NORTHERN STRIPE RUST MANAGEMENT: AN EVALUATION OF SEED TREATMENT EFFICACY AND BENEFITS Richard Daniel, Northern Grower Alliance (NGA) Key words Wheat, stripe rust, leaf disease, fungicides, yield GRDC code NGA00001: validation and integration of new technology through grower groups in north-west NSW and south-west Queensland grain growing zones Take home messages Plant establishment counts showed good levels of crop safety from the seed treatment under a range of conditions Seed treatment still provided significant suppression of stripe rust in the variety Lang, over 100 days after planting No impact of seed treatment on yield in the absence of stripe rust No yield impact from either seed treatment or foliar use where stripe rust severity was very low and moisture/ heat caused major yield limitations Seed treatment will be a valuable risk management tool in situations of moderate to high stripe rust risk (susceptible varieties/ higher yield potentials) Background In recent years stripe rust has re-emerged as a disease of significance across all Australian grain growing areas. Plant breeding incorporating resistant germplasm represents the most effective method of long term disease management. However fungicides are an additional tool that will enable disease management in varieties without effective genetic resistance. Currently there are no varieties with effective stripe rust resistance combined with useful tolerance to crown rot. Until such varieties are released, northern growers and advisers need to select varieties firstly on the basis of crown rot risk and then manage for stripe rust accordingly. Research conducted in 2005 by Steven Simpfendorfer, NSW DPI Tamworth, demonstrated significant yield benefits from the use of ‘at planting’ treatments for stripe rust management on susceptible varieties (ratings 1-4). Two planting options provided greatest benefit; Jockey® - a long acting seed treatment and Impact® - a fertilizer treatment. Although disease onset did not occur until GS49 (awn peep), yield benefits of 17%-27% were obtained from the use of Jockey (at 300-450 mL/100 kg seed) and 19% from Impact. The level of yield benefit was similar to the 23% obtained from a single high rate fungicide application at GS49. This project focused on examining the crop safety, length of efficacy and economic benefit available from Jockey under northern conditions. The aim was to validate the 2005 NSW DPI results and generate additional data to enable improved advice for regional stripe rust management. Methods Five commercial replicated trials were established in 2006 in the Wyaga, Billa Billa, Boomi, Garah and Edgeroi districts. Trial co-operators were selected who were growing moderately susceptible varieties (ratings 4-5) and intended to treat with Jockey. Jockey was evaluated in all trials at the 300 mL/100 kg seed application rate. In each situation a small amount of the same seed batch was left untreated. Varieties evaluated were Sunco and Lang (both rating 5) and EGA Wylie (rating 4). Individual plot sizes ranged from 12-72 m wide x full field length with row spacings from 22-40 cm. Trials were assessed for plant establishment, stripe rust efficacy and taken through to commercial harvest for grain yield and quality. Weigh bin yields were taken at 4 of the 5 sites. The Wyaga site was not harvested due to extremely low yields (<200 kg/ha) and high site variability from non-treatment effects. Results Plant Establishment All sites were assessed between 17 and 34 days after planting. Relatively deep planting was evident at both the Boomi and Garah sites (seed 7-10 cm below surface) with moderate moisture stress already evident at the Wyaga site. There was no significant difference in establishment counts evident at any location or over the entire series of trials (Figure 1). 160 Untreated Jockey 300 mL 140 Plant stand/ sq m 120 100 80 60 40 20 0 NGA0601 Wyaga NGA0602 Billa Billa NGA0603 Boomi NGA0604 Garah NGA0605 Edgeroi No significant difference in any trial Figure 1: Plant establishment across all sites Disease progression No stripe rust was found at the Wyaga site with trace levels only at Billa Billa, Boomi and Garah. Significant levels of stripe rust only occurred at the Edgeroi site (Figure 2). Disease levels at the end of September combined with crop potential at that stage resulted in a preventative foliar spray being applied to half of each plot on October 2. Hot and dry conditions during early October however largely stopped any further spread of the disease. Stripe rust at all sites was confirmed as the WA pathotype (134 E16 A+). This trial represents a situation with late onset of disease and both very low stripe rust incidence and severity. 5 Flag-3 Flag-2 Flag-1 Flag Severity (% leaf area infected) 4 3 2 1 0 GS33-39 (Flag -1 to flag emerged) GS55 (50% head emerged) GS77 (late milk) 20 Sep (88 days after planting) 30 Sep (98 days after planting) 17 Oct (115 days after planting) No disease evident Disease evident on 14% of leaves Disease evident on 15% of leaves Figure 2: Stripe rust progression in variety Lang (5) NGA0605 Fungicide performance Figure 3 shows the performance of the range of treatments at both assessments. Jockey alone still provided significant reductions in stripe rust severity up to 115 days after planting. The application of Tilt® 250 mL/ha, on Oct 2, on plots where seed had been untreated provided equivalent levels of stripe rust management to the seed treatment alone. No disease was found in plots where Jockey had been used at planting and then followed up with Tilt. However this was not significantly different to the suppression achieved by the seed treatment or foliar application alone. 0.3 Severity (ave % leaf area infected) DAS 98 DAS 115/ 1DAA 15 a 0.2 0.1 b b b 0.0 Untreated Jockey 300 mL UTC + Tilt 250 mL Jockey 300 mL + Tilt 250 mL Treatments sharing the same letter (in each timing) are not significantly different LSD value: DAS 113/1DAA 15 = 0.11% Figure 3: Fungicide efficacy on stripe rust in variety Lang (5) NGA0605 Grain yield and quality There was no significant difference in grain yield between Jockey and the untreated at any of the four sites harvested (Figure 4). There was no apparent treatment impact on grain quality. 3500 Untreated Jockey 300 mL 3000 Grain yield kg/ha 2500 2000 1500 1000 500 0 NGA0602 Billa Billa NGA0603 Boomi NGA0604 Garah No significant difference in any trial NB No stripe rust at sites NGA0602/03 and 04, very low incidence and severity at NGA0605 NGA0605 Edgeroi Figure 4: Grain yield across all sites There was no significant difference in grain yield between any of the fungicide treatments and the untreated at the Edgeroi site (Figure 5). There was no apparent treatment impact on grain quality. 4000 Grain yield kg/ha 3000 2000 1000 0 Untreated Jockey 300 mL Untreated + Tilt 250 mL Jockey 300 mL + Tilt 250 mL No significant difference between treatments Figure 5: Grain yield in variety Lang (5) NGA0605 Commercial benefit – combined data Figure 6 below details the net benefit/cost of the seed treatment across all trial sites. Net benefit has been calculated on two different grain prices after deduction of product cost. Across all trials the Jockey treated area resulted in a net cost of $13-14/ha. 5 @ $270/t (2006 del) @ $200/t 0 Net $/ha benefit -5 -10 -15 ave -$14/ha -20 -25 ave -$13/ha -30 -35 NGA0602 Billa Billa NGA0603 Boomi NGA0604 Garah NGA0605 Edgeroi NB No stripe rust at sites NGA0602/03 and 04, very low incidence and severity at NGA0605 Figure 6: Combined net benefit of seed treatment use Conclusions The plant establishment data showed good crop safety levels from Jockey under a range of northern conditions. This supports results from other regions. Although stripe rust was present in northern NSW in 2006 it was generally at very low levels with non-conducive conditions for disease epidemics. Efficacy data was only generated from the Edgeroi site. Jockey at 300 mL clearly still provided suppression of stripe rust in excess of 100 days after planting on the variety Lang (rating 5). This supports the length of activity seen by NSW DPI at Tamworth in 2005. The absence of yield responses was not unexpected given the seasonal conditions. Jockey is a product that provides extended protection against stripe rust. In situations where stripe rust is only present in trace levels (Billa Billa, Boomi and Garah) no yield benefit would be expected. At the Edgeroi site, stripe rust levels looked to be taking off at the end of September but moisture and heat stress became the major yield limitations not disease. Yield results are likely to have been different if a reasonable end to the season had been experienced. Acknowledgements Sincere thanks to the growers and consultants involved in this trial work. Contact details Richard Daniel Northern Grower Alliance Ph: 07 4698 7983 Email: richard.daniel@nga.org.au