Juine T. Ramos
MT IIL -44
Education
College
ofHealthSciences
Floor,
DPTBuilding
MatinaCampus,
DavaoCity
Telefax:(o82)
Phone No.:(082)305-o640/300-0647 Local117
UMI
TheUniversity
of Mindanao
Laboratory
Activity
1
LABORATORYSAFETYAND PROFESSIONALCONDUCT
Introduction
Human Anatomy and Physiology,
especially
when studied alongside
Pathophysiology,
involves
exposure
tolaboratory
settings
wherebiological
specimens,
body
fluids
(e.g.,
blood,
urine),
chemical
reagents,
and sharp instruments
are commonly used.
Theseconditions
presentpotential
risksforcontamination,
infection,
and injury(Pasco,
2021).
As futuremedical
laboratory
professionals,
students
must learnand demonstrate
responsible,
ethical,
and safelaboratory
conduct
(Ingham,2023).The practice
ofsafety,
aseptic
technique,
biosafety,
and biosecurity
notonlyprotects
thestudent,
butalsotheir
colleagues,
instructors,
patients,
andtheenvironment
(Ernstmeyer
etal.,
2021)
Thislaboratory
activitywillserveas an essential
foundation,
ensuring
students
understand
how to handleanatomical
models,human-derived
specimens,andpotentially
infectious
materials.
The goal istodevelophabitsand protocols
thatprioritize
safetyand
professional
integrity
throughout
thecourse
and their
future
clinical
practice.
KeyTerms:
laboratory
safety,
aseptic
techniques,
biosafety,
biosecurity,
infection
control,
PPE,waste
segregation
Objectives:
Attheendofthis
activity,
students
areexpected
to:
1.Identify
andpractice
safelaboratory
conduct
jn-ahealthcare
laboratory
setting.
2. Apply basic aseptictechniques
in handliag
biological
specimens
a
nd anatomical
materials.
3. Describe
theconcepts
andimportance
of biosafety
and biosecurity
inlaboratory
and
clinical
settings.
Materials/Equipment:
PerStudent:
Laboratory
gown
Head cap
PerGroup:
Handwashing station
or
sanitizer
Disposable
Bleachor 70% alcohol
gloves
Facemask (sirgical
or N95)• Soap/detergent
Cleaning
sponge,
tissue,
orrag
Biohazard
wastebag
Safetygoggles
Anatomical
models(fordemo
College
of Health
Sciences
Education
UM
3"Floor,
DPTBuilding
MatinaCampus,DavaoCity
Telefax:(o82)
Phone No.:(082)305-0640/300-0647
Local17
TheUniversity
of Mindanao
Procedures:
A. Understanding
KeySafetyPractices
inthe Laboratory
1.Donning
and Doffing
ofPPE
o Demonstratethecorrect
sequence
ofputting
on (donning)
andremoving
(doffing)
PPE.
o Emphasize
avoiding
self-contamination
during
removal.
2.HandHygieneandHandwashing
Protocols
o Performthe7-step
WHO handwashing
technique.
o Discuss
when to usealcohol-based
sanitizer
vs.
handwashing.
3.SafeBehavior
and ConductInside
theLab
o Noeating,
drinking,
applyingcosmetics,
orusing
phones.
o Maintain
silence
andavoid
o Observe
instructions
unnecessary
movements.
strictly
during
specimen
handling.
4. ProperUseand Disinfection
ofWork
Surfacesand Tools
Disinfect
work surfacesbeforeandafterlabwork
using
bleach
or70% alcohol.
o Cleananatomical
modelsand
o
non-disposable
instruments
post-use.
5.Aseptic
Techniques
Application
o Use sterile
gloves
andavoidcross-contamination
bylimiting
surface
contact.
o Avoid
touchingyourfaceorpersonal
itemswith
glovedhands.
6.Waste Disposal
Protocols
o Segregate
biohazard
(e.g.,
gloves,
cotton,
sharps)
fromgeneral
o Label
waste.
andsealbiohazard
bagsproperly.
B.Introduction
to Biosafety
and Biosecurity
inA&P Labs
1.
Biosafety
Levels
(BSL)
–Overview
ofthe
4 levels
andtheir
relevance
2. Biosecurity
todiagnostic
inMedicalTechnology
labs.
o Emphasize
securestorage,
use,
a
nd
disposal
ofhuman-derived
o Avoid
specimens.
unauthorized
accessor sharing
ofmedical
3.CaseDiscussion:
specimens.
Historical
Lessons
from UnsafeLabPractices
o Briefly
present
examples
(e.g.,
lab-acquired
infections
inearlymicrobiology).
o Connect
withcurrent
infection
control
standards
(e.g.,
COVID-19labsafetyupdates).
OUMI
cesEducation
Scien
College
ofHealth
"Floo,DPTBuilding
DavaoCity
MatinaCampus,
Telefax:(o82)
TheUniversity
of Mindanao
PhoneNo.:(082)305-064300-0647 Local117
.
Post-Laboratory
Questions:
1. Definedonning
anddoffing.
How canyouobserveproperdonninganddoffing
inside
thelaboratory?
Why isthis
significant?
Ponle
oip olletpalproechve
to upnu(PP),
Donpin Ar Penovhy
henity eperoennngonddorngnaabraty
folaerhbheelonroh
cnuehlnr
ENee E pgperd hedonieoed)Dog E hapec/rcdrdeg. soan,at9ogle
gbve)
Dogmns
E
n peoje Order (eg,glove,
goun,
gugles,
mat) 5)Avod tuchingcontbmied
i
pptcor-
Sgaifcance:
Poper dorming oud dring cuca o
Aatarnoroerehlabratny
- egwe
lnec
honr.
prut
miainieenkr and enare
a
ape
working
conlamincahon,
ng poch
we
ca
tnvionnent
2. Illustrate
and explain
the process
of standardhandwashingprocedure.
What isthe
significance
ofproper
handwashing?
farcand
Hand Nahing Pocedue
2
hoydr
Wet
3) Rub
Mh
har
poln palm
to
Crpetfrgetf
ngh
vm
pln ahd'wice
with wakr
2)Apply enomghvaap to
Caer al hard iraces
backuard anl
fornarcr
2) Rotationa/
hubbing,
fngersiertaced
G)backrof fingerr ogparing
Palmç wihfngers
inertoded
Imelo
cked
h
Aotahional
bing,
c/oape
in nightpolytand
Mhnt
e
vexo
9)Rinse han
wih Wade
o)Dn hadronathlyw geR ae
b) Yonhande are
1
Rightpalmover
lept dorinm
on
herlced fherr
wh
hondn el
|DraHn q
ngw
he entie proedure
ic o-G0 secncs
:
PoperHanduahing
Vgnipcanceo
Pcyentlhfeetomr:
Kenpve Pa/ogenreucina
kr eer hd hnnoiorcn.
2) ouce diseotespad:trechve dgaing
a
wid
range eg
mitroorganim
2)Pomfe ealh
Lrentdfrrperonaland AublicHealth
9) Critical h Mgh Prt sehig talhcan,
frathancng,
and
chilad
care
3.Are thereany sandardized
practices
of aseptictechniques
inPhilippine
healthcare
settings?
Elaboráte.
UM
College
of Health
Sciences
Education
3Floor,
DPTBuilding
Matinaampus,Davaoity
of Mindahae
The University
Telefax(o82)
PhoneNo.:(082);05-064300-0647 Local117
ncey
Atepte Nom touchtechniguc
(AMT),
:
Aohe thnlave HondHygiene,bPeCPntonal
proteivetyupma),
Areptic
Managemn!.
Slere
wipnent,
egnar
(Pee
Yanng
o7
DCATION
gph
RpAAC
ronie
-
AAN- 7DMCH
Thh
èl
n0nkey patr
honpeerce
nor
Parmmar
) Mwayr warhhand efredevely.
) Nevr
)
TRCHNIQME (ANTT):
e
ppopn
ctol. Me
7NDADISED PRDCTILET :-PnviroMnetol
o /0ecthepreafior(ontominclepot
fJe
Glover,
- Drnpech cd
fog
Jenif7cHron
4. Conclusions/Insights:
Refecton what you learned
from thislab.Why is safetyimportantinanatomyand
physiology
laboratory
settings?
How can these practicesbe applied
in real ciinical
environments?
to
taureraccurak eruk,
2) #
responihehandng of oecnear and toupnint.
n
bwirrnhuentr:
Cinlcd/
pplcak
pruventshealhcare-aaocaketlpechion.
woreerT.
proth patentrand Mahca
An/e
9
piomo
/bhnr done d
eecnCare.
References:
&
(Chapter4: Aseptic
E.(Eds.).
(2021).Nursingskills
Ernstmeyer,K., Christman,
Technical
College.
Chippewa
Valley
technique).
https://www.ncbi.nlm.nih.gov/book/NBK593203/
Pasco, E. K.(2021, Septembér6). Are pathophysiology
and pathology
the same?
Getaprofessor.
https:/getaproféssor.com/are-pathophysiology-and-pathology-the-same
Ingham,P.
(2023,December).
Crafting
character
andcultivating
professionalism
inaspiring
TheAmericanSociety
andtechnicians.
ASCLS Today, 37(6).
medical
laboratory
scienísts
forClinical
Laboratory
Science.
https://www.ascls.org
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