Foss Winescan vs. OenoFoss: Capabilities, Cost and Tradeoffs Paul Huckaba Analytical Laboratory Manager Bronco Wine Company What is Spectroscopy? How does it work? What types of instruments are there? What analyses can these instruments run? What are the Pros and Cons? How do you run samples? Who is this best suited for? What kind of results can I expect? Spectroscopy is the study of the interaction between matter and radiated energy (“light”). There are lots of kinds of light energy. Spectroscopy can be used to analyze wine or must samples. There are no chemicals or consumables AND the analysis only takes 1-2 minutes! Infrared (IR)-laser The IR laser source generates IR light through the sample, at a specific wavelength range Result of the measured sample is presented to the user. ”Clean” Sample The IR light passing through the sample is absorbed by different molecules in the sample. The remaining light passes through the sample and is collected in a series of IR interferograms Single Beam Spectrum In the PC, the spectrum goes through a series of calculations against the calibration models in order to derive the results (3,000 sample wine library) Images Courtesy of Gusmer Enterprises IR-Interferogram The interferograms are sent to the PC where FTIR mathematics transforms the interferograms into single beam spectra Currently, there are two main instruments. Foss Winescan (FT120, Flex, etc.) OenoFoss Both are made by Foss, a Danish Company The capabilities depend on the type of instrument that you have, the matrix (must, wine, etc.), and what you have calibrated for, but here are the highlights… Ethanol Glucose+Fructose Malic Acid TA (Total Acid) pH Volatile Acidity NOPA/YAN + Others… Tartaric Acid to pH=8.2 Pros -Easy to use -No chemicals -Very quick Cons -Requires Calibration -Cost -Data may not be “absolutely” accurate OenoFoss OenoFoss OenoFoss Foss Winescan Foss Winescan Foss Winescan Larger footprint Needs periodic maintenance Takes about a minute for duplicate analysis Can be Autosampled Can run more tests Has available SO2 modules (on Flex model) OenoFoss Smaller footprint No moving parts, so no real maintenance Takes about 2 minutes for duplicate analysis Less Expensive Can’t automate Foss Winescan Best For Medium-to-Large sized winery labs that need more throughput and can devote resources to calibration Laboratories needing Autosampler Capability OenoFoss Best For Smaller sized winery labs that need quick answers Labs with small sample loads Labs with nontechnical staff We ran a study of these two instruments, comparing them to our “Wet Chemistry” methods. Red and White wines were tested. We have calibrated our Winescan, but the OenoFoss used a “factory” calibration. This is a comparison of our results, but as always…Your mileage may vary. “Delta values” Wine 001MOU 960PTS 061GRN 901PNR 801DDR 960NMR 009PNR 009PNR 002CSV 060SHZ 936MER 050PNR Average Alc 0.23 0.16 0.08 0.19 0.19 0.17 0.15 0.18 0.10 0.09 0.09 0.24 0.16 FT 120 Red Wines TA -0.01 0.19 -0.20 0.06 0.16 0.00 0.04 -0.01 0.05 -0.08 0.17 0.07 0.09 pH -0.06 0.02 -0.07 -0.02 0.00 -0.01 0.00 -0.01 -0.02 0.01 0.00 -0.01 0.02 G+F 0.92 0.10 0.16 0.14 -0.06 0.44 0.34 0.30 0.39 0.19 0.17 0.00 0.27 VA 0.060 0.045 0.141 0.097 0.091 0.113 -0.009 -0.015 0.005 0.094 0.011 0.062 0.062 “Delta values” Wine 001SYM 970LSB 901LCD 802LSB 972LCD 008MOS 060SCC 002FRC 928CHD 001DWH 960RCD 905CHD Average Alc 0.12 0.34 0.29 0.28 0.32 0.32 0.32 0.23 0.26 0.18 0.25 0.17 0.26 FT 120 White Wines TA 0.09 0.12 0.68 0.50 0.19 0.16 0.22 0.46 0.50 -0.03 0.52 0.53 0.33 pH 0.09 -0.08 -0.01 -0.06 -0.02 0.04 0.02 -0.02 -0.01 -0.04 -0.04 0.00 0.04 G+F N/A -0.09 -0.69 -0.57 -0.23 N/A -0.33 -0.10 -0.30 -0.23 -0.37 -0.67 0.36 VA 0.071 0.028 -0.065 -0.021 -0.036 0.104 -0.007 0.006 -0.023 -0.095 -0.005 -0.079 0.045 “Delta values” Wine 001MOU 960PTS 061GRN 901PNR 801DDR 960NMR 009PNR 009PNR 002CSV 060SHZ 936MER 050PNR Average Alc -0.13 -1.22 0.01 0.07 -0.09 -0.93 -0.77 -0.10 0.09 -0.07 -0.10 -0.78 0.36 OenoFoss Red Wines TA -0.20 0.10 -0.20 -0.40 -0.40 -0.05 -0.10 -0.45 -0.20 -0.45 -0.10 0.05 0.23 pH -0.05 0.07 -0.04 0.01 0.04 0.11 0.00 -0.03 0.19 -0.02 0.15 -0.08 0.07 G+F -0.26 -0.49 -0.01 -0.67 -2.80 -0.17 -0.61 -0.64 -0.17 -0.02 -0.52 -0.42 0.57 VA -0.020 0.090 0.170 0.120 -0.030 0.150 -0.005 -0.020 -0.030 0.005 -0.040 0.080 0.063 “Delta values” Wine 001SYM 970LSB 901LCD 802LSB 972LCD 008MOS 060SCC 002FRC 928CHD 001DWH 960RCD 905CHD Average Alc N/A -0.70 -0.12 0.01 -1.17 0.45 -0.80 0.01 -1.59 -0.57 0.04 -0.72 0.56 OenoFoss White Wines TA 0.30 0.05 0.10 0.05 0.05 0.40 -0.25 0.05 0.05 -0.40 0.05 0.10 0.15 pH 0.47 -0.02 0.06 -0.09 0.07 0.17 0.12 0.03 0.07 0.02 0.03 0.13 0.11 G+F N/A -0.14 -0.70 -0.57 -1.02 N/A -0.26 -0.37 -0.53 -1.17 -0.37 -0.67 0.58 VA 0.110 0.090 -0.005 0.030 -0.070 0.190 0.100 0.100 0.000 0.060 0.140 -0.100 0.083 The data from the 2011 trial was basically just using the “off the shelf” calibration. We wanted to see if we would see the same sort of discrepancies, and if so, could we could do better by calibrating. All Infrared instruments tend to better when calibrated with the sample types being tested. Oeno Foss Variety EtOH G/F Malic pH TA VA PNG 12.74 6.92 1.745 3.34 5.77 0.169 12.59 7.78 - 3.35 5.54 0.233 13.75 0.00 3.055 3.47 6.38 0.152 13.47 0.52 3.787 3.48 6.44 0.267 13.50 5.07 0.879 3.39 5.98 0.308 13.52 6.19 - 3.40 5.74 0.315 12.29 6.40 2.545 3.34 6.40 0.024 12.43 7.99 - 3.39 6.32 0.156 13.50 0.00 3.268 3.49 6.57 0.268 13.41 0.52 3.787 3.49 6.37 0.257 10.30 21.09 2.901 3.21 6.60 0.188 10.05 23.65 - 3.32 6.11 0.177 Historical Oeno Foss LCD Historical Oeno Foss CHD Historical Oeno Foss PNG Historical Oeno Foss LCD Historical Oeno Foss Historical RSL Oeno Foss Variety EtOH G/F Malic pH TA VA CHD 13.48 0.00 2.635 3.71 5.23 0.163 13.37 0.21 2.760 3.83 4.72 0.128 12.56 0.03 2.794 3.56 5.94 0.238 12.48 0.97 - 3.68 5.92 0.220 13.53 3.22 2.301 3.43 6.37 0.298 13.48 4.34 2.480 3.39 6.05 0.286 12.82 23.06 1.662 3.35 5.62 0.251 12.58 24.53 - 3.29 5.37 0.212 12.61 1.09 2.103 3.67 5.29 0.006 12.60 1.67 - 3.79 5.17 0.098 13.62 0.00 2.546 3.39 6.03 0.243 13.57 0.61 - 3.41 5.65 0.206 Historical Oeno Foss LSB Historical Oeno Foss CSB Historical Oeno Foss GWT Historical Oeno Foss WPG Historical Oeno Foss Historical CHD Oeno Foss Variety EtOH G/F Malic pH TA VA CHD 13.24 0.00 2.489 3.39 5.94 0.155 13.30 0.27 2.547 3.45 5.70 0.130 13.19 0.34 1.599 3.36 5.50 0.093 13.34 0.40 1.637 3.39 5.03 0.071 13.52 0.15 1.474 3.54 4.81 0.062 13.57 0.50 1.507 3.62 4.35 0.083 13.72 0.00 2.808 3.59 5.75 0.202 13.56 0.44 - 3.79 4.76 0.219 13.26 0.00 3.013 3.45 6.61 0.207 13.24 0.47 3.078 3.50 6.32 0.158 13.17 0.00 2.887 3.70 5.31 0.154 13.07 0.52 3.149 3.84 4.81 0.113 Historical Oeno Foss LPG Historical Oeno Foss PNG Historical Oeno Foss CHD Historical Oeno Foss CHD Historical Oeno Foss Historical CHD Oeno Foss Variety EtOH G/F Malic pH TA VA CHD 13.14 0.00 0.292 3.47 5.73 0.232 13.39 0.29 0.205 3.45 5.92 0.242 12.94 3.15 2.301 3.35 6.14 0.226 12.86 3.94 - 3.38 5.77 0.195 13.76 0.13 1.717 3.49 5.56 0.127 13.83 0.31 0.302 3.59 5.19 0.155 13.11 0.47 2.087 3.37 6.02 0.184 13.03 0.23 2.326 3.39 5.60 0.166 13.43 0.54 1.502 3.36 5.44 0.089 13.34 0.40 1.637 3.39 5.03 0.129 13.43 0.00 1.959 3.51 6.46 0.408 13.60 0.34 - 3.50 6.31 0.358 Historical Oeno Foss DWH Historical Oeno Foss LPG Historical Oeno Foss PNG Historical Oeno Foss LPG Historical Oeno Foss Historical CSB Oeno Foss Variety EtOH G/F Malic pH TA VA LSB 13.66 0.61 2.250 3.29 6.11 0.209 13.74 0.97 2.629 3.35 5.90 0.229 13.56 0.33 1.850 3.32 5.80 0.103 13.52 0.48 1.582 3.30 5.34 0.092 13.55 0.00 2.581 3.41 6.01 0.221 13.43 0.50 - 3.42 5.80 0.164 13.44 0.27 2.469 3.30 6.16 0.167 13.51 0.60 - 3.34 6.00 0.194 13.20 0.00 2.484 3.32 6.13 0.038 13.01 0.80 - 3.49 5.76 0.100 13.28 0.00 2.729 3.38 6.01 0.184 13.29 0.24 2.932 3.39 5.79 0.184 Historical Oeno Foss LPG Historical Oeno Foss CHD Historical Oeno Foss LSB Historical Oeno Foss WPG Historical Oeno Foss Historical CHD Oeno Foss Variety EtOH G/F Malic pH TA VA WPG 12.50 0.64 2.165 3.32 5.75 0.181 12.42 0.79 - 3.38 5.78 0.211 13.46 4.63 1.217 3.39 6.02 0.234 13.34 6.45 - 3.40 5.95 0.300 10.38 0.00 4.405 3.39 7.37 0.114 10.22 0.05 4.881 3.45 7.00 0.100 14.07 0.00 0.231 3.40 5.49 0.330 14.07 0.32 - 3.33 5.13 0.234 13.02 0.13 2.146 3.64 5.22 0.068 12.81 0.38 2.896 3.81 4.78 0.122 13.66 0.56 0.929 3.47 4.78 0.133 13.33 0.42 1.113 3.35 5.79 0.143 Historical Oeno Foss CHD Historical Oeno Foss CHD Historical Oeno Foss CHD Historical Oeno Foss PNG Historical Oeno Foss Historical PNG “Delta values” Variety EtOH G/F Malic pH TA VA PNG -0.15 0.86 - 0.01 -0.23 0.064 LCD -0.28 0.52 0.73 0.01 0.06 0.115 CHD 0.02 1.12 - 0.01 -0.24 0.007 PNG 0.14 1.59 - 0.05 -0.08 0.132 LCD -0.09 0.52 0.52 0.00 -0.20 -0.011 RSL -0.25 2.56 - 0.11 -0.49 -0.011 Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical EtOH G/F Yellow 0.15 Red 0.25 Malic pH TA VA 1.00 0.05 0.30 0.030 1.50 0.10 0.50 0.050 “Delta values” Variety EtOH G/F Malic pH TA VA CHD -0.11 0.21 0.13 0.12 -0.51 -0.035 LSB -0.08 0.94 - 0.12 -0.02 -0.018 CSB -0.05 1.12 0.18 -0.04 -0.32 -0.012 GWT -0.24 1.47 - -0.06 -0.25 -0.039 WPG -0.01 0.58 - 0.12 -0.12 0.092 CHD -0.05 0.61 - 0.02 -0.38 -0.037 Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical EtOH G/F Yellow 0.15 Red 0.25 Malic pH TA VA 1.00 0.05 0.30 0.030 1.50 0.10 0.50 0.050 “Delta values” Variety EtOH G/F Malic pH TA VA CHD 0.06 0.27 0.06 0.06 -0.24 -0.025 LPG 0.15 0.06 0.04 0.03 -0.47 -0.022 PNG 0.05 0.35 0.03 0.08 -0.46 0.021 CHD -0.16 0.44 - 0.20 -0.99 0.017 CHD -0.02 0.47 0.06 0.05 -0.29 -0.049 CHD -0.10 0.52 0.26 0.14 -0.50 -0.041 Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical EtOH G/F Yellow 0.15 Red 0.25 Malic pH TA VA 1.00 0.05 0.30 0.030 1.50 0.10 0.50 0.050 “Delta values” Variety EtOH G/F Malic pH TA VA CHD 0.25 0.29 -0.09 -0.02 0.19 0.010 DWH -0.08 0.79 - 0.03 -0.37 -0.031 LPG 0.07 0.18 -1.42 0.10 -0.37 0.028 PNG -0.08 -0.24 0.24 0.02 -0.42 -0.018 LPG -0.09 -0.14 0.14 0.03 -0.41 0.040 CSB 0.17 0.34 - -0.01 -0.15 -0.050 Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical EtOH G/F Yellow 0.15 Red 0.25 Malic pH TA VA 1.00 0.05 0.30 0.030 1.50 0.10 0.50 0.050 “Delta values” Variety EtOH G/F Malic pH TA VA LSB 0.08 0.36 0.38 0.06 -0.21 0.020 LPG -0.04 0.15 -0.27 -0.02 -0.46 -0.011 CHD -0.12 0.50 - 0.01 -0.21 -0.057 LSB 0.07 0.33 - 0.04 -0.16 0.027 WPG -0.19 0.80 - 0.17 -0.37 0.062 CHD 0.01 0.24 0.20 0.01 -0.22 0.000 Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical EtOH G/F Yellow 0.15 Red 0.25 Malic pH TA VA 1.00 0.05 0.30 0.030 1.50 0.10 0.50 0.050 “Delta values” Variety EtOH G/F Malic pH TA VA WPG -0.08 0.15 - 0.06 0.03 0.030 CHD -0.12 1.82 - 0.01 -0.07 0.066 CHD -0.16 0.05 0.48 0.06 -0.37 -0.014 CHD 0.00 0.32 - -0.07 -0.36 -0.096 PNG -0.21 0.25 0.75 0.17 -0.44 0.054 PNG -0.33 -0.14 0.18 -0.12 1.01 0.010 Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical Oeno Foss Historical EtOH G/F Yellow 0.15 Red 0.25 Malic pH TA VA 1.00 0.05 0.30 0.030 1.50 0.10 0.50 0.050 We wanted to see if we could improve upon the data that we generated if we calibrated the OenoFoss with our data. Data from 36 samples were added into the calibration. 12 samples were then run to evaluate the effect of the recalibration. So…what did we see? “Delta Values” Variety EtOH G/F Malic pH TA VA Pre-calibration LCD 0.28 0.52 0.73 0.01 0.06 0.115 Post calibration LCD 0.02 0.14 0.17 0.01 0.06 0.021 Pre-calibration CHD 0.02 1.12 - 0.01 0.24 0.007 Post calibration CHD 0.05 0.05 - 0.02 0.07 0.077 Pre-calibration PNG 0.14 1.59 - 0.05 0.08 0.132 Post calibration PNG 0.03 0.24 - 0.07 0.11 0.087 Pre-calibration LCD 0.09 0.52 0.52 0.00 0.20 0.011 Post calibration LCD 0.08 0.17 0.07 0.01 0.24 0.022 >0.1 >1.0 >0.4 >0.05 >0.2 >0.03 “Delta Values” Variety EtOH G/F Malic pH TA VA Pre-calibration RSL 0.25 2.56 - 0.11 0.49 0.011 Post calibration RSL 0.00 0.33 - 0.17 0.34 0.008 Pre-calibration CHD 0.11 0.21 0.13 0.12 0.51 0.035 Post calibration CHD 0.03 0.14 0.09 0.08 0.15 0.034 Pre-calibration LSB 0.08 0.94 - 0.12 0.02 0.018 Post calibration LSB 0.16 0.07 - 0.09 0.17 0.001 Pre-calibration CSB 0.05 1.12 0.18 0.04 0.32 0.012 Post calibration CBS 0.00 0.17 0.09 0.02 0.08 0.030 >0.1 >1.0 >0.4 >0.05 >0.2 >0.03 “Delta Values” Variety EtOH G/F Malic pH TA VA Pre-calibration GWT 0.24 1.47 - 0.06 0.25 0.039 Post calibration GWT 0.02 1023.53* - 0.03 0.06 0.008 (*too sweet) Pre-calibration WPG 0.01 0.58 - 0.12 0.12 0.092 Post calibration WPG 0.09 0.06 - 0.05 0.14 0.065 Pre-calibration CHD 0.05 0.61 - 0.02 0.38 0.037 Post calibration CHD 0.06 0.06 - 0.04 0.13 0.022 >0.1 >1.0 >0.4 >0.05 >0.2 >0.03 Overall, the data were better. We went from 30 outliers (out of 59 test results) to 11 outliers. Only 2 results went from what we would consider “acceptable” to “not acceptable”. Most results got quite a bit closer to the Wet Chemistry values. Variety PNG EtOH 12.74 12.59 G/F 6.92 7.78 Malic 1.745 - pH 3.34 3.35 TA 5.77 5.54 VA 0.169 0.233 Oeno Foss Historical LCD 13.75 13.47 0.00 0.52 3.055 3.787 3.47 3.48 6.38 6.44 0.152 0.267 Oeno Foss Historical CHD 13.50 13.52 5.07 6.19 0.879 - 3.39 3.40 5.98 5.74 0.308 0.315 Oeno Foss Historical PNG 12.29 12.43 6.40 7.99 2.545 - 3.34 3.39 6.40 6.32 0.024 0.156 Oeno Foss Historical LCD 13.50 13.41 0.00 0.52 3.268 3.787 3.49 3.49 6.57 6.37 0.268 0.257 Oeno Foss Historical RSL 10.30 10.05 21.09 23.65 2.901 - 3.21 3.32 6.60 6.11 0.188 0.177 Oeno Foss Historical If desired, you can have different profiles for red, white, dry, sweet, etc. Units & significant digits can be customized. Searchable history by lot number. Reports can be generated by date, tank, lot number, etc. and exported to Excel. Can also export to LIMS, SQL, CSV, XML formats. is just starting out and has no lab equipment has little manpower in the lab, or a few people who “wear multiple hats”. has multiple (small) locations and wants to minimize variation between locations. is struggling to find qualified lab techs. is wanting to use NIR technology, but just can’t justify a Foss Winescan. Calibrations are available for a number of analytes, but they can be expensive ($5K-$10K), or you can do them yourself. For the OenoFoss, the instrument is limited to one-at-a-time analysis, and has a more limited Alcohol range (8-18%) and Glucose+Fructose range (0-25 g/L). Neither instrument likes chunky, gassy samples (can degas/centrifuge). Spectroscopic techniques are very fast, inexpensive to run, and especially good for looking for patterns/trends, especially if you are running the same wines. It will take some effort on your part to get the most out of your instrument. If these instruments fit your winery needs, they can be a valuable tool. Thank you.