 2007, Kim et al., 2008 and Kang et al., 2011) formed a distinct new cluster within clade 2.3.2, which has recently been designated as clade 220.127.116.11. Conclusion: H5N1 HPAI viruses of clades 18.104.22.168 and 2.3.4 have been newly introduced in poultry of Bangladesh in 2011 in addition to the clade 2.2 already circulating since 2007. ---References ALEXANDER, D. J. (2007) An overview of epidemiology of avian influenza. Vaccine 5:5637-5644. EASTERDAY, B.C., VIRGINIA, S.H. and HALVORSON, D.A. (1997) Influenza. In: Diseases of Poultry,10th edn., Edited by Calnek et al., Iowa State University Press, Ames, Iowa. pp. 583-605. HAQUE, M.E., PARVIN, R., CHOWDHURY, E.H., BHUIYAN, A.R., NOOR, M., GIASUDDIN, M., SAMAD, M.A. and ISLAM, M.R. (2011) Phylogenetic analysis of HA gene of H5N1 avian influenza virus isolates from Bangladesh reveals only a single introduction. Proceedings of the 7th International Poultry Show and Seminar of WPSA-BB, Dhaka, Bangladesh 25-27 March, 2011, pp 69-74. HOFFMANN, E., STECH, J., GUAN, Y., WEBSTER, R.G. and PEREZ, D.R. (2001). Universal primer set for the full-length amplification of all influenza A viruses. Archives of Virology 146: 2275-2289. ISLAM, M.R., HAQUE, M.E., GIASUDDIN, M., CHOWDHURY, E.H., SAMAD, M.A., PARVIN, R., NOORUZZAMAN, M., RAHMAN, M.M and MONOURA, P. (2012) New introduction of Clade 22.214.171.124 avian influenza virus (H5N1) into Bangladesh. Transboundary and Emerging Diseases 59: 460– 463 KANG, H.M., BATCHULUUN, D., KIM, M.C., CHOI, J.G., ERDENE-OCHIR, T.O., PAEK, M.R., SUGIR, T., SODNOMDARJAA, R., KWON, J.H. and LEE, Y.J. (2011) Genetic analyses of H5N1 avian influenza virus in Mongolia, 2009 and its relationship with those of eastern Asia. Veterinary Microbiology 147:170–175. WHO (2007) Recommendations and laboratory procedures for detection of avian influenza A (H5N1) virus in specimens from suspected human cases. World health Organization, August 2007.Available at http://www.who.int/csr/disease/avian_influenza/guidelines/RecAIlabtestsAug07.pdf (accessed: September 17, 2011). NRC (NATIONAL RESEARCH COUNCIL) (1994) Nutrient Requirements of Chickens. In: NRC (Eds) Nutrient Requirements of Poultry, 9th Revised Ed. pp.19- 34, National Academy Press, Washington, D.C. STADELMAN, W. J. (1977). Quality preservation of shell egg. In Egg Science and Technology (2 nd edition). SAMINATHAN, M. (2011) Effects of dietary probiotic, prebiotic and symbiotic supplementations on growth performance of broilers. Master of Science Thesis, Universiti Putra Malaysia, Serdang, Malaysia.