- Growth of microbe produces gases and acids The determines ph Not enough time can give a false positive. - (A) 4 = S (B) 5 To define fermentation and how it is a useful tool in examining microorganisms. 00 y yellow = rec 4 - + - + y + - + + red-t - +y BS - - r - + r + - + + + + y + + - - PA +red SAT +y - + r + - - + - I y + Furbity bubble - gas + + y + t + S drops ofMR reagent I - PA - BS SA - xell - SA BS I wii VP -y Is + - - rea+y-rateabrown red I green - PA I Redrangee red I - brown-- + blue I blue green - red + + G no chang:- - I 2 + - Yes No MRVP test It allows time for fermentation It will become basic and produce peptone ammonia. Peptones Orange/Yellow Citrate Agar Only bacteria that can use citrate as their source and NH4 as their nitrogen source can grow. Yes,some fermenters can produce excess neutral products other than acetone; alcohol. Color change and form gases If it cannot breakdown the simplest form of sugar (carbohydrates) then it won’t be able to breakdown a more complex molecule. Tube will stay the same but gas may occur. It can grow peptones and media will be turbid. The drink has been fermented by microorganisms and gas was released.