Chapter 1 Plant and Cell Architecture Figures to know • 1.2. • 1.3. • 1.5. • 1.6. • 1.11. • 1.23. • 1.25. • 1.27. Concepts to know. • Describe unique features of plant life cycles. Or plant life processes. ▪ Plants alternate between two distinct multicellular generations to complete their life cycle – One generation has diploid cells, cells with two copies of each chromosome, 2N - sporophyte – One generation has haploid cells, cells with only one copy of each chromosome, 1N gametophyte • • • • ▪ Monoecious – both male and female parts ▪ Diecious – individual sexes per plant Be able to describe the plant life cycle that alternates between diploid and haploid generations. ▪ One generation has diploid cells, cells with two copies of each chromosome, 2N – sporophyte ▪ One generation has haploid cells, cells with only one copy of each chromosome, 1N gametophyte Describe the fundamental properties of plant structures, including cell walls. ▪ The vegetative body consists of three organs: 1. Stem - grows upward and supports the above ground part of the plant 2. Root - anchors the plant and absorbs nutrients and water, and grows down below the ground 3. Leaves - primary function is photosynthesis, grow laterally from the stems at the node ▪ 2 types of cell wall 1. Primary cell walls – Typically <1 micrometer thin – new growth 2. Secondary wall - Thicker and stronger than primary wall a. Deposited on the inner surface of the primary wall after most cells’ enlargements has ended b. Owe their strength and toughness to lignin, a brittle material Distinguish the structural and functional difference of plant tissue types. 1. Dermal tissue - forms the outer protective layer of the plant, epidermis in the primary plant body 2. Ground tissue - fills out the 3D bulk of the plant and includes the pith and cortex of primary stems and roots and the mesophyll in leaves pith, cortex, stem, roots, and mesophyll 3. Vascular tissue – consists of two types of tissues: xylem and phloem, each of the which consists of conducting cells, generalized parenchyma cells and thick-walled fibers Be able to explain the biochemical structure and behavior of plant cell membranes and associated proteins. ▪ The endomembrane system and peroxisomes: a. Except for some peroxisomes components of the endomembrane system are not formed by semiautonomous processes b. The endomembrane system plays a central role in secretory processes, cell signaling, specialized metabolite and hormone production, membrane recycling, the cell cycle and cell expansion. cell secretion, signaling, metabolite, hormone, cycle, and expansion 2. Semiautonomous (independently dividing) organelles of endosymbiotic origin: a. Plastids and mitochondria, these organelles function in energy metabolism and storage, and they synthesize a wide range of metabolites in the biosynthesis of all cell components. oxygen and glucose ▪ Biological Membranes: 1. Integral membrane proteins- proteins that are embedded in the lipid bilayer of a membrane via at least one transmembrane domain. • • • • 2. Peripheral membrane proteins- proteins that are bound to the membrane surface by noncovalent bonds, such as ionic bonds, or hydrogen bonds. 3. Anchored proteins – proteins that are bound to the membrane surface via lipid molecules, to which they are covalently attached. 4. Glycosylphosphatidylinositol anchor – in plants, a phosphoglyceride protein modification that anchors proteins to ordered plasma membrane domains. Define the elements of the cytoskeleton and explain their biological activity. ▪ Microtubules: a. Are polarized hollow cylinders with an outer diameter of 25 nm; composed of polymers of the protein tubulin b. a component of the cell cytoskeleton and the mitotic spindles, and a player in the orientation of cellulose microfibrils in the cell wall. Made of tubulin 2. Microfilaments: a. Are a solid with a diameter of 7nm; composed of the monomeric form of the protein Actin, or globular actin (G-actin). b. A component of the cell cytoskeleton made of an Actin; it is involved in organelle motility within cells. Describe the role of the endomembrane system components in the left cells ▪ Exocytosis - the process that allows forward trafficking of biosynthetic cargo and membranes from the site of synthesis, the ER and the Golgi apparatus ▪ Endocytosis - is the opposite (retrograde) process of exocytosis. The plasma membrane forms invaginations that allow the cell to incorporate internalized plasma membrane and extracellular material into the cell or recycle those components back to the plasma membrane ▪ Autophagy - a catabolic mechanism that conveys cellular macromolecules and organelles via autophagosomes to lytic vacuoles where they are degraded and recycled Differentiate between the two main types of semi-autonomous organelles in plants ▪ Mitochondria - Specialize in cellular respiration to produce ATP by breaking down glucose and oxygen ▪ Matrix - enzymes, ribosomes, and circular DNA ▪ Main location of the enzymes involved in oxidative phosphorylation and energy metabolism ▪ Divided by fission ▪ Plastids - Specialize in photosynthesis to produce glucose and oxygen using light energy ▪ Specialize in photosynthesis to produce glucose and oxygen using light energy ▪ In addition to their inner and outer envelope membranes, chloroplasts possess a third system of membranes called thylakoids ▪ The fluid compartment surrounding the thylakoids, called the stroma, is analogous to the matrix of the mitochondrion Distinguish between the different molecular and cellular processes into their corresponding phase in the cell cycle. ▪ Stages of mitosis: 1. Prophase 2. Metaphase 3. Anaphase 4. Telophase 5. Cytokinesis 6. Cell plate Chapter 2 Water and Plant Cells Vocabulary • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Adhesion - the attraction of water to a solid phase such as a cell wall or glass surface, due primarily to the formation of hydrogen bonds Aquaporins - facilitate the movement of water across membranes Capillarity - the movement of water for small distances up a glass capillary tube or within the cell wall, due to water’s cohesion, adhesion, and surface tension Cavitation - the collapse of tension in a column of water resulting from the formation and expansion of tiny gas bubbles Chemical potential - quantitative expression of the free energy associated with a substance Cohesion - mutual attraction between water molecules due to extensive hydrogen bonding Contact angle - quantitative measure of the degree to which a water molecule is attracted to a solid phase versus to itself Diffusion - the net movement of substances form regions of higher to lower concentration Electronegative - The tendency of an atom in a molecule to attract the shared pair of electrons towards itself Gravitational potential - depends on the height (h) of the water above the reference-state water, the density of water and the acceleration due to gravity Halophytes - plants that are native to saline soils and complete their life cycles in that environment Hydraulic conductivity - the ease with which a fluid (usually water) can move through the pore space, or fracture network Hydrogen bonds - an attractive dipole-dipole interaction between a partially positive charged hydrogen atom in one molecule and a partially negative charged atom in the same or different molecule Hydrostatic pressure - the pressure generated by compression of water into a confined space Latent heat of vaporization - the energy needed to separate molecules form the liquid phase and move them into the gas phase at Constant temperature Matrix potential - adhesion to structures such as cell walls, membranes, and soil particles Osmosis - the net movement of a solvent across a selectively permeable barrier from a region of lower to higher solute concentration Osmotic potential - effect that solutes have on ψ Polar – molecule with uneven distribution of electrical charge Pressure potential - effect that pressure has on ψ Selectively permeable - the ability of a membrane to allow certain molecules or ions to pass through it while inhibiting the passage of others Solute potential - the effect of dissolves solutes on water potential Specific heat capacity - The Ratio of the heat capacity of a substance to the heat capacity of a reference substance Surface tension - The energy required to increase the surface area of a gas-liquid interface Tensile strength - the maximum force per unit area that a continuous column of water can withstand before breaking Tension - Negative hydrostatic pressures frequently develop in xylem conduits Transpiration - the evaporation of water from the surface of leaves and stems Turgor pressure - Positive hydrostatic pressure in cells Water potential - The measure of the relative tendency of water to move from one area to another Figures to know. • 2.3. • 2.7. Concepts to know: • • • • • Understand the role of water as a limiting resource for natural and agricultural ecosystems ▪ underscores the need for sustainable management practices to ensure the well-being of both natural and agricultural ecosystems ▪ most abundant and at the same time the most limiting for productivity Describe the physical and chemical properties that make water an excellent solvent. ▪ medium for the movement of molecules within and between cells and greatly influences the structure of proteins, nucleic acids, polysaccharides, and other cell constituents Explain the importance of diffusion and osmosis in transport over short cellular distances. ▪ Diffusion is the movement of molecules or ions from an area of higher concentration to an area of lower concentration ▪ Osmosis is the movement of water molecules across a selectively permeable membrane from an area of lower solute concentration to an area of higher solute concentration ▪ Fundamental processes that enable cells to maintain their internal environment, support metabolic activities, and communicate with their surroundings Know the water potential formula and describe how each of the three main factors contribute to it. ▪ ψ = ψπ + ψp + ψm ▪ ψp = pressure potential: effect that pressure has on ψ– If water is under pressure, both pressure potential and water potential increase. ▪ ψπ = osmotic potential: effect that solutes have on ψ– Adding solutes decreases water’s free energy, so ψπ is always negative. ▪ ψm = matric potential: adhesion to structures such as cell walls, membranes, and soil particles Describe the movement of water at the cellular level under different values of water potential. Explain the different physiological functions of cell wall and cell membrane with respect to water movement and plants. ▪ • The movement of water at the cellular level is crucial for maintaining cellular functions and overall plant health. The cell wall and cell membrane each play vital roles in regulating this movement and ensuring proper physiological responses Describe how water status affects many other functions in plants. ▪ Water status is a fundamental factor that affects almost every aspect of plant life. Proper water management is crucial for ensuring optimal growth, productivity, and resilience in plants Chapter 3 Water Balance of Plants Vocabulary. • • • • • • • • • • • • • • • • • Casparian strip - band within the radial cell walls of the endodermis that is impregnated with lignin Cellulose microfibrils - elongated structures in plant cell walls composed of cellulose chains arranged in a highly organized, crystalline fashion. Provide exceptional mechanical strength, contributing to the stiffness and solidity of plant tissues Chloroplast envelope - a double membrane system surrounding the chloroplast Difference in water vapor concentration - between leaf and air are responsible for the diffusion of water vapor from the leaf to the air Diffusional Resistance - the restriction posed by the boundary layer and the stomata to the free diffusion of gases from and into the leaf Guard cells - surround the stomatal pore. Have specific shapes for specific jobs and portions of the walls are substantially thick for protection. Leaf stomatal resistance - The resistance associated with the diffusion through stomatal pores - The resistance of CO2 diffusion imposed by the stomatal pores. Lignin - highly branched phenolic polymer made-up of phenylpropanoid alcohols that is deposited in secondary cell walls Microfibrils - a small fibril in the cytoplasm or wall of a cell, visible only under an electron microscope, and typically aggregated into coarser fibrils or structures Osmotic potential - The effect of dissolved solutes on water potential, also called solute potential Soil hydraulic conductivity - a measure of the ease with which water moves through soil Stomatal complex - crucial structure in plant biology that plays a vital role in regulating gas exchange and water loss. It consists of the stomata (tiny openings or pores) and the surrounding specialized cells, mainly the guard cells and sometimes subsidiary cells Subsidiary cells - pair of differentiated epidermal cells called subsidiary cells – help control stomatal pore Tracheids - elongated spindle shaped cells that are arranged in overlapping vertical files Transpiration ratio - measures the relationship between water loss and carbon gain Vessels - one of the two main types of xylem elements, the other being Tracheids. They play a crucial role in the transport of water and dissolve minerals from the roots to the rest of the plant Vessel elements - end walls that are partially or completely open, so they can be stacked end to end to form a multicellular conduit called a vessel Figures to know • 3.1. • 3.2. • 3.4. • 3.10. • 3.12 Concepts to know. • • • • • Explain the forces involved in water movement through the soil. ▪ Gravity – responsible for the drainage of excess water from the soil, especially after heavy rainfall or irrigation ▪ Osmotic potential – Water moves from areas of lower solute concentration to areas of higher solute concentration ▪ Capillary action – helps in the upward movement of water from wetter to drier soil layers, particularly in the absence of rainfall ▪ Matric potential – determines the availability of water to plants by holding water in the soil pores ▪ Hydraulic conductivity – influenced by soil texture, structure, and moisture content. Soils with high hydraulic conductivity, like sandy soils, allow water to move more freely compared to clayey soils Explain how water is absorbed by roots and how it reaches the Xylem. ▪ Contact between the surface of the root and the soil ▪ Root hairs are a filamentous outgrowths of root epidermal cells – expand area of root ▪ Water flows between soil particles from the epidermis to the endodermis of the root Describe the role of xylem in water transport and the forces involved in water movement through these cells. ▪ Contributes to root pressure in plant ▪ 99.5% of the water transport pathway through the plant is within the xylem Explain how water moves from the leaf to the atmosphere and how the stomata regulate this process. ▪ When leaves open their stomata to obtain CO2 for photosynthesis, water vapor diffuses out of the leaves causing water to evaporate from the surface of the mesophyll cell walls inside the leaves - diffusion Differentiate the mechanisms of water transport from the soil through the plant to the atmosphere ▪ 3 mechanisms of transport: 1. In the soil and the Xylem, liquid water moves by bulk flow in response to a pressure gradient. 2. When liquid water is transported into and out of cells, the driving force is the water potential difference across the plasma membrane. 3. In the vapor phase, water moves primarily by diffusion, at least until it reaches the outside air, where convection, a form of bulk flow, becomes dominant.
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