USAFSAM
IMMUNODIAGNOSTICS
LABORATORY
25 March 2010
Presenters: SSgt Shoup, SSgt Eccleston
Distribution Statement A: Approved for Public Release; distribution is unlimited. 311ABG/PA No. 10-083, 12MAR2010
“We’re Moving – Not Closing”
Departments of Epidemiology Laboratory
First to move – Immunodiagnostics
Sections of Immunodiagnostics
Phasing of personnel and instrumentation
Modular facility for testing
Preparations both at Brooks City-Base and at
Wright-Patterson AFB
Transition of testing
The effects of moving in relation to customer
care – minimal 
ADVON team
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Brooks City-Base Lab
Departments of the Epi Laboratory
Immunodiagnostics
Microbiology
Virology
Bacteriology
Parasitology
Molecular
Shipping/Receiving
Customer Service
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Immunodiagnostics
Largest department in the Epi Laboratory
Performs over 90% of patient workload
Chosen to relocate first due to size of
instrumentation and workload volume
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PHE FY 2009 WORKLOAD
Total Tests: 2,039,587
Immunodiagnostics Tests: 1,896,006
Other PHE Tests: 143,581
Sections of Immunodiagnostics
HIV
HEPATITIS
EIA
BIOPLEX
IFA
GC/CHLAMYDIA
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Sections of Immunodiagnostics
HIV
Automated testing performed on
Siemens Centaur XP analyzer: HIV
Screens
Semi-Automated testing performed
using BioRad AutoBlot 3000: HIV
Western Blots
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Sections of Immunodiagnostics
Hepatitis
Automated testing performed on
Siemens Centaur Classics: Hepatitis
(A, B, C) Panels
Reflex testing for Hepatitis B
positive results
Manual HBeAg/HBeAb ELISA
testing
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Siemens Centaur Classic/XP
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BioRad AutoBlot 3000
Semi-Automated Western Blot analyzer
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Sections of Immunodiagnostics
Enzyme Immunoassay (EIA)
Tests performed on Trinity-Biotech
DSX analyzer: CMV G/M, H. Pylori,
Lyme AB, Rubeola, Mumps,
Varicella
Tests performed on BioRad Bioplex
analyzer: ANA, EBV G/M, HSV I/II,
Syphilis
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Trinity DSX and BioRad Bioplex
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Sections of Immunodiagnostics
Immunofluorescent Assay (IFA)
Confirmatory methods performed:
CMV M and WB Lyme M
Manual tests performed: AutoAntibody, Coccidioides, FTA-ABS,
Mycoplasma, Q Fever G/M, Rickettsia,
VDRL
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Sections of Immunodiagnostics
Miscellaneous Testing
Automated testing performed on
Gen-Probe Tigris analyzer:
Gonorrhea/Chlamydia
Automated testing performed on
Siemens Centaur XP analyzer:
Rubella G/M, Toxoplasma, Thyroid
AB
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Gen-Probe Tigris
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BRAC Personnel
Movement of military and civilian personnel
in 3 phases
Phase 1– ADVON team 6 mil, 2 civ PCS June
2010, Lab Director PCS Oct 2010
Phase 2– 3 mil, 2 civ PCS December 2010
Phase 3– 2 civ PCS January 2011
TDY– 2 civ for validation studies on
instrumentation
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BRAC Analyzers
Movement of instrumentation in 2
phases
Phase 1 – July 2010

2 Bioplex analyzers (1 from BCB to
WP,1 new one from BioRad)
 2 Tigris analyzers – brand new from
GenProbe
 4 Centaur analyzers – brand new from
Siemens
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BRAC Analyzers
Movement of instrumentation in 2
phases
Phase 2 – Jan 2011
 1 Bioplex analyzer (BCB)
 1 Tigris analyzer (BCB)
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Preparations while at BCB
Upfront plans completed prior to
move
Interop CHCS test files
Contracts – Equipment (new and reagent
rental, Personnel, Supplies)
Accreditations (CAP, CDC, etc.)
OIs/SOPs, Policies, Procedures
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Preparation for Testing at WP
Moving trucks arrive Jul 2010
Unpacking/Laboratory Set-up
Computer set-up/access
Vendors and analyzers
Validation
Vendors
Technical Supervisors
Initial testing scheduled to begin Oct
2010
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Testing Samples
Transition of patient samples to WP
Timeline
30% – 15 Oct 2010
60% - 15 Nov 2010
100% - 15 Dec 2010
Volunteers
First come, first serve!
CAP Inspection
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Streamlined Process
Dual-Operations
Full transition to Wright-Patterson
6 month process
Turn-Around Times
Maintained
Modular Bldg
18-month contract – Jan 2012
Transition to new facility after everyone
else
Instrumentation
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Streamlined Process
Modular
USAFSAM
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Summary
Breakdown of the Epi labs departments
and sections
Analyzers used for testing
Transition of personnel and equipment
to Wright-Patterson
Testing samples and streamlining the
ability to perform dual-operations
Your ADVON team members
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ADVON Team Members
Back: TSgt Mario Sulit, SSgt Andrew Eccleston, SSgt LeVaughn
Grant, Clay Huddleston
Front: Lt Col Kathy Taylor, SSgt Jennifer Shoup, Annette Compton,
SSgt Robert Bennett, Elsie Martinez
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Questions?
United States School of Aerospace
Medicine
Epidemiology Laboratory Service
(USAFSAM/PHE)
2730 Louis Bauer Drive
Brooks City-Base, TX 78235-5132
EPI Lab Customer Service Team
DSN: 240-8378
Commercial: 210.536.8378
Email: epilab.help@brooks.af.mil
Website: https://kx.afms.mil/epi/
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