Strengthening Health Systems through the Nigerian Ministry of Defense—U.S. Department of Defense Walter Reed Program Nigeria Partnership Nelson L. Michael, M.D., Ph.D Colonel, Medical Corps, U.S. Army Director US Military HIV Research Program (MHRP) Walter Reed Army Institute of Research 2012 USPHS Scientific and Training Symposium The views expressed are those of the presenter and should not be construed to represent the positions of the U.S. Army or DoD 1 20 June 2012 2 20 June 2012 Towards a Globally Effective HIV Vaccine: The role for Nigeria 3 20 June 2012 MHRP’s Product Development Plan MHRP’s vaccine development strategy emphasizes regional and global approaches. 1 BUILDING ON RV144 REGIONAL VACCINE STRATEGY Building on the RV144 outcome and lessons learned, conduct efficacy trials of the prime-boost concept in: a) Thai MSM populations b) High-risk populations in Southern Africa 2 DIVERSIFYING AND REFINING THE PORTFOLIO GLOBAL VACCINE STRATEGY Pursuing diverse platforms (e.g. vectors, multivalent constructs or mosaic inserts) that build on the prime-boost concept and readily translate to multi-clade testing and a globally effective vaccine. 4 4 20 June 2012 Pox-Protein 5 20 June 2012 NEJM 361:2209 (03 Dec 09) 6 20 June 2012 RV 144 demonstrated efficacy for HIV acquisition C. Modified Intention-to-Treat Analysis* 1.0 0.9 Placebo Probability of HIV Infection (%) 0.8 0.7 0.6 0.5 Vaccine 0.4 0.3 0.2 0.1 0.0 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 Years N=16,395 51 vaccine, 74 placebo HIV infected Est. VE = 31% 95% CI 1-51% (p=0.04) 7 Rerks-Ngarm et al. (2009, NEJM) 20 June 2012 What we have learned—RV 144 Protection among low incidence heterosexual Thais, VE 31.2% at 42 months No effect on post-infection viremia or CD4 count Relatively monophyletic circulating variants CRF01_AE Efficacy appears to be early and non-durable Evoked binding Ab but not measurable, primary isolate Nab— BAb appeared early and decreased by > 10 fold over 6 months CD4+ Env responses, but not CD8 responses Correlate/surrogate studies limited by samples and endpoints 8 20 June 2012 What we would want next Extend the observation of early 60% efficacy by increasing the durability of such protection (additional boosts) Heterosexual risk groups in Asia Ensure that we can elucidate correlates/surrogates of protection with more appropriate sample collection. Establish protection in higher incidence populations (additional boosts) 9 Heterosexuals in sub-Saharan Africa MSM in Africa and Asia 20 June 2012 NEJM 366:1275 (05 Apr 2012) 10 20 June 2012 Comparison of Infection Rate and Vaccine Efficacy Between Vaccine and Placebo Recipients in the RV144 ALVAC-HIV, AIDSVAX B/E Trial V1V2 Antibodies High V1V2 Antibodies, Increased Vaccine Efficacy Low V1V2 Antibodies, Same Infection Rate as Placebos 11 20 June 2012 Comparison of Infection Rate and Vaccine Efficacy Between Vaccinees and Placebo Recipients in the RV144 ALVAC-HIV, AIDSVAX B/E Trial IgA Magnitude and Breadth Antibodies High IgA Antibodies, No Efficacy, Same Infection Rate as Placebo—No Enhancement Low IgA, Increased Vaccine Efficacy 12 20 June 2012 Sequence variation in position 169 Edlefsen, SCHARP 13 20 June 2012 Sequence variation in position 181 Edlefsen, SCHARP 14 20 June 2012 Summary The case control correlates data suggest 2 hypotheses: Binding to gp70:V1V2 correlates inversely with HIV infection rate? • • 15 A244 and MN V2 crown linear peptides show similar effects Linear epitope microarray data suggest V2 effect Anti-Env IgA M-B correlates directly with HIV infection rate Sieve analysis suggests a V2 effect 20 June 2012 Planned studies are mutually reinforcing and will amplify public health impact and regional relevance. Precedent for vaccine efficacy RV144 Focus on regional public health impact THAILAND High Risk MSM Mutually reinforcing studies strengthen and support public health benefit in target populations and the translation of the platform globally. Future amplification of global reach US/EUROPE SOUTHEAST ASIA SOUTHERN AFRICA Republic of South Africa (RSA) High Risk Heterosexual Strategy for achieving potential licensure in target markets and having the broadest public health impact. 16 16 May 2011 20 June 2012 The pox-protein approach is regional Will we have to tailor vaccines for multiple sub-epidemics? What will be the inducement to industry to support such an approach? There are significant public health challenges with regional vaccine approaches. What about Nigeria, and the rest of West Africa, with a dominance of pure subtype G and A/G recombinant HIV infections? Can we make a universal HIV vaccine? 17 20 June 2012 Ad26-MVA +/- protein Barouch et al Nature 482:89-93 02 Feb 2012 18 20 June 2012 Nature 482, 89–93 (02 February 2012) 100 % Uninfected 80 DNA/MVA 60 MVA/MVA Ad26/MVA 40 MVA/Ad26 20 Sham 0 0 2 4 6 8 Number of IR Challenges 19 19 20 June 2012 MVA/Ad26 and Ad26/MVA Regimens Lower Early Setpoint Viral Loads Following SIVmac251 Infection 9 Sham 8 8 7 7 9 MVA/MVA 418-08 8 419-08 7 420-08 6 6 422-08 5 5 423-08 4 4 5.75 3 3 2 2 0 20 40 60 80 100 424-08 Days Following Infection 446-08 5 447-08 5.47 448-08 449-08 3 2 MEAN 20 40 60 80 100 450-08 MEAN 0 20 Days Following Infection 9 40 60 80 Ad26/MVA 426-08 3x resistance to infection 409-081 log 4/8 : viremia blunted 410-08 3/8 : rapid virologic control 411-08 1/8 : persistently uninfected 8 8 7 7 6 6 443-08 412-08 5 5 444-08 413-08 434-08 442-08 4.55 4 452-08 4 3.83 453-08 3 3 2 2 20 40 60 80 Days Following Infection 100 454-08 414-08 416-08 417-08 MEAN 0 100 Days Following Infection 9 MVA/Ad26 0 20 441-08 6 4 435-08 0 DNA/MVA 440-08 445-08 6.09 425-08 Log SIV RNA Log SIV RNA 9 MEAN 20 40 60 80 100 Days Following Infection 20 June 2012 Protection Against Acquisition of IR SIVmac251 by Ad35/Ad26-SIVsmE543GagPolEnv Vaccine 100 GagPol (N=16) GagPolEnv (N=16) % Uninfected 80 Sham (N=8) 60 40 20 0 0 2 4 6 Number of IR Challenges 21 20 June 2012 Ad26-MVA correlates analysis • • 22 Acquisition endpoint. • envelope binding antibody r= .79 p<.0001. • neutralization antibody r=.50 p=.0034 • ADCC r=.38 p=.034 set point viral load endpoint, Many correlates (N=27); • prechallenge gag elispot count and gag elispot breadth were both correlated (r=-.50 p=.006 and r=-.64 p=.0002, respectively) with the endpoint. • peak envelope binding antibody r=-.70 followed by prechallenge neutralizing antibodyr=.67. 20 June 2012 Increment 2: Pathway to a Global HIV Vaccine Phase I: Safety and immunogenicity Trials are prime-boost regimens with additional protein boost based on RV144 data Multi-clade (A/C/E) or mosaic (M1/M2) inserts Phase IIa: DNA/MVA vs Ad26/MVA (Ad35) for epitope and clade breadth and magnitude of immune response Phase IIb Efficacy#: 2011 2012 2013 2014 2015 2- or 3-arm efficacy trial with common placebo group 2016 2017 A successful outcome will yield a mosaic or multi-clade vaccine effective in high-risk populations. Commercial partners have yet to be identified and may restrict development and access to products. 23 23 . 20 June 2012 How to prepare Nigeria for HIV vaccine studies? 24 20 June 2012 Preparing Nigeria for HIV Vaccine Development Recognize a public health gap—Feb 2004 Take risk—July 2004 Recognize that you are in someone else’s country and never forget it—Jan 2005 (Bolingo) Develop durable and inclusive frameworks (steering committees, EPIC) Deliver prevention, care and treatment first (PEPFAR) 25 20 June 2012 Preparing Nigeria for HIV Vaccine Development—2 Find champions (Drs. Orits, Njoku, Idoko) Give the champions protegees—Jide, Ayemoba, Umar Build a laboratory that serves service delivery, then research (Mogadishu) Assess risk, prevalence and subtypes (RV 230) Community engagement from the inception (GPP version 2.0) 26 20 June 2012 Preparing Nigeria for HIV Vaccine Development—3 Work with partners (IHV, Harvard, Pop Council, Heartland Alliance, CDC, USAID, etc) Focus on key populations—MSM, CSW (high incidence) Engage with major stake holders (NIH, Gates) but do not vex them….let them see you take risk Advocate…UNAIDS, AVAC, AAVP South-south partnerships within MHRP 27 20 June 2012 28 20 June 2012 Acknowledgements Supported by: Collaboration for AIDS Vaccine Discovery Grant From the Bill and Melinda Gates Foundation HVTN, DAIDS, NIAID With Collaborations with the MHRP and Thai Ministry of Public Health National Institute of Allergy and Infectious Diseases (NIAID) National Institutes of Health (NIH) Division of AIDS (DAIDS) U.S. Department of Health and Human Services (HHS) Center for HIV/AIDS Vaccine Immunology (CHAVI) # U19 AI067854-06 HVTN 29 20 June 2012