Chapter 9- Patterns of Inheritance • • • • • • • • • • • • • • • • • • • • ABO blood groups Alleles Amniocentesis Carrier Chorionic villus sampling Chromosome theory of inheritance Codominance Cross Cross-fertilization Cystic fibrosis Dihybrid cross Dominant allele F1 generation F2 generation Genetics Genotype Hemophilia Hermaphroditic Heterozygous Homozygous • • • • • • • • • • • • • • • • • • • • • • Hybrids Incomplete dominance Linked genes Monoecious Monohybrid cross P generation Pedigree Phenotype Pleiotropy Polygenic inheritance Principle of independent assortment Principle of segregation Punnett square Recessive allele Recombination frequency Rule of addition Rule of multiplication Self-fertilize Sex chromosomes Sex-linked genes Testcross Ultrasound imaging Genetics • Study of heredity Gregor Mendel • Monk, father of modern genetics, 1860’s • Discovered genetic principles by meticulous breeding of pea plants • Used peas because they were readily available, easy to grow, had many distinguishable traits, could control mating Pea Traits Genetic Cross Vocabulary • Self-fertilize- sperm from pollen fertilizes egg containing carpel • Cross-fertilization- fertilizing one plant from pollen of another plant – Offspring produced are hybrids • P generation (parent) • F1 generation – offspring of P • F2 generation- offspring of F1, can be self- or crossfertilized Genetic Cross Vocabulary con’t • Monohybrid cross- parents differ in only one trait • Alleles- alternate forms of a gene A (dominant) or a (recessive) • Homozygous- alleles for a trait are identical, AA • Heterozygous- alleles are different, Aa • Phenotype- organism’s physical trait expressed – Ex: purple flower • Genotype- genetic make up of trait – Ex: PP • Alleles for a gene are at the same locus (point) on homologous chromosomes Mendel’s hypotheses: • There are alternative forms of genes (units that determine traits) • For each characteristic- an organism has a gene from each parent, they can be the same allele or different • Sperm and egg each have 1 allele for a trait • Idea of dominant and recessive alleles Mendel’s Principle of Segregation • Pairs of genes segregate (separate) during gamete formation (what is that process called?) – gametes fusing during fertilization regain pairs of genes Mendel’s Principle of Independent Assortment • Each pair of alleles segregates independently during gamete formation • Dihybrid cross- cross between parents that differ in 2 traits Mendel’s principles reflect probability How can we determine an unknown genotype? • Testcross- mating between an unknown individual and a homozygous recessive individual Rule of multiplication- probability that a compound event is the product of the separate probabilities of the independent events Ex: b from mom (1/2), b from dad (1/2) baby being bb ½ x ½ = ¼ Rule of addition- probability that an event can occur in 2 or more alternative ways is the sum of the separate probabilities of different ways Ex: in Punnet square Bb = ¼ the other Bb is ¼ probability of being Bb is ¼ + ¼ = 2/4 What about humans? • To find human inheritance information: – Collect as much family data as possible – Construct pedigree chart – At first heterozygotes are not known, must determine phenotypes of offspring then could lead to information on carriers (heterozygotes) Chart Key Pedigree Chart • Can you figure out carriers and genotypes from this pedigree? Many genetic disorders are a result of a single gene – Most are recessive • family may not know of defect until offspring with two recessive alleles is born • Ex: CF cystic fibrosis – Dominant traits are not always prevalent just because they are dominant, usually due to lethality of disorder (death before reproduction) • Ex: polydactyly, achondroplasia How can we detect genetic disorders? • Fetal testing – Amniocentesiscells from amnionic fluid is tested – CVS- chorionic villi sampling- fetal tissue from placenta is tested – Ultrasound/sonogram- uses sound waves to product picture of fetus • Genetic screening – Most test DNA but some test enzymes – Most tell risk of disorders that depend on multiple genes – Types of testing • • • • Carrier- determine if you are a carrier of a harmful allele Diagnostic- confirm or rule out disorder Prenatal- checks for disorders in fetuses Newborn- catches inherited disorders quickly so medical attention can be given, “heel prick” • Predictive- test at any time to determine risk for developing disorder – Ex: BRAC1 and 2- linked to breast cancer, Huntington’s disease Variations of Mendel’s Principles Genotype/phenotype relationship is not always straightforward • Incomplete dominance – produces intermediate phenotypes • Ex: hypercholesterolemia • HH= normal, Hh=slightly affected, hh=has disease severely • Codominance – when both alleles are expressed in the phenotype • Ex: ABO bloodgroups • Some genes have more than 2 alleles, each individual can only have 2 • A, B, AB or O bloodgroups –AB is codominant • Pleiotrophy – impact of single gene on more than one characteristic • Ex: sickle-cell disease – abnormal hemoglobin molecules are produced • Polygenic inheritance – affect of 2 or more genes on a single phenotype trait • Ex: human skin color, height Other genes are on sex chromosomes • Sex chromosomes- determine sex in species – Humans- X and Y are sex chromosomes • Each have 44 autosomes (22pairs) and 1 pair of sex chromosomes XX-female or XY- male • Males determine sex of offspring because they can pass X or Y, females only pass X • SRY gene on Y chromosome triggers testes development, without it, ovaries are developed • Fruit flies- # of X’s determine male or female, however presence of Y is essential for sperm production Sex chromosomes con’t – X-O system- grasshoppers, crickets, roaches • Female- XX male- XO (O=absence of chromosome) – Z-W system- fish, butterflies, birds • Males-ZZ females-ZW, eggs determine sex – Determination by chromosome #- ants and bees • Females- diploid- develop from fertilized eggs • Males- haploid- develop from unfertilized eggs Some can produce both sperm and eggs • Monoecious- plants that produce sperm and eggs – ex: corn • Hermaphroditic- animals that produce sperm and eggs – ex: earthworms Sex- linked genes • Genes located on sex chromosomes – Not related to sex determination – Mostly found on X – In humans X-linked recessive traits mostly affect males – Females are carriers and pass to sons, sons have no other X to dominate over the recessive allele • Ex: colorblindness, hemophilia Chromosome behavior accounts for Mendel’s principles: • Chromosomal Theory of Inheritance – Genes are located on chromosomes, the behavior of chromosomes (segregation and independent assortment- Mendel’s ideas) during meiosis and fertilization accounts for the inheritance patterns Linked Genes • Don’t follow Mendel’s rules • If genes are located close together they tend to be inheritted together Linked Genes con’t • Data from crossing over can lead to mapping genes, farther apart they are greater chance of crossing over in between the genes • Crossing over accounts for new gene combinations – Morgan worked with fruit flies to develop: – Recombination frequency- % of recombinants in offspring Gene Map