A study of walnut effect on sex organs weights and morphological
parameter in diabetic adult male Rats induced by Streptozotocin (STZ)
Rostam Ghorbani*1, Mitra bakhtiari1, Tahmineh Mokhtari**2, Cyrus Jalili1, Mozafar Khazaei1
1-PhD of Anatomical sciences, department of anatomy, Kermanshah University of Medical Sciences
1-PhD Student of Anatomical sciences, department of anatomy, Tehran University of Medical Sciences
*Corresponding author: Rostam Ghorbani (rostamgh@yahoo.com, 09181317432.)
**Presented by: Tahmineh Mokhtari
Objectives
Results
600
.
Blood Suger (mg/dl)
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400
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$$$
$$$
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BS 1
***
300
BS 2
BS3
200
100
0
Sham
Control
Walnut 6%
Walnut 9%
Walnut 12%
Figure 1. walnut effect on Blood sugar in diabetic adult male Rats
0.03
Seminiferous diameter (µm)
Diabetes mellitus is a degenerative disease
that has deleterious effects on male
reproductive function, possibly through an
increase in oxidative stress. Walnut is
known as an antioxidant that may be
effective on decreasing free radicals. The
aim of this study was to evaluate walnut
consumption effects on testis and prostate
in diabetic Wistar male rats induced by STZ.
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500
0.025
*
0.02
0.015
0.01
0.005
Methods
0
Sham
Control
walnut6% walnut 9%
walnut
12%
Figure 2. walnut effect on seminiferous diameters in diabetic adult male Rats
Conclusions
0.7
Our data showed that, walnut as an
antioxidant source had a significant
improvement effects on reproductive system
References
as testis and prostate
in diabetes-induced
male rats.
prostat weight
0.6
0.5
0.4
***
0.3
***
***
***
0.2
0.1
0
1.6
Sham
Control
1.4
Walnut 6%
Walnut 9%
Walnut 12%
Exprimental1.6groups
1.2
*
*
1.4
*
1
Right testis weight
left testis weigth (mg)
Diabetes mellitus was induced by STZ
(60mg/kg) in wistar male rats. Rats were
randomly divided in 5 groups (6 rats in each
group) included: normal diet and healthy
(sham), Diabetic by normal diet (control)
and Diabetic by 6, 9 & 12% walnut
(experimental groups) in their diet. Testis
weight, prostate weight and seminiferous
tubules diameters were evaluated for each
groups.
Blood sugar was significantly increased in
three times of testing. Prostate and right
testis weight significantly decreased in
experimental and control groups compare to
sham group. Left testis weight significantly
decreased in experimental (6 & 9%) and
control groups compare to sham group. In
12 % walnut, there was no significant
difference compare to sham group.
Seminiferous diameters in control group
significantly decreased and in experimental
groups no significant difference in compared
to sham groups.
0.8
0.6
0.4
0.2
$
*
*
1.2
*
*
1
0.8
0.6
0.4
0.2
0
0
Sham
Control
Walnut 6% Walnut 9% Walnut 12%
Experimental groups
Sham
Control
Walnut 6%
Walnut 9%
Walnut 12%
Experimental groups
Figure 3. walnut effect on sex organs weight in diabetic adult male Rats
Congress name: 14th Congress on
Reproductive Biomedicine
Main field: male infertility