lOMoARcPSD|42578826 57 BIOCHEMISTRY Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 58 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination 2015 Time: 3:00 Hours Subject- Biochemistry Paper- I Maximum Marks :50 Instructions: a) b) c) d) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. Do not write anything on the blank portion of question paper, if written anything such type of act will be considered as an attempt to resort to unfair means Q1. Long Answer questions 2 x 5 = 10 a. Enumerate sources of vitamin D, describe its biosynthesis and explain why vitamin D is called hormone b. Explain the pathway of glycogen synthesis and glycogenolysis. In short explain role of branching and de-branching enzymes Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 3 = 30 Endoplasmic reticulum Fluid mosaic model of biological membrane Electrophoresis Selenocysteine Na+-K+ pump Cytochrome p450 Inhibitors of electron transport chain Biosynthesis of thyroxine Isoenzymes Tyrosinemia Q3. Very Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 1 = 10 Enumerate mechanisms of enzymes action Enumerate functions of glutathione Enumerate antioxidant vitamins Enumerate lipoproteins in human plasma Enumerate mechanisms of enzyme inhibition Enumerate functions of zinc Differentiate between starch and glycogen Why urea cycle is called a bicycle Alkaptonuria Draw structure of cholesterol Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 59 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination 2015 Subject- Biochemistry Paper- II Time: 3:00 Hours Maximum Marks :50 Instructions: a) b) c) d) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. Do not write anything on the blank portion of question paper, if written anything such type of act will be considered as an attempt to resort to unfair means Q1. Long Answer questions 2 x 5 = 10 a. Discuss the elongation steps of translation (protein synthesis) b. Enumerate the various tests of kidney function. Explain how creatinine clearance is superior to urea clearance test Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 3 = 30 Genetic code RNA splicing Gout Dehydration SDA Metabolic acidosis Calculate caloric requirement of a painter Glucose tolerance test Catabolism of pyrimidines Formation and fate of bilirubin Q3. Very Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 1 = 10 Purine ring sources of its components Enumerate normal anion gap acidosis Enumerate steps of DNA replication Enumerate clinical features of kwashiorkor Enumerate abnormal variants of hemoglobin Enumerate types of DNA repair Structure of immunoglobin Enumerate various extracellular buffers Biological role of 2,3 BPG Enumerate different components of colorimeter Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 60 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination Aug-Sep 2016 Time: 3:00 Hours Subject- Biochemistry Paper- I Maximum Marks :50 Instructions: a) b) c) d) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. Do not write anything on the blank portion of question paper, if written anything such type of act will be considered as an attempt to resort to unfair means Q1. Long Answer questions 2 x 5 = 10 a. Enumerate major sources of vitamin K, describe its functions and effects of its deficiency (1+3+1) b. Describe the components of chemiosmotic theory of electron transport chain Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 3 = 30 Cyclic AMP Biological role of calcium High density lipoprotein cycle Biomedical role of vitamin C Role of niacin as Coenzyme Biological importance of peptides Cytochrome p450 Isoenzymes and their diagnostic importance Golgi apparatus Synthesis and biological role of bile salts Q3. Very Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 1 = 10 Formiminoglutamic acid (FIGLU) Name the insulin dependent glucose transporters and their tissue distribution Define beta oxidation Write the normal serum calcium and phosphate levels What is suicide inhibition. Give example List 2 differences between hexokinase and glucokinase List 2 differences between marasmus and kwashiorkor Name the derivatives of tyrosine Hemoglobin S Give two examples of substrate level phosphorylation Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 61 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination Aug-Sep 2016 Time: 3:00 Hours Subject- Biochemistry Paper- II Maximum Marks :50 Instructions: a) b) c) d) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. Do not write anything on the blank portion of question paper, if written anything such type of act will be considered as an attempt to resort to unfair means Q1. Long Answer questions 2 x 5 = 10 a. Discuss transcription, genetic code and post transcriptional modifications b. Explain importance of proteins in human nutrition. Why is SDA of proteins higher than that of major nutrients Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 3 = 30 Role of free radicals in aging Tumour markers IgG Enzyme classification Point mutation Heme catabolism Fluid mosaic model of plasma membrane Markers of acute renal failure Telomere and telomerase Calorie requirements in a person having diabetes mellitus Q3. Very Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 1 = 10 Van den Bergh reaction Value of TmG and renal threshold for glucose Name the markers of bone formation Oxidative stress Define vector Principle of photometry Bicarbonate buffer Wobble hypothesis Orotic aciduria Name the important compounds formed from glycine Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 62 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination 2017 Summer Subject- Biochemistry Paper- I Time: 3:00 Hours Maximum Marks :50 Instructions: a) b) c) d) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. Do not write anything on the blank portion of question paper, if written anything such type of act will be considered as an attempt to resort to unfair means Q1. Long Answer questions 2 x 5 = 10 a. Write a detailed essay on glycogen synthesis and degradation. Give an account of their regulation b. Write an essay on the biosynthesis of urea. How is it regulated? Add a note on the clinical significance of uremia Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 3 = 30 Active transport Describe the biomedical functions and deficiency manifestations of vitamin D Classify lipoproteins and write the functions of each lipoprotein Glycosaminoglycans Describe the stages of absorption, transport and storage of iron in the body Competitive inhibition Role of clearance tests in kidney function Amino acids classification based on their nutritional importance What are isoenzymes? Discuss the clinical significance of different isoenzyme variant of CK Detoxification by conjugation Q3. Very Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 1 = 10 Scurvy Fluorosis Bile salts Factors affecting calcium absorption Isoelectric pH Mitochondria PUFA Phagocytosis Cytochrome p450 Pellagra Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 63 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination 2017 Summer Subject- Biochemistry Paper- II Time: 3:00 Hours Maximum Marks :50 Instructions: a) b) c) d) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. Do not write anything on the blank portion of question paper, if written anything such type of act will be considered as an attempt to resort to unfair means Q1. Long Answer questions 2 x 5 = 10 a. Describe the protein synthesis in prokaryotes. How does it differ from protein synthesis in eukaryotes b. What is balanced diet? Formulate a balanced diet for a moderate working vegetarian and non-vegetarian adult males of 70 kg body weight Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 3 = 30 Mutation Endoplasmic reticulum SDA tRNA Enzyme inhibition Free radicals Cardiac markers BMR Reverse transcription HbA1c Q3. Very Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 1 = 10 Salkowski’s reaction Renal threshold for serum calcium Principle of electrophoresis Acidosis Co-factors Respiratory quotient Hemoglobin buffer Km and its significance Oxidative stress ATPase Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 64 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination 2018 Summer Subject- Biochemistry Paper- I Time: 3:00 Hours Maximum Marks :50 Instructions: a) b) c) d) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. Do not write anything on the blank portion of question paper, if written anything such type of act will be considered as an attempt to resort to unfair means Q1. Long Answer questions 2 x 5 = 10 a. Draw urea cycle. Describe the inherited disorders of urea cycle. Write in brief the biochemical basis of treatment of hyperammonemia b. Describe the β oxidation of fatty acids with its energetics. Write a note on biochemical significance of β oxidation of fatty acids Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 3 = 30 Fluid mosaic model of membrane structure Synthesis and deficiency disorders of vitamin D Derivatives of monosaccharides and their biomedical importance Rapaport luebering cycle Hormones of adrenal gland and their functions Glutathione The regulation of plasma calcium Detoxification od aspirin Irreversible inhibition of enzymes Role of dopamine in Parkinson’s disease Q3. Very Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 1 = 10 Essential amino acids Name of different isomers of glucose Iodine number FIGLU test Menke’s syndrome Reichert-Meissl value Biomedical importance of sorbitol pathway Pellagra Fluoride toxicity Hypernatremia Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 65 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination 2018 Summer Subject- Biochemistry Paper- II Time: 3:00 Hours Maximum Marks :50 Instructions: a) b) c) d) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. Do not write anything on the blank portion of question paper, if written anything such type of act will be considered as an attempt to resort to unfair means Q1. Long Answer questions 2 x 5 = 10 a. Give a detailed account of transcription process. How is it regulated? Give the name of inhibiters of transcription b. Describe the de-novo synthesis of purine nucleotides. Indicate the clinical uses of inhibitors of purine nucleotide synthesis Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 3 = 30 Water intoxication Health hazards Renal regulation by acid base balance PCR (Polymerase chain reaction) Free radicals and anti-oxidants Gout SDA Tumour markers and oncogens Abnormal hemoglobin Kidney function tests Q3. Very Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 1 = 10 Biologically important nucleotides Role of fiber in the body Give clinical symptoms of porphyria Principles of ELISA Biological carcinogens Cardiac markers Reactive oxygen species (ROS) Give four characteristics of genetic code Significance off quality control in lab Central dogma of molecular biology Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 66 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination Aug-2019 Time: 3:00 Hours Subject- Biochemistry Paper- I Maximum Marks :50 Instructions: a) b) c) d) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. Do not write anything on the blank portion of question paper, if written anything such type of act will be considered as an attempt to resort to unfair means Q1. Long Answer questions 2 x 5 = 10 a. Absorption, transport and fate of iron in body b. Write sources of vitamin D and explain the role of vitamin D in health and disease Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 3 = 30 Amphibolic pathways of glucose metabolism Fatty acid synthase complex Mechanism for regulation of blood glucose Detoxification of aspirin GTT Iso-enzymes Adulteration of lipids Homocystinuria ATP synthase Ketosis Q3. Very Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 1 = 10 Vitamin A deficiency disorders Enumerate essential amino acids Normal reference range of cholesterol Specialized product of amino acid metabolism Glycogen storage disorders Name four phospholipids Classify proteins HDL Cell membrane Epimers Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 67 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination Aug-2019 Time: 3:00 Hours Subject- Biochemistry Paper- II Maximum Marks :50 Instructions: a) b) c) d) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. Do not write anything on the blank portion of question paper, if written anything such type of act will be considered as an attempt to resort to unfair means Q1. Long Answer questions 2 x 5 = 10 a. Write a note on purine metabolism. Add a note on gout b. Describe the process of protein biosynthesis. Add a note on post translational modifications Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 3 = 30 Tumour markers Liver function tests Air pollution. Enumerate its effects on health Recombinant DNA technology KFT Porphyrias Immunoglobulin Chromatography DNA repair mechanism Lac operon model Q3. Very Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 1 = 10 Normal reference range of sodium and potassium siRNA histone proteins Oncogens DNA fingerprinting Synthetic nucleotides Lesch Nyhan syndrome Ubiquitin HbS SDA Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 68 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination Feb-2021 Subject- Biochemistry Paper- I Time: 3:00 Hours Maximum Marks :100 Instructions: a) b) c) d) e) f) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. MCQ has to be answered in theory answer book Please write MCQ answer neatly and in serial order with black or blue pen in brackets; for example: - 1. (a) 2. (c) MCQ has to be answered only once, any kind of repetition or cutting or erasing or whitener will be considered as malpractice, such answers will not be counted in marks and action will be taken according to UFM rules of university. Q1. Total MCQs: 20 20 x 1 = 20 1. Intracellular sorting, packing and secretion of proteins is done by (a) ribosomes (b) peroxisomes 2. (b) cysteine (b) dipalmitoyl lecithin (b) bile acid (b) melatonin (b) >= 5.6 (c) phosphatidyl inositol (d) sphingomyelin (c) bile pigment (d) steroid hormones (c) dopamine (d) thyroid hormones (c) >= 6.5% (d) >= 7% (c) 3-ketoacyl synthase (d) 3-ketoacyl reductase Rate limiting enzyme of fatty acid biosynthesis is (a) acetyl CoA carboxylase 9. (d) dermatan sulphate Level of HbA1c diagnostic of diabetes mellitus is (a) >= 5% 8. (c) keratan sulphate All of the following are formed from tyrosine EXCEPT (a) melanin 7. (b) heparan sulphate All of the following are formed from cholesterol EXCEPT (a) vitamin D 6. (d) arginine Which phospholipid acts as a pulmonary surfactant (a) cephalin 5. (c) asparagine Corneal transparency is a function of which mucopolysaccharide (a) chondroitin sulphate 4. (d) golgi complex Which of the following is a semi-essential amino acid (a) serine 3. (c) endoplasmic reticulum (b) acetyl trans acylase Pyruvate dehydrogenase multienzyme complex requires all of the following coenzymes EXCEPT (a) thiamine pyrophosphate (b) C0-enzyme A (c) FMN (d) NAD+ 10. Which of the following cannot serve as a generalized energy reserve of the body (a) adipose tissue TAG (b) liver glycogen (c) muscle glycogen 11. Enzyme which is estimated as an indicator of hypoxia in covid 19 patients is (a) CK-MB (b) SGOT (c) SGPT (d) muscle protein (d) LDH 12. Dicumarol acts as anticoagulant by inhibiting vitamin K epoxide reductase (a) competitively (b) non-competitively (c) irreversible (d) allosterically (c) parathyroid (d) thyroid 13. Which of the following decreases serum calcium (a) calciferol (b) calcitonin Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 69 14. Anti tuberculosis drug INH leads to deficiency of vitamin (a) B1 (b) B2 (c) B6 (d) B12 (c) thioesterase (d) thiolase 15. Ketolysis cannot take place in the liver due to deficiency of enzyme (a) thiokinase (b) thiophorase 16. BAL (british antilewisite) or dimercaprol inhibits the electron transport chain at complex (a) I (b) II (c) III (d) IV 17. Food preservative benzoic acid is conjugated with which conjugating agent to be excreted as hippuric acid (a) glycine (b) glutamate (c) glutamine (d) glucuronic acid (c) essential amino acids (d) glucose (c) propionic acid (d) all amino acids except leucine and lysine (c) melatonin (d) all of the above 18. All of the following are formed with the help of TCA cycle EXCEPT (a) haeme (b) fatty acids 19. All of the following are substrates for gluconeogenesis EXCEPT (a) fatty acids (b) lactic acids 20. Which of the following is formed by transmethylation (a) creatine phosphate (b) epinephrine Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 5 = 50 Biologically important peptides Ketosis Secondary structure of proteins Phenylketonuria Electron transport chain Functions and deficiency of vitamin D Competitive and non-competitive enzyme inhibition Structure of cell membrane Iron deficiency and iron toxicity Phase 2 of detoxification Q3. Long Answer Questions a. b. c. 3 x 10 = 30 Describe the breakdown of protein to urea and explain its significance in a person with impaired liver function A middle-aged obese smoker with family history of heart disease came for a routine check-up to the hospital i. What investigations will be done in lipid profile to assess the risk of heart disease (2) ii. Describe formation of cholesterol up to mevalonate, explaining the significance of the conversion o HMG CoA to mevalonate and how it Is inhibited (3) iii. Name clinical conditions associated with hypercholesterolemia and describe atherosclerosis (2.5) iv. Which markers can be used to diagnose myocardial infarction (MI)? Which enzyme is used therapeutically to dissolve the clot (2.5) A diabetic child came to the hospital with complaints of polyuria, polyphagia, polydipsia. i. What investigations can be done in his blood and urine to assess his glycemic index (5) ii. Describe the regulation of blood glucose (5) Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 70 Madhya Pradesh Medical Science University, Jabalpur Time: 3:00 Hours MBBS First Professional Examination Feb-2021 Subject- Biochemistry Instructions: a) b) c) d) e) f) All questions are compulsory Paper- II Maximum Marks :100 Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. MCQ has to be answered in theory answer book Please write MCQ answer neatly and in serial order with black or blue pen in brackets; for example: - 1. (a) 2. (c) MCQ has to be answered only once, any kind of repetition or cutting or erasing or whitener will be considered as malpractice, such answers will not be counted in marks and action will be taken according to UFM rules of university. Q1. Total MCQs: 20 20 x 1 = 20 1. Termination of the synthesis of RNA molecule is signaled by a sequence in the template strand of the DNA molecule, a signal that is recognized by a termination protein (a) Rho factor (b) sigma factor (c) delta factor (d) epsilon factor 2. The correct statement concerning RNA and DNA polymerases is (a) RNA polymerases use nucleoside diphosphatase 3. (b) excise introns from hnRNA (c) remove Okazaki fragments (d) acts as defensive enzymes to protect the host bacterial DNA from foreign organisms (c) uric acid begins to dissociate at pH above 5.8 (d) it is present in plasma mainly as monosodium urate (b) formation of uric acid stones in kidneys can be decreased by alkalinization (c) 600 ml (d) 1000 ml Minimum excretory urinary volume for waste product elimination in 24hrs is (b) 200-400 ml (c) 500-600 ml (d) 800 ml All of the following features are found in blood chemistry in uncompensated lactic acidosis EXCEPT (a) pH is decreased 9. (d) synthesizes RNA primers (b) 300 ml (a) 200-300 ml 8. (c) unwinds the double helix The daily total body water derived from oxidation of foodstuffs is about (a) 100 ml 7. (b) connects the end of two DNA chains All of the following statements about uric acid is true EXCEPT (a) it can be formed from allantoin 6. (d) all RNA and DNA polymerases can add nucleotides only at 3’ end of the growing polynucleotide chain Restriction endonucleases (a) cut RNA chains at specific locations 5. (c) DNA polymerases can add nucleotides at both ends of the chain The enzyme DNA ligase (a) introduces super helical twists 4. (b) RNA polymerase requires primers and adds bases at 5’ end of the growing polynucleotide (b) bicarbonate is decreased (c) pCO2 is normal (d) anion gap is normal During compensation of respiratory alkalosis, all of the following changes occur EXCEPT (a) decreased secretion of hydrogen ions by renal tubules (b) increased excretion of sodium in urine (c) increased excretion of bicarbonate in urine (d) increased excretion of ammonia in urine 10. Normal quantity of urobilinogen excreted in the feces per day is about (a) 10-25 mg (b) 50-250 mg 11. The normal range of indirect bilirubin in serum is (a) 0-0.1 mg/100ml (b) 0.1-0.2 mg/100ml (c) 300-500 mg (d) 700-800 mg (c) 0.2-0.7 mg/100ml (d) 0.8-1.0 mg/100ml (c) 70-85 ml/min (d) 95-105 ml/min 12. The normal values for creatinine clearance varies from (a) 20-40 ml/min (b) 40-60 ml/min 13. Methemoglobin can be reduced to hemoglobin by (a) removal of hydrogen Downloaded by Aayush Music's (aayushgtbps850@gmail.com) (b) vitamin C (c) glutathione (d) creatinine lOMoARcPSD|42578826 71 14. Alpha fetoprotein level in serum is increased in (a) prostatic cancer (b) hepatoma (c) cancer in lungs (d) nephritis 15. Which of the following enzymes produce a free radical in macrophages (a) SOD (b) catalase (c) GSH peroxidase (d) NOS 16. The minimum daily requirement of proteins in food for a normal healthy adult is (a) 0.2 gm/Kg body weight (b) 0.5 gm/Kg body weight (c) 0.7 gm/Kg body weight (d) 1.5 gm/Kg body weight (c) basal metabolic rate (d) physical activity 17. Which has no role in calculating calorie requirements (a) respiratory quotient (b) specific dynamic action 18. Ratio of carbohydrates, fats and proteins in balanced diet should be (a) 50:40:10 (b) 60:20:20 (c) 50:10:40 (d) 70:20:10 19. A 2-year-old boy presents to OPD with poor eating for past few weeks. Recently he has developed a wound on his leg which shows poor healing. On examination he appears plump but apathetic. He has dry, sparse golden hair and skin appears thick and pigmented. Abdomen is distended. His serum albumin is 1.5 g/dL. What is the diagnosis (a) anorexia nervosa (b) starvation (c) marasmus (d) kwashiorkor 20. The laboratory reports the following ABG: pH 7.33, pCO2 40 mmHg ( N 35-45 ) and HCO3 20 mEq/L ( N 22-26 ), you interpret these results as (a) respiratory acidosis (b) metabolic acidosis (c) respiratory alkalosis (d) metabolic alkalosis Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 5 = 50 Dehydration Nitrogen balance Tumour markers Applications of polymerase chain reaction Gout Eukaryotic post transcriptional RNA processing Types of RNA Water intoxication Give an example of some oncogenic viruses. Name some anti-carcinogens Explain the concept of balanced diet Q3. Long Answer Questions 3 x 10 = 30 a. A 67-year-old female presented at clinic with intense jaundice. She had noted that her stools had been very pale. Laboratory test revealed a very high level of direct bilirubin. The level of alkaline phosphatase was markedly elevated (1+2+2+2+3) i. What is the probable diagnosis ii. Why color of stools Is very pale iii. What will be the status of bilirubin and urobilinogen in urine iv. Why is alkaline phosphatase markedly elevated v. What do you understand by direct and indirect bilirubin b. A person presents himself with untreated diabetes mellitus, he is then treated for acidosis (1+2+2+2+3) i. What is the type of acidosis ii. What is the normal bicarbonate/carbonic acid ratio iii. What will happen to the ratio in this patient iv. How will compensation occur v. What is the role of kidney in regulation of acid base balance in the body c. Classify renal function tests. Describe serum and urine markers of renal functions (4+3+3) Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 72 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination April-2022 Subject- Biochemistry Paper- I Time: 3:00 Hours Maximum Marks :100 Instructions: a) b) c) d) e) f) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. MCQ has to be answered in theory answer book Please write MCQ answer neatly and in serial order with black or blue pen in brackets; for example: - 1. (a) 2. (c) MCQ has to be answered only once, any kind of repetition or cutting or erasing or whitener will be considered as malpractice, such answers will not be counted in marks and action will be taken according to UFM rules of university. Q1. Total MCQs: 20 20 x 1 = 20 1. Which of the following is not essential fatty acid (a) oleic acid (b) linoleic acid 2. (b) glycine 3. The main source of reducing equivalents NADPH for lipogenesis is (a) pentose phosphate pathway (d) glycogenolysis (b) estrogen (c) FSH (d) glucocorticoids (b) 2,4 dinitrophenol (c) NADP (d) urea (b) acid phosphatase (c) amylase (d) alkaline phosphatase (b) ribosome (c) peroxisome (d) golgi apparatus (c) dipalmitoyl lecithin (d) sphingomyelin (c) B3 (d) B5 (c) inhibitor is low (d) substrate is low Phospholipid acting as lung surfactant (a) phosphatidyl inositol 9. (c) glycolysis Which organelle is referred to as the ‘incinerator’ of the cell containing hydrolytic enzymes (a) lysosome 8. (b) citric acid cycle Acute pancreatitis may be diagnosed by the estimation of following enzyme (a) alanine transaminase 7. (d) histidine Uncoupling agent of oxidative phosphorylation is (a) fructose 1,6 bisphosphate 6. (c) serine The hormones that bind to cell surface receptor and requires the second messenger action is (a) thyroxine 5. (d) linolenic acid An amino acid required for porphyrin synthesis is (a) proline 4. (c) arachidonic acid (b) cephalin Pellagra occurs due to deficiency of vitamin (a) B1 (b) B2 10. High Km means enzyme’s affinity for (a) inhibitor is high (b) substrate is high 11. Von Gierke’s disease occurs due to deficiency of the following enzyme (a) muscle glycogen phosphorylase (b) glucose-6-phosphatase (c) debranching enzyme (d) glucose-6-phosphate dehydrogenase 12. Which of the following complexes is inhibited by cyanide (a) complex I (b) complex II (c) complex III (d) complex IV 13. Which of the following mucopolysaccharide acts as a lubricant in joints (a) chondroitin sulfate (b) keratan sulphate (c) dermatan sulphate Downloaded by Aayush Music's (aayushgtbps850@gmail.com) (d) hyaluronic acid lOMoARcPSD|42578826 73 14. Which of the following is a semi essential amino acid (a) lysine (b) arginine (c) tyrosine (d) tryptophan (c) 200-250 mg/dL (d) 250-300 mg/dL (c) copper (d) fluorine 15. Normal range of serum cholesterol is (a) 100-150 mg/dL (b) 150-200 mg/dL 16. Wilson’s disease is due to excess of which mineral (a) cobalt (b) iodine 17. Which of the following enzymes in glycolytic pathway is inhibited by fluoride (a) glyceraldehyde-3-phosphate dehydrogenase (b) phosphoglycerate kinase (c) pyruvate kinase (d) enolase (c) HMG CoA lyase (d) mevalonate kinase (c) 4-5 mg/dL (d) 96-106 mg/dL (c) glucagon (d) thyroxine 18. The regulatory key enzyme of cholesterol synthesis is (a) HMG CoA synthase (b) HMG CoA reductase 19. Reference range of serum calcium is (a) 3-4 mg/dL (b) 9-11 mg/dL 20. Which of the following hormone acts a physiological uncoupler (a) cortisol (b) insulin Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 5 = 50 Enumerate any 5 phospholipids with their biochemical role Mechanisms of enzymes inhibition Describe calcium metabolism Regulation of blood glucose Explain the fluid mosaic model of cell membrane Explain the phase I of xenobiotic metabolism Mucosal block theory of iron absorption Describe fatty liver and add note on lipotropic factors Electron transport chain A two-month-old child was brought to pediatrician with complaints of vomiting after each feed. Mother gave history of failure to gain weight and frequent diarrhea for the last 1 month. On physical examination, child had jaundice and cataract. Benedict’s test was positive and glucose oxidase test was negative (1+1+3) i. What is the most probable inborn error of metabolism ii. Name the enzyme deficient iii. Explain the biochemical basis of jaundice and cataract Q3. Long Answer Questions 3 x 10 = 30 a. A sixty-year-old male was admitted in Medicine ICU with complaints of severe chest pain and profuse sweating. His ECG showed the changes of acute myocardial infarction. Mention different biochemical tests for confirming the above diagnosis along with its reference range. Describe the different diagnostic and prognostic enzymatic markers with their change in concentration with time in the above case. (4+6) b. Describe biosynthesis of urea. How it is regulated, add a note on disorders related to it. (5+2+3) c. A 15-year-old high school boy had difficulty in performing his work in dim light. Dietary history revealed he does not like following foods -carrots, papaya and fish liver oil. I. Based on history and chief complaint the probable diagnosis would be? Name the biochemical compound which is responsible for the above condition. (2) II. Discuss its metabolism under following headings (1+4+3) i. Requirement ii. Functions iii. Deficiency causes and manifestations Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 74 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination April-2022 Time: 3:00 Hours Subject- Biochemistry Paper- II Instructions: a) b) c) d) e) f) Maximum Marks :100 All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. MCQ has to be answered in theory answer book Please write MCQ answer neatly and in serial order with black or blue pen in brackets; for example: - 1. (a) 2. (c) MCQ has to be answered only once, any kind of repetition or cutting or erasing or whitener will be considered as malpractice, such answers will not be counted in marks and action will be taken according to UFM rules of university. Q1. Total MCQs: 20 20 x 1 = 20 1. Rate limiting enzyme in heme synthesis is (a) heme synthase (b) ALA dehydratase 2. (b) obstructive jaundice (c) hemolytic jaundice (d) physiological jaundice (b) transferrin (c) hemoglobin (d) Bence jones protein (b) potassium (c) magnesium (d) calcium (c) starvation (d) cold climate (c) 21-26 mEq/L (d) 30-37 mEq/L Basal metabolic rate is increased by all the following EXCEPT (a) fever (b) thyroxine 7. The normal range of plasma bicarbonate is (a) 5-7 mEq/L 8. (b) 10-15 mEq/L Which of the following hormones affects fluid and electrolyte balance (a) epinephrine 9. (d) 6-8 mmol/dL Major intracellular cation is (a) sodium 6. (c) 3.5-5.5 mmol/dL The first protein to be excreted in urine in glomerular damage is (a) albumin 5. (b) 6-8 gm/dL Conjugated hyperbilirubinemia with increased alkaline phosphatase level is present in (a) viral hepatitis 4. (d) uroporphyrinogen synthase Normal range of albumin is (a) 3.5-5.5 gm/dL 3. (c) ALA synthase (b) glucagon (c) thyroxine (d) aldosterone (c) western blotting (d) none (c) 30% (d) 60% (c) oncogene (d) proximate carcinogen (c) pancreatic function (d) gastric function (c) carbohydrate content (d) electrophoretic ability (c) 14L (d) 3.5L RNA in a mixture can be identified by (a) southern blotting (b) northern blotting 10. Specific dynamic action for protein is (a) 5% (b) 13% 11. Tumour suppressor gene are sometimes called (a) antioncogenes (b) proto-oncogenes 12. Serum urea and creatine are markers of (a) liver function (b) renal function 13. Immunoglobulins are classified on basis of (a) light chains (b) heavy chains 14. In a 70 kg adult, the total body water content is (a) 42L (b) 28L Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 75 15. Which of the following separates DNA like a zip opener (a) DNA ligase (b) DNA polymerase (c) DNA helicase (d) DNA topoisomerase 16. Human genome map where genes are visualized as bands with the help of a chemical dye is (a) cytogenic map (b) gene linkage map (c) restriction fragment length (d) physical map (c) UAG (d) UGA (c) glutathione peroxidase (d) cytochrome p450 monooxygenase 17. All of the following are terminating codons EXCEPT (a) UAA (b) UGG 18. All of the following are anti-oxidant enzymes EXCEPT (a) superoxide dismutase (b) catalase 19. A boy was stung by an unknown insect. He experienced respiratory distress within minutes and lapsed into unconsciousness. This may be mediated by (a) IgG (b) IgE (c) sensitized T cells (d) complement 20. DNA synthesis or extension is the third step of PCR, which is carried out at (a) 95oC (b) 55oC (c) 72oC Q2. Short Answer Questions a. b. c. d. e. f. g. h. i. j. (d) room temperature 10 x 5 = 50 50-year-old male came in medicine OPD with chief complaint of swelling in joint of great toe. On biochemical laboratory investigations, physician noticed the increased serum uric acid. Physician diagnosed him to be suffering with gout. Explain the metabolic pathways steps along with enzymes that is affected in above patient with its biochemical correlation. Explain acid base disorders with their compensatory mechanism Applications of recombinant DNA technology A 60-year-old male is admitted in medicine ICU with complaints of increased micturition and abdominal pain. He was known case of chronic kidney disease (CKD). Describe different tests which can be performed in above patient for the measurement of glomerular filtration rate (GFR). Define replication. Write a note on DNA repair mechanism Free radicals and antioxidants Write the features of primary and secondary dehydration Quality control in clinical laboratory Describe the disorders of heme synthesis Balanced diet and specific dynamic action Q3. Long Answer Questions 3 x 10 = 30 a. A 45-year-old man had acute pain in the right abdomen. He was diagnosed with gall stones i. What are the various types of jaundice? Which type of jaundice do gall stones cause ii. What investigations can be done in the blood and urine to find out the severity and type of jaundice iii. What is the relationship of cholesterol in bile to development of gall stones? Why do bile acids help to prevent development of gall stones b. A 5-year-old child with chief complaints of acute onset of pain both lower limbs and past history of painful episodes of abdomen, upper limb presented to casualty. He is pale and febrile. On examination spleen is palpable and icterus is present. History reveals he is known case of sickle cell disease. i. ii. iii. iv. c. What is the etiopathogenesis of sickle cell disease Explain polymerization of HbS in deoxygenated state Explain electrophoresis findings in sickle cell disease Enlist the complications of sickle cell disease Describe the process of protein synthesis in prokaryotes. How does it differ from protein synthesis in eukaryotes Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 76 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination Feb-2023 Subject- Biochemistry Paper- I Time: 3:00 Hours Maximum Marks :100 Instructions: a) b) c) d) e) f) g) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. MCQ has to be answered in theory answer book Please write MCQ answer neatly and in serial order with black or blue pen in brackets; for example: - 1. (a) 2. (c) MCQ has to be answered only once, any kind of repetition or cutting or erasing or whitener will be considered as malpractice, such answers will not be counted in marks and action will be taken according to UFM rules of university. Subjective answer should be answered in up to 30 words per marks. For example, if a question having 2 marks should answered in up to 60 marks. Q1. Total MCQs: 10 1. Lysosomes are produced by the (a) Nucleus 2. (d) All of the above (c) Fructose and Galactose (d) Lactose and Maltose (b) Niacin (c) Riboflavin (d) Vit. B12 (b) 2-3 mg (c) 3.5-4.5 mg (d) 9-11 mg (c) 20-30 mg/dl (d) 200-220 mg/dl (c) HbM (d) HbS (c) Serotonin (d) Heme (c) Multiple Myeloma (d) All of the above (b) Galactose and Mannose (b) >150 mg/dl Which of the following has protective effect against malaria (a) Hb Bart 9. (c) Sucrase (b) Amylase Normal LDL cholesterol level is (a) <100 mg/dl 8. (d) All of the above RDA of copper for adults is (a) 5-1 mg 7. (c) Receptors (b) Carriers Pellagra is due to deficiency of (a) Thiamine 6. (d) Ribosomes Which of the following is an epimeric pair (a) Glucose and Galactose 5. (c) Golgi apparatus Lactose intolerance is due to deficiency of enzyme (a) Lactase 4. (b) Mitochondria Transmembrane proteins serve as (a) Ion channels 3. 10 x 1 = 10 (b) HbF Glycine is used for synthesis of (a) Thyroxine (b) Niacin 10. Bence-Jones proteins are excreted in the urine of patients of (a) Diabetes Mellitus (b) Nephrotic Syndrome Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 77 Q2. Long Answer Questions 2 x 20 = 40 a. Give on account on how fatty acids are activated and transported into the mitochondria. Describe the reactions of Betaoxidation in the mitochondrial matrix. Add a note on ATP yield from palmitate: b. Case study: -A 57-year-old male was brought to the emergency with a history of 12 hrs of shortness of breath, coughing and tightness in the chest region. The symptoms appeared suddenly. His past medical history is significant for diabetes mellitus and hypertension. He is a known smoker and alcoholic. At the time of admission his BP was 180/100mm of Hg. The laboratory tests showed CKMB as 45 IU/L (Ref Range 5-25 IU/L). 1. 2. 3. 4. Suggest the probable diagnosis define enzymes end its classification. define isoenzyme also discuss different isoenzyme forms of any two relevant enzyme along with their characteristics and clinical significance management Q3. Brief Answer Questions a. b. c. d. e. f. 6 x 05 = 30 Structure of cell-membrane Discuss the synthesis of glucose from non-carbohydrate sources Classify) protein based on tl1cir functions, with suitable example Causes and deficiency manifestations of calcium. Communication skill and Its 1mpor1once in doctor patient relationship. Detoxification by conjugation. Q4. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 2 = 20 Risk factors for Atherosclerosis What is oxidative phosphorylation Biochemical role of oxytocin and ADH. Deficiency of vitamin C. Why Cataract is seen in diabetic patient. Respiratory acidosis Why low levels ofVit31Tlln O 12 is seen in vegetarians Biologically important peptides Disorders of urea cycle Energetics of glycolysis Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 78 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination Dec-2022 Subject- Biochemistry Paper- II Time: 3:00 Hours Maximum Marks :100 Instructions: a) b) c) d) e) f) g) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. MCQ has to be answered in theory answer book Please write MCQ answer neatly and in serial order with black or blue pen in brackets; for example: - 1. (a) 2. (c) MCQ has to be answered only once, any kind of repetition or cutting or erasing or whitener will be considered as malpractice, such answers will not be counted in marks and action will be taken according to UFM rules of university. Subjective answer should be answered in up to 30 words per marks. For example, if a question having 2 marks should answered in up to 60 marks. Q1. Total MCQs: 10 1. Opalescent urine is seen in (a) Porphyria 2. (c) Transcription (d) Reverse transcription (b) Semi Conservative (c) Dispersive (d) None of these (b) 1 Regulatory Gene, 1 Promoter site & 1 Operator (c) 1 Regulatory Gene, 1 Promoter site, 1 Operator & 3 Structural genes (d) 2 Regulatory Gene, I Operator & 3 Structural genes (b) codons for lysine (c) codons for arginine (d) Nonsense codons (b) 4-7 mg% (c) 1-3 mg% (d) 2-4 mg% (c) paper chromatography (d) none of these (c) Vitamin A (d) Vitamin K (c) Nature of work (d) Basal metabolic rate (b) ion exchange chromatography Which of the vitamins listed has NO anti-oxidant property? (a) Vitamin C 10. (b) Replication The purification of enzymes is mostly done by (a) affinity chromatography 9. (d) Uric acid The normal Serum Uric acid is- (a) 8-10 mg% 8. (c) Bile pigment UAA, UAG & UGA are (a) Initiating codons 7. (b) Bile acid In Lac operon we see- (a) 1 Regulatory Gene & 1 P site 6. (d) Creatinuria DNA Replication in Eukaryotes is (a) Conservative 5. (c) Chyluria The flow of Genetic information from DNA to RNA to Protein in living cells is (a) Central dogma 4. (b) Alkaptonuria The end product of catabolism of heme is (a) Bile salt 3. 10 x 1 = 10 (b) Vitamin E Which has no role in calculating calorie requirements? (a) Respiratory quotient (b) Specific Dynamic Action Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 79 Q2. Long Answer Questions 2 x 20 = 40 a. How heme is synthesized in body. Add a note on various Porphyrias. b. A patient with chronic renal failure was found to had the following laboratory findings: - serum creatinine 3 gm/dL, serum sodium 120 mmol/L, serum potassium 5.8 mmol/L, serum bicarbonate 15 mmol/L. i. ii. iii. iv. v. what kind of acid base disturbance is like present in this case how does kidney normally regulate extracellular pH what are additional investigations to be done in this patient explain the basis of abnormal electrolyte values explain anion gap Q3. Brief Answer Questions a. b. c. d. e. f. 6 x 05 = 30 KFT Explain the biochemical tests that will help differentiating the types of jaundice. Importance and applications of recombinant DNA technology. Give an account of water distribution and its balance in the body. Explain regulation of genes by repression with examples. Mutations Q4. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 2 = 20 What is the principle of adsorption chromatography Beer and Lambert law Biochemical alterations in protein energy malnutrition Wobble hypothesis Gene therapy Molecular basis of thalassemia and cystic fibrosis Functions of nucleotides Tumour markers Name the pre renal conditions that cause increased blood urea Give two examples of purine analogues used as anticancer drugs Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 80 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination Nov-2023 Subject- Biochemistry Paper- I Time: 3:00 Hours Maximum Marks :100 Instructions: a) b) c) d) e) f) g) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. MCQ has to be answered in theory answer book Please write MCQ answer neatly and in serial order with black or blue pen in brackets; for example: - 1. (a) 2. (c) MCQ has to be answered only once, any kind of repetition or cutting or erasing or whitener will be considered as malpractice, such answers will not be counted in marks and action will be taken according to UFM rules of university. Subjective answer should be answered in up to 30 words per marks. For example, if a question having 2 marks should answered in up to 60 marks. Q1. Total MCQs: 10 1. A homopolysaccharide among the following is (a) heparin 2. (c) threonine (d) proline (b) gastrointestinal disturbances (c) liver ailments (d) nephritis (c) vit. B12 (d) vit. C During respiratory burst, all of the following reactive oxygen species (ROS) are formed, EXCEPT (b) hydrogen peroxide (c) nitric oxide (d) hydroxyl radical (c) xeroderma pigmentosum (d) menke’s disease (c) pompe’s disease (d) leber’s neuropathy (c) kidneys (d) intestinal mucosa (c) iron (d) none of the above Copper deficiency occurs commonly in (b) wilson’s disease Which of the disease listed below is a lysosomal storage disease (a) Zellweger syndrome 9. (d) inability to see in dim light (b) vit. B6 (a) acrodermatitis enteropathy 8. (c) blindness Vegetarians, who do not consume milk should take daily (a) superoxide 7. (b) destruction of cornea The most important clinical importance of the BMR is that it is an indicator of (a) vit. B1 6. (d) cellulose (b) glycine (a) thyroid dysfunction 5. (c) dermatan sulfate Which is the most common amino acid present in collagen (a) lysine 4. (b) hyaluronic acid Nyctalopia is (a) drying of eyes 3. 10 x 1 = 10 (b) inclusion cell bodies 25-hydroxylation of vitamin D occurs in (a) skin (b) liver 10. Vitamin C enhances intestinal absorption of (a) potassium (b) iodine Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 81 Q2. Long Answer Questions 2 x 20 = 40 a. Define enzymes with classification. Write different types of enzymes inhibition with suitable examples. Explain isoenzymes with examples. Indicate the importance of enzyme studies in myocardial infarction. b. A 50-year-old obese lady complains of excessive thirst and hunger, with increased urination. On physical examination she appeared normal. Her laboratory report showed the following findings; random plasma glucose level: 250 mg/dL. Urine was positive for glucose with glucose strips. Her fasting and postprandial plasma glucose levels were 130 mg/dL and 195 mg/dL respectively i. ii. iii. iv. What is your diagnosis and write the biochemical basis of the disorder Which biochemical investigations will help in diagnosis of the disease What are the characteristic clinical features of the disorder Mechanism of action of responsible hormone Q3. Brief Answer Questions a. b. c. d. e. f. 6 x 05 = 30 What does it mean to be a doctor Regulation of plasma calcium level One year old female child with low socio-economic status comes to OPD with retarded growth and emaciated appearance. No edema is present i. Identify the condition ii. What is the difference between marasmus and kwashiorkor iii. What is the cause of emaciated appearance Explain cholesterol metabolism Describe electron transport chain. Write uncouplers with their significance Write the sources, RDA, and deficiency manifestations of vitamin A Q4. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 2 = 20 Enumerate biologically important peptides Enumerate antioxidant enzymes and minerals Nitrogen balance Biochemical importance of serotonin Alkaptonuria Well labeled diagram of cell membrane Define classify carbohydrates Define detoxification with its examples Enumerate essential fatty acids Wilson’s disease manifestations Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 82 Madhya Pradesh Medical Science University, Jabalpur MBBS First Professional Examination Nov-2023 Subject- Biochemistry Paper- II Time: 3:00 Hours Maximum Marks :100 Instructions: a) b) c) d) e) f) g) All questions are compulsory Draw diagrams wherever necessary Answers of Questions and Sub-questions must be written strictly according to serial order of question paper. MCQ has to be answered in theory answer book Please write MCQ answer neatly and in serial order with black or blue pen in brackets; for example: - 1. (a) 2. (c) MCQ has to be answered only once, any kind of repetition or cutting or erasing or whitener will be considered as malpractice, such answers will not be counted in marks and action will be taken according to UFM rules of university. Subjective answer should be answered in up to 30 words per marks. For example, if a question having 2 marks should answered in up to 60 marks. Q1. Total MCQs: 10 1. An amino acid required for porphyrin synthesis is (a) proline 2. (c) IgM (d) IgD (b) 8-12 mEq/L (c) 20-25 mEq/L (d) 35-40 mEq/L (b) mmol/day (c) mg/min (d) mL/min (b) methotrexate (c) azidodeoxythymidine (d) 5-fluorouracil (c) carcinoma of pancreas (d) stone in gall bladder (c) phosphate buffer (d) bicarbonate buffer (c) DNA (d) glycosaminoglycans (c) alpha 2-macroglobulin (d) c- reactive protein Unconjugated bilirubin is raised mostly in (b) obstructive jaundice Most important physiological buffer in the blood is (a) hemoglobin buffer system 9. (d) yeast plasmid Which of the following chemotherapeutic drugs works by inhibiting de novo purine synthesis (a) hemolytic jaundice 8. (c) hybrid of plasmid and phage Unit of clearance is (a) acyclovir 7. (b) viral plasmid Normal anion gap in plasma is around (a) mg/dL 6. (d) histidine (b) IgG (a) 1-5 mEq/L 5. (c) serine The most abundant immunoglobin present in plasma is (a) IgA 4. (b) glycine A cosmid is a (a) large bacterial plasmid 3. 10 x 1 = 10 (b) acetate buffer The main biological targets for attack by free radical are all EXCEPT (a) PUFA (b) protein 10. Cigarette smoke inhibits the activity of (a) alpha 1-antitrypsin (b) ceruloplasmin Downloaded by Aayush Music's (aayushgtbps850@gmail.com) lOMoARcPSD|42578826 83 Q2. Long Answer Questions 2 x 20 = 40 a. A 4 years old boy suffered from pain in joints, hypotonia, developmental delay, irritability, showed signs of mental retardation and evidences of self-mutilation. On examination there was testicular atrophy and hematuria. The serum uric acid level was 10 mg/dL i. What is the probable diagnosis ii. Which enzyme is defective in this disease iii. What is the normal level of serum uric acid in humans iv. What is the mode of inheritance of this disease v. What is the biochemical basis of this condition b. Classify liver function tests according to various functions of liver. Write enzymatic, non-enzymatic and urinary markers based on type of jaundice Q3. Brief Answer Questions a. b. c. d. e. f. 6 x 05 = 30 Explain metabolic acidosis and its compensation Describe post translational modifications and inhibitors of this process Explain kidney function tests Explain recombinant DNA technology and its applications Explain eukaryotic rRNA processing Explain the mechanism of free radical formation and describe different antioxidants with their mechanisms Q4. Short Answer Questions a. b. c. d. e. f. g. h. i. j. 10 x 2 = 20 Enumerate tumour markers Enumerate cause of overhydration Types of RNA and their functions Enumerate derivatives of hemoglobin Write types of gout and their biochemical causes Uses of quality control in biochemistry lab Enumerate disorders due to changes in immunoglobulins Enumerate oncogenes Write various types of plasma proteins Write any two types of mutations with examples Downloaded by Aayush Music's (aayushgtbps850@gmail.com)
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