Cell Signaling Tidbit Overview Course: Introductory Cell Molecular Biology. Unit title: Cell Signaling – The Original Social Networking. Class enrollment: 25 – 250 freshman or sophomore Student experience: Near the end of the term. Within the signal transduction unit Homework and pre-class assessment to encourage participation: Review cell signaling (notes and previous class content) and turn in written responses to LOC/HOC questions. Sensing the Rainbow: Demonstration of signal detection and transduction • • • • Nose pinched Chew skittle Let go of nose What happens? Sensing the Rainbow: Demonstration of signal detection and transduction • • • • Nose pinched Chew skittle Let go of nose What happens? Olfaction detection is based on GPCRs - Activity and Outcomes • Activity: Dramatize a signal transduction pathway • Outcome: The students will be able to model a hypothetical signal transduction pathway to elicit a specific cellular response What we want you to know and do • Evaluate the G protein coupled receptor pathway • Identify key components of cell signaling pathways • Design a skit to illustrate the mechanics of the pathway • Predict how the pathway would change under different conditions Timing of Tidbit Min Activity 0-2: Introduction and demonstration 2-7: In your NASI group evaluate your returned homework (total 8 groups) 7-21: Two groups merge, to plan performance (total 4 groups) 21-26: Combine 2 planning groups; perform/critique (total 2 groups) 5 Min: In your NASI groups look at your returned homework handout. Evaluate this piece of work using the following rubric: 13 Min: •Two groups merge (now 4 groups in total) •Create dramatization to illustrate pathway •Suggestion – divide the group into performers and a director 5 Min: Dramatime! Again pair with another group Within pair, one group performs, the other critiques (using Rubric provided) the performance Wild Card 1 Beta blocker – examples Inderal or Lopressor • Blocks the binding site for epinephrine on the beta-receptor • What is the effect on your signaling pathway? Wild Card 2: Caffeine Inhibits the phosphodiesterase that breaks down cAMP. How does this affect your signaling pathway? Wild Card 3 - Anthrax One toxin in Anthrax (EF) Increases cAMP levels Another (LF) triggers the degradation of the kinase further downstream Student Outcomes • Were you able to evaluate and construct a complete signaling pathway based on other students’ homework? • Were you able to create an accurate skit that showed you learned the pathway? • Did you see how altering the pathway affected signal transduction? • Did acting out this cellular process deepen your understanding of cell biology? Professor Outcomes • Did the students achieve the learning objective? – Modeling a hypothetical signal transduction pathway that elicited a cellular response? • Make notes in Teacher Diary of the successes and problems with executing the activity.