Statistics for Everyone Workshop Fall 2010 Part 6A Assessing the Relationship Between 2 Numerical Variables Using Correlation Workshop presented by Linda Henkel and Laura McSweeney of Fairfield University Funded by the Core Integration Initiative and the Center for Academic Excellence at Fairfield University Assessing the Relationship Between 2 Numerical Variables With Correlation What if the research question we want to ask is whether or how strongly two variables are related to each other, but we do not have experimental control over those variables and we rely on already existing conditions? • TV viewing and obesity • Age and severity of flu symptoms • Tire pressure and gas mileage • Amount of pressure and compression of insulation Assessing the Relationship Between 2 Variables With Correlation The nature of the research question is about the association between two variables, not about whether one causes the other Correlation describes and quantifies the systematic, linear relation between variables Correlation Causation Statistics as a Tool in Scientific Research Types of Research Questions • Descriptive (What does X look like?) • Correlational (Is there an association between X and Y? As X increases, what does Y do?) • Experimental (Do changes in X cause changes in Y?) Different statistical procedures allow us to answer the different kinds of research questions Correlation: type and strength of linear relationship between X and Y Linear Regression: making predictions of Variable Y based on knowing the value of Variable X Linear Correlation Test Statistical test for Pearson correlation coefficient (r) Used for: Analyzing the strength of the linear relationship between two numerical variables Use when: Both variables are numerical (interval or ratio) and the research question is about the type and strength of relation (not about causality) Other Correlation Coefficients There are many different types of correlation coefficients – used for different types of variables -but we focus only on Pearson r Point biserial (rPB): Use when one variable is nominal and has two levels (e.g., gender [male/female], type of car [gaspowered, hybrid]) and one variable is numerical (e.g., reaction time; miles per gallon) Spearman rank order (rS): Use for ordinal data or numerical data that are not normally distributed or linear Calculators for these are available at: http://faculty.vassar.edu/lowry/VassarStats.html The Essence of the Correlation Coefficient The correlation coefficient indicates whether or not a relationship exists (association, co-occurrence, covariation) The value of the correlation coefficient tells you about the type and strength of the relationship • Positive, negative • Strong, moderately strong, weak, no relation Different Types of Correlations Positive: As X increased, Y increased and as X decreased, Y decreased Negative: As X increased, Y decreased and as X decreased, Y increased Different Types of Correlations • No systematic relation between X and Y • High values of X are associated with both high & low values of Y; • Low values of X are associated with both high & low values of Y Different Types of Correlations Curvilinear: Not straight U: As X increased, Y decreased up to a point then increased Inverted U: As X increased, Y increased up to a point, then decreased Describing Linear Correlation Pearson correlation coefficient (r) Direction/type: Positive, Negative, or No correlation Magnitude/strength: r = 1 perfect correlation r closer to 1 strong correlation r closer to 0 weak or no correlation • r is unitless; r is NOT a proportion or percentage • It doesn’t matter which variable you call X or Y when calculating r Issues in Interpreting Correlation Coefficient Magnitude of the Effect: |r| 0 to .20 .20 to .40 .40 to .60 .60 to 1.0 Conclusion About Relationship negligible to weak weak to moderate moderate to strong strong Deposit Oxide Time thickness (s) (Angstroms) 18 1059 35 1049 52 1039 52 1026 18 1001 1263 23 etc. etc. Oxide Thickness (Angstroms) Example 1: What can we say? 1600 1400 1200 1000 800 600 400 200 0 10 20 30 Deposit Time (s) 40 50 What can we say? There is little or no correlation between oxide thickness and deposit time. In fact, r = .002. Example 2: What can we say? 70 Drug Release rate (% Released ) Surface Drug Area to Release Volume Rate 1.5 60 1.05 48 0.9 39 0.75 33 0.6 30 0.65 29 60 50 40 30 20 10 0 0 0.5 1 1.5 Surface Area To Volume (mm^2/mm^3) 2 What can we say? There was a strong, positive correlation between surface area to volume and the drug release rate. As the surface area to volume increased, the drug release rate increased The smaller the surface area to volume, the lower the drug release rate What More Does the Correlation Coefficient Tell Us? 70 Drug Release Rate (%released) Surface Area to Drug Volume Release Ratio Rate 1.5 60 1.05 48 0.9 39 0.75 33 0.6 30 0.65 29 60 50 40 30 20 10 0 0 0.5 1 1.5 Surface Area To Volume Ratio (mm^2/mm^3) r = .99 2 Interpreting Correlation Coefficient A strong linear association was found between the surface area to volume and the drug release rate, r = .99 There was a significant positive correlation between the surface area to volume and the drug release rate, r = .99 As the surface area to volume increased the drug release rate increased, and this was a strong linear relationship, r = .99 Testing for Linear Correlation Correlations are descriptive We can describe the type and strength of the linear relationship between two variables by examining the r value r values are associated with p values that reflect the probability that the obtained relationship is real or is more likely just due to chance Running and Interpreting a Correlation The key research question in a correlational design is: Is there a real linear relationship between the two variables or is the obtained pattern no different what we would expect just by chance? i.e. H0: The population correlation coefficient is zero HA: The population correlation coefficient is not zero Teaching tip: In order to understand what we mean by “a real relationship,” students must understand probability What Do We Mean by “Just Due to Chance”? p value = probability of results being due to chance When the p value is high (p > .05), the obtained relationship is probably due to chance .99 .75 .55 .25 .15 .10 .07 When the p value is low (p < .05), the obtained relationship is probably NOT due to chance and more likely reflects a real relationship .04 .03 .02 .01 .001 What Do We Mean by “Just Due to Chance”? p value = probability of results being due to chance [Probability of observing your data (or more severe) if H0 were true] When the p value is high (p > .05), the obtained relationship is probably due to chance [Data likely if H0 were true] .99 .75 .55 .25 .15 .10 .07 When the p value is low (p < .05), the obtained relationship is probably NOT due to chance and more likely reflects a real relationship [Data unlikely if H0 were true, so data support HA] .04 .03 .02 .01 .001 What Do We Mean by “Just Due to Chance”? In science, a p value of .05 is a conventionally accepted cutoff point for saying when a result is more likely due to chance or more likely due to a real effect Not significant = the obtained relationship is probably due to chance; the relationship observed does not appear to really differ from what would be expected based on chance; p > .05 Statistically significant = the obtained relationship is probably NOT due to chance and is likely a real linear relationship between the two variables; p < .05 Finding the Linear Correlation Using Excel Step 1: Make a scatterplot of the data If there is a linear trend, go to Step 2 Step 2: Get the Pearson Correlation Coefficient (r) Using the = correl(X, Y) function [Refer to handouts for more detail] Running a Test for Linear Correlation Using Excel Pearson’s r: Both variables are numerical (interval or ratio) and the research question is about the type and strength of relation (not about causality) Need: • Pearson correlation coefficient (r) • Number of pairs of observations (N) To run: Open Excel file “SFE Statistical Tests” and go to page called Linear Correlation Test Enter the correlation coefficient (r) and number of pairs of observations (N) Output: Computer calculates the p value Running a Test for Linear Correlation Using SPSS When to Calculate Pearson’s r: Both variables are numerical (interval or ratio) and the research question is about the type and strength of relation (not about causality) To run: Analyze Correlate Bivariate Move the X and Y variables over. Check “Pearson Correlation” and “Two-tailed test of significance”. Then click Ok. Output: Computer calculates r and the p value Reporting Results of Correlation If the relationship was significant (p < .05) (a) Say: A [say something about size: weak, moderate, strong] [say direction: positive/negative] correlation was found between [X] and [Y] that was statistically significant, r = .xx, p = .xx (b) Describe the relation: Thus as X increased, Y [increased/decreased] Note: If the r value is positive, then as X increased, Y increased; if r is negative, then as X increased, Y decreased e.g., A strong positive correlation was found between surface area to volume ratio and the drug release rate that was statistically significant, r = .99, p = .0001. As the surface area to volume ratio increased, the drug release rate increased. Reporting Results of Correlation If the relationship was not significant (p > .05) Say: No statistically significant correlation between [X] and [Y] was found, r = .xx, p = .xx. Thus as X increased, Y neither increased nor decreased systematically. e.g., No statistically significant correlation between oxide thickness and deposit time was found, r = .002, p = .99. Thus as oxide thickness increased, deposit time neither increased nor decreased systematically. Teaching Tip Impress upon your students that an association does not imply causation e.g., Average life expectancy and the average number of TVs per household are highly correlated. But you can’t increase life expectancy by increasing the number of TVs. They are related, but it isn’t a cause-and-effect relationship. More Teaching Tips You can ask your students to report either: • the exact p value (p = .03, p = .45) • the cutoff: say either p < .05 (significant) or p > .05 (not significant) You should specify which style you expect. Ambiguity confuses them! Tell students they can only use the word “significant” only when they mean it (i.e., the probability the results are due to chance is less than 5%) and to not use it with adjectives (i.e., they often mistakenly think one test can be “more significant” or “less significant” than another). Emphasize that “significant” is a cutoff that is either met or not met -- Just like you are either found guilty or not guilty, pregnant or not pregnant. There are no gradients. Lower p values = less likelihood results are due to chance, not “more significant” Correlations are Predictive You can predict one variable from the other if there is a strong correlation. If so, linear regression can be used to find a linear model which can be used to make predictions. But, remember to make a scatterplot of the data to see if a linear model is appropriate and/or the best model! Correlation: type and strength of linear relationship between X and Y Linear Regression: making predictions of Variable Y based on knowing the value of Variable X Example 1 Hours of protection vs. Cost 25 Hours of Protection 20 15 10 5 0 0 0.5 1 1.5 2 Cost per ounce ($) A linear regression seems appropriate since the data have an overall linear trend. 2.5 Example 2 Mass of spill (lbs). Mass of chemical spill at time t r = -.92 7 6 5 4 3 2 1 0 0 10 20 30 40 50 60 70 Time (minutes) Even though r = -.92 a linear regression model is not appropriate here. A different model should be used. Terminology X variable: predictor (independent variable, explanatory variable, what we know) Y variable: criterion (dependent variable, response variable, what we want to predict) Stronger correlation = better prediction Making Predictions 1600 1400 Oxide thickness Oxide thickness Time (s) (Angstroms) 18 1059 35 1049 52 1039 52 1026 18 1001 1263 23 etc. etc. 1200 1000 800 600 400 200 0 10 20 30 Time 40 50 Making Predictions 70 Drug Release Rate (%released) Surface Area to Drug Volume Release Ratio Rate 1.5 60 1.05 48 0.9 39 0.75 33 0.6 30 0.65 29 60 50 40 30 20 10 0 0 0.5 1 1.5 Surface Area To Volume Ratio (mm^2/mm^3) 2 Using Regression to Make Predictions Low or no correlation (e.g., Oxide thickness and Deposit time) • Best prediction of Y is the mean of Y (knowing X doesn’t add anything) High correlation (e.g., Surface area to volume ratio vs. Drug release rate ) • Best prediction of Y is based on knowing X Sample Scatterplots & Regression Lines If r = +1, Y’ = Y If r = 0, Y’ = MY Terminology Regression line: • Best fitting straight line that summarizes a linear relation • Comprised of the predicted values of Y (denoted by Y’ ) What Do We Mean by the “Best Fit” Line? Comparing Amounts of Snowfall Gauge Measured (inches of snow) 10 8 6 4 2 0 0 2 4 6 Radar Prediction (inches of snow) 8 What Do We Mean by the “Best Fit” Line? Comparing Amounts of Snowfall Gauge Measured (inches of snow) 10 8 6 4 2 0 0 2 4 6 Radar Prediction (inches of snow) 8 What Do We Mean by the “Best Fit” Line? It is the line that minimizes the vertical distances from the observed points to the line Comparing Amounts of Snowfall Gauge Measured (inches of snow) 10 8 6 4 2 0 0 2 4 6 Radar Prediction (inches of snow) 8 Least Squares Line Error or residual = observed Y – predicted Y = Y – Y’ The least-squares line is the “best fit” line that minimizes the sum of the square errors/residuals i.e: It minimizes Y Y' 2 Best Fit Line Also known as linear regression model or least squares line Used to predict Y using a single predictor X and a linear model Y’ = bX + a Y’ = predicted y-value X = known x-value b = slope a = y-intercept or the point where line crosses y-axis [We can use more advanced techniques for multiple predictors or nonlinear models] Interpreting the Model Y’ = bX + a What is b? • Slope of the regression line • The ratio of how much Y changes relative to a change in one unit of X • Same sign as the correlation What is a? • Y-intercept or where the line crosses the Y axis (where X = 0) Getting the Least Squares Line While Excel will get the least squares line, we give the following formulas for completeness. b r SD Y SD X a M Y b( M X ) Getting the Least Squares Line in Excel Linear Regression: When you want to predict a numerical variable Y from another numerical variable X Need: • Enter X and Y data in Excel • Be sure that X column is directly to the left of the Y column To get linear model: Make a scatterplot of Y vs. X. Click on the Chart and choose Chart/Add Trendline Choose the Option tab and select Display equation Output: Least squares line Getting the Least Squares Line in SPSS Linear Regression: When you want to predict a numerical variable Y from another numerical variable X Need: Enter X and Y data in SPSS To get linear model: Analyze Regression Linear; Move the Y variable to Dependent and the X variable to Independent; Click Ok. Output: Least squares line Example X = Surface Area to Volume Ratio (mm2/mm3) Y = Drug Release Rate (%released) The least squares line is Y’ = 36.92X + 7.21 If surface area to volume ratio is 1, what is predicted drug release rate? ______ If the actual drug release rate was 40%, what is the residual? ________ Example X = Surface Area to Volume Ratio (mm2/mm3) Y = Drug Release Rate (%released) The least squares line is Y’ = 36.92X + 7.21 If surface area to volume ratio is 1, what is predicted drug release rate? Y’ = 36.92(1)+7.21 = 44.13% released If the actual drug release rate was 40%, what is the residual? Residual = Y – Y’ = 40 – 44.13 = -4.13% Example X = Surface Area to Volume Ratio (mm2/mm3) Y = Drug Release Rate (%released) The least squares line is Y’ = 36.92X + 7.21 By how much can you expect the drug release rate to increase or decrease if you increase the surface area to volume ratio by 1 mm2/mm3?_________ Example X = Surface Area to Volume Ratio (mm2/mm3) Y = Drug Release Rate (%released) The least squares line is Y’ = 36.92X + 7.21 By how much can you expect the drug release rate to increase or decrease if you increase the surface area to volume ratio by 1 mm2/mm3? This is just the slope, so you would expect the drug release rate to increase by 36.92% What about R2? Often in correlational research the value R2 is reported. R2 = Proportion of variability of Y accounted for by X and the linear model For example, if R2 = .64 then we can say: • 64% of the variance in the Y scores can be predicted from Y’s (linear) relation with X • Predictions are 64% more accurate using the linear regression equation to make predictions (Y’) than when we use MY to make predictions How do you get R2? R2 is literally the correlation coefficient, r, times itself. R2 = (r)2 0 R2 1 Low r low R2 little improvement over MY High r high R2 more accurate predictions using Y’ How do you get r from R2? r = Sqrt(R2) or r = – Sqrt(R2) Remember, the correlation (r) has the same sign as the slope of the least squares line. Issues to Consider 1. You can’t extrapolate outside the given domain of the X’s 2. Be careful of the effect of potential outliers 3. Remember to impress upon your students that an association does not imply causation, no matter how seductive it may be to think that the 2 variables are causally related You can’t extrapolate outside the given domain of the X’s You can only make predictions in the range of the given data. We don’t know if the pattern we see continues for surface area to volume ratios less than .6 and greater than 1.5 mm2/mm3. Drug Release Rate (%released) Example y = 35.916x + 7.2094 70 60 50 40 30 20 10 0 0 0.5 1 1.5 Surface Area To Volume Ratio (mm^2/mm^3) 2 Be careful of the effect of potential outliers Outliers can be influential or not. Exploring Linear Regression Excel Worksheet Hypothesis Testing For Simple Linear Regression Y = + X+ where is normally distributed with mean 0 and variance 2 The F test allows a scientist to determine whether their research hypothesis is supported Null hypothesis H0: • There is not a linear relationship between X and Y • There is no correlation between X and Y • =0 Hypothesis Testing For Simple Linear Regression Research hypothesis HA: • X is linearly related to Y • The correlation between X and Y is different from 0 • 0 Teaching tip: Very important for students to be able to understand and state the research question so that they see that statistics is a tool to answer that question Hypothesis Testing For Simple Linear Regression Null hypothesis: Pain level is not linearly related to weight Research hypothesis: Pain level is linearly related to weight Null hypothesis: There is no linear relationship between age and heart rate Research hypothesis: There is a linear relationship between age and heart rate Hypothesis Testing For Simple Linear Regression p value = probability of results being due to chance When the p value is high (p > .05), the obtained relationship is probably due to chance .99 .75 .55 .25 .15 .10 .07 When the p value is low (p < .05), the obtained relationship is probably NOT due to chance and more likely reflects a real linear relationship between X and Y .04 .03 .02 .01 .001 Hypothesis Testing For Simple Linear Regression In science, a p value of .05 is a conventionally accepted cutoff point for saying when a result is more likely due to chance or more likely due to a real effect Not significant = the obtained relationship is probably due to chance; X does not appear to be linearly related to Y; p > .05 Statistically significant = the obtained relationship is probably NOT due to chance and X and Y appear to be linearly related; p < .05 Sources of Variance in Simple Linear Regression SSTotal = SSRegression + SSResidual (Yi – MY)2 = (Yi’ – MY)2 + (Yi – Yi’)2 How much do all the individual scores differ from the grand mean How much do all the predicted values differ from the grand mean How much do the individual scores differ from the predicted values Total Sums of Squares Regression Sums Residual Sums of Squares of Squares Each F test has certain values for degrees of freedom (df), which is based on the sample size (N) and number of conditions, and the F value will be associated with a particular p value SPSS calculates these numbers. Summary Table for Simple Linear Regression Source Sum of Squares (SS) df Mean Square (MS) Regression (Yi’ – MY)2 1 SSRegression dfRegression Residual (Error) (Yi – Yi’)2 N-2 SSResidual dfResidual Total (Yi – MY)2 N-1 F MSRegression MSResidual N= # samples To report, use the format: F(dfRegression, dfResidual) = x.xx, p _____. The F Ratio A test for simple linear regression gives you an F ratio The bigger the F value, the less likely the relationship between X and Y is just due to chance The bigger the F value, the more likely the relationship between X and Y is not just due to chance and is due to a real relationship So big values of F will be associated with small p values that indicate the linear relationship is significant (p < .05) Little values of F (i.e., close to 1) will be associated with larger p values that indicate the linear relationship is not significant (p > .05) Interpreting the F test for Simple Linear Regression Cardinal rule: Scientists do not say “prove”! Conclusions are based on probability (likely due to chance, likely a real effect…). Be explicit about this to your students. Based on p value, determine whether you have evidence to conclude the relationship was probably real or was probably due to chance: Is the research hypothesis supported? p < .05: Significant • Reject null hypothesis and support research hypothesis (the relationship was probably real; X is linearly related to Y) p > .05: Not significant • Retain null hypothesis and reject research hypothesis (any relationship was probably due to chance; X is not linearly related to Y) Teaching Tips Students have trouble understanding what is less than .05 and what is greater, so a little redundancy will go a long way! Whenever you say “p is less than point oh-five” also say, “so the probability that relationship is due to chance is less than 5%, so the 2 variables are likely to be linearly related.” Whenever you say “p is greater than point oh-five” also say, “so the probability that this is due to chance is greater than 5%, so there’s just not enough evidence to conclude that there is a linear relationship – X and Y are not really linearly related” In other words, read the p value as a percentage, as odds, “the odds that this relationship is due to chance are 1%, so X and Y probably are linearly related…” Running the F Test for Simple Linear Regression in SPSS Linear Regression: When you want to test whether there is a linear relationship between two numerical variables (X and Y) Need: Enter X and Y data in SPSS To get linear model: Analyze Regression Linear; Move the Y variable to Dependent and the X variable to Independent; Click Ok. Output: F and p-value Reporting the Results of the F Test for Simple Linear Regression Step 1: Write a sentence that clearly indicates what statistical analysis you used An F test was used to determine if there was a linear relationship between [X] and [Y]. -Or - An F test was used to determine if [X] is linearly related to [Y]. An F test was used to determine if pain level is linearly related to weight. An F test was used to determine if there is a linear relationship between age and heart rate. Reporting the Results of the F Test for Simple Linear Regression Step 2: Report whether the linear relationship was significant or not The linear relationship between [X] and [Y] was significant [or not significant], F(dfRegression, dfResidual) = X.XX [fill in F], p = xxxx. The linear relationship between pain level and weight was significant, F(2, 40) = 7.31, p = .01. The linear relationship between age and heart rate was not significant, F(2, 120) = 2.35, p = .10. Assumptions Involved in Simple Linear Regression • Linearity • Randomness and Normality • Homoscedasticity Assessing Linearity Is a linear model appropriate? Is a linear model the best model? To check this make a scatterplot of the data and see if there is an overall linear trend. Assessing Linearity Hours of protection vs. Cost 25 Hours of Protection 20 15 10 5 0 0 0.5 1 1.5 Cost per ounce ($) Linear model seems appropriate. 2 2.5 Assessing Linearity Mass of spill (lbs). Mass of chemical spill at time t 7 6 5 4 3 2 1 0 0 10 20 30 40 50 60 Time (minutes) Linear regression is not appropriate here. Should use a different model. 70 Assessing Randomness and Normality of Residuals Are the residuals random and normally distributed? The data should be randomly scattered about the least squares line. There should be no patterns about the least squares line. Assessing Normality of Residuals Hours of protection vs. Cost 25 Hours of Protection 20 15 10 5 0 0 0.5 1 1.5 2 2.5 Cost per ounce ($) Points are randomly scattered about least squares line. Assessing Normality of Residuals Mass of chemical spill at time t Mass of spill (lbs). 8 6 4 2 0 -2 0 10 20 30 40 50 60 70 -4 Time (minutes) Points are not randomly scattered about least squares line, so the residuals are not random. Assessing Homoscedasticity Homoscedasticity = the variability or scatter of points about the best fit line is fairly constant Check if line fits consistently well for all X’s. Assessing Homoscedasticity Hours of protection vs. Cost 25 Hours of Protection 20 15 10 5 0 0 0.5 1 1.5 2 2.5 Cost per ounce ($) Line fits consistently well throughout. Since all three assumptions are met for this data set, a linear model is appropriate. Assessing Homoscedasticity Height vs. Weight 80 Height (in) 75 70 65 60 55 50 100 120 140 160 180 200 220 240 Weight (lbs) The linear model fits better for weights more than 140. So, there may be a problem with homoscedasticity. Time to Practice Correlation and Linear Regression