EVALUACIÓN PARCIAL 3 BIOINFORMÁTICA
“ALINEAMIENTOS”
NOMBRE COMPLETO: Teresita de Jesús Grajeda Blanco
INDICACIONES: Localice las secuencias que le fueron asignadas.
Utilice los ID de secuencias que le fueron proporcionadas para llevar a cabo las siguientes
actividades:
ACTIVIDAD 1: Utilizando los primeros dos ID de secuencias, realiza un alineamiento de
pares con la herramienta BLASTp. (20 pts).
Coloca una captura de pantalla de tu alineamiento en BLAST aquí…
Tomando como base los datos obtenidos contesta la siguiente tabla:
Nombre de la PROTEÍNA asignada: _____________________
Alineamiento
Máximo
(organismos)
puntaje
Saccharomyces 563
kudriavzevii
IFO 1802
vs
Saccharomyces
cerevisiae
YJM1439
___________
Puntaje
total
563
Cubertura
Valor E
99%
0.0
%
Identidad
58.89
ACTIVIDAD 2: Utilizando las mismas secuencias con las que trabajaste en la actividad 1,
realiza un alineamiento de pares utilizando la herramienta LALING. (20 pts).
Coloca una captura de pantalla de tu alineamiento en LALING aquí:
>>>EJT44508.1, 451 aa vs lalign-I20250409-193021-0973-74025452-p1m.bsequence
library
>>AJW08323.1 Eeb1p [Saccharomyces cerevisiae YJM1439]
(456 aa)
Waterman-Eggert score: 1851; 500.9 bits; E(1) < 3.3e-146
58.3% identity (83.6% similar) in 456 aa overlap (1-451:1-456)
10
20
30
40
50
60
EJT445 MSEVSKWPAINPFHWGYNGTVSHVVGENGSMKLGLKDNDDQVEFDEFVNKYVPILKNGAH
: . . .:...: ::::::::.::.::.:. .:...:. :. . :::.:.:: ::.::.
AJW083 MFRSGYYPTVTPSHWGYNGTVKHVLGEKGTKSLAFRDSKRQIPLHEFVTKHVPTLKDGAN
10
20
30
40
50
60
70
80
90
100
110
120
EJT445 FNLSPYLFTGILQTLYLNAADFSKKFPVFYGREIVKFSDNGVCTADWVMNSWKKEYKFDQ
: :. :::: ::::::.:.:::::: :::::::.::::.::::::::: :.. :...
AJW083 FRLNSLLFTGYLQTLYLSAGDFSKKFQVFYGREIIKFSDGGVCTADWVMPEWEQTYSLNA
70
80
90
100
110
120
130
140
150
160
170
EJT445 ITRKFDTKKFGNDEKATHPEGWPRLQPRTRYLRDDELKDLGE--VDLPLVVILHGLAGGS
.:. :.:.::::::::.:::::.:::::: ..::.
. ::::.::::::::
AJW083 EKASFNEKQFSNDEKATHPKGWPRLHPRTRYLSSEELEKCHSKGYSYPLVVVLHGLAGGS
130
140
150
160
170
180
180
190
200
210
220
230
EJT445 HEPIIRSLAEGLSR--DGRFEVVILNTRGCARSKITTKILFTAYHTIDLREFLQREKQRH
:::.::.:.: ::. ::.:.::.::.:::.:::.::. .::: :: :.::::...:
AJW083 HEPLIRALSEDLSKVGDGKFQVVVLNARGCSRSKVTTRRIFTALHTGDVREFLNHQKALF
190
200
210
220
230
240
240
250
260
270
280
290
EJT445 PNRKLYAVGCSFGATMLGNYLGEEGDKSPLSAAVTLCNPWDLLLSAVRMNEDWWSKTLFS
:.::.:::: ::::.:: ::::::::. ::.:::.: ::::.. . .. .::::. .::
AJW083 PQRKIYAVGTSFGAAMLTNYLGEEGDNCPLKAAVALSNPWDFVHTWDKLAHDWWSNHIFS
250
260
270
280
290
300
300
310
320
330
340
350
EJT445 KNIAQFLTRTVQVNMGELGVPNGSRPDHPPTVKNPSYYMFTPDNLVKAKRFKSSLEFDEV
....:::::::.:::.:: ::.. . .: :::..: .: .: .:: ::..: . ::::..
AJW083 RTLTQFLTRTVKVNMNELQVPENFEVSHKPTVEKPVFYTYTRENLEKAEKFTDILEFDNL
310
320
330
340
350
360
360
370
380
390
400
410
EJT445 FTAPALGFPNAMEYYNAASSINRVDTIRVPTLVINSRDDPVVGPDQ-PYSIVEKNPRILY
::::..:.:... :: ::::::. .:..:::.::. ::::.: . ::. ...:: .:
AJW083 FTAPSMGLPDGLTYYRKASSINRLPNIKIPTLIINATDDPVTGENVIPYKQARENPCVLL
370
380
390
400
410
420
420
430
440
450
EJT445 CRTDLGGHLAYLDNDNNSWATKAIAEFLTKFDELVV
:.::::::::::::.:::: :: :::: .:::::.
AJW083 CETDLGGHLAYLDNENNSWLTKQAAEFLGSFDELVL
430
440
450
Tomando como base los datos obtenidos contesta la siguiente tabla:
Alineamiento
(organismos)
WatermanEggert
score
Saccharomyces 1851
kudriavzevii
IFO 1802
vs
Saccharomyces
cerevisiae
YJM1439
Valor E
Identidad Similitud
Cobertura
< 3.3e146
58.3 %
1-451:1-456
83.6%
ALINEAMIENTOS MÚLTIPLES:
En esta sección deberás realizar un alineamiento múltiple con todos los ID de las secuencias.
Realiza la búsqueda de tus secuencias y pídelas en formato FASTA text.
Edita el identificador FASTA (Sólo mantén el signo >, el identificador y las iniciales de tu
organismo unidas por un -, no dejes espacios) Ejemplo: >NP_013287.1-Sc
OJO: Guarda las secuencias en un block de notas de deberás de subir junto a tu examen
nombrado de la siguiente forma: PROTEINA-NOMBRE (Ejemplo: Actina-LauraIniguez).
Además, coloca tus secuencias editadas a continuación (5 pts):
ACTIVIDAD 3: Utiliza la herramienta CLUSTAL OMEGA para realizar el alineamiento
múltiple de tus secuencias. Coloca las capturas de pantalla aquí: (15 pts)
CLUSTAL O(1.2.4) multiple sequence alignment
KKA03693.1-Hu
-----MSALILPEEKNFSS----TPVEKASKITDKLPILTPWKWSYTGKVSQIHSNTKT
50
BAO38417.1-Km
----------------------------------MVFLFNPAHWGYNGTITQHIGQEGT
25
XP_455042.1-Kl
---------------------------------MVNLLFNPLHWGYNGTVTQHIGEKGT
26
CDH12365.1-Zb
--------------------------------MSGLPIINPLHWGFNGTVKQTYDPSGT
27
XP_002497841.1-Zr
--------------------------------MSNLPIINPFHWGSRGTLKHTSAPSGT
27
AJW08323.1-Sc
-----------------------------MFRSGYYPTVTPSHWGYNGTVKHVLGEKGT 30
EHM99903.1-Sc
-----------------------------MFRSGYYPTITPSHWGYNGTVKHVLGEKGT 30
EJT44508.1-Sk
-----------------------------MSEVSKWPAINPFHWGYNGTVSHVVGENGS 30
XP_002554056.1-Lt
---------------------------------MPLPIFNPFHWGYHGTIEQVSNPNGT
26
KGK36953.1-Pk
---------------------------------------MLPNWGFRNTVHSYHAENP19
XP_002490078.1-Kp
----------------------------------------MPSWGFHNQIHATYSDKS18
XP_002550505.1-Ct
MKKSKLVPCLISSSFTFTSSSSSSSGISFIACFLHNTKVMTVLDWGYRANIKVHQAGDD59
XP_001385510.2-St
--------------------------------------MGILNWGFRANVKVHQSSKA20
*.
KKA03693.1-Hu
VTLKDGS------NKTVKLHEFIDENVPAFKDNATFSLKPTYFTGMLQTLALSDPSLNK 103
BAO38417.1-Km
VKLQKKDG------TEEQLHELISKEVPNLADGAKFQLHPMLFTGILQTMYLAAGDFSK 78
:
XP_455042.1-Kl
IKLQKKDG------SEAQLHELISEEVTGLSDGSKFQLSPLIFTGILQTMYLAGGDFSK 79
CDH12365.1-Zb
TRLTMRRT-----KDKIEFRHFVNQYVPTLKDGHSFRLHHFLFTGILQTMYLSTADFSK 81
XP_002497841.1-Zr
TKLTLNHD-----KTKIDFQHFVSQYVPALKDGSKFKLNNFLFTGILQTMYLSGADYTK 81
AJW08323.1-Sc
KSLAFRDS-----KRQIPLHEFVTKHVPTLKDGANFRLNSLLFTGYLQTLYLSAGDFSK 84
EHM99903.1-Sc
KTLAFKGS-----KNQVPLHEFVTKHVPTLKDGANFRLNSLLFTGYLQTLYLSGGDFSK 84
EJT44508.1-Sk
MKLGLKDN-----DDQVEFDEFVNKYVPILKNGAHFNLSPYLFTGILQTLYLNAADFSK 84
XP_002554056.1-Lt
VALTLKDE-----KKPVQFSDFVSREIPGLKDKAKFEVNPLLFTGYLQTLYLGGADFSK 80
KGK36953.1-Pk
IELPLKSSNDCASGNSVVSLDKFVEKNIPEIKDGARHYLKPTLFTGTLQTLHTFKGDFHT
79
XP_002490078.1-Kp
VALKLKSGKNDFAPLNVIPFSTFIKTLVPELSDGYKFNLHPFLFNGALQTLYASKADYSK
78
XP_002550505.1-Ct
HSANLKLKDN---NGTVKFSQFIKQDLKFLDPSKKLYLNPYLFNGSLQTLYYTSMDSSN 115
XP_001385510.2-St
YDLPLKS-NK---EDSISFTDFVRKEIPTLDESKKLWLHPLLFNGALQTLYYTSVDSIA 75
: ::
: :
:
*.* ***:
.
KKA03693.1-Hu
FFKIFYGREVFTYK-----D--------GGVCSVDYVMNNNKGWKNKYSFEFLKNGKIS 149
BAO38417.1-Km
KFNVFYGRELFELS-----D---------SGIASVDWVRN--DWKKDYDFDPA----TG
117
XP_455042.1-Kl
KFNVFYGRELFEMS-----D---------HGIASIDWVRN--DWKKLYELDPA----TG
118
CDH12365.1-Zb
CFPVFYGREIVEYS-----D---------GGASTTDWVMK--SWEKKYELDI-----YG
119
XP_002497841.1-Zr
WFPVFYGREILELS-----D---------GGVCTVDNVMV--SWEEKYQLRQN----SG
120
AJW08323.1-Sc
KFQVFYGREIIKFS-----D---------GGVCTADWVMP--EWEQTYSLNAE----KA
123
EHM99903.1-Sc
KFQVFYGREIVKFT-----D---------GGVCTADWVMP--EWEEKYSFNGK----RA
123
EJT44508.1-Sk
KFPVFYGREIVKFS-----D---------NGVCTADWVMN--SWKKEYKFDQI----TR
123
XP_002554056.1-Lt
SFPVYYGREIVKFS-----D---------GGICTADWVMK---SWKSKYGAD------- 115
KGK36953.1-Pk
KYPVYFAREIVSLSHEEAKELKGTFKHIHAGEFTLDYLVNPP---------------SE 123
XP_002490078.1-Kp
EFQIYYGRCMVRYP-----EANHEYPHLHLGQATADFVVDPP---------------TE 117
XP_002550505.1-Ct
KFMIYYGRELFTYK-----D---------NGICSLDWVIPKP---------------KN 145
XP_001385510.2-St
KFPVYYGRETFEYK-----D---------QGTCSLDWAIPKP---------------ES 105
: :::.* .
:
* : *
KKA03693.1-Hu
GFDKDTFKKDEESTHLQDWPRLHPRTKYLTTEELEEV--K--ASKTKPLVLICHGLCGG
204
BAO38417.1-Km
SYNKQKLAEDGAKTHPEGWPRLHPRTRYMTEDEKKGL--L--DDTSKPLIIVMHGLAGG
172
XP_455042.1-Kl
TYNKEKLAEDGKKTHPEGWPRLHPRTRYMDDEEKESV--L--NDTSKPLIIVMHGLAGG
173
CDH12365.1-Zb
NYKKLEFERDEEATHPDNWPRLHPRTRYFSEEEKSAV--H--A-DERPLIVIIHGLAGG 173
XP_002497841.1-Zr
SFNKLEFEKDEKDTHPQNWPRLQARTRYLTAKELAEV--H--G-DQRPLVVVLHGLAGG 174
AJW08323.1-Sc
SFNEKQFSNDEKATHPKGWPRLHPRTRYLSSEELEKC--H-SKGYSYPLVVVLHGLAGG
179
EHM99903.1-Sc
TYNEKLFSNDEKATHPKDWPRLHPRTRYLSSKELQRC--H-SAGDSYPLVVILHGLAGG
179
EJT44508.1-Sk
KFDTKKFGNDEKATHPEGWPRLQPRTRYLRDDELKDL--G--E-VDLPLVVILHGLAGG 177
XP_002554056.1-Lt
---TSSFKTDEQATHPENWPRLHPRTRFLEESEKKDV--H--N-SEKPLVVVLHGLAGG 166
KGK36953.1-Pk
ELNDEYFSRKCKETMPEGYPRLHPRCRYYAPNELAQL-KNEWKQDNSPISIILPGLAGG
181
XP_002490078.1-Kp
I---DEWNYLYNQTLPEGWPRLHPRTRFLTVQEEQDL-EADWASNSGSIVVIIHGLAGG
172
XP_002550505.1-Ct
P---EQFKRLQKETMPEGFPRLHPRCRYFDKEELSQL-HEENLENTKPVVAILHGLAGG
200
XP_001385510.2-St
K--EEFKKLHDETLPEDSPKLHPRTRFFSEQELAERKKHGQDVGSEKPIAVVLHGLCGG
162
* .. *:*: * ::
.*
**.**
KKA03693.1-Hu
SHETIIRATI--DKIDLDVFDVAVINSRGCARTKIMSEKLFSAYSTDDIREIVTAKLND 261
BAO38417.1-Km
SHEPIIRSLTEQLSTISDEKFQVAVLNTRGCCRTKVVSHKLFYAFATEDLRELVQREHKR
XP_455042.1-Kl
SHEPIIRSLTEQLSNISNEKFKVAVLNTRGCARTKVVSPKLFYAFATEDLREFVQREHKR
CDH12365.1-Zb
SHEPMIRSLTAKLSTIAGERFQVVVLNCRGCARSKITNKKLFSAFQTGDLKEFLAREKAR
XP_002497841.1-Zr
SHETIIRSLTSKLSKIDGGKFQVAVLNCRGCARSKITNKKLFSAFQTGDLKEYLAREKSR
AJW08323.1-Sc
SHEPLIRALSEDLSKVGDGKFQVVVLNARGCSRSKVTTRRIFTALHTGDVREFLNHQKAL
EHM99903.1-Sc
SHEPLIRALSEDLSRVGDGKFQVVVLNARGCSRSKVTTRRIFTALHTGDVREFLNHQRAL
EJT44508.1-Sk
SHEPIIRSLAEGLSR-DGRFEVVILNTRGCARSKITTKILFTAYHTIDLREFLQREKQR
235
XP_002554056.1-Lt
SHEPIIRSLTQDLSNAGDSKFDVVVLNCRGCARSKITTRKLFYAVFTSDIREFIAREKAR
KGK36953.1-Pk
IQEAPIRATCYKL--QQKGHRVVVLNQRGCSRSKITTPHMFTGLDTDDLKYILHKFHDE
238
XP_002490078.1-Kp
SHEPGIRDVSQHL--HQAGFNVVTLNSRGCCRSKLSTGRLYSAVETDDLRYFIDELHKK
229
232
233
233
234
239
239
226
:
:
XP_002550505.1-Ct
SHESLIRNFAEQINKNTKDWDVVVINSRGCARTKITNGKLFTAGSTDDIREVLQTFKQR
XP_001385510.2-St
SHEPLIRNFVETINSHVNGWDVLVVNNRGCCRTKITSAKLFNALSTDDVKDVLHELKKR
:* **
:
259
*
221
:* ***.*:*: .
KKA03693.1-Hu
NP----DRHIYLMGFSFGATIVSNYLGEEGDK---CPIKTAVTISNPWDMIYSYEKMTF
313
BAO38417.1-Km
DP----NRKIYAVGFSFGATMLANYIGEEGDT---CLLSGATLLCNPWDLVLSANKMKT
284
XP_455042.1-Kl
DP----KRKIYAVGFSFGATMLANFLGEEGEK---CLLSGAALVCNPWDLTNSAHKMRE
285
CDH12365.1-Zb
HP----TRKIYAVGFSFGASLLANYLGEVGDK---SDLKAAVTLCNPWDFLLCSEKMKN
285
XP_002497841.1-Zr
NP----NRKIYAVGFSFGASLLANYLGETGSE---SNLTAAVTLCCPWDFLLCAEKMKK
286
AJW08323.1-Sc
FP----QRKIYAVGTSFGAAMLTNYLGEEGDN---CPLKAAVALSNPWDFVHTWDKLAH
291
EHM99903.1-Sc
FP----QRKLYTVGTSFGAAMLTNYLGEEGDN---CPLNAAVALSNPWDFVHTWDKLAH
291
EJT44508.1-Sk
HP----NRKLYAVGCSFGATMLGNYLGEEGDK---SPLSAAVTLCNPWDLLLSAVRMNE
287
XP_002554056.1-Lt
HP----SRKIYAVGFSFGATMLGHYLGEEGEK---APIEAASFLCNPWDLYQSALKMNQ
278
KGK36953.1-Pk
YELDDEKRQFHAIGYSFGGLQIANYLIKEGKN---TLLTSAITVSSPWNLYNSMLHISN
294
XP_002490078.1-Kp
IP----NKPIYLLGFSLGSALVLNYLGEEGKK---SFIKSAVTIGAPVDLLDSHYHLNH
281
XP_002550505.1-Ct
WP---KRPIYLVGFSFGAALTSNFLAEEGEKENPTNLVKAACTICCPWDFVDGAHHLQE 315
XP_001385510.2-St
YP---SRPIYAVGFSFGACILANFLGDPNHSEEDHNLIRAAALIGCPWDLVDSCYHLQQ 277
: :: :* *:*.
::: . .
::
:
KKA03693.1-Hu
DFWAKRLCSKNIVKFLVRTVEVNLPSLEKFREE----NVAKHTSNAHVFTKENMKKA-K 367
BAO38417.1-Km
DFWARKLFAKNITHFLVRTLEVNMNQVEWKGGE-KPTNVSPENPTNYPFTRENLKKA-K 341
XP_455042.1-Kl
DFWSSRLFAKSITQFLVRTLEVNMNQIEWKGGE-KPAEISPEHYSNYSFTRENLKKA-K 342
CDH12365.1-Zb
DYWSKNLFAKAITQFLTRLVKVNMGELESPDGSVPDHQPSIEDPCLYMCTPSNLERA-K
343
XP_002497841.1-Zr
DYWSKNLFSKAITQFLVRLVKVNMGELESPEGSKPEFQPDIENPCLYMCTKSNLERA-K
344
AJW08323.1-Sc
DWWSNHIFSRTLTQFLTRTVKVNMNELQVPENFEVSHKPTVEKPVFYTYTRENLEKA-E
349
EHM99903.1-Sc
DWWSNHIFSRTLTQFLTRTVKVNMKELQVPNDFKVSHKPTLEKPVFYTYTQENLEKA-E
349
EJT44508.1-Sk
DWWSKTLFSKNIAQFLTRTVQVNMGELGVPNGSRPDHPPTVKNPSYYMFTPDNLVKA-K
345
:: .
* *::
*
* ::
:
XP_002554056.1-Lt
DWWSRNLFSKNIAQFLIRLVKVNIKELEFKEGDVMPAEPASLEHPSFCVFTSKNLRKA-R
337
KGK36953.1-Pk
SWSGRYLFEPAVNSFLLRMVKNNKEVLKEN--P--------------IFSQERYDHLRK
337
XP_002490078.1-Kp
SYSGKYLFDPAVASFLSNLVKVNFPILNRDKPE--------------VFSYEKKSKD-Y
325
XP_002550505.1-Ct
SWTGKYLLSPALAKFLTKLVTNNYKELNKYIPD--------------IINENTLKTI-E
359
XP_001385510.2-St
SYSGNYLFNPALTTYLQKLVKNNYKTLSSFSPD--------------VFTDDLYREF-M
321
.: . :
: :* . : *
:
KKA03693.1-Hu
AFQTTAEFDDTFTAPYMGFNSAVEYYSNAGSINRLPNIKIPLLAINSEDDPVVGETSKSL
BAO38417.1-Km
QFTEPSQFDETFTSKAVGFDSAWDYYKVGSSLNRLPSINVPTLVINSHDDPVIGEENIPV
XP_455042.1-Kl
QFTDPSQFDDIFTAKAVGFEDSWSYYRVGSSINRLPTINVPTLVINSKDDPVIGDKCIPV
CDH12365.1-Zb
TFTQMRQFDDMFTAPSLGFSSSEDYYRAGSSINRLHNVQVPLLIINSKDDPIVGPQAIPY
XP_002497841.1-Zr
SFTQMLEFDGTFTAPSMGFSSAEEYYRAGSAINNLHKVQVPTLIINSTDDPIIDASSIPY
AJW08323.1-Sc
KFTDILEFDNLFTAPSMGLPDGLTYYRKASSINRLPNIKIPTLIINATDDPVTGENVIPY
EHM99903.1-Sc
KFTDILEFDSLFTAPSMGMPDGLTYYRKASSINRLPSIEIPTLIINATDDPVTGENVIPY
EJT44508.1-Sk
RFKSSLEFDEVFTAPALGFPNAMEYYNAASSINRVDTIRVPTLVINSRDDPVVGPD-QPY
XP_002554056.1-Lt
EFGSTAEFDNLFTAPCLGFDNAMDYYKACGSIHQLPNIKVPSLIINSKDDPVVGEDSIPY
KGK36953.1-Pk
TMKNSRDFDDAYTSKLLDLPSGDYYYYAASPVFQIYKIKTPLLVINSIDDPMISPD-YPF
XP_002490078.1-Kp
LVRRIVDFDRKYTAPAYGFASPKYYYQAGSPLTKLVNIYTPTVLLNSLDDPVVSCN-LPV
XP_002550505.1-Ct
NSKSLWEFDDNVTCKTTPYNSAFEYYKELSPVNKIDHIKTPLLALNSTDDPAVGVK-LPI
XP_001385510.2-St
KPKTTVEFDNVYTCHSAGFSNAMDYYREASPVRRIGNIKTPTLILNSTDDPTISVR-LPK
:**
*.
.
**
. : .: :
.
KKA03693.1-Hu
------- 475
BAO38417.1-Km
------- 448
XP_455042.1-Kl
------- 449
CDH12365.1-Zb
------- 450
XP_002497841.1-Zr
------- 451
. .
427
401
402
403
404
409
409
404
397
396
384
418
380
* : :*: ***
QNVILNNENVMYISTSLGGHLAYLTSDNDSFITEPVAQYLSSFEKHLA----EQA-KANPNILLVESDLGGHLAYLDRNYNPWITKQIAMYFDTLESFVK----KEA-EENPHVLLIETDLGGHLAYLEKNHDSWATKQVAQYFDALESLVK----TEA-KNNSNLLLLSTDLGGHLAFLNENFDSWINIQIASFLYKFDEFIE----SQV-KMNPNLLLLATDLGGHLAYLDETWDSWMNTHIASFFSTFDEFLV-----
AJW08323.1-Sc
KQA-RENPCVLLCETDLGGHLAYLDNENNSWLTKQAAEFLGSFDELVL----------- 456
EHM99903.1-Sc
KQA-KENPSVLLCETDLGGHLAYLDNENNSWLTKQAAEFLSTFDELVL----------- 456
EJT44508.1-Sk
SIV-EKNPRILYCRTDLGGHLAYLDNDNNSWATKAIAEFLTKFDELVV----------- 451
XP_002554056.1-Lt
KCA-KESDNLVLCVSDLGGHLAFLDKKYNSWATSKIAAFFDKFEELVQ----------- 444
KGK36953.1-Pk
LDISRHPWIYMATADLGGHYSFIKPNGDFWFSEVVEKWINSWREVDVQTPSDVLDHGWH
455
XP_002490078.1-Kp
DEVKANPYLYLAASDLGGHLAYIQWDGGFWFSEAVSAYFMAFESELSNEPAQSD----438
XP_002550505.1-Ct
SQM-KSNPYFCLVETDLGGHLGYIQQNGEFWCVELAEQFFQKFEELIE----------- 465
XP_001385510.2-St
LEV-LENPYLAMIETDLGGHLSYAKRSGDFWSVELVEEFFTKFDKVVA----------- 427
.
:.**** .:
:
::
.
KKA03693.1-Hu
BAO38417.1-Km
XP_455042.1-Kl
CDH12365.1-Zb
XP_002497841.1-Zr
AJW08323.1-Sc
EHM99903.1-Sc
EJT44508.1-Sk
XP_002554056.1-Lt
KGK36953.1-Pk
XP_002490078.1-Kp
XP_002550505.1-Ct
XP_001385510.2-St
---------------- 475
---------------- 448
---------------- 449
---------------- 450
---------------- 451
---------------- 456
---------------- 456
---------------- 451
---------------- 444
VDVELPLRE------- 464
--YEPQLSEYSYKTGI 452
---------------- 465
---------------- 427
ACTIVIDAD 4: Con las mismas secuencias utilizadas anteriormente, lleva a cabo un
alineamiento múltiple en el programa BioEdit. Coloca las capturas de pantalla que muestren
todo tu alineamiento en esta herramienta a continuación: (20 pts)
OJO: Guarda tu alineamiento y adjúntalo junto a este archivo como parte de tu examen.
El color que esta en negro es la ultima columna donde se quedo capturado el alineamiento de
la captura anterior
ACTIVIDAD 5: Con las mismas secuencias utilizadas anteriormente, lleva a cabo un
alineamiento múltiple en el programa MEGA. Coloca las capturas de pantalla que muestren
todo tu alineamiento en esta herramienta a continuación: (20 pts)
La columna que esta subrayada es la ulrima columna que fue capturada en la imagen anterior
OJO: Guarda tu alineamiento y adjúntalo junto a este archivo como parte de tu examen.
José Eduardo
NP_009362.1
XP_449865.1
XP_003683476.1
XP_002552789.1
XP_456122.1
BAO41510.1
CDO96415.1
XP_458945.1
XP_001384628.1
Nadia Monserrat
NP_009681.3
XP_003678108.1
XP_003682044.1
XP_002496373.1
XP_003957038.1
XP_448139.1
XP_002554702.1
BAO42626.1
XP_451923.1
CDO92444.1
Elina
NP_011699.1
XP_003673626.1
BAO39503.1
XP_447204.1
XP_003956960.1
XP_002551949.1
XP_455767.2
XP_003681894.1
XP_002495415.1
CDO96109.1
Monserrat
NP_116614.1
XP_002497318.1
XP_002555845.1
XP_453299.1
CDO92918.1
XP_458703.2
BAO41899.1
KGK40407.1
XP_002549329.1
XP_001386952.1
Giovanna Paola
NP_013287.1
KOG97884.1
EJT43963.1
XP_003957186.1
XP_452281.1
CDO92741.1
XP_002554295.1
XP_003680738.1
BAO37707.1
XP_002497946.1
KGK35261.1
XP_459916.1
KGR22770.1
KKA02424.1
XP_503418.1
Rigoberto
EJT44508
AJW08323
XP002554056
EHM99903
CDH12365
XP002497841
BAO38417
XP455042
KKA03693
XP002550505
XP001385510
XP002490078
KGK36953
Mónica Maribel
EJT43925
XP002552712
NP011693
AAP72993
EHN02269
XP003685841
XP003673631
XP003956955
BAO39498
XP455762
CDO96103
CCK11680
Laura Nayeli
NP_013937.1
EJT44981.1
XP_003679211.1
XP_003955751.1
XP_003686337.1
XP_446136.1
CDO92888.1
XP_453331.1
XP_002553204.1
BAO41868.1
Abigail
NP_014769.3
EJT41333.1
XP_003681047.1
XP_002554867.1
XP_003957292.1
CDF91713.1
BAO39062.1
CDO94208.1
XP_002498079.1
XP_454060.1
Karla Daniela
NP_014555.1
NP_014032.1
XP_002555715.1
CDF87621.1
BAO40244.1
XP_456023.1
XP_003958311.1
CDO96207.1
CAA45739.1
Erick Emmanuel
NP_009362.1
XP_449865.1
XP_003683476.1
XP_002552789.1
XP_456122.1
BAO41510.1
CDO96415.1
XP_458945.1
XP_001384628.1
Paola Estefania
NP_009681.3
XP_003678108.1
XP_003682044.1
XP_002496373.1
XP_003957038.1
XP_448139.1
XP_002554702.1
BAO42626.1
XP_451923.1
CDO92444.1
Jimena
NP_011699.1
XP_003673626.1
BAO39503.1
XP_447204.1
XP_003956960.1
XP_002551949.1
XP_455767.2
XP_003681894.1
XP_002495415.1
CDO96109.1
Gissela Monserrat
NP_116614.1
XP_002497318.1
XP_002555845.1
XP_453299.1
CDO92918.1
XP_458703.2
BAO41899.1
KGK40407.1
XP_002549329.1
XP_001386952.1
Mariana Lizbeth
NP_013287.1
KOG97884.1
EJT43963.1
XP_003957186.1
XP_452281.1
CDO92741.1
XP_002554295.1
XP_003680738.1
BAO37707.1
XP_002497946.1
KGK35261.1
XP_459916.1
KGR22770.1
KKA02424.1
XP_503418.1
Teresita de Jesús
EJT44508
AJW08323
XP002554056
EHM99903
CDH12365
XP002497841
BAO38417
XP455042
KKA03693
XP002550505
XP001385510
XP002490078
KGK36953
Arám
EJT43925
XP002552712
NP011693
AAP72993
EHN02269
XP003685841
XP003673631
XP003956955
BAO39498
XP455762
CDO96103
CCK11680
Fátima Sarahí
NP_013937.1
EJT44981.1
XP_003679211.1
XP_003955751.1
XP_003686337.1
XP_446136.1
CDO92888.1
XP_453331.1
XP_002553204.1
BAO41868.1
Pedro de Jesús
NP_014769.3
EJT41333.1
XP_003681047.1
XP_002554867.1
XP_003957292.1
CDF91713.1
BAO39062.1
CDO94208.1
XP_002498079.1
XP_454060.1
Cesar Eduardo
NP_014555.1
NP_014032.1
XP_002555715.1
CDF87621.1
BAO40244.1
XP_456023.1
XP_003958311.1
CDO96207.1
CAA45739.1
Melissa
NP_009362.1
XP_449865.1
XP_003683476.1
XP_002552789.1
XP_456122.1
BAO41510.1
CDO96415.1
XP_458945.1
XP_001384628.1
Ángel Yair
NP_009681.3
XP_003678108.1
XP_003682044.1
XP_002496373.1
XP_003957038.1
XP_448139.1
XP_002554702.1
BAO42626.1
XP_451923.1
CDO92444.1
Itzel del Carmen
NP_011699.1
XP_003673626.1
BAO39503.1
XP_447204.1
XP_003956960.1
XP_002551949.1
XP_455767.2
XP_003681894.1
XP_002495415.1
CDO96109.1
Paulina
NP_116614.1
XP_002497318.1
XP_002555845.1
XP_453299.1
CDO92918.1
XP_458703.2
BAO41899.1
KGK40407.1
XP_002549329.1
XP_001386952.1
José María
NP_013287.1
KOG97884.1
EJT43963.1
XP_003957186.1
XP_452281.1
CDO92741.1
XP_002554295.1
XP_003680738.1
BAO37707.1
XP_002497946.1
KGK35261.1
XP_459916.1
KGR22770.1
KKA02424.1
XP_503418.1
Sofia
EJT44508
AJW08323
XP002554056
EHM99903
CDH12365
XP002497841
BAO38417
XP455042
KKA03693
XP002550505
XP001385510
XP002490078
KGK36953
Braulio Alejandro
EJT43925
XP002552712
NP011693
AAP72993
EHN02269
XP003685841
XP003673631
XP003956955
BAO39498
XP455762
CDO96103
CCK11680
Yareli Jazmín
NP_013937.1
EJT44981.1
XP_003679211.1
XP_003955751.1
XP_003686337.1
XP_446136.1
CDO92888.1
XP_453331.1
XP_002553204.1
BAO41868.1
Vanessa Fabiola
NP_014769.3
EJT41333.1
XP_003681047.1
XP_002554867.1
XP_003957292.1
CDF91713.1
BAO39062.1
CDO94208.1
XP_002498079.1
XP_454060.1
María del Sagrario
NP_014555.1
NP_014032.1
XP_002555715.1
CDF87621.1
BAO40244.1
XP_456023.1
XP_003958311.1
CDO96207.1
CAA45739.1
María Guadalupe
NP_009362.1
XP_449865.1
XP_003683476.1
XP_002552789.1
XP_456122.1
BAO41510.1
CDO96415.1
XP_458945.1
XP_001384628.1
Isela
NP_009681.3
XP_003678108.1
XP_003682044.1
XP_002496373.1
XP_003957038.1
XP_448139.1
XP_002554702.1
BAO42626.1
XP_451923.1
CDO92444.1
EXTRA 1
NP_011699.1
XP_003673626.1
BAO39503.1
XP_447204.1
XP_003956960.1
XP_002551949.1
XP_455767.2
XP_003681894.1
XP_002495415.1
CDO96109.1