Teresa Audesirk • Gerald Audesirk • Bruce E. Byers Biology: Life on Earth Eighth Edition Chapter 7 Capturing Solar Energy: Photosynthesis Copyright © 2008 Pearson Prentice Hall, Inc. Chapter 7 Opener Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (chloroplast) FOTOSINTESIS H2O CO2 ATP RESPIRACION CELULAR (mitochondrion) Figure 7-1 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. GLUCOSA O2 (a) Leaves (b) Internal leaf structure cuticle upper epidermis mesophyll cells (d) Chloroplast lower epidermis chloroplasts stoma outer membrane inner membrane thylakoid vascular bundle (vein) stoma bundle sheath stroma channel interconnecting thylakoids Figure 7-2 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (c) Mesophyll cell containing chloroplasts Leaves Figure 7-2a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Internal leaf structure cuticle upper epidermis mesophyll cells lower epidermis stoma stoma vascular bundle bundle sheath (vein) Figure 7-2b Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. chloroplasts Mesophyll cell containing chloroplasts Figure 7-2c Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Chloroplast outer membrane inner membrane thylakoid stroma channel interconnecting thylakoids Figure 7-2d Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Figure 7-3 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. H 2O LIGHT-DEPENDENT REACTIONS (in thylakoids) DEPLETED CARRIERS (ADP, NADP+) CO2 ENERGIZED CARRIERS (ATP, NADPH) LIGHT-INDEPENDENT REACTIONS (in stroma) Figure 7-4 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. O2 glucose Absorbance of photosynthetic pigments light absorption (percent) chlorophyll b carotenoids chlorophyll a Wavelength (nanometers) Visible light Gamma rays X-rays UV higher energy (too much) Infrared Microwaves Radio waves lower energy (not enough) Figure 7-5 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Figure 7-6 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. energy level of electrons sunlight 7 6 3 8 2e– 5 2e– 2 2e– 4 energy to drive ATP synthesis reaction center 1 HO9 2e– 2 2H+ photosystem II 1/2 O2 Figure 7-7 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. photosystem I 2e– NADPH NADP+ H+ thylakoid chloroplast Figure 7-8 (part 1) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Energy from energized electrons powers active transport of H+ by ETC. PSII ETC PSI Energy-carrier molecules power the C3 cycle. stroma ETC C3 cycle Energy from energized electrons powers NADPH synthesis. thylakoid space High H+ concentration generated by active transport. H+ channel coupled to ATP-synthesizing enzyme. Figure 7-8 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Flow of H+ down concentration gradient powers ATP synthesis. 1 Energy is released as water flows downhill. 2 Energy is harnessed to rotate turbine. 3 Energy of rotating turbine is used to generate electricity. Figure E7-1 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. thylakoid chloroplast Figure E7-2 (part 1) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. photosystem II Active transport of hydrogen ions. thylakoid membrane 2e– stroma High H+ concentration in thylakoid space. H+ ion channel coupled to ATP synthesizing enzyme. Flow of H+ powers ATP synthesis. ADP P ATP Figure E7-2 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Figure 7-9 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 1 Carbon fixation combines CO2 with RuBP. 6 CO2 2 G3P synthesis uses energy. 6 12 6 RuBP 3 RuBP synthesis uses energy and 10 G3P. PGA C3 cycle (Calvin-Benson cycle) 12 ATP 12 ADP 6 ADP 6 ATP 4 G3P available for synthesis of glucose. 12 NADPH 12 G3P glucose (or other molecules) Figure 7-10 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 12 NADP+ energy from sunlight O2 CO2 ATP NADPH Light-dependent reactions are associated with thylakoids. ADP Lightindependent reactions (C3 cycle) occur in stroma. NADP+ H2O chloroplast sugar Figure 7-11 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. C3 plants use the C3 pathway Much photorespiration occurs under hot, dry conditions. CO2 O2 PGA C3 Cycle rubisco CO2 RuBP G3P glucose stoma within mesophyll chloropast bundlesheath Little glucose cells is synthesized. In a C3 plant, mesophyll cells contain chloroplasts; bundlesheath cells do not. Figure 7-12a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. C4 plants use the C4 pathway CO2 is captured with a highly specific enzyme. CO2 PEP AMP C4 Pathway 4-carbon molecule ATP pyruvate PGA stoma bundlesheath cells within mesophyll chloropast CO2 O2 rubisco C3 Cycle G3P glucose In a C4 plant, both mesophyll and bundle-sheath cells contain chloroplasts. CO2 RuBP Almost no photorespiration occurs in hot, dry conditions. within bundle-sheath chloropast Lots of glucose is synthesized. Figure 7-12b Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.