A 3-week Biotech Unit
for Any Biology Class
Ellyn Daugherty
SMBCP, San Mateo CA
Educator/Author
www.BiotechEd.com
www.SMBiotech.com
www.emcp.com/biotech
www.sargentwelch.com/biotech
Biotech in Biology
3 weeks (15 hours)
> What is Biotechnology? Computer Search Collage
> Micropipeting Activity
> DNA Spooling Activity
> DNA Fingerprinting Using RFLPs Activity
> Recombinant DNA Technology
> Bacterial Transformation (GMO) Activity
> Bioethics of Engineering Organisms
> Careers in Biotechnology
Materials, Supplies, Funds Needed
for Biotech in Biology Unit
Class set of:
•
4 micropipets/tips each (P-1000, P-200, P-20)
•
4 gel boxes, 2 power supplies
•
DNA Spooling Activity Kit
•
DNA Fingerprinting Activity Kit
•
Bacteria Transformation Activity Kit
•
Other supplies and materials (96-well
plates, food coloring, 37°C incubation
oven, water bath
= about $300 in kits/
class
= about $3000 for
school set of
micropipets and gel
apparatus
What Is Biotechnology?
Micropipeting Activity
Show me:
Pipet
135 µL
or
235 µL
into a tube
DNA Spooling Activity
What Does DNA Look Like?
(Edvotek #S-10)
= one 50 min class period (30 min
activity, 20 min discussion
$39 / 10 lab groups
Do Onions Have DNA (or other fruits
or veggies) ?
(Edvotek #S-75)
= one 50 min class period (30 min
activity, 20 min discussion
$49 / 5 lab groups
… or use my Lab 4b Materials from SW List
DNA Fingerprinting Using RFLPs Activity
Ward’s DNA Detectives Lab Activity
#109)
= four 50 min class periods
$115 / 8 lab groups
Edvotek’s
DNA Fingerprinting by Restriction
Fragmentation Patterns #109)
= four 50 min class periods
$79 / 6 lab groups
Recombinant DNA Technology
use http://www.dnalc.org/resources/animations/
Use the Restriction Digestion
animation to discuss:
•
Restriction enzymes
•
DNA ligase
•
Recombinant DNA (rDNA)
Use the Transformation animation
to discuss:
•
Insertion of rDNA vector
•
Selection and Expression of
newly insert genes
•
Applications of rDNA technology
Bacterial Transformation (GMO) Activity
Ward’s Improved Bacterial
Transformation Using GFP
#36 V 6359
= four 50 min class periods
$85/ 8 lab groups
Edvotek’s Transformation with Green
Fluorescent Protein
#223 and #969
= four 50 min class periods
$110/ 10 lab groups
Biotechnology Career Research e-Project
Bioethics of Engineered Organisms
Materials
Lists
www.sargentwelch.com/biotech
Find your Sales Rep for
materials/equipment/
support
Biotechnology: Science for the New Millennium
Ellyn Daugherty
ellyn@BiotechEd.com
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Text with Encore CD
Lab Manual
Instructor’s Guide
Course Planner (Lesson
Plans)
• Instructor Resource Center
on web
• Student Notebook, Test
Generator, etc. available
www.BiotechEd.com for tour through the entire curriculum,
ancillaries, websites, and professional development opportunities