Study of Influence of Salts Additives to
Mixed Reverse Micelles via Reverse
Microemulsion Chromatography and UVvisible spectroscopy
Manuchar Gvaramia,
Postgraduate student, Department of Chemistry,
Faculty of Exact and Natural Sciences,
Tbilisi University, Georgia
Reverse micelle
Direct micelle
Mixed microemulsions on the basis of
nonionic and anionic surfactants
Caff
8
PB
CBZ
7
Retention factor, k
HQ
6
5
4
3
2
1
0
0
1
2
3
4
5
6
Water/surfactant ratio
Dependence of retention factors of solutes on the
water/surfactant ratio
6
RC
PL
NB
Retention factor, k
5
4
3
2
1
0
0
1
2
3
4
5
6
Water/surfactant ratio
Dependence of retention factors of solutes on the
water/surfactant ratio
Water,Brij-30, butanol and hexane with ratio
(v/v.%): 0.3/2.8/4.6/92.3
Water,Brij-30, butanol and hexane with ratio
(v/v.%): 0.7/2.8/4.6/91.9
w=2
8
w=3
Retention factor, k
6
4
2
0
HQ
PB
CBZ
Caff
Diagram of retention factors of solutes at w=2( ) and w=3 ( ) in the presence of
sodium acetate(0.025M)
w=2
8
w=3
Retention factor, k
6
4
2
0
NB
RC
PL
Diagram of retention factors of solutes at w=2( ) and w=3 ( ) in the presence of
sodium acetate(0.025M)
w=2
8
w=3
Retention factor, k
6
4
2
0
HQ
PB
CBZ
Caff
Diagram of retention factors of solutes at w=2() and w=3() in the presence of
potassium perchlotate(0.025)
8
w=2
w=3
Retention factor, k
6
4
2
0
NB
RC
PL
Diagram of retention factors of solutes at w=2() and w=3() in the presence of
potassium perchlotate(0.025)
8
Retention factor, k
6
4
2
0
RC
PL
CBZ
Caff
PB
Diagram of retention factors of solutes at the presense of sodium acetate () and
potassium perchlorate() W=2
O-nitroaniline as molecular
probe
Determination of binding constants of onitroaniline (o-NA) to Brij-30 reverse micelles
[o  NA]b
Kb 
[o  NA] f [ Brij  30] 0

  o  NAf    o  NAb  A376
 398
398






o

NA



o

NA

f
b  A398
f
b

376
f
376
b
0,18
0,16
0,14
Absorbance
0,12
0
0,1
0.13
0.91
0,08
2.73
11.7
0,06
23.4
0,04
0,02
0
300
350
400
450
500
Wavelength/nm
Absorption spectra of o-NA in hexane/(♦-0.0M Brij-30+ 0.1M AOT, 0.13 ·10-2M
Brij-30+ 0.1AOT, 0.91·10-2M Brij-30+ 0.1M AOT, 2.73·10-2M Brij-30+ 0.1M AOT,
11.7·10-2M Brij-30+0.1M AOT, 23.4·10-2M Brij-30+ 0.1M AOT) at Brij-30
concentrations. At W0=0, [o-NA]=4.5·10-5M
Variation of λm of o-NA in reverse micelles as a
function of Brij-30 concentration
Diagram of Kb of o-NA with (Brij-30+AOT) mixed micelles in
the following conditions:1) absence of water, 2) presence of
water, 3) presence of 0.05M sodium acetate and 4) 0.05M
potassium perchlorate
b
a
8
100
Kb, M -1
Retention factor, k
6
4
50
2
0
RC
PL
CBZ
Caff
PB
0
a) Diagram of retention factors of solutes in the presence of sodium acetate (■)
and potassium perchlorate (□) W=2
b) Diagram of binding constants of o-NA with (Brij-30+AOT) mixed micelles in
the presence of 0.05M sodium acetate(■) and 0.05M potassium perchlorate(□)
Conclusions
• Binding of o-nitroaniline to reverse micelles is
higher in the presence of chaotropic perchlorate
ions in comparison with kosmotropic acetate
ions.
• Solubility of the model compounds is lower in the
presence of chaotropic anions in the water pools
of reverse micelles as compared with
kosmotropic acetate ions.
• Retention factor of the solutes are higher in the
presence of kosmotropic acetate ions in the
water nanocages of the reverse micelles as
compared to perchlorate ions.
Conclusions
• Results may be useful in the investigations
of water structure, when it is confined to
nanometer-scale cavities, viz. in biological
systems.
Acknowlegement
• I thank the Georgian Shota Rustaveli
National Science Foundation and Julich
Scientific Center for opportunity to
participate in this project
Thanks for attention
•
2SO4 > F > OAc >
ClO4 > SCN
-
-
Cl > Br >
NO3
-
>I >
Benzene-1,3-diol
resorcinol
benzene-1,4-diol
hydroquinone
benzene-1,2,3-triol
pyrogallol
1,3,7-trimethyl-1H-purine-2,6(3H,7H)-dione
caffeine
1,3-dimethyl-7H-purine-2,6-dione
theophylline
2,2,2-trichloroethane-1,1-diol
Phenobarbital (5-ethyl-5-phenylbarbituric acid or
5-ethyl-5-phenylhexahydropyrimindin-2,4,6-trione)
carbamazepine
Absorption spectra of o-NA in different solvents:1)
water (412), 2) ethanol (406 nm), 3) methanol
(405nm) and 4)isopropanol (407 nm)
Possible scheme of arrangement of
ions in water pools of the reverse
micelle