CELL-DYN® 3000/3500/3700/3200
TROUBLESHOOTING GUIDE
Abbott Diagnostics
A Division of Abbott Laboratories
Abbott Park, IL 60064
ii
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
REVISION STATUS
Revision History
Pages Revised and Added
67483-101 6/2002
First Release
CELL-DYN is a registered trademark of Abbott Laboratories.
This guide was developed and produced by TLO Learning Services in Irving, TX.
Copyright 2002, Abbott Laboratories
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
iii
Notes
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
iv
CELL-DYN® 3000/3500/3700/3200
TROUBLESHOOTING GUIDE
CONTENTS
RBC Diluent Syringe Overpressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
HGB Lyse Empty (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
WBC Lyse Empty (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Lyse Empty (CELL-DYN 3500/3700) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Diluent/Sheath Empty (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Reagent Empty - Diluent, Sheath, HGB Lyse (CELL-DYN 3000) . . . . . . . . . . . . . . . . . . . . . . . . 9
Reagent Empty - Diluent, Sheath, Detergent (CELL-DYN 3500/3700) . . . . . . . . . . . . . . . . . . . 11
Shear Valve Position Fault . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Vacuum Accumulator Wet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Clog Error (RBC or WIC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Flow Error (RBC or WIC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
WOC / WBC Flow Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Elevated PLT Backgrounds / LRI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Controls Out of Range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Controls Out of Range - WBC / WOC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Controls Out of Range - NOC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Controls Out of Range - WIC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
Controls Out of Range - RBC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Controls Out of Range - HGB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Controls Out of Range - MCV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
1
Controls Out of Range - PLT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
WIC / WOC Mismatch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
H & H Mismatch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Incomplete Aspiration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Increased RRBC Flags . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
Background Data Unacceptable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Data Not Transmitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700 . . . . . . . . . . . . . . . . . . . . 55
WOC Flow Cell Cleaning Procedure (CELL-DYN 3000/3500/3700) . . . . . . . . . . . . . . . . . . . . 59
WOC Flow Cell Cleaning Procedure (CELL-DYN 3200) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
2
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
RBC Diluent Syringe Overpressure
RBC DILUENT SYRINGE OVERPRESSURE
RBC Diluent
RBC Diluent
Syringe
Syringe
Overpressure
Overpressure
Check center
section of shear
valve
Is notch in
correct
position?
No
Remove and reinstall
Shear Valve & release
pressure from RBC
Diluent Syringe (See
Note) Run backgrounds
Run
backgrounds
Does error
recur?
Yes
Check RBC
dilution pathway
for pinched/
crimped tubing
No
Problem resolved
Yes
Pinched/
crimped tubing
seen?
Refer to HS or
dispatch service
Yes
Remove crimps/
massage tubing
Go to Diagnostics,
More, Initialization
Run backgrounds
No
Does error
recur?
NOTE:
Remove RBC diluent syringe from
bracket (do not disconnect tubing)
y
y
y
Problem resolved
Yes
Pull down slightly on plunger
Reinitialize analyzer by going
to:
- Main
- Diagnostics
- More
- Initialization
If cannot initialize, cycle power
and reinitialize
Refer to HS or
dispatch service
No
Remove, clean, and
reinstall shear valve &
release pressure from
RBC diluent syringe
(See Note) Run
backgrounds
Does error
recur?
No
Problem resolved
Yes
Yes
Does error
recur?
Clean/replace
RBC Diluent
Syringe
No
Problem resolved
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
3
HGB Lyse Empty (CELL-DYN 3200)
HGB LYSE EMPTY (CELL-DYN 3200)
HGB Lyse Empty
HGB Lyse3200)
Empty
(CELL-DYN
(CELL-DYN 3200)
Press CLEAR
FAULT and
observe HGB Lyse
Syringe
Is syringe
moving
properly?
No
Clean or replace
syringe
Does error
recur?
No
Problem resolved
Yes
Yes
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Is syringe filled
properly? Are
reagent lines not
crimped?
Dispatch Service
No
Disable analyzer,
exercise valve 24
using valve puller
Does error
recur?
Massage and reseat
tubing in valve 28
Yes
Yes
Refer to HS or
dispatch service
Cycle power
Dispatch Service
4
Yes
Does error
recur?
No
Problem resolved
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
WBC Lyse Empty (CELL-DYN 3200)
WBC LYSE EMPTY (CELL-DYN 3200)
WBC Lyse Empty
WBC Lyse3200)
Empty
(CELL-DYN
(CELL-DYN 3200)
Press CLEAR
FAULT and
observe WBC
Lyse Syringe
Is syringe
moving
properly?
No
Clean or replace
syringe
Does error
recur?
Problem resolved
Yes
Yes
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Is syringe filled
properly? Are
reagent lines not
crimped?
No
Dispatch Service
Disable analyzer, and
exercise valve
25 using valve puller
No
Massage and reseat
tubing in valve
(normally closed) 23
Yes
Refer to HS or
dispatch service
Does error
recur?
No
Problem resolved
No
Problem resolved
Yes
Cycle power
Does error
recur?
Yes
Dispatch Service
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
5
Lyse Empty (CELL-DYN 3500/3700)
LYSE EMPTY (CELL-DYN 3500/3700)
L y s e E m p ty
( C E L L - D LYyNs e3 5E0m0 p/3t y7 0 0 )
(C E L L -D Y N 3 5 0 0 /3 7 0 0 )
C h e c k r e a g e n t c u b e a n d in le t lin e s :
- c u b e e m p ty ?
- r e a g e n t c o r r e c t?
- in le t lin e r e a c h e s to b o tto m ?
- v e n t h o le in c a p is o p e n ?
- fo a m s e a l r e m o v e d fr o m u n d e r c a p ?
- lin e s n o t c r im p e d ?
- p o r ts n o t p lu g g e d ?
- c h e c k flu id le v e ls in s y r in g e
P r im e ly s e t o w a s t e c h a m b e r
2 b y r e m o v in g t u b in g f r o m
V 3 6 (N C V )
P re s s C L E A R F A U L T
F lo s s tu b in g in to N C V 3 6
E x e r c is e s o le n o id s
42, 47
D o e s e rro r
re c u r?
Yes
No
D o e s e rro r
re c u r?
No
P r o b le m r e s o lv e d
Yes
C h e c k W IC /H G B L y s e S y r in g e :
- s e a te d a n d m o v in g c o r r e c t ly ?
- tu b in g c r im p e d ?
C le a n /r e s e a t s y r in g e a s n e e d e d
a n d r e m o v e c r im p s
D o e s e rro r
re c u r?
No
P r o b le m r e s o lv e d
Yes
C h e c k ly s e in le t lin e fo r c r im p s
o r p lu g s
R e m o v e ly s e in le t tu b in g & f lu s h
lin e w ith w a rm D I w a te r
P r o b le m r e s o lv e d
No
D o e s e rro r
re c u r?
Yes
No
C y c le P o w e r
D o e s e rro r
re c u r?
Yes
R e fe r to H S o r
d is p a tc h s e r v ic e
6
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Diluent/Sheath Empty (CELL-DYN 3200)
DILUENT/SHEATH EMPTY (CELL-DYN 3200)
Diluent/Sheath Empty
Diluent/Sheath
Empty
(CELL-DYN
3200)
(CELL-DYN 3200)
Press CLEAR FAULT and
open front left door
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Run background
Problem resolved
No
Does error
recur?
Yes
Press CLEAR FAULT, run
background, and observe
diluent and sheath reservoirs
Does fluid reach the
sensor electrodes?
No
Check reservoirs
for cracks / leaks
Yes
Refer to HS or
dispatch service
Cracks / leaks
seen?
No
Check sensor
contacts at top of
reservoirs are
seated properly.
Reseat if
indicated. Run
background count.
Yes
Dispatch service
Does error
recur?
No
Problem resolved
Yes
Page 1 of 2
Go to
A
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
7
Diluent/Sheath Empty (CELL-DYN 3200)
DILUENT/SHEATH EMPTY (CELL-DYN 3200 )
A
Check for salt
buildup at
reservoir
connectors
and clean if
present
Problem resolved
No
Does error
recur?
Yes
- Verify diluent/sheath plumbing is free
of restrictions
- Exercise valves 61, 62, 63, and 64
- Are reservoirs cracked and leaking?
- Clean sensor contacts (electrodes
and connector at top of reservoir) and
contacts are touching
Run background
Problem resolved
No
Does error
recur?
Yes
Cycle power
Problem resolved
No
Does error
recur?
Yes
Refer to HS or
dispatch service
Page 2 of 2
8
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Reagent Empty - Diluent, Sheath, HGB Lyse (CELL-DYN 3000)
REAGENT EMPTY - DILUENT, SHEATH, HGB LYSE (CELL-DYN 3000)
CELL-DYN 3000
CELL-DYN
3000
Reagent
Empty
Reagent
Diluent Empty
Diluent
Sheath
HGBSheath
Lyse
HGB Lyse
Clear fault
Press RUN and
observe reagent
reservoirs
Does fluid
reach sensor
contacts?
No
Yes
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under
cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Clean sensor
contacts
Ensure sensor is
seated correctly
Run background
Does error
recur?
Problem resolved
No
Does error
recur?
No
Problem resolved
Yes
No
Yes
Does error
recur?
Yes
Refer to HS or
dispatch service
Check reservoir for
cracks
Cycle power
Floss NCV tubing
(V6-1 (Diluent), V6-2 (Sheath),
V6-3 (HGB Lyse))
for affected reservoir
or
replace check valve
No
Cracks seen?
Yes
Problem resolved
No
Does error
recur?
Refer to HS or
Dispatch Service
Yes
Page 1 of 2
Go to
A
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
9
Reagent Empty - Diluent, Sheath, HGB Lyse (CELL-DYN 3000)
REAGENT EMPTY - DILUENT, SHEATH, HGB LYSE (CELL-DYN 3000)
A
Exercise vacuum and pressure solenoids for
affected reservoir
Diluent pressure and vacuum - V5-1, 5-2
Sheath pressure and vacuum - V5-3, 5-4
HGB Lyse pressure and vacuum - V5-5, 5-6
Problem resolved
No
Does error
recur?
Yes
Cycle power
Problem resolved
No
Does error
recur?
Yes
Page 2 of 2
10
Refer to HS or
Dispatch Service
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Reagent Empty - Diluent, Sheath, Detergent (CELL-DYN 3500/3700)
REAGENT EMPTY - DILUENT, SHEATH, DETERGENT
(CELL-DYN 3500/3700)
CELL-DYN 3500/3700
CELL-DYN
3500/3700
Reagent Empty
Reagent
Diluent Empty
Diluent
Sheath
Sheath
Detergent
Detergent
Clear fault
Press RUN and
observe reagent
reservoirs
Does fluid reach
sensor contacts?
No
Yes
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Clean sensor
contacts
Ensure sensor is
seated correctly
Does error
recur?
Problem resolved
No
Does error
recur?
No
Problem resolved
Yes
No
Yes
Does error
recur?
Check reservoir for
cracks
Cycle power
Yes
Floss NCV tubing for
affected reservoir
or
replace check valve
Refer to HS or
dispatch service
No
Cracks seen?
Yes
Problem resolved
No
Does error
recur?
Refer to HS or
Dispatch Service
Yes
Page 1 of 2
Go to
A
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
11
Reagent Empty - Diluent, Sheath, Detergent (CELL-DYN 3500/3700)
REAGENT EMPTY - DILUENT, SHEATH, DETERGENT
(CELL-DYN 3500/3700)
A
Exercise vacuum and pressure solenoids for
affected reservoir
V5-1 Diluent pressure
V5-2 Diluent vacuum
V5-3 Sheath pressure
V5-4 Sheath vacuum
V5-5 Detergent pressure
V5-6 Detergent vacuum
Problem resolved
No
Does error
recur?
Yes
Cycle power
Problem resolved
No
Does error
recur?
Yes
Page 2 of 2
12
Refer to HS or
Dispatch Service
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Shear Valve Position Fault
SHEAR VALVE POSITION FAULT
S h e a r V a lv e
h ena rF V
a lt
lv e
P o sSitio
au
P o s itio n F a u lt
D id e rro r o c c u r
a fte r c le a n in g s h e a r
v a lv e ?
C le a n a n d in s p e c t
s h e a r v a lv e
No
Yes
Is s h e a r v a lv e
d a m a g e d /p in s
b ro k e n ?
Yes
R e fe r to H S o r
D is p a tc h s e rv ic e
No
R e m o v e a n d re in s ta ll
s h e a r v a lv e c o rre c tly
(C E L L -D Y N 3 0 0 0 :
u s e lu b ric a n t)
R e in s ta ll s h e a r
v a lv e c o rre c tly
R e in itia liz e s y s te m
D o e s e rro r
re c u r?
No
P ro b le m re s o lv e d
Yes
C y c le p o w e r
D o e s e rro r
re c u r?
No
P ro b le m re s o lv e d
Yes
R e fe r to H S o r
d is p a tc h s e rv ic e
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
13
Vacuum Accumulator Wet
VACUUM ACCUMULATOR WET
Vacuum
Vacuum
Accumulator
W et
Accumulator W et
Observe waste
line for
obstructions or
correct draining
Is waste line
draining
correctly?
No
Remove
obstruction/reseat
tubing
Yes
Drain accumulators
CELL-DYN 3000: Follow Operator's Manual for
manual procedure
CELL-DYN 3500/3700/3200: through
DIAGNOSTICS menu
(go to Diagnostics, More, Drain accumulator)
Reinitialize system
Does error
recur?
No
Problem resolved
No
Problem resolved
Yes
Cycle power
Does error
recur?
Yes
Refer to HS or
dispatch service
14
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Clog Error (RBC or WIC)
CLOG ERROR (RBC OR WIC)
C log E rror (R B C or W IC )
C log E rror (R B C or W IC )
R un background
P roblem resolved
No
D oes error
recur?
P roblem resolved
Y es
C heck reagent cube and inlet lines:
- cube em pty?
- reagent correct?
- inlet line reaches to bottom ?
- vent hole in cap is open?
- foam seal rem oved from under cap?
- lines not crim ped?
- ports not plugged?
- check fluid level in syringe
No
D oes error
recur?
Y es
P roblem resolved
No
R B C C log:
- clean/replace 10 m L syringe
- clean air filter
- run autoclean
D oes error
recur?
V erify aperture plate(s) are correctly
installed and check for cracks
R eseat and clean per O P S m anual
Y es
E xercise solenoids around affected
transducers and m assage and reseat
tubing at solenoids
(S ee N ote)
NOTE:
S olenoids
C E LL-D Y N 3000:
3-1, 3-2, 3-3, 3-6, 3-7, 3-8
D oes error
recur?
C E LL-D Y N 3500/3700:
R B C : 3-1, 3-2, 3-3, 3-4, 3-5, 3-7, 3-8
W IC : 8-1, 8-2, 8-3, 8-4, 8-5, 8-7, 8-8
Y es
No
P roblem resolved
G o to
A
P age 1 of 2
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
15
Clog Error (RBC or WIC)
CLOG ERROR (RBC OR WIC)
A
Check
transducer(s) and
metering tubes for
cracks or leaks
Check quality of
meniscus in
metering tube
No
Cracks/leaks
seen?
Yes
Check fitting between top
of metering tube and V 37 (RBC) or V 8-7 (W IC)
No
Good
meniscus?
Dispatch Service
Clean or flush metering
tube if needed
Yes
Problem resolved
Page 2 of 2
16
No
Does error
recur?
Problem resolved
Yes
No
Cycle power
Does error
recur?
Yes
Refer to HS or
dispatch service
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Flow Error (RBC or WIC)
FLOW ERROR (RBC OR WIC)
Flow Error
Error
(RBCFlow
or WIC)
(RBC or WIC)
Run background
Problem resolved
No
Does error
recur?
Yes
Inspect transducer/vent solenoids and
pinch tubing
Massage and reseat tubing in
solenoids as needed
SEE NOTE
Problem resolved
No
Does error
recur?
Yes
Verify aperture plate(s) are
correctly installed and not
cracked
Reseat/replace if indicated
Problem resolved
No
Does error
recur?
Yes
Check
transducer(s) and
metering tubes for
cracks or leaks
NOTE:
Solenoids
CELL-DYN 3000:
3-1, 3-2, 3-3, 3-6, 3-7, 3-8
CELL-DYN 3500/3700:
RBC: 3-1, 3-2, 3-3, 3-4, 3-5, 3-7, 3-8
WIC: 8-1, 8-2, 8-3, 8-4, 8-5, 8-7, 8-8
Cracks/leaks
seen?
No
Go to
A
Yes
Page 1 of 2
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Dispatch Service
17
Flow Error (RBC or WIC)
FLOW ERROR (RBC OR WIC)
A
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Run background
Problem resolved
No
Does error
recur?
Yes
Note:
To clean RBC or WIC metering tube:
CELL-DYN 3000: V38 (RBC)
CELL-DYN 3500/3200: V38 (RBC), V88 (WIC)
Check quality of
meniscus in
metering tube -- it
should look like an
upside down U.
Check fitting between top of
metering tube and V 3-7
(RBC) or V 8-7 (WIC)and
exercise valves if indicated
Clean metering tubes
(See Note)
No
1. Disconnect tubing above V38 or V88.
2. Connect 20 mL syringe filled with 20% bleach
to tubing.
3. Open V38 or V88 until syringe is half empty.
4. Release solenoid and wait 5 minutes.
5. Open V38 or V88 until syringe is empty and
release solenoid.
6. Fill syringe with HGB Lyse reagent and repeat
step 5.
7. Run background.
Good
meniscus?
Yes
Problem resolved
No
Does error
recur?
Yes
Run autoclean
Does error
recur?
No
Problem resolved
No
Problem resolved
Yes
Cycle power
Refer to HS or
Dispatch Service
Yes
Does error
recur?
Page 2 of 2
18
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
WOC / WBC Flow Errors
WOC / WBC FLOW ERRORS
WOC/WBC Flow
WOC/WBC
Flow
Errors
Errors
Is error occurring
on one sample
only?
Yes
No
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Rerun sample
Does error
recur?
Yes
Problem resolved
No
Does error
recur?
Yes
Verify Results by an
alternate method
Drain/Fill reagents
Does error
recur?
No
Replace reagents
No
Problem resolved
No
Clean or replace
as needed
Yes
Verify syringes:
- seated correctly
- moving correctly
- bubble free
See NOTE
Syringe OK?
Yes
NOTE:
Check the following syringes:
CELL-DYN 3000: WBC metering syringe
CELL-DYN 3200: Sample injection syringe
CELL-DYN 3500/3700: WOC metering sheath syringes
Go to
A
Does error
recur?
No
Problem resolved
Yes
Page 1 of 2
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
19
WOC / WBC Flow Errors
WOC / WBC FLOW ERRORS
A
Check transfer
peristaltic pump tubing
for wear or holes
Replace as needed
SEE NOTE
Does error
recur?
No
Problem resolved
No
Check for pinched
tubing
Exercise bubble
mix solenoid 2-4
Yes
Verify WOC/WBC mixing chamber
fills correctly, bubble mix is good
(Swirl mix for CELL-DYN 3200)
Mixing
chamber OK?
Yes
Dispatch Service
No
Check channel
offsets
(<2.0, N/A on
CELL-DYN 3200)
Run background
Offsets in
specs?
Does error
recur?
No
Problem resolved
No
Yes
Yes
Yes
Cycle power
Clean Shear Valve
Does error
recur?
Yes
Does error
recur?
Cycle power
Yes
Does error
recur?
No
Problem resolved
Dispatch Service
No
Problem resolved
Page 2 of 2
20
NOTE:
CELL-DYN 3000: WBC transfer peripump tubing
CELL-DYN 3200: Sample transfer peripump tubing
CELL-DYN 3500/3700: WOC transfer peripump tubing
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Elevated PLT Backgrounds / LRI
ELEVATED PLT BACKGROUNDS / LRI
Elevated PLT
Elevated/ PLT
backgrounds
LRI
backgrounds / LRI
Check that ground wire is
attached, front covers on Repeat
background
Problem resolved
No
Does problem
recur?
Yes
Run electrical
background
Go to
C
No
No
Is it a CELLDYN 3200?
Yes
Empty/fill flow cell
Is electrical
background =
0?
Does problem
recur?
No
Yes
Yes
Check diluent/sheath
reagent:
- correct list #?
- frozen?
Replace if needed and prime
twice after replacing.
Check diluent/sheath
reagent:
- correct list #?
- frozen?
Replace if needed and prime
twice after replacing
Problem resolved
No
Problem resolved
Does problem
recur?
Yes
Run autoclean
Problem resolved
No
Does problem
recur?
Yes
Go to
A
Page 1 of 4
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
21
Elevated PLT Backgrounds / LRI
ELEVATED PLT BACKGROUNDS / LRI
A
C h e c k D ilu e n t (D ilu e n t/S h e a th
s y rin g e fo r C E L L -D Y N 3 2 0 0 )
s y rin g e fo r le a k s / b u b b le s /b u ild u p
C le a n o r re p la c e if in d ic a te d
R u n b a c k g ro u n d
P ro b le m re s o lv e d
No
D o e s p ro b le m
re c u r?
Yes
C le a n R B C /P L T A p e rtu re P la te
and
(C E L L -D Y N 3 0 0 0 /3 5 0 0 /3 7 0 0 o n ly )
s h e a r v a lv e a s p re c a u tio n
R u n b a c k g ro u n d
P ro b le m re s o lv e d
No
D o e s p ro b le m
re c u r?
Yes
C E L L -D Y N 3 0 0 0 /3 2 0 0 : C h e c k /
re p la c e (if in d ic a te d ) d ilu e n t/
s h e a th filte r
R u n b a c k g ro u n d
P ro b le m re s o lv e d
No
D o e s p ro b le m
re c u r?
Yes
D ra in /fill b a th s a n d re s e rv o irs
(flo w c e ll o n C E L L -D Y N 3 2 0 0 )
P ro b le m re s o lv e d
No
D o e s p ro b le m
re c u r?
Yes
G o to
B
P age 2 of 4
22
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Elevated PLT Backgrounds / LRI
ELEVATED PLT BACKGROUNDS / LRI
B
Perform prepare
for shipping
procedure
Run 3-5
backgrounds
Problem resolved
No
Does problem
recur?
Yes
Clean or drain
vacuum
accumulators
Run background
Problem resolved
No
Does problem
recur?
Yes
Clean fan filters
Run background
NOTE:
Transducer cleaning
Problem resolved
No
Does problem
recur?
Problem resolved
No
Does problem
recur?
Yes
Clean waste line
Run background
1. From Main menu, press:
Special Protocols, Empty Xducers
2. Disconnect vent tubing at top of transducer (at top
right port of first transducer)
3. Connect 20 mL syringe filled with 20% bleach
solution to top of the port
4. Inject bleach solution until the left side of
transducer is half filled and reconnect tubing.
5. Allow bleach to soak for 5 minutes.
6. Press: Fill xducers Main menu Run
7. Run 5 backgrounds
Yes
Check transducer electrodes for
corrosion
Clean transducer if indicated
(to clean transducer, see note:)
Does problem
recur?
Yes
Cycle power
No
Does problem
recur?
No
Problem resolved
Yes
Refer to HS or
Dispatch Service
Page 3 of 4
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
23
Elevated PLT Backgrounds / LRI
ELEVATED PLT BACKGROUNDS / LRI
C
Check for power cords resting on
diluent or waste lines
Remove if found
Run background
Problem resolved
No
Does problem
recur?
Yes
Check waste sensor dummy
plug (if used)
Reseat plug
Problem resolved
No
Does problem
recur?
Yes
Clean fan filters, drain or clean vacuum
accumulators, and waste line
Run background
Problem resolved
No
Does problem
recur?
Yes
Check environment:
- printer/other devices on or near CELL-DYN? (e.g.: centrifuge, air conditioning)
- flickering/fluorescent lights?
- humidity?
- CELL-DYN connected to UPS?
Problem resolved
Remove potential interferences
Problem resolved
No
Does problem
recur?
Yes
No
Does problem
recur?
Cycle power
Yes
Page 4 of 4
24
Refer to HS or
Dispatch Service
Yes
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Controls Out of Range
CONTROLS OUT OF RANGE
Controls Out of
Controls
Range Out of
Range
- Verify QC means/limits
- Check storage/handling
- Condition of QC when
received
- Rerun controls
Does problem
recur?
No
Problem resolved
Check reagent cube and inlet lines:
- cube em pty?
- reagent correct?
- inlet line reaches to bottom ?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Yes
Are backgrounds
within specs?
Troubleshoot
backgrounds
No
Yes
No
Does problem
occur in one mode
only?
Run Fresh
Controls
Yes
Does problem
recur?
No
Problem resolved
Yes
Does problem
recur?
Troubleshoot aspiration
system of affected system
Yes
No
Problem resolved
No
Go to Incomplete
Aspiration Tree
No
Problem resolved
Check sample aspiration
through Shear Valve
Does problem
recur?
No
Yes
Are sam ples
aspirated correctly?
Run precision
Yes
Troubleshooting
im precision
No
Precision within
specs?
Yes
Run autoclean
Yes
Verify mode to mode
calibration
Recalibrate if needed
Does problem
recur?
Yes
Problem resolved
No
Does problem
recur?
Go to OPEN
/ CLOSED
m ode
Page 1 of 10
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
25
Controls Out of Range
CONTROLS OUT OF RANGE
Problem occuring
in OPEN or
CLOSED mode
Issue resolved
OPEN /
CLOSED
Were Controls
rerun?
No
No
Controls still
out of range?
Try a different
box of controls
Yes
Controls still
out of range?
No
Yes
Yes
Issue resolved
Check affected
parameters
Yes
No
Were controls mixed by
hand, warmed to room
temperature for 15
minutes, and stored
inside refrigerator shelf?
No
Are multiple
parameters out of
range?
Redo controls
mixed by hand
No
Yes
Follow flow chart
for affected
parameter
Clean shear valve, clean Y-fitting
(for CELL-DYN 3000/3500/3700)
or Y-valve (for CELL-DYN 3200)
and rerun Controls
Controls within
range?
WOC/
WBC
NOC
WIC
RBC
HGB
MCV
PLT
Go to
A
Go to
B
Go to
C
Go to
D
Go to
E
Go to
F
Go to
G
Yes
Problem resolved
No
Does problem
recur?
Yes
Issue resolved
Yes
Check syringes:
- seated/moving
properly?
- filling?
Clean/replace as
needed
Problem resolved
No
Does problem
recur?
Page 2 of 10
26
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Controls Out of Range - WBC / WOC
CONTROLS OUT OF RANGE - WBC / WOC
A
Run precision
Rerun precision
Verify Sample Injection Syringe
and/or WOC Metering Syringe
are not leaking, have no
bubbles, and are moving up/
down properly
(visually inspect)
No
- change Transfer Pump
Tubing
Is precision
within range?
Precision within
specs?
Yes
Empty/fill WOC
flow cell and rerun
controls
No
Issue resolved
Yes
Yes
Problem resolved
No
Issue resolved
Does problem
recur?
Is placement of
neutrophil population
correct on 0/10
scattergram?
Yes
No
Clean WOC flow
cell or perform
clean for ship and
rerun controls
Controls in
range?
Run normal
patient sample
No
Replace WOC
peripump tubing
and rerun controls
Yes
Check channel offsets
(CELL-DYN 3000/3500/3700 only)
Dispatch service
No
Are channel
offsets < 2.00?
Yes
Verify correct operation of
syringes
(see NOTE)
Clean or replace as needed
NOTE:
Check the following syringes:
CELL-DYN 3000: WBC metering syringe & WOC sheath syringe
CELL-DYN 3200: Sample injection syringe
CELL-DYN 3500/3700: WOC metering syringe & WOC sheath
syringe
Does problem
recur?
No
Problem resolved
Yes
Page 3 of 10
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Go to
AA
27
Controls Out of Range - WBC / WOC
CONTROLS OUT OF RANGE - WBC / WOC
AA
Check WBC/WOC mixing
chamber for proper
draining, filling & bubble
mix
Exercise mix chamber
solenoids
Dispatch service
No
Is mixing chamber
draining/filling
correctly?
Yes
Verify WBC/WOC calibration by performing
a calibration verification and calibrate
if indicated
Run controls
Problem resolved
No
Is the WOC
within ranger?
Yes
Dispatch service
Page 4 of 10
28
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Controls Out of Range - NOC (CELL-DYN 3200 Only)
CONTROLS OUT OF RANGE - NOC (CELL-DYN 3200 ONLY)
B
Run precision
Rerun precision
Verify sample injection syringe
or WOC metering syringe is not
leaking, has no bubbles, and is
moving up/down properly
(visually inspect)
No
- change Transfer Pump
Tubing
Is precision
within range?
Is precision
within specs?
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Yes
Are HGB
results
affected?
No
Yes
Yes
Issue resolved
Clean shear valve
and HGB flow cell
No
Check solenoid 41
Massage and
reseat tubing
Does problem
recur?
No
Problem resolved
No
Problem resolved
Yes
Problem resolved
Problem resolved
Does problem
recur?
No
Yes
Clean/replace as
needed
Check HGB Lyse Syringe:
- seated/moving properly?
- filling?
Clean or replace as
needed
Does problem
recur?
No
Does problem
recur?
Yes
Check staging
pathway from
shear valve to
HGB flow cell for
obstructions
Problem resolved
Check sample
injection syringe
No
Does problem
recur?
Yes
Verify NOC calibration by
performing a calibration
verification and
calibrate if indicated
Rerun controls
Does problem
recur?
No
Problem resolved
Yes
Refer to HS or
dispatch service
Yes
Page 5 of 10
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Refer to HS or
dispatch service
29
Controls Out of Range - WIC
CONTROLS OUT OF RANGE - WIC
C
Refer to HS or
dispatch service
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Run Precision
No
Is precision
within range?
Troubleshoot imprecision
by cleaning WIC aperture
plate
Redo precision
No
Is precision
within specs?
Yes
Rerun
controls
Yes
Problem resolved
Yes
Is WIC within
range?
No
Verify transducer operation by
emptying, filling, draining & bubble mix
Exercise transducer solenoids,
massage and reseat tubing
Refer to HS or
dispatch service
No
See chart of
solenoids
to exercise
Solenoids
8-1, 8-2, 8-3, 8-4,
8-5, and 8-7
Is transducer
operating
correctly?
Yes
Check WIC/HGB Lyse and
Diluent syringes
Clean/replace as needed
Problem resolved
No
Does problem
recur?
Yes
Verify WIC calibration by
performing a calibration
verification
Calibrate if indicated
Rerun controls
Problem resolved
No
Does problem
recur?
Yes
Refer to HS or
dispatch service
Page 6 of 10
30
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Controls Out of Range - RBC
CONTROLS OUT OF RANGE - RBC
D
Run precision
Troubleshoot
Imprecision
Clean shear valve (ensure
inserted correctly)
Replace sample peripump
tubing
(except CELL-DYN 3200)
Is precision
within specs?
No
Yes
Does problem
recur?
Clean RBC aperture plate
(except CELL-DYN 3200)
No
Problem resolved
Yes
Problem resolved
Massage tubing through solenoid
5-4 and verify RBC chamber is
draining, filling, and swirl mixing
properly
for CELL-DYN 3200
Does problem
recur?
No
Yes
Solenoids
3-1, 3-2, 3-3, 3-4,
3-5, and 3-7
Verify transducer operation
Exercise transducer solenoids,
massage and reseat tubing
(except CELL-DYN 3200)
Refer to HS or
dispatch service
Is transducer
operating
correctly?
Does problem
recur?
No
Problem resolved
No
Refer to HS or
dispatch service
No
Problem resolved
Yes
No
CELL-DYN 3200:
check vacuum #2
(3.5" open,
5.0" closed)
Yes
CELL-DYN 3200
Verify RBC mixing chamber
for proper fill/drain; swirl mix
Vac #2 within
specs?
Check Diluent syringe
Clean/replace as needed
Problem resolved
No
Yes
Verify RBC calibration by
performing calibration
verification using calibrator
Calibrate if indicated
Does problem
recur?
Refer to HS or
dispatch service
Yes
Does problem
recur?
Page 7 of 10
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
31
Controls Out of Range - HGB
CONTROLS OUT OF RANGE - HGB
E
Issue resolved
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Run precision
Yes
Troubleshoot HGB
imprecision by
cleaning HGB
Flow Cell and
rerun precision
Is precision
within range?
No
Is precision
within specs?
Yes
Check HGB reference and HGB
voltage within specs and document
No
HGB reference
within specs?
No
Clean HGB flow
cell
Refer to HS or
dispatch service
No
HGB reference
within specs?
Yes
Problem resolved
No
HGB Reference
>2200 for CELL-DYN 3000/
3500/3700
>2050 for CELL-DYN 3200
No
Yes
Refer to HS or
dispatch service
Check HGB syringes
Check:
CELL-DYN 3200 Solenoids 2-4
-- 2-8 and massage tubing
CELL-DYN 3500/3700
Solenoid 3-6
Clean/replace as needed
Yes
Rerun controls
Does problem
recur?
Does problem
recur?
No
Problem resolved
Yes
Verify HGB calibration by performing the
calibration verification
Calibrate if indicated
Refer to HS or
dispatch service
Yes
Does problem
recur?
No
Problem resolved
Page 8 of 10
32
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Controls Out of Range - MCV
CONTROLS OUT OF RANGE - MCV
F
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Run precision
Troubleshoot
Imprecision
No
Is precision
within specs?
Yes
Clean RBC Aperture Plate and RBC / PLT
Transducer
(CELL-DYN 3000/3500/3700 only)
CELL-DYN 3200: verify RBC mixing chamber
is draining and swirl mixing properly
Replace Diluent / Sheath if indicated
Problem resolved
No
Does problem
recur?
Yes
Check RER data
(CELL-DYN 3000/3500/3700 only)
25-35% for normal patient
Refer to HS or
dispatch service
No
Is RER data
within specs?
Yes
Verify MCV calibration by performing
calibration verification
Calibrate if indicated
Rerun controls
Problem resolved
No
Does problem
recur?
Yes
Refer to HS or
dispatch service
Page 9 of 10
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
33
Controls Out of Range - PLT
CONTROLS OUT OF RANGE - PLT
G
Run precision
Troubleshoot imprecision by
cleaning RBC aperture plate
or replacing Diluent / Sheath
filter (for CELL-DYN 3200)
Rerun precision
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Is precision
within specs?
No
CELL-DYN 3200:
RBC / PLT
mix chamber; swirl
Yes
Problem resolved
Is precision
within range?
Yes
No
Refer to HS or
dispatch service
Rerun controls
CELL-DYN 3200:
massage tubing
through solenoid 5-4
Does problem
recur?
Does problem
recur?
Yes
Yes
Verify transducer operation
Exercise transducer solenoids,
massage and reseat tubing
(CELL-DYN 3000/3500/3700
only)
Verify PLT calibration
by performing
calibration verification
No
No
Is transducer
operating
correctly?
No
Problem resolved
No
Problem resolved
Calibrate if indicated
Rerun controls
Yes
Does problem
recur?
Yes
Yes
Check Diluent
syringe
Clean/replace as
needed
Refer to HS or
dispatch service
Issue resolved
Problem resolved
No
Does problem
recur?
Page 10 of 10
34
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)
WIC / WOC MISMATCH (CELL-DYN 3500/3700 ONLY)
W IC / W OC
Mism atch
(m ultiple sam ples)
Run controls in
open m ode
Are W IC and
W OC correct?
No
Yes
Yes
Troubleshoot
closed m ode
Troubleshoot
W OC
No
No
Troubleshoot W IC
Go to
D
Go to
A
Run precision
Troubleshoot
im precision
Is W IC correct?
Precision within
specs?
Yes
Check closed
m ode aspiration
See Incom plete
Aspiration tree
Yes
Problem resolved
No
Does problem
recur?
Yes
Verify m ode to
m ode calibration
Calibrate if
indicated
Problem resolved
No
Does problem
recur?
Page 1 of 6
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
35
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)
WIC / WOC MISMATCH
A
Run precision
Troubleshoot
imprecision
No
Precision within
specs?
Problem resolved
No
Does problem
recur?
Yes
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Yes
Check W OC Sheath and metering syringes:
- filled/seated/ moving properly?
Clean/replace if needed
Problem resolved
No
Does problem
recur?
Yes
Check sample
aspirations/shear
valve
Go to Incomplete
Aspiration tree
No
Is sample
aspirating
correctly?
Yes
Page 2 of 6
36
Go to
B
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)
WIC / WOC MISMATCH
B
Check WOC
mixing chamber
Check solenoids
1-7, 2-3, 2-4
Massage and
reseat tubing
Does chamber
drain/fill and bubble
mix properly?
No
Yes
Problem resolved
No
Does problem
recur?
Problem resolved
Replace WOC
peristaltic pump
tubing
Yes
No
Does problem
recur?
Yes
Check staging pathway from WOC
mixing chamber to flow cell:
Check solenoids 2-1, 2-2, 2-3
Massage and reseat tubing
Problem resolved
No
Does problem
recur?
Yes
Massage and reseat tubing
in solenoids 2-5, 2-7
Problem resolved
No
Does problem
recur?
Go to
C
Page 3 of 6
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
37
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)
WIC / WOC MISMATCH
C
Check channel
offsets
Are channel
offsets less
than 2.00?
No
Dispatch service
or refer to HS
Yes
Verify WOC
calibration
Recalibrate if
indicated
Problem resolved
No
Does problem
recur?
Yes
Page 4 of 6
38
Dispatch Service
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)
WIC / WOC MISMATCH
D
Run Precision
Troubleshoot
imprecision
No
Precision within
specs?
Problem resolved
No
Does problem
recur?
Yes
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Yes
Check WIC/HGB diluent and
lyse syringes:
- filled/seated/moving properly?
Clean/replace if needed
Problem resolved
No
Does problem
recur?
Yes
Clean WIC
Aperture Plate
Problem resolved
No
Does problem
recur?
Yes
Go to
E
Page 5 of 6
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
39
WIC / WOC Mismatch (CELL-DYN 3500/3700 Only)
WIC / WOC MISMATCH
E
Check W IC
transducer
Check solenoids 8-3, 8-4
Massage and reseat tubing
Problem resolved
No
Does problem
recur?
Problem resolved
Is left cham ber of
W IC transducer
draining/filling
properly?
No
Check tubing between top
of transducer and vent
accum ulator for crim ps
Massage and reseat
tubing
Yes
No
Does problem
recur?
Yes
Check sam ple
aspiration/shear
valve
Go to Incom plete
Aspiration tree
No
Is sam ple
aspirating
correctly?
Yes
Verify W IC
calibration
Recalibrate if
indicated
Problem resolved
No
Does problem
recur?
Yes
Page 6 of 6
40
Dispatch Service
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
H & H Mismatch
H & H MISMATCH
H & H Mismatch
H & H Mismatch
Run controls to
determine affected
parameters
Run precision
Is precision
within specs?
No
Troubleshoot
imprecision
Yes
Affected
parameter
MCV
RBC
HGB
Go to
A
Go to
B
Go to
C
Page 1 of 5
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
41
H & H Mismatch
H & H MISMATCH
C heck reagent cube and inlet lines:
- cube em pty?
- reagent correct?
- inlet line reaches to bottom ?
- vent hole in cap is open?
- foam seal rem oved from under cap?
- lines not crim ped?
- ports not plugged?
- check fluid level in syringe
A
P roblem resolved
No
D oes problem
recur?
Y es
R un autoclean
P roblem resolved
No
D oes problem
recur?
Y es
C lean R B C /P LT aperture plate
(C E LL-D Y N 3000/3500/3700 only)
P roblem resolved
No
D oes problem
recur?
Y es
C lean fan filters
P roblem resolved
No
D oes problem
recur?
Y es
P erform C alibration verification
C alibrate if indicated
P roblem resolved
No
D oes problem
recur?
Y es
D ispatch S ervice
P age 2 of 5
42
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
H & H Mismatch
H & H MISMATCH
B
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Problem resolved
No
Does problem
recur?
Yes
Run autoclean
Problem resolved
No
Does problem
recur?
Yes
Check Diluent syringe:
- Moving/filling OK?
- Bubble free?
Clean/replace syringe
Problem resolved
No
Perform
Calibration
verification
Calibrate if
indicated
Does problem
recur?
Yes
Clean RBC/PLT aperture plate
(CELL-DYN 3000/3500/3700 only)
Problem resolved
No
Does problem
recur?
Yes
Does problem
recur?
No
Problem resolved
Yes
Dispatch Service
Page 3 of 5
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
43
H & H Mismatch
H & H MISMATCH
C
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Problem resolved
No
Does problem
recur?
Yes
Run autoclean
Problem resolved
No
Does problem
recur?
Yes
NOTE:
HGB References:
CELL-DYN 3000 1850-2250
CELL-DYN 3500 1850-2250
CELL-DYN 3700 1800-2300
CELL-DYN 3200 1800-2300
Check HGB
Reference
See Note
Clean HGB flow
cell
No
HGB Reference
within specs?
Yes
Refer to HS or
Dispatch Service
No
HGB Reference
within specs?
Check HGB lyse
syringe:
- Moving/filling OK?
- Bubble free?
Yes
Yes
Problem resolved
No
Does problem
recur?
Clean/replace if needed
Does problem
recur?
No
Problem resolved
Yes
Page 4 of 5
44
Go to
D
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
H & H Mismatch
H & H MISMATCH
D
Exercise solenoids:
CELL-DYN 3000/3500/3700: V 1-3
CELL-DYN 3200: V2-4
Massage/reseat tubing
Problem resolved
No
Does problem
recur?
Yes
CELL-DYN 3500/3700:
Exercise WIC transducer drain
solenoids V8-2 and V8-3
Massage and reseat tubing
Problem resolved
No
Does problem
recur?
Yes
Perform calibration
verification
Calibrate if indicated
Problem resolved
No
Does problem
recur?
Yes
Dispatch Service
Page 5 of 5
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
45
Incomplete Aspiration
INCOMPLETE ASPIRATION
Incomplete
Incomplete
Aspiration
Aspiration
(multiple
samples)
(multiple samples)
Does error
occur in both
modes?
Go to
A
No
Yes
Issue resolved
Refer to HS or
Dispatch Service
CELL-DYN 3000/3500/3700
CELL-DYN 3200
Inspect/change
sample peristaltic
pump tubing
Check vacuum #2
Does error
recur?
Vac #2 within
specs?
Yes
Yes
Check sample
sensor
Flush Y-valve
LED on/off
appropriately?
Does error
recur?
No
No
No
Refer to HS or
dispatch service
No
Problem resolved
Yes
Inspect/clean
shear valve and
flush Y-fitting with
10% bleach
Problem resolved
No
Does error
recur?
Yes
Yes
Refer to HS or
Dispatch Service
Page 1 of 2
46
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Incomplete Aspiration
INCOMPLETE ASPIRATION
A
Closed Mode
only
Open Mode
only
Perform Sam ple path cleaning and include:
CELL-DYN 3000/3500/3700 : clean Y-fitting
CELL-DYN 3200: clean Y-valve
Perform Sam ple path cleaning and include:
CELL-DYN 3000/3500/3700 : clean Y-fitting
CELL-DYN 3200: clean Y-valve
Does error
recur?
Does error
recur?
Problem resolved
No
Check and exercise solenoids:
CELL-DYN 3000/3500/3700: 9-5 (open
m ode)
CELL-DYN 3200: Exercise V1-7 and verify
no restrictions between Y-valve and V1-7
Massage/reseat tubing
Check/clean
sam ple/vent
needles
No
Problem resolved
Yes
Yes
Problem resolved
No
Does error
recur?
Does error
recur?
No
Problem resolved
No
Issue resolved
Yes
Yes
Replace aspiration
peripum p tubing
(for CELL-DYN
3000/3500/3700
only)
Check SL LED
Refer to HS or
Dispatch Service
Sensor LED on/off
appropriately?
(except CELL-DYN 3200)
No
Run a Sam ple
Yes
Exercise
CELL-DYN 3000/3500/3700: solenoid 9-6
CELL-DYN 3200: exercise V1-2 and verify no
restrictions between Y-valve and V1-2 m assage/
reseat tubing
Does error
occur?
Yes
Refer to HS or
dispatch service
Run another
Sam ple
Yes
Problem resolved
No
Does error
recur?
Yes
Refer to HS or
Dispatch Service
Does LED sensor to
right of shear valve
turns on/off?
No
Page 2 of 2
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Refer to HS or
dispatch service
Yes
Does error
recur?
No
Issue resolved
47
Increased RRBC Flags
INCREASED RRBC FLAGS
Increased RRBC
Increased
Flags RRBC
Flags
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level in syringe
Problem resolved
No
Does problem
recur?
Yes
Clean Shear Valve
Problem resolved
No
Does problem
recur?
Yes
Replace W OC
peristaltic pump
tubing
Problem resolved
No
Does problem
recur?
Yes
Check W OC sheath and metering syringes
(on CELL-DYN 3200, it is the sample injection
syringe):
- seated and moving properly?
- bubble free?
Clean/replace if needed
Problem resolved
No
Does problem
recur?
Yes
Page 1 of 3
48
Go to
A
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Increased RRBC Flags
INCREASED RRBC FLAGS
A
Check WOC mixing chamber
(on CELL-DYN 3500 and 3700 only) or clean it
if indicated - see WOC Chamber Cleaning Procedure page
60
(on CELL-DYN 3200: check WBC mixing chamber)
For CELL-DYN 3000/3500/3700: Check solenoids
1-7, 2-3, 2-4 and massage and reseat tubing
For CELL-DYN 3200: Exercise solenoids 53, 55, 92
and massage and reseat tubing
Problem resolved
No
Does problem
recur?
No
Does chamber drain/fill and
bubble mix properly?
(on CELL-DYN 3200: does it
swirl mix and drain properly?)
Yes
Check staging pathway from
WOC mixing chamber to flow
cell:
Yes
Massage and reseat tubing in
staging solenoids
Problem resolved
No
Does problem
recur?
Yes
Check channel
offsets
(CELL-DYN 3000/
3500/3700 only)
Are channel
offsets less
than 2.00?
Yes
Check 0°/10°
scattergram
Are lymph/neut
populations
- correctly positioned?
- well defined?
No
Dispatch Service
Yes
Page 2 of 3
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Go to
B
49
Increased RRBC Flags
INCREASED RRBC FLAGS
B
Verify WOC
calibration
Recalibrate if
indicated
Problem resolved
No
Does problem
recur?
Yes
Page 3 of 3
50
Dispatch Service
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Background Data Unacceptable
BACKGROUND DATA UNACCEPTABLE
Background Data
Background Data
Unacceptable
Unacceptable
Check that ground wire is
attached, front covers on.
Repeat background
Problem resolved
Repeat
background
OK?
Yes
No
Run electrical
background
Check for
electrical
interference*
No
No
Is it a CELLDYN 3200?
Is electrical
background
OK?
Yes
Empty/fill flow cell
Does problem
recur?
No
Problem resolved
No
Problem resolved
Yes
Yes
Does problem
recur?
Replace milipore
filter
Clean Aperture
Plates
No
Yes
Problem resolved
No
Does problem
recur/
Does problem
recur?
Yes
Dispatch Service
Problem resolved
Clean Transducers
NOTE: See Elevated PLT
Backgrounds/LRI flow chart for
Transducer cleaning instructions
No
Does problem
recur?
Yes
Yes
*Examples of electrical interference:
- reagent lines near electrical wires
- printer on top of CELL-DYN
- electrical appliances near CELLDYN
- flickering fluorescent lights
Go to
A
Page 1 of 2
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
51
Background Data Unacceptable
BACKGROUND DATA UNACCEPTABLE
A
Check reagent cube and inlet lines:
- cube empty?
- reagent correct?
- inlet line reaches to bottom?
- vent hole in cap is open?
- foam seal removed from under cap?
- lines not crimped?
- ports not plugged?
- check fluid level inNo
syringe
Replace reagent
and prime twice
Clean vacuum accumulator
Clean fan filters
Repeat
background
OK?
Repeat
background
OK?
Yes
Yes
Problem resolved
No
Cycle power
No
Perform prepare
for ship
Problem resolved
Yes
Repeat
background
OK?
Yes
Repeat
background
OK?
No
Page 2 of 2
52
Dispatch Service
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Data Not Transmitting
DATA NOT TRANSMITTING
Data not
Data not
transmitting
transmitting
Start
Has it worked
before?
No
First time to
be hooked-up
to an LIS?
Yes
No
Using current rev
of interface spec?
No
Send current
interface spec
No
Problem resolved
Is set-up
correct?
No
Correct set-up
Does problem
recur?
No
Problem resolved
Yes
Does problem
recur?
Yes
Yes
Check computer set-up
(see NOTE)
Cable connected to proper port
Yes
Yes
Perform serial loop
back test
Go to
A
NOTE:
Refer to Operator's Manual for
computer setup
Page 1 of 2
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
53
Data Not Transmitting
DATA NOT TRANSMITTING
A
Pass?
Failed?
Yes
Yes
Dispatch Service
Cycle power and
reseat LIS cable
Does problem
recur?
No
Problem resolved
Yes
Refer to LIS
vendor
Page 2 of 2
54
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700
RETICULOCYTE TROUBLESHOOTING FOR CELL-DYN 3200, 3500
AND 3700
Reticulocyte
Reticulocyte
Troubleshooting
for
Troubleshooting
CELL-DYN
3200, for
CELL-DYN
3200,
3500
and 3700
3500 and 3700
Verify that stain has been stored in the dark at room
temperature, that Reticulocyte Backgrounds <100 and
exact dilution and incubation instructions have been
followed.
Are calibrated pipettes used?
Go to
A
Both
Rerun QC
Problems with
patients or QC out
of range or both?
Patients Only
QC Only
Is it sample
specific?
No
Go to
A
Yes
Issue resolved
No
Still out of
range?
Rerun
Yes
Is issue
recurring?
Prepare a new dilution
of QC and rerun
No
Issue resolved
Yes
Issue resolved
No
Prepare a new dilution
of same sample and
rerun
Still out of
range?
Yes
Is issue
recurring?
Try new vials of
QC, prepare a new
dilution and rerun
No
Issue resolved
Yes
Issue resolved
No
Redraw sample or verify by
alternate method
Still out of
range?
Yes
Verify pipette is calibrated
and proper technique followed
Yes
Is issue
recurring?
No
Issue resolved
No
Is issue
recurring?
Yes
Go to
A
Issue resolved
Page 1 of 4
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
55
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700
RETICULOCYTE TROUBLESHOOTING FOR CELL-DYN 3200, 3500
AND 3700
A
Is background on
the stain <100?
No
Rerun background
Yes
Check maintenance
- extended autoclean - weekly
- clean metering syringe
- clean sheath syringe
- drain and fill flow cell
Yes
Is background
<100?
No
Maintenance
up to date?
No
Try new stain and
rerun
Redo maintenance
and rerun controls
No
Controls within
range?
Yes
Controls within
range?
No
Do WOC gains and
reticulocyte gains
match?
Yes
Yes
Issue resolved
No
Issue resolved
Yes
1.
2.
3.
Rerun QC
Follow procedure below:
For WOC gains: go to Main menu, Diagnostics, F12,
F1, Set point entry (0, 10, 90), and print screen
For reticulocyte gains: go to Reticulocyte mode,
Diagnostics, F12, F1, WOC (0, 10, 90)
Compare WOC gains and reticulocyte gains (0, 10,
90)
Yes
Do WOC and
reticulocyte gains
match?
No
Go to
B
Go to
C
Page 2 of 4
56
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700
RETICULOCYTE TROUBLESHOOTING FOR CELL-DYN 3200, 3500
AND 3700
B
C
Copy WOC gains as follows:
Enter WOC values (0, 10, 90) into
reticulocyte gains screen and
press Set analyzer to save entry
Rerun patient
samples
Yes
QC within
range?
Rerun QC
No
Issue resolved
Yes
Patient
samples OK?
Run 3 -- 5 normal patient
samples in regular patient
mode
No
Controls within
range?
No
Verify by alternate
method
Yes
Issue resolved
Is neutrophil population
at 0/10 scattergram at
the 2.5 by 2.5 hash
marks?
Yes
Rerun QC
No
Controls within
range?
No
Dispatch Service
Check channel offsets are
<2.0
(only for CELL-DYN 3500
and 3700)
Yes
Issue resolved
Go to
D
Page 3 of 4
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
57
Reticulocyte Troubleshooting for CELL-DYN 3200, 3500 and 3700
RETICULOCYTE TROUBLESHOOTING FOR CELL-DYN 3200, 3500
AND 3700
D
Issue resolved
Yes
Controls within
range?
Rerun controls
Yes
Channel offsets within
range?
(only for CELL-DYN 3500
and 3700)
No
Drain/fill flow cell, run autoclean, clean
WOC mixing chamber
(see WOC mixing chamber cleaning
procedure) and recheck channel
offsets
(only for CELL-DYN 3500 and 3700)
No
Issue resolved
Yes
Controls within
range?
Rerun QC
No
Dispatch Service
References:
1. CELL-DYN 3500 Operator's Manual, June 1999: Chapter 14, 14-1 to 14-8
2. CELL-DYN 3700 Operator's Manual, November 2000: Chapter 14, 14-1 to 14-87
3. CELL-DYN 3200 Operator's Manual, October 2001: Chapter 14
Page 4 of 4
58
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
WOC Flow Cell Cleaning Procedure (CELL-DYN 3000/3500/3700)
WOC FLOW CELL CLEANING PROCEDURE
(CELL-DYN 3000/3500/3700)
Materials/Tools Needed:
•
25% Bleach solution
•
10 mL syringe
Step
Action
1
Fill syringe with 25% bleach solution.
2
From the Main Menu, press
•
•
3
SPECIAL PROTOCOLS
DISABLE/ENABLE ANALYZER
Insert bleach into WBC/WOC mixing chamber.
NOTE: Follow the steps below to avoid damaging the plastic port of
mixing chamber on CELL-DYN 3500/3700.
a. Follow tubing from mixing chamber to Overflow Chamber.
CELL-DYN 3000: top port of WBC mixing chamber
CELL-DYN 3500/3700: left top port of WOC mixing chamber
b. Disconnect tubing from overflow chamber.
c. Connect syringe to tubing.
d. Inject bleach solution and fill WBC/WOC mixing chamber 2/3 full.
e. Pull back on syringe slightly to remove any excess pressure and liquid
trapped in tubing.
f.
4
Remove syringe and reconnect tubing to overflow chamber.
For Special Protocols Menu, press
•
ENABLE ANALYZER
5
Perform 2 autocleans with water to allow bleach to enter and clean flow cell.
6
Run 3 background counts.
7
Verify background counts are within specifications.
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101
59
WOC Flow Cell Cleaning Procedure (CELL-DYN 3200)
WOC FLOW CELL CLEANING PROCEDURE
(CELL-DYN 3200)
Materials/Tools Needed:
•
25% Bleach solution
•
10 mL syringe with tubing attached
Step
Action
1
Fill syringe with 25% bleach solution.
2
From the Main Menu, press
•
•
3
SPECIAL PROTOCOLS
DISABLE/ENABLE ANALYZER
Insert bleach into WBC/WOC mixing chamber.
a. Remove tubing from mixing chamber (WBC or RBC/PLT).
b. Connect syringe and attach tubing to mixing chamber.
c. Inject bleach solution and fill WBC mixing chamber 2/3 full.
d. Pull back on syringe slightly to remove any excess pressure and liquid
trapped in tubing.
e. Remove syringe and reconnect tubing to overflow chamber.
4
For Special Protocols Menu, press
•
60
ENABLE ANALYZER
5
Perform 2 autocleans with water to allow bleach to enter and clean flow cell.
6
Run 3 background counts.
7
Verify background counts are within specifications.
CELL-DYN® 3000/3500/3700/3200 Troubleshooting Guide
67483-101