lOMoARcPSD|7929783
enzyme cheat sheet
Biochemstry and molecular biology (University College London)
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Downloaded by Samantha Pahl (pahlsamantha@gmail.com)
lOMoARcPSD|7929783
Lineweaver-Burk
ENZYME
SHEET
CHEAT
KINETICS
plot
gradient
y-int
-in+,
↑ Km
,
y
x =8
,
= 0
X Vmax
binds to E-S complex
↓ Km
dVmax
,
effec6 &* [1]
X0Km
↓Umax
,
equanun
OmDunco was e
enzyme efficiency/specificity constant
Michaelis-menter
complex
km
=
(k
-
kz)
+
,
Kat
K,
Km
=
Vo
Km
"Umax
<S] at
turnover # (RoR with
enzyme)
Umax[S]
[S]
km
=
Keat
=
+
[Ed
5 assumptions
1
2
.
.
.
3
in
Steady
ES
>
-
-
negligible
E + P- ES
state
,
-2
so
,
constant
E + P slowest.
rate
Key
[S] almost
.
5
[Er]
Keat
also ,
=
=
-
-
Km
,
measured
is
the
is
a
under
activity
[E] + (ES]
measure
of
the
conditions
units)
purity of
always
km
Ka
lenzyme
in
a
miligram of protein
enzyme
=
mechanism
Ka
Ka
determining
unchanged
standard
M-M
Keat
[ES]
SPECIFIC ACTIVITY
~
classic
ignored
~
4.
Umax
.
preparation
app
L
presence of inhibitor
Downloaded by Samantha Pahl (pahlsamantha@gmail.com)
=
smaller
or
O
Aka
equal
Ka
to
=
km
L
as