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Histology Staining Techniques Study Guide

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What is the combination of histology and biochemistryHistochemical Techniques
Histochemical TechniquesWhat is the combination of histology and biochemistry
What factor facoring or hindering dye-tissue/reagent-tissue arises from electro static attraction?Reagent - Tissue Interaction
Reagent - Tissue InteractionWhat factor facoring or hindering dye-tissue/reagent-tissue arises from electro static attraction?
What factor favoring or hindering dye-tissue/reagent-tissue results from hydrophobic event of some solvent?Solvent - Solvent Interaction
Solvent - Solvent InteractionWhat factor favoring or hindering dye-tissue/reagent-tissue results from hydrophobic event of some solvent?
What factor favoring or hindering dye-tissue/reagent-tissue is due to dye molecules that tend to attract each other - forming aggregates?Stain - Stain Interaction
Stain - Stain InteractionWhat factor favoring or hindering dye-tissue/reagent-tissue is due to dye molecules that tend to attract each other - forming aggregates?
What factor favoring or hindering dye-tissue/reagent-tissue may be filled with multiple or no stain?Some Unusual Possibilities
Some Unusual PossibilitiesWhat factor favoring or hindering dye-tissue/reagent-tissue may be filled with multiple or no stain?
What stain is the most commonly used dyeHematoxylin & Eosin
Hematoxylin & EosinWhat stain is the most commonly used dye
True or False: Hematoxylin & Eason is affected by the thickness of the sectionTrue
TrueTrue or False: Hematoxylin & Eason is affected by the thickness of the section
What stain confirms basic tissue types & helps to localize lesion?Hematoxylin & Eosin
Hematoxylin & EosinWhat stain confirms basic tissue types & helps to localize lesion?
What stain binds strongly to acids (nuclear DNA) and stain the nuclei blueblack/blue? Hematoxylin
HematoxylinWhat stain binds strongly to acids (nuclear DNA) and stain the nuclei blueblack/blue? 
What stains the cytoplasm & tissue fibers pink/orange/red?Eosin
EosinWhat stains the cytoplasm & tissue fibers pink/orange/red?
What stain consists of methylene-blue & eosin and are mostly used for blood films/blood smears?Giemsa Stain
Giemsa StainWhat stain consists of methylene-blue & eosin and are mostly used for blood films/blood smears?
What is the causative agent for syphilis?Spirochetes or Treponema Pallidum
Spirochetes or Treponema PallidumWhat is the causative agent for syphilis?
What is the causative agent for malaria?Plasmodium
PlasmodiumWhat is the causative agent for malaria?
What stain is used to identify & differentiate bacteria?Gram Stain
Gram StainWhat stain is used to identify & differentiate bacteria?
What is the color for negative gram stain?Pink
PinkWhat is the color for negative gram stain?
What is the coloro of positive gram stain?Violet
VioletWhat is the coloro of positive gram stain?
"What stain is used to identify ""acid fast bacilli"" and appears as purply rods?"Ziehl Nielsen Stain
Ziehl Nielsen Stain"What stain is used to identify ""acid fast bacilli"" and appears as purply rods?"
What is an example of the acid fast bacilli that causes tuberculosis & leprosy?Myobacteria
MyobacteriaWhat is an example of the acid fast bacilli that causes tuberculosis & leprosy?
What stains the myelin as blue and other elements of the nervous system as pink/violet?Luxol Fast Blue/Cresyl Violet
Luxol Fast Blue/Cresyl VioletWhat stains the myelin as blue and other elements of the nervous system as pink/violet?
"<span style=""background-color: rgb(255, 255, 255);"">What stain is used for fats (neutral lipids) and is signifant for diagnosing multiple sclerosis?</span>"Oil Red O (ORO)
Oil Red O (ORO)"<span style=""background-color: rgb(255, 255, 255);"">What stain is used for fats (neutral lipids) and is signifant for diagnosing multiple sclerosis?</span>"
"<span style=""background-color: rgb(255, 255, 255);"">What benefits from Bouin's Fixative?</span>"Masson's Trichrome Stain
Masson's Trichrome Stain"<span style=""background-color: rgb(255, 255, 255);"">What benefits from Bouin's Fixative?</span>"
"<span style=""background-color: rgb(255, 255, 255);"">What are the colors their respective site of action under Masson's Trichrome Stain?</span>"<ul><li>cell cytoplasm & muscle keratin - red</li><li>nuclei - black</li><li>collagen - blue</li></ul>
<ul><li>cell cytoplasm & muscle keratin - red</li><li>nuclei - black</li><li>collagen - blue</li></ul>"<span style=""background-color: rgb(255, 255, 255);"">What are the colors their respective site of action under Masson's Trichrome Stain?</span>"
"<span style=""background-color: rgb(255, 255, 255);"">What stains the carbohydrates as magenta and are combined with alcian blue?</span>"Periodic Acid Schiff (PAS)
Periodic Acid Schiff (PAS)"<span style=""background-color: rgb(255, 255, 255);"">What stains the carbohydrates as magenta and are combined with alcian blue?</span>"
"<span style=""background-color: rgb(255, 255, 255);"">What stain is used for fungi and parasites?</span>"Periodic Acid Schiff (PAS)
Periodic Acid Schiff (PAS)"<span style=""background-color: rgb(255, 255, 255);"">What stain is used for fungi and parasites?</span>"
"<span style=""background-color: rgb(255, 255, 255);"">What stains the deposits of protein in tissues?</span>"Congo Red
Congo Red"<span style=""background-color: rgb(255, 255, 255);"">What stains the deposits of protein in tissues?</span>"
"<span style=""background-color: rgb(255, 255, 255);"">What do you call the deposits of tissue that are stained by Congo Red?</span>"Amyloid
Amyloid"<span style=""background-color: rgb(255, 255, 255);"">What do you call the deposits of tissue that are stained by Congo Red?</span>"
"<span style=""background-color: rgb(255, 255, 255);"">What stains the deposits of silver as black?</span>"Silver Staining Method
Silver Staining Method"<span style=""background-color: rgb(255, 255, 255);"">What stains the deposits of silver as black?</span>"
"<span style=""background-color: rgb(255, 255, 255);"">What stain is useful for identifying cells within a group of cell?</span>"Silver Staining Method
Silver Staining Method"<span style=""background-color: rgb(255, 255, 255);"">What stain is useful for identifying cells within a group of cell?</span>"
"<span style=""background-color: rgb(255, 255, 255);"">What stain is a versatile dye, stains the nuclei as blue, and is commonly used as a preliminary stain?</span>"Toluidine Blue
Toluidine Blue"<span style=""background-color: rgb(255, 255, 255);"">What stain is a versatile dye, stains the nuclei as blue, and is commonly used as a preliminary stain?</span>"
"<span style=""background-color: rgb(255, 255, 255);"">What stain binds to collagen in the ECM </span>"Van Gieson Stain
Van Gieson Stain"<span style=""background-color: rgb(255, 255, 255);"">What stain binds to collagen in the ECM </span>"
"<span style=""background-color: rgb(255, 255, 255);"">What are the seven steps in tissue processing? (<span style=""color: rgb(170, 0, 0);"">Clue: FDCIESS</span>) </span>"<ol><li><b>FIxation </b>- to preseve cellular structures</li><li><b>Dehydration </b>- removal of excess water</li><li><b>Clearing</b> - removing dehydrating agents</li><li><b>Infiltration</b> - permeating the tissue with a support media</li><li><b>Embedding</b> - paraffin wax is utilized</li><li><b>Sectioning/Cutting</b> </li><li><b>Staining</b> </li></ol>
<ol><li><b>FIxation </b>- to preseve cellular structures</li><li><b>Dehydration </b>- removal of excess water</li><li><b>Clearing</b> - removing dehydrating agents</li><li><b>Infiltration</b> - permeating the tissue with a support media</li><li><b>Embedding</b> - paraffin wax is utilized</li><li><b>Sectioning/Cutting</b> </li><li><b>Staining</b> </li></ol>"<span style=""background-color: rgb(255, 255, 255);"">What are the seven steps in tissue processing? (<span style=""color: rgb(170, 0, 0);"">Clue: FDCIESS</span>) </span>"
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