Genetic epidemiology of cystic fibrosis in Saguenay-Lac-St. Jean (Quebec, Canada) Daigneault J, Aubin G, Simard F, DeBraekeleer M Clinical Genetics April 1991 Presented by Soodtida Tangpraphaphorn Introduction • Descriptive case-control genetic epidemiological study – Quantifies incidence, prevalence, carrier rates – Some EO associations, no causality established • Makes etiological hypotheses, suggests associations and causal relationships for future study • Study population shows elevated prevalence of CF compared to overall population at risk. • Inbreeding slightly higher in CF group compared to controls due to remote consanguinity. • Endogamy not higher in CF group than controls. Clinical Cystic Fibrosis • Genetic respiratory illness with pleiotropy – Most commonly affects populations of NW European descent – Causes airway mucus to thicken, resulting in chronic respiratory obstruction and infections – Impairs pancreatic exocrine function – Complications can decrease lifespan – Diagnosed by sweat test Cellular Cystic Fibrosis • Caused by mutation in CFTR gene – Most commonly DF508 • Mutation results in defective protein product – Protein is a cell membrane ion channel • Defective protein affects chloride anion transport in/out of cell –Ion channel fails to export to cell membrane –Ion channel is in cell membrane, but cannot function Saguenay-Lac-St.Jean • 200km NE of Quebec City • Inlet of St. Lawrence Seaway • Very geographically remote • Southernmost fjord in the world SLSJ Epidemiological Map Study Population • Pop. 285,100 (1986); 98% French-speaking Catholics • Isolation – Settled in 1840s, migrated from Charlevoix – Founder effects • Mutation frequencies different from urban Canadian population • Particularly interesting to geneticists – Elevated carrier rates and prevalence of many different genetic disorders Study Population • 127 CF cases in SLSJ –125 patients from CF clinic at Chicoutimi, 2 from Quebec City • 3 groups population-based controls –Not described in this study • Requires readers to refer to investigators’ other studies Methods • Study time-frame (1975-1988) • Extract demographic information from database of CF cases in SLSJ • Migration histories from subjects and their families • Calculate prevalence & incidence from birth statistics and case data • Estimate carrier rate via Hardy-Weinberg equation Results • Number of CF live births = 78 • Total live births = 70351 • CF incidence (#cases/#at risk) = 1 per 902 – Incidence for all Canadian whites = 1 per 2500 – Secular trends show stable annual IR • Hardy-Weinberg carrier rate = 1 per 15 • Stronger kinship and more inbreeding in CF group Discussion • SLSJ population not isolated for long periods of time – High prevalence may be attributable to mutations before migration from Charlevoix – Migration of large founder group brought many alleles into population • Different frequency of mutations • Good descriptive epidemiology – Study stands alone well, better when taken in context Prevention Recommendations • Primary – increase genetic diversity by mating outside of population • Secondary – screen locals for genetic mutations and offer genetic counseling with family planning, incl. PGD • Tertiary – early detection in infants, more social-medical services, close medical supervision References 1. 2. 3. 4. Daigneault J, Aubin G, Simard F, DeBraekeleer M. Genetic epidemiology of cystic fibrosis in Saguenay-LacSt. Jean (Quebec, Canada). Clinical Genetics. 1991;40:298-303. Rozen R, DeBraekeleer M, Daigneault J, Ferreira-Rajabi L, Gerdes M, Lamoureaux L, Aubin G, Simard F, Fujiwara TM, Morgan K. Cystic fibrosis mutations in French Canadians: three CFTR mutations are relatively frequent in a Quebec population with an elevated incidence of cystic fibrosis. American Journal of Medical Genetics. 1992;42:360-364. DeBraekeleer M, Daigneault J. Spatial distribution of the DF508 mutation in cystic fibrosis. Human Biology. 1992;64:167-174. Daigneault J, Aubin G, Simard F, DeBraekeleer M. Incidence of cystic fibrosis in Saguenay-Lac-St. Jean (Quebec, Canada). Human Biology. 1992;64:115-119. Cause Celebrè