Image-Pro Premier
Basic Training Course
Part 5 - Automated Counting
Automated Counting
Manual measurements take time and are difficult to
repeat reliably
Objects in images often share intensity characteristics
that are different from background or other object
By identifying groups of pixels based on pixel
information we can automatically measure objects
Automated Counting
1. Segment image
2. Set up counting option
3. Select measurement types
4. Count
5. Display and save/export data
Segmentation
Divide pixels from the image into segments
corresponding to:
Objects or one or more class
Background
Image-Pro can automatically identify bright or dark
objects
Manual segmentation give more flexibility
Automatically
Segmentation
Go to Count/Size ribbon
In Segment section
select Bright or Dark
Pixels identified
according to the the
selection are highlighted
on the image
Counting Options
Display Options - Affect
look of objects
Segmentation and Count
Options - Affect how
objects are measured
Connectivity
4 Connect
8 Connect
Only pixels sharing a side are Any neighbouring pixel can be
touching
touching
Clean Borders
Clean Borders
% Surface Area - Partial objects not important, holes
probably need to be ignored - Clean borders > None
Morphology - Only whole objects to be measured, holes
likely part of the objects - Clean borders > All
Counting - Need to avoid double counting partial
objects, 1/2 objects on borders - Clean borders > 2
Adjacent
Measurement Types
Same as manual measurements
Select measurements relevant for object type
Use measurements for filtering
Displaying Data
When objects are counted, measurement
table is automatically shown
Use Measurements Histogram to show spread
Use classification to enhance display on
Image
Exporting Data
Quick Save for Publication to save image with overlays
Quick Save for Analysis to save image with
measurements for review later
Export function in Measurements table to save data to
Excel, a text file or for importing into Premier