Crayfish in Scotland Zara Gladman z.gladman.1@research.gla.ac.uk Overview Crayfish in Scotland North American signal crayfish White-clawed crayfish Research so far: a synopsis What next? Crayfish in Scotland Crayfish are naturally absent from Scotland Two introduced species: White-clawed crayfish (Austropotamobius pallipes) North American signal crayfish (Pacifastacus leniusculus) Crayfish in Scotland Signal crayfish (Pacifastacus leniusculus) First recorded in the wild in Galloway, southern Scotland in 1995 Now inhabits ~ 174 km river length (13 catchments) and several standing waters Serious threat to biodiversity – pressing need for research ‘Species Action Framework’, 2007 Crayfish in Scotland White-clawed crayfish (Austropotamobius pallipes) Native to Europe, including England, Wales and Ireland – but not Scotland Two Scottish populations Non-native but protected species (Schedule 5 of Wildlife & Countryside Act 1981, Appendix III of Bern Convention, Annexes IIa and Va of EC Habitats Directive and the Nature Conservation (Scotland) Act 2004, IUCN Red List of Threatened Species) Research so far . . . 1. White-clawed crayfish Status of Scottish populations 2. Signal crayfish Distribution Movements (radiotracking) Control (trapping at Loch Ken) Impact 1. White-clawed crayfish Two introduced populations Durness (Thomas, 1992) Renfrewshire (Maitland, 2001) Aim: survey both sites to assess the status of these populations Surveys Trapping Hand-searching Night viewing Electrofishing Invertebrate sampling Surveys Providing data on: Distribution Population structure Relative density Stable isotope samples Research so far . . . 1. White-clawed crayfish Status of Scottish populations 2. Signal crayfish Distribution Movements Control Impact 2. Signal crayfish Distribution Methods for detecting signal crayfish in riffles – comparative field study Gladman et al. (2010), Aquatic Conserv: Mar. Freshw. Ecosyst. 20: 588-594 2. Signal crayfish Movements Radio-tracking, mark-and-recapture studies 2. Signal crayfish Control Effects of trapping on crayfish population at Loch Ken, southern Scotland 2. Signal crayfish Control 2. Signal crayfish Control Summer 2009: Marine Scotland funded an intensive 4-month trapping programme: Assess the scale of the infestation Assess the feasibility of control Opportunity for research: Effect of intensive trapping on the population Two mark-and-recapture projects: one before and one after the trapping programme 2. Signal crayfish Control Crayfish sampled at 3 sites (transects of 15 creels, 400 m apart) during two sessions: before (May/June) and after (September) the trapping programme 2. Signal crayfish Control Summary Trapping significantly reduced males; effect on females complicated by trap bias/reproductive status sex ratio increased towards females Mean size of crayfish reduced Crayfish capable of significant movements High densities mean loss to biodiversity likely to be significant 2. Signal crayfish Impact Signal crayfish: a threat to Atlantic salmon? Signal crayfish: a threat to Atlantic salmon? Atlantic salmon in Scotland Valued part of natural heritage Significant economic importance (salmon and sea trout: £73 million, annually) Conservation of high priority Salmon Conservation (Scotland) Act, 2001 EU Habitats Directive, 1992 Signal crayfish: a threat to Atlantic salmon? Previous research Reduced recruitment of salmonid fish in English stream (Peay et al., 2009) No impact on recruitment (Degerman et al., 2007) Circumstances/mechanism(s) behind declines unclear Signal crayfish outcompete juvenile Atlantic salmon for shelter (Griffiths et al., 2004) Interactions during other stages of development? Egg predation? Salmonid eggs are palatable to signal crayfish (Nyberg & Degerman, 2000) Eggs buried in redds? Signal crayfish: a threat to Atlantic salmon? Aim: To investigate the ability of signal crayfish to detect and excavate artificial salmonid redds Signal crayfish: a threat to Atlantic salmon? Preparation: Indoor stream fed with water from the River Almond Stream divided into 16 test arenas Each arena filled with gravel + two pieces of plastic tubing Impact Ability of crayfish to detect and excavate boxes containing: Signal crayfish: a threat to Atlantic salmon? Atlantic salmon eggs Fish (Clupea herengus L.) Nothing (control) Boxes buried 5 cm below gravel surface One adult male signal crayfish added to each arena Signal crayfish: a threat to Atlantic salmon? Signal crayfish: a threat to Atlantic salmon? Three successive trials: Treatment 1 (eggs) Treatment 2 (fish) Treatment 3 (eggs) (+ empty ‘control’ boxes) Signal crayfish: a threat to Atlantic salmon? Observations: Number/location/dimensions of excavations recorded daily At the end of each treatment, number of arenas containing: No excavations Minor excavations (≤ 2 cm depth) Major excavations (> 2 cm depth) Complete excavations (box exposed) . . . in box area (within 5 cm radius) recorded Results: Empty ‘control’ boxes undisturbed Treatment 1 (eggs) After 14 nights . . . . Treatment 2 (fish) After one night . . . 3 arenas with major excavations, 3 arenas with complete excavations After two nights . . . 1 arena with a minor excavation, 11 arenas with no excavations Existing excavations deepened, 4 arenas with new excavations 5 arenas with complete excavations, 5 arenas with major excavations Treatment 3 (eggs) After seven nights . . . No excavations in any arena Treatment 1 (eggs) Treatment 2 (herring) Treatment 3 (eggs) 12.00 Number of Arenas 10.00 8.00 6.00 4.00 2.00 0.00 None Major Minor None Complete Major Minor None Complete Major Minor Complete Excavations Number of arenas containing excavations in the box area is significantly linked to bait type (X2Yates = 20.05, p<0.001 ) Signal crayfish: a threat to Atlantic salmon? Conclusions: Signal crayfish could not detect salmon eggs buried at this depth low risk of predation in the wild? Vulnerability of redds likely vary according to a range of biotic and abiotic factors: Egg – stage of development, dead/damaged eggs, density, burial depth; risk to newly hatched alevins? Crayfish – density, age Gravel size – burrowing ability/spawn site selection Impact of egg predation on recruitment should be assessed on a case-by-case basis What next? Impact of signal crayfish on native biodiversity: Freshwater pearl mussels (currently) Stable isotopes analysis: white-clawed crayfish samples . . . writing up! Thanks to . . . Colin Adams Colin Bean Jo Long Willie Yeomans z.gladman.1@research.gla.ac.uk