Observation of Snail Behavior Background The purpose of this lab is to use the scientific method in studying snail behavior. We will be observing the snail’s response to various stimuli, and collecting our observations. We will then interpret those results by explaining why these responses would be useful to the snail for surviving in its natural environment. The natural environment of a snail involves humidity. The snails prefer cool, moist, and dark environments during the day and they feed during the night. Before the lab you are to collect a garden snail. Keep the snail in a container without any food sources in a cool place: the refrigerator. When you collect your snail record your observations of its habitat and what time you collected the snail. Procedure Observations: Record your observations of the snail’s behavior to the various stimuli and place your results in a table. Put your snail in a shallow bowl of cool water and keep it there until it becomes active. Snail Behavior 1) Locomotion (a) Place an active snail in a bowl and while holding the bowl above your head, observe how the snail moves. Record your observations. The snail leaves behind a trail of mucus – why is this mucus useful to the snail? (b) Turn the bowl over and place the snail on the bottom of the bowl. Allow the snail to move freely over the surface of the bowl. How does the snail respond to the edge of the bowl? Record your observations. (c) Rate of locomotion: You will measure the rate of locomotion of the snail in millimeters traveled per minute on three different surfaces. Fore each surface make three trials and average the rate of movement for each surface. Record your observations in a table. The three surfaces are: table top, sand paper, cotton. 2) Movement in response to gravity (geotaxis) (a) Attach a string to the upper part of the snail’s shell with some tape. Place the snail on a clean table top. When the snail has begun to move steadily in one direction, pull the string at right angles to the direction in which the snail is moving. Pull firmly enough to displace the shell on the snail’s back, (but not hard enough to injure the snail). Pull to the snail’s left and pull the snail’s right and observe how pulling altered the snail’s movement. Record your observations. (b) Place your snail on the bottom of a bowl and turn the bowl so the snail is moving on a vertical surface. (i) After the snail has established a direction of movement, rotate the bowl 90 degrees to the direction of its movement and record its newly established direction. (ii) Repeat, but rotate the bow 180 degrees and record its newly established direction. (iii) When the snail has once again established its direction of movement to the vertical surface, pull the string in the direction of the movement. Record any changes in the direction of movement. 3) Movement in response to chemicals (chemotaxis) The snail ahs 2 pairs of tentacles which it uses to sample its surroundings. (a) Crush some lettuce and place a thin streak of its juice across a clean, dry table top. Place the snail on the table so its direction of movement is toward the juice. Record your observations of the snail’s response. (b) Dampen a cotton swab with water and bring it close to the tentacles. Record your observations. (This is a control – testing the snail’s response to the damp cotton swab). (c) Dampen a cotton swab with vinegar and test the snail’s response. Record your observations. (d) Repeat, but this time dampen your cotton swab with amyl alcohol and record your observations. 4) Movement in response to light (phototaxis) Place the snail on a dry, clean surface and dim the lights in the room. Shine the light from the dissecting microscope on the snail so it has its choice to travel on a illuminated or a darkened surface. Record your observations. Summarizing your data: Make a summary table of your observations. Locomotion: What is the response of the snail’s locomotion to different surfaces? Movement in response to gravity: Does the snail exhibit a positive (towards) or negative (away from) response to gravity? Movement in response to chemicals: Does the snail like lettuce, water, vinegar, amyl alcohol? Movement in response to light: What is the snail’s response to light? Analyzing your data: You will interpret your data by answering the following questions: How does the snail’s behavior help it survive in its environment? Remember your answer must be supported by your observations. Use your observations from each of the lab – locomotion, movement in response to gravity, chemical and light. You should also include your observations of the snail in its natural environment.