FULL NAME OF TEACHER-SCHOLAR: LASYAM A. QUISALAN SCHOOL ASSIGNMENT: CANATUAN NATIONAL HIGH SCHOOL PHONE NUMBER: 09673045439 Pretest Essay Direction: Answer what is being asked in the situations below. 1. Three of five patients who underwent heart valve replacement surgery developed bacteremia. The causative agent was Enterobacter cloacae. What were the patients’ signs and symptoms? (give 2 very significant symptoms) How would you identify this bacterium? (suggest 1 very effective method). A manometer used in the operations was culture-positive for E. cloacae. What is the most likely source of this contaminant? (give 1 valid contaminant) Suggest 1 way of preventing such occurrences. (5 pts) 1. Signs and Symptoms of Bacteremia due to Enterobacter cloacae: • • Fever: One of the most common signs of bacteremia due to any bacterial infection. Hypotension: Due to the systemic infection and possible septic shock, patients often experience low blood pressure, especially as the infection worsens. 2. How to Identify Enterobacter cloacae (Effective Method): • Biochemical testing (e.g., Enterotube or API 20E): This is a very effective and commonly used method for identifying Enterobacter cloacae. The bacterium can be identified by its characteristic fermentation pattern (e.g., lactose fermentation) and other biochemical reactions in commercial identification systems. 3. Most Likely Source of Contaminant: • Contaminated medical equipment: Since the manometer (a piece of surgical equipment) was culture-positive for E. cloacae, it is likely that the bacterium was introduced via improperly sterilized or handled medical equipment during surgery. 4. Prevention Method: • Strict sterilization protocols: Ensuring that all medical instruments, especially those that come into direct contact with sterile body tissues (e.g., heart valves), are properly sterilized between uses. Regular checks and maintenance of sterilization equipment (autoclaves) should be performed to avoid cross-contamination. FULL NAME OF TEACHER-SCHOLAR: LASYAM A. QUISALAN SCHOOL ASSIGNMENT: CANATUAN NATIONAL HIGH SCHOOL PHONE NUMBER: 09673045439 2. List the causative agent/s, symptom/s, and treatment for any four (4) viral diseases of the respiratory system. Separate the diseases according to whether they infect the upper or lower respiratory system. Present your answers in a table form.( 24 pts) Disease Causative Agent(s) Upper Respiratory System 1. Common Cold Rhinovirus, Coronavirus Symptoms Sneezing, runny nose, sore throat, mild cough, congestion, low-grade fever 2. Influenza (Flu) Influenza Virus (A, B, High fever, chills, or C) fatigue, sore throat, cough, muscle aches, headache Lower Respiratory System 3. Respiratory Respiratory Syncytial Wheezing, cough, Syncytial Virus Virus (RSV) fever, difficulty (RSV) Infection breathing, nasal congestion, irritability 4. Pneumonia Influenza Virus, (Viral) Adenovirus, Respiratory Syncytial Virus (RSV) Cough, fever, difficulty breathing, chest pain, fatigue, shortness of breath Treatment Symptomatic treatment: rest, hydration, decongestants, pain relievers Antiviral medications (e.g., oseltamivir), rest, fluids, pain relievers Supportive care (e.g., oxygen therapy, hydration), RSV-specific monoclonal antibody (Palivizumab) for high-risk infants Antiviral medications (e.g., oseltamivir for flu), supportive care (oxygen, fluids) FULL NAME OF TEACHER-SCHOLAR: LASYAM A. QUISALAN SCHOOL ASSIGNMENT: CANATUAN NATIONAL HIGH SCHOOL PHONE NUMBER: 09673045439 Assessment Multiple Choice Directions: Choose the letter of the correct answer. Use the following choices to answer questions 1 through 4: a. ehrlichiosis b. Lyme disease c. septic shock d. toxoplasmosis e. viral hemorrhagic fever E 1. A patient presents with vomiting, diarrhea, and a history of fever and headache. Bacterial cultures of blood, CSF, and stool are negative. What is your diagnosis? A 2. A patient was hospitalized because of continuing fever and progression of symptoms including headache, fatigue, and back pain. Tests for antibodies to Borrelia burgdorferi were negative. What is your diagnosis? D 3. A patient complained of headache. A CT (computed tomography) scan revealed cysts of varying size in her brain. What is your diagnosis? C 4. A patient presents with mental confusion, rapid breathing and heart rate, and low blood pressure. What is your diagnosis? 5. A patient has a red circular rash on his arm and fever, malaise, and joint pain. The most appropriate treatment is a. antibiotics. b. chloroquine. c. anti-inflammatory drugs. d. antimony. Use the following choices to answer questions 6 and 7: a. brucellosis b. malaria c. relapsing fever d. Rocky Mountain spotted fever e. Ebola hemorrhagic fever 6. The patient’s fever spikes each evening. Oxidase-positive, gram negative coccobacilli were isolated from a lesion on his arm. What is your diagnosis? a. brucellosis 7. The patient was hospitalized with fever and headache. Spirochetes were observed in her blood. What is your diagnosis? c. relapsing fever 8. Nineteen workers in a slaughterhouse developed fever and chills, with the fever spiking to 40°C each evening. The most likely method of transmission of this disease is a. a vector. b. the respiratory route. c. a puncture wound. d. an animal bite. 9. A patient has fever, difficulty breathing, chest pains, fluid in the alveoli, and a positive tuberculin skin test. Gram-positive cocci are isolated from the sputum. The recommended treatment is a. fluoroquinolone. b. antitoxin. c. isoniazid. d. tetracyclines. 10. No bacterial pathogen can be isolated from the sputum of a patient with pneumonia. Antibiotic therapy has not been successful. The next step should be a. culturing for Mycobacterium tuberculosis. b. culturing for Mycoplasma pneumoniae. c. culturing for fungi. d. a change in antibiotics. Match the following choices to the culture descriptions in questions 11 through 12: a. Chlamydophila b. Coccidioides c. Histoplasma d. Mycobacterium e. Mycoplasma E 11. Your culture from a pneumonia patient appears not to have grown. You do see colonies, however, when the plate is viewed at 100×. C 12. Microscopic examination of a lung biopsy shows ovoid cells in macrophages. You suspect these are the cause of the patient’s symptoms, but your culture grows a filamentous organism. 13. In San Francisco, ten animal health care technicians developed pneumonia 2 weeks after 130 goats were moved to the animal shelter where they worked. Which of the following is false? a. Diagnosis is made by a blood agar culture of sputum. b. The cause is Coxiella burnetii. c. The bacteria produce endospores. d. The disease was transmitted by aerosols. 14. Which of the following leads to all the rest? a. catarrhal stage b. loss of cilia c. mucus accumulation d. tracheal cytotoxin Match the following choices to the statements in questions 15 and 16: a. Bordetella pertussis b. Corynebacterium diphtheriae c. Legionella pneumophila d. Mycobacterium tuberculosis B 15. Causes the formation of a membrane across the throat. D 16. Resistant to destruction by phagocytes FULL NAME OF TEACHER-SCHOLAR: LASYAM A. QUISALAN SCHOOL ASSIGNMENT: CANATUAN NATIONAL HIGH SCHOOL PHONE NUMBER: 09673045439 Learning Activity Activity 1: Analyze It! Directions: Analyze and complete the following table below. Use the Rubric “Analyze It” to rate yourself. (70 pts) Here is a completed table for the diseases listed. The information includes the causative agents, vectors, treatments, reservoirs, transmission modes, how to avoid spreading, and how to avoid catching each disease: Disease Causative Agent Vector Treatment Reservoi r Transmissi on 1. Malaria Plasmodium spp. (e.g., P. falciparum) Female Anopheles mosquitoe s Antimalari al drugs (e.g., chloroquin e, artemisinin ) Humans, mosquito es Mosquito bites 2. Yellow Fever Flavivirus Aedes and Haemagog us mosquitoe s Vaccine (prevention ), supportive care Primates, humans Mosquito bites 3. Dengue Dengue virus Aedes mosquitoe s Antipyretic s (fever manageme nt), supportive care Humans, mosquito es Mosquito bites 4. Relapsing Fever Borrelia spp. Lice (epidemic) , soft ticks (endemic) Antibiotics (e.g., doxycyclin e, penicillin) Rodents (endemic ), humans Lice or tick bites, contact with infected person or animals How to How to Avoid the Avoid Spread Catching the Disease Use insect Use repellent, insect sleep repellent, under bed wear nets, use protectiv mosquito e control clothing, avoid mosquito -prone areas Vaccinati Vaccinat on, e, avoid mosquito mosquito repellents, -prone use of areas, nets wear protectiv e clothing Use Wear mosquito protectiv repellents, e eliminate clothing, standing use water, use mosquito nets repellent, avoid standing water Treat lice Avoid infestation lice s, improve exposure, hygiene treat and wash infested clothes, 5. Leishmania sis Leishmania spp. Sandflies Antimicrob ial drugs (e.g., amphoteric in B) Rodents, dogs, humans Sandfly bites Control sandfly populatio ns, avoid exposure during peak hours Isolate infected individual s, wear masks, improve hygiene 6. Viral Pneumonia Various viruses (e.g., influenza virus, RSV) Airborne (droplets) Antiviral drugs (e.g., oseltamivir for influenza), supportive care Humans Airborne (cough, sneeze) 7. Influenza Influenza virus (Types A, B, C) Airborne (droplets) Antiviral drugs (e.g., oseltamivir ), supportive care Humans, pigs, birds Airborne (cough, sneeze) Vaccinati on, cover mouth when coughing, frequent hand washing 8. Tuberculos is Mycobacteri um tuberculosis Airborne (droplets) Antibiotics (e.g., rifampin, isoniazid) Humans Airborne (cough, sneeze) 9. Whooping Cough Bordetella pertussis Airborne (droplets) Antibiotics (e.g., azithromyc in) Humans Airborne (cough, sneeze) Use masks, improve ventilatio n in crowded areas, regular screening Vaccinate (DTaP), isolate infected individual s 10. Scarlet Fever Streptococcu s pyogenes Airborne (droplets) Antibiotics (e.g., penicillin) Humans Airborne (cough, sneeze) Treat infections early, good hygiene practices avoid tick bites Avoid sandfly bites (use repellent, wear protectiv e clothing) Get vaccinate d (e.g., for influenza ), avoid close contact with sick people Get vaccinate d, avoid contact with sick people, wash hands regularly Get tested, avoid prolonge d contact with infected individua ls, Get vaccinate d (DTaP), avoid close contact with infected persons Avoid close contact with infected people.