Principles of Learning Laboratory (PSYC 301L) Winter 2004 Instructor: Isabel Sumaya, Ph.D. Office: DDH H102 Office Hour: M: 2:00-4:00pm F: 11:00-12:50pm Email: isumaya@csub.edu Phone: 664-2381 Web site: www.csub.edu/~isumaya ___________________________________________________________________ TA: Mr. Patrick Grijalva Office Hours: TBA Email:TBA ___________________________________________________________________ Course Hours & Location: Collective Lab: M 4:30 – 5:20pm, DDH F100 Individual Lab Sections: MTWRF DDH H100 Recommended Texts: Lieberman, David A. (2000). Learning: Behavior and Cognition (3rd ed.). Belmont, CA.:Wadsworth/Thomson Learning. American Psychological Association. (2001). Publication Manual of the American Psychological Association (5th ed.). Washington DC: American Psychological Association. Prerequisites: Psyc 300 and 301 (or concurrent Psyc 301 enrollment) Course Description: This course focuses on classical and operant conditioning paradigms of animal learning. In this laboratory, you will have the unique opportunity to apply basic principles and concepts presented in the Principles of Learning course (Psyc 301) in a live learning organism, specifically, the laboratory rat. You will learn first hand the concept of shaping (method of successive approximation), schedules of reinforcements, extinction, and backward chaining of behavior. Course Structure: You will meet for one hour a day, five days per week. It is imperative that you arrive on time. Given the nature of the experimental paradigms, once the session begins for the day the door will be locked and you will not be allowed to participate for that session. Course Goals: 1. Use terminology appropriate to the field of animal learning (equipment identification, and procedures) 2. Understand the theoretical underpinnings of shaping, schedules of reinforcement, and extinction 3. Demonstrate proficiency in the handling and care of laboratory rats 4. Appreciate the use of animals and animal models in scientific research 5. Discuss the ability of animals to adapt to their environment through the phenomenon of learning 6. Analyze and interpret behavioral data and communicate findings (APA formatted papers & SPSS analysis) 7. Discuss the value of animal care and training Assignments and points: Attendance (50 @ 4 pts ea) Lab write-ups (2@50 pts. ea) Research Paper Skinner/Pavlov Paper Presentation Tests (2 @ 25 pts ea) Total Points 200 100 100 25 25 50 500 Laboratory Policies Attendance: For the next 10 weeks, you will be fully responsible for a living organism, a laboratory rat. Therefore, attendance in the lab is mandatory. You will be awarded 4 points for each lab attended. For each session that you miss (regardless of excuse), 8 points will be deducted. You are required to notify the lab (the TA or me via personal communication or a message on my machine) in advance of your absence. If you miss class without advance notification you will automatically lose 50 points. Theoretically, during an unknown absence, your animal goes without being fed. Your animal is your responsibility. There will be no room for error. Additionally, you are responsible for cleaning the operant chamber after every session; forgetting to do so will cost you 8 points. Note: It is important that your animal complete daily training and his weight be monitored to ensure optimal motivation to learn. Lab Write-Ups/APA paper: The lab write-ups are based on data generated in your experiments. These write-ups will include analysis on both an individual basis and a group basis. Prior to each write-up you will receive detailed instructions as to what is required in each paper. You will be required to keep complete and accurate recording of data. Data sheets will be provided. It will be your responsible for recording the data daily. All lab write-ups will adhere to the APA format. Tests: Two tests will be administered during the quarter. The tests will be based on material covered in lab lectures (standard equipment knowledge, weight maintenance) and the animal care and use testing module you will take on the web. Presentation: You are required to give a powerpoint presentation at the end of the quarter. This presentation will be a group effort (groups of 3 students). You can present data you collected (must include a method and results section) or you can present a presentation on Skinner or Pavlov. It will be your choice as to what to present. Additionally, regardless of what topic you choose you will be required to give a short synopsis of your experience in the lab at the end of your presentation. Lab Policies: Certain rules will be followed to ensure the safety of the animals and the students and the protection of lab equipment. 1. 2. 3. 4. 5. Attendance in the lab is required. You must arrive on time. Notify the instructor of any special circumstances that might affect your attendance. If you think you will have a problem with attendance, then drop the course. Tardiness and absences will negatively affect your grade. No food or drink is allowed in the lab. Protective clothing and the use of gloves is required for the handling of the animals. All research procedures must be adhered to carefully and with full attention to detail Things you may not do: --Mistreat the animals --Sabotage or otherwise damage the lab equipment or software --Falsify data Please note: Any student who participates in inhumane treatment of the animals, or purposely damages equipment, or intentionally reports falsified data, will be barred from the laboratory and receive a grade of F for the course. Schedule (subject to change) Week Topic 1 (1/05) Lab Lecture: Course Overview-Syllabus - Assignment: Animal Care & Use Training Module on Web (www.csub.edu/GradStudies/HCUAS) (Test #1 – Complete Module #1: Policies & Regulations and Module #2: The Rat) (Need to complete this by 1/09 by 12:00 noon by email) Lab: No lab for the week 2 (1/12) Lab Lecture: Magazine Training and Shaping - Assignment: Lab write-up #1 Body Weight Paper Lab: Equipment Orientation/Handling & Weighing Rats 3 (1/19) No Lab Lecture (Mr. King’s Day) No lab on Monday Lab: Magazine Training and Shaping of Rats 4 (1/26) Lab Lecture: Test #2: Equipment, Animal Handling & Body Weight Lab: CRF and FR Training 5 (2/02) Lab Lecture: Assignment: Lab write-up #2 - Shaping Paper Due: Lab write-up #1 Lab: FR Training 6 (2/09) Lab Lecture: Schedules of Reinforcement Lab: Schedules of Reinforcement 7 (2/16) No Lab Lecture Lab: Schedules of Reinforcement Due: Lab write-up #2 Final Paper - Schedules of Reinforcement Paper 8 (2/23) No Lab Lecture Lab: Extinction 9 (3/01) No lab Lecture Chained Operants – Due: Skinner/Pavlov Paper 10 (3/08) No Lab Lecture Lab: Chained Operants - 11 (3/15) Lab Lecture: Oral Presentations - Final Paper Due- Schedules of Reinforcement 11(3/17) Oral Presentations (Final time – Wed. 5:00pm – 7:30) California State University, Bakersfield Department of Psychology Principles of Learning Laboratory Manual Winter 2004 Welcome to the California State University, Bakersfield, Principles of Learning Laboratory vivarium (animal housing facility). This laboratory offers you the unique opportunity to experience animal research first-hand. The vivarium not only houses rodents for the use of the learning laboratory but also houses all on campus rodents involved in campus research. About this Manual This document is meant to serve as a working manual only and not as a final statement on the operation of the lab. With this in mind, the final word on any procedure or situation in the laboratory will be from the professor or the teaching assistant. About the Rats Your animal has been specifically bred for the laboratory setting. The purpose of breeding animals for the laboratory is to supply researchers with standardized animals in order to arrive at stable reproducible results in biological and behavioral experiments. Each of your rats has come from an identical source and has similar characteristics. In particular, each rat is an outbred (genetically heterogeneous) male Sprague-Dawley rat (Rattus norvegicus) purchased from the supplier, Harlan, Sprague Dawley, Inc., U.S.A. Each rat is Albino in coat color and is male. Initiated by R. Dawley, Sprague-Dawley Company, Madison, Wisconsin, in 1925, a hooded male (of unknown origin) was mated to a white female (of the Douredoure strain, probably from the Wistar) and subsequently to his white female offspring for seven successive generations. In 1980, Harlan purchased the former SpragueDawley company. It is important to note that the rats, through selective breeding during the first half of the 20th century, were specifically bred to be docile and easily handled by humans. Even still, it is still important to follow some guidelines when handling the animals. How to Handle the Animals Before handling a rat or placing your hand near the lid of the cage, remove the cage from the rack and place it on the counter. Then, while pressing against the front of the cage with your left hand, use your right hand to pull the metal clip off the cage top from underneath the plastic holders of the cage. You should now be able to lift the lid off of the cage. After you do this, place the lid to the side of the cage on the counter. Using your preferred hand, reach into the cage. With the tail of the rat toward the pinky finger region of your hand and the head toward the thumb/index finger region, place your hand on the back of the rat and gently curl your fingers around one side and your thumb around the other side of the rat. Your grip should not be so strong as to restrict breathing and potentially set off defensive behavior, yet should be strong enough so that you can support the weight of the rat. At this point, place the rat between your upper arm and your torso (underneath your arm pit) with the head toward the back of your arm. Rats prefer to be in a secure dark place as opposed to being out in the open. Once safely under your arm, you can now transport your animal to the outer room for placement in the operant chamber, or most likely, for placement in the tray for weighing. Follow the same procedures for removal from the chamber or the weigh tray and place the animal back into his home cage. Although you may have been taught that some animals can smell fear and react to it, what is more probably is that the animal will be more reluctant to interact with a hand that randomly approaches and retreats or is too quick and forceful. Therefore, as a general rule, it is good practice to be slow and deliberate when handling your animal being neither too forceful nor too apprehensive. Safety Attire On occasion, your rat will urinate or defecate during handling. In fact, you should expect this at some point during your time in the lab. With this in mind, you can either a) wear a protective covering (lab coat etc.,) during the handling of your rat or b) wear clothes to the lab that you are not concerned about soiling. Additionally, you will be required to wear gloves. This will protect you from the possibility of allergic reactions in the present and future. If you are bitten, first, do not panic. All of our rats are certified non-infected and are kept under very clean conditions while at CSUB. If the rat has not drawn blood, gently pick up the animal, return him to your cage, and inform your instructor. If the animal has drawn blood, return the animal to his cage and inform your instructor of the incident. At this point you will be directed to thoroughly wash the affected area with antibacterial soap and will be informed as to any additional actions to be take. Operant Conditioning Equipment There are two primary components to the operant conditioning equipment: the green outer chamber called the sound attenuating chamber (Coulbourn Instruments, U.S.A) which serves to reduce outer noise and offers greater control over the external environment and, the chamber itself (Coulbourn Instruments, Model # 84022SS). Each operant chamber is equipped with a food trough called the “magazine”, a bar/lever press, stimulus lights often referred to as cue lights (green, yellow & red), and a house light. Each chamber is also equipped with a pellet dispenser (the part with the clear plastic cylinder and lid) also known as the food hopper which is attached to the outer wall of the chamber. Additionally, each chamber is equipped with a hand switch serving as the manual control for the release of food pellets. Potential Problems with the Equipment Due to the nature of the equipment (particularly the pellet-delivery system or the hand switch), some types of problems may occur. For example, jams in the pellet dispenser can on occasion prevent the food reinforcer from falling into the food trough. Additionally, the hand switch may not work properly in triggering the triggering of the reinforcer. In both instances immediately notify the professor or the teaching assistant. Policy on Eating/Drinking in the Lab or Vivarium Because we will be working directly with electronic equipment, and because animals are present, food or drinks are not allowed in the lab. If you bring these items to class, please leave them outside the lab on the counter located just outside the lab. Animal Maintenance – Food The food the rats receive while in their home cages is a specially formulated diet which meets all the nutritional requirements for rodents, and is called maintenance rat chow or a standard lab diet (Purina Rat Chow, U.S.A). The pellet reinforcements are a more palatable version (Corn Syrup has been added) of the rats maintenance chow and is manufactured to be of consistent size, weight, and composition (Research Diets, Inc., New Brunswick, NJ. - Product# PJA/1-00045). Prior to the start of the laboratory the animals were maintained on an ad libitum diet. During your time in the lab, your rats will be maintained on 85% of its 100% body weight.