Diptera (True Flies) Veterinary Entomology 208 Fall 2010 Fly taxonomy Traditional classification: Brachycera (‘short horn’) • fat-bodied flies • short antennae • Examples: horse flies, house flies, etc. Nematocera (‘thread horn’) • slender-bodied flies • long, slender antennae • Examples: gnats, mosquitoes, crane flies Brachycera Adults: taxa to know in key • • • • • • • • Hippoboscidae (keds) Tabanidae (horse flies, deer flies) ‘horse fly’ ‘deer fly’ Calliphoridae Cochliomyia sp. ‘calliphorid spp.’ Muscidae Stomoxys calcitrans (stable fly) Haematobia irritans (horn fly) Musca domestica (house fly) Musca autumnalis (face fly) Key characters and taxa Hippoboscidae • wings absent • tick-like in appearance www.dkimages.com Key characters and taxa Antennae • aristate • stylate (Tabanidae) Tabanidae horse fly deer fly (no specimens on exam) Key characters and taxa spiracle bristle row hypopleuron Hypopleural bristles • present (Calliphoridae) • absent (Muscidae) Calliphoridae Cochliomyia sp. calliphorid spp. Key characters and taxa Mouthparts • piercing • sponging (Musca) palps palps short palps long Stomoxys calcitrans Haematobia irritans parafrontal area intermediately wide very wide or very narrow Musca domestica Musca autumnalis Brachycera Larvae: taxa to know in key • • • • • • • • Oestridae (botflies, cattle grubs) Gasterophilus sp. (horse bots) Hypoderma sp. (cattle grubs) Calliphoridae (blowflies, screwworms) Cochliomyia hominivorax (primary screwworm) ‘calliphorid spp.’ (blowflies) Muscidae (house fly, stable fly, horn fly, face fly) Stomoxys calcitrans (stable fly) Haematobia irritans (horn fly) Musca domestica (house fly) Musca autumnalis (face fly) larvae shape small and slender large and robust other Brachycera Oestridae Oestridae heavy spines Gasterophilus sp. spines small or absent Hypoderma sp. slits of posterior spiracles straight sinuous (squiggly) Calliphoridae Muscidae Calliphoridae Posterior tracheal trunks pigmented, visible Cochliomyia hominivorax Posterior tracheal trunks unpigmented, not visible calliphorid sp. Muscidae spiracles widely separated, button centrally located Stomoxys calcitrans spiracles proximate, button laterally located other Muscidae Muscidae spiracle with outer margin dark, ring-like entire spiracle dark, without well-defined, dark margin Musca domestica other Muscidae Muscidae 15 mm 7 mm Musca autumnalis Haematobia irritans Culicidae adults Taxa to know in key to adult females Aedes Anopheles Culex Culiseta Psorophora Toxorhynchites palp length as long as proboscis short Anopheles other Culicidae proboscis shape slender and curved ventrad apically same diameter throughout, straight Toxorhynchites other Culicidae bristles spiracular bristles present, post-spiracular bristles present spiracular bristles present, post spiracular bristles absent Psorophora Culiseta bristles spiracular bristles absent, postspiracular bristles present spiracular bristles absent, postspiracular bristles absent Aedes Culex bristles spiracular bristles post-spiracular bristles Psorophora + + Culiseta + – Aedes – + Culex – – Culicidae larvae Taxa to know in key to larvae Aedes Anopheles Culex Culiseta Psorophora air tube absent present Anopheles other Culicidae air tube pecten • comb-like arrangement of setae • positioned near base of tube • all species included in lab have pecten air tube setal tufts basal tuft absent, other tufts present basal tuft present, other tufts absent Culex Culesita anal segment plate complete as a ring around segment plate not complete as a ring around segment, forming ‘saddle’ Psorophora Aedes