Name:
Date:
Rubric:
The order is assessed, as well as the proper presentation of the graph (ensuring the heat map or 2D
graph is visible appropriately), the code structure, labeling of axes, using appropriate units for each axis,
and a critical analysis for each question.
Context
Enzymatic hydrolysis followed by enzymatic esterification enables using soybean oil resulting from food
frying as feedstock for the production of high-quality biodiesel. The percentage of hydrolysis is estimated
by monitoring soybean oil concentration (measuring fatty acid methyl esters with HPLC).
Ref: https://doi.org/10.1016/j.cep.2020.108131
Supposed that the reaction catalyzed by Lipases enzymes is reversible and can be described as follows:
𝐾
3B ↔𝐾𝐵−𝐵 2R
At a constant temperature of 30°C, the activation energy for the forward reaction is unknown. The
activation energy for the reverse reaction is 80 kJ/mol. The known Arrhenius constants are
R=8.31 J K-1 mol-1 and A=6.37*1016 s-1
You have experimental data regarding the catalytic constant of the forward reaction and temperature,
which can be found in the Excel file “PL1_data”.
1) Find the activation energy Ea for the forward reaction using Python. (10 points)
2) Create a 2D plot of the rate law (-rB) as a function of reactants concentration (range of
concentration (mM): [0.2, 1]) using python. (15 points)
3) What conclusions can be drawn regarding the influence of [B] and [R] on the reaction rate?
Which exhibits a greater impact? Use visualization tools to explain your ideas and restricting
your explanation to 200 words max. (10 points)
4) What are the equilibrium concentrations of B and R? (15 points)