Water structures
carrying DNA
information,
Application to
HIV/AIDS and Autism
L.Montagnier, Borovets, October 23, 2013
The Evolution of
Medicine
Toward 4 P Qualities
• P1 Preventive
• P2 Predictive
• P3 Personalized
• P4 Participative
Emergence or reemergence
of new epidemics due to :







Globalization of exchanges and travels
Demography: concentration in large cities
Nutrition (pesticides, water)
Environmental factors
Climate changes, electromagnetic radiations
Contacts with wild and farm animals
Decline of immune defenses
The most important burden
Chronic Diseases
- Cancers
- Cardiovascular
- Neurodegenerative
- Arthritic
- Autoimmune
- Multifactorial,
but in common:
oxidative stress
infectious agents (?)
Various environmental factors effects
accumulate
Radiations (, X, UV, visible, hertzian)
+
+
+
+
+
+
+
+
+
Air chemical pollution
Inadequate Food
Excessive physical exercize
tobacco smoke
alcohol
ischemia
Parasitic infections
Bacterial infections
Viral infections
= DISEASE
Pathological effects of Oxydative Stress
 Lipid hyperoxydation (plasma, cell membranes)
 Protein Oxydation --> destruction, agregates
 DNA oxydation --> Mutations, Chromosomal
Breakage
Oxidative stress
Weakens the immune system
Activates transcription factors
(NF-kappa B)
Activates genes involved
in cell division, inflammatory cytokines,
lymphocytes activation
Immune dysfunction, apoptosis
(TH1 TH2)
The Human Genome
Genes (+ introns +promoters)
Retroelements: retroviruses
retrotransposons
LTR
Total
3%
8%
13%
23%
47%
Repeats
50%
LINE
SINE
Unknown
22%
13%
?
Our microbial ecosystem :
Mucosa
Skin
Constant exposure to
microbial agents and
immune protection
Extreme genetic plasticity
of microorganisms:
 virus : HIV, Influenza
 Bacteria (plasmids)
 Parasites (retrotransposons)
against reaction of the immune system
How bacteria have learned how to
persist despite the immune system
- Biofilms
- Mobile antibiotic resistant genes
- Nanoforms
- Nanostructures
- Endosymbiontes
Parasite
Bacterium
(Worm)
intracellular
Filarial nematode
Wolbachia
Virus
Persistent cold infections
•Inability of the immune system to eradicate them
(tolerance, oxidative stress)
•Non-multiplicative forms of « classical » bacteria
•intracellular bacteria
•Sanctuaries
(bone marrow, joints, intestine, brain, ….)
•Vectors (Parasites)
•Remote effects (toxin, nanostructures)
Antibiotics affect them only when they go out of sanctuaries
EXPLORING THE ROLE
OF LATENT INFECTION
IN CHRONIC DISEASES
A physical and molecular approach
Two technologies for detecting
bacteria and viral DNA’s
•A new technology based on the
production of electromagnetic waves
•The Polymerase Chain Reaction
(PCR) using the 16S ribosomal DNA
variability
Classical model of PCR
oligo
Taq
polymerase
oligo
A newly discovered property
of DNA :
Resonance emission of low frequency
electromagnetic waves by high water dilutions
of DNA.
Molecular recognition
without close contact
(by waves and resonance)
Capture of the signals
Sample
X 500
Sensor coil
Amplifier
Signal Analysis
software
Computer
FACTS
Detection of Ultra Low Frequencies
Waves (ULF 500-2000 hertz) in certain
dilutions of filtrates (100nM, 20nM,
15nM)
from
cultures
of
microorganisms (virus, bacteria) or from the
plasma of humans infected with the
same agents.
7-100 Hz
Filtrati
on 0.1
0.02
2ng/1ml
16
10-2 10-3 10-4
10-5 10-6 10-7 10-8 10-9 10-10 10-12 10-13 10-14 10-15 10-
1000 3000 Hz
Amplitude
Noise
(+)
Time (sec)
Spectral Frequency Analysis
Fourier
Tranformation
A positive signal is defined by:
amplitude increase
Shift to higher frequencies (500-2000 Hertz)
Noise
(+)
Frequency (1-20000 Hertz)
Suspensions of pure culture of bacteria with pathogenic
potential are producing electromagnetic signals (EMS) in the
range of dilution usually 10-8 10-13, sometime (E.coli) up to
10-18
They are: E.coli, Streptococcus, Salmonella, Staphylococcus,
B.subtilis, Clostridium, Pseudomonas, etc.
Micro-organisms involved
in EMS induction
1.DNA from main pathogenic bacteria
Streptococcus
Staphylococcus
Pseudomonas
Mycoplasma pirum
Salmonella
Clostridium
Proteus mirabilis
B. Subtilis
Borrelia burgdorferi
Sutterella
- From viruses
HIV1
Influenza group A
HBV
HCV
- Genes involved
M.pirum adhesin
HIV genes
Interpretation
10-2
DNA
Water excitated state
crystal like gel
no EMS
10-9
Free polymer
self maintained
by EMS
I – DNA’s emit EMS
II – EMS are produced by water nanostructures (naneons)
III – EMS are producing naneons
IV – Naneons and EMS carry specific DNA information
EMS are produced by water
nanostructures (naneons)
Evidence :
from filtration

Size : between 20 and 100 nM for bacterial sequences

Smaller that 20µM for viral sequences
from biophysical studies

indicating spectrometral changes in the dilutions
producing EMS
Naneons and EMS carry
specific DNA information
Natural and digital transmission
Classical model of PCR
oligo
Taq
polymerase
oligo
PCR on water nanostructures
oligo
Taq
EMS
oligo
DNA
Water
Naneons
EMS
EMS
Water
Naneons
7 Hz
PCR
DNA
Generator 7Hz
DNA
-6
-6 water
Tube 1
Tube 2
µmetal
-2
-3
-4
-5
-6
18hrs
water
+ + +
EMS
D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM
Transmission in water of D-4 LTR HIV DNA (104bp) 7Hz, 18 Hrs and then PCR (35 cycles) from D-2 to D-15 after filtration 450 and 20 nM
DW: Distilled Water / FD2: Dilution 10-2 after filtration 450and 20 nM
Water-mediated photonic transmission of DNA
DNA
Water
Naneons
EMS
Computer
Digitized
Receiver
EMS
Analog
PCR
Computer
Water
Naneons
DNA
Reproduction of DNA transduction
in other laboratories
File EMS of 194 bp DNA from HIV1 LTR
Sent to Benevento University,
Molecular Biology Laboratory
DNA reproduced and sequenced
100 % identical to original
File EMS of 499 bp DNA from Borrelia burgdorferi
Sent to Laboratory of Chronix Biomedicals
University of Gottingen
Molecular Biology Laboratory
DNA reproduced and sequenced
100 % identical to original
File EMS of 499 bp DNA from Borrelia burgdorferi
Sent to Laboratory of Chronix Biomedicals
University of Gottingen
Water-mediated photonic transmission of DNA
Gel electrophoresis of the PCR DNA product (Borrelia Burgdorferi)
E.Schutz et al. Goettingen, 2011
ELF
DNA
WATER
(plasma)
DNA
DNA
DNA
DNA
How pathogenic information can persist,
and escape immune defence and treatment.
Two main problems for AIDS research
in 2013
- The viral DNA reservoir
- The Higher sensitivity of African
people to HIV infection
The dual origin of EMS in HIV/AIDS
EMS
HIV
 < 20 nM
Agent X  < 100 nM
plasma RBC
++
+/-
-
++
Evidence that the agent X is a bacterium
- Presence of a sequence close to 16 S ribosomal DNA
of Rickettsiales
- Sensitive to antibiotics ( in vitro and in vivo)
- Growth in cell line
- Electron microscopy
- Associated with Red Blood Cells
Human sequences associated with
the agent X
237 bp (human chromosome 1)
213 bp (human chromosome 7)
and more…
Strong correlation with HIV infection
- Contains human DNA sequences emitting EMS
only in pathogenic HIV infection
-Sequence 213 bp from Chromosome 7
 EMS in HIV + patients
 no EMS in HIV - patients
DNA
HIV plasma
RBC
Risk factor 2
RBC
Risk factor 1
RBC
PCR
EMS
+
+
(LTR 195 bp)
(all HIV)
(20 nM)
+
+
+
-
(100 nM)
(213/237 bp)
( all HIV+, a few HIV-) (HIV+)
( all HIV+, many HIV-)(HIV- & HIV+)
(100 nM)
RISK FACTORS OF HIV INFECTION
CONCLUSION 1
« A new intracellular bacterium has
been found in human red blood
cells. A variant bearing extra DNA
sequences is present in all HIV
infected patients and also in some
healthy HIV negative African
individuals”.
CONCLUSION 2
Origin of EMS :
not necessarily depending on DNA sequence
- depending on transmissible modification of DNA
(free radicals of water ?)
- Association with pathogenicity
AUTISM :
THE GUT, BLOOD and BRAIN
MICROBIAL CONNECTION
GUT
BLOOD
BRAIN
% EMS/noise
Correlation between EMS, antibiotic treatment
and clinical signs in an autistic child
40 CEFUROXIM
30 -
20 -
Starts speaking
Accept various food
More present with
Family members
AZITHROMYCIN
10 -
0-
27
50
100
133
days
Environment
al factors
genetic
susceptibility
Oxidative
stress
immunosuppres
sion
Bacterial
agents
Oxidative Somatic
stress
mutations
« prion »
effect
Reversible
Less and less
reversible
In press
Electromagnetic detection of HIV DNA in the blood of AIDS patients
treated by antiretroviral therapy.
Luc MONTAGNIER†×^, Jamal AISSA†, Claude LAVALLEE×,
Mireille MBAMY⁰, Joseph VARON#, and Henri CHENAL⁰
×
World Foundation for AIDS research and Prevention (UNESCO), 1 rue Miollis, 75015, Paris, France
† Nanectis Biotechnologies, France
⁰ CIRBA Centre Intégré de Recherches Biocliniques d’Abidjan, Ivory Coast
# The University of Texas Health Science Center, Houston, USA.
Abstract : Electromagnetic signals of low frequency have been shown to be durably produced in aqueous dilutions of the
Human Imunodeficiency Virus DNA. In vivo, HIV DNA signals are detected only in patients previously treated by
antiretroviral therapy and having no detectable viral RNA copies in their blood. We suggest that the treatment of AIDS
patients pushes the virus towards a new mode of replication implying only DNA, thus forming a reservoir insensitive to
retroviral inhibitors. Implications for new approaches aimed at eradicating HIV infection are discussed.
Key words: DNA, Electromagnetic signals, bacteria
Centre Intégré de Recherche Bioclinique d’Abidjan
World Foundation of AIDS
Research and Prevention
R. Olivier, Cl. Lavallee,
H.Chenal, M. Mbamy,
Nanectis Biotechnologies SA
J.Aissa, Cl.Lavallee, R.Olivier
Goettingen University and Chronix
Biomedicals
E. Schutz, H.Urnovitz
University of Washington, Seattle
Gerald H. Pollack
“Absence of evidence
is not
evidence of absence”
Carl Sagan