Whitefly Sampling Scheme

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Estimate whitefly field populations1
A sampling scheme has been developed for sweet potato whiteflies, Bemisia
tabaci, on melons that may have some utility when trying to estimate
population levels in other crops. This is coupled with an action threshold.
This research was completed in response to some growers and pest control
advisors making control decisions based on information from sticky trap
counts. Sticky traps were found not to be highly correlated with actual
numbers of whiteflies found in fields. This is in part due to a redistribution
of populations following pesticide applications.
Visual observations of adults on leaves provide the most accurate
information and a practical method for estimating whitefly population levels.
The following describes a presence-absence sampling scheme. Presenceabsence sampling involves counting the number of leaves with one or more
whitefly adults, i.e., are they there (present) or not (absent). The actual
number of whiteflies on a leaf is unimportant. These numbers are related to
the mean number of whiteflies per leaf across the field. For example we
recommended that a grower sample 200 leaves in a field (50 for each
quadrant). If he finds 61% of the leaves have at least one whitefly then he
will know that the estimated infestation for the entire field is two to three
whiteflies per leaf (Table 1). This estimate is based on a 200-leaf sample;
reduction in sample size will reduce the accuracy of the estimation. Three
whiteflies per leaf is a suggested action threshold.
Table 1. Adjusted mean numbers of sweet potato whiteflies
per leaf corresponding to the proportion of infested leaves.
1
Proportion of infested
leaves
Estimated field population
01 to 09%
10 to 20%
21-30%
31 to 40%
41 to 50%
51 to 60%
61 to 70%
<1
<1
<1
<1
<1 to 1
1 to 2
2 to 3
From Palumbo, J. C., A. Tonhasca, and D. N. Byrne. 1994. Sampling plans and action
thresholds for whiteflies on spring melons. Cooperative Extension, University of
Arizona, IPM Series, No. 1.
71 to 80%
81 to 90%
91 to 100%
3 to 4
4 to 7
>7
The presence-absence sampling scheme for adults should not preclude
monitoring the crown portion of the plant for red-eyed nymphs. Presence of
these nymphs may indicate that adult numbers may rapidly increase in the
near future.
References
Tonhasca, A., J. C. Palumbo and D. N. Byrne. 1994. Aggregation patterns of
Bemisia tabaci in response to insecticide applications. Entomologia
Experimentalis et Applicata 72: 265-272.
Tonhasca, A., J. C. Palumbo and D. N. Byrne. 1994. Binomial sampling
plans for estimating Bemisia tabaci populations in cantaloupes.
Researches on Population Ecology 36: 181-186.
Palumbo J. C., A. Tonhasca and D. N. Byrne. 1995. Evaluation of three
sampling methods for estimating adult sweetpotato whitefly (Homoptera:
Aleyrodidae) abundance on cantaloupes. Journal of Economic
Entomology 88: 1393-1400.
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