CLARK T. HUNG, P H .D.
10/13/2011
Department of Biomedical Engineering
351J Engineering Terrace MC8904
1210 Amsterdam Avenue
35 Sheffield Court
Ardsley, NY 10502
(914) 980-5550
New York, NY 10027
(212) 854-6542; fax (212) 854-8725 http://cellsrv.bme.columbia.edu; cth6@columbia.edu
_____________________________________________________________________________________
FIELD OF SPECIALIZATION
Physical effects on cells and musculoskeletal cell mechanotransduction and cartilage tissue engineering.
ACADEMIC TRAINING
University of Pennsylvania
P H .D., B IOENGINEERING , 12/95.
Dissertation: Real-Time Calcium Response to Fluid Flow in Cultured Bone Cells.
Advisor: Drs. Solomon R. Pollack and Carl T. Brighton
Philadelphia, PA
University of Pennsylvania
M.S.E., B IOENGINEERING , 5/92.
Advisor: Dr. John L. Williams
Philadelphia, PA
Brown University
S C .B., B IOENGINEERING, 5/90 .
Providence, RI
EMPLOYMENT RECORD AND UNIVERSITY SERVICE
1995
1996
Instructor, Department of Bioengineering, University of Pennsylvania, Philadelphia, PA (Fall)
Postdoctoral Fellow, Department of Bioengineering, University of Pennsylvania, Philadelphia,
PA (NASA microgravity-bioreactor studies- 1/96-6/96); Postdoctoral Fellow, Orthopaedic
Research Laboratory, Center for Biomedical Engineering, Columbia University, New York,
NY (cartilage research- 7/96-6/97)
1997-2002 Assistant Professor of Biomedical Engineering, Fu Foundation School of Engineering &
Applied Science (FFSEAS); Associate, Orthopaedic Research; Columbia University, New
York, NY (appointed 7/97)
1998- Director, Cellular Engineering Laboratory, FFSEAS
1999- Director, July summer high school course Physical Effects on Cells: Biomedical Engineering ,
Columbia Continuing Education and Special Programs, Columbia University, New York, NY
1999-2003 Department of Biomedical Engineering undergraduate and graduate committee member, sophomore class advisor, Biomedical Engineering Society (BMES) faculty advisor
2000-2004 Cellular Engineering Faculty Search Committee Member, Department of Biomedical
Engineering, FFSEAS
Clark T. Hung, Ph.D. 1.

1999-2004 Faculty advisor for Biomedical Engineering graduate students association (GraBME).
2002-2004 Associate Professor of Biomedical Engineering without tenure, FFSEAS
2004-2009 Associate Professor of Biomedical Engineering with tenure, FFSEAS (May)
2004- Member, Executive Committee, Department of Biomedical Engineering
2004-2007 Member, Nominating Committee, FFSEAS
2003-2005 Chair, Undergraduate Committee, Department of Biomedical Engineering, FFSEAS
2006- Member, Undergraduate Committee and Lab Committees, Department of Biomedical
Engineering, FFSEAS
2007- Member, BME Symposia Committee, Department of Biomedical Engineering, FFSEAS
2007- Research Coordinator, Research rotations for St. Lukes-Roosevelt Hospital orthopaedic surgery residents at Columbia BME labs.
2009- Professor, Department of Biomedical Engineering, FFSEAS
2010- Department of Biomedical Engineering sophomore class advisor, Biomedical Engineering
Society (BMES) faculty advisor, ABET committee (member), Lab Committee (chair)
2010- NIST summer fellowship faculty contact, Columbia University
HONORS/AWARDS
1990-95 NIH Bone & Cartilage Traineeship (Department of Orthopaedic Surgery, University of
Pennsylvania, Philadelphia, PA)
1996 Postdoctoral Fellowship (NASA- Wistar Institute-Bioengineering, University of Pennsylvania,
Philadelphia, PA)
1996
1997
2000
2002
2003
Solomon R. Pollack Award for Excellence in Graduate Bioengineering Research (Department of Bioengineering, University of Pennsylvania, Philadelphia, PA)
Whitaker Special Opportunity Award Postdoctoral Fellowship (Center for Biomedical
Engineering, Columbia University, New York, NY)
Guest co-editor, special Cell & Tissue Engineering issue of J Biomech Eng (June 2000).
Edward and Carole Kim Award for Faculty Involvement (Fu Foundation School of
Engineering & Applied Science, Columbia University, New York, NY)
NEGMA-LERADS Prize; 3 rd International Symposium on Mechanobiology of Cartilage and
Chondrocyte, Brussels, Belgium, May 16-17, 2003.
2004
2005
2006
2007
2008
Co-author, The John Paul Stapp Best Paper Award in the 2003 Stapp Car Crash Journal presented at the 47th Stapp Car Crash Conference. A tissue level tolerance criterion for living brain developed with an in vitro model of traumatic mechanical loading (Morrison III, B lead author)
Invited White Paper Author and Moderator: Biomechanics Curriculum Workshop at the
Whitaker Foundation Educational Summit II, Landsdowne, VA, March 3-6, 2005.
Invited Speaker: Tissue Engineering of Musculoskeletal Tissues Section, Gordon Research
Conference Musculoskeletal Biology & Bioengineering, Proctor Academy, Andover, NH, July
23-28, 2006.
Keynote Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage, 6 th
Annual Comparative Orthopaedics Research Day, College of Veterinary Medicine, University of Missouri, April 6 th , 2007.
Invited Symposium Plenary Speaker: The 21 st Century COE Program, International
Symposium Series on Mechanical Systems, The University of Tokyo, Japan, February 18.
Clark T. Hung, Ph.D. 2.

2008
2008
2009
2010
Keynote Speaker, Southeastern Meeting of the American Society of Biomechanics (SEASB),
Birmingham, AL, April 27-29.
Invited Speaker and Session Chair: Physical Regulation of Musculoskeletal Tissues Section,
Gordon Research Conference Musculoskeletal Biology & Bioengineering, Proctor Academy,
Andover, NH, July 28-August 1.
Elected Fellow, American Institute for Medical and Biological Engineering (AIMBE)
Elected Fellow, American Society of Mechanical Engineers (ASME)
PROFESSIONAL ACTIVITIES
Memberships
American Society of Mechanical Engineers (ASME): Bioengineering Division- Cell and Tissue
Engineering Committee, Biomedical Engineering Society (BMES), Orthopaedic Research Society (ORS),
International Cartilage Repair Society (ICRS), American Institute for Medical and Biological Engineering
(AIMBE)
Editorships
2006- Associate Editor, Journal of Biomechanical Engineering
2008- Editorial Board Member, The Open Orthopaedics Journal
2008- Deputy Editor, Journal of Orthopaedic Research
2009- Editor-in-Chief , Orthopaedic Research and Reviews (Dove Press open access journal)
2011-2014 Editorial Board Member, Tissue Engineering: Parts A, B, C
Ad-hoc Journal Reviewer
Clin Orthop Rel Res, Biophys J , J Biomechanics , J Orthop Res, J Biomech Eng, Arthritis Res , Arthritis
Rheum, Biochem Biophysica Acta, J Microscopy, Bone, J Bone Miner Res, Osteoarthritis Cartilage,
Calcified Tissue, Ann Biomed Eng, Tissue Eng, Acta Biomaterialia, Med Eng Physics, Stem Cells,
Langmuir, Bioeng Biotech, Proteomics, Biotech Prog, J Tissue Eng Regen Med, Nature Protocols,
Cartilage
1999 Session Organizer and Chair, ASME Summer Bioengineering Conference 1999 (Redmond,
MT): 6 Tissue Engineering sessions with Drs. Farshid Guilak and Ray Vanderby
1999 Session Organizer and Chair,1999 ASME Winter Meeting (Nashville, TN) 2 sessions on Cell
Mechanics
2000
2001
2001
2001
2001
2001
2002
Session Organizer and Chair, 2000 ASME Winter Meeting (Orlando, FL): a) Cell Mechanics ; b) Tissue Engineering
Session Moderator, Cartilage Mechanics , 47
Society (San Francisco, CA). th Annual Meeting of the Orthopaedic Research
Session Organizer and Chair, 2001 ASME Summer Bioengineering Conference (Snowbird,
Utah): a) Orthopaedic Tissue Engineering ; b) Cellular Engineering
Session Organizer and Chair for 2001 BMES Annual Meeting (Durham, NC):
Mechanotransduction III
Session Chair, 2001 ASME Winter Meeting (New York, NY): Tissue Engineering
Ad hoc Reviewer for Singapore Biomedical Research Council Grants (September)
Session Moderator: Cartilage Mechanics , 48 th Annual Meeting of the Orthopaedic Research
Society (Dallas, TX).
Clark T. Hung, Ph.D. 3.

2002
2003
2003
2003
Session Organizer, 2002 ASME Winter Meeting (New Orleans, LA): a) Cellular Engineering
& Mechanics ; b) Tissue Engineering
Session Organizer and Chair , 2003 ASME Summer Bioengineering Conference (Key
Biscayne, FL): Orthopaedic Cell & Tissue Engineering - 2 sessions.
Phone Reviewer for National Institute of Dental and Craniofacial Research (NIDCR) R03 grant applications, July 19.
Reviewer for CSR special emphasis panel ZRG1 SSS-U(40) for biomedical technology resource grant (NIH P41) at Tufts University (July 21-23).
Reviewer for abstracts to 50 th Annual meeting of the Orthopaedic Research Society 2003
2004
2004
Phone Reviewer for National Institute of Dental and Craniofacial Research (NIDCR) R03 grant applications, February 19.
Reviewer for CSR special emphasis panel ZRG1 BST-D (40) for biomedical technology resource grant (NIH P41) (Old Town, VA, April 7-8).
Mail Reviewer: for South Carolina NASA/EPSCoR Research Grant Program 2004
2004
2004
2004
2004
2004
2005
2005
2005
2005
Mail Reviewer: for CSR Skeletal Biology Development and Disease (SBDD) study section
R21 grant applications, June 27.
Co-Chair/Organizer: 3 sessions on Orthopaedic Mechanobiology at 2004 BMES Annual
Meeting (Philadelphia, PA)
Reviewer for abstracts to 51 st Annual meeting of the Orthopaedic Research Society
Mail Reviewer Consultant: Aircast Foundation
Mail Reviewer: Swiss National Science Foundation, Division of Biology and Medicine
Session Moderator: Molecular and Cellular Mechanics , 51 st
Orthopaedic Research Society (Washington, D.C.)
Annual meeting of the
Phone Reviewer: NIBIB (Marc Rigas, NIH/CSR); March 28, 2005.
Session Chair: 2005 Summer Bioengineering Conference, Vail, CO: Cell and Molecular
Engineering I & II, Cartilage Tissue Engineering
Phone Reviewer: Reviewer for CSR Telephone special emphasis panel (ZRG1 MOSS-K 04),
July 15
Reviewer for abstracts to 52 nd Annual meeting of the Orthopaedic Research Society 2005
2005
2007
Reviewer: NIH Cartilage Repair P01: December 6, 2005
Vice Chairman, Cell and Tissue Engineering Committee, Bioengineering Division of the
American Society for Mechanical Engineers
2006-2008 Chairman, Cell and Tissue Engineering Committee, Bioengineering Division of the American
Society for Mechanical Engineers
2006
2006
Session Organizer: Cellular Engineering/Biomechanics and Tissue Engineering/Biomechanics
(4 podium sessions, 2 poster sessions), 2006 Summer Bioengineering Conference, Amelia
Island, FL, June 22-26.
Session co-Chair: Tissue Engineering, 52 nd Annual meeting of the Orthopaedic Research
Society (Chicago, IL)
2006 Phone Reviewer: Special Emphasis Panel/Scientific Review Group 2006/05 ZRG1 MOSS-B
(03) (F) meeting, scheduled for 03/27/2006.
2006 Guest Reviewer: Aircast Foundation, Santa Monica, CA, May 17, 2006.
2006-2007 One Year Program Committee Member (Engineer), Orthopaedic Research Society
2006
2006
Reviewer for abstracts to 53rd Annual meeting of the Orthopaedic Research Society
Phone Reviewer: ZRG1 MOSS-L (04) Special Emphasis Panel, July 14, 2006.
Clark T. Hung, Ph.D. 4.

2006
2006
Session Organizer/Chair: Orthopaedic Tissue Engineering , 2006 Biomedical Engineering
Society Meeting, Chicago, October 11-14, 2006.
Session Co-Chair: Orthopaedic Soft Tissue Engineering , 2006 IEEE-EMBS Conference, New
York, NY, September 2006.
2006
2007
2007
2007
Phone Reviewer: Tissue Engineering and Regenerative Medicine ZAR1 CHW-M J2 1,
December 15, 2006.
Reviewer: Canada Research Chair nomination
Session Organizer: Cellular/Molecular Engineering and Tissue Engineering (4 podium sessions, 2 poster sessions), 2007 Summer Bioengineering Conference, Keystone, CO, June
20-24.
Phone Reviewer: Tissue Engineering and Regenerative Medicine ZAR1 CHW-J J1 1,
September 11, 2007.
Reviewer: Jeffress Research Grant from the Thomas F. & Kate Miller Jeffress Memorial Trust 2007
2007 Reviewer: South Carolina Experimental Program to Stimulate Competitive Research
(EPSCoR) and Institutional Development Awards (IDeA) programs
2007- Scientific Consultant: BREG, Inc., Vista, CA
2008
2008
External Reviewer, Hong Kong Research Grants Council
Ad hoc Reviewer, American Association for the Advancement of Science (K-INBRE)
2008 External Reviewer: Professorship in Animal Cell Biology at the University of Kuopio, Finland
2008 External Reviewer: The Technology Foundation STW- (Dutch Government)
2008 External Reviewer: Arthritis Research Campaign project grant application (United Kingdom)
2008 Reviewer, Biomaterials and Tissue Engineering Track Abstracts, 2008 Biomedical
2008
Engineering Society Meeting, St. Louis, MO
Reviewer, Abstracts to the 55 th Annual meeting of the Orthopaedic Research Society
2008-2011 Member, Executive Committee, ASME Bioengineering Division
2008
2008
2008
2009
2009
2009
2009
2010
2010
Reviewer, The Musculoskeletal Transplant Foundation grant applications
Ad hoc Reviewer: MTE Study Section, CSR, October 13, 2008.
Reviewer, Swiss Science Foundation
Session Organizer, Cell & Tissue Engineering, 2009 Summer Bioengineering Conference,
Lake Tahoe, CA, June 17-21.
Site Reviewer, on-site review of the Combat Casualty Care and Research Program,
Regenerative Medicine Research Program Area at the US Army Institute of Surgical research,
Fort Sam Houston, Texas, June 23-24, 2009.
Ad hoc Reviewer, NIH RC2 Go Grant Reviews, ZRG1 BCMB-B(99)July 30-31, 2009
Reviewer, Abstracts to the 56 th Annual Meeting of the Orthopaedic Research Society
Reviewer, 2010 Peer Review Orthopaedics Research Program (PRORP) for the Department of
Defense (DoD), January 24-26.
Session Organizer, Cartilage Repair/Regeneration and Osteochondral Grafts, 6th World
Congress of Biomechanics, Singapore, August 2010
2010
2010
2010
2010
2010
Ad hoc Reviewer: Reviewed grants for 2010 TERMIS EU Conference.
Abstract reviewer: Northeast Bioengineering Conference, Columbia University, New York
Ad hoc Reviewer: NIH ZRG1 F10B-3 20L (Fellowships), March 31.
Reviewer, The Musculoskeletal Transplant Foundation grant applications
Reviewer, Abstracts to the 57 th Annual Meeting of the Orthopaedic Research Society
Clark T. Hung, Ph.D. 5.

2010
2011
2011- Track Chair, Cellular and Molecular Bioengineering, BMES Annual Meeting, Hartford, CT,
October 12-15.
2011 Ad hoc Reviewer: NIH ZRG1 F10B (20) Study Section (Fellowships), March 15.
2011
2011
Phone Reviewer, California Institute for Regenerative Medicine: Basic Stem Cell Biology III
RFA, March 18.
Mail Reviewer: Collaborative Funding Grant (CFG), North Carolina Biotechnology Center,
April 5.
2011
Phone Reviewer, Panel member to review NIH/NIA Program Project on “Studies of Joint
Aging & Osteoarthritis”, October 28.
Guest Symposium Editor, Clinical Orthopaedics and Related Research (with Dr. James L.
Cook): Clinically Relevant Strategies for Cartilage Repair
Faculty Participant, Summer Bioengineering Conference Mentoring Workshop, Nemacolin
Resort, PA, June 24.
Ad hoc Reviewer: NIH ZRG1 F10B (20) Study Section (Fellowships), July 15. 2011
INVITED LECTURES
1.
Seminar Speaker: Real-Time Calcium Response to Fluid Flow in Cultured Bone Cells
Hospital for Special Surgery, New York, NY, August 12, 1995
; The
2.
3.
4.
Seminar Speaker: Real-Time Calcium Response to Fluid Flow in Cultured Bone Cells ; Columbia
University, Department of Mechanical Engineering, New York, NY, December 2, 1995
Seminar Speaker: Real-Time Calcium Response to Fluid Flow in Cultured Bone Cells ; Tulane
University, Department of Biomedical Engineering, New Orleans, LA, September 10, 1996
Seminar Speaker: Anterior Cruciate and Medial Collateral Ligament Fibroblasts Exhibit Different
Calcium Responses to Fluid Flow; Columbia University, Center for Biomedical Engineering, New
York, NY, January 23, 1997
5.
6.
Seminar Speaker: Chondrocyte Response to Fluid-induced Shear Stress ; Advanced Tissue
Sciences, LaJolla, CA, June 1997
Seminar Speaker: Real-Time Calcium Response to Fluid Flow in Cultured Bone Cells ; Indiana
Bone & Mineral Group, Indianapolis, IN, February 24,1999
7.
8.
Guest lecturer in BME 1001 Engineering in Medicine, Cartilage Tissue Engineering ; Department of Biomedical Engineering, New York, NY, February 28, 2000.
Seminar Speaker: Chondrocyte Response to Osmotic Loading: Experimental and Theoretical
Studies; University of Massachusetts Medical Center, Worcester, MA, January 29, 2001
9.
Invited Speaker: Functional Tissue Engineering of Articular Cartilage; University of Nancy,
France, April 23, 2001.
10.
Plenary Speaker: Functional Tissue Engineering of Articular Cartilage; EuroMech Colloquium
420: Mechanobiology of Cells and Tissues, Nancy, France, April 25,2001
11.
Invited Speaker: Determination of the Chondrocyte Environment within Cartilage Subjected to
Loading: Theoretical and Experimental Approach ; 2 nd International Symposium on
Mechanobiology of Chondrocytes and Cartilage, Paris, France, April 27, 2001.
12.
Invited Speaker: Cell & Tissue Culture Techniques: A Tutorial ; 2001 ASME Summer
Bioengineering Conference, Snowbird, UT, June 29, 2001.
13.
Invited Speaker: Functional Tissue Engineering of Articular Cartilage ; Columbia Class of 1951
Reunion, Arden House, Harriman, NY, September 8, 2001.
Clark T. Hung, Ph.D. 6.

14.
Invited Speaker: Fluid Flow Effects on Bone Cells: Influence of Flow-Cell-Substrate Interactions and Cell Mechanical Properties ; Fluid Flow in Bone Workshop, Phoenix, AZ, October 11, 2001.
15.
Invited Speaker: Fluid Flow Effects on Bone Cells: Influence of Flow-Cell-Substrate Interactions and Cell Mechanical Properties ; NYC Mineralized Tissue Seminar, New York, NY, December
12, 2001.
16.
Invited Speaker: Tutorial: Biomechanical Factors in Tissue Engineering: Bioreactors ; 48 th
Meeting of the Orthopaedic Research Society Meeting, Dallas, TX, February 10, 2002.
Annual
17.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage , Stanford
University Bio X Sponsored Symposium on Cartilage Tissue Engineering, Palo Alto, CA, March
22, 2002.
18.
Invited Speaker: Biomechanical Factors in Tissue Engineering: Bioreactors; Biomedical
Technology Track sponsored Bioreactor Design Tutorial at the 2002 Winter Annual ASME
Meeting, New Orleans, LA, November 20, 2002.
19.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage ,
Department of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, April 22,
2003.
20.
Invited Speaker: NEGMA-LERADS prize lecture: A Paradigm for Functional Tissue Engineering of Articular Cartilage , 3 rd International Symposium on Mechanobiology of Cartilage and
Chondrocyte, Brussels, Belgium, May 16-17, 2003.
21.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage using
Applied Deformational Loading , 4th International Conference on Tissue and Genetic Engineering,
Boston, MA, April 1-2, 2004.
22.
Invited Lecture: Engineering Biological Replacements for Joints , Columbia University Southern
California Dean’s Day Symposium, West Hollywood, CA, March 13, 2004.
23.
Invited Lecture : A Functional Tissue Engineering Strategy for Repair and Replacement of
Osteoarthritic Cartilage , European Society of Biomechanics 2004, the Netherlands, July 4-7,
2004.
24.
Invited Lecture : Chondrocyte Mechanotransduction: Cartilage Basic Science to Tissue
Engineering , Department of Biomedical Engineering, Purdue University, West Lafayette, IN,
September 24, 2004.
25.
Invited Lecture : Chondrocyte Mechanotransduction: Cartilage Basic Science to Tissue
Engineering , Brown University, May 5-6, 2005.
26.
Invited Speaker: Chondrocyte Mechanotransduction: Cartilage Basic Science to Tissue
Engineering, Society of In Vitro Biology Conference, June 4-7, 2005, Baltimore, MD
27.
Invited Speaker: Cartilage Biomechanics and Tissue Engineering , Stryker Medical, Mahwah, NJ,
July 22, 2005.
28.
Invited Speaker: Cartilage Tissue Engineering: Implications of Applied Deformational Loading and Solute Transport . McGowan Institute for Regenerative Medicine, University of Pittsburgh,
October 6, 2005.
29.
Invited Speaker: Cartilage Tissue Engineering: Implications of Applied Deformational Loading and Solute Transport . Department of Orthopaedic Surgery Grand Rounds and Department of
Biomedical Engineering Seminar Series, University of California-Irvine, December 1, 2005.
30.
Invited Speaker: Engineering Biological Tissues for Joint Replacement, Columbia University
Reunion Weekend “mini-course”, June 9, 2006.
31.
Invited Speaker: Physiologic Loading for Cartilage Tissue Engineering , Gordon Research
Conference Musculoskeletal Biology & Bioengineering, Proctor Academy, Andover, NH, July 23-
28, 2006.
Clark T. Hung, Ph.D. 7.

32.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage , NYU
Medical Center-Hospital for Joint Diseases, October 4, 2006.
33.
Invited Speaker: Chondrocyte Physical Regulation: Hierarchical Studies , Department of
Biomedical Engineering, SUNY Stony Brook, February 21, 2007.
34.
Keynote Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage, 6 th
Annual Comparative Orthopaedics Research Day, College of Veterinary Medicine, University of
Missouri, April 6 th , 2007.
35.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage , Upper
Extremity Rounds, Department of Orthopaedic Surgery, St. Lukes-Roosevelt Hospital, May 1,
2007.
36.
Invited Symposium Speaker: The 21 st Century COE Program, International Symposium Series on
Mechanical Systems, The University of Tokyo, February 18, 2008.
37.
Keynote Speaker, Southeastern Meeting of the American Society of Biomechanics (SEASB),
Birmingham, AL, April 27-29, 2008.
38.
Invited Speaker and Session Chair: Physical Regulation of Musculoskeletal Tissues Section,
Gordon Research Conference Musculoskeletal Biology & Bioengineering, Proctor Academy,
Andover, NH, July 28-August 1, 2008.
39.
Invited Speaker: Physiologic Loading for Strategies of Cartilage Repair , Hospital for Special
Surgery Research Division Seminar Series, October 10, 2008.
40.
Invited Speaker: Physiologic Loading for Strategies of Cartilage Repair, McGowan Institute of
Regenerative Medicine Scientific Retreat, Nemacolin Woodlands Resort, PA, March 9-11, 2009.
41.
Invited Speaker: Physiologic Loading for Strategies of Cartilage Repair , Department of
Bioengineering, University of Pennsylvania, April 23, 2009.
42.
Invited Speaker: Engineering New Knees- Are we close? Dean’s Day Mini-course, Columbia
University, Davis Auditorium, June 5, 2009
43.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage ,
Department of Biomedical Engineering, City College of New York, September 9, 2009
44.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage , Keck
Seminar Series, Gulf Coast Consortium, Rice University, Houston, TX, October 2, 2009.
45.
Invited Speaker, TMJ Bioengineering Conference, Boulder, CO, November 4-7, 2009
46.
Invited Speaker, A Paradigm for Functional Tissue Engineering of Articular Cartilage , Tissue
Engineering Monday Lunch Seminar Series, Irving Institute for clinical and translational research,
May 3, 2010.
47.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage , Upper
Extremity Rounds, Department of Orthopaedic Surgery, St. Lukes-Roosevelt Hospital, May 18,
2010.
48.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage ,
Department of Orthopaedic Surgery, University of Michigan, May 25, 2010.
49.
Invited Speaker: Strategies for Articular Cartilage Repair: Allograft Preservation & Tissue
Engineering, Department of Orthopaedic Surgery, Columbia University (Grand Rounds),
September 30, 2010.
50.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage . Society for Physical Regulation in Biology & Medicine, Miami Beach, FL. January 8, 2011
51.
Invited Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage , School of
Engineering, Brown University, March 7, 2011.
Clark T. Hung, Ph.D. 8.

52.
Invited Seminar Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage ,
Department of Mechanical Engineering, University of Colorado, Boulder, March 17, 2011.
53.
Invited Speaker,: Cell Based Joint Repair , SURF-NYSTEM Stem Cell Symposium, Columbia
University, August 4, 2011
54.
Invited Seminar Speaker: A Paradigm for Functional Tissue Engineering of Articular Cartilage ,
Department of Biological Sciences, University of Delaware, October 4, 2011.
55.
Invited Seminar Speaker: Strategies for Articular Cartilage Repair: Allograft Preservation &
Tissue Engineering, Department of Mechanical Engineering, The Cooper Union, November 15,
2011.
TEACHING RECORD
 Course Director: BMEN 1001 Engineering in Medicine- (Fall ’07, ’08, ’09,’11) freshman introductory course to BME- current enrollment 88 students
 Guest Lecturer: BIOL C2908 First-Year Seminar In Modern Biology- (Fall ’08, ’09, ’10)
 Co-Instructor: BIOL G4700 Seminar in Stem Cell Biology (Fall ’09, Spring ’10): graduate seminar course in stem cell biology offered as part of NYSTEM planning grant (with Chloe Bulinski and Dan
Kalderon, Department of Biological Sciences; Gordana Vunjak-Novakovic, BME)- enrollment 16-20 students
 Instructor: BMEN 4001,3001 Biomedical Engineering Principles I – (Fall ‘99,’00) quantitative molecular/cell biology- enrollment 40 students
 Instructor: BMEN 4002,3002 Biomedical Engineering Principles II – (Spring ‘98,‘99) quantitative physiology- enrollment 40 students
 Instructor: BMEN 4501 Tissue Engineering I (Fall ‘98, ‘99,’00, ‘01, ’02,’03, ‘04)- also on Columbia
Video Network (CVN) in ’04; 2007 Summer CVN course (3 students) (Average Course Quality:
4.12/5)
 Head Instructor or Module Instructor: BMEN 3820 Biomedical Engineering Laboratory II (Spring
‘01,‘02,’03,’04,’11; Fall ’06, ’07,’08, ’09, ’10 osmotic loading-cell hydraulic permeability module)- current enrollment 40 students
 Module Instructor: BMEN 3830 Biomedical Engineering Laboratory III (Fall ’99, Spring’06, Spring
’07, Spring ’08, ’09, ’11, Fall ‘11) fluid shear stress-cell adhesion module)- enrollment 40 students
 Instructor: BMEN 4502 Tissue Engineering II (Spring ’00,’06, ’07’,’08, ’09, ’10,’11) – current enrollment 38 students; also on Columbia Video Network (CVN) in Summer ’08 (1 student)
 Instructor: BMEN 3840 Biomedical Engineering Laboratory (Spring ‘99)- enrollment 40 students
 Independent Research Advisor: BMEN 3998 (Spring ‘00- present) – 1-3 students/semester
 Group Advisor: BMEN 3910-3920 Senior Design (Spring ‘99- present): 5 students- Advisor for projects aimed at chamber for osmolarity studies (‘98), a methodology to make alginate slabs (‘99); device to measure direct hydraulic permeability of soft hydrated tissues (‘00), alginate hollow microspheres (‘01,’02,’03). For Spring ’04: in vitro cartilage repair (4 students) model, pressure/deformation bioreactor design (4 students) and sperm motility projects (4 students). For ‘06-
’07: design of a controller for a portable drug delivery pump for blind diabetic patients (Infuze group).
For ’07-’08, advisor for group developing rehabilitation EMG-online monitor for knee injuries (5 students). For ’08-09, advisor for group looking at product for incontinence (5 students); For ’09-’10, advising 2 groups of 4 students each- a) device to reduce hand tremors in MS and Parkinson’s patients
Clark T. Hung, Ph.D. 9.

and b) device to aid cervical dilation. For ’10-’11 advising 1 group of 4 students on design of newborn baby carrier for transport to the hospital in third world communities.
 Co-Instructor: G3200 Cellular Sensing, Transduction, and Amplification of Mechanical Forces
(Spring ’05; City College of New York biomedical engineering course directed by Professor Sheldon
Weinbaum): instructor for module on cartilage mechanotransduction (10 students).
 Module Instructor: BMEN 6001 Advanced Quantitative Physiology; Spring ’07, ‘08: cell migration and attachment 3-week module including wet lab, 24 graduate students.
 Director/Head Instructor: Summer BME High School Course: Physical Effects on Cells (July 99- present)- total of 42 high school students per year (two parallel sections). Course developed and administered through the Columbia High School Programs, Columbia Continuing Education and
Special Programs. The month long course provides an introduction to the multidisciplinary field of biomedical engineering. International students have also participated in this course. Teaching assistants are BME undergraduates.
FELLOWS/STUDENTS/ STAFF SUPERVISED
Postdoctora Fellowsl-Visiting Scholars: present position
1.
Glyn D. Palmer, Ph.D. (9/99-12/00): Research Associate at NYU-Hospital for Joint Disease, New
York, NY
2.
Michelle A. LeRoux-Williams, Ph.D. (9/00-10/01): Chief Scientific Officer at Osiris Therapeutics,
Baltimore, MD
3.
Christopher C-B. Wang, Ph.D. (8/01-6/03 ): Director, Open Underwriting- Pricing and Balance
Transfer, American Express, New York, NY
4.
Terri-Ann N. Kelly, Ph.D. (6/06-12/06): Completed postdoctoral fellowship at the Department of
Physics, Georgetown University, Washington D.C.
5.
Erik Finkelstein, Ph.D. (5/07-8/07, Department of Biological Sciences under Dr. Chloe Bulinski):
Department of Health in the City of New York, New York
6.
Kenneth W. Ng, Ph.D. (10/07-8/08): Assistant Medical Director in the scientific services group of
Health Science Communications
7.
Eric G. Lima, Ph.D. (5/08-8/31/08): Assistant Professor of Mechanical Engineering, Cooper Union
University, New York, NY.
8.
Jason Fong, M.D. (3/09-3/10): Postdoctoral fellow at Stevens Institute of Technology, NJ
9.
Najmuddin Gunja, Ph.D. (2/09-6/10): Postdoctoral fellow at Polytechnique Montreal, Canada
10.
Grace O’Connell, Ph.D. (10/09-): Postdoctoral fellow (from University of Pennsylvania)
11.
Elena Alegre Aguaron, Ph.D. (8/10-): Visiting scholar (from University of Zaragoza, Spain)
12.
Silvia Panseri, Ph.D. (3/11-9/11): Visiting scholar (from University of Bologna, Italy)
Doctoral Students: dissertation title
1.
Co-Advisor: Christopher C-B. Wang (7/99- 8/01): Ph.D. thesis in Mechanical Engineering:
Functional properties of cartilage and environment of chondrocytes: experiments and theoretical analyses (currently at American Express, New York, NY)
2.
Co-Advisor: Robert L. Mauck (8/98-7/29/03): Ph.D. thesis in Biomedical Engineering: Functional tissue engineering of articular cartilage: The effect of physical forces on the in vitro growth of engineered constructs- Whitaker Foundation graduate fellowship (currently Assistant Professor of
Orthopedics and Bioengineering (tenured), University of Pennsylvania, Philadelphia, PA)
Clark T. Hung, Ph.D. 10.

3.
Advisor: Pen-Hsiu Grace Chao (1/99-8/05 ): Ph.D. thesis in Biomedical Engineering: Physical forces on cells: effects of applied osmotic loading and direct current electric fields (currently Assistant
Professor, Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan)
4.
Advisor: Terri-Ann N. Kelly (1/00-5/06): Ph.D. thesis in Biomedical Engineering: Functional tissue engineering of articular cartilage: characterization and optimization of chondrocyte-seeded agarose hydrogels- NIH minority supplemental graduate fellowship (currently at the Department of Physics,
Georgetown University-NIH for a postdoctoral fellowship)
5.
Co-Advisor: Nadeen O. Chahine (9/00-5/06): Ph.D. thesis in Biomedical Engineering: Multi-scale measurements of the mechanical and transport properties of native and engineered articular cartilage
(Assistant Investigator, Feinstein Institute for Medical Research, Manhasset, NY)
6.
Advisor: Kenneth W. Ng (9/01-2/08): BME doctoral candidate: Recreating biomimetic micro-scale and macro-scale structure in engineered articular cartilage. Doctorate with Honors. Awardee of the
Hung-Huang Graduate Award in Biomedical Engineering (Postdoctoral Researcher, Hospital for
Special Surgery, NY starting 9/08-6/11)).
7.
Co-Advisor: Eric G. Lima (9/01-5/08): BME doctoral candidate: Tissue engineering of cartilage: transitioning from the lab towards a clinical setting (Assistant Professor, Mechanical Engineering,
Cooper Union University, New York, NY)
8.
Advisor: Elizabeth S. Oswald (9/04-9/10): BME doctoral candidate (NSF-Presidential Graduate
Fellowship): Effects of extracellular osmotic environment on chondrocyte and precursor cell physical and metabolic properties . (Whitaker Postdoctoral International Fellow to Taiwan)
9.
Advisor: Liming Bian (1/05-8/09): BME doctoral candidate: Articular cartilage repair with two in vitro approaches: engineered cartilage and cartilage allograft ( Postdoctoral Researcher, University of
Pennsylvania, PA)
10.
Advisor: Andrea Tan (9/07-): BME doctoral candidate (NSF Graduate Fellowship)
11.
Advisor: Sonal Sampat (9/09)- BME doctoral candidate
12.
Co-Advisor: Adam Nover (7/10-)- BME doctoral candidate
13.
Advisor: Brendan Roach (8/11-)- BME doctoral candidate
Master’s Students: present position
1.
Louis Cappezuto, J.D. (1/02-5/02): M.S., Biotechnology Program (consul at Johnson and Johnson)
2.
Amir Hakakha, M.S.E. (1/01-9/01): Ortec, Manager of Technology Development, New York, NY
3.
HyukJin Lee, M.S.E. (8/03-5/04): Went back to Korea
4.
Shelley Han, M.S.E., (1/02-7/04): UCLA Medical School
5.
Patricia B. Setti, M.S.E., (9/04-12/04): Stryker Orthopaedics (Regulatory Affairs), Mahwah, NJ
6.
Mandy Ho (9/02-9/06): American Express, New York, NY
7.
Andrew Chen (1/09-5/10): Stryker, San Jose, CA
8.
Divya Dujari (1/10-12/10)- consulting position (ZS Associates) in San Francisco, CA
9.
Evelyn Chen (9/10-12/10)- industry position in Taipei, Taiwan
10.
Ben Rho (9/09-1/11)- Harvard law school
11.
Nikhil Joshi (9/10-2/11)- industry
12.
Katherine Wood (9/11-)
Undergraduate Students: present position
1.
Rani Roy, FFSEAS ’00: Ph.D., Cornell University BME
2.
Sarah Desoky, FFSEAS ’00: uncertain
Clark T. Hung, Ph.D. 11.

3.
Lia Hondroulis, FFSEAS ’00: Andrx Pharmaceuticals (candidate for law school)
4.
Wendy Liu (MIT ‘00, summer 1999): Ph.D. from University of Pennsylvania BME
5.
Dennis Wong, FF SEAS ’01: Working at the National Security Agency
6.
Brian Kim, FF SEAS ’01: Premed coursework at Columbia
7.
Aparna Prasad, FF SEAS '01: Medical student at SUNY Buffalo Medical School
8.
Nelly Andarawis, FF SEAS '01: Ph.D., University of Pennsylvania BME
9.
Kelly Jamieson, FF SEAS ’02: Research Technician at Mt. Sinai Medical Center
10.
Laura Rummler, FF SEAS ’02: Research engineer at Peripheral Nerve Research Laboratory, UC
Irvine; Doctoral candidate UC Irvine
11.
Nicole Gabriel (Rice University ’03, summer 2002): Ph.D. candidate at University of Pennsylvania
BME
12.
Elizabeth Oswald, FF SEAS '04: Columbia University BME, PhD in BME (Columbia(
13.
QiQi Cheng, FF SEAS '04: Ph.D. candidate at University of Pennsylvania BME
14.
Christopher DuBois, FF SEAS '04: CUIT, Columbia University
15.
Selom Gasinu, FF SEAS '04: Attending Cornell Medical School
16.
Michael Bazylewicz, FF SEAS ’04 (summer 2004): Attending Dartmouth College Medical School
17.
Mathew Fisher, FFSEAS’05: University of Pittsburgh BME as a doctoral candidate
18.
Lauren Statman, FFSEAS ’05: UCSF Bioengineering- NIH NIAMS doctoral candidate
19.
Anne-Marie Cannon (Rice University ’05, summer 2003): employed at
Xenogen Biosciences, in
Cranbury NJ
20.
Fran Navratil, FFSEAS ’04: MTV, New York, NY
21.
Jesse Martin, FFSEAS’05, Research Assistant, Columbia Medical Center
22.
Timon Tai, FFSEAS ’06, Master’s of Public Health at Columbia University
23.
Gilad Landan FFSEAS ’06- uncertain
24.
Whitney Booker, FFSEAS ’05, clinical research associate in Department of Orthopaedic Surgery
(Pediatrics) at Columbia University
25.
Lindsay Kugler, FFSEAS ’08- Medical School
26.
Evan Lin, FFSEAS ’07- uncertain
27.
Stephanie Angione (Brown University ’08, summer student)- doctoral student at Brown University
28.
Chris O’Conor, FFSEAS ’08, SURF summer 2007, Amgen Scholar; MD-PhD at UNC-Chapel Hill
29.
Alex Dinh, FFSEAS ’10- Health consulting firm
30.
Pojen Deng, FFSEAS ’07, 2007 summer, Candidate for M.D., Brown University
31.
David Williams, FFSEAS ’09- Master’s student at Duke University
32.
David Mao, FFSEAS ’09- uncertain
33.
Duo Xu, FFSEAS ’10- NYU laboratory research assistant
34.
Kimberly Lipman-White, FFSEAS ‘11
35.
Sarah Kramer, FFSEAS ’10- Health consulting firm in Washington, DC
36.
Heidi Ahmed, FFSEAS ’11- Medical school at University of Iowa
37.
Alyson Cunningham, FFSEAS ‘12
38.
Man-Yu Moy, CC ‘11
39.
Elizabeth Dong, CC’10- medical school at NYU starting 8/11
40.
Alba Luengo, FFSEAS ‘12
41.
Adam Nover, FFSEAS ’10- BME Doctoral candidate at Columbia University
Clark T. Hung, Ph.D. 12.

42.
Isabella Newman, Brown University ’12 (summer 2010, 2011)
43.
Michael Carapezza, FFSEAS ’13 (Amgen Fellow, summer 2011)
44.
Amy Mei, Brown University ’13 (NYSTEM Fellow, summer 2011)
45.
Mary Ye, FFSEAS ’12 (summer 2011)
46.
Matt Dermksian, Bates College ’14 (summer 2011)
47.
Reuben Saunders (CC-Biology)
48.
Drew Robinson , FFSEAS’15
49.
Jennifer Xiong, FFSEAS’15
50.
Joya Ahmad, FFSEAS’15
Medical Students: present position
1.
Frank Raia, M.D.: Completed orthopaedic residency at Columbia-Presbyterian
2.
Ross Henshaw, M.D.: Completed orthopaedic residency at Columbia-Presbyterian
3.
Sansan S. Lo, M.D.: (9/00-5/01): Completed medical school training at Columbia University
4.
Jenny Yuan (summer 2007): M.D., Ph.D. candidate at Columbia University
Orthopaedic Residents
1.
Justin Saliman, M.D.: (1/04-3/04) Orthopaedic resident at St. Luke’s-Roosevelt
2.
David Capiola, M.D.: (3/04-5/04) Orthopaedic resident at St. Luke’s-Roosevelt
3.
Steve Quinnan, M.D.: (1/05-3/05 Orthopaedic resident at St. Luke’s-Roosevelt
4.
Darren Friedman, M.D.: (3/05-5/05) Orthopaedic resident at St. Luke’s-Roosevelt
5.
Jason DeFrancis, M.D.: (1/06-3/06) Orthopaedic resident at St. Luke’s-Roosevelt
6.
Francis Gonzales, M.D.: (1/07-3/07) Orthopaedic resident at St. Luke’s-Roosevelt
7.
Damien Davis, M.D.: (3/07-5/07) Orthopaedic resident at St. Luke’s-Roosevelt
8.
Jennifer Sohal, M.D.: (5/07-7/07) Orthopaedic resident at St. Luke’s-Roosevelt
9.
Don Heitman, M.D.: (1/08-3/08) Orthopaedic resident at St. Luke’s-Roosevelt
10.
Keith Crivello, M.D.: (3/08-5/08) Orthopaedic resident at St. Luke’s-Roosevelt
11.
James Barsi, M.D.:(5/08-7/08) Orthopaedic resident at St. Luke’s-Roosevelt
12.
Michael Alapatt, M.D.: (1/09-3/09) Orthopaedic resident at St. Luke’s Roosevelt
13.
Doug Scott, M.D.,: (3/09-5/09) Orthopaedic resident at St. Luke’s Roosevelt
14.
Vijay Borra, M.D.: (5/09-7/09) Orthopaedic resident at St. Luke’s Roosevelt
15.
Avrum Joffe, M.D.: (1/10-3/10) Orthopaedic resident at St. Luke’s Roosevelt
16.
James Andry, M.D.: (3/10-5/10) Orthopaedic resident at St. Luke’s Roosevelt
17.
Ryan Dowling, M.D.: (5/10-7/10) Orthopaedic resident at St. Luke’s Roosevelt
18.
Neil Dunleavy, M.D.: (1/11-3/11) Orthopaedic resident at St. Luke’s Roosevelt
19.
Curtis Vandenburg, M.D.: (3/11-5/11) Orthopaedic resident at St. Luke’s Roosevelt
20.
George Ackerman, M.D.: (5/11-7/11) Orthopaedic resident at St. Luke’s Roosevelt
Staff Positions: present position
1.
Sara Seyhan, B.S.E.: (2/00-2/02) Orthosonics LTD, OSCAR product specialist
2.
Eric G. Lima, Ph.D.: (9/1/08-) Part-time Adjunct Associate Research Scientist, Department of
Biomedical Engineering, Columbia University
DISSERTATION COMMITTEES
Clark T. Hung, Ph.D. 13.

1.
Michael Huang (MSE,‘98): Development of an apparatus and technique to measure calcium binding in skeletal and cardiac muscle (examiner)
2.
Andrey Levchenko (DES, ’98, Biomed Eng): Expression and action of P-glycoprotein: A quantitative analysis (examiner)
3.
Hong An (Ph.D., ’99; Chem Eng): Cell deformation in an asymmetric thin liquid film (examiner)
4.
Shawn Gomez (EngScD ,’99, Biomed Eng ): Attachment of cells to surfaces under controlled fluid flows (examiner)
5.
Sarah Patrick (Ph.D., ’99, Chem Eng): Control of receptor-ligand contact to examine the kinetics of T cell activation (examiner)
6.
Michael Soltz (Ph.D., ’00; Mech Eng): Investigation of a boundary friction model for articular cartilage: Effects of interstitial fluid pressurization and surface topography (examiner)
7.
Chun-Yuh Huang (Ph.D., ’01, Mech Eng): Biomechanics of soft tissues in the shoulder jointglenohumeral cartilage, ligament, and rotator cuff tendon (examiner)
8.
Sobin Kim (Ph.D., ’01; Chem Eng): Kinetics of calcium mobilization of a T cell activated by controlled receptor-ligand contact (examiner)
9.
Vincent Wang (Ph.D., ’01; Mech Eng): Biomechanics of the normal and surgically reconstructed shoulder (examiner)
10.
Stavroula Sofou (Ph.D., ’01; Chem Eng): Control of phospholipid vesicle organization with cysteinecontaining oligopeptides (examiner)
11.
Changbin Wang (Ph.D., ’01; Mech Eng): Digital video microscopy-based determination of cartilage inhomogeneity, anisotropy and tension-compression nonlinearity: Implications on chondrocyte environment (co-sponsor)
12.
Chi Hyun Kim (EngScD,’03; Mech Eng): In vivo trabecular bone response to mechanical loading and parathyroid hormone stimulation (chairman, examiner)
13.
Zhongliang Tang (Ph.D., ’03; Chem Eng): Microfluidics: fluid flow, transport, control, characterization and applications (examiner)
14.
Robert Mauck (Ph.D., ’03, Biomed Eng): Functional tissue engineering of articular cartilage: The effect of physical forces on the in vitro growth of engineered constructs (co-sponsor)
15.
Vedran Knezevic: Doctoral proposal: A system for biaxial loading of fibroblast-populated collagen gels for the study of mechanobiology (examiner)
16.
Pen-Hsiu Grace Chao (Ph.D., ’05, Biomed Eng): Physical forces on cells: effects of applied osmotic loading and direct current electric fields (sponsor)
17.
Mia Mia Thi (City College of New York): Doctoral proposal: Effects of fluid shear stress on bone cell gap junctional communication (examiner)
18.
Patrick Johnson (Ph.D., ’04; Chem Eng): Polymer-anchored DNA monolayers for electrochemical sensing (examiner)
19.
Peng Wang (Ph.D., ’04; Chem Eng): Polymeric thin films: preparations, surface modification and morphology (examiner)
20.
Erica Takai (Ph.D., ’05, Biomed Eng): Modulation of Bone Cell Mechanotransduction (examiner)
21.
Seonghun Park (Ph.D., ’05, Mech Eng)): Doctoral pre-proposal and proposal: Interstitial fluid flowdependent and flow-independent mechanical and tribological response of articular cartilage (Mech
Eng) (examiner)
22.
Nadeen Chahine (Ph.D., ’06, Biomed Eng): Multi-scale measurements of the mechanical and transport properties of native and engineered articular cartilage (co-sponsor)
23.
Terri-Ann Kelly: (Ph.D, ’06, Biomed Eng): Characterization and optimization of chondrocyte-seeded agarose constructs grown in free-swelling and dynamic- loading culture (sponsor)
Clark T. Hung, Ph.D. 14.

24.
Alice Eun Jun Lee (Ph.D., ’06, Biomed Eng): Structural and functional characterization of engineered cardiac tissue (chair)
25.
Thomas Gardner: (Biomed Eng): Doctoral proposal: The effect of hormone replacement therapy on the biomechanics of the simian glenohumeral joint (examiner)
26.
Jie Jiang (Ph.D. candidate, Biomed Eng): Doctoral proposal: The role of cell-to-cell interactions in chondrocyte calcification and maintenance of the osteochondral interface (examiner)
27.
Kenneth Ng (Ph.D., ‘08, Biomed Eng): Recreating biomimetic micro-scale and macro-scale structure in engineered articular cartilage (sponsor)
28.
Eric Lima (Ph.D., ’08, Biomed Eng): The tissue engineering of functional anatomically-shaped osteochondral constructs for cartilage repair (sponsor)
29.
Johnathan Lai (Ph.D., ’07 Biomed Eng): The potential mechanistic interactions of G3139 (Genasense) in prostate cancer and melanoma cells (chair)
30.
Dae Kim (Ph.D., ’08 Biomed Eng): Four-Color DNA Sequencing by Synthesis on a Chip Using
Cleavable Fluorescent Nucleotide Reversible Terminators (examiner)
31.
Elaine Wang (M.S., Biomed Eng): Role of heterotypic cellular interactions in the regeneration of the anterior cruciate ligament-to-bone interface (chair)
32.
Clare Canal (Ph.D., ’08, Biomed Eng): Contact mechanics of articular layers: 2D strain fields and their relation to tissue anisotropy and inhomogeneity (chair)
33.
Do Eun Kim (Ph.D., ’08, Biomed Eng): Enhancing contractile function in healthy and injured engineered cardiac tissues (chair).
34.
Nina Tandon (Ph.D., ‘09, Biomed Eng): Biomimetic electrical stimulation for cardiac tissue engineering (chair)
35.
Tim Martens (M.D., Columbia Medical School, Ph.D., ’09, Biomed Eng): Cell delivery platforms for cardiovascular repair (chair)
36.
Evren U. Azeloglu (Ph.D., ’08 Biomed Eng): Biomechanical hierarchy of structural heterogeneity in cardiovascular tissues (examiner)
37.
Elizabeth S. Oswald (Ph.D. ‘10, Biomed Eng): Effects of extracellular osmotic environment on chondrocyte and precursor cell physical and metabolic properties (sponsor, defense)
38.
Liming Bian (Ph.D. candidate, Biomed Eng): Articular cartilage repair with two in vitro approaches: engineered cartilage and cartilage allograft ( sponsor)
39.
Keyue Shen (Ph.D., ’09, Biomed Eng): Micropattern- and rigidity-modulated T cell activation (chair)
40.
Peng Shi (PhD., ’09, Biomed Eng): Multicomponent interfaces modulate neural development in vitro
(chair)
41.
Caroline Maleke (PhD, ’09, Biomed Eng): In vivo detection and thermal treatment monitoring of breast tumors using harmonic motion imaging (HMI) (chair)
42.
Michael Albro (PhD, ’09, Biomed Eng): Solute transport in porous deformable media: active uptake in dynamically loaded tissue and molecular partitioning in the cellular cytoplasm (chair).
43.
Jones Tsai (PhD, ’10, Biomed Eng): Probing Cell-Signaling Crosstalk through Micro- and Nano-
Surface Engineering (chair)
44.
Matteo Caligaris (PhD, ’10, Mech Eng): Interstitial fluid pressurization dominates the frictional response of healthy and arthritic cartilage (examiner)
45.
Robert Maidhof (PhD, ’10, BME): Simultaneous culture medium perfusion and electrical stimulation improve differentiation of engineered cardiac tissue (chair)
46.
Kristin Moffat (PhD, ’10 BME): Biomimetic nanofiber scaffold design for tendon-to-bone interface tissue engineering (chair)
Clark T. Hung, Ph.D. 15.

47.
Brian Gillette (PhD, ’10, Biomed Eng): Microfabrication of collagen-based extracellular matrices for tissue engineering (chair)
48.
Shelby Zhang (PhD, ’11 BME): Modulation of cell proliferation and differentiation (committee chair)
49.
James J. Kwan (PhD, ’11 Chem Eng): Phospholipid encapsulation properties and effects on microbubble stability and dynamics (examiner)
50.
Andrew Baik (PhD, candidate BME): Application of a Quasi-3D Imaging Technique to Characterize
Early Mechanotransduction Events in Osteocytes (examiner)
51.
Andrea Tan (PhD, candidate BME): Role of cell migration and mechano-chemical preconditioning in the reparative response of tissue engineered cartilage to mechanical injury and inflammatory cues
(advisor).
JOURNAL ARTICLES
1.
Hung CT, Williams JL: A method for inducing equi-biaxial and uniform strains in elastomeric membranes used as cell substrates. J Biomechanics 27(2):227-232, 1994.
2.
Hung CT, Pollack SR, Reilly TM, Brighton CT: Real-time calcium response of cultured bone cells to fluid flow. Clin Orthop Rel Res 313:256-269, 1995.
3.
Hung CT, Allen FD, Pollack SR, Brighton CT: What is the role of the convection current density in the real-time calcium response of cultured bone cells to fluid flow? J Biomechanics 29(11):1403-
1409, 1996.
4.
Hung CT, Allen FD, Pollack SR, Brighton CT: Intracellular Ca 2+ stores and extracellular Ca 2+ are required in the [Ca 2+ ] i
response of bone cells experiencing fluid flow. J Biomechanics 29(11):1411-
1417, 1996.
5.
Allen FD, Hung CT, Pollack SR, Brighton CT: Comparison of the [Ca 2+ ] i
response to fluid flow of
MC3T3-E1, ROS 17/2.8, and cultured primary osteoblast-like cells. Cell Eng 1:117-124, 1996.
6.
Hung CT, Allen FD, Mansfield K, Shapiro IR: Extracellular ATP modulates [Ca 2+ ] i treated embryonic chondrocytes. Am J Physiol 272 (5 Pt 1): C1611-C1617, 1997.
in retinoic acid
7.
Hung CT, Allen FD, Pollack SR, Attia ET, Hannafin JA, Torzilli PA: Anterior cruciate and medial collateral ligament fibroblasts exhibit different Ca 2+ responses to fluid flow. Cell Signal 9(8):587-
594, 1997.
8.
Mow VC, Wang, CC, Hung CT: The extracellular matrix, interstitial fluid and ions as a mechanical signal transducer in articular cartilage. Osteoarthritis Cartilage 7:41-59, 1999.
9.
Hung CT, Henshaw DR, Wang CC-B, Mauck R, Raia F, Palmer G, Mow VC, Ratcliffe A, Valhmu
WB: Mitogen-activated protein kinase signaling in bovine articular chondrocytes in response to fluid flow does not require calcium mobilization. J Biomechanics 33:73-80, 2000.
10.
Chao P-HG, Roy R, Mauck RL, Liu W, Valhmu WB, Hung CT: Chondrocyte translocation response to direct current electric fields. J Biomech. Eng .
122:261-267, 2000.
11.
Mauck RL, Soltz MA, Wang CC-B, Wong DD, Chao P-HG, Valhmu WB, Hung, CT, Ateshian GA:
Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. J Biomech Eng 122:252-260, 2000.
12.
Allen FD, Hung CT, Pollack SR, Brighton CT: Mechano-chemical coupling in the flow-induced activation of intracellular calcium signaling in primary cultured bone cells. J Biomechanics
33(12):1585-1591, 2000.
13.
Wang CC-B, Hung CT, Mow VC: Inhomogeneity of aggregate modulus affects cartilage compressive stress-relaxation behavior. J Biomechanics 34(1): 75-84, 2001.
Clark T. Hung, Ph.D. 16.

14.
Palmer GD, Chao P-HG, Raia F, Mauck RL, Valhmu WB, Hung CT: Time dependent aggrecan gene expression of articular chondrocytes subjected to hyperosmotic loading. Osteoarthritis Cartilage 9(8):
761-770, 2001.
15.
Wang CC-B, Guo, XE, Sun, D, Mow VC, Ateshian GA, Hung CT: The functional environment of chondrocytes within cartilage subjected to compressive loading: theoretical and experimental approach. Biorheology, 39(1-2):39-45, 2002.
16.
Wang CC-B, Ateshian GA, Hung CT: An automated approach for direct measurement of strain distributions within articular cartilage under unconfined compression. J Biomech Eng 124:557-
567,2002.
17.
Mauck RL, Seyhan SL, Ateshian GA, Hung CT: The influence of seeding density and dynamic deformational loading on the developing structure/function relationships of chondrocyte-seeded agarose hydrogels. Ann Biomed Eng 30:1046-1056, 2002.
18.
Chafik D, Bear D, Bui P, Patel A, Jones NF, Kim BT, Hung CT, Gupta R, Optimization of schwann cell adhesion in response to shear stress in an in vitro model for peripheral nerve tissue engineering.
Tissue Eng 9(2):233-241, 2003.
19.
Ateshian GA, Soltz MA, Mauck RL, Hung CT, Lai WM: The role of osmotic pressure in the frictional response of articular cartilage. Transport Porous Media 50:5-33,2003.
20.
Hung CT, LeRoux MA. Palmer GD, Chao PG, Valhmu WB: Disparate aggrecan gene expression in chondrocytes subjected to hypotonic and hypertonic loading in 2D and 3D culture.
Biorheology 40(1-
3): 61-72, 2003.
21.
Wang CCB, Chahine NO, Hung CT, Ateshian GA: Optical determination of anisotropic material properties of bovine articular cartilage in compression, J Biomechanics 36(3): 339-353, 2003.
22.
Mauck RL, Nicoll SB, Seyhan SL, Ateshian GA, Hung CT: Synergistic effects of growth factors and dynamic loading for cartilage tissue engineering. Tissue Eng 9(4), 597-611, 2003.
23.
Mauck RL, Hung CT, Ateshian GA: Modeling of neutral solute transport in a dynamically loaded porous permeable gel. J Biomech Eng 125(5): 602-614, 2003.
24.
Hung CT, Lima EG, Mauck RL, Takai E, LeRoux MA, Lu HH, Stark RG, Guo XE, Ateshian GA:
Anatomically shaped osteochondral constructs for articular cartilage repair. J Biomechanics 36(12):
1853-1864, 2003.
25.
Mauck RL, Wang CC-B, Oswald ES, Ateshian GA, Hung CT: The role of cell seeding density and nutrient supply for articular cartilage tissue engineering with deformational loading Osteoarthritis
Cartilage 11(12): 879-890, 2003.
26.
Morrison III B, Cater HL, Wang CC-B, Thomas FC, Hung CT, Ateshian GA, Sundstrom LE: A tissue level tolerance criterion for living brain developed with an in vitro model of traumatic mechanical loading. Stapp Car Crash Journal 47: 93-105, 2003.
27.
Park S, Hung CT, Ateshian GA: Mechanical response of bovine articular cartilage under dynamic unconfined compression loading at physiologic stress levels. Osteoarthritis Cartilage , 12(1):65-73,
2004.
28.
Ateshian GA, Chahine NO, Basalo IM, Hung CT: The correspondence between equilibrium biphasic and triphasic material properties in mixture models of articular cartilage. J Biomechanics 37, 391-
400, 2004.
29.
Hung CT, Mauck RL, Wang CC-B, Lima EG, Ateshian GA: A paradigm for functional tissue engineering of articular cartilage via applied physiologic deformational loading. Ann Biomed Eng
32(1), 35-49, 2004.
30.
Finkelstein E, Chang W, Chao PHG, Gruber D, Hung CT, Bulinski C: Roles of microtubules, cell polarity, and adhesion in electric field-mediated motility. J Cell Science 117(8),1533-1545, 2004.
Clark T. Hung, Ph.D. 17.

31.
Chahine NO, Wang CC-B, Hung CT, Ateshian GA: Anisotropic strain-dependent material properties of bovine articular cartilage in the transitional range from tension to compression. J Biomechanics 37,
1251-1261, 2004.
32.
Kelly TN, Wang CC-B, Mauck RL, Ateshian GA, Hung CT: Role of cell-associated matrix on material development of free-swelling and dynamically loaded chondrocyte-seeded agarose gels.
Biorheology , 41, 223-237, 2004.
33.
Lima EG, Mauck RL, Han S, Park S, Ng KW, Ateshian GA, Hung CT: Functional tissue engineering of chondral and osteochondral constructs. Biorheology 41, 577-590, 2004.
34.
Takai E, Mauck RL, Hung CT, Guo XE: Osteocyte viability and regulation of osteoblast function in a
3D trabecular bone explant under dynamic hydrostatic pressure. J Bone Min Res 19(9):1403-1410,
2004.
35.
Basalo IM, Mauck RL, Kelly TN, Nicoll SB, Chen FH, Hung CT, Ateshian GA: Cartilage interstitial fluid load support in unconfined compression following enzymatic digestion. J Biomechanics 126,
779-786, 2004
36.
Krishnan R, Caligaris M, Mauck RL, Hung CT, Costa KD, Ateshian GA: Removal of the superficial zone of articular cartilage does not increase its frictional coefficient. Osteoarthritis Cartilage 12(12),
947-955, 2004.
37.
Lu HH, Jiang J, Tang A, Hung CT, Guo XE: Development of controlled heterogeneity on a polymerceramic hydrogel scaffold for osteochondral repair. Bioceramics , 17:609-613, 2004.
38.
Ng KW, Wang CC-B, Mauck RL, Kelly TN, Chahine NO, Costa KD, Ateshian GA, Hung CT: A layered agarose approach to fabricate depth-dependent inhomogeneity in chondrocyte-seeded constructs. J Orthop Res 23(1), 134-141,2005.
39.
Chao PHG, Tang Z, Angelini E, West AC, Costa KD, Hung CT: Dynamic osmotic loading of cells using a novel microfluidic device. J Biomechanics 38(6), 1273-1281, 2005.
40.
Basalo IM, Raj D, Krishnan R, Chen FH, Hung CT, Ateshian GA: Effects of enzymatic degradation on the frictional response of articular cartilage in stress relaxation. J Biomechanics 38(6), 1343-1349,
2005.
41.
Takai E, Costa KD, Shaheen A, Hung CT, Guo XE: Osteoblast elastic modulus measured by atomic force microscopy is substrate dependent, Ann Biomed Eng , 33(7), 963-971, 2005.
42.
Gupta R, Truong L, Bear D, Chafik D, Modafferi E, Hung CT: Shear stress alters the expression of
Myelin Associated Glycoprotein (MAG) and Myelin Basic Protein (MBP) in Schwann cells. J Orthop
Res 23(5), 1232-9, 2005.
43.
Chahine NO, FH Chen, Hung CT, Ateshian GA: Direct Measurement of Osmotic Pressure of glycosaminoglycan solutions by membrane osmometry at room temperature. Biophysical Journal
89(3),1543-50, 2005.
44.
Ateshian GA and Hung CT: Patellofemoral joint biomechanics and tissue engineering. Clin Orthop
Res Relat Res 436:81-90, 2005.
45.
Kelly TN, Ng KW, Wang CCB, Ateshian GA, Hung CT: Spatial and temporal development of chondrocyte-seeded agarose constructs in free-swelling and dynamically loaded cultures. J
Biomechanics 39(8):1489-1497, 2006.
46.
Ateshian GA, Likhitpanichkul M, Hung CT: A mixture theory analysis of passive transport in osmotic loading of cells. J Biomechanics 39(3):464-75, 2006
47.
Basalo IM, Chen FH, Hung CT, Ateshian GA: Frictional response of bovine articular cartilage under creep loading following proteoglycan digestion with chondroitinase ABC . J Biomech Eng 128(1):131-
134, 2006.
Clark T. Hung, Ph.D. 18.

48.
Takai E, Landesberg R, Katz RW, Hung CT, Guo XE: Substrate modulation of osteoblast adhesion strength, focal adhesion kinase activation, and responsiveness to mechanical stimuli. Mol Cell
Biomech.
3(1):1-12, 2006.
49.
Ishii Y, Thomas AO, Guo XE, Hung CT, Chen FH: Localization and distribution of Cartilage
Oligomeric Matrix Protein in the rat intervertebral disc. Spine 31(14):1539-1546, 2006.
50.
Ng KW, Mauck RL, Statman LY, Lin EY, Ateshian GA, Hung CT: Dynamic deformational loading results in selective application of mechanical stimulation in a layered, tissue-engineered construct.
Biorheology 43(3-4): 497-507, 2006.
51.
Ho MH, Kelly TN, Guo XE, Ateshian GA, Hung CT: Spatially varying material properties of the rat intervertebral disc. Spine 31(15):E486-93, 2006
52.
Chao PHG, West AC, Hung CT: Chondrocyte intracellular calcium, cytoskeletal organization and gene expression responses to dynamic osmotic loading. Am J Physiol (Cell Physiology) 291(4):C718-
25, 2006
53.
Ateshian GA and Hung CT: The natural synovial joint: properties of cartilage. J Eng Tribology:
220(J8):657-670.
54.
Ateshian GA, Costa KD, Hung CT: A theoretical analysis of water transport through chondrocytes.
Biomech Model Mechanobiol 6(1-2):91-101, 2007.
55.
Chahine NO, Ateshian GA, Hung CT: The effect of finite compressive strain on chondrocyte viability in statically loaded bovine articular cartilage.
Biomech Model Mechanobiol 6(1-2):103-111, 2007.
56.
Basalo IM, Chahine NO, Kaplun M, Chen FH, Hung CT, Ateshian GA: Chondroitin sulfate reduces the friction coefficient of articular cartilage. J Biomechanics, 40(8):1847-1854, 2007.
57.
Guo XE, Takai E, Jiang XJ, Xu Q, Whitesides GM, Yardley JT, Hung CT, Chow E, Hantschel T,
Costa KD: Intracellular calcium waves in bone cell networks under single cell nanoindentation. Mol
Cell Biomech 3(3):95-107, 2007.
58.
Lima EG, Bian L, Ng KW, Mauck RL, Byers BA, Tuan RS, Ateshian GA, Hung CT: The effect of applied compressive loading on tissue-engineered cartilage constructs cultured with TGF 3.
Osteoarthritis Cartilage , 15(9): 1025-1033, 2007.
59.
Chahine NO, Hung CT, Ateshian GA: In situ measurements of chondrocyte deformation under transient loading. Eur Cell Mater , 13:100-111, 2007.
60.
Chao PG, Lu HH, Hung CT*, Nicoll SB, Bulinski JC: Effects of applied DC electric field on ligament fibroblast migration and wound healing. Connect Tissue Res , 48(4):188-197, 2007. (*corresponding author)
61.
Albro MB, Chahine NO, Caligaris M, Wei VI, Likhitpanichkul M, Ng KW, Hung CT, Ateshian GA:
Osmotic loading of spherical gels: a biomimetic study of hindered transport in the cell protoplasm. J
Biomech Eng, 129(4):503-510, 2007.
62.
Finkelstein EI, Chao PH, Hung CT, Bulinski JC: Electric field-induced polarization of charged cell surface proteins does not determine the direction of galvanotaxis. Cell Motil Cytoskeleton
64(11):833-846, 2007.
63.
Ng KW, Saliman JD, Lin EY, Statman LY, Kugler LE, Lo SB, Ateshian GA, Hung CT: Culture duration modulates collagen hydrolysate-induced tissue remodeling in chondrocyte-seeded agarose hydrogels. Ann Biomed Eng 35(11):1914-1923, 2007.
64.
Ng KW, DeFrancis JG, Kugler LE, Kelly TN, Ho MM, O’Conor CJ, Ateshian GA, Hung CT: Amino acids supply in culture media is not a limiting factor in the matrix synthesis of engineered cartilage tissue. Amino Acids 35(2):433-438, 2008.
65.
Kelly TN, Fisher MB, Oswald ES, Tai T, Mauck RL, Ateshian GA, Hung CT: Low-serum media and dynamic deformational loading in tissue engineering of articular cartilage, Ann Biomed Eng
36(5):769-779, 2008.
Clark T. Hung, Ph.D. 19.

66.
Bian LM, Lima EG, Angione SL, Ng KW, Williams DY, Xu D, Stoker AM, Cook JL, Ateshian GA,
Hung CT: Long-term maintenance of chondral and osteochondral explants in serum-free culture. J
Biomechanics 41(6):1153-1159, 2008.
67.
Lima EG, Tan AR, Tai T, Bian L, Stoker AM, Ateshian GA, Cook JL, Hung CT: Differences in interleukin-1 response between engineered and native cartilage.
Tissue Eng 14(10):1721-1730, 2008.
68.
Huo B, Lu XL, Hung CT, Costa KD, Xu Q, Whitesides GM, Guo XE: Fluid flow induced calcium response in bone cell network. Cell Mol Bioeng , 1(1):58-66, 2008.
69.
Moffat KL, Sun W-H S, Pena PE, Chahine NO, Doty SB, Ateshian GA, Hung CT, Lu HH:
Characterization of the compressive mechanical properties and mineral distribution at the ligament-tobone insertion site. Proc Natl Acad Sciences , 105(23):7947-7952 , 2008.
70.
Kelly TN, Ng KW, Ateshian GA, Hung CT: Analysis of radial variations in material properties and matrix composition of chondrocyte-seeded agarose hydrogel constructs. Osteoarthritis Cartilage
17(1):73-82, 2009. (PMID: 18805027)
71.
Ng KW, Kugler LE, Doty SB, Ateshian GA, Hung CT: Scaffold degradation elevates the collagen content and dynamic compressive modulus in engineered articular cartilage, Osteoarthritis Cartilage
17(2):220-227, 2009. (PMID: 18801665)
72.
Lima EG, Chao PG, Ateshian GA, Bal S, Cook JL, Vunjak-Novakovic G, Hung CT : The effect of devitalized trabecular bone on the formation of osteochondral tissue-engineered constructs,
Biomaterials 29(32):4292-4299, 2008.
73.
Lima EG, Tan AR, Tai T, Marra KG, DeFail A, Ateshian GA, Hung CT: Genipin enhances the mechanical properties of tissue engineered cartilage and protects against inflammatory degradation when used as a medium supplement, J Biomed Mater Res A 91(3):692-700. (PMID: 19025982)
74.
Lima EG, Tan AR, Tai T, Bian L, Ateshian GA, Cook JL, Hung CT: Physiologic deformational loading does not counteract the catabolic effects of interleukin-1 in long-term culture of chondrocyteseeded agarose constructs, J Biomechanics 41(15):3253-3259, 2008. (PMID: 18823628)
75.
Albro MB, Chahine NO, Li R, Yeager K, Hung CT, Ateshian GA: Dynamic loading of deformable porous media can induce active solute transport. J Biomechanics , 41(15):3152-3157, 2008. (PMID:
18922531)
76.
Canal CE, Hung CT, Ateshian GA: Two-Dimensional Strain Fields on the Cross-Section of the
Bovine Humeral Head Under Contact Loading. J Biomechanics 41(15):3145-3151, 2008. (PMID:
18952212)
77.
Bian L, Angione SL, Ng KW, Williams DY, Mao DQ, Ateshian GA, Hung CT: Influence of decreasing nutrient path length on the development of engineered cartilage, Osteoarthritis Cartilage ,
17(5):677-685, 2009
78.
Oswald ES, Chao PG, Bulinski JC, Ateshian GA, Hung CT: Zonal variation in chondrocyte water transport properties and proteoglycan production in juvenile bovine cartilage do not depend on osmotic environment. Cell Mol Bioeng 1(4): 339-348, 2008.
79.
Bian L, Kaplun M, Williams DY, Xu D, Ateshian GA, Hung CT: Influence of chondroitin sulfate on the biochemical and mechanical properties of cartilage explants in long term culture. J Biomechanics
42(3):286-90, 2009.
80.
Chahine NO, Albro BM, Lima EG, Wei VI, Dubois CR, Hung CT, Ateshian GA: Effect of dynamic loading on the transport of solutes into agarose hydrogels. Biophys J 97(4):968-975, 2009.
81.
Bian L, Crivello K, Ng KW, Xu D, Williams DY, Ateshian GA, Hung CT: Influence of temporary chondroitinase ABC-induced GAG suppression on maturation of tissue engineered cartilage. Tissue
Engineering 15(8):2065-2072, 2009.
Clark T. Hung, Ph.D. 20.

82.
Ng KW, Ateshian GA, Hung CT: Zonal chondrocytes seeded in a layered agarose hydrogel create engineered cartilage with depth-dependent cellular and mechanical inhomogeneity, Tissue
Engineering, 15(9):2315-2324, 2009.
83.
Hung CT: One breath closer to making engineered tissues a clinical reality. Cell Stem Cell , 4(1):5-6,
2009.
84.
Canal CE, Gardner T, Rajan V, Ahmad C, Hung CT, Ateshian GA: Two-Dimensional Strain Fields on the Cross-Section of the Human Patellofemoral Joint under Physiological Loading, Biomechanics ,
41(15):3145-3151, 2009.
85.
Ateshian GA, Costa KD, Azeloglu EU, Morrison III B, Hung CT: Continuum modeling of biological tissue growth by cell division, and alteration of intracellular osmolytes and extracellular fixed charge density. J Biomech Eng , 131(10):101001, 2009
86.
Ateshian GA, Rajan V, Chahine NO, Canal CE, Hung CT: Modeling the matrix of articular cartilage using a continuous fiber angular distribution predicts many observed phenomena. J Biomech
131(6):061003, 2009.
87.
Ng KW, Mauck RL, Wang CCB, Kelly TN, Ho MMY, Chen FH, Ateshian GA, Hung CT: Duty cycle of deformational loading influences the growth of engineered articular cartilage. Cell Mol Bioeng
2(3): 386-394, 2009.
88.
Albro M, Rajan V, Li R, Hung CT, Ateshian GA: Characterization of the concentration-dependence of solute diffusivity and portioning in a model dextran-agarose transport system. Cell Mol Bioeng
2(3):295-305, 2009.
89.
Albro M, Petersen LE, Li R, Hung CT, Ateshian GA: Influence of partitioning of osmolytes by the cytoplasm on the passive response of cells to osmotic loading. Biophys J , 97(11):2886-93, 2009.
90.
Bian L, Stoker AM, Marberry KM, Ateshian GA, Cook JL, Hung CT: Effects of Dexamethasone on the Functional Properties of Cartilage Explants during Long-term Culture. Am J Sports Med 38(1):78-
85, 2010.
91.
Ng KW, Lima EG, Bian L, O’Conor CJ, Jayabalan PS, Stoker AM, Kuroki K, Cook CR, Ateshian
GA, Cook JL, Hung CT: Passaged adult chondrocytes can form engineered cartilage with functional mechanical properties: A canine model. Tissue Engineering A 16(3):1041-1051, 2010.
92.
Bian L, Fong JV, Lima EG, Stoker AM, Ateshian GA, Cook JL, Hung CT: Dynamic mechanical loading enhances functional properties of tissue engineered cartilage using mature canine chondrocytes. Tissue Engineering Part A 16(5):1781-1790, 2010.
93.
Grayson WL, Bhumiratana S, Chao GPH, Hung CT, Vunjak-Novakovic G: Spatial regulation of human mesenchymal stem cell differentiation in engineered osteochondral constructs: effects of predifferentiation, soluble factors and medium perfusion. Osteoarthritis Cartilage 18(5):714-723, 2010
94.
Guterl CC, Hung CT, Ateshian GA: Electrostatic and non-electrostatic contributions of proteoglycans to the compressive equilibrium modulus of bovine articular cartilage. J Biomech 43(7):1343-1350,
2010.
95.
Ateshian GA, Morrison B 3 rd , Hung CT: Modeling of active transmembrane transport in a mixture theory framework. Ann Biomed Eng 38(5):1801-1814, 2010.
96.
Jiang J, Tang A, Ateshian GA, Guo XE, Hung CT, Lu HH: Bioactive stratified polymer ceramichydrogel scaffold for integrative osteochondral repair. Ann Biomed Eng 38(6):2183-2196, 2010.
97.
Albro MB, Li R, Banerjee RE, Hung CT, Ateshian GA: Validation of theoretical framework explaining active solute uptake in dynamically loaded porous media. J Biomech 2010 Aug
26;43(12):2267-7, 2010.
98.
Hung CT: Transient receptor potential vanilloid 4 channel as an important modulator of chondrocyte mechanotransduction of osmotic loading. Arthritis Rheum 62(10):2850-1, 2010.
Clark T. Hung, Ph.D. 21.

99.
Tan AR, Dong EY, Ateshian GA, Hung CT: Response of engineered cartilage to mechanical insult depends on construct maturity. Osteoarthritis Cartilage, 18(12):1577-1585, 2010.
100.
Tan AR, Dong EY, Andry JP, Bulinsk JC, Ateshian GA, Hung CT: Co-culture of engineered cartilage with freshly harvested primary chondrocytes induces expedited growth. Clin Orthop ,
469(10):2735-2743, 2011.
101.
Hung CT, Cook JL: Clinically relevant strategies for treating cartilage and meniscal pathology: editorial comment. Clin Orthop Rel Res , 469(10):2677-2678, 2011.
102.
Jayabalan P, Tan AR, Rahaman MN, Bal S, Hung CT, Cook JL: Bioactive glass 13-93 as a subchondral substrate for tissue-engineered osteochondral constructs- A Pilot Study. Clin Orthop , in press, 2011.
103.
Ateshian GA, Weiss JA, Hung CT: The role of mass balance equations in growth mechanics illustrated in surface and volume dissolution. J Biomech Eng , in press, 2011.
104.
Albro MB, Banerjee RE, Li R, Oungoulian SR, Chen B, Del Palomar AP, Hung CT, Ateshian GA:
Dynamic loading of immature epiphyseal cartilage pumps nutrients out of vascular canals. J Biomech,
44(9):1654-165 9 , 2011.
105.
Sampat SR, O’Connell GD, Fong JV, Aguaron EA, Ateshian GA, Hung CT. Synovium derived stem cells for cartilage tissue engineering. Tissue Eng Part A , 17(17-18):2259-2265, 2011.
106.
Gunja NJ, Chen A, Dujari D, Luengo A, Fong JV, Hung CT: Migration response of outer and inner meniscus cells to applied direct current electric fields. J Orthop Res , in press, 2011.
107.
Oswald ES, Brown LM, Bulinski JC, Hung CT: Label-free protein profiling of adipose-derived human stem cells under hyperosmotic treatment. J Proteome Res , 10(7):3050-3059, 2011.
108.
Ng KW, O’Conor CJ, Kugler LE, Cook JL, Ateshian GA, Hung CT: Transient supplementation of anabolic growth factors rapidly stimulates matrix synthesis in engineered cartilage. Ann Biomed Eng .
39(10):2491-2500, 2011.
109.
Oswald ES, Ahmed HS, Kramer SP, Bulinski JC, Ateshian GA, Hung CT: Effects of hypertonic
(NaCl) two-dimensional and three-dimensional culture conditions on the properties of cartilage tissue engineered from an expanded mature bovine chondrocyte source. Tissue Eng C , in press, 2011
BOOK CHAPTERS
1.
Mow VC and Hung CT: Chapter 3. Biomechanics of Articular Cartilage in Basic Biomechanics of the
Musculoskeletal System , 3 rd edition, ed. N Nordin and V Frankel, Williams & Wilkins, Baltimore,
MD, p60-100, 2001.
2.
Tang Z, Chao G, Tucay A, Takai E, Djukic D, Lind ML, Hung CT, Guo XE, West A, Osgood R,
Yardley JT: XYZ on a Chip: Nanoscale Fabrication, Fluidics, and Optics Directed Toward
Applications within Biology and Medicine. In Organic Nanophotonics (NATO Science Series. Series
II, Mathematics, Physics, and Chemistry, V. 100), eds. F Charra, VM Agranovich, F Kajzar, Kluwer
Academic Publishers, p127-138, 2003.
3.
Ateshian GA and Hung CT: Chapter 4. Functional Properties for Articular Cartilage and Tissue
Engineered Constructs in Functional Tissue Engineering , eds. F Guilak, DL Butler, SA Goldstein, DJ
Mooney, Springer-Verlag, New York, NY, p46-68, 2003.
4.
Mow VC and Hung CT: Chapter 7.2.1.4 Mechanical Properties of Normal and Osteoarthritic Articular
Cartilage, and the Mechanobiology of Chondrocytes in Osteoarthritis , 3 rd edition, ed. KD Brandt, S
Lohmander and M Doherty, Oxford, Oxford University Press, p102-112, 2003.
Clark T. Hung, Ph.D. 22.

5.
Guilak F and Hung CT: Physical Regulation of Cartilage Metabolism in Basic Orthopaedic
Biomechanics and Mechanobiology, 3 rd edition, eds. VC Mow and R Huiskes, Lippincott Williams &
Wilkins, Baltimore, MD, 2005.
6.
Hung CT and Mauck RL: Chapter 15: Biological Assays: Cellular Level in Biomedical Technology and Devices Handbook , eds. J Moore and G. Zouridakis, CRC Press, Boca Raton, p15.1-15.39, 2003.
7.
Kelly TN, Wang CC-B, Mauck RL, Ateshian GA, Hung CT: Role of Cell-associated Matrix on
Material Development of Free-Swelling and Dynamically Loaded Chondrocyte-seeded Agarose Gels in Mechanobiology: Cartilage and Chondrocyte , ed. J.-F. Stoltz, IOS Press, Amsterdam, The
Netherlands, Vol. 3, p223-237, 2004.
8.
Lima EG, Mauck RL, Han S, Park S, Ng KW, Ateshian GA, Hung CT: Functional Tissue Engineering of Chondral and Osteochondral Constructs in Mechanobiology: Cartilage and Chondrocyte , ed. J.-F.
Stoltz, IOS Press, Amsterdam, The Netherlands, Vol. 3: 577-590, 2004.
9.
Ng KW, Mauck RL, Statman LY, Lin EY, Ateshian GA, Hung CT. Dynamic deformational loading results in selective application of mechanical stimulation in a layered, tissue-engineered cartilage construct. Mechanobiology: Cartilage and Chondrocyte, ed. J.-F. Stoltz, IOS Press, Amsterdam,
Netherlands, Vol. 4: 497-507, 2006.
10.
Huang AH, Hung CT, Mauck RL: Functional cartilage tissue engineering with adult stem cells: current status and future directions in Stem Cells for Regenerative Medicine (E-Book), edited by
Herman Cheung, Bentham Science Publishers, 2010.
11.
Gunja N, Hung CT, Bulinski JC: Effects of DC Electric Fields on Cells of the Musculoskeletal System
(Chapter 9) in The Physiology of Bioelectricity in Development, Tissue Regeneration, and Cancer by
CRC Press (edited by Christine Pullar), 2011.
12.
Tan AR and Hung CT: Engineering Functional Cartilage Grafts in Stem Cell Biology and
Regenerative Medicine (Chapter 13) by Springer Science (edited by HS Bernstein), 2011 (in press)
CONFERENCE ABSTRACTS
1.
Williams JL, Hung CT: A new biaxial testing method for producing uniform and isotropic strains in membranes used as cell or tissue substrates. Adv Bioeng ASME BED-26:411-413, 1993.
2.
Hung CT, Pollack SR, Reilly TM, Brighton CT: Real-time calcium response of bone cells exposed to fluid flow. Oral presentation and abstract at the 13th Annual Meeting of the Bioelectrical Repair and
Growth Society, Dana Point, CA, October 11-14, 1993.
3.
Gelb ID, Reilly TM, Brighton CT, Pollack SR, Hung CT: Platelet-derived growth factor stimulation of bone cells: intracellular calcium and inositol trisphosphate as second messengers. Trans Orthop Res
Soc 19:349, 1994.
4.
Reilly TM, Hung CT, Gelb ID, Brighton CT: The effects of calcium second messenger inhibition in the stress-induced proliferation of bone cells. Trans Orthop Res Soc 19:274, 1994.
5.
Hung CT, Reilly TM, Pollack SR, Brighton CT: Heterogeneous [Ca 2+ ] i exposed to fluid flow.
Trans Orthop Res Soc 19:498, 1994.
response in bone cells
6.
Hung CT, Reilly TM, Pollack SR, Brighton CT: Real time [Ca 2+ ] i measurements in bone cells exposed to fluid flow. BME’94 International Conference on Biomedical Engineering, Hong Kong,
April 7-9, 1994.
7.
Hung CT, Pollack SR: Heterogeneous [Ca
The Netherlands, July 10-15, 1994.
2+ ] i response as a mediator of Wolff's Law in bone cells exposed to laminar fluid flow. Proceeds of 2nd World Congress of Biomechanics II:241b, Amsterdam,
Clark T. Hung, Ph.D. 23.

8.
Allen FD, Pollack SR, Gupta R, Hung CT: The intracellular calcium response of osteoblast-like cells to 60 kHz electric field stimulation from 10 V/m to 1 kV/m. Trans SPRBM 14:3, 1994.
9.
Rau V, Hung CT, Pollack SR: Measuring the zeta potential of primary bone cells. Trans SPRBM
14:28, 1994.
10.
Hung CT, Allen FD, Pollack SR, Brighton CT: Elucidating the cell signaling pathway in the calcium response of bone cells to fluid flow. ASME BED 25:569-570, 1995.
11.
Allen FD, Hung CT, Pollack SR, Brighton CT: The effect of newborn bovine serum on the intracellular calcium concentration response of primary cultured bone cells to fluid flow. Proceeds of
2nd Intl Conference on Cellular Engineering , p3, La Jolla, CA, August 19-22, 1995.
12.
Hung CT, Allen FD, Pollack SR, Brighton CT: The role of the convective current density in the calium response of cultured bone cells to fluid flow.
Proceeds of the 2nd Inl Conference on Cell Eng , p46, La Jolla,CA, Aug 19-22, 1995.
13.
Allen FD, Hung CT, Pollack SR, Brighton CT: Comparison of the [Ca 2+ ] i response of cultured primary, MC3T3-E1, and ROS 17/2.8 osteoblast-like cells to fluid flow. Trans SPRBM 15:52, 1995.
14.
Hung CT, Allen FD, Mansfield KD, Shapiro IM: Extracellular ATP modulates [Ca 2+ ] i treated emryonic chondrocytes. Trans Orthop Res Soc 22(1):7, 1997. in retinoic acid
15.
Hung CT, Allen FD, Pollack SR, Attia ET, Hannafin JA, Torzilli PA: Comparison of the fluidinduced calcium response of ACL and MCL fibroblasts. Trans Orthop Res Soc 22(1):54, 1997.
16.
Allen FD, Hung CT, Pollack SR, Brighton CT: Pertussis toxin inhibits cytosolic calcium increases in osteoblast-like cells stimulated by laminar fluid flow incorporating serum. Trans Orthop Res Soc
22(1):706, 1997.
17.
Allen FD, Hung CT, Pollack SR, Brighton CT: The effect of cell culture passaging on the transduction of fluid flow shear stress in osteoblast-like cells. Trans Orthop Res Soc 22(1):707, 1997.
18.
Hung CT, Valhmu WB, Ratcliffe A, Mow VC, Allen FD: Articular chondrocyte calcium response to transient fluid-induced shear stress. Adv Bioeng BED 35:115-116, 1997.
19.
Gu WY, Lai WM, Hung CT, Liu ZP, Mow VC: Analysis of transient swelling and electrical responses of an isolated cell to sudden osmotic loading. Adv Bioeng BED-36, 1997.
20.
Mow VC, Gu WY, Hung CT, Liu ZP, Lai WM: Transient swelling and electrical responses of cells subject to osmotic shock loading. Trans 3rd Intl Cell Eng Conference , San Remo, Italy, 1997.
21.
Hung CT, Johnson ED, Allen FD, Litt M, Pollack SR, Meaney DF: Changes in osteoblasts under time averaged microgravity conditions. Advances in Heat and Mass Transfer in Biotechnology HTD-
355/BED-37, 1997.
22.
Hung CT, Valhmu WB, Mow VC, Ratcliffe A: Calcium signaling in articular chondrocytes subjected to transient fluid-induced shear. Trans Orthop Res 23(2):897, 1998.
23.
Valhmu WB, Hung CT, Mow VC, Ratcliffe A: Fluid flow stimulates the ERK1/2, but not
SAPK/JNK, cascade in bovine articular chondrocytes, Trans Orthop Res 23(1):184, 1998.
24.
Hung CT, Gu WY, Lai WM, Mow VC: Quantification of transient swelling behavior in cultured chondrocytes to osmotic loading, Trans Orthop Res 23(2):870, 1998.
25.
Hung CT, Attia ET, Bhargava M, Hannafin JA, Torzilli PA: Mechano-chemical coupling in ACL and
MCL cells: Calcium response to transient fluid-induced shear stress and bradykinin, Trans Orthop Res
23(2): 578, 1998.
26.
Hung CT, Wang CB, Henshaw DR, Goldring MB, Valhmu WB: Toward defining the role of Ca 2+ and
MAP Kinases in fluid-induced shear regulation of aggrecan in chondrocytes. Advances in
Bioengeering BED-39, p59-60, 1998.
Clark T. Hung, Ph.D. 24.

27.
Hung CT, Gu WY, Lai WM, Mow VC: A new method to determine mechano-electrochemical properties of cells. Proceeds of the Third World Congress of Biomechanics , p215b, Sapporo, Japan,
August 2-8, 1998.
28.
Hung CT, Gu WY, Lai WM, Mow VC: Chondrocyte swelling experiments and theoretical predictions. Proceeds of the Third World Congress of Biomechanics , p111b, Sapporo, Japan, August
2-8, 1998.
29.
Palmer G, Ho J, Hung CT, Ratcliffe A, Valhmu WB: MEK-1 regulates aggrecan gene expression through exon 1 (5’UTR). Trans Orthop Res Soc 24(1), p81, 1999.
30.
Soltz MA, Mauck RL, Hung CT, Ateshian GA: Fluid pressurization in agarose hydrogels under cyclic loading. Advances in Bioengeerin g BED-42, p127-128, 1999.
31.
Mow VC, Sun DN, Guo XE, Hung CT, Lai WM: Chondrocyte-extracellular matrix interactions during osmotic swelling. Adv Bioeng BED-42, p133-134, 1999.
32.
Mauck RL, Jezyk M, Valhmu WB, Hung CT: Chondrocyte shape change and migratory response to
DC Electric Fields. Adv Bioeng BED-42, p341-342, 1999.
33.
Soltz MA, Stankiewicz A, Mauck RL, Hung CT, Ateshian GA: Direct hydraulic permeability measurements of agarose hydrogels used as cell scaffolds. Advances in Bioengeering BED-43, 229-
230, 1999.
34.
Mauck RL, Chao P-H, Gilbert B, Valhmu WB, Hung CT: Chondrocyte translocation and orientation to applied DC electric fields. Advances in Bioengeering BED-43, 117-118, 1999.
35.
Mauck RL, Soltz MA, Raia FA, Ateshian GA, Valhmu WB, Hung CT: Time-varying effect of ascorbate on the compressive modulus and GAG content of chondrocyte-seeded agarose cultures. Adv
Bioeng BED-43, 105-106, 1999.
36.
Soltz MA, Mauck RL, Hung CT, Ateshian GA: Is articular cartilage orthotropic in compression? Adv
Bioeng BED-43, 209-210, 1999.
37.
Palmer G, Chao PG, Raia F, Mauck R, Valhmu W, Hung CT: Osmotic loading regulates chondrocyte cell size and aggrecan gene expression. Trans Orthop Res Soc 25, 924, 2000.
38.
Wang, CC-B, Soltz MA, Mauck RL, Valhmu WB, Ateshian GA, Hung CT: Comparison of the equilibrium strain distribution in articular cartilage explants and cell-seeded alginate disks under compression. Trans Orthop Res Soc 25, 131, 2000.
39.
Soltz MA, Palma C, Barsoumian S, Wang CC-B, Hung CT, Ateshian GA: Multi-axial loading of bovine articular cartilage in unconfined compression. Trans Orthop Res Soc 25, 888, 2000.
40.
Valhmu W, Palmer G, Wang C, Raia F, Mauck R, Chao P, Hung CT: Fluid flow modulates aggrecan gene expression through the mitogen-activated protein kinase pathway. Trans Orthop Res Soc 25, 82,
2000.
41.
Mauck RL, Soltz MA, Seruya M, Valhmu WB, Ateshian GA, Hung CT: Characterization of alginate for tissue engineering. Trans Orthop Res Soc 25, 200, 2000.
42.
Allen FD, Hung CT, Pollack SR, Brighton CT: Fluid flow mechanotransduction to intracellular calcium signalling in primary bone cells depends on fluid-induced shear stress magnitude and loading rate. Trans Orthop Res Soc 25, 632, 2000.
43.
Allen FD, Hung CT, Pollack SR, Brighton CT: Mechano-chemical coupling in the flow-induced activation of intracellular calcium signaling in primary cultured bone cells. Trans Orthop Res Soc 25,
634, 2000.
44.
Mauck RL, Palmer GD, Wang CC-B, Soltz MA, Valhmu WB, Ateshian GA, Hung CT. Dynamic compression stimulates the development of equilibrium aggregate modulus in tissue engineered cartilage constructs. Adv Bioeng BED-48, p67-68, 2000.
Clark T. Hung, Ph.D. 25.

45.
Chao P-H, Roy R, Mauck RL, Liu W, Hung CT. Electric field-induced chondrocyte migration:
Neomycin effects. Adv Bioeng BED-48, p65-66. 2000.
46.
Wang CC-B, Guo XE, Deng JJ, Mow VC, Ateshian GA, Hung CT: A novel non-invasive technique for determining distribution of fixed charge density within articular cartilage. Trans Orthop Res Soc
26, 129, 2001.
47.
Chao P-HG, Palmer G, Valhmu WB, Thomas JL, Hung CT: Characterization of the chondrocyte response to hyperosmotic loading- influence of medium constituents. Trans Orthop Res Soc 26, 557,
2001.
48.
Hung CT, Jamieson KV, Roy R, Wong DD, Chao P-HG, Sun DDN, Guo XE: Comparison of transient chondrocyte swelling and shrinking behavior. Trans Orthop Res Soc 26, 559, 2001.
49.
Mauck RL, Seyhan SL, Wang CC-B, Soltz MA, Chao P-HG, Valhmu WB, Ateshian GA, Hung CT:
Physiologic loading for cartilage tissue engineering- effect of chondrocyte seeding density. Trans
Orthop Res Soc 26, 622, 2001.
50.
Mauck RL, Wang CC-B, Seyhan SL, Kelly TN, Valhmu WB, Ateshian GA, Hung CT: Seeding density influences the development of mechanical properties in agarose constructs. Trans Orthop Res
Soc 26, 432, 2001.
51.
Soltz, MA, Mauck RL, Hung CT, Lai W, Ateshian GA: Osmotic pressure influence on the frictional response of articular cartilage. Trans Orthop Res Soc 26, 60, 2001.
52.
Chao P-HG, Palmer GD, Mauck RL, Guo XE, Hung CT: Aggrecan gene expression of chondrocyte seeded 3D hydrogel cultures in response to hypertonic loading. Adv Bioeng BED-50, 205-206, 2001.
53.
Hung CT and Nicoll SB: Tissue and cell culture techniques: an overview. Adv Bioeng BED-50, 317-
318, 2001.
54.
Hung CT, Costa KD, Guo XE: Apparent and transient mechanical properties of chondrocytes during osmotic loading using triphasic theory and AFM indentation. Adv Bioeng BED-50, 625-626, 2001.
55.
Takai E, Landesberg R, Katz RW, Hung CT, Guo XE: Osteoblast cell adhesion and focal adhesion kinase activation on various substrates. Adv Bioeng BED-50, 669-670, 2001.
56.
Mauck RL, Seyhan, SL, Nicoll SB, Ateshian GA, Hung CT: Transforming growth factor  1 increases the mechanical properties and matrix development of chondrocyte-seeded agarose hydrogels. Adv
Bioeng BED-50, 691-692, 2001.
57.
Wang CC-B, Chahine NO, Kelly TN, Valhmu WB, Hung CT, Ateshian GA: Optical determination of anisotropic material properties of bovine articular cartilage in compression. Adv Bioeng BED-50, 719-
720, 2001.
58.
Hung CT, Chao P-HG, Mauck RL, Guo XE, Mow VC: Chondrocyte aggrecan gene expression in response to hypertonic osmotic loading: comparison of monolayer and 3D studies. Submitted to the
Euromech420 Colloquium on Mechanobiology of Cells and Tissues in Nancy, France, April 24-26,
2001.
59.
Wang CC-B, Guo XE, Ateshian GA, Mow VC, Hung CT: Determination of the chondrocyte environment within cartilage subjected to loading: theoretical and experimental approach. Submitted to the 2 nd International Symposium on the Mechanobiology of Chondrocytes and Cartilage in Paris,
France, April 27-28, 2001. (satellite meeting of the Euromech420 Colloquium)
60.
Mauck RL, Soltz MA, Ateshian GA, Hung CT: Dynamic hydrostatic pressurization increases matrix gene expression by chondrocytes in 3D culture. Adv Bioeng IMECE2001/BED-23147.
61.
Wang CC-B, Chahine NO, Kelly TN, Lai WM, Hung CT, Ateshian GA: The strain-softening of bovine articular cartilage under infinitesimal deformation in unconfined compression. Adv Bioeng
IMECE2001/BED-23061.
Clark T. Hung, Ph.D. 26.

62.
Kelly TN, Wang CC-B, Chahine NO, Ateshian GA, Hung CT: Temporal development of material properties in free swelling chondrocyte-seeded agarose constructs.
Adv Bioeng IMECE2001/BED-
23144.
63.
Andarawis NA, Seyhan SL, Mauck RL, Soltz MA, Ateshian GA, Hung CT: A novel device for direct permeation measurements of hydrogels and soft hydrated tissues. Adv Bioeng IMECE2001/BED-
23149.
64.
Lo SS, Mauck RL, Seyhan SL, Palmer GD, Mow VC, Hung CT: Mechanical loading modulates gene expression in chondrocyte-seeded agarose hydrogels. Adv Bioeng IMECE2001/BED-23145.
65.
Lo SS, LeRoux MA, Chao PG, Palmer GD, Hung CT: The effect of osmolarity on gene expression of chondrocyte monolayer cultures. Ann Biomed Eng 29S1,S-28, 2001.
66.
Chahine NO, Wang CC-B, Kelly TN, Lai WM, Hung CT, Ateshian GA: The strain-softening of bovine articular cartilage under infinitesimal deformation in unconfined compression. Ann Biomed
Eng 29S1, S-31, 2001.
67.
Ateshian GA, Stark R, Mauck RL, Hung CT: Joint-specific articular surface molds for the production of anatomically shaped tissue-engineered cartilage constructs. Ann Biomed Eng 29S1, S-151, 2001.
68.
Hung CT, Palmer GD, Chao GP-H, LeRoux MA, Valhmu WB: Differential regulation of hypotonic and hypertonic loading by exon 1 of the human aggrecan gene in chondrocytes. Trans Orthop Res Soc
27:137, 2002.
69.
Mauck RL, Seyhan SL, Jamieson KV, Nicoll SB, Ateshian GA, Hung CT: Synergistic effects of growth factors and dynamic loading for cartilage tissue engineering. Trans Orthop Res Soc 27: 213,
2002.
70.
LeRoux MA, Chao GP-H, Palmer GD, Hung CT: Osmotic regulation of chondrocyte gene expression occurs independent of changes in cell morphology and size. Trans Orthop Res Soc 27: 384, 2002.
71.
Chao GP-H, Nicoll SB, Hung CT: Electric-field induced migration and orientation of ligament fibroblasts and chondrocytes. Trans Orthop Res Soc 27: 582, 2002.
72.
LeRoux MA, Raina P, Ateshian GA, Guo, XE, Hung CT: Novel composite chondrocyte-seeded agarose-bone constructs for in vitro chondrogenesis. Trans Orthop Res Soc 27: 469, 2002.
73.
Chahine NO, Wang CCB, Mason JH, Lai WM, Hung CT, Ateshian GA: The roles of osmotic swelling pressure and tension-compression nonlinearity on stress-strain responses of bovine articular cartilage.
Trans Orthop Res Soc 27: 83, 2002.
74.
Wang CCB, Chahine NO, Kelly TN, Hung CT, Ateshian GA: Optical measurements of anisotropic material properties of articular cartilage and determination of its material symmetry in compression.
Trans Orthop Res Soc 27: 394, 2002.
75.
Ateshian GA, Mauck RL, Stark R, Nicoll SB, Hung CT: Joint specific surface molds for articular cartilage tissue engineering. Trans Orthop Res Soc 27: 251, 2002.
76.
Takai E, Katz RW, Landesberg R, Hung CT, Guo XE: Adhesion strength, focal adhesion kinase activation, and cytoskeletal organization of osteoblasts on various substrates. Trans Orthop Res Soc
27: 535, 2002.
77.
Guo XE, Shyu J, Takai E, Costa KD, Hung CT: Substrates influence osteoblast elastic modulus measured by atomic force microscopy. Trans Orthop Res Soc 27: 521, 2002.
78.
Tang Z, Chao G, Tucay A, Takai E, Djukic D, Lind ML, Hung CT, Guo XE, West A, Osgood R,
Yardley JT: XYZ on a chip: nanoscale fabrication, fluidics, and optics directed toward applications within biology and medicine. NATO Organic Nanophotonics, Aix-en-Provence, France, August 2002.
79.
Chao PG-C, Angelini E, Tang Z, ChangW., Bulinski JC, West AC, Hung CT: Novel application of microfluidiic channels in studying cell migration and reorientation in response to direct current electric fields. IMECE2002-33138, 2002.
Clark T. Hung, Ph.D. 27.

80.
Nicoll SB, Mauck RL, Tsay RC, Hung CT, Ateshian GA: Intermittent hydrostatic pressurization modulates gene expression in human dermal fibroblasts seeded in 3D polymer scaffolds. IMECE2002-
33604, 2002.
81.
Takai E, Hung CT, Tucay A, Djukic D, Linde ML, Costa KD, Yardley J, Guo XE: Design of a microfluidic system for 3D culture of osteocytes in vitro. IMECE2002-33229, 2002.
82.
Takai E, Guo XE, Lu HH, LeRoux MA, Raina P, Ateshian GA, Hung CT: Strategy for tissue engineering of osteochondral constructs. IMECE2002-33595, 2002.
83.
Chahine NO, Wang CC-B, Hung CT, Ateshian GA., 2002, Determination of Poisson’s Ratios of
Bovine Articular Cartilage in Tension and Compression Using Osmotic and Mechanical Loading.
IMECE2002-32622
84.
Ateshian GA, Mauck RL, Hung CT: Modeling of neutral solute transport in a dynamically loaded porous permeable gel: implications for articular cartilage biosynthesis and tissue engineering. To be presented at the Symposium on the Mechanics of Physiochemical and Electromechanical Interactions in Porous Media, May 18-23, 2003, Rolduc, kerkrade, The Netherlands.
85.
Lu HH, Jiang J, Hung CT, Lima E: Characterization of a composite scaffold with intended application in osteochondral grafting. Musculoskeletal Biology & Bioengineering Gordon Conference, Proctor
Academy, Andover, New Hampshire (poster presentation), July 28-August 2, 2003
86.
Ateshian GA, Mauck RL, Wang CCB, Park S, Lima E, Lu HH, Chen H, Guo XE, Hung CT: The role of dynamic loading in cartilage functional tissue engineering. Presented at the Tissue Engineering
Conference, Cold Spring Harbor Laboratory, Long Island, NY, November 21-24, 2002.
87.
Takai E., Lima E, Lu HH, Ateshian GA, Guo XE, Hung CT: Devitalized bone as a mineralized substrate for osteochondral tissue engineered hydrogel constructs. Trans Orthop Res Soc 28: 307,
2003.
88.
Chahine NO, Wang CCB, Kelly TN, Elbeshbeshy R, Mauck RL, Hung CT, Ateshian GA: The effect of finite compressive strain on chondrocyte viability in statically loaded bovine articular cartilage.
Trans Orthop Res Soc 28: 646, 2003.
89.
Kim CH, Han SH, Chen FH, Ateshian GA, Hung CT, Guo XE: Intervertebral disc response to in vivo dynamic loading in a rat-tail model. Trans Orthop Res Soc 28: 57, 2003.
90.
Mauck RL, Hung CT, Ateshian GA: Modeling solute transport with mixture theory for dynamically loaded porous permeable gels. Trans Orthop Res Soc 28: 662, 2003.
91.
Ng, K, Wang CCB, Guo XE, Ateshian GA, Hung CT: Characterization of inhomogeneous bi-layered chondrocyte-seeded agarose constructs of differing agarose concentrations. Trans Orthop Res Soc 28:
960, 2003.
92.
Mauck RL, Lima EG, Oswald ES, Hung CT, Ateshian GA: Hydrostatic pressure increases matrix deposition and material properties of chondrocyte-seeded agarose hydrogels. 2003 Trans Orthop Res
Soc 28: 685, 2003.
93.
Nicoll SB, Mauck RL, Bordone LA, Tsay R, Hung CT, Ateshian GA: Hydrostatic pressure stimulates expression of fibrocartilaginous extracellular matrix proteins in human dermal fibroblasts seeded in 3-
D polymer scaffolds. Trans Orthop Res Soc 28: 950, 2003.
94.
Hung CT, Lima EG, Mauck RL, Lu HH, Ateshian GA: Considerations for the design of biphasic anatomically-shaped constructs for articular cartilage tissue-engineering. Trans Orthop Res Soc 28:
971, 2003.
95.
Kelly TN, Wang CCB, Mauck RL, Chahine NO, Ateshian GA, Hung CT: Effects of dynamic loading on material properties of chondron-seeded hydrogels. Trans Orthop Res Soc 28: 132, 2003.
96.
Mauck RL, Wang CCB, Cheng Q, Gabriel N, Oswald ES, Ateshian GA, Hung CT: Optimization of parameters for articular cartilage tissue engineering with deformational loading. Trans Orthop Res Soc
28: 305, 2003.
Clark T. Hung, Ph.D. 28.

97.
Chao PG, Han SH, Nicoll SB, Hung CT: ACL and MCL fibroblast migration in response to an applied
DC electric field. Trans Orthop Res Soc 28: 673,2003.
98.
Chao PG, Tang X, Angelini E, West AC, Hung CT: A novel microfluidic device to study the real-time response of cultured cells to applied dynamic osmotic loading. Trans Orthop Res Soc 28 : 252, 2003.
99.
Bear D, Warren C, Bui P, Chafik D, Hung CT, Gupta R: Schwann cells remain promyelinogenic after being subjected to sustained shear stress. Trans Orthop Res Soc 28: 96, 2003.
100.
Takai E, Kinnebrew GH, Hung CT, Guo XE: Co-culture of osteocytes and osteoblasts in a 3D trabecular bone explant. Trans Orthop Res Soc 28: 308, 2003.
101.
Ho MMY, Ng KW, Mauck RL, Ateshian GA, Hung CT: Gelling temperature and gel concentration effects on tissue development in chondrocyte-seeded agarose hydrogels. Adv Bioeng
(0355.pdf), 2003.
102.
Lima EG, Mauck RL, Park S, Gasinu S, Ng KW, Hung CT, Ateshian GA: Material properties of osteochondral constructs and biphasic finite element models of dynamic loading for articular cartilage tissue engineering.
Adv Bioeng (1129.pdf), 2003.
103.
Mauck RL, Ho MMY, Hung CT, Ateshian GA: Growth factor supplementation and dynamic hydrostatic pressurization for articular cartilage tissue engineering. Adv Bioeng (0283.pdf), 2003.
104.
Mauck RL, Wang CCB, Chen FH, Lu HH, Ateshian GA, Hung CT: Dynamic deformational loading of chondrocyte–seeded agarose hydrogels modulates deposition and structural organization of matrix constituents. Adv Bioeng (0531.pdf), 2003.
105.
Mauck RL, Oswald ES, Cheng Q, Majumdar MK, Nicoll SB, Ateshian GA, Hung CT: Hydrostatic pressure enhances chondrogenic differentiation of human mesenchymal stem cells in alginate disks.
Adv Bioeng (0265.pdf), 2003.
106.
Wang CCB, Kelly TN, Mauck RL, Ateshian GA, Hung CT: Temporal and spatial development of construct stiffness in chondrocyte-seeded agarose disks cultured in free-swelling and dynamically loaded configurations. Adv Bioeng (0279.pdf), 2003.
107.
Kelly TN, Wang CCB, Mauck RL, Ateshian GA, Hung CT: Effects of seeding density and native pericellular matrix on the response of chondrocytes to dynamic loading. Adv Bioeng (0187.pdf), 2003.
108.
Chao PHG, Tang Z, West AC, Hung CT: Chondrocyte size changes in response to cyclic hypertonic loading using a novel microfluidic device. Adv Bioeng (1127.pdf), 2003.
109.
Costa KD, Ho MMY, Hung CT: Multi-scale measurement of mechanical properties of soft samples with atomic force microscopy. Adv Bioeng (0285.pdf), 2003.
110.
Takai E, Mauck RL, Hung CT, Guo XE: Osteocyte interaction with osteoblasts and response to intermittent hydrostatic pressure loading in a 3D trabecular bone explant culture model. Adv Bioeng
(0183.pdf), 2003.
111.
Chahine NO, Wang CCB, Elbeshbeshy RR, Hung CT, Ateshian GA: Depth-dependent orthotropic tensile and compressive properties of human patellar cartilage. Adv Bioeng (0563.pdf), 2003.
112.
Chahine NO, Mantzaris AA, Chen FC, Hung CT, Ateshian GA: Direct measurement of osmotic pressure of glycosaminoglycan solutions. Adv Bioeng (1145.pdf), 2003.
113.
Park S, Krishnan R, Hung CT, Ateshian GA: In situ measurement of the dynamic modulus of bovine humeral head articular cartilage under physiological contact loading conditions. Adv Bioeng
(1207.pdf), 2003.
114.
Canal C, Meade NK, Park S, Wang CCB, Hung CT, Ateshian GA: Optical measurement of in situ strain fields within osteochondral tissue under indentation. Adv Bioeng (0363.pdf), 2003.
115.
Moa-Anderson BJ, Costa KD, Hung CT, Ateshian GA: Bovine articular cartilage surface topography and roughness in fresh versus frozen tissue samples using atomic force microscopy. Adv
Bioeng (0561.pdf), 2003.
Clark T. Hung, Ph.D. 29.

116.
Mauck RL, Ateshian GA, Hung CT: Theoretical considerations for functional cartilage tissue engineering. 3 rd International Symposium on Mechanobiology of Cartilage and Chondrocytes, May
17, 2003, Brussels, Belgium.
117.
Kelly TN, Oswald ES, Mauck RL, Ateshian GA, Hung CT: Reducing serum dependence: tissue engineering of cartilage in ITS supplemented media. Trans Orthop Res 29:725, 2004.
118.
Kelly TN, Ho MMY, Mauck RL, Ateshian GA, Hung CT: Effects of pre-elaborated ECM on development of functional properties in chondrocyte-seeded agarose constructs subjected to physiologic deformational loading. Trans Orthop Res 29:695, 2004.
119.
Lima EG, Mauck RL, Gasinu S, Ateshian GA, Hung CT: Functional tissue engineering of freeswelling and dynamically loaded osteochondral constructs. Trans Orthop Res 29:13, 2004.
120.
Chahine NO, DuBois CR, Ateshian GA, Hung CT: Optical determination of strain fields at the interface between graft and articular cartilage in an in vitro model of cartilage repair. Trans Orthop
Res 29:174, 2004.
121.
Mauck RL, Chahine NO, Lima EG, Hung CT, Ateshian GA: Functional tissue formation: from bulk properties to cellular microenvironments. Trans Orthop Res 29:309, 2004.
122.
Chao PHG, Cannon AM, Costa KD, Hung CT: Effects of basal osmolarity on chondrocyte transient behavior to step osmotic loading. Trans Orthop Res 29:538, 2004.
123.
Ng KW, Mauck RL, Ateshian GA, Hung CT: Dynamic loading modulates the inhomogeneity of bi-layered chondrocyte-seeded agarose constructs of differing agarose concentrations. Trans Orthop
Res 29:842, 2004.
124.
Han S, Ho MMY, Kim CH, Chen FH, Weidenbaum M, Ateshian GA, Hung CT, Guo XE: In vivo hyperphysiologic load at high frequencies is detrimental to intervertebral disc composition in rat tails.
Trans Orthop Res 29:22, 2004.
125.
Jiang J, Hung CT, Guo XE, Ateshian GA, Lu HH: Three dimensional degradable, bioactive polymer ceramic-hydrogel composite for osteochondral repair. Trans Orthop Res 29:15, 2004.
126.
Krishnan R, Caligaris M, Mauck RL, Hung CT, Costa KD, Ateshian GA: Removal of the superficial zone of bovine articular cartilage does not increase its frictional coefficient. Trans Orthop
Res 29:172, 2004.
127.
Cai W, Thomas AO, Hung CT, Chen FH: Role of COMP in chondrocyte ERK signaling pathway activation. Trans Orthop Res 29:594, 2004.
128.
Takai E, Hung CT, Costa KD, Yardley JT, Guo XE: Controlled culture of bone cellular networks in 2D and 3D. Trans Orthop Res 29:376, 2004.
129.
Takai E, Huang MS, Mauck RL, Hung CT, Guo XE: Osteocytes regulate osteoblast function in a
3D trabecular bone explant under dynamic hydrostatic pressure. Trans Orthop Res 29:90, 2004.
130.
Chahine NO, Chen FH, Hung CT, Ateshian GA: Direct measurement of the osmotic pressure of glycosaminoglycan solutions at room temperature in sodium chloride. Trans Orthop Res 29:524, 2004.
131.
Hung CT, Kelly TN, Lima EG, Ng KW, Ateshian GA: A Functional Tissue Engineering Strategy for Repair and Replacement of Osteoarthritic Cartilage, Presented at the European Society of
Biomechanics 2004, the Netherlands, July 4-7, 2004.
132.
Likhitpanichkul M, Chao PG, Hung CT, Ateshian GA: Determination of modulus and permeability of chondrocytes from cell response to osmotic loading. Presented at the European Society of Biomechanics 2004, the Netherlands, July 4-7, 2004.
133.
Chao PG, Navratil FR, Hung CT: Effects of basal osmolarity on chondrocyte cell size change and intracellular calcium response to step osmotic loading. Presented at the 2004 BMES Annual Meeting,
Philadelphia, PA, October 13-16.
Clark T. Hung, Ph.D. 30.

134.
Ng KW, Kelly TN, Chahine NO, Costa KD, Ateshian GA, Hung CT: Development of functional properties of bi-layered chondrocyte-seeded agarose constructs. Presented at the 2004 BMES Annual
Meeting, Philadelphia, PA, October 13-16.
135.
Hung CT, Lima EG, Ng KW, Kelly TN, Chahine NO, Mauck RL, Costa KD, Ateshian GA:
Applied deformational loading to promote/guide development of chondrocyte-seeded agarose constructs. Presented at the 2004 BMES Annual Meeting, Philadelphia, PA, October 13-16.
136.
Takai E, Mauck RL, Huang MS, Hung CT, Guo XE: Osteocyte and osteoblast response to dynamic hydrostatic pressure in a 3D trabecular explant. Presented at the 2004 BMES Annual
Meeting, Philadelphia, PA, October 13-16.
137.
Morrison B, Cater HL, Wang C, Davis S, Lennon J, Ateshian GA, Hung CT: A detailed mechanical tolerance criterion for living brain at the tissue level. Presented at the 2004 BMES Annual
Meeting, Philadelphia, PA, October 13-16.
138.
Fan C, Ho W, Chao PG, Hung CT, Morrison B: Osmotic loading of astrocytes: implications for post-traumatic edema. Presented at the 2004 BMES Annual Meeting, Philadelphia, PA, October 13-
16.
139.
Basalo IM, Ateshian GA, Hung CT: Effect of chondroitinase ABC on the equilibrium frictional properties of articular cartilage. Presented at the 2004 BMES Annual Meeting, Philadelphia, PA,
October 13-16.
140.
Moffat K.L., Chahine N.O., Hung C.T., Ateshian G.A., Lu H.H., 2005, Mechanical Properties of
Fibrocartilage At the ACL Insertion Site. International Symposium on Ligaments and Tendons-V .
Washington D.C.
141.
Gupta R. et al: Shear stress alters the expression of myelin associated glycoprotein and myelin basic protein in schwann cells. Trans Orthop Res Soc 30: 225, 2005
142.
Ho MM, Ng KW, Capiola D, Weidenbaum M, Ateshian GA, Guo XE, Hung CT: A rat-tail in vivo bioreactor for physiologic loading of engineered tissue constructs. Trans Orthop Res Soc 30: 261,
2005
143.
Ho MM, Ateshian GA, Hung CT: Determination of in situ strain fields in rat tail intervertebral disc under axial compressive loading. Trans Orthop Res Soc 30: 271, 2005.
144.
Ng KW, Saliman JD, Kelly TN, Statman LY, Ateshian GA, Hung CT: Collagen hydrolysate increases the mechanical properties and type II collagen synthesis of tissue engineered articular cartilage. Trans Orthop Res Soc 30: 259, 2005.
145.
Chao PG, Han S, Costa KD, Hung CT: Chondrocyte calcium signaling is modulated by dynamic osmotic loading. Trans Orthop Res Soc 30: 906, 2005.
146.
Cook JL, Lima EG, Hung CT, Ateshian GA, Kuroki K, Stoker AM, Fox DB: In vitro and in vivo evaluation of tissue-engineered constructs for articular cartilage regeneration. Trans Orthop Res Soc
30: 1767, 2005.
147.
Ho MM, Ng KW, Kelly TN, Ateshian GA, Kuroki K, Cook JL, Hung CT: Synoviocyte potential for articular cartilage tissue engineering and repair. Trans Orthop Res Soc 30: 1789, 2005.
148.
Ng KW, Saliman JD, Kelly TN, Statman LY, Ateshian GA, Hung CT: Hyaluronan modulates the material properties and biochemical content of dynamically-loaded chondrocyte-seeded agarose constructs. Trans Orthop Res Soc 30: 1787, 2005.
149.
Kelly TN, Ng KW, Lee EJ, Lima EG, Statman LY, Doty SB, Costa KD, Ateshian GA, Hung CT:
Development of radial properties of engineered articular cartilage using unconfined compression. .
Trans Orthop Res Soc 30: 1788, 2005.
150.
Han SH, Chao PG, Palmer GD, Hung CT: Influence of serum on hypotonic loading-induced chondrocyte mechanotransduction. Trans Orthop Res Soc 30: 1633, 2005.
Clark T. Hung, Ph.D. 31.

151.
Chahine NO, Lima EG, Victor W, Hung CT, Ateshian GA: Dynamic deformational loading significantly enhances the transport of dextran molecules into agarose hydrogels. Trans Orthop Res
Soc 30: 1791, 2005.
152.
Kelly TN, Fisher MB, Lima EG, Ateshian GA, Hung CT: Integrative properties of chondrocyteseeded agarose constructs. Trans Orthop Res Soc 30: 1790, 2005.
153.
Takai E, Hung CT, Costa KD, Guo XE: Substrate modulation of osteoblast modulus and response.
Trans Orthop Res Soc 30: 333, 2005
154.
Takai E, Xu Q, Jiang, XJ; Whitesides GM, Costa KD, Yardley JT, Hung CT, Guo XE : Calcium signaling in controlled 2-dimensional bone cell cultures. Trans Orthop Res Soc 30:56, 2005
155.
Chahine N.O., Lima E.G., Ateshian G.A., Hung C.T., 2005, Effect of Dynamic Loading on the
Transport of Solutes into Agarose Hydrogels, 4 th Annual International Symposium on
Mechanobiology of Cartilage and Chondrocyte, Budapest, Hungary.
156.
Albro MB, Chahine NO, Ng KW, Likhitpanichkul M, Hung CT, Ateshian GA: Osmotic loading of alginate gels: a biomimetic study of hindered transport in the cell cytoplasm. Proceedings of the 2005
Summer Bioengineering Conference, Vail, CO.
157.
Ho MM, Kelly TN, Ateshian GA, Hung CT: Rat tail intervertebral disc mechanical response with intact and excised nucleus pulposus . Proceedings of the 2005 Summer Bioengineering Conference ,
Vail, CO.
158.
Ng KW, Statman LY, Ateshian GA, Hung CT : Evaluation Of Hyclone Bovine Growth Serum For
Use In Cartilage Tissue Engineering.
Proceedings of the 2005 Summer Bioengineering Conference ,
Vail, CO.
159.
Chao PG, Hung CT: Effects of dynamic osmotic loading on chondrocyte calcium response and gene expression. Proceedings of the 2005 Summer Bioengineering Conference , Vail, CO.
160.
Chao PG, Hung CT: Effects of constant and pulsed direct current electric fields on ACL fibroblast migration and gene expression . Proceedings of the 2005 Summer Bioengineering Conference , Vail,
CO.
161.
Lima EG, Setti P, Ateshian GA, Cook JL, Cook CR, Hile DD, Hung CT: Tissue engineering of cylindrical and anatomically-shaped osteochondral constructs using poly (propylene glycol-co-fumaric acid) as a moldable, porous substrate. Proceedings of the 2005 Summer Bioengineering Conference ,
Vail, CO.
162.
Chahine NO, Chen FH, Hung CT, Ateshian GA: The contribution of osmotic pressure to the effective compressive aggregate modulus of bovine articular cartilage. Proceedings of the 2005
Summer Bioengineering Conference , Vail, CO.
163.
Chahine NO, Lima EG, Wei VI, Hung CT, Ateshian GA: Effect of simultaneous application of direct perfusion and dynamic loading on the transport of dextran into agarose hydrogels. Proceedings of the 2005 Summer Bioengineering Conference , Vail, CO.
164.
Kelly TN, Chahine NO, Fisher MB, Ng KW, Tai T, Ateshian GA, Hung CT: Tension-compression nonlinearity in chondrocyte-seeded agarose hydrogels. Proceedings of the 2005 Summer
Bioengineering Conference , Vail, CO.
165.
Oswald ES, Chao PG, Hung CT: The effects of osmotic loading on bovine chondrocyte and
BMSC cell shape change and intracellular calcium response. Proceedings of the 2005 Summer
Bioengineering Conference , Vail, CO.
166.
Moffat KL, Chahine NO, Hung CT, Ateshian GA, Lu HH: Characterization of the mechanical properties of the ACL-bone insertion site. Proceedings of the 2005 Summer Bioengineering
Conference , Vail, CO. (First Place in Master’s Poster Competition in Cell/Tissue Engineering
Category)
Clark T. Hung, Ph.D. 32.

167.
Hung CT, Kelly TN, Chahine N.O., Ateshian GA, 2005, Cartilage Tissue Engineering:
Implications of Applied Deformational Loading and Solute Transport , Annals of Biomedical
Engineering , Baltimore, MD, Abstract #142478.
168.
Lima EG, Tai T, Bian L, Ateshian GA, Hung CT: Physiologic deformational loading does not counteract the catabolic effects of interleukin-1B in cultured chondrocyte-seeded agarose constructs.
Trans Orthop Res Soc 31: 1455, 2006.
169.
Lima EG, Bian L, Serebrov A, Mauck RL, Byers B, Tuan R, Ateshian GA, Hung CT: Measuring the frictional properties of tissue engineered cartilage constructs. Trans Orthop Res Soc 31: 1501,
2006.
170.
Ho MM, Ng KW, Quinnan SM, Weidenbaum M, Ateshian GA, Guo XE, Hung CT: Cellular responses of tissue engineered constructs in a rat-tail in vivo bioreactor.
Trans Orthop Res Soc 31:
789, 2006.
171.
Kelly TN, Ng, KW, Tai T, Ateshian GA, Hung CT: Effects of low-serum and dynamic loading on chondrocyte-seeded constructs. Trans Orthop Res Soc 31: 790, 2006.
172.
Ng KW, Bian L, Lin EY, Kelly TN, Ateshian GA, Hung CT: Essential amino acids supplementation modulates chondrocyte behavior in 3D agarose culture.
Trans Orthop Res Soc 31:
791, 2006.
173.
Chahine NO, Wei VI, Lima EG, Albro MB, Hung CT, Ateshian GA: Effect of perfusion and dynamic loading on the distribution and content of solutes in patellar shaped agarose constructs. Trans
Orthop Res Soc 31: 792, 2006.
174.
Ng KW, Friedman DJ, Statman LY, Kelly TN, Ateshian GA, Hung CT: Cell seeding method influences tissue spatial development in chondrocyte-seeded gelatin scaffolds.
Trans Orthop Res Soc
31: 793, 2006.
175.
Chao PG, Oswald ES, Hung CT: Osmotic loading-induced volume and gene expression changes in chondrocytes involve calcium mobilization. Trans Orthop Res Soc 31: 1349, 2006.
176.
Chao PG, Bulinski JC, Ateshian GA, Hung CT: Effects of osmotic loading on chondrocyte nuclear size. Trans Orthop Res Soc 31: 134, 2006.
177.
Takai E, Xu Q, Jiang JI, Whitesides GM, Costa KD, Yardley JT, Hung CT, Guo XE: Role of cell separation distance on calcium signaling in controlled 2-dimenional bone cell networks. Trans Orthop
Res Soc 31: 23, 2006.
178.
Grayson WL, Chao PHG, Cannizzaro C, Hung CT, Vunjak-Novakovic G: Engineering human osteochondral grafts using spatially controlled multiparametric stimulation. Trans 2006 BMES ,
Chicago IL, in submission.
179.
Grayson WL, Cannizzaro C, Chao PHG, Hung CT, Vunjak-Novakovic G: Engineering an anatomically-shaped TMJ with human mesenchymal stem cells .
Musculoskeletal Biology &
Bioengineering Gordon Conference, Proctor Academy NH, July 2006.
180.
Chahine NO, Hung CT, Ateshian GA: ECM VII: Experimental and Finite Element Analysis of
Strain Fields in Chondrocytes and the Extracellular Matrix of Cartilage Under Physiological Loading.
Proceedings of the AO Research Foundation Conference , Davos, Switzerland, June 26-29, 2006.
181.
Chahine NO, Hung CT, Ateshian GA: In Situ Strain Measurments of Chondrocyte Deformation
Under Transient Loading. 2006 Summer Bioengineering Conference, Amelia Island FL, June 21-25,
2006.
182.
Chahine NO, Hung CT, Ateshian GA: Osmotic loading of alginate gels: a biomimetic study of hindered transport in the cell cytoplasm. 2006 Summer Bioengineering Conference, Amelia Island FL,
June 21-25, 2006.
Clark T. Hung, Ph.D. 33.

183.
Ng KW, Kugler L, Kelly TN, DeFrancis J, Ateshian GA, Hung CT: Enzymatic removal of agarose scaffold for tissue engineered cartilage. 2006 Summer Bioengineering Conference, Amelia Island FL,
June 21-25, 2006.
184.
Basalo I, Chahine NO, Kaplun M, Chen F, Hung CT, Ateshian GA: Chondroitin-sulfate reduces the friction coefficient of articular cartilage. 2006 Summer Bioengineering Conference, Amelia Island
FL, June 21-25, 2006.
185.
Chahine NO, Lima EG, Wei V, Albro M, Hung CT, Ateshian GA: Effect of direct perfusion and dynamic loading on solute transport in patellar shaped constructs. 2006 Summer Bioengineering
Conference, Amelia Island FL, June 21-25, 2006.
186.
Hung CT, Kelly TN, Lima EG, Bian L, Ateshian GA: Characterization of Chondrocyte-Seeded
Agarose Constructs Subjected to Physiologic Deformational Loading. Trans 2006 BMES , Chicago IL
187.
Oswald ES, Chao PHG, Bulinski JC, Ateshian GAA, Hung CT: Chondrocyte nuclear response to osmotic loading. Trans IEEE-EMBS Conference , New York, NY, September 2006.
188.
Lima EG, Bian L, Mauck RL, Byers BA, Tuan R, Ateshian GA, Hung CT: The effect of applied compressive loading on tissue-engineered cartilage constructs cultured with TGF-B3. Trans IEEE-
EMBS Conference , New York, NY, September 2006.
189.
Albro M, Chao PH, Hung CT, Ateshian GA: Partial volume recovery of chondrocytes upon osmotic loading explained by the cytoplasm’s passive steric exclusion of select solute species. Trans
Orthop Res Soc 32:151, 2007.
190.
Lima EG, Bian L, Yagoda D, Mauck RL, Byers RA, Tuan RS, Ateshian GA, Hung CT: Loading on tissue-engineered cartilage constructs cultured with TGF 3. Trans Orthop Res Soc 32:253, 2007.
191.
Lima EG, Tai T, Marra K, Ateshian GA, Hung CT: The use of genipin as a cross-linker to enhance the mechanical properties of tissue-engineered cartilage constructs. Trans Orthop Res Soc 32:351,
2007.
192.
Bian L, Lima EG, Angione S, Ng KW, Doty S, Ateshian GA, Hung CT: Efficacy of serum-free medium and dynamic loading in maintenance of long-term cultures of cartilage explants. Trans
Orthop Res Soc 32:569, 2007.
193.
Oswald ES, Chao PH, Cheng SL, Shin R, Bulinski JC, Ateshian GA, Hung CT: Zonal-dependent response of chondrocytes to osmotic loading.
Trans Orthop Res Soc 32:910, 2007.
194.
Bian L, Angione S, Ng KW, Lima EG, Ateshian GA, Hung CT: Influence of initial construct thickness on the material and biochemical properties of engineered cartilage. Trans Orthop Res Soc
32:1477, 2007.
195.
Kelly, TN, Ateshian GA, Hung CT: Effect of dynamic loading on tension-compression nonlinearity in engineered cartilage. Trans Orthop Res Soc 32:1478, 2007.
196.
Ng KW, Kugler L, Ateshian GA, Hung CT: Mechanical and biochemical properties of engineered chondrocyte-seeded hydrogel constructs recover over time in culture after enzymatic scaffold removal.
Trans Orthop Res Soc 32:1479, 2007.
197.
Bian L, Angione S, Lima EG, Ng KW, Ateshian GA, Hung CT: Tissue-engineered cartilage constructs using mature bovine chondrocytes: effects of temporal exposure to growth factors and dynamic deformational loading. Trans Orthop Res Soc 32:1480, 2007.
198.
Kelly TN, Ateshian GA, Hung CT: Radial development of material properties in chondrocyteseeded agarose hydrogels. Trans Orthop Res Soc 32:1481, 2007.
199.
Chao PH, Bhumiratana S, Lima EG, Grayson W, Hung CT, Vunjak-Novakovic G: Increased transport rates in a novel osteochondral bioreactor with simultaneous medium perfusion and dynamic loading. Trans Orthop Res Soc 32:1503, 2007.
Clark T. Hung, Ph.D. 34.

200.
Oswald ES, Chao PG, Bulinski JC, Ateshian GA, Hung CT: The role of microtubule organization in chondrocyte response to osmotic loading. Presented at the 2007 ASME Summer Bioengineering
Conference, Keystone, CO, June 19-24, 2007.
201.
Kugler L, Ng KW, O’Conor CJ, Ateshian GA, Hung CT: Scaffold properties play a critical role in the retention of synthesized glycosaminoglycans in tissue engineered cartilage. Presented at the 2007
ASME Summer Bioengineering Conference, Keystone, CO, June 19-24, 2007.
202.
O’Conor CJ, Ng KW, Kugler L, Ateshian GA, Hung CT: The response of tissue engineered cartilage to the temporal application of transforming and insulin-like growth factors. Presented at the
2007 ASME Summer Bioengineering Conference, Keystone, CO, June 19-24, 2007.
203.
Bian L, Ng KW, Lima EG, Ateshian GA, Hung CT: Influence of nutrient channels on development of engineered cartilage properties. Presented at the 2007 ASME Summer Bioengineering
Conference, Keystone, CO, June 19-24, 2007.
204.
Lima E, Bian L, Gonzales F, Ateshian GA, Hung CT: Influence of interleukin treatment on engineered and native articular cartilage. Presented at the 2007 ASME Summer Bioengineering
Conference, Keystone, CO, June 19-24, 2007. (Lima E: honorable mention PhD competition)
205.
Albro MB, Rajan V, Hung CT, Ateshian GA: Fickian behavior and concentration-dependence of the diffusion of dextran in agarose. Presented at the 2007 ASME Summer Bioengineering Conference,
Keystone, CO, June 19-24, 2007.
206.
Huo BB, Lu L, Costa KD, Hung CT, Guo XE: Mechanotransduction within bone cell network.
Presented at the 2007 Annual Meeting of the Biomedical Engineering Society, September 28, 2007.
207.
Lima EG, Stoker AM, Sohal J, Davis D, Ateshian GA, Cook JL, Hung CT: Beneficial effects of dexamethasone on the preservation of chondral allografts. Trans Orthop Res Soc 33:1060, 2008.
208.
Bian LM, Xu D, Mao DQ, Williams DY, Cook JL, Ateshian GA, Hung CT: Long-term preservation of chondral and osteochondral explants using tissue culture. Trans Orthop Res Soc
33:1058, 2008.
209.
Bian LM, Williams DY, Mao DQ, Xu D, Ateshian GA, Hung CT: Influence of temporary chondroitinase ABC-induced GAG suppression on maturation of tissue engineered cartilage. Trans
Orthop Res Soc 33:601, 2008.
210.
Ng KW, Ateshian GA, Hung CT: Layered hydrogel cartilage constructs using zonal chondrocytes.
Trans Orthop Res Soc 33:378, 2008.
211.
Ng KW, O’Conor CJ, Lima EG, Lo SB, Ateshian GA, Cook JL, Hung CT, Primed mature chondrocytes can develop an engineered cartilage with physiologic properties. Trans Orthop Res Soc
33:599, 2008.
212.
Ng KW, O’Conor CJ, Ateshian GA, Hung CT: The response of engineered cartilage to a timed application of transforming and insulin-like growth factors. Trans Orthop Res Soc 33:378, 2008.
213.
Oswald ES, Ateshian GA, Bulinski JC, Hung CT : Insensitivity of zonal chondrocyte proteoglycan synthesis to media osmolarity and sodium concentration in long-term culture. Trans Orthop Res Soc
33:1184, 2008.
214.
Chao PG, Yodmuang S, Grayson WL, Lima EG, Hung CT, Vunjak-Novakovic G: Inhibition of cartilage formation by decellularized bone: possible mechanisms. Trans Orthop Res Soc 33:575, 2008.
215.
Huo B, Lu X, Hung CT, Costa KD, Guo XE: Calcium response within bone cell network under fluid shear. Trans Orthop Res Soc 33:67, 2008.
216.
Canal C, Hung CT, Ateshian GA: Complete proteoglycan extraction from bovine articular cartilage reduces its compressive modulus more than twenty-fold.
Trans Orthop Res Soc 33:619,
2008.
Clark T. Hung, Ph.D. 35.

217.
Tan AR, Lima EG, Marra KG, Hung CT: Genipin protects engineered cartilage against IL-1alpha induced degradation. Proceedings of the 2008 Summer Bioengineering Conference, June 25-29, in
Marco Island, FL (Tan AR: 3 rd place in MSE competition)
218.
Bian L, Kelly TK, Lima EG, Ateshian GA, Hung CT: Finite element modeling of osmotic swelling in tissue-engineered cartilage. Proceedings of the 2008 Summer Bioengineering Conference, June 25-
29, in Marco Island, FL (Bian L: honorable mention PhD competition)
219.
Ng KW, Heitman DE, Ateshian GA, Cook JL, Hung CT: The response of adult engineered canine cartilage to the sequential or combined application of TGF-B3 and IGF1. Trans Orthop Res Soc
34:1017, 2009.
220.
Ng KW, Lima EG, Stoker AM, Kuroki K, Bal BS, Ateshian GA, Cook JL, Hung CT: In vivo response to canine chondral and osteochondral engineered cartilage implantation. Trans Orthop Res
Soc 34:60, 2009.
221.
Lima EG, Chao PH, Ateshian GA, Cook JL, Bal BS, Vunjak-Novakovic, Hung CT: Porous tantalum metal outperforms devitalized bone as a substrate for osteochondral tissue engineering. Trans
Orthop Res Soc 34:124, 2009.
222.
Tan AR, Dinh AR, Albro MB, Ateshian GA, Hung CT: Type IX agarose gel produces better tissue engineered cartilage constructs than Type VII Agarose. Trans Orthop Res Soc 34:1302, 2009.
223.
Tan AR, Barsi JM, Jayabalan PS, Rahaman MN, Bal BS, Ateshian GA, Cook JL, Hung CT: The potential for 13-93 bioglass as a medium supplement for culturing tissue engineered cartilage. Trans
Orthop Res Soc 34:1314, 2009.
224.
Tan AR, Ateshian GA, Hung CT: Chondrocyte death in overloaded engineered cartilage depends on construct maturity. Trans Orthop Res Soc 34:1090, 2009.
225.
Oswald ES, Bian L, Cook JL, Ateshian GA, Hung CT: Optimization of osteoarthritic chondrocytes as cartilage repair cells using physico-chemical techniques. Trans Orthop Res Soc 34:1229, 2009.
226.
Oswald ES, Ahmed HS, Ateshian GA, Hung CT: Extracellular osmotic and fixed charge density effects on chondrocyte proteoglycan replenishment after enzymatic digestion of cartilage explants.
Trans Orthop Res Soc 34:1045, 2009.
227.
Bian L, Oswald ES, Williams DY, Xu D, Ateshian GA, Marra KG, Hung CT: Chondrogenic induction of human adipose-derived stem cells in agarose hydrogel. Trans Orthop Res Soc 34:1301,
2009.
228.
Bian L, Xu D, Williams DY, Cook JL, Ateshian GA, Hung CT: Effects of dexamethasone on the functional properties of cartilage explants during long-term culture. Trans Orthop Res Soc 34:329,
2009.
229.
Wang IE, Kala S, Bian L, Hung CT, Lu HH: Fibroblast-osteoblast interactions and dynamic compression modulate regeneration of the ACL-bone interface. Trans Orthop Res Soc 34:1397, 2009.
230.
Albro MB, Li R, Yeager K, Hung CT, Ateshian GA: Dynamic loading of deformable porous media can induce active solute transport. Trans Orthop Res Soc 34:151, 2009.
231.
Cook JL, Lima EG, Ng KW, Kuroki K, Stoker AM, Bal BS, Ateshian GA, Hung CT: Towards biologic osteochondral resurfacing of the canine patella using tissue engineered anatomic constructs.
Trans Orthop Res Soc 34:1355, 2009.
232.
Hung CT, Bian L, Stoker A, Ateshian GA, Cook JL: Functional tissue engineering of articular cartilage using adult chondrocytes. Presented at the 2009 Annual Meeting of the International
Cartilage Repair Society, May 23-26, Miami, FL.
233.
Hung CT, Bian L, Lima E, Stoker A, Marberry KM, Ateshian GA, Cook JL: Tissue age-dependent dexamethasone modulation of cartilage properties in cartilage. Presented at the 2009 Annual Meeting of the International Cartilage Repair Society, May 23-26, Miami, FL.
Clark T. Hung, Ph.D. 36.

234.
Tan AR, Ateshian GA, Hung CT: Immediate and long-term response of engineered cartilage after mechanical injury. Trans Summer Bioengineering Conference, Lake Tahoe, CA, June 17-21, 2010
235.
Bian L, Stoker, AM, Lima EG, Ng KW, Ateshian GA, Cook JL, Hung CT: Dynamic deformational loading for cartilage functional tissue engineering. Trans Summer Bioengineering
Conference, Lake Tahoe, CA, June 17-21, 2010
236.
Dong EY*, Tan AR, Hung CT: The use of chondrocyte feeder layer enhances tissue engineered cartilage properties, REU papers, BMES annual meeting, Pittsburgh, PA, October 8-10, 2009. (*4 th place student award, REU oral presentations)
237.
Kramer SP, Oswald ES, Hung CT: Effects of extracellular osmotic environment on chondrocyte metabolic properties, REU papers, BMES annual meeting, Pittsburgh, PA, October 8-10, 2009.
238.
Ahmed HS, Oswald ES, Hung CT: Role of Na+/K+/2Cl- cotransporter in chondrocyte metabolic response to osmotic environment, REU papers, BMES annual meeting, Pittsburgh, PA, October 8-10,
2009.
239.
Khanarian N, Jiang J, McArdle S, Hung C, Lu H: Zonal chondrocyte interactions regulate chondrocyte calcification via PTHrP. Trans Orthop Res Soc 35:3, 2010.
240.
Durney K, Sirsi S, Nover A, Ateshian G, Konofagou E, Maleke C, Borden M, Lima E, Hung C:
Using microbubbles to modulate hydrogel scaffold properties for cartilage tissue engineering. Trans
Orthop Res Soc 35:122, 2010.
241.
Lee HG, Shin M, Kim KO, Patel N, Aydemir A, Mikami M, Minematsu H, Hung C, Lee F: Trans
Orthop Res Soc 35:382, 2010.
242.
Oswald E, Ahmed H, Bulinski C, Ateshian G, Hung C: Role of Na-K-Cl cotransporter in chondrocyte glycosaminoglycan production with long-term culture in varying osmolarities. Trans
Orthop Res Soc 35:874, 2010.
243.
Oswald E, Kramer S, Bulisnki C, Ateshian G, Hung C: The effect of osmolarity and salt concentration on zonal chondrocyte glycosaminoglycan production in long-term pellet culture. Trans
Orthop Res Soc 35:875, 2010.
244.
Albro M, Li R, Banerjee R, Chen B, Oungoulian S, de Palomar A, Trublja A, Hung C, Ateshian G:
Dynamic loading of immature cartilage pumps transferring out of blood vessel remnants and into the tissue matrix over short time sales. Trans Orthop Res Soc 35:912, 2010.
245.
Gunja N, Fong J, Vunjak-Novakovic G, Hung C: Migration responses of meniscus cells to applied direct current electric fields. Trans Orthop Res Soc 35:1051, 2010.
246.
Jayabalan P, Tan A, Rahaman M, Ball B, Sims H, Hung C, Cook J: Bioactive glass (13-93) as a suchondral substrate for tissue-engineered osteochondral constructs. Trans Orthop Res Soc 35:1236,
2010.
247.
Tan A, Rajan V, Ateshian G, Hung C: Reparative capacity of engineered cartilage to overloadinduced cracking or cutting injury in culture. Trans Orthop Res Soc 35:1331, 2010.
248.
Tan A, Dong E, Ateshian G, Hung C: Tissue engineered cartilage growth is expedited when cocultured with a chondrocyte feeder layer. Trans Orthop Res Soc 35:1333, 2010.
249.
Fong J, Gunja N, Kim, KO, Lee F, Hung C: Comparison of doxorubicin resistance of an osteosarcoma cell line in two and three dimensional cultures. Trans Orthop Res Soc 35:1646, 2010.
250.
Rajan V, Caligaris M, Hung C, Ahmad C, Ateshian G: Hemiarthroplasties defeat interstitial fluid pressurization in cartilage and promote greater friction than natural joints. Trans Orthop Res Soc 35:
2010.
251.
Gunja N, Fong J, Tan A, Moy M-Y, Xu D, O’Connell G, Bulinski JC, Ateshian GA, Hung CT:
Priming of synovium-derived mesenchymal stem cells for cartilage tissue engineering. Trans Summer
Bioengineering Conference, Naples, FL, June 16-19, 2010
Clark T. Hung, Ph.D. 37.

252.
Tan AR, Dong EY, Rho B, Sampat SR, Bulinski JC, Ateshian GA, Hung CT: Co-culture of a chondrocyte monolayer with engineered cartilage constructs immediately increases tissue properties.
Trans Summer Bioengineering Conference, Naples, FL, June 16-19, 2010
253.
Tan AR, Andry JP, Ateshian GA, Hung CT: Chondrogenic Media (CM) protects native and engineered cartilage from cell death after injury. Accepted to 9 in Sitges, Spain (in press). th World Congress of the ICRS meeting
254.
Sampat SR, Fong JV, O’Connell GD, Hung CT: Synovium-derived stem cells for cartilage tissue engineering. Presented at the BMES Annual Meeting, Austin, TX, October 6-10, 2010.
255.
Sampat SR, O’Connell GD, Fong JV, Ateshian GA, Hung CT: Optimization of synovium-derived stem cells for cartilage tissue engineering. Accepted to the Annual Meeting of the Orthopaedic
Research Society, Long Beach, CA, January 13-16, 2011.
256.
Dujari DN, Cunningham AS, Gunja NJ, Chen A, Bulinski JC, Ateshian GA, Hung CT: Directed synovium-derived stem cell migration in response to an applied DC electric field. Accepted to the
Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, January 13-16, 2011.
257.
Oswald ES, Brown LM, Bulinski JC, Marra KG, Lammi MJ, Hung CT: Differentiation-specific stem cell marker proteins detected by proteomics of adipose-derived human stem cells in hyperosmotic conditions. Accepted to the Annual Meeting of the Orthopaedic Research Society, Long
Beach, CA, January 13-16, 2011.
258.
Oswald ES, Stoker AM, Ahmed HA, Kramer SP, Bulinski JC, Ateshian GA, Hung CT: Effect of passaging and 3D culture osmotic conditions on osteoarthritic human chondrocyte GAG and
MMP/TIMP enzyme production. Accepted to the Annual Meeting of the Orthopaedic Research
Society, Long Beach, CA, January 13-16, 2011.
259.
Nover AB, O’Connell GD, Ateshian GA, Lima EG, Konofagou, EE, Hung CT: Focused ultrasound as a tool for modulating local tissue properties for articular cartilage tissue engineering.
Accepted to the Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, January 13-
16, 2011.
260.
Rho B, Bulinski JC, Ateshian GA, Hung CT: Passaging effects on chondrocyte and synoviumderived stem cell swelling response to hypo-osmotic loading. Accepted to the Annual Meeting of the
Orthopaedic Research Society, Long Beach, CA, January 13-16, 2011.
261.
Tan AR, Oswald ES, Hampilos PJ, Li VJ, Dowling RM, Bulinski JC, Liao JC, Hung CT,
Reprogramming adult bovine chondrocytes for cartilage tissue engineering. Accepted to the Annual
Meeting of the Orthopaedic Research Society, Long Beach, CA, January 13-16, 2011.
262.
Tan AR, Dowling RM, Andry JP, Yeager K, Ateshian GA, Cook JL, Hung CT: Optimizing osteochondral graft harvesting techniques to reduce cell death in surrounding tissue. Accepted to the
Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, January 13-16, 2011.
263.
Tan AR, Andry JP, Bulinski JC, Ateshian GA, Hung CT: Time-dependence of co-culture setup for expedited tissue engineered cartilage formation. Accepted to the Annual Meeting of the Orthopaedic
Research Society, Long Beach, CA, January 13-16, 2011.
264.
O’Connell GD, Fong JV, Joffe A, Newman IB, Hung CT: Trimethylamine N-oxide enhances the mechanical and biochemical properties of tissue engineered cartilage. Accepted to the Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, January 13-16, 2011.
265.
Durney KM, Sirsi SR, Nover A, Ateshian GA, Konofagou EE, Maleke C, Borden MA, Hung CT,
Lima EG: Microbubbles improve depth-dependent mechanical properties of cartilage tissue engineered constructs. Accepted to the Annual Meeting of the Orthopaedic Research Society, Long
Beach, CA, January 13-16, 2011.
Clark T. Hung, Ph.D. 38.

266.
Oungoulian S, Albro M, Saniel C, Bell F, Hung CT: Ateshian GA: The dose-dependent effect of
TGF-Beta on immature bovine articular cartilage properties during long term culture. Accepted to the
Annual Meeting of the Orthopaedic Research Society, Long Beach, CA, January 13-16, 2011.
267.
O’Connell GD, Collnick C, Ateshian GA, Bellamkonda, RV, Hung CT: Beneficial Effects of
Chondroitinase ABC Release From Lipid Microtubes Encapsulated in Chondrocyte-Seeded Hydrogel
Constructs. Presented at the 2011 ASME Summer Bioengineering Conference, SBC2011-53832,
Nemacolin Woodlands Resort, Farmington, PA.
268.
O’Connell GD, Hung CT, Ateshian GA: Experimental and theoretical evaluation of failure properties for immature tissue engineered cartilage. Presented at the 2011 ASME Summer
Bioengineering Conference, SBC2011-53385, Nemacolin Woodlands Resort, Farmington, PA.
269.
Tan AR, Alegre-Aguaron E, Dujari DN, Sampat SR, Bulinski JC, Ateshian GA, Hung CT: Effects of Passaging on the Migration Response of Synovium-Derived Stem Cells to an Applied DC Electric
Field. Presented at the 2011 ASME Summer Bioengineering Conference, SBC2011-53674,
Nemacolin Woodlands Resort, Farmington, PA.
270.
Nover AB, O’Connell GD, Ateshian GA, Lima EG, Konofagou EE, Hung CT: Effects of focused ultrasound on cell viability in its application to articular cartilage engineering. Transactions of the
2011 Annual Meeting of the Biomedical Engineering Society, Hartford, CT, October 12-15.
271.
O’Connell GD, Dunleavy N, Carapezza MA, Ateshian GA, Hung CT: Optimization of TMAO concentration for improved collagen production in engineered articular cartilage. Transactions of the
2011 Annual Meeting of the Biomedical Engineering Society, Hartford, CT, October 12-15.
272.
Alegre-Aguaron E, Sampat SR, Bulinski JC, Hung CT: Effects of cell priming on synovium derived stem cells for cartilage tissue engineering. Transactions of the 2011 Annual Meeting of the
Biomedical Engineering Society, Hartford, CT, October 12-15.
273.
Yuan X, Eng GM, Arkonac DE, Hung CT, Vunjak-Novakovic G: Endothelial cells enhance migration of meniscus cells in a three-dimensional hydrogel system. Transactions of the 2011 Annual
Meeting of the Biomedical Engineering Society, Hartford, CT, October 12-15.
PATENTS
1.
Hung CT, Ateshian GA, Lima EG, Cook JL, Bian L: Osteochondral implants, arthroplasty methods, devices, and systems, PCT application filed.
2.
Hung CT and O’Connell GD: Tissue culture media containing trimethylamine N-oxide, PCT application filed.
3.
Lima EG, Sirsi S, Hung CT, Borden MA: Gas-filled microbubbles devices and methods, PCT application filed.
4.
Cook JL, Hung CT, Stoker A, Lima EG: Chondral and osteochondral allograft preservation system, PCT application filed.
5.
Lu HH, Jiang J, Hung CT, Guo XE and Ateshian GA. “Polymer-ceramic-hydrogel composite scaffold for osteochondral repair,” patent pending.
ACTIVE GRANTS
1.
Principal Investigator: NIH: NIAMS 2R01AR46568-11: Physiologic loading for cartilage tissue engineering ; approximate yearly direct cost: $225,000, 9/1/09-8/31/13 (total cost for entire granting period: ~$2.38 million)- 20% yearly effort. Description: This COMPETING RENEWAL continues our investigation of the use of applied physiologic loading in a custom bioreactor to grow functional articular cartilage.
Clark T. Hung, Ph.D. 39.

Engineered constructs preconditioned with loading or free-swelling conditions will be implanted into a canine knee defect model with collaborators at the University of Missouri-Columbia (JL Cook, subcontract PI) to test the hypothesis that constructs cultured with physiologic loading will lead to better tissue repair. Complementary theoretical modeling of the biomechanical conditions at the defect site will be performed and (strain fields) validated using cadavers.
2.
Principal Investigator: NIH: NIAMS 2R01AR46568-09S1: Physiologic loading for cartilage tissue engineering ; approximate yearly direct cost: $65,000, 2/25/10-8/31/12 (total cost for entire granting period: $282,595). Description: Diversity supplement to support Dr. Grace. D. O’Connell.
3.
Investigator: NIH: NIBIB 5P41EB002520: Core- Tissue Engineering Bioreactor (Vunjak-
Novakovic, PI): 4% yearly effort, 9/1/09-8/31/14. Description: The focus of this core will be on the development and utilization of novel bioreactors designed to precisely control the cellular microenvironment, impart multiple physical stimuli, and enable real time imaging of cells and tissues at various hierarchical scales. Osteochondral (cartilage/bone) tissues and myocardium are selected as paradigms of distinctly different, clinically relevant engineered tissues to serve as models for bioreactor development and validation.
4.
Co-Investigator: NYSTEM ( PI: Mao JJ): Functional synovial joint replacement by stem cell homing;
2.5% effort, 1/1/09-12/31/11 (total cost: $1,079,986). Description: This project looks at strategies to optimize homing of stem cells for repair of the synovial joint using chemical growth factor release scaffolds.
5.
Co-Investigator:
NIH R21 EB11869-01A1
(PI: Vunjak-Novakovic G): Silk Hydrogel for Functional
Cartilage Tissue Engineering, 3/1/10-2/28/12; $432,046 (total costs); 5% effort. Description: Novel silk hydrogel scaffolds will be optimized to permit growth of functional cartilage. Solute diffusion, hydraulic permeability, equilibrium and dynamic modulus, and tissue biochemical composition will be evaluated.
6.
Co-Investigator: NIH: NIAMS R01 AR43628-14 (G Ateshian, PI): Biotribology of diarthrodial joints ; ($225,000 DC yearly)- 5/1/10-4/30/14- 10% yearly effort. Description: his grant represents a competing renewal that will test the hypothesis that standard hemiarthroplasties produce greater friction and wear on the native articular surface than a native joint.
7.
Principal Investigator: NIH AR46568-10S1 BIRT Supplement (7/1/10-8/31/12), direct costs:
$100,000, 2% yearly effort. Description: This proposal aims to include proteomics as a screening tool for optimization of cell passaging/priming for functional tissue engineering of cartilage.
8.
Co-Principal Investigator: NIH 1R01AR060361-01 (Ateshian GA, Contact PI): Optimizing nutrient supply in large engineered cartilage tissue constructs ($225,000 yearly DC: $1,742,366), 10% yearly effort- 9/20/10-8/31/15. Description: This grant examines the optimization and use of nutrient channels to cultivate large clinically relevant cartilage grafts.
9.
Co-Principal Investigator (Contact PI): NIH R21 EB014382-01A1 (M Borden, co-PI):
Microbubble-infused hydrogel for functional cartilage tissue engineering ($275,000 total DC), 2.5% yearly effort- 7/1/11-6/30/13. Description: This project merges microbubble technology to create micropores in hydrogel scaffolds that can aid nutrient access in growing engineered tissues by shortening the diffusion path length. Gasfilled microbubbles can be triggered to be filled by fluid.
10.
Co-Principal Investigator (contact PI: Kaplan, David with Levin, Michael and Vunjak-Novakovic,
Gordana): NIH 1R01AR061988-01: Electrotherapeutic Strategies for Connective Tissue Repair
($3,650,242 total costs). 7.5% yearly effort- 7/1/2011-6/30/2016. Description: Three specific aims will be pursued: (a) Biophysical regulation of chondrogenesis and osteogenesis in adult human stem cells, (b) Electrotherapeutic bioreactor models for regeneration of cartilage/bone tissues, and (c) Animal studies of cartilage/bone regeneration. The main technological impact will be the development of a new generation of electrotherapeutic medical devices termed
BioDomes.
Clark T. Hung, Ph.D. 40.

GRANTS PENDING
1.
Co-Principal Investigator (Contact PI): 1R21AR060502-A1 (E Konofagou, co-PI): HMIFU for osteochondral tissue engineering ($275,000 total DC), 5% yearly effort- 7/1/11-6/30/13. Description:
This project uses ultrasound technology as a non-invasive technique to create sealed interfaces between the cartilage and bone regions of engineered osteochondral grafts. Harmonic motion imaging for focused ultrasound permits ablation of tissue regions (at desired axial depths) with real-time monitoring of tissue mechanical property changes. (priority score: 12 percentile).
2.
Co-Principal Investigator (Contact PI: Hung; co-PIs: Vunjak-Novakovic G and Cook JL):
1R01AR062440-01: Cytokine Preconditioning for Cartilage Tissue Engineering (~$450,000/year)
7.5% yearly effort- 12/1/11-11/30/16. Description: This proposal examines the effect of cytokine pre-culture of engineered cartilage on its repair capacity in an in vitro model of OA (press fit into a cartilage ring, and subjected to dynamic loading, cytokine and co-culture with synovium). The in vitro studies will be used to optimize cytokine preconditioned constructs for implantation in an in vivo model of cartilage repair- full thickness defects in the canine stifle joint.
3.
Co-Principal Investigator (Contact PI: Hung; co-PIs: Chloe Bulinski and Jung-Chi Liao): Helmsley
Grant (Columbia University-Salk Institute). Project Title: Chondrocytic Differentiation and Cartilage
Repair Using Optimized Adipose-Derived Stem Cells, ($170,000 total costs), 11/1/11-10/31/13.
Description: This grant will in Aim 1 optimize and characterize chondrogenic potential of populations of canine and human adipose-dervied stem cells (ADSCs). Aim 2 will assess capacity of engineered cartilage using optimized ADSCs for cartilage repair.
PAST GRANTS
1.
Principal Investigator: (subcontract from University of Wisconsin) NIH: NIAMS R01 AR45753
(Wilmot Valhmu PI): Flow-induced MAPK and calcium signaling in chondrocytes ); approximate yearly direct cost: $87,262, 4/1/00-8/30/02 (co-PI of original grant received 9/98), (total cost for entire granting period: $115,090)- 25% academic and 33% summer effort. Description: The aim of this grant is to elucidate the role of mitogen activated protein kinases and calcium in chondrocyte mechanotransduction to fluid-induced shear stress. A major component of the grant is to delineate between concomitant stimuli that exist in the flow studies (e.g., electrokinetic phenomena, transport, shear rate).
2.
Principal Investigator: Whitaker Biomedical Research Grant: Shape changes and directed migration in chondrocytes to DC electric field s; approximate yearly direct cost:$70,000, 5/1/99-4/30/02 (total cost for entire period: $209,999)- 20% annual effort. Description: This grant examines the mechanism behind chondrocyte galvanotropism and galvanotaxis, shape change and directed migration in response to applied direct current electric fields. Studies include the examination of cell surface charge, cell-substrate interactions, and nature of the applied electric field stimulus.
3.
Principal Investigator: (subcontract from Conversion Energy Enterprises, Spring Valley, NY; SBIR
Phase I) NIH: NIAMS 1R43AR48738-01 (Soltz PI): Laser-assisted cartilage repair using collagen adhesives; $30,000 total cost, 5/15/02-10/15/02- 10% effort.
Description: The aim of this grant is to investigate the efficacy of laser activated collagen formulations as an adhesive and as an acellular graft that permits and/or promotes cell invasion.
Clark T. Hung, Ph.D. 41.

4.
Principal Investigator: Whitaker Foundation : Transitional Funding: Microfluidic device for osmotic loading of cultured chondrocytes ($80,000 total cost), 5/1/02-4/30/03, 20% yearly effort. Description:
The aim of this grant is to develop a microfluidic device capable of subjecting cultured chondrocytes to time-varying osmotic loading while permitting study of their biological response (e.g., cell shape and volume change, aggrecan gene expression).
5.
Principal Investigator: NIH: NIAMS 1R01AR46568-04 Supplement : approximate yearly direct cost:$29,000, 1/1/00-12/31/03 (total cost for entire granting period: $157,519) Description: Minority supplement for Terri-Ann Kelly (GRA) to expand the aims of the parent grant to study the development of inhomogeneous material and biochemical composition of chondrocyte-seeded agarose disks subjected to physiologic loading, using a microscopy-based system to measure tissue strains using a digital image correlation technique.
6.
Co-Investigator: NIH: NIAMS 1R01AR46532-04 (Gerard Ateshian PI): Anisotropy and nonlinearity of cartilage mechanics ; approximate yearly direct cost:$124,711, 2/1/00-1/31/03 (total cost for entire granting period: $724,337)- 20% academic and 30% summer effort. Description: This aim of this grant is to develop a model for articular cartilage that can explain its anisotropic and nonlinear behavior (in tension and compression).
Dr. Hung plays a role primarily in the measurement of cartilage material properties and tissue inhomogeneity using custom loading devices mounted on an epifluorescence microscopy system.
7.
Co-Investigator/Consultant: The Aircast Foundation (Ranjan Gupta PI: Schwann cell response to the biomechanical forces associated with carpal tunnel syndrome , $50,000/year, 6/03-5/05- 5% effort year 1. Description: This grant, awarded to Dr. Ranjan Gupta at UC Irvine, investigates the Schwann cell response to a reproducible, quantifiable mechanical stimuli in a controlled, physiologic environment. These studies will utilize parallel plate flow chambers and use various strategies to delineate between concomitant flow related stimulus in regulation of promyelogenic markers (MBP, MAG) as well as iNOS and VEGF mRNA.
8.
Principal Investigator: NIH administrative supplement for nano-scale research related to NIAMS
1R21 AR48791-03. Supplement for incorporation of laser tweezers ($50,000 total cost), 9/30/03-
9/29/04. Supplement to establish research collaboration with Dr. Michael Sheetz, Department of Biological Sciences at
Columbia University, to incorporate laser tweezers and magnetic bead pulling system to the project.
9.
Principal Investigator: National Institutes of Health (NIH): National Institutes of Arthritis,
Musculoskeletal and Skin Diseases (NIAMS) 1R01AR46568-05: Physiologic loading for cartilage tissue engineering ; approximate yearly direct cost:$143,890, 1/1/00-12/31/04 (total cost for entire granting period: $831,444)- 20% academic and 33% summer effort. Description: This grant investigates the use of applied physiologic loading in a custom bioreactor to grow functional articular cartilage. Bovine chondrocytes are encapsulated in agarose hydrogels and subjected to physiologic loading in long-term cultures. Tissue material properties and biochemical composition are measured and compared to those of native cartilage. (no cost extension)
10.
Principal Investigator: NIH: NIAMS 1R21 AR48791-03: Novel Determination of chondrocyte material properties; $75,000 yearly direct cost ($350,762 total cost), 7/1/02-6/30/0 5- 10% yearly effort. Description: The aim of this grant is to develop novel techniques to determine cell material properties using osmotic loading, videomicroscopy, Atomic Force Microscopy, and theoretical and computational modeling.
11.
Principal Investigator: NIH: NIAMS 1R01AR49922-03 Supplement: approximate yearly direct cost:$43,000, 1/1/04-6/30/05 (total cost for entire granting period: $86,285) Description: Minority supplement for Terri-Ann Kelly (GRA) to expand the aims of the parent grant to study the inhomogeneous material and biochemical composition of rat IVD subjected to well-controlled loading regimens in an in vivo rat-tail model.
12.
Co-Principal Investigator: National Science Foundation (NSF) MCB-0423475 (J. Chloe Bulinski,
PI): Probing mechanisms of cell motility in electric fields (total cost: $350,001)- 8/1/04-7/31/06- one month summer support. Description: This grant will investigate mechanisms (e.g., cell surface charge, enzymatic
Clark T. Hung, Ph.D. 42.

treatments, polycation and polyanions, cytoskeleton) on 3T3 Fibroblast orientation and directed migration to DC electric fields, in sparse and wounded monolayer cultures. Bioengineering analysis and microfluidic systems will be applied.
13.
Principal Investigator: NIH: NIAMS 1R01 AR49922-04: Intervertebral disc response to cyclic loading in vivo , $164,500 yearly direct cost ($1,041,497 total cost), 9/26/02-8/31/06, 20% yearly effort. Description: The aim of this grant is to investigate the material and biochemical properties (e.g., aggrecan, collagen and cartilage oligomeric matrix protein) of the intervertebral disc subjected to well-controlled loading regimens in an in vivo rat-tail model.
14.
Co-Investigator: NIH R21 AR052417-02A1 (Edward Guo, PI): Microfluidics and Bone
Mechanotransduction ; granting period: $275,000 total direct costs- 10% effort, 1/01/06-12/31/07.
Description: Use microfluidic technology to study osteocyte and osteoblast mechanotransduction to mechanical stimuli in a
2D and 3D model system. The role of cell-cell communication will be examined with respect to gap junction role in mediating intracellular calcium transients measured using fluorescence microscopy.
15.
Principal Investigator: Musculoskeletal Transplant Foundation (MTF) CU07-194: Loading for
Long-Term Maintenance of Osteochondral Grafts in Culture , 2% yearly effort, 1/1/08-7/30/09,
$125,000 total costs. Description: Exploration of physiologic loading bioreactors and serum-free culture media as a tissue culture technique for preservation of osteochondral grafts using canine tissue. Subcontract to James L. Cook, M.D.,
Ph.D., Comparative Orthopaedic Laboratory, University of Missouri, MO.
16.
Co-Investigator: NIH: NIAMS R01 AR43628-13 (Ateshian, PI): Biotribology of diarthrodial joints ;
(total cost: $1,766,739)- 6/1/04-4/30/09- 10% yearly effort. This grant represents a competing renewal that will use AFM, TIRF and custom testing devices to assess the role of the superficial zone and potential lubricants (such as lubricin or SZP protein) on diarthrodial joint lubrication.
17.
Consortium Principal Investigator: NIH 1R21AR053530-02 subcontract from University of
Missouri (James L. Cook, PI): Tissue engineered osteochondral patella resurfacing ; total costs:
$420,323 (total subcontract amount $165,000)- 2.5% effort, 3/1/06-2/28/09. Description: This application seeks to fabricate a tissue engineered osteochondral patella construct for implantation in a canine in vivo model. The
Columbia subcontract will take canine cells from the Comparative Orthopaedic Laboratory and culture osteochondral patella constructs that will subsequently be implanted into canine knees in Missouri.
18.
Co-Investigator: NYSTEM (PI: Vunjak-Novakovic G): Major equipment grant (Morningside
Heights); 4/1/08-3/31/09 (total costs $1,411,000): Functional imaging core for stem cells and tissue engineering.
Equipment includes a dual-photon confocal microscopy system, live animal imaging system, automated histology device and mass spectrometer for proteomics.
19.
Principal Investigator: NIH: NIAMS 2R01AR46568-08: Physiologic loading for cartilage tissue engineering ; approximate yearly direct cost: $200,000, 4/1/05-12/31/09 (total cost for entire granting period: $1,200,000)- 25% yearly effort. Description: This COMPETING RENEWAL continues our investigation of the use of applied physiologic loading in a custom bioreactor to grow functional articular cartilage. Bovine/canine chondrocytes are encapsulated in agarose hydrogels and subjected to physiologic loading in long-term cultures. Tissue material properties and biochemical composition are measured and compared to those of native cartilage. The aims of the grant are extended to study the effects of combined deformational loading with direct media perfusion, as well as the effects of loading boundary conditions on the development of tissue inhomogeneity and surface properties.
20.
Co-Investigator: NIH: NIAMS 1R01AR46532-09 (Ateshian PI): Anisotropy and nonlinearity of cartilage mechanics ; approximate yearly direct cost: $200,000, 2/1/04-1/31/10 (total cost for entire granting period: $1,571,996)- 20% yearly effort. Description: This COMPETING RENEWAL continues to to develop a model for articular cartilage that can explain its anisotropic and nonlinear behavior (in tension and compression).
It proposes a hierarchial series of progressively more complex experiments from the joint to the cellular level. Dr. Hung
Clark T. Hung, Ph.D. 43.

plays a role primarily in the measurement of cartilage material properties and tissue inhomogeneity, and local strain environment around the cell using custom loading devices mounted on an epifluorescence microscopy system.
21.
Co-Principal Investigator: NYSTEM (PIs: Vunjak-Novakovic G, Kalderon D, Bulinski JC):
Molecular, genetic and biophysical regulation of human stem cells for medical impact (consortium planning grant- Morningside Campus); 2.5% effort, 11/1/08-10/31/10 (total cost: $120,000)- no cost extension. Description: This grant supports a stem cell seminar series that is incorporated into a course offered to graduate students in biology and biomedical engineering.
22.
Principal Investigator : NIH 1S10RR027943-01: Columbia AFM Equipment Grant (total costs
$496,867, 4/22/10-4/21/11). Description: This proposal requests funds for a Veeco BioScope Catalyst atomic force microscope (AFM) system mounted on an inverted Olympus IX81 microscope equipped with a FluoView FV1000 laser scanning confocal microscopy system (LSCM).
23.
Principal Investigator: NIH: NIAMS 1R01AR052871-04A1: Chondrocyte mechanotransduction using microfluidics ; 25% yearly effort; approximate yearly direct costs: $200,000, 8/1/06-7/30/11
(total cost for entire granting period: $1,275,000). Description: This grant will study the dynamic osmotic loading response of cultured chondrocytes using a custom microfluidics device. The role of cell size change and osmotic load magnitude/frequency on the intracellular calcium signaling response and subsequent cytoskeletal rearrangement and aggrecan gene expression of chondrocytes will be assessed.
OTHER
Date of Birth:
Marital Status:
Hobbies:
May 18, 1968; Springfield, Massachusetts, USA
Spouse: Luci Morrone, M.D., M.P.H. (Pediatrician)
Tennis, Alpine Skiing, Piano/Keyboards
Clark T. Hung, Ph.D. 44.