Programme PhD / Master course
Advanced Microscopy and Vital Imaging
2015
Monday, June 8, 2015
Time
09.00 – 09.15
Item
Welcome and brief overview of the course
09.15 – 10.00
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
Basics of Microscopy
10.15 – 12.45
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
Practical course: Bright field microscopy, a hands-on course
GROUP I
Location
UNS 40
A.0.771
UNS 40
A.0.771
UNS 40
C.4.577
Jos Broers/Helma Kuijpers
Maastricht University Medical Center
10.15 – 11.15
Fluorescence microscopy
GROUP II
UNS 40
A.0.771
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
13.30 – 14.30
Fluorescence microscopy
GROUP I
UNS 40
A.0.771
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
13.30 – 16.00
Practical course: Bright field microscopy, a hands-on course
GROUP II
Jos Broers/Helma Kuijpers
Maastricht University Medical Center
NVvM
UNS 40
C.4.577
Tuesday, June 9, 2015
Time
09.00 – 10.00
Item
Light sheet microscopy (SPIM)
10.00 – 11.00
Rudolf Leube
Uniklinik RWTH Aachen – Institut für Molekulare und Zelluläre
Anatomie
Live cell imaging using GFP-technology in combination with CSLM
11.00 – 12.00
14.00 – 15.00
11.00 – 14.00
Jos Broers
Dept. Molecular Cell Biology, Maastricht University Medical Center
Sample preparation
GROUPS 5-8
Frans Ramaekers
Dept. Molecular Cell Biology, Maastricht University Medical Center
Sample preparation
GROUPS 1-4
UNS 50
K.0.480
UNS 50
K.0.480
UNS 50
K.0.480
Frans Ramaekers
Dept. Molecular Cell Biology, Maastricht University Medical Center
Demonstrations (according to schedule, 45 min per group)
GROUPS 1-4
Confocal scanning laser microscopy
14.00 – 17.00
Location
UNS 50
K.0.480
Jos Broers / Helma Kuijpers
Dept. Molecular Cell Biology, Maastricht University Medical
Center
Demonstrations (according to schedule, 45 min per group)
GROUPS 5-8
Confocal scanning laser microscopy
Jos Broers / Helma Kuijpers
Dept. Molecular Cell Biology, Maastricht University Medical
Center
NVvM
UNS 50
Level 5
UNS 50
Level 5
Wednesday, June 10, 2015
Time
09.00 – 09.45
Item
Analysis of the neuromuscular junction – quantitative microscopy
09.45 – 10.30
Mario Losen
Dept. of Basic Neurosciences, Maastricht University Medical Center
Backgrounds and applications of STED Microscopy
Patrick van Wieringen
Leica Microsystems BV
Location
UNS 40
A.0.771
UNS 40
A.0.771
10.30 – 10.45
Coffee/tea break
10.45 – 11.30
Two-photon laser microscopy for the three-dimensional study of
tissues
UNS 40
A.0.771
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
Image Processing with Huygens and ImageJ
GROUPS 5-7
UNS 40
A.0.771
Jos Broers / Remko Dijkstra
Dept. Molecular Cell Biology, Maastricht University Medical Center
Scientific Volume Imaging bv
Image Processing with Huygens and ImageJ
GROUPS 1-4
UNS 40
A.0.771
11.45 – 13.15
15.00 – 16.30
11.45 – 17.00
Jos Broers / Remko Dijkstra
Dept. Molecular Cell Biology, Maastricht University Medical Center
Scientific Volume Imaging bv
Demonstrations (according to schedule, 45 min per group)
GROUPS 1-7
Two-photon scanning laser microscopy
Timo Rademakers, Dimitris Kapsokalyvas [Zhuojun Wu]
Sanquin, Amsterdam
NVvM
UNS 50
Level 0
Thursday, June 11, 2015
Time
09.00 – 10.00
10.00 – 11.00
Item
Advances in multiphoton microscopy – in vivo imaging in
cardiovascular research
Location
UNS 40
A.0.771
Timo Rademakers
Sanquin, Amsterdam
High throughput and high content cellular analysis for systems
biology
UNS 40
A.0.771
Winnok De Vos
Ghent University
11.00 – 11.15
Coffee/tea break
11.15 – 12.15
Advanced Electron Microscopy
12.15 – 13.30
13.30 – 14.00
Peter Peters
Maastricht MultiModal Molecular Imaging Institute(M4I),
division of Nanoscopy
Lunch break
Intro to Nanoscopy Tour
14.00 – 17.00
Peter Peters
Maastricht MultiModal Molecular Imaging Institute(M4I),
division of Nanoscopy
Nanoscopy Tour
NVvM
UNS 40
A.0.771
UNS 40
A.0.771
UNS 50
Level 0
Maastricht Microscopy Meeting (M3) | Friday, June 12, 2015
Symposium Advanced Microscopy and Vital Imaging
Greepzaal, level 4, azM | Maastricht University Medical Center, P. Debyelaan 25, Maastricht
09.30 – 10.00
Arrival & Coffee
10.00 – 10.10
Welcome by Marc van Zandvoort
Dept. of Molecular Cell Biology, Maastricht University Medical Center
10.10 – 10.55
Ron Heeren
Maastricht Multimodal Molecular Imaging Institute
Visualizing signals of health and disease with imaging mass spectrometry
10.55 – 11.40
Grazvydas Lukinavicius
Laboratory of Protein Engineering, Ecole Polytechnique Fédérale de Lausanne
Applications of silicon-rhodamine for the imaging of living cells
11.40 – 12.25
Go van Dam
Dept. of Surgical Oncology, University Medical Center Groningen
In vivo fluorescence immunohistochemistry in humans: a new branch on the tree in drug
development
12.30 – 13.45
Lunch
13.45 – 14.30
Gert-Jan Bakker
Dept. of Cell Biology, Radboud Institute for Molecular Life Sciences, Nijmegen
Third harmonic generation for intravital microscopy of the tumor stroma
14.30 – 15.15
Morgan Beeby
Dept. of Life Sciences, Imperial College, London
Structure and evolution of rotary motors
15.15 – 16.00
Victoria Ridger
Dept. of Cardiovascular Science, University of Sheffield
A novel role for neutrophils through the release of microvesicles
16.00 - 16.45
Speaker 7
To be announced
16.45 – 17.30
Closure & Drinks
Participation is free, but please note that registration is obligatory. You can register by sending an e-mail, including your
name and department, to the secretary of Molecular Cell Biology, Maastricht University Medical Center.
Email secr-mcb@maastrichtuniversity.nl
Telephone +31-43-3881351
NVvM