A Longitudinal Study of Maternal Smoking During

A Longitudinal Study of Maternal
Smoking During Pregnancy
and Child Height
Author 1
Author 2
Author 3
Our Study
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What is the total effect of prenatal smoking on
child height from birth to adolescence?
Prospective cohort study
Longitudinal methods
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Height deficits through adolescence may lead to
increased disease risk in later life.
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 evidence for maternal anti-smoking campaigns
Summary of Literature
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Older cohort studies, some case-control
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Few longitudinal methods
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Stat. significance often not stated
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Children at birth - age 5
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Little to no height deficits after 1 year
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No evidence of interaction with alcohol
Height measurements
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Restricted to children with birth length
Recumbent height measured under age 2
Standing height measured over age 2
Most height measurements under age 2
In final analysis, only include children with height
measured at age 8 or older
Smoking Categorization
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Excluded mothers who quit during pregnancy
Self-reported
Categories
–
–
–
–
Never smoked
1-9 cigs/day
10-19 cigs/day
20+ cigs/day
Confounders
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Child age
Child sex
Child race
Child birth weight
Birth order
Gestational age
Paternal smoking during
pregnancy
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Mother’s marital status during
pregnancy
Mother’s alcohol consumption
Mother’s total number of
prenatal visits
Maternal age during pregnancy
Maternal pre-pregnancy weight
Maternal height
Maternal education
Dataset restricted to:
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Singleton births
No severe congenital abnormalities
Live births
First pregnancies only
Has maternal smoking variable
Mother did not quit smoking during pregnancy
Children age 9 and under
Birth length measured
Children with height measurements at or after age 8
See Figure 1
Characteristics of Smokers
17%
11%
54%
18%
Non smokers
1-9 cigs/day
10-19 cigs/day
20+ cigs/day
Smokers are more likely to be:
 White
 Less educated
 Drinkers
 Married to smokers
 Thinner
See Table 1
Crude Plot of Height and Age by
Smoking Level
Mean height by age and maternal smoking during pregnancy
120
100
80
60
Mean Height (cm)
140
Cigarettes/day
0
2
4
Age (yrs)
Never smoked
10-19 cigs/day
6
1-9 cigs/day
20+ cigs/day
8
Mean Height by Age and Child Race
120
100
80
60
Mean Height (cm)
140
Mean height by age and by child race
0
2
4
Age (yrs)
White
Other
6
Black/African-American
8
Mean Height by Age and Birthweight
120
100
80
60
Mean Height (cm)
140
Mean height by age and by birth weight
0
2
4
Age (yrs)
Birth weight <=88 oz
6
Birth weight >88 oz
8
Statistical Model
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Longitudinal data
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–
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Individuals’ repeat measurements are correlated
Ignoring correlation affects precision of parameter estimates
()
Generalized estimating equations (GEE)
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–
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Must specify link function, covariance structure, standard error
estimation
Covariance structure accounts for covariance due to repeated
measurements
Estimates of SE () are not affected by misspecification of the
correlation model
Our GEE model
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Link function: Identity
–
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Correlation structure: Independent
–
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Since outcome variable is continuous
No correlation between repeat measurements
Standard error estimation: Robust
Missing data
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Data was missing for many covariates
Assumed to be missing at random (MAR)
Weighting
–
–
–
Uses complete cases only
Up-weights children with covariate distributions
similar to people dropped due to missingness
Increases precision of SE estimates
Model Fitting:
Transformations of Age
Linear
 Log-linear
 Linear spline (knots between age 0.5 and 1.5)
 Quadratic spline
 Cubic spline
 Compare using graphs and quasi-likelihood
criterion (QIC)
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Model Fitting
100
100
0
50
50
Height (cm)
150
150
200
200
Crude
plot
of knot
height
age
1.5
age
at by
with
spline
Cubic
2
0
6
4
10
8
0
Age (years)
0
2 Height (cm) 4
Predicted 6height (cm)
Age (years)
8
10
Height   0  1 (age - 1.5)    2 (age - 1.5) 2  3 (age - 1.5) 3   4 X 4     k X k
Main Effects Output for Exposure
Weighted vs. Unweighted
SE
SE

(Weighted)
(Unweighted)
1-9 cigs/day
-0.152
0.17176
0.17173
-0.4883
0.1850
10-19 cigs/day
-0.590
0.23030
0.23029
-1.0415
-0.1388
20+ cigs/day
-0.163
0.20073
0.20071
-0.5564
0.2304
Weighted 95% CI
Covariates’ Effects on Height
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Increase height
–
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Decrease height
–
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African American/Black, older maternal age, taller
mothers, and higher birthweight, male sex
Older gestational age and later birth order
No statistically significant effect
–
Maternal alcohol use, education, paternal smoking
during pregnancy, marital status, maternal prepregnancy BMI and # of maternal prenatal visits
See Regression Table
Interactions Explored
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Alcohol
(2 = 3.54, df = 3, p = 0.3154 )
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Paternal smoking during pregnancy
(2 = 0.89, df = 1, p = 0.3448)
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Child age
(2 = 16.10, df = 12, p = 0.1866)
Interactions with Age
Height(cm)
(cm)
Height
145
125
Non-s m oker
105
1-9 cigs /day
10-19 cigs /day
85
20+ cigs /day
65
45
0
2
4
6
8
10
Age (years)
Test for interaction:
chi-square (df=12) = 16.10; p-value = 0.1866
Summary of Our Results
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Interaction model confirms crude smoking
trend
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Main effects model suggests dubious lack of
dose response relationship
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Growth rate does not differ between smoking
levels
Strengths
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Greater age range
Longitudinal methods
Less recall bias for sensitive subject
Limitations
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Limited external generalizability
Potential selection bias due to restriction on primary
exposure
Correlation structure assumes no relationship between
repeat height measurements
Model for weights could be misspecified
Self-reported primary exposure
Mother reported paternal smoking
No control of time-dependent confounding
Unable to explore relationship through adolescence
Insufficient number observations for caffeine
Directed Acyclic Graph (DAG):
Total Effects
Directed Acyclic Graph (DAG):
Time Dependent Confounding
Future Steps
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Explore unexpected trend across smoking
levels
Investigate direct effect of maternal smoking
during pregnancy on child height
–
–
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Use more statistically advanced methods to control
for time-dependent confounding
Measure smoking and other covariates during
childhood
Examine older ages and effect of caffeine
Thank you!

Special thanks to Brenda Eskenazi, Houston
Gilbert, Alan Hubbard, Maureen Lahiff, David
Lein, and Eric Polley
Questions or comments?