Teresa Audesirk • Gerald Audesirk • Bruce E. Byers Biology: Life on Earth Eighth Edition Chapter 5 Membrane Structure and Function Copyright © 2008 Pearson Prentice Hall, Inc. Chapter 5 Opener Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Chapter 5 Opener (inset) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. extracellular fluid (outside) carbohydrate receptor protein recognition protein cholesterol phospholipid binding site glycoprotein phospholipid bilayer protein transport pore protein protein filaments cytosol (inside) Figure 5-1 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. tails (hydrophobic) head (hydrophilic) Figure 5-2 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. extracellular fluid (watery environment) hydrophilic heads phospholipid hydrophobic tails bilayer hydrophilic heads cytosol (watery environment) Figure 5-3 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. more fluid Figure 5-4 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. less fluid (outside) (inside) reactions Figure 5-5 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 1 A drop of dye is placed in water. 2 Dye molecules diffuse into the water; water molecules diffuse into the dye. drop of dye water molecule Figure 5-6 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 3 Both dye molecules and water molecules Are evenly dispersed. Table 5-1 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (a) Simple diffusion through the phospholipid bilayer lipid-soluble molecules (extracellular fluid) and O2, CO2, H2O O2 (cytosol) Figure 5-7a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (b) Facilitated diffusion through a channel protein Cl– H2O, ions Cl– Proteins form a hydrophilic channel. Cl– Cl– channel protein Cl– (cytosol) Figure 5-7b Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (c) Facilitated diffusion through a carrier protein carrier protein amino acids, sugars, small proteins (extracellular fluid) (cytosol) 1 Carrier protein has binding site for molecule. 2 Molecule enters binding site. 3 Carrier protein changes shape, transporting molecule across membrane. Figure 5-7c Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 4 Carrier protein resumes original shape. isotonic: no net flow of water Figure 5-8 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Figure E5-1 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. egg without aquaporins Figure E5-2a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. egg with aquaporins extracellular fluid water aquaporin channel cytosol water Figure E5-2b Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Figure 5-9 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (a) Isotonic solution Equal movement of water into and out of cells. (b) Hypertonic solution Net water movement out of cells. Figure 5-10 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (c) Hypotonic solution Net water movement into cells. cytosol central vacuole cell wall When water is plentiful, it fills the central vacuole, pushes the cytosol against the cell wall, and helps maintain the cell's shape. Water pressure supports the leaves of this impatiens plant. Figure 5-11 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. plasma membrane When water is scarce, the central vacuole shrinks and the cell wall is unsupported. Deprived of the support from water, the plant wilts. cytosol central vacuole When water is plentiful, it fills the central vacuole, pushes the cytosol against the cell wall, and helps maintain the cell's shape. Figure 5-11 (part 1) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Water pressure supports the leaves of this impatiens plant. Figure 5-11 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. cell wall plasma membrane When water is scarce, the central vacuole shrinks and the cell wall is unsupported. Figure 5-11 (part 3) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Deprived of the support from water, the plant wilts. Figure 5-11 (part 4) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 1 The transport protein binds both ATP and Ca2+. 2 Energy from ATP Changes the shape of the transport protein and moves the ion across the membrane. 3 The protein releases the ion and the remnants of ATP (ADP and P) and closes. (extracellular fluid) ATP ATP recognition site Ca2+ ADP (cytosol) ATP binding site Figure 5-12 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. P (a) Pinocytosis (extracellular fluid) 1 3 2 (cytosol) vesicle containing extracellular fluid 1 A dimple forms in the plasma membrane, which 2 deepens and surrounds the extracellular fluid. 3 The membrane encloses the extracellular fluid, forming a vesicle. Figure 5-13a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (b) Pinocytosis in a smooth muscle cell. 1 extracellular fluid 2 cytosol Figure 5-13b Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 3 Receptor-mediated endocytosis nutrients (extracellular fluid) receptors coated pit 1 2 3 (cytosol) 4 coated vesicle 1 Receptor proteins for specific molecules or complexes of molecules are localized at coated pit sites. 2 A vesicle (“coated vesicle”) containing the bound molecules is released into the cytosol. 3 The coated pit region of the membrane encloses the receptor-bound molecules. 4 The receptors bind the molecules and the membrane dimples inward. Figure 5-14 (part 1) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. extracellular particles bound to receptors coated vesicle (extracellular fluid) (cytosol) 1 2 3 4 0.1 micrometer protein coating coated pit plasma membrane Figure 5-14 (part 2) Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (a) Phagocytosis food particle pseudopods 1 (cytosol) (extracellular fluid) 2 food vacuole 3 1 The plasma membrane extends pseudopods toward an extracellular particle (for example, food). 2 The ends of the pseudopods fuse, encircling the particle. 3 A vesicle called a food vacuole is formed containing the engulfed particle. Figure 5-15a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (b) Amoeba An Amoeba (a freshwater protist), engulfs a Paramecium using phagocytosis. Figure 5-15b Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (c) White blood cell A white blood cell ingests bacteria using phagocytosis. Figure 5-15c Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. secreted material plasma membrane (extracellular fluid) vesicle (cytosol) Material is enclosed in a vesicle that fuses with the plasma membrane, allowing its contents to diffuse out. Figure 5-16 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 0.2 micrometer r r distance to center (r) surface area (4r2) volume (4/3r3) surface area/volume 1.0 12.6 4.2 3.0 Figure 5-17 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. 3.0 113.1 4.2 1.0 (a) Desmosome small intestine cells lining small intestine (b) Tight junction urinary bladder cells lining bladder microvilli desmosome plasma membranes (edge view) Protein strands hold cells together. Tight junctions formed by strands of protein seal cells together. protein filaments in cytosol Figure 5-18 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. (a) Gap junctions (b) Plasmodesmata root liver liver cells root cells plasma membrane Gap junctions: pairs of channels connect insides of adjacent cells. Figure 5-19 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. cell wall plasma membrane Plasmodesmata connect insides of adjacent cells. Figure 5-20 Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Figure 5-21a Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc. Figure 5-21b Biology: Life on Earth 8/e ©2008 Pearson Prentice Hall, Inc.