The innovative mould inhibitor
for guaranteed results even
in extreme conditions
Name of the European Project:
“REAL TIME METHODOLOGIES FOR THE
PREDICTION AND CONTROL OF FUNGAL
CONTAMINATIONS, IN FEED STUFFS”
• Complutense University Of Madrid (Spain)- Faculty of
Veterinary Medicine – Dept. Of Toxicology – prof. A.
Anadon
• Catholic University of Piacenza (Italy) – Faculty of
Agronomics – Dept. Of Animal Nutrition – Prof.
G.Piva
• Institute Nacional Reseaches Appliqueés (INRA) de
Tulose (France) – Dept.of Moulds & Fungi – prof.J.Le
Bars
• Dox-al Italia spa – Italy
• Dox-al Iberica SA – Spain
• Dox-al France SA- France
N. 2 MINISILOS
Probes Positioning
Silo “A”
Silo “B”
1- probe 0-1 (regolabile)
6- probe 10-11
(adjustable)
2- probe 2-3 (south side)
7- probe 12-13 (south
side)
3 probe 4-5 (north side)
8 probe14-15 (north
side)
4 probe 6-7
9 probe 16-17
5 probe 8-9
10 probe 18-19
External probes 21 (A)/26(B) north side
External probes 23(A)/27(B) south side
External Ambient temperature probe
Sampling point (product/CO2)
Probe entrance point
X-ray irradiation
Proyect Ref. AIR 3-CT94-1325
Partecipant :
UNIVERSIDAD COMPLUTENSE DE MADRID
FACULTAD DE VETERINARIA CATEDRA DE
TOXICOLOGIA Y LEGISLACION SANITARIA
Resercher in charge: Prof.Dr. Arturo Anadòn
SUMMARY OF TECHNICAL REPORTS
 Task C 6.1: Toxicity testing: Studies in vivo/in vitro with
hepatocytes for cytotoxicity detection of several mycostatic
agents and/or mycotoxins
 Task C 6.1.1.: Toxicity testing: Studies in vitro with hepatocytes
for cytotoxicity detection of a potential mycostatic agent, 8quinolinol sulfate
 Task C 6.2: Toxicity testing: In vivo and in vitgro assessment of
enzymatic induction in experimental animals
 Task C 6.2.1: Toxicity testing Effect of the mycotoxin Fumonisin
B1 on rat hepatic microsomal cytochrome P450 system and
peroxisomal enzymes
 Task C 6.2.2: Toxicity testing: effect of the mycostatic agents
chloramine-T (PE3-3) and natamycin on rat hepatic microsomal
cytochrome P450 system and perozisomal enzymes.
Task C 6.1.1: Toxicity testing: Studies in vitro with hepatocytes for
cytotoxicity detection of a potential mycostatic agent, 8-quinolinol sulfate
1.
2.
Introduction
Material and methods
2.1 Animal protocol and experimental
desigh
2.2 Cytotoxicity assays
2.2.1 Lactate dehydrogenase release
2.2.2 MTT reduction assay
2.2.3 Glutathione assay
2.3. Data analysis
3. Results and conclusions
Task C 6.2: Toxicity testing: In vivo and in vitgro assessment of
enzymatic induction in experimental animals
Task C 6.2.1: Toxicity testing Effect of the mycotoxin Fumonisin B1 on rat
hepatic microsomal cytochrome P450 system and peroxisomal enzymes
1. Introduction
2. Material and methods
2.1 Treatment of animals
2.2 Enzyme assays and biochemical
determinations
3.
Results and conclusions
Task C 6.2.2: Toxicity testing: effect of the
mycostatic agents chloramine-T (PE3-3) and
natamycin on rat hepatic microsomal cytochrome
P450 system and perozisomal enzymes.
1. Introduction
2. Material and methods
2.1 Treatment of animals
2.2 Enzyme assays and biochemical
determinations
3. Results and conclusions
the identification of
Aspergillus glaucus
as the harmless “pioneer”,
which increased level can be used as
an alarming signal
the ergosterole presence
as a marker for a rapid quantitative
determination of moulds
ERGOSTEROLE CONTENT OF MAIN FEED INGREDIENTS
Name
Average
content
(mg/kg
Good
quality
(mg/kg)
Risky
quality(
mg/kg)
Name
Average
content
(mg/kg
Good
quality
(mg/kg)
Risky
quality(m
g/kg)
Corn
3.9
<3
>8
Peas
1.1
<2
>4
Sorghum
5.8
<8
>12
Rapeseed
9.5
<15
>20
Wheat
5.9
<8
>12
Manioca
28.3
<30
>50
Barley
8.4
<9
>14
Soyabean meal
1.4
<3
>4
Rye
6.6
<8
>12
Triticale
7.3
<8
>12
Peanut meal
3.4
<6
>9
Wheat meal
4.2
<5
>10
Sunflower meal 3.8
<6
>9
Wheat bran
9.9
<10
>15
Copra
12.1
<15
>20
Corn meal
4.0
>8
Cotton seed
31.7
<35
>50
Corn feed
24.4
<25
>35
Citrus pulp
3.0
<5
>8
Corn gluten
feed
40.8
<50
>70
Molasses
2.9
<4
Corn distiller
46.0
<50
>70
Dried alfalfa
35.0
<50
Corn solubles
36.4
<50
>70
Straw granules
124.8
<130
Lupins
0.5
<2
>4
>80
a clearer spectrum of activity
of each traditional organic acid
the identification of the role of
• sulphur
• ammonium
• chlorine
the discovery of new molecules
able to actively
stop mould proliferation
A FEW FACTORS
INFLUENCING
FEED PRESERVATION TIME
ASPECT:
pellet process
increases the preservation time
2 folds
compared to mash form
TEMPERATURE:
an increase of 5°C in storage
decreases the preservation time
2 folds
WATER CONTENT:
an increase of 0.5%
of water content
decreases the preservation time
2 folds
MOULD CONTAMINATION:
an increase of
2 Log of mould population
decreases the preservation time
2 folds
TEMPERATURE VARIATIONS:
a delta of more than
10°C inside a silo,
triggers water migration
PROTECTION FACTOR (PF)
is an index indicating
the ability of a substance to prolong
feed shelf life
PROTECTION FACTORS OF SOME SUBSTANCES:
PROPIONIC ACID
SODIUM PROPIONATE
SORBIC ACID
POTASSIUM SORBATE
CITRIC ACID
BENZOIC ACID
2.15
2.02
1.38
1.41
0.97
1.00
PROTECTION FACTOR
CERQUAL: 3
PROTECTION FACTOR
Cerqual PF is
higher than the PF of each one
of its ingredients:
SYNERGY
COMPOSITION
AMMONIUM ACETATE • SULPHUR • CHLORINE
ACETIC ACID • PROPIONIC ACID
HYDROXY Ca – Al – Mg SILICATE
Cerqual doesn’t contain any excipient
DOSAGE
Cerqual dose depends on:
• STORAGE TEMPERATURE
• STORAGE LENGTH
• MOISTURE CONTENT
To tailor Cerqual dosage to your own
needings, ask for the
SMART CHART
SMART CHART