Improvement of Rainfed Lowland Rice Variety
for Cooking Quality Traits using MAS
Sureeporn Katengam
Uraiwan Kotchasatit
UBONRATCHATHANI, THAILAND
Background



Rice production in NE of Thailand
Area of production ranging from
drought-prone lands to flooding area
Abiotic stresses related with
unpredictable rainfall pattern are main
problems
State of problem


Abiotic stresses associated with
unpredictable rainfall pattern are main
problems for rice production in NE of
Thailand.
Elite cultivars resistance to drought are
required.
State of problem (cont.)



KDML105, an aromatic rice with good cooking
quality, is widely grown in NE, however, its
production is dominating constraint with the
drought problem.
Drought tolerance is a complex traits since
this trait is governed by many QTLs locating
in many chromosome (ch1, ch2, ch3, ch4,
ch7, ch8,and ch9).
Therefore, improvement KDML105 for
drought resistance is not an easy tasks.
State of problem (cont.)


In the opposite way, improving of well adapted
variety to drought prone areas for other quality
traits such as cooking quality is worth to
reconsider.
Cooking quality trait is determined by three
characters specifying the physical properties of
starch in the endosperm:



Amylose content (AC)
Gel consistency (GC)
Gelatinization temperature (GT)
Objectives


Marker-assisted backcross breeding is aimed
at improving well drought adapted variety to
rainfed lowland area of NE of Thailand for
cooking quality traits utilizing tightly linked
DNA markers.
The major QTL controlling AC, GC, and GT
are located on Ch.6. DNA marker of flanking
markers closely linked to this major QTL
controlling quality traits will be carried out for
MAS.
Plant materials

Donor parent:

KDML105




low AC (~16-17%): major QTL on ch6 (at Wx gene )
low GT: major QTL on ch6
medium GC: major QTL on ch6 (at Wx gene )
Recipient parent:

IR57514-drought
Breeding scheme:
Genetic map (Azu / IR64 )
Chr.6
Chr.11
Map location of QTLs controlling cooking quality traits
Survey of SSR markers for parent screening
Expected size (bp)
#
Markers Traits
Chrom KDML 105 IR57514 KD571-77Size expected Remark
1
BADH
Aroma
8
~ 400
~410
~400
Polymorphic
2
RM190
Amylose content
6
~120
~116
~120
3
Waxy
& Gel consistency
6
~311
~315
~311
4
RM204
6
~155
~119
~155
5
RM205
6
121
121
121
6
7
RM225
RM111
Gelatinization
6
6
~145
120
~120
120
~145
120
140
124
Polymorphic
Monomorphic
8
RM50
temperature
6
200
200
200
201
Monomorphic
9
RM121
6
~275
~272
~275
Polymorphic
10
PSS-1-1
6
149
149
149
Monomorphic
11
PSS2-2
6
200
no
200
12
RM136
6
290
290
290
13
GT11
6
~200
~195
~200
14
RM229
11
~119
~119
200
116
?
15
RM457
11
~230
~232
~230
230
Polymorphic
16
RM21
11
no
no
no
157
?
17
RM473E
11
141, 139
122,119,118
118
Multiple bands
117
117,116
(repeat)
124
Polymorphic
Polymorphic
169
Polymorphic
Monomorphic
101
Monomorphic
Polymorphic
18
RM206
11
~160
~148
~120
147
? (repeat)
19
RM254
11
~156
~156
~153
165
?
20
21
PB7-8
RBO 783
Bacterial leaf blight(Xa21) 11
no
375
no
~350
no
~350
Submergence tolerance
9
? (repeat)
Monomorphic
553bp
500bp
RM121
RM21
WAXY
BADH
PSS II-1
PSS II-2
RM473E
RM206
RM254
RM21
RM229
RM457
RM50
RM121
RM136
GT11
PCR condition
1
2
3
726bp
RM597
RM190
RM204
RM205
RM225
RM111
Screening parents with SSR markers
427bp
311bp
249bp
200bp
Thermal cycling
151bp
140bp
118bp
100bp
1 KD
2 IR57514
3 KD571-77
SSR markers chosen for parents screening
# Markers
Traits
1
BADH
Aroma
8
~410
~400
2
RM190
Amylose content
6
~116
~120
3
Waxy
& Gel consistency
6
~312
~311
4
RM204
6
~119
~155
5
GT11
Gelatinization
6
~195
~200
6
PSS2-2
temperature
11
7
RM457
11
8
RM21
11
157
repeat
9
RM206
11
147
repeat
10
PB7-8 Bacterial leaf blight(Xa21) 11
Chrom IR57177 KD571-77Expected size (bp) Remark
124
169
Agarose
~232
~230
230
Agarose
2nd workshop on December
MAS for BC1F1
Planting parents
donor
KD571-77
X
early-April
recipient
late-June
IR57514
F1
mid-July
Planting F1 and recipient parents
Line selection with
selected SSR markers
early-August
F1 X IR57514
mid-October
BC1F1
mid-November
MAS
1 2 3 4……………………………….
.200 lines
late -November