Uploaded by Kimbrea Dejae

Fermentation Lab Exercise: Microbiology Tests & Analysis

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-
Growth of microbe produces gases and acids
The determines ph
Not enough time can give
a false positive.
-
(A)
4
=
S
(B) 5
To define fermentation and how it is a useful tool in examining microorganisms.
00
y
yellow
=
rec
4
-
+
-
+
y
+
-
+
+
red-t
-
+y
BS
-
-
r
-
+
r
+
-
+
+
+
+
y
+
+
-
-
PA
+red
SAT +y
-
+
r
+
-
-
+
-
I
y
+
Furbity bubble
-
gas
+
+
y
+
t
+
S drops ofMR reagent
I
-
PA
-
BS
SA
-
xell
-
SA
BS
I
wii
VP
-y
Is
+
-
-
rea+y-rateabrown
red
I
green
-
PA
I
Redrangee
red
I
-
brown--
+
blue
I
blue
green
-
red
+
+
G
no
chang:-
-
I
2
+
-
Yes
No
MRVP test
It allows time for fermentation
It will become basic
and produce peptone ammonia.
Peptones
Orange/Yellow
Citrate Agar
Only bacteria that can use
citrate as their source and NH4 as
their nitrogen source can grow.
Yes,some fermenters can produce excess neutral products other than acetone; alcohol.
Color change and form gases
If it cannot breakdown the simplest form of sugar (carbohydrates) then it won’t be able to breakdown
a more complex molecule.
Tube will stay the same but gas may occur.
It can grow peptones and media will be turbid.
The drink has been fermented by microorganisms and gas was released.
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