The GeneTACTM G3
Dr. Steve Hawkins
Technology Manager
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BioChip Technologies
PerkinElmer Life Sciences
Change the tool to change the function
Takes less
than 10
minutes
Gridding / replicating
Library management
Picking
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Micro-arraying
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BioChip Technologies
PerkinElmer Life Sciences
Simplicity
• A common user interface
• A standard method definition style
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BioChip Technologies
PerkinElmer Life Sciences
GeneTAC micro-arrayer G3
• GeneTAC G3 Library Management System
A multifunctional tool combining effective genomic library generation
and management with array production
– up to 4800spots (10 x 10 x 48) (equivalent to 76800 oligo spots)
– Spot size ~300 µm
– 32 or 48 solid pins
– Positional accuracy 100 µm
– Positional precision 10 µm
– 384 well plates
– multi-functional capabilities
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BioChip Technologies
PerkinElmer Life Sciences
The G3 uses a dip and print technique
for solid pins
GeneTAC G3
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BioChip Technologies
PerkinElmer Life Sciences
Fast cycle time
• Optimised motion for reproducible transfer of liquids
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BioChip Technologies
PerkinElmer Life Sciences
Printing BioChips
32 or 48 pin tool using solid Titanium pins
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BioChip Technologies
PerkinElmer Life Sciences
Marker genes are separate from the
library
cleaning
Marker plate holder
Slide holder
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BioChip Technologies
PerkinElmer Life Sciences
Example
Genes spotted: 1920
Probes: (1) Cy3 labeled cDNA probe
total
total
prepared from Human Adult
RNA
(2) Cy5 labeled cDNA probe
generated from Human Fetal
RNA
Marker: Q gene from Lambda
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BioChip Technologies
PerkinElmer Life Sciences
Effective Probe DNA Delivery
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BioChip Technologies
PerkinElmer Life Sciences
Tracability
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BioChip Technologies
PerkinElmer Life Sciences
GeneMAP™
Array
Chips
GeneMAP™
Mouse Clone
Array
12
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BioChip Technologies
PerkinElmer Life Sciences
Colony picking
rapid and accurate
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BioChip Technologies
PerkinElmer Life Sciences
Colony Picking
Can use 96 well, 384 well or agar plates
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BioChip Technologies
PerkinElmer Life Sciences
Colony Picking
Programmable number of ‘dips’
Clones arranged in known order and position
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BioChip Technologies
PerkinElmer Life Sciences
Compatible plate formats
• Input
• Output
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15cm Petri
10cm Petri
22cm Bio-Assay
10cm Bio-Assay
GS Culture dish
96 well plate
96 deep well
384 well plate
GS Culture dish
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BioChip Technologies
PerkinElmer Life Sciences
Plate selection
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BioChip Technologies
PerkinElmer Life Sciences
Plate selection
Using GS Culture
plates
Using Bioassay
input plates
Using Round Petri
dishes
Packard
18
BioChip Technologies
PerkinElmer Life Sciences
Sterilization
•Brushing to remove agar
•Sonication to sterilise
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BioChip Technologies
PerkinElmer Life Sciences
Sterilization
•Ethanol for final sterilisation
•Heat lamp to dry
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BioChip Technologies
PerkinElmer Life Sciences
Sterilization Protocol
define the time for each
step using arrows
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BioChip Technologies
PerkinElmer Life Sciences
Imaging
• On-board CCD Camera
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Optimised imaging
440,000 pixels on the chip
each pixel represents about 0.01 mm2
8 bit grey scale resolution
excellent blue / white discrimination
Packard
BioChip Technologies
PerkinElmer Life Sciences
Image processing - IP
The IP Settings window
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Example of the Grabbed Image
Edge detect
Size and shape
Circularity
Colour
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BioChip Technologies
PerkinElmer Life Sciences
Workflow
• Picking performance
– Typical capacity
• up to 20,000 clones per 8 hour day
• up to 15,000 clones per 8 hour day with double inoculation
– colonies between 0.5 and 4.0mm
– accuracy better than 99%
– select on basis of colour, size and shape
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BioChip Technologies
PerkinElmer Life Sciences
Gridding onto Hybond-N+
nitrocellulose filter
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BioChip Technologies
PerkinElmer Life Sciences
Keeping track
• custom patterns allow easy cross referencing of the hybridisation
results to the corresponding 384-well plate without the use of ink
gridding
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BioChip Technologies
PerkinElmer Life Sciences
Replication
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Direct copy of either 96 or 384 well plates using the gridding tool
Expand 1 x 384 well to 4 x 96
Contract 4 x 96 to 1 x384
Eliminate the effects of freeze-thaw cycles
Fast and accurate copying of libraries
M a s te r 1
M a s te r 5
C op y 1a
C op y 3a
M a s te r 2
M a s te r 6
C op y 1b
C op y 3b
M a s te r 3
M a s te r 7
C op y 2a
C op y 4a
M a s te r 4
M a s te r 8
C op y 2b
C op y 4b
C op y 5a
C op y 7a
C op y 5b
C op y 7b
C op y 6a
C op y 8a
C op y 6b
C op y 8b
S e c o n d T ra y
U s e r p rom p te d
w h e n to c h a n g e
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BioChip Technologies
PerkinElmer Life Sciences
Replication
Library management
Selectively re-array individual
elements from a library
Addresses individual wells in any
library plate
Rapid set-up via
standard spreadsheet
Full report generated
Optional autoloader for
total plate management
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Packard
BioChip Technologies
PerkinElmer Life Sciences