THE EFFECTS OF BLOOD FLOW RESTRICTION TRAINING ON BICEP MUSCLE HYPERTROPHY AMONG YOUNG MALE ADULTS A Thesis Presented to the Faculty of the School of Arts and Sciences NU Laguna In Partial Fulfillment of the Requirements for the Degree of Bachelor of Science in Exercise and Sports Science Major in Fitness and Sports Coaching Submitted by: Ushlee Enrique A. Basas Elaine Jairusse R. Capellan Yuen Magdalena William Verbruggen March 2025 ii APPROVAL SHEET This thesis titled THE EFFECTS OF BLOOD FLOW RESTRICTION TRAINING ON BICEP MUSCLE HYPERTROPHY AMONG YOUNG MALE ADULTS has been prepared and submitted by Elaine Jairusse R. Capellan, Ushlee Enrique A. Basas, and Yuen Magdalena William Verbruggen who are hereby recommended for Oral Examination in partial fulfillment of the requirements for the degree of Bachelor of Science in Exercise and Sports Science Major in Fitness and Sports Coaching. ERICK VOLTAIRE P. TABING, RND, MSApN Research Adviser Approved in partial fulfillment of the requirements for the degree of Bachelor of Science Exercise and Sports Science Major of Fitness and Sports Coaching by Oral Examination Committee. Mr. Milpert John B. Maroto, LPT, MPESS Panel Chair Mr. Edward Jan D. Lirio, LPT, MPES Panel Member Ms. Gretchie C. Gara, LPT, MSBio Panel Member Accepted in partial fulfillment of the requirements for the degree of BACHELOR OF SCIENCE IN EXERCISE AND SPORTS SCIENCE MAJOR IN FITNESS AND SPORTS COACHING. MR. NEILSON A. SILVA, LPT, MAEd (Date) Dean, School of Arts and Sciences iii ABSTRACT Blood Flow Restriction (BFR) training involves the application of a device that occludes venous, but not arterial, blood flow to muscles during exercise to promote muscular hypertrophy using low resistance loads. BFR training has recently drawn much attention and interest in the exercise rehabilitation and fitness field. Thirty subjects were assigned with experimental (performed bicep lift with BFR bands) and control group (underwent traditional resistance training). The intervention was a 12-week long training program that measured bicep circumference; with pre and post intervention measurements. Results showed that both groups experienced a statistically significant increase in bicep muscle size, however, the BFR group had a higher percentage increase. The results support the potential of BFR training as an effective alternative to traditional resistance training for hypertrophy, especially for populations that may not tolerate high training loads. This study adds to the emerging literature supporting BFR as a legitimate training modality for hypertrophy while also supporting the concept of volume management and overload regarding a properly programmed hypertrophy-based training regimen extending its curvature in a useful manner. Keywords: Blood Flow Restriction Training, Muscle Hypertrophy, Resistance Training, Bicep Growth, Strength Training, Low-Load Training, Exercise Science iv I, Elaine Capellan, would like to thank my family and friends who have always helped me in my life by teaching me things that are important to my growth; to my partner whom I love so much, and has been a huge help in making this study. To the entire group of respondents and Sir Erick Tabing, I would not be here without all of you. To that I give you my unfaltering loyalty until the end of time. Lastly, to everyone who has helped give me the strength to be brave and to confront my fears, thank you. I, Ushlee Enrique A. Basas, would like to thank my family for their love and support. To my friends who are there to help me overcome any challenges on the way. To my teammates who believe I can be a student and an athlete at the same time. To Sir Erick Tabing, who is always willing to help me and understand my circumstances as an athlete. Finally, I want to thank God for listening to my prayers, for forgiving me for all of my transgressions, and for being my source of faith and hope on this journey. I, Yuen Magdalena William Verbruggen, would like to thank my family and friends for always motivating me in everything that I do and pushing me to do my best. To my partner that I am very grateful for, thank you for always being there for me to show your unwavering love and support and help me in many ways that you can. To my groupmates who are always considerate and appreciative with all the efforts of each one of us, to Mr. Cadel Evance C. Hualda, for guiding me and helping me through the process of this study, to Sir Jeremy Randell Go, for providing my educational needs, and to Sir Erick Tabing, for always making time to help us and teach us with the best of his knowledge, thank you. Your time, teaching, and efforts are highly appreciated. Lastly, I want to thank God for being my support system the entire process and providing me strength and wisdom to fulfill this study. v ACKNOWLEDGEMENT We are grateful to everyone who participated in our study, "The Effects of Blood Flow Restriction Training on Bicep Muscle Hypertrophy among Young Male Adults. We are particularly appreciative to our research adviser, Mr. Erick Voltaire P. Tabing, RND, MSc, for his helpful advice and insightful feedback along the process. Your patience, input, and support are extremely helpful in completing this study. We also want to thank the students who took part in our study and served their time to attend our sessions and complete the workout. We also appreciate the panel members' thorough review and critical input, which helped significantly improve the final content. A special thanks to the faculty of the School of Arts and Sciences, especially those in the Exercise and Sports Science faculty at National University Laguna, for their assistance and providing of resources. Finally, we would like to express our gratitude to our family and friends for their continuous support and unconditional love. Your trust in us is what motivates us to complete this work. This work is the result of this individual's efforts, and we are grateful to everyone who contributed. vi TABLE OF CONTENTS Content Page Title Page i Approval Sheet ii Abstract iii Dedication iv Acknowledgement v Table of Contents vi List of Tables x List of Figures xii List of Acronyms xiii Chapter 1 - INTRODUCTION 1 Background of the Study 1 Statement of the Problem 2 Hypothesis 3 Theoretical Framework 3 Conceptual Framework 5 Scopes and Limitations 7 Significance of the Study 7 Definition of Terms 10 Chapter 2 - REVIEW OF RELATED LITERATURE AND STUDIES 11 Overview 11 Blood Flow Restriction Training 11 vii Muscular Hypertrophy 12 Benefits of Blood Flow Resistance Training 14 BFR Training: A Tool for Reducing Muscle Weakness and Loss 15 BFR training in clinical musculoskeletal rehabilitation 15 Bands, pressure, and Low- load BFR training 16 BFR- Training Adaptations in Strength-Trained Athletes and Team-Sport 17 Athletes Muscle Strength and Structural Adaptations 17 Impact of Workout Intensity on Muscle Hypertrophy 18 Optimal Training Volume for Bicep Hypertrophy 18 Strength Training Methods for Bicep Hypertrophy 19 Tempo and Muscle Hypertrophy 19 Synthesis 20 Chapter 3- METHODOLOGY 21 Research Design 21 Sampling Method 22 Context and Participants 22 Research Instruments 24 Data Gathering Procedures 24 Equipment 25 Program Development 26 Program Rationale 29 Objectives of the Program 30 viii Program Assessment 35 Program Application 35 BFR Application 36 Bicep Circumference 36 Data Analysis 37 Statistical Treatment of Data 37 Ethical Considerations 42 Chapter 4- RESULTS AND DISCUSSION 44 Workout Program 44 Changes in Bicep Hypertrophy Between BFR and Conventional Groups 54 Difference between the control and experimental group on bicep circumference 58 Adjusted Difference of Bicep Circumference based on Volume 60 Chapter5 SUMMARY, CONCLUSIONS, AND RECOMMENDATIONS 64 Summary 64 Conclusion 65 Recommendation 66 References 68 Appendices 74 APPENDIX A – High Performance Gym Request Letter 74 APPENDIX B – Informed Consent for Bicep Muscle Hypertrophy 78 Training Participants APPENDIX C – Eight-week Mesocycle Training Program 80 APPENDIX D- CERTIFICATE OF STATISTICIAN 84 ix APPENDIX E- CERTIFICATE OF GRAMMARIAN Curriculum Vitae 85 86 x LIST OF TABLES Table No. Table Title Page 1 Average Age of Male Participants in BFR and Conventional Training Groups 23 2 Activation routine 27 3 Warm up Routine 28 4 Cooldown 28 5 Week 1 to 3 of the adapted twelve-week mesocycle training program 31 6 Week 4 to 6 of the adapted twelve-week mesocycle 32 training program 7 Week 7 to 9 of the adapted twelve-week mesocycle 33 training program 8 Week 10 to 12 of the adapted twelve-week mesocycle 34 training program 9 Demographic Profile of Participants 44 10 Adapted twelve-week mesocycle training program 51 week 1 to 3 11 Adapted twelve-week mesocycle training program week 4 to 6 52 xi 12 Adapted twelve-week mesocycle training program 53 week 7 to 9 13 Adapted twelve-week mesocycle training program 54 week 10 to 12 14 Comparison of Left Bicep Circumference Changes Between BFR and Conventional Groups Comparison of Right Bicep Circumference Changes Between BFR and Conventional Groups 55 16 Comparison of Bicep Circumference Changes Between BFR and Conventional Training Groups 59 17 Comparison of Post-Test Bicep Circumference and Training Volume Between BFR and Control Groups 61 15 56 xii LIST OF FIGURES Figure No. Figure Title Page 1 SAID principle by John and Sandage (2019) 4 2 Conceptual Framework 6 3 Comparison of Bicep Circumference Changes Over Time Between 58 BFR and Control Groups xiii LIST OF ACRONYMS BFR - Blood-Flow Restriction RT - Resistance Training RIR- Reps in Reserve RPE- Rate of Perceived Exertion 1 CHAPTER 1 INTRODUCTION Background of the Study Resistance training is a proven approach to stimulate muscular hypertrophy, strength and endurance. Conventional resistance training (RT) usually consists of lifting progressively heavier loads to elicit muscle adaptation. But for those recently introduced to resistance training, people with injuries, or those with joint restrictions, this highintensity approach can be impractical. This has propelled researchers and fitness professionals alike to investigate alternative methods, including Blood Flow Restriction (BFR) training. BFR training is the use of specialized bands or cuffs that partially occludes venous return but still allows arterial flow. This technique immerses the muscle in a state of hypoxia, which elevates metabolic stress and improves fast-twitch muscle fiber recruitment which is an important stimulus for hypertrophy (Sieljacks et al., 2019). While BFR training can be performed at very low intensities (20-50% one-repetition maximum) and still produce muscle growth comparable to traditional high-load resistance exercise (Chang et al., 2024). This is part of the reason why BFR has become a popular strategy in rehab settings and is also used by athletes hoping to achieve hypertrophy without damaging their joints with excessive mechanical tension. The gap in the current body of evidence to support the usage of BFR training over traditional exercise has become apparent, and in part, this research study aims to fill that gap. Many studies have been conducted on clinical populations, for example post-surgery patients or older adults, meaning they have limited applicability to active people. Additionally, the most effective training parameters for BFR 2 have not been established and therefore require investigation within rigid hypertrophy programs (Hernández-Martín et al., 2024). This study aims to fill these gaps by comparing effects of 3 weeks of BFR training on hypertrophy of the bicep muscle of young male adults (18–30 years). The study seeks to directly compare BFR to traditional training and provide evidence-based information that exercise professionals, athletes, and rehabilitation experts can use to leverage hypertrophic mechanisms in training to educate fitness trainers and rehabilitation professionals on the possible use of BFR in strength and conditioning programmers. However, while more traditional hypertrophy resistance training is still considered the gold standard for muscle hypertrophy, BFR alternative training may provide at least comparable results more work is needed to validate these assertions empirically. The prospective hypertrophic potential of BFR training will be evaluated relative to traditional training, and the feasibility of using BFR to promote accelerated bicep muscle growth with minimal mechanical tension will also be explored. Statement of the Problem This study intends to find out how blood flow restriction training can affect bicep hypertrophy. The researchers ought to find out the current preferred style of training in the community. Also, it serves as a guide to the newly interested individuals in order to be more knowledgeable in this area thus significantly reducing the possibility of underlying accidents. This study aimed to determine the effects of Blood Flow Restriction training on bicep muscle hypertrophy among young adults. This study will be conducted during S.Y. 2024-2025 at National University, Laguna. 3 Specifically, this study sought to answer the following questions: 1. What is the demographic profile of the respondents in terms of: 1.1. Sex 1.2. Age 2. What type of program will be applied for the bicep hypertrophy? 3. What is the change of the bicep circumference of the participants who undergo BFR training before and after the duration of the study? 4. What is the change of the bicep circumference of the participants who did not undergo BFR training before and after the duration of the study? 5. Is there any significant difference between the control and experimental group on bicep circumference? Hypothesis H0 - Bicep muscular hypertrophy will have no significant difference before and after using blood flow restriction (BFR) training. H1 - Bicep muscular hypertrophy will have significant difference before and after using blood flow restriction (BFR) training THEORETICAL FRAMEWORK This study is grounded in the Specific Adaptation to Impose Demand (SAID) principle as articulated by Johnson and Sandage (2019). The SAID principle suggests that the body adapts specifically to the demands placed upon it. When subjected to a particular type of stress or exercise, the physiological adaptations that occur in the body 4 are specific to that type of stress. This principle is crucial for understanding how different training methods can lead to varying adaptations in muscle growth, strength, and endurance. With enough use, volume can now be equated to fit the program made for the participants based on their volume landmarks. With the proper usage of these variables, BFR training can now be given. The idea is that your body responds to the exercise you perform. In particular, your body adjusts to the particular exercise you perform; for example, lifting weights increases muscular size. After that, your body will adjust to the exercise you do. Changes can include improvements in muscle growth, speed, endurance, and other areas. To put the SAID principle into much simpler terms, our study indicates that if an individual wants to increase strength and muscle size, they should lift weights. An individual's actions impact the demands they place on their body. Figure 1. SAID principle by Johnson and Sandage (2019) There are three (3) adaptations that can happen to the participant, and they are listed inside the framework. Neurological adaptations happen when loading increases overtime, making the athlete stronger and better using motor units as their bodies become more efficient. Morphological adaptations are those changes in the physical form of an 5 athlete's body. This can be seen through changes in the body composition by increased muscle mass or a decrease in body fat. Lastly, bioenergetic changes refer to the internal mechanisms of the body. This can be an increase in stroke volume as their bodies become more efficient in using their blood and better metabolism due to higher activity; it can also be the increase in BMR due to the increased muscle mass they have gained from their training. If BFR is used in the study for adaptation, the participants' bodies should undergo significant adaptations. According to Jørgensen et. al (2023) BFR training enhances muscle protein synthesis, increases muscle fiber activation, and induces metabolic stress, which collectively contribute to muscle growth and strength gains even at lower training loads. This makes BFR-RT a valuable method for individuals who may not be able to perform high-load exercises due to various clinical musculoskeletal conditions The outcomes are now the effect of the training variables that have been acted upon the athlete’s body. The framework gives us a good view of how adaptations happen overtime, and their possible effects on performance. In the context of this study, the participants should gain adaptations through hypertrophy training, which shall be programs for them to increase the needed variables. CONCEPTUAL FRAMEWORK This conceptual framework illustrates the steps involved in researching the Effect of Blood Flow Restriction Training on Bicep Muscle Hypertrophy. It begins with an exercise program, namely the bicep hypertrophy workout plan, which will be implemented among the participants. There will be pre- and post-test measurements of the participants' 6 mid upper arm circumference before and after the workout program is implemented to establish a baseline and compare the results at the end of the process. Figure 2. Conceptual Framework There will be sets and repetitions throughout the workout, but Machine Bicep Curl consists of more sets than free weights because the machine requires less stability from the body, resulting in higher muscle bias in the biceps. Because of the increased instability of the movement, free-weight workouts need stronger muscle coordination to be performed correctly (Haugen et al. 2023). A mid-upper arm circumference assessment will be performed to get an initial measurement of the bicep muscle, which will be used to compare with post-test results. After the pre-test, the participants will be divided into two groups: experimental and control. The experimental group will use BFR bands during the program, while the control group will undergo conventional training. Next, the workout program will be implemented. The purpose is to see if BFR training could stimulate muscle growth compared to standard training. After completing the training program, a post-test to assess the changes in bicep muscle growth will be conducted. 7 Scopes and Limitations of the Study The study will be conducted on 22 young male adults in NU Laguna to assess the effects of blood flow restriction training on bicep muscle hypertrophy. Participants will be evaluated based on their training history, gender, weight, and body fat percentage. For three months, ten individuals will perform BFR training while the remaining ten will do conventional training without BFR bands. The following equipment will be used in the study: measuring tape, BFR bands, skinfold caliper, and sphygmomanometer. The study only looked at biceps hypertrophy, not other muscle groups. The results were limited to the parameters measured, which were the mid upper arm circumference, and the study sample consists of young adults from NU Laguna. Significance of the Study This study intends to find out how blood flow restriction training can affect the bicep hypertrophy. The researchers ought to find out the current preferred style of training in the community. Also, it serves as a guide to the newly interested individuals in order to be more knowledgeable in this area thus significantly reducing the possibility of underlying accidents. Specifically, this study could be beneficial to the following: Health and Fitness Promotion This research will advance knowledge of efficient training techniques and raise awareness of the importance of fitness and health. This study will offer important insights that will assist individuals and fitness professionals in optimizing exercise 8 routines for better health and aesthetic outcomes by contrasting blood flow restriction (BFR) training with conventional approaches. Boosting the Fitness Industry in the country This study will aid in the development of local fitness programs, as fitness is gradually gaining popularity in this country. The results of the study can assist organizations and fitness professionals in creating inventive and more successful training regimens. This could stimulate the fitness sector, resulting in job creation and economic expansion. Contribution to Existing Body of Knowledge in the Field of Sports and Exercise Science This will advance the study of sports and exercise science by expanding the understanding of training techniques and optimizing training protocols. This will also contribute to the field of sports and exercise science. Through comparison, the research will add depth to the understanding of how different training methods impact muscle hypertrophy. This can refine existing knowledge about the relative effectiveness of various training techniques. Furthermore, the mechanisms of hypertrophy in the study can elucidate the specific physiological mechanisms underlying muscle growth with BFR training. Understanding these mechanisms in contrast to conventional methods advances the scientific knowledge of how different stimuli affect muscle adaptation. Additionally, determining the ideal BFR training parameters—such as cuff pressure, duration, and frequency—contributes important data for improving training regimens. This will enable academics and practitioners in the area to make more precise suggestions. 9 The conclusions of the study can help determine the optimum ways to apply BFR training in a variety of contexts, such as general fitness, sports training, and rehabilitation. This helps create training programs that are more evidence-based and effective. Promotion of Value and Social Relevance Blood flow restriction training is a training technique that promotes muscle growth even while using relatively light weights. This study aims to explore the effectiveness of BFR training in growing muscle size in young adults. By proving the accessibility, affordability, and benefits of BFR training, this study could encourage healthier lifestyles, promote training methods, and help to the development of new rehabilitation training approaches. 10 DEFINITION OF TERMS Hypertrophy is the process of developing and increasing muscle cells. "Hypertrophy" refers to the enlargement of muscles caused by activity. Lifting weights is the most common way to increase hypertrophy during exercise if you want to tone or improve muscle definition. Blood Flow Restriction Training is a new method that uses a cuff/tourniquet system to maintain arterial flow while restricting venous return. According to Wortman (2020), BFR training involves putting a cuff or tourniquet over the proximal end of an extremity and inflating it to a predetermined pressure (ranging from 110 to 240 mm Hg) to maintain arterial flow and restrict venous return. Conventional Training is a weightlifting technique that focuses on increasing muscle size and definition. It is usually called traditional bodybuilding that typically involves using gradual resistance exercises to overload muscles and induce growth. Mid upper arm circumference or MUAC, is the measurement of the upper arm. It is located at the midpoint between the top of the shoulder and the tip of the elbow. 11 CHAPTER 2 REVIEW OF RELATED LITERATURE This chapter presents concepts and theories about the effects of blood flow restriction training on bicep muscle hypertrophy. The content specified herein will tackle literature from reliable authors that will supply information and ideas about the use and effects of blood flow restriction training on bicep muscle hypertrophy. Blood Flow Restriction Training Blood flow restriction (BFR) training is a method that mimics the effects of high intensity training by combining low intensity exercise with blood flow obstruction. Although it has been around for a while in the gym, its application in therapeutic settings is growing. There are two types of BFR bands mainly, pneumatic and non-pneumatic. A "pneumatic BFR band" uses air pressure within an inflatable cuff to restrict blood flow through the limb, reducing the flow without completely cutting it off. Separately, a "nonpneumatic BFR band" applies pressure through a mechanical means rather than relying on an air-filled bladder; these bands utilize straps, buckles, or other tightening mechanisms to gradually reduce blood flow below the limb. Both styles of bands emulate the effects of exercise by impeding circulation to induce muscle fatigue and stimulate muscle growth and recovery through restricted oxygen and nutrient delivery, even with reduced exertion levels. (Krzysztofik et al., 2019) According to Wilson (2021) the practice of blood flow restriction (BFR) training is becoming more and more common in weight rooms. That does not imply that it is fully understood, though. Indeed, the confusion seems to be increasing with all the different titles 12 (occlusion training, hypoxic training, KAATSU), styles (bands, cuffs, ace bandages), and objectives attached to this kind of training. The initial process of muscle growth is made possible by this arrangement, which causes the muscle to enlarge. An accumulation of metabolites like lactic acid, which have been demonstrated to directly drive muscle growth, is another effect of blood flow restriction. Furthermore, the neurological system is compelled to engage the largest fast-twitch muscle fibers, which have the highest potential for growth, because to the direct fatigue that is imposed on the muscles. The BFR training includes various parameters that have a major impact on training adaptations and must be carefully considered. These factors include resistance load, volume, cuff pressure, cuff width, duration of BFR, and BFR type (continuous or intermittent). Among these characteristics, cuff pressure is regarded as a significant determinant for attaining effective training adaptations while maintaining safety. Studies have found that acute physiological responses and long-term physiological adaptations are pressure dependent. Applying the same absolute pressure to different individuals may lead to inconsistent BFR levels. Ensuring that all participants in a group receive a similar BFR stimulus is essential for consistency in research (Wang et al., 2022b). In clinical practice, it is crucial to avoid excessively high pressures due to potential safety concerns (Patterson et al., 2019). Muscular Hypertrophy Muscle cell development and increase is known as hypertrophy. The term "hypertrophy" describes the enlargement of muscles brought on by exercise. Lifting weights is the most popular method of increasing hypertrophy during exercise if your goal is to tone or improve muscular definition. The term "muscular hypertrophy" describes the 13 growth of muscle cells (Hough et al., 2019). You can lift a heavy weight for fewer repetitions (reps) when weightlifting, or you can lift a low weight for numerous reps. How you lift will dictate how your muscles develop and evolve. For instance, using a lesser weight can help you build muscle tone, but increasing the number of repetitions needed to increase muscle fiber efficiency will be necessary. You won't see much muscle definition with this type of training unless you exhaust yourself doing a lot of repetitions. However, lifting large weights is a good method of encouraging muscle fiber growth and definition. If time is of the essence, this method of exercising is also more effective. When muscle protein synthesis surpasses muscle protein breakdown and produces a positive net protein balance over time, muscular hypertrophy is the outcome. Possible when using both RT and protein consumption, it increases the synthesis of muscle proteins and reduces their breakdown. From a nutritional perspective, protein consumption combined with RT is a powerful inducer of muscle protein synthesis. To optimize muscle adaptations, RT's variables such as effort level and intensity, exercise sequence, repetitions and sets completed, movement speed, length of rest intervals between sets and exercises, and training status have been thoroughly studied and debated. An effort's volume and intensity are fundamental elements that directly affect how muscles adapt (Krzysztofik et al., 2019). Numerous studies have shown that when fatigue sets in, training at low loads produces hypertrophy that is comparable to training at moderate and high loads. Additionally, it is not always required to approach fatigue to achieve notable hypertrophy gains—especially when high-load training is considered. Research suggests that the majority of training sets with ~3–4 repetitions in reserve (with moderate to high-loads) result in considerable muscle hypertrophy. Additionally, it has been demonstrated that the 14 volume of resistance training (RT), which is the total number of repetitions plus the loads applied for a particular exercise, is the primary factor in adaptation for muscle hypertrophy. Additionally, it is established that maximizing muscle hypertrophy requires the manipulation of training volume, intensity of effort, and range of motion (Androulakis et al. 2023). Benefits of Blood Flow Resistance Training An elastic band, or tourniquet, is wrapped around your arm or thigh to stop blood flow and deceive your body into believing that you are exerting more effort than you are. Adding BFR training to your fitness regimen can be quite beneficial, particularly if you're having an undergoing surgery or have osteoporosis and are unable to lift enough weight to encourage muscle growth (Nascimento, 2022). The notion that "no pain, no gain" which is applicable to muscular hypertrophy, is not too dissimilar from what science has to say about the process. Strength training causes an alteration in the internal milieu of the muscles, which triggers hypertrophy. Blood still enters your muscles through your arteries even when you exercise for BFR. However, wearing the band prevents it from vanishing through your veins. This disruption of regular blood flow makes it possible for your muscles to get fatigued, swollen, and oxygen-depleted faster. Applying an external pressure, usually with a tourniquet cuff, on the closest part of the upper and/or lower limbs is the technique of BFR in the muscle utilizing a pneumatic tourniquet device. When the cuff is inflated, the vasculature beneath it gradually compresses mechanically. This partially restricts arterial blood flow to structures farther away from the cuff, but it has a greater negative impact on venous outflow from beneath 15 it; which is thought to also obstruct venous return. A lack of oxygen (hypoxia) in the muscle tissue is caused by compression of the capillaries near the skeletal muscle (Patterson et al., 2019). Moreover, blood pools in the occluded limbs' capillaries due to the reduction in venous blood flow, which is frequently shown as erythema. The amount of pressure exerted may have an impact on the degree of blood pooling. Furthermore, there is an increase in intramuscular pressure behind the cuff during muscular contractions conducted in BFR circumstances, which further disrupts blood flow. BFR Training: A Tool for Reducing Muscle Weakness and Loss According to Lorenz et al. (2021) muscle weakness and loss are common outcomes of musculoskeletal injury. Blood flow restriction training has the potential to reduce weakness and muscle loss without overloading recovering tissues. It appears to be an effective and safe method for therapeutic exercise in sports medicine settings. This technique requires careful evaluation of variety of factors, thus the objective of this paper is to provide insights into proposed mechanisms of effectiveness, safety concerns, application guidelines and clinical recommendation for BFR training after musculoskeletal injury. Whereas high-load training produces the greatest increases in strength and muscle hypertrophy, BFR training appears to be the viable option for connecting earlier phases of rehabilitation when the patient may not tolerate higher loads and later stages that are consistent with a return to sport. BFR training in clinical musculoskeletal rehabilitation According to Jorgensen et al. (2023), a review with meta-analysis found that low load resistance training with BFR to the exercising limb and high load resistance training are equally effective for achieving improvements in skeletal muscle mass in the healthy 16 population. As a result, blood flow restriction training has been proposed as a potential exercise approach in clinical populations where either fragile post-surgical condition or injury may limit patients' ability to exercise at greater muscle loading intensities. Immobilization causes muscle mass and strength loss, which is a well-known problem in clinical populations. According to Balchandran et al. (2022), muscle mass and strength have been related to decreases in physical function, which may result in chronically lower physical function. Recovering strength and muscle mass after a prolonged period of bed rest is difficult, and as a result, muscle strength deficiencies persist despite post-injury therapy. As a result, it is considered extremely important for patients to participate in exercise-based activities that promote skeletal muscle mass and mechanical functions in order to counteract the negative impact of disease burden and disuse on muscle morphology, maximal muscle strength, and function performance (Currier et al. 2019). Bands, pressure, and Low- load BFR training In the study by Rockhill et al. (2020), the bands are pneumatic or operated by air under pressure, and when inflated, they use a "barrel" system that allows for more elasticity and non-uniform circumferential pressure, reducing the risk of arterial blockage such as ischemia-perfusion injury or rhabdomyolysis. The researchers also state that during BFR training, a hypoxic environment or low oxygen levels in the muscle cause the buildup of metabolites, which are waste products created by the muscles during exercise and could potentially stimulate muscle growth. Metabolites such as lactate, a hormone that aids muscle growth, stimulates enhanced growth hormone and rapid twitch fiber recruitment, allowing for hypertrophy and strength development even at lower weights. Low-load BFR 17 training can increase muscle growth and exercise tolerance while lowering the risk of injury associated with high-load resistance training. BFR- Training Adaptations in Strength-Trained Athletes and Team-Sport Athletes As stated by Pignanelli et al. (2021), most studies focused on the efficacy of BFR training in improving muscle strength and muscle growth among untrained individuals. Other studies shows that BFR resistance exercise has influence on the adaptations of trained individuals. Haugen et al. (2023) conducted a 6.5-week strength program. They added two 1-week blocks consisting of front squats at either 1) moderate loads (~30% 1repetition maximum) with continuous BFR or 2) conventional high loads (~60%-85% 1RM) for national-level power lifters. Regardless of advanced training condition, 10 sessions of BFR increase quadriceps muscle size by 3%-8%, muscle fiber by 12%, and the number of myonuclei by 18%, supporting the increased size of muscle fibers that can promote muscular growth and capillary-to-muscle fiber connections (12%) are found in type-I muscle fibers, which connect to muscle cells and improve blood flow, delivering oxygen and nutrients. There are no changes in these variables in the conventional training group. Muscle Strength and Structural Adaptations Most strength and conditioning programs aim to assist athletes enhance their force producing capability in order to increase power output. Indeed, enhancing an athlete's muscle strength can affect submaximal or moderate intensity exercise, as well as the capacity for explosive movements. A meta-analysis by Grønfeldt et al. (2020) found that low-load BFR-resistance training improves muscle strength as much as high-load resistance training in untrained and recreationally active individuals. 18 At the microscopic level, myofibrillar protein synthesis, which is a way to make our muscles bigger and stronger, increased by 10% after a single session of BFR-resistance exercise compared with a worked-matched control individual (Nyakayiru et al. 2019), and with training, myofibrillar protein synthesis increased comparably between low-load BFR and high-load resistance training in exercise naive individuals (Sieljacks et al. 2019).When low-load exercise is performed until volitional fatigue, with or without BFR, muscle size increases by 5% to 12% in both exercise naive and resistance trained individuals (Pignanelli et al. 2020). Currently, no research has explored how training state affects the hypertrophic response to BFR exercise. Nonetheless, based on current information, BFR training requires less effort at similar external intensities/loads to elicit a significant hypertrophic training response, which is critical for maximizing muscle mass development during certain training phases. Impact of Workout Intensity on Muscle Hypertrophy According to Kassiano et al. (2025), which studied the muscle development and strength adaptation by performing unilateral and bilateral resistance training, planned progression in weight training can result into a significant muscle hypertrophy. Currier et al. (2022) contrasted the idea and discovered that higher load and multiple set training were great for strength development and different training prescriptions improves muscle hypertrophy. This shows that various methods can be effective to induce muscle hypertrophy. Optimal Training Volume for Bicep Hypertrophy Hoseinpour et al. (2025) discovered that performing 20-30 sets per muscle group each week yielded the best results for muscle growth. Participants who followed this 19 regimen experienced significant increases in both muscle size and strength over six weeks. On the other hand, Robinson et al. (2024) found that training to failure was not necessary for muscle growth. Instead, they observed that moderate effort combined with sufficient volume can also lead to substantial hypertrophy. Strength Training Methods for Bicep Hypertrophy According to Currier et al. (2022) study, which aimed to determine how different combinations of resistance training factors like load, strength, and frequency can affect muscle strength and hypertrophy. Krzysztofik et al. (2019) did another study and found that low-load resistance training under BFR increased cross-sectional area, even before the lifters got tired of working out. Tempo and Muscle Hypertrophy According to Zajac et al. (2021), unintentionally slow tempo can occur during resistance training when heavy weight or tiredness shows up as a slower movement. An intended slow tempo can be employed when the load is light enough to regulate and fatigue does not interfere with an individual's control over the velocity of the action. Wilk et al. (2019) reported contrasting ideas, that faster tempos may be more important for neural development and strength movements, whilst slower tempos extend time under tension, causing hypertrophy. This means that there is a knowledge gap about the best tempo for maximal hypertrophy, and more research is needed to establish the effects of various tempos on muscle growth and strength development. 20 Synthesis To recapitulate, all these concepts are examined and support this study. The notions of low-load blood flow restriction training with bicep muscular hypertrophy and traditional training on bicep muscle hypertrophy have partially similar theories, and researchers must determine which is more effective for developing bicep muscle in young male adults. Additionally, how can BFR training be an effective approach to bicep muscle hypertrophy with its high cost and limited accessibility restricting its use in clinical and practical applications? To conclude, the concepts included are viable and can be used to increase bicep muscle circumference. The objective of the study is to understand the effects of BFR Training on bicep muscle hypertrophy and assess the average size difference after the experiment. 21 CHAPTER 3 METHODOLOGY This chapter includes information regarding the research design used for the study, the sample and sampling design, the context and participants that were included, the research instruments that were used, the data gathering procedure, data analysis, and ethical considerations. Research Design This study used a quantitative experimental method due to the amount of numerical data that was gathered from the bicep muscular circumference of the respondents from pretest and post-test using BFR training and conventional training between two groups. This study was also experimental in nature due to the processes and outcomes the respondents went through to achieve the results that were gathered from the intervention. The study also aimed to explore the diversity of muscular hypertrophy, specifically bicep muscular hypertrophy; hence, the use of standard measurements and equipment was utilized to acquire the best results relevant in the study. The researchers aimed to determine the potential impact of BFR training on bicep muscular hypertrophy in terms of the important parameters used in the training, which were cuff pressure, duration, frequency, and lastly, muscle growth. This method was intended to determine how BFR training was effective for bicep muscular hypertrophy using the parameters specified that served as a guide. 22 Sampling method The researchers used purposive sampling method to identify young male participants among the 22 individuals divided into two groups: 12 for the experimental group going through BFR training and 10 for the control group doing conventional training. The study included participants depending on their weight, ranging from 60 kg to 90 kg. According to a study by Helms et al. (2023), male trained individuals hovered their bodyweight as low as 77 kilograms to as high as 85.9 kilograms. This paper gave a hypothesis of what body weight range could be used for this study. To gain more muscular mass and strength, many people engaged in resistance training (RT). It was encouraged to have energy surpluses to sustain these improvements, but if they were excessively high, they may result in unneeded fat growth. Participants were separated into two groups depending on weight: control and experimental. Using the purposive sampling method, researchers selected individuals who they believed would provide the most important data for their research objectives. Context and Participants The study participants were individuals between the ages of 18 and 30 with no history of coagulation disorders, including deep vein thrombosis. Exclusion criteria for this study included pregnancy, smoking, lymphoedema, cardiovascular disease, chronic degenerative diseases, a history of cancer, recent surgery in the lower limbs within the past 12 months, or medication use affecting blood flow regulation. They primarily came from NU Laguna. Respondents also had less than a year of experience in weightlifting or bodybuilding. There was a total of 22 respondents, with 10 in the control group and 12 in 23 the experimental group. Respondents who had experience with resistance/strength exercises were thoroughly instructed about the study. Before the study began, students were given the physical activity readiness questionnaire and any other paperwork that had to be completed in order for the researchers to collect the necessary data from all participants. Studies using small sample sizes are common in BFR research due to the precision of measurements and focus on targeted interventions. For example, in trials examining BFR's effect on muscle strength and hypertrophy. This was especially true when the emphasis was on obtaining detailed physiological outcomes or when practical constraints such as time and resources limited larger cohorts. Such studies successfully showed significant hypertrophic responses with BFR, suggesting that a smaller but wellcontrolled participant pool could still yield meaningful and generalizable results when appropriately designed (Chen et al., 2024). Previous reviews and systematic analyses have found that even with limited sample sizes, BFR training can deliver outcomes comparable to high-load resistance training for hypertrophy. This suggests that smaller-scale studies remain a valid approach to exploring BFR's targeted effects on specific muscle groups (Hernández-Martín et al., 2024). Table 1. Average Age of Male Participants in BFR and Conventional Training Groups sex Average age BFR group Male= 12 19 ± 0.82 Conventional group Male=10 18.6 ± 0.92 24 Research Instruments The BFR bands and an approach for measuring bicep muscle served as the primary study instruments. The researchers used a quantitative experimental design to assess the effect of BFR training on bicep muscle hypertrophy. The procedure specified that the participants' biceps were measured at the midpoint of the upper arm with a measuring tape. Measurements were gathered before and after the BFR training. To assure the precision and reliability of the BFR bands that were used, we employed a 7 out of 10 perceived tightness rating. Centner et al. (2018) proposed this method of applying pressure based on the individual's impression of tightness. The authors discovered that no subjects were experiencing vascular blockage when the restriction was applied. Data Gathering Procedure The researchers submitted a preliminary letter to the Inspire Sports Academy Office requesting permission for the researchers and participants to have access to the Multipurpose Gym and gym equipment for the study. To select participants, the researchers published information about the study on social media to see if anyone was interested in participating. The researchers also asked participants around NU Laguna. After gathering participants, the researchers used a PARQ to determine whether the participants should consult with a medical professional, check their health status to ensure their safety, and to identify any potential health risks before performing the exercise. Then the researchers created a bicep training program for the participants. The researchers conducted a study using a 12-week bicep hypertrophy program. It consisted of various exercises for the bicep muscle. An expert in sports science validated the program's safety and effectiveness. They 25 also conducted a mid-upper arm assessment, in which the researchers measured the participant's mid upper arm circumference. Using the participants' biceps circumference as a baseline for this study. The data collected in the study was based on the participants' middle upper arm circumference. From there, the researchers compared the participant's bicep circumferences before and after the study to see the results. Following the pre-test, participants were separated into two groups: experimental and control. The experimental group used BFR bands throughout the program, while the control group received conventional bicep training. After completing the training program, the researchers conducted a post-test evaluation in which they measured each participant's biceps mid upper arm circumference to assess increases in bicep muscle growth. Equipment In accordance with the standard measurements and equipment to be used, the researchers used blood flow restriction bands purchased from a well-known fitness brand called BFR BANDS, which are known for their equipment that is highly durable and effective in restricting blood flow. The wearable BFR training device had good validity (ICC = 0.85, mean difference = 4.1 ± 13.8 mmHg [95% CI: −23.0 to 31.2]), excellent interrater reliability (ICC = 0.97, mean difference = −1.4 ± 6.7 mmHg [95% CI: −14.4 to 11.7]), and excellent test-retest reliability (ICC = 0.94, mean difference = 0.6 ± 8.6 mmHg [95% CI: −16.3 to 17.5]) for assessing AOP. These findings were consistent across male and female groupings (Zhang et al. 2024). 26 BFR tightness scale The researchers used a perceived tightness grading of 7 out of 10 to ensure the precision and reliability of the BFR bands they used. Various restrictive pressures had been used during blood flow restriction exercise, including those depending on brachial systolic blood pressure, limb size, and resting arterial occlusion pressure. Among these strategies, adjusting the pressure relative to the arterial occlusion pressure was preferred since it considered both the cuff and the limb size. This guaranteed that the pressure applied restricted but did not completely block artery inflow, a critical concern for participant safety. Although getting a percentage of resting arterial occlusion pressure was useful for standardizing between individuals, not all practitioners could access this equipment. Wilson et al. (2013) proposed a pressure application method for elastic knee wraps depending on the individual's perceived tightness. The authors found that restricting individuals to an experienced tightness of 7 out of 10 did not result in arterial blockage. Program Development The effects of blood flow restriction training on bicep muscle hypertrophy in young male adults were studied implementing a twelve-week bicep workout program. This study included two groups: a BFR group and a conventional group. The program was designed with increasing overload. Weeks 1-3 concentrated on foundational movements, including workouts such as one-arm bicep curls, barbell bicep curls, and closed grip barbell bicep curls performed with 8-12 repetitions for 2-3 sets with a 2–3-minute rest break. Weeks 4-6 of the program included workouts that focused on peak contraction and isolation. This phase consisted of 8-12 repetitions of concentration curls, cable bicep curls, 27 seated bicep curls, and barbell preacher curls, with sets raised to 3-4 sets and rest intervals extended to 3-4 minutes due to the increased intensity. In this six-week training program, blood flow restriction bands were used to apply pressure and enhance hypertrophic response. The conventional group performed the same workout routine, but without the use of BFR bands. Bicep circumference measurements were performed at the beginning, weekly, and end of the training program to evaluate and compare the effectiveness of the training methods. This program was developed to use blood flow restriction in traditional resistance training to enhance hypertrophic response and determine whether it was more efficient than the usual training method. This table shows the activation routine which begins with shoulder rotations 30 seconds forwards and backwards followed by resistance band curls for 2 sets of 15-20 reps and standing bicep stretch for 30-60 second hold. This is intended to activate the muscles for the activity. Table 2. Activation routine Exercises Shoulder rotations Resistance band curls Standing bicep stretch Time 30 seconds forward, 30 seconds backward 2 sets of 15-20 reps 30-60 second hold This table shows the warm-up routine, which begins with a 5-minute treadmill run and is followed by three dynamic stretches of 30 seconds each, performed in each direction. This is intended to increase the heart rate and prepare the muscles for the activity. 28 Table 3. Warm up Routine EXERCISES Treadmill run Arm circle R/L Cross-body shoulder stretch Arm swing front to back TIME 5 min 30 sec 30 sec 30 sec This table shows the cooldown routine, which begins with a 5-minute treadmill exercise at a slow pace to bring the heart rate back down. Followed by three static stretches, each lasting 30 seconds to enhance flexibility and reduce muscle pain. This is intended to lower the heart rate and help in recovery from the workout. Table 4. Cooldown EXERCISES Treadmill Static Stretching Bicep stretch L/R Shoulder stretch TIME 5 min 5 min 30 sec 30 sec Table 5 shows weeks 1-3 of the bicep-focused workout program. It includes four exercises which are the one arm bicep curl, barbell curl, preacher curl and closed grip barbell curl. Load is set at 40% of with 15-20 repetitions and 2-3 sets and an RPE of 3-7. Tempo is 3:0:1 and the rest interval is 2-3 minutes. The rationale for each exercise is to build bicep strength and increase size while maintaining proper form and minimizing injury risk. Table 6 shows weeks 4-6 of the bicep-focused workout program. It includes four exercises which are the concentration curl, cable bicep curl, seated bicep curl, and barbell 29 preacher curl. Load is set at 60% of with 12-15 repetitions and 3-4 sets and an RPE of 4-8. Tempo is 3:0:1 and the rest interval is 2-3 minutes. The rationale for each exercise is to isolate and intensify bicep activation promoting muscle growth and strength. Table 7 shows weeks 7-9 of the bicep-focused workout program. It includes four exercises which are the hammer curls, reverse curls, Zottman curls, and Bayesian curls. Load is set at 70% of with 10-12 repetitions and 3-4 sets and an RPE of 7-9. Tempo is 3:0:1 and the rest interval is 3 minutes. The rationale for each exercise is to improve arm strength and muscle mass that targets the brachialis and brachioradialis muscle while also working the biceps and forearms for a upper body workout. Table 8 shows weeks 10-12 of the bicep-focused workout program. It includes four exercises which are the cross-body curls, incline curls, spider curls, and crucifix curls. Load is set at 75% of with 10-12 repetitions and 3-4 sets and an RPE of 8-10. Tempo is 3:0:1 and the rest interval is 3-4 minutes. The rationale for each exercise is to challenge the bicep to do different angles, improving muscle growth, strength and stability. Program Rationale The program starts with an RPE of 3-7 to determine individual effort and volume tolerance. Participants are young male adults; thus, initial progression assumptions are established with caution. The repetition range is intended for hypertrophy (10-20 repetitions) to establish a foundation of muscular endurance and encourage muscle growth while reducing the danger of overtraining and muscle pain. Progressions will be implemented in repetitions, sets, volume, or load, with a focus on perfect form and gradual development. When a participant hits the peak of their prior reps with proper technique, the load will be increased. Set progressions are based on positive adaptation; however, if a 30 participant exhibits poor recovery, sets will be lowered. Sets will only be raised if the target muscle group is not in pain or fatigued prior to the next session. This personalized approach ensures that young adults who are just starting out in fitness is safe and have productive progress. Objectives of the Program This 12-week bicep workout program wanted to achieve considerable bicep muscle growth. The BFR group and the traditional group both followed the same workout routine. The BFR group performed the exercises with bands attached to their biceps. Its purpose was to determine whether BFR bands could stimulate muscular hypertrophy more effectively than usual resistance training. Both groups performed the same repetitions and sets, but the volume increased if the participants could complete the maximum repetitions with proper form. The program also improved muscle endurance. The primary goal was to improve muscle growth and determine what approach was most effective in increasing bicep muscle hypertrophy. 31 Table 5. Week 1 to 3 of the adapted twelve-week mesocycle training program WEEK 1-3 Name of Exercise One Arm bicep Curl Reps Sets Rest Interval 2-3 minutes Rationale 15 3 Barbell bicep Curl 15 3 2-3 minutes Preacher Curl 20 2 2-3 minutes The Preacher curl is an exercise that isolates the biceps brachii. This should serve as the main isolation exercise for the biceps as this locks the elbows on a fixed position. Closed grip barbell bicep curl 15 2 2-3 minutes Close-grip bicep curls target the inner head of the biceps, emphasizing peak development and total arm thickness, resulting in a more defined and muscular body. The One arm bicep curl is an exercise that biases the biceps brachii muscle. This is done seated as standing variation uses more core, leading to more margin of error. Incorporating this exercise gives the respondents a unilateral basis for the program. The Barbell bicep curl is an exercise that biases the biceps brachii but uses more brachialis. The respondents will use the straight bar as this gives the wrist a more supinated position, giving the biceps more peak contraction. This exercise serves as a foundational basis for the program as this is also a compound exercise 32 Table 6. Adapted twelve-week mesocycle training program week 4 to 6 WEEK 4-6 Name of Exercise Concentration curl Reps Sets Rationale 10 4 10 4 3-4 minutes Compared to typical free-weight curls, cable bicep curls provide a better stimulus for muscle growth and strength development by maintaining continuous tension throughout the whole range of action. 12 3 3-4 minutes Seated bicep curls isolate the biceps by reducing motion and allowing for a more focused contraction, resulting in higher muscle activation and a stronger mind-muscle connection. 10 3 3-4 minutes Barbell preacher curls are a targeted and intensive isolation exercise for the biceps that eliminates momentum while optimizing muscle activation, resulting in considerable strength and growth increases. Cable bicep curl Seated bicep curl Barbell preacher curl Rest Interval 3-4 minutes Concentration curls isolate the biceps, pushing them to work harder, resulting in enhanced muscular growth and strength, especially in the inner head. 33 Table 7. Adapted twelve-week mesocycle training program week 7 to 9 WEEK 7-9 Name of Exercise Reps Sets Rest Interval Hammer curls 10 4 3 minutes Reverse curls 10 4 3 minutes Zottman curls 12 3 3 minutes Bayesian curl 10 3 3 minutes Rationale Hammer curls are an efficient exercise for improving arm strength and muscle mass, specifically targeting the brachialis and brachioradialis muscles, while also working the biceps and forearms for a well-rounded upper body workout. Reverse curl is an effective exercise for targeting the brachialis and brachioradialis muscles, leading to forearm strength and hypertrophy while also working the biceps and core stabilizers for a total upper body workout. Zottman curls are a flexible exercise that targets the biceps and forearms, increasing muscular growth and strength, while also engaging the core and shoulder stabilizers for a full upper-body workout. . Bayesian bicep curls have a distinct angle and resistance curve, giving a continual challenge to the biceps, resulting in greater muscular activation, strength, and size development. 34 Table 8. Adapted twelve-week mesocycle training program week 10 to 12 WEEK 10-12 Name of Exercise Cross body curls Reps Sets Rest Interval Rationale 10 4 3-4 minutes Incline curls 10 4 3-4 minutes Spider curls 12 3 3-4 minutes Crucifix curl 10 3 3-4 minutes Cross body curls are a difficult workout for the biceps because they are performed from an unconventional angle that improves balance and muscle growth. Incline curls provide a unique angle for targeting the biceps, allowing for a deeper stretch and contraction, which results in improved muscular activation and growth. Spider curls are an intense isolation exercise for the biceps that eliminates momentum while optimizing muscle engagement, resulting in considerable strength and growth improvements. Crucifix curls are a unique challenge for the biceps, controlling them from an unusual angle, developing balanced muscle development and improving shoulder stability. 35 Program Assessment It was shown that BFR training could induce hypertrophy in trained individuals. It could be assumed that using BFR for arms could help increase muscle size of the biceps and triceps even in individuals who had been training for several years (Dvorak, 2024). BFR could be added into an athlete's regular training regimen to help them build a well-rounded physique or strengthen their arms. Although BFR training was beneficial when done alone, its effects might be enhanced when combined with another resistance training regimen - which was done given the program set in this study. Compared to standard high-rep training, BFR training recruited more muscle fibers. This implied that a significant amount of muscle growth with each set could be encouraged (Duquette & Walker-Ng, 2024). The program was validated by an expert. Program Application BFR, for instance, can be applied to bicep curls. To restrict blood flow to the working muscle (the bicep) during this exercise, a cuff would be placed and tightened around the proximal portion of the arm, which is closest to the body. This allows one to workout with fewer weights and still reap the same benefits of strength and hypertrophy as training with greater loads (Loenneke, 2022). Having them too loose can be preferable to having them too tight. When beginning BFR training, one should err on the side of caution. If skin gets red, that means that the bands are tight enough. One's hands and forearms' vascularity will become more apparent, and pulse can still be detected in the wrist (Dvorak, 2024). 36 BFR Application BFR allows individuals to experience hypertrophy (muscle growth) with significantly lighter weights, typically 20-30% of one-repetition max (1RM), compared to the traditional recommendation of 70-85% 1RM. The restricted blood flow creates a hypoxic (low oxygen) environment in the muscle, stimulating metabolic stress and muscle fiber recruitment similar to heavy resistance training. BFR bears a significant part and a great application to traditional training due to its capability to increase hypertrophic stimulus in lighter resistances. The current program adds a BFR routine alongside the participants’ own programs. Bicep Circumference The researchers measured each participant's mid-upper arm circumference. For gathering measurements, the researchers used a measuring tape. The participant's arm was bare to avoid disrupting the measurement. Participants were relaxed and comfortable when measuring their mid-upper arm circumference. The researchers then identified and positioned the measuring tape halfway between the acromion and olecranon process. The researchers measured the mid-upper arm in centimeters for the pre-test, as it served as the basis, and the post-test after the workout program was implemented to measure the results. 37 Data Analysis Along with the nature of the study, the researchers used a quantitativeexperimental data analysis. To assess the effects of blood flow restriction training on bicep muscle hypertrophy in young male adults, the T-test was used to compare before and after measurements of the mid-upper arm. This test is most appropriate for assessing two groups. The impact of blood flow restriction training (BFR) was assessed using ANCOVA, which accounted for any confounding variables such as baseline muscle size with covariate such as volume of training. Accounting for these characteristics allows ANCOVA to provide a more precise assessment of the individual effect of BFR Training on bicep hypertrophy, resulting in strengthening the study's internal validity and increasing the findings' generalizability. The information gathered was evaluated using ANCOVA to evaluate the post-training results and determine if there was a significant difference in bicep muscle circumference between pre- and post-training measurements. Statistical Treatment of Data The participants of this study are young male adults that are aged above 18 years old. The groups were split into two: BFR group and Conventional group. The participants should have at least 1 year experience in lifting weights. The study conducted had a sample size of 22; (BFR group =12, Conventional group= 10). The BFR group had an average age of 19 ± 0.82 years old and on the other hand, the convention group had an average age of 18.6 ± 0.92 years old. 38 Paired T-test The left and right bicep circumferences of the BFR and conventional groups were compared before and after using a paired t-test. The paired t-test was used to compare the changes in bicep circumference from pre-test to post-test in both the control and experimental groups. The bicep circumference was measured in centimeters both before and after the intervention. This statistical method was used to see if blood flow restriction exercise resulted in a significant increase in bicep muscle growth. ANCOVA This statistical tool was used to check possible difference on the confounding factors of the post-test given that the pre-test measurements were adjusted. The ANCOVA was conducted to determine if there was a significant difference in post-test bicep circumference measurements between the two groups after adjusting for pre-test measurements. This statistical strategy allowed the researcher to look at group differences in the dependent variable after they have adjusted for the covariate. Independent variables (pre-test) The Independent variable/s on the pre-test are the conventional group and the experimental group (BFR). Both the conventional group and experimental group have been given the same program. Moreover, another independent variable 39 is the tightness of the band which has perceived tightness of 7 out of 10. These independent variables are the main components of the study to gather data. Dependent variables (post-test) The dependent variable/s on the post-test are the results from the conducted program both from the conventional and experimental group which would be the right MUAC bicep circumference and the left MUAC bicep circumference. In addition, the intensity is slightly reduced in the BFR group as its own dependent variable for safety standards. Control group (conventional group) The control group in this study is the conventional group with participants who performed a workout program without any interventions. This group did the same workout program as the experimental group to ensure that the volume of the exercise is equal across both groups. The purpose of the conventional group was to be the baseline of comparison allowing the researchers to compare the effects of blood flow restriction training in the experimental group. By being consistent on the workout program, the study aimed to isolate the impact of BFR training on muscle growth and gains. Experimental group (BFR group) The experimental group in this study is the BFR group with participants who did the same workout program as above with added blood flow restriction. This approach applies a BFR bands with a perceived tightness scale of 7 out of 10 40 to bicep muscle during workout is intended to limit blood flow and stimulate muscle growth and gains. The BFR group followed the same workout routine as the control group to ensure that the volume of exercise was equal. The aim was to determine whether BFR training was more successful than traditional training in terms of muscle growth and strength. Confounding variables (volume) To control the variable, both the conventional and experimental groups completed the same workout program. Participants performed the same number of repetitions and sets for each exercise. The weight used in the exercise increased if the participants could complete the maximum number of repetitions while maintaining proper form. The volume was equal in both groups. Because the volume was equal in both groups, there was less bias toward muscular growth. By standardizing the exercise volume, the researchers aimed to isolate the independent variable, which was the experimental group's blood flow restriction. To evaluate the data, two (2) statistical procedures were used, namely, the Paired T-Test and ANCOVA to compare the pre-test and post-test bicep circumference measures and to assess the difference in bicep muscle hypertrophy between the BFR and control group. The Paired t-test was used to compare the means of two samples such as the bicep circumference pre-test and post-test of both groups. The ANCOVA was used to compare the differences in bicep muscle hypertrophy between the two groups while accounting for baseline measures. ANCOVA was used to determine if there was a statistically significant difference 41 between the two groups. This statistical strategy allowed you to look at group differences in the dependent variable after you've adjusted for the covariate. These multiple statistical treatments were critical to the outcomes, and they had proven accurate and reliable. The Paired t-test was utilized in this study to compare the pre-test and posttest bicep circumference measurements within the same participants. This statistical method was appropriate as it accounted for the dependence of observations; each participant’s measurements before and after training were inherently related. The assumptions of the paired t-test included that the differences between the paired observations were normally distributed and that the pairs were randomly and independently selected. Normality was assessed using the Shapiro-Wilk test, which indicated whether the differences were normally distributed (p > 0.05). Results indicated that the study had normally distributed data (p<0.06). The paired t-test was conducted to analyze the changes in bicep circumference from pre-test to posttest for both the control and experimental groups. The bicep circumference measurements were taken in centimeters before the intervention and after completing the training program. These findings suggested that blood flow restriction training resulted in a statistically significant increase in bicep muscle hypertrophy compared to conventional training. The experimental group experienced a greater increase in muscle size, highlighting the effectiveness of BFR training in promoting muscle hypertrophy even at lower weights. The significant results for the experimental group supported the hypothesis that BFR training led to greater bicep hypertrophy compared to conventional training methods. These 42 findings contributed to the understanding of BFR training as a viable option for enhancing muscle growth in young male adults. The ANCOVA was utilized in this study to assess the differences in bicep muscle hypertrophy between the experimental group (Blood Flow Restriction training) and the control group (conventional training) while controlling for baseline measurements. This statistical method allowed for the examination of group differences in the dependent variable (bicep circumference) after adjusting for the covariate (pre-test measurements). The assumptions of ANCOVA were checked to ensure the validity of the analysis. The ANCOVA was conducted to determine if there was a significant difference in post-test bicep circumference measurements between the two groups after adjusting for pre-test measurements. The analysis reported the F-value, degrees of freedom, and p-value for the main effect of the training type. It was anticipated that the ANCOVA revealed a significant effect of training type on bicep muscle hypertrophy. For example, the results indicated that the experimental group demonstrated a greater increase in bicep circumference compared to the control group after controlling for pre-test measurements, with a statistically significant p-value (p < 0.05). Ethical Considerations The researchers provided participants with informed consent forms, PARQ (Physical Activity Readiness Questionnaire), at the start of data collection. By administering the PARQ, the researchers assessed whether participants needed to consult a medical professional before starting the exercise program. This helped 43 ensure participant safety and contributed to the success of the study's objectives. The AHA/ACSM Health/Fitness Facility Pre-participation Screening Questionnaire was designed to check an individual's health status and identify any medical issues or risk factors that may arise during exercise. The researchers also informed the participants of the risks associated with BFR Training such as potential discomfort and vascular stress. Application of BFR cuffs especially to participants that were new to this kind of training may experience difficulty and may feel different compared to the traditional resistance training. Briefing the participants helped them understand what to expect during the period of training and verify the plausible effects of the study. Bicep muscle hypertrophy could be obtained with conventional training. It included lifting weights or doing resistance exercises that work the biceps brachii. Conventional training may cause metabolic stress, which can promote muscular growth. 44 CHAPTER 4 RESULTS AND DISCUSSION In this chapter, the researchers discuss the results and statistics of the findings. The tables used in the statistics below use different methods: Tables (number of tables that will be placed) Table 9. Demographic Profile of Participants sex Average age BFR group Male= 12 19 ± 0.82 Conventional group Male=10 18.6 ± 0.92 Table 9 shows the demographic profile of participants from two groups: the BFR group and the conventional group. The BFR group had 12 males with an average age of 19±0.82, whereas the conventional group had 10 males with an average age of 18.6 ±0.92. This table depicted the gender and age distribution of the participants, giving a basis for interpreting the study's sample demographics. Workout Program The program conducted included movements aimed mainly at hypertrophy through blood flow restriction training. The bicep workout program used in the study included a combination or variation of bicep curls with barbells and dumbbells, as well as isolation exercises such as concentration curls. There were two groups: BFR and conventional groups. The BFR group's primary component was the BFR bands on the mid upper arm, 45 which restricted blood flow. Participants performed in sets of 8-12 repetitions, resting between sets. The load was intentionally lower to enhance stress and muscle fiber recruitment in the BFR group. This approach aimed to cause muscle fatigue and swelling, which would lead to increased muscle growth as compared to typical high-loading exercise. The participants in this 6-week bicep workout program trained twice a week, every other day. This approach was chosen to prioritize recovery between workouts, which is an important aspect in preventing injuries and optimizing muscle repair. By resting their bodies, participants adjusted to the training stimulus, allowing them to progress throughout the program. This frequency aimed to maintain a balance between training sessions and rest days to ensure that participants were not overtrained or injured. Furthermore, twiceweekly bicep exercise provided enough volume and intensity to induce hypertrophy without overwhelming the smaller muscle groups. Biceps recovered faster than larger muscular groups, but they still required enough rest to optimize protein synthesis and reduce fatigue. This frequency also allowed for more scheduling flexibility, making it easier for participants to attend and commit to the program, which is essential to progress. In the study of Kassiano et al. (2025) which compared muscle growth and dynamic strength adaptation found that both methods are effective in promoting bicep hypertrophy. The study emphasized the role of training frequency suggesting that training two times a week can provide sufficient stimulus for muscle growth while allowing enough recovery. Meanwhile, in the study of Lopes da Silva et al. (2024) which compared eccentric and concentric muscle actions on hypertrophy, suggested that eccentric actions are more effective in inducing hypertrophy but noted that the frequency of training played a crucial role in maximizing the effectiveness of eccentric training. 46 The intensity of the workout program was regulated with a rate of perceived exertion (RPE) of 6 and 4 repetitions in reserve (RIR). This method enabled participants to calculate their exertion levels and volume tolerance properly. An RPE of 6 indicated a moderate degree of exertion, whereas an RIR of 4 indicated that the participant should be able to complete four additional repetitions with good form at the end of a set. This approach measured the intensity, allowing participants to adjust in response to changes in strength and fatigue. Using this approach, the program ensured that the participants were exercising within an appropriate range that promoted muscle growth while avoiding injuries and overtraining. The RPE 6 and RIR 4 promoted a controlled and sustainable progression. RPE 6 indicated a moderate challenge ensuring that the muscles underwent stress that is essential to muscle growth. RIR 4 prevented the participants from reaching failure which increased the risk of injury and slowed recovery. This approach also allowed individualization as the participants could adjust the weight used based on their perception of effort and ability to maintain proper form. It was important for the continuous progress throughout the 6-week program as the load gradually increased while the strength improved. Similar studies on producing bicep hypertrophy on the implementation of the program were reported in the study of Kassiano et al (2025) which assessed muscle development and dynamic strength adaptation by performing unilateral and bilateral resistance training. This study showed that planned progression in weight training can result into a significant hypertrophy. While contrasting ideas were also reported in the study of Currier et al. (2022). It was discovered that while higher load and multiple set training were great for strength development, different training prescription improved muscle hypertrophy. This indicated that there were various effective methods for inducing 47 hypertrophy. This meant that there was a gap on the most effective hypertrophy progression approach. This implied that a gap existed in the understanding of how different training variables can influence muscle hypertrophy. Further research needs to explore more on the combined effects of RPE, RIR and other training variables to develop greater guidelines for hypertrophy focus training program. The workout was designed to be performed in three to four sets of 8-12 repetitions per exercise. It was applied to improve muscular hypertrophy and endurance. The range of repetitions allowed the participant to challenge their muscle, and the multiple sets was to ensure the tension of the muscle. This 6-week program aimed to encourage balance by developing muscle growth as well as improving muscle endurance. 8-12 repetitions were an efficient way to promote hypertrophy because they caused metabolic stress and damage to muscles, both of which were necessary for muscular growth. The use of three or four sets increased the stimulus by increasing the time under tension, ensuring that the muscles were sufficiently fatigued. This rep and set method allowed participants to vary the weight used based on their strength and fatigue levels. If the athlete could complete the maximum number of repetitions with proper form, they might increase the weight on the next set. This phase was essential to continue muscular growth. Furthermore, multiple sets help to strengthen participants' muscular endurance, allowing them to endure and work throughout time, thus improving fitness. Similar studies on developing bicep hypertrophy during the program's implementation was provided in Hoseinpour et al.’s (2025) study, which indicates that training 20-30 sets per muscle group per week produced optimal hypertrophy results. Participants who followed this training program experienced significant increases in 48 muscle size and strength over a period of six weeks. While different ideas were also reported, Refalo et al.’s (2024) study, it was found that training to failure was not required for muscle growth and that moderate effort with sufficient volume might produce significant muscular hypertrophy. This indicated that there was a gap in understanding the balance of training volume and intensity for optimal muscle growth. The workout program focused on strength training, specifically for the bicep muscle. Participants participated in a weightlifting exercise to promote muscular hypertrophy. This method utilized resistance training to maximize bicep growth. The study implemented two methods: blood flow restriction training, which used BFR bands to restrict blood flow, and conventional training. This allowed for a comparative analysis of the two-training methods effectiveness on bicep muscle growth of young male adults. Strength and resistance training were the most efficient ways to promote muscle growth. The resistance created enough stress to promote protein synthesis and muscle development. BFR and conventional training allowed for a more holistic approach to bicep development. Conventional training is a solid foundation for maximizing mechanical tension to induce muscle growth. BFR training is a unique metabolic stress since it restricts the blood flow which can induce muscle growth using lighter weights. This comparative analysis is useful for determining which training method is more efficient in developing muscle growth among young male adults. Similar studies on producing bicep hypertrophy on the implementation of the program were reported in the study of by Currier et al. While contrasting ideas were also reported in the study of Krzysztofik et al. (2019) with regard to low load RT under BFR a significant increase in the muscles cross sectional area was observed even without reaching 49 volitional fatigue in particular sets. This implies that a gap in the literature existed, necessitating further research to conclusively determine the most effective training method for bicep hypertrophy. The program started at a perceived exertion (RPE) rate of 6 or 4 repetitions in reserve (RIR) to gauge intensity and volume tolerance. Progression assumptions were made due to a need for previous participant data, especially for participants with less than 3 months of experience. The repetition range aimed for hypertrophy (8–12 reps) to avoid excessive fatigue and muscle damage. Progressions were made in reps, sets, volume, or load. If the participant has reached the maximum repetition range (12), the load was increased. Set progressions were given if the participant adapted well. Still, if they did not, sets were decreased if recovery was poor and only increased if the bicep muscle was not sore nor fatigued for the next session. The systematic progression used in this program was essential for continuous muscular growth. The RPE of 6 and RIR 4 served as a baseline for assessing the participants' current strength and endurance. The 8-12 rep range was intended to optimize hypertrophy while minimizing fatigue and injury. The idea of progressive overload was implemented by increasing the weight when participants could complete 12 repetitions with proper form, ensuring that the muscle was challenged. Set progression was only possible if participants got adequate rest. This individualized progression ensured that participants were safe and might efficiently maximize muscle development in this 6-week program, regardless of their weightlifting experience. Similar studies on producing bicep hypertrophy on the implementation of the program were reported in the study of Kassiano et al (2025) which assessed muscle development and dynamic strength adaptation by performing unilateral and bilateral 50 resistance training. This study showed that planned progression in weight training could result into a significant hypertrophy. While contrasting ideas were also reported in the study of Currier et al. (2022), it was discovered that while higher load and multiple set training are great for strength development, different training prescription improved muscle hypertrophy. This indicated that there were various effective methods for inducing hypertrophy. The exercise's speed was moderate and controlled, which was essential to maximizing muscle engagement while reducing the risk of injury. The pace was set to ensure that the participants maintained consistent muscular tension throughout the whole range of motion. By focusing on controlled movements, participants were able to optimize muscle activation under tension, which lead to muscle growth. The target muscle was the bicep, and the slow and controlled pace allowed for highly concentrated training stimuli, ensuring that the target muscle obtained the most benefits from each repetition that the participants perform. Similar studies on producing bicep hypertrophy on the implementation of the program were reported in the study of Zajac et al. (2021) which said that unintentionally slow tempo could occur during resistance training whereby a heavy load or the manifestation of fatigue was responsible for a slower movement. An intentional slow tempo could be purposefully used when the load was light enough to control and fatigue was not a factor to an individual’s control to the velocity of the movement. While contrasting ideas were also reported in the study of Krzysztofik et al. (2019), saying that faster tempos might be more essential for neural adaptation and strength movements and slower tempos increased time under tension which induce hypertrophy. This implied that a gap about the most optimal tempo for maximizing hypertrophy and further study was 51 needed to determine the effects of different tempos on muscle growth and strength development. This table shows the first three weeks of the twelve-week mesocycle bicep program. It consists of four exercises, specifying the number of repetitions, sets, rest time and targeted muscles for each exercise. The exercises are variations of bicep curls. Table 10. Adapted twelve-week mesocycle training program week 1 to 3 Name of Exercise One Arm Bicep Curl Barbell Bicep Curl Preacher Curl Closed grip barbell bicep curl Reps WEEK 1-3 Sets 15 3 15 20 15 3 2 2 Rest Interval 2-3 minutes Target Muscle Biceps Brachii 2-3 minutes Triceps Brachii Biceps Brachii 2-3 minutes Triceps Brachii Biceps Brachii 2-3 minutes Triceps Brachii Biceps Brachii Brachialis Triceps Brachii 52 Table 11 shows weeks 4-6 of the twelve-week mesocycle program. It includes Concentration Curls, Cable Bicep Curls, Seated Bicep Curls, and Barbell Preacher Curls. The program has lower repetitions and higher sets compared to the previous phase, with longer rest intervals. Table 11. Adapted twelve-week mesocycle training program week 4 to 6 Name of Exercise Reps 10 WEEK 4-6 Sets 4 Rest Interval 3-4 minutes Concentration curl Brachialis 10 4 3-4 minutes Cable bicep curl Triceps Brachii Biceps Brachii Brachialis 12 3 3-4 minutes Seated bicep curl Triceps Brachii Biceps Brachii Brachialis 10 Barbell preacher curl Target Muscle Biceps Brachii 3 3-4 minutes Triceps Brachii Biceps Brachii Brachialis Triceps Brachii 53 Table 12 shows weeks 7–9 of the twelve-week mesocycle program. It consists of Hammer Curls, Reverse Curls, Zottman Curls, and Bayesian Curls. The program consists of 10-12 repetitions, 3-4 sets, and a 3-4 minute rest time, with a focus on strength and muscular development. Table 12. Adapted twelve-week mesocycle training program week 7 to 9 Name of Exercise Reps WEEK 7-9 Sets Rest Interval Target Muscle Biceps Brachii Brachialis Hammer curls 10 4 3 minutes Brachioradialis Triceps Brachii Biceps Brachii Brachialis Reverse curls 10 4 3 minutes Brachioradialis Triceps Brachii Biceps Brachii Brachialis Zottman curls 12 3 3 minutes Brachioradialis Triceps Brachii Biceps Brachii Brachialis Bayesian Curl 10 3 3 minutes Brachioradialis Triceps Brachii 54 Table 13 shows weeks 10 to 12 of the twelve-week mesocycle program. It consists of Cross-body curls, Incline curls, Spider curls, and Crucifix curls. The program consists of 10-12 repetitions, 3-4 sets, and 3-4-minute rest intervals, with a focus on strength and muscular development utilizing various curl movements. Table 13. Adapted twelve-week mesocycle training program week 10 to 12 Name of Exercise Cross body curls Reps 10 WEEK 10-12 Sets 4 Rest Interval 3-4 minutes Target Muscle Biceps Brachii Brachialis Brachioradialis Incline curls 10 4 3-4 minutes Triceps Brachii Biceps Brachii Brachialis Triceps Brachii Spider curls 12 3 3-4 minutes Biceps Brachii Brachialis Crucifix curl 10 3 3-4 minutes Triceps Brachii Biceps Brachii Brachialis Triceps Brachii Changes in Bicep Hypertrophy Between BFR and Conventional Groups Paired-samples t-test was conducted to compare the pre-test of the left bicep circumference for the BFR group (M = 29.17 cm ± 3.20 cm) and post-test results after 6 weeks (M = 32.08 cm ± 3.34 cm). There was a significant difference; t (10) = -3.71, p = 0.009. Also, Paired-samples t-test was conducted to compare the pre- 55 test of the left bicep circumference for the conventional group (M = 29.50 ± 7.88 cm) and post-test results after 6 weeks (M = 30.21 cm ± 8.35 cm). There was a significant difference; t (10) = -2.76, p = 0.022. However, the BFR trained group resulted in a higher percentage increase in muscle size. The BFR group had a higher percentage increase in mid-upper arm circumference than the conventional training group (10.00% vs 3.76%, respectively), with significant differences between pretest and post-test for BFR (p = 0.009) and conventional training (p = 0.022). Table 14 shows that both BFR training and conventional resistance training resulted in significant bicep muscle hypertrophy. Table 14. Comparison of Left Bicep Circumference Changes Between BFR and Conventional Groups BFR group Conventional Group Left MUAC Bicep Circumference (cm) n Before After % p-value Change 12 29.17 ± 32.08 ± 10.00 0.009a 3.20 3.34 10 29.50 ± 30.21 ± 3.76 0.022a 7.88 8.35 Paired-samples t-test was conducted to compare the pre-test of the right bicep circumference for the BFR group (M = 29.20 cm ± 3.51 cm) and post-test results after 6 weeks (M = 31.97 cm ± 3.73 cm). There was a significant difference; t (10) = -2.54, p = 0.027. Also, Paired-samples t-test was conducted to compare the pre-test of the right bicep circumference for the conventional group (M = 30.21 cm ± 8.03 cm) and post-test results after 6 weeks (M = 30.61 cm ± 8.23 cm). There was a significant difference; t (10) = -3.31, p = 0.009. Table 15 shows that both BFR 56 training and conventional resistance training resulted in significant bicep muscle hypertrophy. Table 15. Comparison of Right Bicep Circumference Changes Between BFR and Conventional Groups BFR group Conventional Group Right MUAC Bicep Circumference (cm) n Before After % p-value Change 12 29.20 ± 31.97 ± 9.47 0.027a 3.51 3.73 10 30.21 ± 30.61 ± 2.45 0.009a 8.03 8.23 Table 14 and 15 show that both the BFR and conventional resistance training groups experienced a statistically significant increase in bicep circumference, with the BFR group showed more hypertrophy. The BFR group's right bicep circumference grew by 9.47% (p = 0.027), while the conventional group experienced just a 2.45% rise (p = 0.009). These data support the effectiveness of BFR training as a hypertrophy-enhancing approach, especially for people who struggle with heavier weights. Previous studies suggest that low-load blood flow restriction (BFR) exercise can also promote muscle growth. Ma et al. (2024) showed that BFR training results in muscle thickness comparable to high-load resistance training, supporting its use for individuals who cannot lift heavy weights. Zhang et al. (2023) found that BFR exercise induced hypertrophy in untrained males, indicating its effectiveness in increasing muscular growth through metabolic stress and muscle fiber recruitment. These mechanisms were the same to those seen during high- 57 intensity resistance training, explaining why BFR training remains a very efficient muscle-building mechanism even with low resistance loads. These results suggested that it could be an effective alternative for biceps muscle hypertrophy, especially for those preferring lower mechanical stresses. To get optimal results and maintain security, consider elements such as BFR cuff pressure, proper addition, and identifying indifference. The effectiveness of BFR training in producing muscular hypertrophy could be related to increased metabolic stress and muscle fiber activation, which caused anabolic signaling pathways even at low resistance loads (Zhang et al. (2023). It was discovered that low-load BFR training considerably improved muscle thickness and hypertrophy, indicating its potential as an alternative to standard high-load resistance training. Furthermore, Spranger et al. (2020) showed that metabolic buildup during BFR training specifically lactated and hydrogen ions—played an important role in muscle growth by activating more muscle fibers. This process compensates for the lower mechanical stress encountered in low-load resistance training, resulting in hypertrophic adaptations similar to those reported in high-load training programs (Spranger, 2020). While BFR training has shown better hypertrophy benefits, individual variability, cuff pressure standardization, and exercise selection must all be considered to provide optimal results and participant safety. The study's findings were consistent with current literature, showing that BFR training could be an effective option for muscular growth, particularly in individuals unable to lift heavy 58 loads due to injury or recovery restrictions. Figure 3 shows the changes within the implementation of the bicep hypertrophy program. Figure 3 Comparison of Bicep Circumference Changes Over Time Between BFR and Control Groups Difference between the control and experimental group on bicep circumference The results indicated that there was no significant difference between the left bicep pre-test of the BFR group (M = 29.17 cm ± 3.20 cm) and pre-test of the conventional group (M = 29.50 cm ± 7.88 cm). There was a significant difference; t (10) = -2.62, p = 0.796. Also, the results indicated that there was no significant difference between the right bicep pre-test of the BFR group (M = 29.20 cm, SD = 3.51 cm) and pre-test of the conventional group (M = 31.97 cm ± 8.03 cm). There was a significant difference; t (10) = -0.728, p = 0.475. For posttests, the results indicated that there was no significant difference between the left bicep post-test of the BFR group (M = 32.08 cm ± 3.34 cm) and 59 pre-test of the conventional group (M = 30.21 cm ± 8.35 cm). There was a significant difference; t (10) = 1.028, p = 0.316. The results indicated that there was no significant difference between the right bicep post-test of the BFR group (M = 31.97 cm ± 3.73 cm) and pre-test of the conventional group (M = 30.61 cm ± 8.23 cm). There was a significant difference; t (10) = 0.688, p = 0.500. Table 16. Comparison of Bicep Circumference Changes Between BFR and Conventional Training Groups Bicep circumference (cm) n BFR Group Conventional Group Difference pvalue Left bicep pre-test Right bicep pre-test Left bicep post-test Right bicep posttest 12 10 12 10 29.17 29.20 32.08 31.97 29.50 30.21 30.61 30.95 0.33 1.01 1.47 1.02 0.796 0.475 0.316 0.500 Table 16 compares bicep circumference changes across two training methods: blood flow restriction and conventional training. The table displays the average bicep circumference measured in centimeters for both the left and right arms before and after six weeks of training. The pre-test stage showed that the average bicep circumference was highly similar between the BFR and conventional groups, indicating that they were well-matched. However, there was no significant difference between the two groups. Post-measurement results suggested that the BFR group had higher changes in bicep circumference than the conventional group in both arms. The significance were not observe for the post-test measurements, indicating that the change that was observed did not provide enough evidence to support the hypothesis. While the BFR group had larger improvements in bicep 60 muscle growth, the study was unable to establish that BFR training was superior to conventional training due to a lack of statistical significance. As a result, while the data shows the benefits of BFR training for increasing bicep muscle circumference, the findings were not substantial. Adjusted Difference of Bicep Circumference based on Volume A one-way analysis of covariance (ANCOVA) was conducted to examine the effect of group on the dependent variable while controlling for average volume (Load × Reps × Sets). The overall model was significant, F(2, 21) = 5.495, p = .012, indicating that the predictors collectively explained a significant portion of variance in the dependent variable. The main effect of group was not statistically significant, F(1, 21) = 0.241, p = .628, η² = .007, suggesting that group membership did not significantly impact the dependent variable when controlling for average volume. However, average volume was a significant covariate, F(1, 21) = 10.990, p = .003, η² = .341, indicating a strong relationship between average volume and the dependent variable. Also, a one-way analysis of covariance (ANCOVA) was conducted to examine the effect of group on the dependent variable while controlling for average volume (Load × Reps × Sets). The overall model was significant, F(2, 21) = 7.172, p = .004, indicating that the predictors collectively explained a significant portion of variance in the dependent variable. The main effect of group was not statistically significant, F(1, 21) = 0.675, p = .421, η² = .019, suggesting that group membership did not significantly impact the dependent variable when controlling for average volume. However, average volume was a significant covariate, F(1, 21) = 14.258, 61 p = .001, η² = .397, indicating a strong relationship between average volume and the dependent variable. The adjusted means showed that the effect of hypertrophy using BFR might not have a difference between the control and experimental but had significant effects on the volume of the workout per week. These findings suggested that while group differences were not significant, average volume had a meaningful effect on the outcome for both left and right bicep hypertrophy between the groups. Table 17. Comparison of Post-Test Bicep Circumference and Training Volume Between BFR and Control Groups MUAC Volume (cm) (kgxrepsxset) Post-test Left Bicep BFR group Control Group Post-test BFR group Right Bicep Control Group 30.5 Effect size (η²) 0.003a 0.341b 0.001a 0.397 b 3587 30.5 3465 30.4 3673 30.8 p-value 3447 Note: a: Significant at p<0.05 b: Medium effect size at p<0.05 Although additional examination of the adjusted means suggested that these differences may be primarily influenced by training volume rather than the particular training method, the moderate effect sizes suggested that BFR training contributed to hypertrophic adaptations. This was consistent with earlier research that found that although BFR is useful for increasing muscle mass, when total workload is taken into account, its benefits might be on par with those of 62 conventional resistance training. After adjusting for workout volume, the adjusted means also showed that there was little difference in the hypertrophic responses between the BFR and conventional groups. This implied that rather than the use of BFR per se, the overall training volume might be the main factor influencing muscle growth in both training conditions. Although other studies have demonstrated that BFR is a useful tactic for promoting hypertrophy at lower loads, these results suggested that its benefit might be lessened when training volume was equalized. The importance of progressive overload and total workload in resistance training programs was further supported by the substantial effect that volume has on muscle growth. Hence, optimizing volume may be advantageous for those aiming for hypertrophy whether or not they use BFR. These results add to the increasing amount of research on BFR training's efficacy, especially when compared to traditional resistance training. Dong et al. (2025) said that although BFR has been widely marketed as a low-load alternative for muscle hypertrophy, this study raised the possibility that, when volume was controlled, its advantages may not be appreciably higher than those of conventional techniques. Longer training periods, different BFR protocols, and possible individual variances in BFR training responsiveness should all be investigated in future studies (Miller et al., 2021). Furthermore, Wortman et al. (2020) mentioned that recovery, fatigue management, and viability for various populations, such as athletes and rehabilitation patients, should all be taken into account in real-world BFR applications. 63 According to Ma et al. (2024), BFR training can provide hypertrophic muscle growth comparable to typical higher-load resistance training, but with less resistance. According to Zhang et al. (2023), low-load resistance training with BFR promoted muscle growth in the arms and legs of untrained guys, making it an effective training option for those who cannot carry big weights. These results suggested that BFR exercise can effectively induce hypertrophy, particularly in bicep muscular development in young male adults. Both groups showed muscular growth over six weeks, but statistical analysis showed no difference. However, the findings indicated that, despite identical muscle growth, a significant volume difference may occur between the two methods. It had been observed that the participants' strength had changed more than their hypertrophy. 64 Chapter 5 SUMMARY, CONCLUSIONS, AND RECOMMENDATIONS The summary and findings drawn from the results' analysis and interpretation are presented in this chapter. Additionally, this conveys the conclusions and suggestions that were developed thereafter. Summary This study investigated the effectiveness of blood flow restriction training using BFR bands on increasing bicep muscle hypertrophy compared to the traditional resistance training. The primary objective of this research was to identify the effects of blood flow restriction training on bicep muscle hypertrophy using specialized cuffs specifically made for this kind of training. There are two (2) groups who participated in this study, one group for conventional training (no intervention of BFR Training) and one group for experimental training (with the intervention of BFR Training). These groups underwent certain training sessions with the same training program to assess the muscular hypertrophy on bicep circumference for both control and experimental groups. Up-to-date studies were used as guides in performing the training sessions with and without the use of the BFR bands. The major instrument that was used in gathering data were the training sessions of the control and experimental group as these were designed to seek information and evaluate how effective BFR bands were when used for training programs in terms of the significant differences between the results of the training sessions for the control group and experimental 65 group. In order to collect data, the researchers used experimental procedures. This was carried out in order to gather sufficient and relevant data to meet the study's research objectives. The experiment's collected data was categorized as primary data. The results showed a comparison of both groups before and after training; the BFR group's left bicep circumference was 29.17 to 32.08, and the right arm was 29.20 to 31.97. While in the conventional groups, the left bicep circumference varied from 29.50 to 30.21 and the right arm from 30.21 to 30.61. After six weeks of training, both groups' bicep circumferences increased partially in hypertrophy; the BFR group's left was 30.5 and the right was 30.4, while the conventional group's left was 30.5 and the right was 30.8. However, there was a significant difference based on training volume; for the BFR group, left and right were 3587 and 3673, accordingly, but for conventional training, left and right were 3465 and 3447. According to the findings, BFR training was beneficial for contributing to muscular hypertrophy, but the improvement in muscle strength was considerably noticeable based on the results obtained after the program's duration. Conclusion Blood flow restriction has been considered one of the innovations on improving muscle hypertrophy; however evidence to support the claim on possible increase on bicep hypertrophy is much of a necessity for further study. Both groups experienced muscle growth over the duration of the study, yet the results suggest that despite the similar muscular growth, a possible existence in training volume between the two methods was observed after six weeks. Despite the study's short 66 duration, both groups showed an improvement in muscle hypertrophy and a significant increase in training volume. Factors such as training load and overall workload may vary between BFR and conventional training such that both training methods appear to be effective for muscle growth, allowing individuals to choose based on their preferences and goals. Further application on BFR training should be implemented on programs. Recommendation For BFR application for longer duration of study. The researchers strongly suggest that future research studies on BFR training be conducted for a prolonged period. Extending the study's duration could allow for an improved assessment of BFR's efficiency and risk. Research should also focus on the body's long-term adaptations to muscle hypertrophy, strength, endurance, and safety issues for possible BFR effects. This extended study will provide a better knowledge of the BFR and its benefits in the population. For Strength tests. BFR training has the potential to improve an individual's strength, so future research ought to research into the benefits of BFR training for enhancing muscular strength. It is highly encouraged that researchers conduct more research on BFR training for strength improvement as an alternative to typical resistance training methods. This suggestion was to determine BFR parameters for strength development, such as cuff pressure, volume, load, and frequency. Exploring BFR training in this type of training has the potential to increase strength training for people of different backgrounds. 67 For young male adults. For young male adults looking to improve their bicep muscle growth. Both approaches may result in muscle hypertrophy, and BFR training can be used if individuals wish to lift smaller weights, are still recovering from an injury, or simply want to vary their workouts. Things to consider while adopting BFR training include proper technique and cuff pressure for their safety. Regardless of the method they choose consistency and dedication to the program are essential for muscular growth. For future researchers. Researchers who want to expand on this study of BFR training for bicep muscle hypertrophy can look into the training volume and load of BFR training compared conventional training. Studies may focus on the repetitions, load, sets, and recovery time of an individual who applies both methods. Additional factors to consider are the pressure of the BFR that will be applied, the bands that will be utilized, and the workout selections, which may improve the data that will be collected. Furthermore, extending the activity for more than six weeks can help to analyze the long-term effects of an individual's potential muscle growth. The demographics of the participants can also influence the generalizability of the results. Finally, integrating advance measuring techniques could provide more precise assessments on the muscle hypertrophy and underlying physiological mechanisms. 68 References Androulakis-Korakakis, P., Wolf, M., Coleman, M., Burke, R., Piñero, A., Nippard, J., & Schöenfeld, B. J. (2023). Optimizing Resistance Training Technique to Maximize Muscle Hypertrophy: A Narrative Review. Journal of Functional Morphology and Kinesiology, 9(1), 9–9. https://doi.org/10.3390/jfmk9010009 Balachandran, A. T., Steele, J., Angielczyk, D., Belio, M., Schoenfeld, B. J., Quiles, N., Askin, N., & Abou-Setta, A. M. (2022). Comparison of Power Training vs Traditional Strength Training on Physical Function in Older Adults. JAMA Network Open, 5(5), e2211623. https://doi.org/10.1001/jamanetworkopen.2022.11623 Chang, H., Zhang, J., Yan, J., Yang, X., Chen, B., & Zhang, J. (2024). Effects of Blood Flow Restriction Training on Muscle Strength and Hypertrophy in Untrained Males: A Systematic Review and Meta-Analysis Based on a Comparison with High-Load Resistance Training. Life, 14(11), 1442. https://doi.org/10.3390/life14111442 Currier, B. S., Mcleod, J. C., Banfield, L., Beyene, J., Welton, N. J., D’Souza, A. C., Keogh, J. A. J., Lin, L., Coletta, G., Yang, A., Colenso-Semple, L., Lau, K. J., Verboom, A., & Phillips,1 S. M. (2023). Resistance training prescription for muscle strength and hypertrophy in healthy adults: a systematic review and Bayesian network meta-analysis. British Journal of Sports Medicine,2 57(18). https://doi.org/10.1136/bjsports-2023-106807 69 da Silva, L. S. L., Gonçalves, L. D. S., Alves Campos, P. H., Benjamim, C. J. R., Tasinafo Júnior, M. F., de Lima, L. C. R., Bueno Júnior, C. R., & Alves, C. P. L. (2025). Comparison Between Eccentric vs. Concentric Muscle Actions On Hypertrophy: A Systematic Review and Meta-analysis. Journal of strength and conditioning research, 39(1), 115–134. https://doi.org/10.1519/JSC.0000000000004981 Dong, K., Tang, J., Xu, C., Gui, W., Tian, J., Chun, B., Li, D., & Wang, L. (2025). The effects of blood flow restriction combined with endurance training on athletes’ aerobic capacity, lower limb muscle strength, anaerobic power and sports performance: a meta-analysis. BMC Sports Science Medicine and Rehabilitation, 17(1). https://doi.org/10.1186/s13102-025-01072-y Rockhill, M., Early, K. S., Bryan, A., Tyo, B., Buuck, D., & McGinty, J. (2020). Effect of blood flow restriction training on muscular performance, pain and vascular function.3 International Journal of Sports Physical Therapy, 15(6), 892–900.4 https://doi.org/10.26603/ijspt20200892 Grønfeldt, B. M., Lindberg Nielsen, J., Mieritz, R. M., Lund, H., & Aagaard, P. (2020). Effect of blood‐flow restricted vs heavy‐load strength training on muscle strength: Systematic review and meta‐analysis. Scandinavian Journal of Medicine & Science in Sports,5 30(5). https://doi.org/10.1111/sms.13632 Hernández-Martín, A., et al. (2024). Blood flow restriction training and muscle adaptations: A Meta-Analysis. Physiology.https://doi.org/10.3389/fphys.2024.00112 Frontiers in 70 Hernández-Martín, M., Plaza-Manzano, G., & López-de-Uralde-Villanueva, I. (2024). Effects of low-load blood flow restriction training on muscle anabolism biomarkers compared with traditional training in healthy adults. MDPI. https://doi.org/10.3390/life14030411 Johnson, A. M., & Sandage, M. J. (2021). Exercise Science and the Vocalist. Journal of Voice, 35(4), 668–677. https://doi.org/10.1016/j.jvoice.2021.06.029 Jørgensen, S. L., Kierkegaard-Brøchner, S., Marie Bagger Bohn, Mathias Høgsholt, Aagaard, P., & Mechlenburg, I. (2023b). Effects of blood-flow restricted exercise versus conventional resistance training in musculoskeletal disorders—a systematic review and meta-analysis. BMC Sports Science, Medicine & Rehabilitation, 15(1). Kassiano, W. Nunes, J. P., Costa, B., Ribeiro, A. S., Loenneke, J. P., & Cyrino, E. S. (2025). Comparison of Muscle Growth and Dynamic Strength Adaptations Induced by Unilateral and Bilateral Resistance Training: A Systematic Review and Meta-analysis. Sports Medicine. https://doi.org/10.1007/s40279-024-02169-z Krzysztofik, M., Wilk, M., Wojdała, G., & Gołaś, A. (2019). Maximizing muscle hypertrophy: a systematic review of advanced resistance training techniques and methods.6 International Journal of Environmental Research and Public Health, 16(24),7 E4897. https://doi.org/10.3390/ijerph16244897 Lorenz, D., Bailey, L., Wilk, K., Mangine, B., Head, P., Grindstaff, T. L., & Morrison, S. (2021). Current Clinical Concepts: Blood Flow Restriction Training. Journal of Athletic Training, 56(9). https://doi.org/10.4085/418-20 71 Ma, F., He, J., & Wang, Y. (2024). Blood flow restriction combined with resistance training on muscle strength and thickness improvement in young adults: a systematic review, meta-analysis, and meta-regression. Frontiers in Physiology, 15, 1379605. https://doi.org/10.3389/fphys.2024.1379605 Markus Estifanos Haugen, Fredrik Tonstad Vårvik, Larsen, S., Arvid Steinar Haugen, Roland, & Bjørnsen, T. (2023). Effect of free-weight vs. machine-based strength training on maximal strength, hypertrophy and jump performance – a systematic review and meta-analysis. BMC Sports Science, Medicine & Rehabilitation, 15(1). https://doi.org/10.1186/s13102-023-00713-4 Miller, B. C., Tirko, A. W., Shipe, J. M., Sumeriski, O. R., & Moran, K. (2021). The Systemic Effects of Blood Flow Restriction Training: A Systematic Review. International Journal of Sports Physical Therapy, 16(4), 978–990.8 https://doi.org/10.26603/001c.25791 Nejatian Hoseinpour, A., Bassami, M., Ahmadizad, S., Donath, L., Setayesh, S., Mirzaei, M., & Mohammad Rahimi, G. R. (2025). The influence of resistance training on inflammatory markers, body composition and functional capacity in healthy older adults: A systematic review and metaanalysis. Archives of Gerontology and Geriatrics, 130, 105731. https://doi.org/10.1016/j.archger.2024.105731 Nyakayiru, J., Fuchs, C. J., Trommelen, J., Smeets, J. S. J., Senden, J. M., Gijsen, A. P., Zorenc, A. H., Van Loon, L. J. C., & Verdijk, L. B. (2019). Blood Flow Restriction Only Increases Myofibrillar Protein Synthesis with Exercise. 72 Medicine and Science in Sports and Exercise,9 51(6), 1137–1145. https://doi.org/10.1249/MSS.0000000000001899 Patterson, S. D., Hughes, L., Warmington, S., Burr, J., Scott, B. R., Owens, J., Abe, T., Nielsen, J. L., Libardi, C. A., Laurentino, G., Neto, G. R., Brandner, C., Martin-Hernandez, J., & Loenneke, J. (2019). Blood Flow Restriction Exercise: Considerations of Methodology, Application,12 and Safety. Frontiers in Physiology, 10(533).13 https://doi.org/10.3389/fphys.2019.00533 Pignanelli, C., Christiansen, D., & Burr, J. F. (2021). Blood flow restriction training and the high-performance athlete: science to application. Journal of Applied Physiology, 130(4). https://doi.org/10.1152/japplphysiol.00982.2020 Pignanelli, C., Petrick, H. L., Keyvani, F., Heigenhauser, G. J. F., Quadrilatero, J., Holloway, G. P., & Burr, J. F. (2020). Low-load resistance training to task failure with and without blood flow restriction: muscular functional and structural adaptations. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 318(2), R284–R295. https://doi.org/10.1152/ajpregu.00243.2019 Refalo, M. C., Helms, E. R., Robinson, Z. P., D. Lee Hamilton, & Fyfe, J. J. (2024). Similar muscle hypertrophy following eight weeks of resistance training to momentary muscular failure or with repetitions-in-reserve in resistancetrained individuals. Journal of Sports https://doi.org/10.1080/02640414.2024.2321021 Sciences, 42(1), 1–17. 73 Sieljacks, P., et al. (2019). BFR Training and Muscle Protein Synthesis: A Comparative Study. Journal of Strength and Conditioning Research, 33(4), 887-895. https://doi.org/10.1519/JSC.0000000000002989 Spranger, M. D. (2020). Commentary: Blood Flow Restriction Exercise: Considerations of Methodology, Application, and Safety. Frontiers in Physiology, 11. https://doi.org/10.3389/fphys.2020.599592 Wortman, R. J., Brown, S. M., Savage-Elliott, I., Finley, Z. J., & Mulcahey, M. K. (2020). Blood Flow Restriction Training for Athletes: A Systematic Review. The American Journal of Sports Medicine, 49(7), 363546520964454. https://doi.org/10.1177/0363546520964454 Zajac, A., Wilk, M., & Tufano, J. J. (2021). The Influence of Movement Tempo during Resistance Training on Muscular Strength and Hypertrophy Responses: a Review. Sports Medicine (Auckland, N.Z.), 51(8). https://doi.org/10.1007/s40279-021-01465-2 74 APPENDICES APPENDIX A – High Performance Gym Request Letter November 21, 2024 Ms. Elcy C. Lacambra Administration Director NU Laguna Km. 53 Pan-Philippine Highway, Calamba, Philippines 4028 Dear Ms. Lacambra, Greetings in Gold and Blue! We, the researchers from the School of Arts and Sciences, Exercise and Sports Science program, are conducting a study titled “The Effects of Blood Flow Restriction Training on Bicep Muscle Hypertrophy among Young Male Adults”. In partial fulfillment of the requirements for the Degree of Bachelor of Science in Exercise and Sport Sciences. As part of this research, we respectfully request permission to utilize the HighPerformance Gym facilities on the following dates from around 7:00 AM to 6:00 PM: • Monday: November 25; December 2, 9, 16; January 6, 13, 20, 27 (8 days total) • Tuesday: November 26; December 3, 10, 17; January 7,14,21,28 (8 days total) • Wednesday: November 27; December 4, 11, 18; January 8, 15, 22, 29 (8 days total) • Thursday: November 28, December 5,12,19; January 9, 16, 23, 30 (8 days total) • Friday: November 29; December 6, 13, 20; January 10, 17, 24, 31 (8 days total) Our study involves 22 respondents who are NU Laguna students (names to follow). The gym provides access to reliable and complete equipment essential for 75 our research. We selected these days while considering participant availability and class scheduling into consideration. We sincerely hope for your approval and support in this research, as this study contributes to advancing knowledge of training techniques, raise awareness of the importance of fitness and health and its applications in exercise science. Should you grant our request, please sign below to formalize your approval. Thank you for considering our request. We are grateful for your assistance in facilitating our research. Respectfully yours, USHLEE ENRIQUE A. BASAS Researcher 1, Bachelor of Science in Exercise and Sports Sciences ELAINE JAIRUSSE R. CAPELLAN Researcher 2, Bachelor of Science in Exercise and Sports Sciences YUEN MAGDALENNA WILLIAM VERBRUGGEN Researcher 3, Bachelor of Science in Exercise and Sports Sciences Noted By: ERICK VOLTAIRE P. TABING, RND, MSApN, CPSS, CPT, CSCC Research Adviser MILPERT JOHN B. MAROTO, LPT, MPES, CPT Program Chair, Bachelor of Science in Exercise and Sports Sciences 76 Reviewed By: Approved by: NEILSON A. SILVA, LPT, MAEd Dean & Adviser, School of Arts and Sciences Ms. ELCY C. LACAMBRA Administration Director – NU Laguna 77 APPENDIX B Informed Consent for Bicep Muscle Hypertrophy Training Participants We are fourth-year students at National University Laguna, pursuing a Bachelor of Science in Exercise and Sports Science with a focus on Fitness and Sports Coaching. As part of our final research requirement, "The Effects of Blood Flow Restriction Training on Bicep Muscle Hypertrophy among Young Male Adults," we are undertaking a study to determine the effectiveness of blood flow restriction (BFR) exercise in stimulating muscle growth. This study seeks to investigate the physiological responses and practical uses of BFR training, with a particular emphasis on its effect on bicep muscle hypertrophy in young male adults. By investigating this unique training technique, we aim to add to the academic and professional body of knowledge in sports science, particularly in the areas of strength and conditioning and muscle development. This study intends to track and gather data on the physiological responses of young male adults undergoing blood flow restriction (BFR) training, with a particular emphasis on bicep muscle hypertrophy. This study aims to highlight the value of BFR training as a method for increasing muscle growth by thoroughly monitoring and assessing changes in muscle size and related factors. This study will serve to promote evidence-based practices in strength and conditioning, as well as give significant findings for aspiring sports coaches, personal trainers, and exercise science professionals, particularly in terms of the use of BFR training for targeted muscle development. Through this study, young male adults who participate in resistance training could get a better understanding of the physiological effects of blood flow restriction (BFR) on muscle growth. 78 Participants can learn about the exact characteristics and applications of BFR training that enhance bicep muscle growth by analyzing the data acquired. Furthermore, the findings will add to the academic field of sports science by giving evidence-based information that could help other individuals and professionals looking to use BFR training as a technique of improving muscular growth and strength. This study is intended to pose minimal risk of injury to participants. Before participating, all individuals will be thoroughly screened to ensure they match the inclusion criteria and have no contraindications to blood flow restriction (BFR) training. All BFR sessions will be done under the supervision of the researchers, in accordance with recognized safety guidelines. Participants will be continuously watched for any adverse responses, and the BFR pressure will be adjusted as appropriate to maintain comfort and safety. If any pain or concerns occur, participants may stop the session and withdraw from the study at any time. Support resources, including access to medical professionals, will be made accessible as necessary. Contact Information If you have any questions, concerns, or wish to withdraw please contact: basasua@students.nu-laguna.edu.ph capellaner@students.nu-laguna.edu.ph verbruggeny@students.nu-laguna.edu.ph Consent I have read and understand the information provided in this Informed Consent Form. I understand the purpose of the “Research” the potential risks associated with it, and my responsibilities as a participant. I also understand that I have the right to withdraw from the program at any time without penalty. Participant's Full Name: ___________________________ Participant's Signature: ___________________________ Date: ___________________________ 79 APPENDIX C The Six Week Mesocycle Program WEEK 1-3 Name of Exercise One Arm Bicep Curl Target Muscles Agonist Antagonist Biceps Brachii Triceps Brachii Dynamic Stabilizer Supraspinatus Infraspinatus Static Stabilizer Rectus abdominis Obliques Wrist flexors/ extensors Erector spinae Load Reps Sets RPE Tempo Rest Interval 40% 15 3 3-7 3:0:1:0 2-3 minutes The One arm Bicep curl is an exercise that biases the biceps brachii muscle. This is done seated as standing variation uses more core, leading to more margin of error. Incorporating this exercise gives the respondents a unilateral basis for the program. 40% 15 3 3-7 3:0:1:0 2-3 minutes The Barbell bicep curl is an exercise that biases the biceps brachii, but uses more brachialis. The repondents will use the straight-bar as this gives the wrist a more supinated position, giving the biceps more peak contraction. This exercise serves as a foundational basis for the program as this is also a compound exercise The Preacher curl is an exercise that isolates the biceps brachii. This should serve as the main isolation exercise for the biceps as this locks the elbows on a fixed position. Close-grip bicep curls target the inner head of the biceps, emphasizing peak development and total arm thickness, resulting in a more defined and muscular body. Rationale Trapezius Barbell Bicep Curl Biceps Brachii Triceps Brachii Brachialis Rhomboids Brachialis Preacher Curl Biceps Brachii Triceps Brachii Anterior Deltoids Anterior Deltoids 40% 20 2 3-7 3:0:1:0 2-3 minutes Closed grip barbell bicep curl Biceps Brachii Triceps Brachii Supraspinatus Infraspinatus Rectus abdominis Obliques 40% 15 2 3-7 3:0:1:0 2-3 minutes Brachialis Wrist flexors/ extensors Erector spinae 80 WEEK 4-6 Name of Exercise Target Muscles Agonist Antagonist Dynamic Stabilizer Supraspinatus Concentration curl Biceps Brachii Triceps Brachii Brachialis Infraspinatus Wrist flexors/ extensor Supraspinatus Cable bicep curl Biceps Brachii Triceps Brachii Brachialis Infraspinatus Wrist flexors/ extensor Supraspinatus Seated bicep curl Biceps Brachii Triceps Brachii Brachialis Infraspinatus Wrist flexors/ extensor Supraspinatus Barbell preacher curl Biceps Brachii Brachialis Triceps Brachii Infraspinatus Wrist flexors/ extensor Load Reps Sets RPE Tempo Rest Interval Static Stabilizer Rectus abdominis Obliques 60% 12 4 4-8 3:0:1:0 2-3 minutes Trapezius Rhomboids Rectus abdominis Obliques 60% 12 4 4-8 3:0:1:0 2-3 minutes Trapezius Rhomboids Rectus abdominis Obliques 60% 15 3 4-8 3:0:1:0 2-3 minutes Trapezius Rhomboids Rectus abdominis Obliques Erector spinae Trapezius Rhomboids 60% 15 3 4-8 3:0:1:0 2-3 minutes Rationale Concentration curls isolate the biceps, pushing them to work harder, resulting in enhanced muscular growth and strength, especially in the inner head. Compared to typical free-weight curls, cable bicep curls provide a better stimulus for muscle growth and strength development by maintaining continuous tension throughout the whole range of action. Seated bicep curls isolate the biceps by reducing motion and allowing for a more focused contraction, resulting in higher muscle activation and a stronger mindmuscle connection. Barbell preacher curls are a targeted and intensive isolation exercise for the biceps that eliminates momentum while optimizing muscle activation, resulting in considerable strength and growth increases. 81 WEEK 7-9 Name of Exercise Target Muscles Agonist Antagonist Dynamic Stabilizer Biceps Brachii Hammer curls Brachialis Triceps Brachii Brachioradialis Brachioradialis Brachialis Reps Sets RPE Tempo Rest Interval Rationale 3 minutes Hammer curls are an efficient exercise for improving arm strength and muscle mass, specifically targeting the brachialis and brachioradialis muscles, while also working the biceps and forearms for a well-rounded upper body workout. 3 minutes Reverse curl is an effective exercise for targeting the brachialis and brachioradialis muscles, leading to forearm strength and hypertrophy while also working the biceps and core stabilizers for a total upper body workout. 3 minutes Zottman curls are a flexible exercise that targets the biceps and forearms, increasing muscular growth and strength, while also engaging the core and shoulder stabilizers for a full upperbody workout. . Static Stabilizer Rectus abdominis Obliques Erector spinae 70% 10 4 7-9 3:0:1:0 Trapezius Rhomboids Rectus abdominis Biceps Brachii xReverse curls Load Triceps Brachii Supraspinatus Infraspinatus Brachioradialis Obliques 70% 10 4 7-9 3:0:1:0 Trapezius Rhomboids Supraspinatus Biceps Brachii Zottman curls Brachialis Infraspinatus Triceps Brachii Brachioradialis Extensor carpi radialis Rectus abdominis 70% 12 3 7-9 3:0:1:0 Obliques Ulnaris Biceps Brachii Bayesian curl Brachialis Brachioradialis Triceps Brachii Supraspinatus Rectus abdominis Infraspinatus Obliques Wrist flexors/ extensor Trapezius Rhomboids 70% 10 3 7-9 3:0:1:0 3 minutes Bayesian bicep curls have a distinct angle and resistance curve, giving a continual challenge to the biceps, resulting in greater muscular activation, strength, and size development. 82 WEEK 10-12 Name of Exercise Cross body curls Target Muscles Agonist Antagonist Biceps Brachii Triceps Brachii Brachialis Incline curls Brachioradialis Biceps Brachii Infraspinatus Triceps Brachii Brachialis Spider curls Biceps Brachii Dynamic Stabilizer Supraspinatus Supraspinatus Infraspinatus Wrist flexors/extensors Triceps Brachii Brachialis Supraspinatus Infraspinatus Wrist flexors/extensors Crucifix curl Biceps Brachii Brachialis Triceps Brachii Supraspinatus Static Stabilizer Rectus abdominis Obliques Rectus abdominis Obliques Reps Sets RPE Tempo Rest Interval 75% 10 4 8-10 3:0:1:0 3-4 minutes Cross body curls are a difficult workout for the biceps because they are performed from an unconventional angle that improves balance and muscle growth. 75% 10 4 8-10 3:0:1:0 3-4 minutes Incline curls provide a unique angle for targeting the biceps, allowing for a deeper stretch and contraction, which results in improved muscular activation and growth. 75% 12 3 8-10 3:0:1:0 3-4 minutes Spider curls are an intense isolation exercise for the biceps that eliminates momentum while optimizing muscle engagement, resulting in considerable strength and growth improvements. 75% 10 3 8-10 3:0:1:0 3-4 minutes Crucifix curls are a unique challenge for the biceps, controlling them from an unusual angle, developing balanced muscle development and improving shoulder stability. Rationale Erector spinae Trapezius Rhomboids Rectus abdominis Obliques Erector spinae Trapezius Rhomboids Rectus abdominis Infraspinatus Obliques Wrist flexors/ extensor Load Trapezius Rhomboids 83 Program Rationale The program starts with an RPE of 3-7 to determine individual effort and volume tolerance. Participants are young male adults, thus initial progression assumptions are established with caution.The repetition range is intended for hypertrophy (10-20 repetitions) to establish a foundation of muscular endurance and encourage muscle growth while reducing the danger of overtraining and muscle pain. Progressions will be implemented in repetitions, sets, volume, or load, with a focus on perfect form and gradual development. When a participant hits the peak of their prior reps with proper technique, the load will be increased.Set progressions are based on positive adaptation; however, if a participant exhibits poor recovery, sets will be lowered. Sets will only be raised if the target muscle group is not painful or fatigued prior to the next session. This personalized approach ensures that young adults who are just starting out in fitness is safe and have productive progress. 84 APPENDIX D- CERTIFICATE OF STATISTICIAN CERTIFICATE OF STATISTICIAN This is to certify that the research entitled “THE EFFECTS OF BLOOD FLOW RESTRICTION TRAINING ON BICEP MUSCLEHYPERTROPHY AMONG YOUNG MALE ADULTS by Ushlee Enrique A. Basas, Elaine Jairusse R. Capellan and Yuen Magdalena William Verbruggen, had been checked and statistically corrected by the undersigned. CERTIFIED BY: Angelica M. Tabing Statistician 85 86 APPENDIX CURRICULUM VITAE Elaine Jairusse R. Capellan elainejairussecapellan@gmail.com | 09053369245 PERSONAL INFORMATION Date of Birth: July 21, 2003 Age: 21 years old Place of Birth: Manila City Height: 5’3 Religion: Islam Sex: Female Civil Status: Single EDUCATIONAL ATTAINMENT Bachelor’s Degree: 2022-Present National University (NU Laguna) Bachelor of Science in Exercise and Sports Science Major in Fitness and Coaching 87 CURRICULUM VITAE Yuen Magdalena William Verbruggen yuenverbruggen@gmail.com | 09565635804 PERSONAL INFORMATION Date of Birth: January 12, 2003 Age: 22 years old Place of Birth: Belgium Height: 6’0 Religion: Catholic Sex: Male Civil Status: Single EDUCATIONAL ATTAINMENT Bachelor’s Degree: 2021-Present National University (NU Laguna) Bachelor of Science in Exercise and Sports Science Major in Fitness and Coaching 88 CURRICULUM VITAE Ushlee Enrique Basas shlnrq6@gmail.com | 09632078554 PERSONAL INFORMATION Date of Birth: November 9,2002 Age: 22 years old Place of Birth: Calamba, Laguna Height: 5’7 Religion: Catholic Sex: Male Civil Status: Single EDUCATIONAL ATTAINMENT Bachelor’s Degree: 2021-Present National University (NU Laguna) Bachelor of Science in Exercise and Sports Science Major in Fitness and Coaching
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