TisXell
Regeneration System
Company Background
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 QuinXell Technologies Pte Ltd was founded in 2011
 QuinXell is the latest addition to Quintech Life Sciences
(QLS) family of companies established to tap into the
emerging Life Science industry
 QuinXell will focus in the area of Regenerative Medicine
through its innovative cell culture-based technologies
Company Background
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 Research on QuinXell’s TisXell Regeneration System
was initiated as a joint project between the National
University of Singapore (NUS) and Singapore Polytechnic
(SP), with the National University Hospital (NUH) providing
samples for applications
 Scientific
papers supporting the benefits of biaxial
bioreactor on functional tissue growth (tissue engineered
constructs or TECs) on 3D scaffolds have been published in
international journals for more than 10 years
Background Information
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 Regenerative Medicine has been hailed as a revolutionary
medical advancement, breathing new life into patient care
 Transplantation through in vitro cultures of organs and tissues
or Tissue Engineering (TE) is a standardized and
reproducible method to create functional tissues in the
laboratory
 These
tissues are utilized to replace, repair or
regenerate organs loss due to disease, trauma, failure or
cogenital defects
Background Information
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 Traditionally, spinner flasks, rotating wall vessels (RWV),
perfusion and uniaxial bioreactors are employed for TE
 QuinXell’s unique TisXell Regeneration System offers
an optimal condition for high growth and high proliferation of
cell cultures compared to conventional methods.
 TisXell controlled environment provides optimal nutrients
and gases to growing cells and triggers cellular mechanotransduction signalling pathways to stimulate tissue
remodelling onto a 3D scaffold.
Application Fields
For research institutes, hospitals, biotechnology, pharma in
• Regenerative medicine
• Tissue engineering
• Translational medicine
• Tissue and organ transplant
Cell Types
• Primary Cells
• Stem Cells
• Bone Cells
• Muscles Cells
• And many more…
Conventional Methods
Types of Bioreactors
Advantages
Spinner flasks
Improved fluid flow (as compared Turbulence generated can be
to static vessels)
detrimental for seeded cells and
newly laid down ECM
Perfusion bioreactors
Enhances mesenchymal stem cell
proliferation and osteogenic
differentiation in scaffold
constructs
Rotating wall vessel (RWV)
bioreactors
Disadvantages
Non-homgenous cellular
distribution
Cells at frontal zones are washed
away by the oncoming perfusion
flow with higher flow rates
Low sheer forces and 3D high
Non-homogenous cellular
mass transfer (similar to perfusion distribution and ECM deposition
bioreactors)
In the free floating culture,
collision between scaffolds and
bioreactor walls may induce
cellular damage and disrupt
cellular attachment and matrix
deposition on the scaffolds
TisXell Novel Approach
Types of Bioreactors
Biaxial revolving bioreactor
e.g. TisXell
Advantages
Integrated perfusion system allows for exchange
between the vessel and reservoir with maximal
mass transfer under low sheer force
Biaxial revolution of the culture vessel led to more
homogenous cellular and ECM deposition – at the
surface and the deeper core of the scaffolds
Cellular scaffolds were secured by pins to avoid
scaffold and vessel wall collisions
TisXell Regeneration System
TisXell – Unique Selling Points
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 Patented biaxial revolution in two independent orthogonal
axes (spin and tumble simultaneously)
 Flexible operational modes available – biaxial, uniaxial, swing
modes
TisXell – Unique Selling Points
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 Efficient fluid transport within the 3D scaffolds, allowing
optimal nutrients and waste exchange to and from the cells,
penetrating the deepest core of the scaffolds
 Spherical design of vessel reduces drag and promotes
uniform fluid mixing
TisXell – Key Benefits
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 Accelerates cell growth, differentiation and cell proliferation,
mimicking native extracellular matrix (ECM)
 Supports homogenous cell culture at the surface and core of
the 3D scaffolds
 Maintains functionality and viability of tissue constructs
TisXell – Key Benefits
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 Reduces formation of necrotic neotissue that interferes with
subsequent implantation process
 Promotes integration of implants with surrounding tissue and
support the structural integrity in regeneration of tissues
and/or organs
Key Benefits for Bone Applications
InVitro
 Homogenous cellular distribution
 Rapid stem cell expansion – up to 2-fold (or more)
improvement in mass transfer in thicker grafts
 High viability
 Robust osteogenic differentiation and mineralization
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Key Benefits for Bone Applications
InVivo
 Increased bone formation after transplantation
 Promotes new vascularization
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TisXell System Configuration I
TisXell Regeneration System, Basic
Includes
o Two spherical glass vessels of 500ml and 1000ml, 1 each
o Revolving stand to hold one vessel, 1
o Integrated peristaltic pump, 1
o Medium reservoir, 1
o Remote touch screen panel, 1
o Tubings and connectors starter kit, 1
o Scaffolds starter kit, 1
TisXell System Configuration II
TisXell Regeneration System, Complete
Includes
o Two spherical glass vessels of 500ml and 1000ml, 1 each
o Revolving stand to hold one vessel, 1
o Integrated peristaltic pump, 1
o Medium reservoir, 1
o Remote touch screen panel, 1
o Tubings and connectors starter kit, 1
o Scaffolds starter kit, 1
o Set of probes, 1
o Applikon console and monitor, 1