Midterm-Review Meeting
Molecular MachinesDesign and Nano-Scale Handling of Biological Antetypes and Artificial Mimics
Work Package 6: Biomolecular motors on nanostructures
Objectives
Construct kinesin-motor based biological-inorganic hybrid
devices for controlled nano-transport processes.
Partners
ICG-GC
TU/d-MB
EPFL
ETH
PM
30
30
24
6
Specific Targeted Research Project (STREP) in FP6-NMP-2002
Midterm-Review Meeting
Work Package 6: Biomolecular motors on nanostructures
The molecular motors
How do they work ?
Energy conversion
Cytoskeleton motors : Kinesin,
Myosin...
(M6-1)
Structural changes vs. ATP hydrolysis
High resolution techniques
f-actin
Myosin
Microtubules
How include the motors in nano-devices ?
(D6-1) Hybrid organic/inorganic systems
Nano-handling
Kinesin
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Traveling Wave Tracking
Work Package 6: Biomolecular motors on nanostructures
I (t )  I 0 cos0t   ( x, t )
Detection
APD
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Traveling Wave Tracking
Work Package 6: Biomolecular motors on nanostructures
Piezo: 10 nanometers steps
Single 10 nm step
RMS noise ± 2 Å
-
+
Many motors ?
Time resolution ~ 2 µs
RMS noise ~ 2 Å
-Drift ~ 1 nm/s +
Single motor ?
Midterm-Review Meeting
Work Package 6: Biomolecular motors on nanostructures
T = 300 K
kx = 0.3 pN/nm
xx = 7.4 pN ms/nm
Average speed = 500 nm/s
Backward steps ~ 10 %
Step time scale < 70 µs
Midterm-Review Meeting
Work Package 6: Biomolecular motors on nanostructures
Structures designed with a 3D
approach for the TWT setup (collaboration with the Institut Curie)
3D approach
In order to observe 3D movements, including
rotations, the object of study has to be suspended
Some samples
Soft Lithography approach
Glass
Midterm-Review Meeting
Work Package 6: Biomolecular motors on nanostructures
Nanostructures for
kinesin-driven microtubule motility
Rectification, confinement and electrical docking
Time sequence
of microtubule
docking
Midterm-Review Meeting
Work Package 6: Biomolecular motors on nanostructures
Rectification of
kinesin-driven microtubule motility
SEM image of rectifying structure
Fluorescence image of microtubule
motility in nanostructures
Rectifier geometries
92 % efficiency in rectification
Midterm-Review Meeting
Work Package 6: Biomolecular motors on nanostructures
Production
M6-1 M6-2
and D6-1
Resources
additional
projects
Conclusion
Van den Heuvel, M. G. L.; Butcher, C. T.; Lemay, S. G.; Diez, S.; Dekker, C. Nano Lett. 2005, 5, 235-241.
Van den Heuvel, M. G. L.; Butcher, C. T.; Smeets, R. M. M.; Diez, S.; Dekker, C. Nano Lett. 2005, 5, 1117-1122.
L. Busoni, A Dornier, J-L Viovy, J. Prost, G. Cappello; Journal of Applied Physics 2005, 98
A. Dupont, C. Symonds, L. Busoni, J. Prost, G. Cappello; In preparation (2005)
Many different nanofabricated structures were made in TU-Delft
New flexible optical technique, called Travelling Wave Tracking (ICG-GC).
Microtubules injected in the structures (TU-Delft) and the activity has successfully been checked
First experiments on single molecular motor
Post-Docs: Irene Dujovne, Clémentine Symonds, Lorenzo Busoni
Conferences and Meetings
New motors: Myosin V and VI (collaboration with J. Spudich, Stanford)
Gold nanoprobes (collaboration with R. Levy, University of Liverpool)
DNA associated motors
Objectives for the first part of the project have been achieved and we can begin measuring single
molecular motors moving on the suspended microtubules.
Specific Targeted Research Project (STREP) in FP6-NMP-2002