Synaptic Transmission • Classical – Mediated by Neurotransmitter Gated Ion Channel aka ionotropic receptors • Neuromodulatory – Mediated by Metabotropic Receptors Both cause a post-synaptic potential, ie a change in the Membrane potential of the post-synaptic plasma membrane The psp can be depolarizing or hyperpolarizing Synaptic Potentials and Their Integration • EPSP: excitatory post-synaptic potential • IPSP: inhibitory post-synaptic potential • Temporal Summation • Spatial Summation Classical Neurotransmission • Effects due to direct gating of ion channel • Direct postsynaptic effects last for tens of milliseconds • No secondary effects • Postsynaptic electrical effects are fast and strong Neuromuscular Junction • is always excitatory • is one for one • 1 AP in presynaptic MN= 1 AP in post-synaptic muscle NMJ caused by release of 200 synaptic vesicles • In the rest of the NS, it is not 1 for 1, the psp is so small that an AP is not always triggered at the hillock • AP can cause release of 1 synaptic vesicle Excitatory Transmission • Synaptic transmission that causes depolarization of the postsynaptic neuron • Increases the probability that the post synaptic neuron will fire an action potential • Increases amount of neurotransmitter released from post synaptic neuron by presynaptic facilitation Excitatory Post-synaptic Potential = EPSP • Depolarization of the post-synaptic membrane caused by the neurotransmitter brings the membrane potential close to the threshold for firing an action potential • Can increase sodium or calcium permeability or can be caused by decreasing potassium permeability Inhibitory Transmission • Synaptic transmission that causes transient hyperpolarization of the postsynaptic neuron • Decreases the probability that the post synaptic neuron will fire an action potential • This is called an inhibitory post-synaptic potential ipsp I.P.S.P. • Caused by increase in potassium permeability similar to the undershoot of the action potential • Increase in chloride permeability • If ECl=Vr then no change in Vr will be observed, however an epsp would be smaller if the Cl permeability is still high Neuronal Integration • Summing of all ipsp and epsp to determine if threshold has been met for AP generation • Based on temporal summation – Time constant • Based on spatial summation – Space constant Temporal Summation • Rapid firing from a single presynaptic input leads to repeated post-synaptic potentials in a short period of time • Causes repeated depolarization of membrane without time to go back to resting state • Allows a weak presynaptic input to generate an action potential in post synaptic neuron Time Constant • The amount of time that a psp will last at a given membrane location= tau • tau=membrane resistance x membrane capacitance • Time it takes for constant applied voltage to build up to 63% of its final value Temporal Summation • Neurons with membranes that have long time constants show more temporal summation for conduction of psp • Typical values are 10 msec • Membrane resistance is reflected by number of open channels and channel density Membrane Capacitance Spatial Summation • The simultaneous firing of multiple individual presynaptic neurons to one postsynaptic neuron. • The post-synaptic effects sum and can bring the post synaptic membrane closer or further away from threshold. Length constant • Distance that a psp can spread along the membrane= lambda • Lambda= resistance of membrane/resistance of cytoplasm • Distance along a neurite at which a constant applied voltage will decay to 37% of its original value. Common value is 100-300 um to mm. • The greater the membrane resistance, ie no channels the longer the psp travels Synaptic Integration • Look at Geometry of Inputs and the liklihood that any synapse will lead to an action potential in the axon of the postsynaptic neuron PreSynaptic Inhibition and Facilitation • Requires 3 synapses • The middle synapse can be active or inactive Types of CNS Synapses • • • • Axodendritic Axosomatic Axoaxonic Dendrodendritic Functional/Structural Synapse Classification • Gray’s Type I – Post-synaptic membrane is thicker than pre-synaptic – Asymmetrical – Excitatory • Gray’s Type II – Symmetrical synapse, pre & post-synaptic densities are similar thickness – Inhibitory