AP LAB #3 part C Crossing Over during Meiosis in Sordaria… counting map units Sordaria fimicola is a type of FUNGUS, found in the group known as ASCOMYCOTA “Sac fungi” The shared derived character that defines the Ascomycota is the ascus. It is within the ascus that nuclear fusion and meiosis take place. Ascus are found in here… in the ascocarp! Sordaria fimicola~ ASCOMYCOTA See, here they are… ASCI! Sordaria fimicola~ ASCOMYCOTA This is what it looks under a scanning emicroscope! Sordaria fimicola~ ASCOMYCOTA Here they are… ASCI! ~ they hold ASCOSPORE Sordaria fimicola~ ASCOMYCOTA Here they are… ASCI! ~ there are chromosomes inside those dots!! In the ascus, meiosis occurs, followed by a round of mitosis. This leaves eight nuclei, and eventually eight ascospores THERE ARE CHROMOSOMES INSIDE THOSE SPORES… AND WITHIN THE CHROMATIN, THERE ARE GENES…. These genes code for traits… COLOR Ascospores are formed within the ascus by an enveloping membrane system, which packages each nucleus with its adjacent cytoplasm and provides the site for ascospore wall formation Sordaria fimicola~ ASCOMYCOTA Sordaria fimicola~ ASCOMYCOTA S phase Meiosis I Meiosis II Sordaria fimicola~ ASCOMYCOTA Sordaria fimicola~ ASCOMYCOTA Sordaria fimicola~ ASCOMYCOTA Sordaria fimicola~ ASCOMYCOTA Sordaria fimicola~ ASCOMYCOTA No X-over How many DID NOT crossover? Sordaria fimicola~ ASCOMYCOTA Did X-over How many DID crossover? Sordaria fimicola~ ASCOMYCOTA How many DID crossover? Total counted X 100 Sordaria fimicola~ ASCOMYCOTA 349 609 X 100 Sordaria fimicola~ ASCOMYCOTA . 573 X 100 = 57.3 Sordaria fimicola~ ASCOMYCOTA Divide this number by 2 How many DID crossover? Total counted X 100 Sordaria fimicola~ ASCOMYCOTA . 573 X 100 = 57. 3 / 2 Sordaria fimicola~ ASCOMYCOTA = 28 .65 Sordaria fimicola~ ASCOMYCOTA = 28 .65 this means 28.65 MAP UNITS Sordaria is a haploid organism for the majority of its life cycle It becomes diploid only when mycelia fuse and two different types of haploid nuclei form a diploid nucleus.