Agro-technology of genetically improved and economically important medicinal and aromatic plants National Botanical Research Institute, Lucknow-226 001 The increasing global interest and expanding market of herbal drugs have led to their introduction into cultivation to meet the demand at reasonable economic price. Cultivation of medicinal plants can also facilitate in maintaining standards - in quality, potency and chemical composition of the produce. The active principles of the plant are generally secondary metabolites and their biosynthesis, though controlled genetically, is strongly affected by soil, climatic and agricultural factors. It is, therefore advised not to use chemical fertilizers, insecticides and pesticides in cultivation of medicinal plants. Application of chemical fertilizers may alter the desired chemical composition of medicinal plants and application of herbicides and other plant protection chemicals may lead to accumulation of their residue in the plant. It will affect the chemical composition of the plant, which may cause great health hazards to the human being, besides polluting the environment. The organic cultivation shall ensure sustainable development not only in terms of production of medicinal plants, but also in socio-economic and ecological parameters. Status of work at NBRI Conservation The institute has developed medicinal plant gardens for ex situ conservation of medicinal plants resources of northern plains. More than 300 species of medicinal and aromatic plants are being conserved in the garden. These garden are located in the Botanic Garden and Farm Sites. Propagation The germplasm, collected from different regions is conserved in the garden. Simultaneously, phonological observations are recorded for these plants. Superior plants are selected on the basis of growth and economic yield and propagation techniques are developed for superior types. The planting material of these plants are made available to interested researchers, farmers and other organizations. Cultivation The cultivation of medicinal and aromatic plants (MAPs) is being undertaken at the Farm Sites of the institute. The soil of the site generally falls in the category of alkaline soils, found abundantly in this part of the country. The group is working on development of agro-techniques for cultivation of MAPs in different cropping systems with main emphasis on various inter-cropping models with trees, shrubs and herbs adopting the principles of organic farming. The group also provides technical know-how and support by way of supply of good quality, genuine planting material to entrepreneurs as well as researchers. The agro-technology for cultivation of some of the promising species has been standardized by the institute. The prominent among these include Abelmoschus moschatus, Ammi majus, Asparagus racemosus, Chamomilla recutita (Chamomile), Curcuma sp., Cymbopogon sp., Desmodium gangeticum, Hemidesmus indicus, Pueraria tuberosa, Mucuna pruriens, Ocimum sp., Polianthes tuberosa, Rosa damascena, Ureria picta, and Vetiver zizanioides. B. Scope of utilizing wastelands There is great scope of cultivation of medicinal and aromatic plants on marginal soils/ wastelands without disturbing the food priorities for the increasing population of the country. A large number of species belonging to this category have been screened for their adaptability and feasibility for large-scale cultivation on alkaline soils, extensively occurring in the Gangetic alluvial plains of Uttar Pradesh. For utilising these soils, knowledge about the availability of nutrients, toxic concentrations of elements, soil microbial population and physical condition of the soil is essential. The cultivation on these soils requires special agronomic management and amelioration before starting the cultivation. The plants have varying degree of tolerance to such soils and the cropping systems shall be adopted accordingly. Therefore, concerted efforts are required for R&D work on these crops for their cultivation in wastelands to release the ever increasing pressure on normal soils. Keeping in view the above facts, a number of experiments have been conducted at NBRI which have revealed the suitability of some of these crops to sodic soils. The special agronomic management and amelioration is required for these soils before starting the cultivation. Under a sponsored project on screening suitable medicinal and aromatic plants for sodic soils, the field experiments were conducted on 30 medicinal and aromatic crop plants, at three sites, having 8.47, 8.86 and 9.39 pH. Among the tested medicinal crop plants, Vacha (Acorus calamus), Brahmi (Bacopa monnieri), Bhringraj (Wedelia calendulacea), Babchi (Psorelia corylifolia) and Chamomile (Chamomilla recutita) were classified as tolerant to sodic soil conditions. The semi-tolerant crops included Khurasani ajowain (Hyoscyamus niger), Kasni (Cichorium intybus), Atrilal (Ammi majus), Kewanch (Mucuna pruriens), Kasoori methi (Trigonella corniculata), Pippali (Piper longum), Kalmegh (Andrographis paniculata), Saunf (Foeniculum vulgare), Chandrasoor (Lepedium sativum), Shatawar (Asparagus racemosus), Isabgol (Plantago ovata), Shalparni (Desmodium gangeticum) and Gorakhmundi (Spilanthes acmella). Safed moosali (Chlorophytum borivillianum), Ajmoda (Apium graveolens), Pristhaparni (Ureria picta), Ajowain (Trachyspermum ammi), Kulanjan (Alpinia galanga), Haldi group (Curcuma amada, C. longa and C. zeoderia) were found sensitive. C. Bio-prospecting of Withania somnifera BIOPROSPECTING OF WITHANIA SOMNIFERA More than 100 accessions were collected from different geographical locations across India. These were analyzed for chemo-profiling using marker compounds (Withaferin A, Withanone, Withanolide D and Glycowithanolide). Among these five accessions were distinct in their chemo-profiling and selected for pharmacological studies. Data from chemo-profiling with marker compounds Accessions Withaferin A Withanone Withanolide D Glycowithanolide Accession-1 ++++ ++++ - - Accession-2 ++++ - - - Accession-3 - ++++ - - Accession-4 - - ++++ ++++ Experimental hybrid ++++ ++++ ++++ - Pharmacological properties of marker compounds under investigation Withaferin A Withanone Withanolide D Glycowithanolides Anti-tumour, Anti inflammatory, Immunosupressive, antiarthritis Effect on CNS Antitumour, antiinflammatory, immunosupressant Immunostimulant, antistress, phagocytotic, antioxidant, antistress D. Cropping Systems Research on Medicinal Plants A significant constraint in commercial cultivation of medicinal and aromatic plants is availability of cultivable land. In view of the increasing demand for food grains production with increasing population, there is no scope of bringing cultivable lands under cultivation of this category of plants. In order to derive maximum benefit of soil moisture, nutrients and other inputs, medicinal crops can be grown as inter-crops. Intercropping of medicinal crops with crops of same category or with food and vegetable crops, requiring wider rows or with medicinal and fruit trees will give extra income without affecting growth and yield of the main crop. Keeping in view the above facts, a number of experiments have been conducted at NBRI to study the feasibility of their cultivation in different inter-cropping systems. Medicinal and aromatic plants for water-logged sites Acorus calamus (Vacha) Bacopa monnieri (Brahmi) Inter-cropping medicinal and aromatic plants Curcuma longa (Turmeric) grown with Teak Other MAPs as inter-crop with Teak Medicinal and aromatic plants for sodic wastelands Chamomilla recutita (Chamomile): An excellent choice for sodic soils MAPs growing moderately well in sodic wastelands Andrographis paniculata (Kalmegh) Asparagus racemosus (Shatawari) MAPs growing moderately well in sodic wastelands Mucuna pruriens (Kewanch) Withania somnifera (Ashwagandha)