LUMC: Human and Clinical Genetics / Leiden Genome Technology Center Introduction to NGS Data Analysis May 22, 2014 Location: LUMC, Leiden Given by: Michiel van Galen, Martijn Vermaat, Jeroen Frank and Jeroen Laros. The course aims at introducing participants (with no or little experience) to NGS data analysis. In the morning we introduce general data analysis techniques, like alignment and de novo assembly. In the afternoon we discuss some applications and good research practices. Program: 09:00 - 09:30 09:30 - 10:00 10:00 - 10:30 Introduction Quality control Alignment 10:30 - 11:00 Break 11:00 - 11:30 11:30 - 12:00 Variant calling De novo assembly 12:00 - 13:00 Lunch 13:00 - 13:30 13:30 - 14:00 14:00 - 14:30 14:30 - 15:00 Phylogenetic reconstruction Plasmid and gene profiling Good research practices for data analysis Discussion Jeroen Laros Michiel van Galen Martijn Vermaat Martijn Vermaat Jeroen Frank Michiel van Galen Jeroen Laros Michiel van Galen