and at AMERICAN MUSEUM OF NATURAL HISTORY, New York, USA April 21, 2014 Brief Description of the Equipment • Knife • Chopping Board • Weighing Scale • Measuring Glass • Kettle • Basin • Hand Sprayer • Stove - used for slicing the leaves into smaller pieces - a board where leaves are sliced into smaller pieces - weighs the amount of leaves needed for decoction - measures the volume of water needed for decoction - container used for decoction of the mixture - used as container for the sliced pandan leaves - used as application tool - supplies heat for decoction General Procedure Extraction 1. Researcher gather pandan (Pandanus odoratissimus L.) leaves to be sliced into smaller pieces using knife and chopping board. General Procedures 2. Weigh the amount of smaller pieces using the weighing scale into the basin and put it in the kettle with an amount of water using the measuring glass. General Procedures 3. Heat the kettle with the use of the stove for thirty minutes (30 minutes) for decoction. General Procedures Treatment 1 Partial picture of the respondent using the first treatment in trial 1 with 1 kilogram of pandan leaves that decocted in 1000 mL of water. Results and Interpretation of the Data Table 1.1 Treatment A of Pandan extract to Dog No. 1 TREATMENT 1 (1 application of treatment/day) Decoction PANDAN EXTRACT USED IN TREATMENT (mL) NUMBER OF REPELLED FLEAS PANDAN LEAVES (Kg) WATER (mL) Trial 1 1 1000 400 37 Trial 2 1 1000 400 25 Trial 3 1 1000 400 23 Trial 4 1 1000 400 19 Trial 5 1 1000 400 16 Results and Interpretation of the Data Table 1.2 Treatment B of Pandan extract to Dog No. 2 TREATMENT 2 (1 application of treatment/day) Decoction PANDAN EXTRACT USED IN TREATMENT (mL) NUMBER OF REPELLED FLEAS PANDAN LEAVES (Kg) WATER (mL) Trial 1 1 1500 400 34 Trial 2 1 1500 400 22 Trial 3 1 1500 400 19 Trial 4 1 1500 400 17 Trial 5 1 1500 400 15 Results and Interpretation of the Data Table 1.3 Treatment C of Pandan extract to Dog No. 3 TREATMENT 3 (1 application of treatment/day) Decoction PANDAN EXTRACT USED IN TREATMENT (mL) NUMBER OF REPELLED FLEAS PANDAN LEAVES (Kg) WATER (mL) Trial 1 1 2000 400 30 Trial 2 1 2000 400 20 Trial 3 1 2000 400 18 Trial 4 1 2000 400 13 Trial 5 1 2000 400 9 Results and Interpretation of the Data • It shows that in every trial, there were decreasing numbers of repelled fleas. The dog was treated once a day in 5 consecutive days as represented by the number of trials. There is an evident decrease on the number of fleas on the dog after every treatment Results and Interpretation of the Data No. Of Fleas Repelled 28% 38% Treatment 1 Treatment 2 Treatment 3 34% Results and Interpretation of the Data Conclusion Recommendations • That the pandan leaf extract be tested to a wide variety of host animals that are also susceptible to dog fleas (Ctenocephalides canis) like domestic cats and other mammals. Recommendations • That in the depth study on the flea-repelling properties of Pandan (Pandanus odoratissimus L.) be undertaken hence the determination of the active repelling substance. The Presentation Adviser Mr. Rolando R. Barcelon, (Master Teacher I of the Science Department) QHS SEED Coordinator for Student Affairs The Presentation Co-adviser Mrs. Lucila V. Dizon, (Teacher III of the English Department) Our very energetic, approachable, and supportive, QHS SEED Coordinator for Communication The Presentation Co-adviser/Coordinator Ms. Rosalina L. Molina, (Master Teacher II of the Mathematics Department) Our very friendly, dynamic, approachable, and energetic, QHS SEED Coordinator The Presentation Co-adviser/Coordinator Ms. Madeline S. Cuevas, (Teacher III of the Science Department) Very benevolent, friendly and energetic science teacher Mrs. Carolyn C. Simon (Head Teacher III) & Ms. Cecilia H. Paguia, (Head Teacher III) our Science and Math Department Heads respectively for their sustaining guidance and support Our loving, kind and approachable school principal Dr. LEVITA U. RAMOS School Principal IV, Quirino High School, QC Phil. Our very supportive Ma’am Corinne Damas-Sandiford Project Manager, Schlumberger SEED Paris Our supportive, kind Ma’am Gloria Bato, SEED Coordinator, Philippines Schlumberger Excellence in Educational Development (SEED) and staffs headed by Simone Amber for the magnificent opportunity given to us to share and the benevolent contributions in educating the youth worldwide; special thanks to Ma’am Monia, Ma’am Susan Randel & Sir Nathan Bellomy for their help and encouragement. friends, classmates, schoolmates; Si-SEED-lan Club for the continued wisdom, strength or blessings showered to to us to finally present this presentation internationally.