Downloaded from http://jramc.bmj.com/ on May 10, 2015 - Published by group.bmj.com ORIGINAL PAPERS Effects of an Eight Week Military Training Program on Aerobic Indices and Psychomotor Function JP Hickey1, B Donne2, D O’Brien3 Medical Officer, Irish Defence Forces, Army Medical Corps; 2Department of Physiology, University of Dublin, Trinity College; 3 Clinical Psychologist, Irish Defence Forces, Army Medical Corps. 1 Abstract This study assessed the effects of eight weeks of military training on aerobic fitness indices, military skills and neuropsychological function. Thirty five (n=35) male Irish Defence Forces personnel, divided into training (n=20) and control (n=15) subgroups, completed tests of military aptitude (Kim’s games, judging distance, fire order, map reading, weapon assembly) and neuropsychological function (Symbol digit modalities test (SDMT), Trail making test, Stroop test and grooved pegboard test) pre- and post-intervention. The repeated measures study design sought to account for any learning effect. Participants also completed a 10km route march, a two mile run and three by 20m shuttle run tests at both time points to quantify changes in fitness variables. The training sub-group significantly (P<0.001) improved mean 20m shuttle-run distance and consequently estimated VO2 max pre- to post-intervention (49.8±1.0 vs. 52.4±0.9 mL.kg-1.min-1). Two mile run time was not significantly improved. Mean %HRmax during the 10km route march was significantly higher in both training (P<0.001) and control (P<0.01) sub-groups post-intervention (71±1 and 83±1%) compared to pre-intervention (65±1 and 77±1%). However, the training sub-group conducted the route march at a significantly faster speed on the second occasion. Military training significantly improved performance in 3/18 neuropsychological test components and 2/12 military skills test components. Training significantly improved ability to estimate both short (error; 36±6 vs. 12±1%) and intermediate (error; 72±12 vs. 11±3%) distances post-intervention. The training sub-group significantly (P<0.01) improved SDMT score and mean Trail 1 time pre- to post-intervention (58.0±2.8 vs. 69.5±3.4; 18.1±0.8 vs. 14.4±0.8s, respectively). In Part 3 of the Stroop test, time mediated a significant (P<0.05) and selective improvement in the training sub-group (51.3±3.2 vs. 63.8±5.4). In conclusion, aerobic fitness and a minority of neuropsychological and military skills tests improved following 8 weeks of military training. Introduction The beneficial effects of exercise on cognition have been the subject of speculation since antiquity. While the notion of “mens sana in corpora sano” is far from universal, there is little doubt that a positive relationship exists between chronic aerobic exercise and improvements in overall cognitive capacity [1]. Chronic exercise associated with an increase in VO2max, defined as maximal oxygen uptake or aerobic capacity of an individual and measured in mL.kg-1.min-1 [2], is thought to increase oxygen saturation in areas of the brain crucial for task performance [3]. The cerebral circulation hypothesis proposes that higher levels of aerobic fitness result in increased cerebral blood flow and oxygenation as a consequence of higher VO2max. The increased availability of oxygen to brain cells allows them to work more efficiently [4] and increased cerebral capillary growth has also been implicated [5]. While a recent meta-analysis questioned the direct causal role of physical fitness in the augmentation of cognitive performance, 86% of the studies in question demonstrated a positive correlation. However, the role of confounding factors was considered to be significant in mediating this effect [1]. Corresponding Author: JP Hickey, St Bricin’s Military Hospital, Infirmary Road, Dublin 7, Ireland Tel: +353 87 6982333 email: hickeyjohnpaul@gmail.com J R Army Med Corps 158(1): 41-46 Whatever the relationship, the interaction of physical activity and cognitive processes is particularly relevant to military personnel, as they are required to maintain a high level of physical fitness and a cognitive functional reserve during physical exertion in order to carry out their duties effectively. Executive control, reaction time and fine motor function are particularly relevant traits. Collectively, they constitute the ability to differentiate and select appropriate responses from a pool of potential but inappropriate responses to a given stimulus, to react quickly to such a stimulus and to affect a motor response. The soldier in training is a suitable research model to examine this relationship. Military training combines physical, academic and technical facets, and physical training in the military often incorporates technical or tactical scenarios. From the recruitment process through to the syllabus of training and the methods of instruction, the goal of military training is to prime the soldier to function effectively on a number of levels. The Irish Defence Forces Three Star training syllabus provides a homogenous cohort of participants who have already undertaken 16 weeks of recruit training and are well versed in fundamental military skills. The effect of further training on these modalities can be gauged by comparing against controls who are not yet engaged in further training. Much research conducted in the military concentrates on performance of tasks in adverse conditions such as extremes 41 Downloaded from http://jramc.bmj.com/ on May 10, 2015 - Published by group.bmj.com Military Training and Psychomotor Function JP Hickey, B Donne, D O’Brien of cold [6-8], heat [9], dehydration [10], sleep and nutritional deprivation [11]. The specific interaction of military training with aerobic fitness and cognitive processes relevant to performance of military tasks in the field is not addressed in these studies. We hypothesised that an eight week military training program would induce an improvement in the level of participants’ physical fitness, a potential marker for an associated improvement in cognitive function. We also hypothesised that such an improvement in cognitive function would not only be reflected in the performance of military specific tests, but would be demonstrated by improvements in ancillary neuropsychological tests. whereas combining training units to increase the sample size would have introduced a number of potential confounders. In addition, normative data for the military skills tests were not available. A power calculation with an α level of 0.05 and a β level of 0.80 inferred that a sample size of 13 per group would be required to detect a change of 1 standard deviation (SD) between groups. Participants within study cohorts were not randomised as both sub-groups were pre-designated. A single platoon (n=27, 1 female) comprised the study sub-group, and all current Air Corps trainees (n=23, 1 female) comprised the control sub-group. No personnel declined to take part and all eligible participants were included. Materials and Methods Physical Fitness Assessment Study permission was obtained from the relevant Defence Forces authorities, standard academic institutions ethical guidelines were adhered to and informed voluntary consent was obtained from participants prior to study commencement. Enrolled participants were assessed by a medical officer in accordance with Defence Forces regulations and completed a short questionnaire to establish basic sociological variables within the cohort. All personnel passed their annual fitness test in compliance with army training instructions. This comprises assessment of body mass index (BMI) and subsequent anthropometry for individuals with a BMI of greater than 30 kg.m-2, press-ups and sit-ups, a 3.2km run and a loaded march over 10km in full battle dress. Those eligible for inclusion in the study were members of the Irish Defence Forces who had completed initial recruit training but not Three Star training, male or female, aged 18-30 yr. Exclusion criteria were injury, illness and those with difficulty in reading, writing or performing basic arithmetic. Since 2006, 566 personnel have completed the Three Star syllabus, approximately 6% of whom were female. Sample size calculations were not performed on the basis that the size of the training group was limited by the nature of military training, which usually occurs in platoons of 30 personnel subject to the same training conditions. Sampling from within the same platoon ensured consistency, Participants performed three 20m shuttle run tests (20 MST) in a gymnasium, a 3.2km run and a terrain walk conducted over 10km carrying a 20kg pack. Outdoor assessments within subgroups were conducted at separate sites, there were no significant gradients, weather was seasonal and conditions varied as expected. Neuropsychological and Military Aptitude Tests A battery of neuropsychological tests was applied (Table 1). These tests were rapidly and easily administered, easily interpreted by a non-psychologist, and accessible across wide social, educational and intelligence ranges. A further battery of tests assessing military skills was also conducted (Table 2). Intervention Three Star training is conducted over a 12 week period (Table 3). There is a strong emphasis on physical training, with 41 hours of physical education, front-loaded to the initial eight weeks of the course. Training outputs include completing a 15km open terrain march, an endurance run over 9km, a 7km march at scout pace and a 10km march in full battle dress. Since the greatest gains in physical fitness were likely to occur during the initial period, assessments were performed during weeks one and two and again during weeks eight and nine. During this period the control sub- Test SDMT [12] Trail making test [12] Stroop test [12] Pegboard test [12] Format Group Group Group Individual Description Single part test. Match series of symbols to digits using a symbol-digit key in 90 s Two part test. Trail 1 Draw lines connecting numbers (1 to 25) in ascending order. Trail 2 Draw lines connecting numbers (1 – 13) and letters (A – L) in an alternating ascending pattern Three part test. Part 1 – Name word (RED) Part 2 – Name colour (XXXX). Part 3 – Name colour with a distraction in place (BLUE) Outcomes Score Errors Trail 1: Time, errors, near misses and prompts Trail 2: Time, Letter errors, number errors, near misses and prompts Part 1 Part 2 Part 3 Interference score Time Drops Total score Variables assessed Divided attention Visual scanning Motor speed Orientation Concentration Visuo-spatial capacity Problem solving ability Cognitive flexibility Attention Fine motor function Manual dexterity Reliability coefficient 0.80 [12] Trail 1: 0.55 Trail 2: 0.75 [13] Part 1: 0.90 Part 2: 0.83 Part 3: 0.91 [14] 0.67 (dominant) and 0.73 (nondominant) [15] Insert 25 identical pegs into randomly orientated grooves as quickly as possible Table 1. Descriptions of the neuropsychological tests used. SDMT (Symbol Digit Modalities Test) 42 J R Army Med Corps 158(1): 41-46 Downloaded from http://jramc.bmj.com/ on May 10, 2015 - Published by group.bmj.com Military Training and Psychomotor Function JP Hickey, B Donne, D O’Brien Test Format Description Kim’s games Group Memorise 20 items in 60 s, immediately recall as many as possible in 60 s , repeat 90 min later Judging distance Individual Short (<100m), intermediate (100250m) and long (>250m) distance, measured by laser range finder. % error = computed Fire order Individual Group – pre-assigned unit. Range – distance to target. Indication – pre-selected marker. Type of fire – heavy artillery Time (s) Score (1–4) Outcomes Delayed recall Memory attenuation Variable assessed Short-term memory Reliability coefficient No data available Short distance error (%) Intermediate distance error (%) Long distance error (%) Visuo-spatial Visuo-spatial perception perception Short-term memory No data available No data available Map reading Individual Participants identify within 30 s point of maximum elevation on an ordnance survey map Time (s) Score (0/1) Weapon skills Individual Candidates identify the groups of the Steyer 5.56mm calibre rifle – trigger, barrel, gunlock, housing and butt – assemble weapon within 60 s Component score (1–5) Assembly score (0/1) Assembly time (s) Visual scanning Fine motor function Manual dexterity No data available No data available Table 2. Description of military tests used Subject Weapons training Physical education Internal security and public order training Advanced unarmed combat Operating in a field environment Battlefield first aid Troop operations with helicopters Ceremonial Time (Hrs) 61 41 31 16 15 12 8 5 Subject Tactical training Map reading CIS Education and development CBRN Field craft Administration Hygiene and sanitation Time (Hrs) 61 33 19 16 12 9 8 2 group were restricted to classroom based and technical activity with recourse to personal and recreational physical training in non-military settings. Participants in the control sub-group received no formal tactical military instruction during this period. Statistical Analysis Data were contemporaneously recorded in hard copy format and transferred to Microsoft Excel. Group data are presented as mean and standard error of the mean unless stated otherwise. Data across group and time were analysed using a two factor ANOVA with time as a repeated measure. Where significant differences were detected across either group or time, post-hoc Bonferroni tests and weighted Student’s T tests were used to identify the subgroup within which changes were detected, for all statistical tests an α level of 0.05 inferred significance. Results Thirty five male participants (mean age ± SD 21±2 yrs), 20 in training (21.1±1.1 yrs) and 15 controls (20.9±2.3 yrs) completed all study elements. Reasons for not completing all elements of testing included non-availability due to unforeseen commitments and injury incurred outside of the study setting. The control sub-group spent an average of 13.9±1.9 yr in formal education compared to 13.5±1.1 yr in the training sub-group. J R Army Med Corps 158(1): 41-46 Mean VO2 max (mL.kg-1.min-1) Table 3. Components of the Irish Defence Force Three Star training programme. CIS ( Communication and Information Services); CBRN (Chemical, Biological, Radiological and Nuclear). Time (pre- and post-intervention) Figure 1: Graph of predicted VO2 max in training and control subgroups pre- and post intervention. Bars denote SEM. Filled bars denote training group; unfilled bars denote control group. χχχ infers significant time within group difference at P<0.001 Aerobic Fitness Indices Mean shuttle run distance and subsequently estimated VO2max, derived using data developed by Ramsbottom [16], increased significantly in the training sub-group pre- to post-intervention 43 Downloaded from http://jramc.bmj.com/ on May 10, 2015 - Published by group.bmj.com (49.8±1.0 vs.52.4±0.9 mL.kg-1.min-1 , P<0.001). No significant differences were detected in the control sub-group (Figure 1). Two mile run time did not change significantly in either sub-group between assessment time points. Both training and control subgroups were working at significantly higher mean percentage of maximal heart rate (%HRmax) during the post- (71±1 and 83±1%) compared to the pre-intervention route march (65±1and 77±1%). However, the training sub-group performed their second route march at a significantly faster speed than their first, while the control sub-group performed a slower pace (Figure 2). JP Hickey, B Donne, D O’Brien Mean SDMT score (arbitrary units) Military Training and Psychomotor Function Mean velocity (m.s-1) Time (pre- and post-intervention) Figure 3 Graph of mean SDMT score in training and control subgroups pre- and post intervention. Bars denote SEM. Filled bars denote training group; unfilled bars denote control group. χ χ infers significant time within group difference at P<0.01. β infers significant group within time difference at P<0.05 Figure 2: Graph of mean velocity measured during 10km route march in training and control sub-groups performed pre- and postintervention. Bars denote SEM. Filled bars denote training group; unfilled bars denote control group. χχ infers significant time within group difference at P<0.01 Neuropsychological Tests The training sub-group recorded a significant (P<0.01) improvement in Symbol Digit Modalities Test (SDMT) score pre- to post-intervention (58.0±2.8 vs. 69.5±3.4), a finding not observed in the control sub-group (Figure 3). Significantly (P<0.01) fewer errors were committed performing the SDMT after intervention. However, the effect was not isolated to either training (from 0.50±0.17 to 0.05±0.05) or control (from 0.80±0.3 to 0.0±0.0) sub-groups and could therefore not be attributed to the intervention of training. A significant time effect (P<0.01) was observed for Trail 1 time (Figure 4). Post-hoc analysis revealed a selective within group difference (P<0.01) for the training sub-group. Their mean performance improved from pre- to post-intervention (18.1±0.8 vs. 14.4±0.8s) compared to a non-significant change in the control sub-group. No other significant effects were observed for either part of the trail making test. The significant time effect (P<0.05) observed for Part 1 of the Stroop test was not exclusive to the training sub-group and no significant effects were observed for part 2. In part 3 of the Stroop test, time appeared to mediate a significant (P<0.05) and selective performance improvement in the training sub-group from pre- to post-intervention (51.3±3.2 vs. 63.8±5.4) when compared with the control group (65.3±5.3 vs. 65.0±3.1). Error commission performing the grooved pegboard test was unaffected by the intervention. A significant time effect (P<0.001) 44 Mean trail 1 time (s) Time (pre- and post-intervention) Time (pre- and post-intervention) Figure 4: Graph of mean trail 1 time in training and control subgroups performed pre- and post-intervention, bars denote SEM. Filled bars denote training group; unfilled bars denote control group. χχ infers significant time within group difference at P<0.01 was observed for pegboard test time. However, post-hoc analysis revealed that this improvement was only significant (P<0.01) in the control sub-group pre- to post-intervention (64.3±2.4 vs. 56.6±1.7s), corresponding times for the training sub-group were 66.2±2.3 and 63.7±1.9s, respectively. This selective and significant (P<0.01) improvement was also manifested in total pegboard score. Military Skills Assessment Both memory attenuation and delayed memory recall components of Kim’s games were unaffected by the intervention. The training sub-group demonstrated significant improvements post-intervention in their ability to estimate short (P<0.01) and intermediate (P<0.001) distances, but not longer distances (Table 4). J R Army Med Corps 158(1): 41-46 Downloaded from http://jramc.bmj.com/ on May 10, 2015 - Published by group.bmj.com Military Training and Psychomotor Function Pre-intervention Variable Control Short distance 32 % error (%) Intermediate distance % 40 error (%) Long distance % error (%) 32 JP Hickey, B Donne, D O’Brien Post-intervention Training Control Training 36 36 0χχ 71 40 8χχχ 21 30 13 Table 4. Changes in distance estimation with training. xx Significant time within group difference for training sub-group (p<0.001), xxx Significant time within group difference for training sub-group (p<0.001) GRIT score was unaffected by the intervention. While time did have an overall significant effect on speed of fire order delivery, this effect was not specific to the training sub-group. A significant overall time effect (P<0.05) was noted for GRIT time. However, post-hoc analysis failed to demonstrate a greater pre- to postintervention sub-group effect in training compared to control. Across time the training sub-group demonstrated a slight deterioration in map reading performance whilst the control subgroup demonstrated a slight improvement, neither change was considered significant. Significant time effects were noted for weapon component identification score (P<0.01), weapon assembly result (P<0.01) and weapon assembly time (P<0.001), these effects were observed across both sub-groups and could not be attributed to the intervention. For weapon component identification score, training sub-group pre-intervention data left little room for improvement post-intervention (4.95±0.05 vs. 4.85±0.11), with a maximum possible test score of 5. In contrast, the control subgroup started from a lower pre-intervention score (3.60±0.41) and recorded a significant (P<0.05) improvement (4.67±0.21) over time. Similar findings were recorded for weapon assembly, pre-intervention score of the training sub-group (0.95±0.05) was unchanged and the control sub-group recorded a non-significant improvement post-intervention (0.60±0.13 vs. 1.00±0.00). For weapon assembly time, although a significant (P<0.05) time within group difference was observed pre- to post-intervention for the training sub-group (37.6±2.6 vs. 26.5±2.2s) a significant improvement (P<0.01) was also recorded in the control subgroup (52.6±5.0 vs.32.3±2.0s) and therefore the improvement could not be attributed to the intervention. Discussion Summary of Results We hypothesised that Three Star military training would induce an improvement in participants’ physical fitness. Our results support the hypothesis with clear and significant improvements observed in 20 MST time, estimated VO2max and faster pace during the post-intervention route march. We also hypothesised that an eight week program of military training would induce an improvement in military skills tests. Twelve variables were assessed but only two variables, judgement of short and intermediate distance, were positively influenced as a result of the intervention. As an overall battery, these tests do not support the hypothesis. It was further hypothesised that military training would induce J R Army Med Corps 158(1): 41-46 improvement in performance of neuropsychological tests. However, a significant effect was observed in only three of the eighteen neuropsychological test components – SDMT score, Trail 1 time and Part 3 of the Stroop test. The significant improvement in Part 3 of the Stroop test must be viewed in the context of the low pre-intervention score in the training sub-group. These results do not support the study hypothesis that the Three Star training intervention improved the military skills or neuropsychological skills of Irish Defence Force soldiers. Interpretation Given the lack of support for the hypothesis provided by our data, one or more conclusions may be reached. It may be that the testing protocol may not be capable of demonstrating relevant changes in performance; relevant changes in performance may not be taking place over the course of the intervention or may have already taken place in an earlier phase of training. Alternatively, the changes in performance deemed relevant by the lead investigator may not be deemed relevant by the training authority. Given that a fundamental course objective is ‘to develop the basic military skills required at unit level’, the latter is not the case. In addition, all personnel in the training sub-group successfully completed the Three Star course; thus completing their training objectives. For some of the military skills, a ceiling effect induced by previous training may account for the lack of improvement. The high pre-intervention scores of the training sub-group left little room for improvement during post-intervention testing in weapon skills, map reading and fire order delivery. The control group, individuals not engaged in military training, had limited exposure to these activities, and consequently starting from a lower pre-intervention score achieved a significant improvement over time. It is also likely that the testing protocol employed was not sufficiently sensitive or specific to detect changes over time. A number of neuropsychological and military skills test components were scored within very narrow ranges, making subtle differences within sub-group and time difficult to detect. In addition, there is no specific evidence to validate the neuropsychological tests used in this population as measures of military aptitude, even though the variables assessed are relevant to the duties of a soldier. Potential Methodological Flaws Potential confounders exist within the study data. Although female personnel comprise approximately 5% of the Defence Forces, only two female volunteers were recruited and neither completed the testing or were included in the data analysis. Although the control sub-group were Air Corps personnel engaged in technical training and the study sub-group were mainly drawn from the army, no significant demographic or sociological differences were identified. During the intervention, it was not possible to account for all activities in the control subgroup, particularly during non-directed time. Some individuals were involved in ongoing sporting activities, training with teams, and while the training sub-group were not prohibited from similar activities they would have had limited time to engage to the level equivalent to the control sub-group. In addition, it was not possible to fully account for the activities of those in the training sub-group. Some modules of the Three Star course directly taught skills which were assessed (map reading, weapon handling) while other skills were indirectly taught and alluded to throughout the course (judgement of distance and fire order). This significantly limits the value of these assessments as markers of aptitude. The 45 Downloaded from http://jramc.bmj.com/ on May 10, 2015 - Published by group.bmj.com Military Training and Psychomotor Function personal motivation of individuals in both sub-groups to practice test elements between the two testing points cannot be accounted for, though individuals were specifically asked not to practice any element of the assessment. An additional concern in using the same neuropsychological battery on more than one occasion was that individuals would learn how to complete the tests more effectively on each subsequent occasion. Performance of the Stroop and trail making tests on multiple occasions has been shown to have a significant and cumulative learning effect [17]. The repeated measures design of the current investigation sought to reduce this effect. Other factors such as diet, hydration and mood were outside the realm of influence of the study. The effect of circadian variation was not accounted for correctly as it was not possible to precisely schedule testing for the same time each day. Practical Applications This study attempted to identify a set of scientifically validated neuropsychological variables which are enhanced by military training and correlate with military aptitude. Such tests could be used to enhance the specificity of the Defence Forces recruitment process as demonstrated in other organisations [7]. The process for retention of valuable personnel may also be similarly enhanced. Indeed several studies [1,18,19] suggest that experience is a significant moderating factor in whether or not exercise impacted positively on cognitive performance. Future Research If the changes in performance which we had hoped to measure had already taken place in recruit training, a similar study in that setting may demonstrate performance changes. There are also deficiencies in the current testing protocol which question its validity. The sample size should be increased in order to detect low level change in performance indices. Prior to further study, additional neuropsychological tests should be trialled. While the current study focussed on functional and behavioural variables, the addition of electrophysiological and biochemical markers of evolving cognitive function merits consideration. In addition, inclusion of participant and instructor reported data would add to the weight of evidence. Conclusions An eight week program of military training induced an improvement in physical fitness as measured by estimated VO2 max and mean speed measured during a 10km route march. The performance of a minority of neuropsychological (3/18) and military skills (2/12) tests improved following eight weeks of military training. The proposed hypothesis is therefore not supported by study findings. References 1. 46 Etnier J, Nowell P, Landers D, Sibley B. A meta-regression to examine the relationship between aerobic fitness and cognitive performance. Brain Res Rev 2006; 52: 119-30 JP Hickey, B Donne, D O’Brien Wilmore J, Costill D, Kenny W. Physiology of sport and exercise, 4th ed, Human Kinetics, Leeds. 2007; p106-7 3. Kramer A, Hahn S, Gopher D. Task coordination and aging: Explorations of executive control processes in the task switching paradigm. Acta Psychol 1999; 101: 339-78 4. McFarland G, Ross A. 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J R Army Med Corps 158(1): 41-46 Downloaded from http://jramc.bmj.com/ on May 10, 2015 - Published by group.bmj.com Effects of an Eight Week Military Training Program on Aerobic Indices and Psychomotor Function JP Hickey, B Donne and D O'Brien J R Army Med Corps 2012 158: 41-46 doi: 10.1136/jramc-158-01-11 Updated information and services can be found at: http://jramc.bmj.com/content/158/1/41 These include: Email alerting service Receive free email alerts when new articles cite this article. Sign up in the box at the top right corner of the online article. Notes To request permissions go to: http://group.bmj.com/group/rights-licensing/permissions To order reprints go to: http://journals.bmj.com/cgi/reprintform To subscribe to BMJ go to: http://group.bmj.com/subscribe/
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