Label the following parts of the cell – Is this Gram Negative or Gram Positive?
100 µm
TEM
Label the following parts of the cell – Is this Gram Negative or Gram Positive?
100 µm
Type of Microscopy?
You study Pseudomonas fluorescens, and have identified a single DGC that
signals to a single effector protein to produce biofilms. You then begin work in
the related species Pseudomonas aeruginosa to due its clinical significance.
You identify a homologue of both the DGC and effector. You delete both genes
at once, and find that biofilm formation is only reduced by 50%. List two
possible reasons this may be the case, and what kind of experiment would test
your hypothesis.
You believe you have discovered a large protein complex that binds the
nucleoid of a bacterial cell to help with DNA packing. Which of the
following is the BEST microscopy technique to observe this?
A) Cryo-EM
B) Darkfield
C) TEM
D) SEM
E) Fluorescence
If facultative anaerobe such as Escherichia coli, does not have oxygen as an electron acceptor for
the electron transport system, it will die.
a. True
b. False
If a bacterial cell is discovered at the bottom of the
ocean near a volcanic vent, which terms could
be applicable?
a. Barophile
b. Hyperthermophile
c. Halophile
d. Mesophile
e. A and B
Could you apply Koch’s Molecular Postulates to a prion? Why or why not?
A viroid?
You go digging through a retired Professor’s -80 C freezer looking for interesting bacterial strains that
have been saved to her collection. You discover an intriguing bacterium that is noted as not crosslinking its peptidoglycan layers. You wish to study this bacterium, but unfortunately the label with its
origin has rubbed off.
Which of the following locations is the most likely to have been the environment this bacterium came from?
a. A deep sea hydrothermal vent
b. A salt marsh
c. Pipes that deliver tap water
d. A glacier
e. An intracellular pathogen of an animal
You grow two cultures, one is inoculated with a bacterium that had been growing for 6 hours
When it was harvested, the other from a culture that had been growing for 60 hours when it was harvested.
Which new culture will have grown more after another 6 hours have passed? Why?
You want to test the ability of a mutant bacterium to swim, so you make K10T-1 plates
You inoculate the plates and discover the mutant strain doesn’t swim, but neither does WT, which is known to.
Unfortunately, you didn’t check the M&Ms before making the plates, and made them with a standard 1.5% agar.
Could this explain your result? Why or why not?
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