INTRODUCTION
CD-TOC
• Section Table of Contents
• 1 General Information
• 2 Theory of Operation
• 3 System Description
• 4 Circuit Description
• 5 Diagnostics and
Troubleshooting
• 6 Diagrams and Schematics
• 7 Removal and Replacement
• 8 Alignment and Verification
Procedures
• Appendix A Planned
Maintenance
• Appendix B Closed Sample
Option
• Appendix C CELL-DYN 1700
System Interface
Specification
Abbott Laboratories
Abbott Park, IL 60064
9140265A - February 1995
EXIT
CELL-DYN 1700 SYSTEM
SERVICE MANUAL
Part Number 9211417
©1995, Abbott Diagnostics
Abbott Diagnostics is a wholly owned
subsidiary of Abbott Laboratories
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INTRODUCTION
Table of Contents
• Proprietary Information
• Revision Status
CELL-DYN® 1700 Service Manual
9140265A-February 1995
1
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INTRODUCTION
Proprietary Information
Abbott Laboratories software programs are protected by copyright. All rights reserved.
No part off this document may be reproduced, stored, retrieved, or transmitted in any form or by any
means without the prior written permission of Abbott Laboratories.
This service manual was developed for use in the field by trained Abbott Laboratories Field Service
Representatives. The revision status of the manual is the responsibility of the manual holder.
In no event shall Abbott Laboratories or its subsidiaries be liable for any damages incurred in connection with or arising from the use of this manual by persons not fully trained by Abbott Laboratories.
The examples contained in this manual are intended for illustrative purposes only.
CELL-DYN® is a registered trademark of Sequoia-Turner Corporation, a wholly owned subsidiary of
Abbott Laboratories.
QAPlus© is a registered trademark of DiagSoft, Inc.
TYGON® is a registered trademark of Norton Performance Plastics.
VACUTAINER® is a registered trademark of Becton, Dickinson, and Company.
© 1995 by Abbott Laboratories. All rights reserved.
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INTRODUCTION
Revision Status
The revision status of the CELL-DYN® 1700 Service Manual is indicated below. It is the responsibility
of the Field Service Representative to verify that the manual contains the latest revision number of all
pages. Additional copies of this manual may be purchased.
Revision number:
Pages revised and/or added:
Originally issued February, 1995
Not applicable.
Table 1:
Document
Control
Number
Revision Record
Software
Revision
Date
Version
Incorporated
Incorporated
(If Applicable) by (Name)
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Document
Control
Number
INTRODUCTION
Software
Revision
Date
Version
Incorporated
Incorporated
(If Applicable) by (Name)
Record document control number and sign and date this log to
provide a permanent record of revisions.
CELL-DYN® 1700 Service Manual
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Section 1
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Section 1.
GENERAL INFORMATION
Table of Contents
1.1 SECTION OVERVIEW
1.2 PURPOSE AND SCOPE
1.3 SERVICE EXPERIENCE
1.4 OPERATING INSTRUCTIONS
1.5 HOW TO USE THIS MANUAL
Service Manual Organization
Accident Prevention Labels
Biohazard Safety Precautions
Biohazard Safety
Sharps
Biohazard Disposal
Biohazard Spills
Manual Revision Information
1.6 SYSTEM SPECIFICATIONS
Physical Specifications
Operational Specifications
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Section 1
GENERAL INFORMATION
Aspiration Volumes — CELL-DYN 1700 and 1700CS
Collection Tube and Sample Volume
Measurement Specifications — CELL-DYN 1700 and 1700CS
Performance Specifications
Background Counts
Linearity
Carryover
Within Sample Precision
Accuracy
Bias
Performance Characteristics
Typical Precision
List of Tables
Table 1-1 Physical Specifications (Dimensions) — CELL-DYN 1700
Table 1-2 Dimensions After Packaging for Shipment — CELL-DYN 1700
Table 1-3 Physical Specifications — CELL-DYN 1700CS
Table 1-4 Dimensions After Packaging for Shipment — CELL-DYN 1700CS
Table 1-5 Power Specifications — CELL-DYN 1700 and 1700CS
Table 1-6 Printer Input Requirements
Table 1-7 Power Consumption — CELL-DYN 1700 and 1700CS
Table 1-8 Operating Environment — CELL-DYN 1700 and 1700CS
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Section 1
GENERAL INFORMATION
Table 1-9 Complete Cycle Times — CELL-DYN 1700 and 1700CSTable 1-10 Aspiration Volumes
Table 1-11 WBC and Differential
Table 1-12 RBCs and PLTs
Table 1-13 HGB
Table 1-14 Background Counts
Table 1-15 Linearity Specifications
Table 1-16 Carryover — Open, Pre-Dilute, and Closed Modes
Table 1-17 Within Sample Precision of the Hemogram Parameters — Open Mode
Table 1-18 Within Sample Precision of the Hemogram Parameters — Closed Mode
Table 1-19 Precision of WBC Differential Parameters — Open and Closed Modes
Table 1-20 Whole Blood Accuracy Results — Open and Closed Modes
Table 1-21 Typical Within Sample Precision Results — Open Mode
Table 1-22 Typical Within Sample Precision Results — Closed Mode
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Section 1
1.1 SECTION OVERVIEW
GENERAL INFORMATION
This section provides a list of the major components and sub-assemblies of the CELL-DYN 1700, a
brief description of the remaining sections in this manual, a discussion of how to use this manual, and
an overview of the instrument’s physical, operational, and measurement specifications.
1.2 PURPOSE AND SCOPE
This manual contains service information for the CELL-DYN 1700 Automated Hematology Analyzer.
Included in this manual are the theory of operation, system and circuit descriptions, alignment and
verification procedures, diagrams and schematics, and board-level replacement procedures for all
major system components.
The CELL-DYN 1700 Automated Hematology Analyzer is a complex system. Analyzer performance
depends on several external components that together make up the complete hematology system.
Each system comprises the following components and subsystems:
•
•
•
•
•
OPERATOR/OPERATOR TECHNIQUE (MAINTENANCE)
REAGENT SYSTEM
— DILUENT
— DETERGENT
— LYSE
PATIENT AND CONTROL SAMPLES
ENVIRONMENT/POWER LINE INTEGRITY
CELL-DYN 1700 ANALYZER
— SYRINGE ASSEMBLY
— FLUID POWER SUPPLY
— CLOSED SAMPLE ASSEMBLY
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Section 1
— FLOW PANEL SYSTEM
— MEASUREMENT ELECTRONICS
— USER INTERFACE COMPUTER
GENERAL INFORMATION
1.3 SERVICE EXPERIENCE
Based on experience and service history, the incidence of hematology problems and their causes
tend to occur in the same descending order of components and subsystems listed above. Note that
the majority of problems and their causes will originate with components external to the analyzer. It
follows that all external components and conditions, such as reagents, environment, integrity of samples and controls, etc., be checked and verified as correct before performing service on the analyzer.
In the investigation of any complaint, the instrument should be the last component of the system to be
suspected.
1.4 OPERATING INSTRUCTIONS
It is essential that the Field Service Representative read and understand the CELL-DYN 1700 Operations Manual, and be able to correctly perform all routine operating functions before attempting to
troubleshoot and repair the system.
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Section 1
1.5 HOW TO USE THIS MANUAL
GENERAL INFORMATION
Service Manual Organization
This service manual is organized into the following sections to facilitate its use in the operation, troubleshooting, repair, and alignment and verification of the CELL-DYN 1700:
Section 1
Section 2
Section 3
Section 4
Section 5
Section 6
Section 7
Section 8
Section 9
General Information
Theory of Operation
System Description
Circuit Descriptions
Diagnostics and Troubleshooting
Diagrams and Schematics
Removal and Replacement
Alignment and Verification Procedures
Appendices
The General Information section gives an overview of the service manual, discusses how to use the
manual, and provides the physical and operational specifications of the CELL-DYN 1700 system.
The Theory of Operation section describes the electrical impedance principle and its application to an
electronic particle counter in the measurement of RBC, WBC and PLTs. This section also describes
the sample flow sequence in the Open, Pre-Dilute, and Closed modes and discusses the methods
used to accurately size the cells and generate histograms for the measurement of MCV, MPV, RDW,
and PDW.
The System Description section provides a system overview as well as a brief description of the major
subsystems.
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Section 1
GENERAL INFORMATION
The Circuit Descriptions section describes the individual circuits in the analyzer and provides simplified schematics of these circuits to aid in the understanding of their function.
The Diagnostics and Troubleshooting section provides a guide for the classification of potential analyzer fault conditions and to aid in their isolation and correction. This section also includes a list of
system status error codes and their explanations and a list of service commands to initiate operations
of individual devices and/or systems.
The Diagrams and Schematics section includes the assembly diagrams and flow system schematics
necessary to troubleshoot and repair the CELL-DYN 1700.
The Removal and Replacement section includes detailed system disassembly and board-level
replacement information.
The Alignment and Verification Procedures section provides step by step instructions for correct electronic alignment and verification of the CELL-DYN 1700 analyzer to ensure optimum performance of
the analyzer. These procedures also function as a diagnostic tool to isolate a defective module or
Printed Circuit Board.
Appendices provide additional documentation regarding planned maintenance, RS-232 Interface
Specifications, and the Closed Sample flow sequence.
Accident Prevention Labels
Danger, Warning, Caution, and Note labels are provided as needed in this service manual to warn,
inform, and assist the Field Service Representative. Each label, and the information associated with
it, is enclosed in a border for easy visual identification. The labels denote information as follows:
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GENERAL INFORMATION
DANGER
Denotes possible death or serious injury; failure to comply will
expose the operator or Field Service Representative to significant
risk of serious injury or death.
WARNING
Denotes clear and present danger or questionable result efficacy;
failure to comply may result in incorrect instrument performance
leading to instrument failure, generation of erroneous results, or
hazard to the operator or Field Service Representative.
CAUTION
Denotes minor, non-immediate, or potential hazard; failure to
comply may result in unexpected instrument performance or may
expose the operator or Field Service Representative to potentially
hazardous conditions.
NOTE:
Denotes general information and helpful hints; failure to comply will present no safety, efficacy, or performance issues.
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Section 1
GENERAL INFORMATION
The following warning icons are displayed where appropriate in this manual.
WARNING: Potential Biohazard. The biohazard icon
alerts users to an activity or area where they may be
exposed to infectious materials or substances.
WARNING: Electrical Shock Hazard. The electrical
warning icon alerts users to the possibility of electrical
shock in the described activity or at the posted location.
Static. Failure to comply may cause damage to the
Printed Circuit Board. Observe precautions for handling
Electrostatic Sensitive Devices (ESD).
CAUTION. The general caution icon appears adjacent
to an explanation of conditions that could interfere with
the proper functioning of the instrument.
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Section 1
Biohazard Safety Precautions
GENERAL INFORMATION
When working on the CELL-DYN 1700, service personnel should keep the following hazards in mind.
Biohazard Safety
Consider all clinical specimens, reagents, controls and calibrators, etc. that contain human blood or
serum as potentially infectious. Wear gloves, lab coats, and safety glasses, and follow other biosafety
practices.
Sharps
The Sample Probe, electrodes, and Cap Piercing Needle (CELL-DYN 1700CS) are sharp and may be
contaminated with potentially infectious materials. Avoid contacting them before they have been
decontaminated.
Biohazard Disposal
All clinical specimens, reagents, controls, calibrators, cuvettes, and other disposables that may be
contaminated must be disposed according to local, state, and federal regulations governing the treatment of regulated medical waste. The probe must be placed in an appropriately marked punctureresistant container prior to disposal.
Biohazard Spills
Clean spills of potentially infectious materials in accordance with established biosafety practices.
Absorb spill with absorbent material, wipe area with detergent solution, then wipe area with disinfectant (10% solution of a 5% bleach).
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Section 1
Manual Revision Information
GENERAL INFORMATION
Revision pages keep the CELL-DYN 1700 Service Manual up to date with configuration changes and
servicing techniques. A new title page with the revision log will be sent with each change package.
This list will contain the page number of each changed or added page along with the revision dash (-)
number of that page. Pages not listed are original pages.
If either the information or the spare part is unique to United States Field Service only, then US is
noted beside the item. If the item is unique to International Field Service only, then INTL is noted
beside the item.
The two following symbols are used to show areas or sections in this service manual which have been
affected by a TSB or an ISA. For example:
T 25
TSB 60-025
Installed
T 25
TSB 60-025 Not
Installed
The following symbol is used to identify the ISA containing additional information about the part or
area.
I 25
“NOTES” Page
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Section 1
GENERAL INFORMATION
The reverse side of each section’s table of contents is reserved for the Field Service Representative
to insert helpful hints, comments regarding errors, or suggestions for improvement.
1.6 SYSTEM SPECIFICATIONS
Physical Specifications
Physical specifications for the CELL-DYN 1700 and CELL-DYN 1700CS are listed in Tables 1-1
through 1-7.
Analyzer
Printer
Height
Width
Depth
Weight
19" (49 cm)
34" (87 cm)
24" (61 cm)
145 lb (66 kg)
5" (13 cm)
16 " (41 cm)
14" (36 cm)
16 lb (7 kg)
Table 1-1:
Physical Specifications (Dimensions) — CELL-DYN 1700
Analyzer
Printer
Height
Width
Depth
Weight
30" (76 cm)
42" (107 cm)
32" (81 cm)
200 lb (91 kg)
9" (23 cm)
22" (56 cm)
20" (51 cm)
35 lb (16 kg)
Table 1-2:
Dimensions After Packaging for Shipment — CELL-DYN 1700
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GENERAL INFORMATION
Analyzer
Height
Width
Depth
19” (49 cm)
34" (87 cm)
26" (66 cm)
Weight
155 lb (71 kg)
Table 1-3:
Physical Specifications (Dimensions) — CELL-DYN 1700CS
Analyzer
Height
Width
Depth
Weight
Table 1-4:
Setting
100
120
220
240
Table 1-5:
30" (76 cm)
42" (107 cm)
32" (81 cm)
210 lb (96 kg)
Dimensions After Packaging for Shipment —CELL-DYN 1700CS
Range
90—110 VAC
110—130 VAC
200—240 VAC
220—260 VAC
Frequency
50/60 Hz
50/60 Hz
50/60 Hz
50/60 Hz
Power Spec. (Analyzer Input Requirements) — CELL-DYN 1700 and 1700CS
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Section 1
Setting
Frequency
120
50/60 Hz
Table 1-6:
Analyzer
Printer
Connector
Table 1-7:
GENERAL INFORMATION
Printer Input Requirements
Avg. 410 Watts (1460 BTU per hour)
Max. 600 Watts (2130 BTU per hour)
120 Watts
Three-prong grounded outlet (U.S.)
Power Consumption — CELL-DYN 1700 and 1700CS
Operational Specifications
Operational specifications for the CELL-DYN 1700 and CELL-DYN 1700CS are listed in Tables 1-8
and 1-9.
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Section 1
Temperature
Instrument
GENERAL INFORMATION
Patient Samples - Room Temperature 23°C
(73°F)
Monocyte values exhibit a change at lower and
higher temperatures. A 1.5% decrease is
seen in the total monocyte percent at lower
temperatures <18°C (64°F). A 6% increase will
be seen at higher temperatures.
15°C to 30°C (59°F ( 86°F)
Relative
Humidity
10% to 85%, noncondensing
Location
Flat, level surface, no direct sunlight or drafts.
Remove from sources of direct heat or moisture.
Ventilation space at least 6" on top, sides, and
back. Do not place next to a heat generating
device. Do not block fans.
Table 1-8:
Operating Environment — CELL-DYN 1700 and 1700CS
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Section 1
GENERAL INFORMATION
Auto-Startup (from STANDBY) Approximately 250 seconds
Auto-Startup (from power OFF) Approximately 300 seconds
Run, Open mode
60 seconds (READY to READY)
90 seconds (READY to
Run, Closed mode
READY)
Run, Pre-Dilute mode
60 seconds (READY to READY)
Auto-Shutdown (to STANDBY) Between 220 and 300 seconds
Table 1-9:
Complete Cycle Times — CELL-DYN 1700 and 1700CS
Aspiration Volumes — CELL-DYN 1700 and 1700CS
Approximate aspiration volumes (whole blood) are shown in Table 1-10.
Aspiration
Open mode
Pre-Dilute mode
Closed mode
30
40
450
Table 1-10: Aspiration Volumes
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Section 1
Collection Tube and Sample Volume
GENERAL INFORMATION
13 mm diameter x 75 mm high with a Hemoguard closure (maximum height is 100 mm for Closed
mode)
•
Minimum sample volume = 1 mL
Measurement Specifications — CELL-DYN 1700 and 1700CS
•
•
Impedance channel for both RBC and PLT
Hemoglobin Absorbance
Measurement specifications are listed in Tables 1-11 through 1-14.
Method
Dilution
Aperture Size
Data Collection
Aperture Impedance
1:285 of blood in diluent and lyse
100 µM (diameter) x 60 µM
(length)
Data collected in 256 channels
Each WBC channel = 1.758 fL
Table 1-11: WBC and Differential
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Section 1
Method
Dilution
Aperture Size
Data Collection
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GENERAL INFORMATION
Aperture Impedance
1:12,801 of blood in diluent
60 µM (diameter) x 70 µM (length)
256 channels for RBCs, each RBC channel
= 1 fL
256 channels for PLTs, each PLT channel
= 0.137 fL
Table 1-12: RBCs and PLTs
Method
Light Source
Wavelength
Dilution
Modified cyanmethemoglobin
LED
540 nm
1:285 of blood in diluent and
lyse
Table 1-13: HGB
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Section 1
Performance Specifications
GENERAL INFORMATION
Background Counts
The background values must meet the specifications given in Table 1-14.
WBC
<0.5 K/µL
RBC
<0.05 M/µL
HGB
<0.1 g/dL
PLT
<10.0 K/µL
Table 1-14: Background Counts
Linearity
Linearity specifications are determined by analyzing dilutions of a sample or commercially available
control material that contains no interfering substances and displays no suspect parameter flags.
Specifications are determined by taking multiple measurements on each dilution to minimize the effect
of imprecision.
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Section 1
GENERAL INFORMATION
The stated limits are determined by linear regression through the origin (0,0), assuring that the collected data throughout the linear reportable range does not exceed the stated Allowable Limits in
Table 1-15.
Parameter
WBC
RBC
HGB
MCV
PLT
MPV
Linear Reportable
Range
Allowable
Limits
(+/- or %)*
1.0 — 99.9 K/µL
+/- 0.4 or 3.0%
1.00 — 7.00 M/µL +/- 0.1 or 2.5%
2.5 — 24 g/dL
+/- 0.3 or 2.0%
50 — 200 fL
+/- 3.0 or 3.0%
10 — 999 K/µL
+/- 12.0 or 4.0%
5 — 20.0 fL
+/- 1.0 or 3.0%
Table 1-15: Linearity Specifications
*
Whichever is greater. Applies to actual mean value obtained in reference to the expected
value
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Section 1
Carryover
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GENERAL INFORMATION
Carryover is determined by running samples with high concentrations of WBCs, RBCs, HGB, and
PLTs. Each sample is run in triplicate followed by three background cycles. The percent carryover is
calculated using the following formula:
Carryover = (Background1 - Background3) x 100
(Sample3 - Background3)
Table 1-16 shows the carryover percent for WBC, RBC, HGB, and PLT in the Open, Pre-Dilute, and
Closed modes.
WBC
(K/µL)
Level
90.0
% Carryover < 1.0
RBC
(M/µL)
6.20
< 0.5
HGB
(g/dL)
22.0
< 0.8
PLT
(K/µL)
900
< 1.0
Table 1-16: Carryover — Open, Pre-Dilute, and Closed Modes
Within Sample Precision
Samples that are used to verify precision specifications should have results that fall within the laboratory's reference interval (normal range). These samples should not display any suspect parameter
flags.
Precision is a check on routine instrument operation. Tables 1-17 and 1-18 present the precision
specifications for the hemogram parameters for specimens run in the Open and Closed modes
respectively. The stated CV% in this table represents the instrument precision at a 95% confidence
level from N=20 replicate runs.
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Section 1
GENERAL INFORMATION
Precision specifications in the Pre-Dilute mode may have an increasedlevel of imprecision due to
operator technique.
Parameter
CV%
WBC
RBC
HGB
MCV
PLT
MPV
< 2.5
< 1.7
< 1.2
< 1.5
< 6.0
< 6.0
Table 1-17: Within Sample Precision of the Hemogram Parameters — Open Mode
Parameter
CV%
WBC
RBC
HGB
MCV
PLT
MPV
< 2.7
< 1.7
< 1.2
< 1.5
< 6.0
< 6.0
Table 1-18: Within Sample Precision of the Hemogram Parameters — Closed Mode
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Section 1
GENERAL INFORMATION
Precision specifications for the WBC Differential parameters are given in Table 1-19 as a 95% confidence limit for a range of values for each of the WBC subpopulations.
Cell Type
Range
95% Confidence
Level
Lymphocyte%
Mid
Granulocyte
18 — 57%
4 — 9%
36 — 77%
+/- 3.1%
+/- 1.6
+/- 3.5
Table 1-19: Precision of WBC Differential Parameters — Open and Closed Modes
Accuracy
Evaluation of the accuracy of the CELL-DYN 1700 and 1700CS in the Open mode is demonstrated in
Table 1-20. This data was computed from correlation analysis of data obtained from Method Comparison studies performed on approximately 100 whole blood samples analyzed against a reference
method. None of the samples used for the correlation studies exhibited any suspect parameter or suspect population flags.
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Section 1
Parameter
WBC
LYM#
MID#
GRAN#
RBC
HGB
HCT
MCV
RDW
PLT
MPV
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GENERAL INFORMATION
Range
Correlation Coefficient
0.5 — 96.5
0.1 — 94.5
0.0 — 11.4
0.1 — 40.8
1.48 — 6.47
4.2 — 18.2
12.5 — 55.3
63 — 113
10.8 — 27.6
11 — 939
6.4 — 15.4
> 0.98
> 0.92
> 0.60
> 0.92
> 0.98
> 0.98
> 0.98
> 0.98
> 0.92
> 0.98
> 0.92
Table 1-20: Whole Blood Accuracy Results — Open and Closed Modes
Bias
Bias in the case of the CELL-DYN 1700 is measured by the correlation coefficient, since the restricted
range of many hematology parameters precludes the use of the fitted linear regression equation to
ascertain the bias magnitude, such as is recommended in NCCLS Document EP9-T. Also note that
this restricted range, along with the known imprecision of the standard comparative methods, places
an upper limit on the degree of correlation that can be expected for several of the parameters.\
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Section 1
Performance Characteristics
GENERAL INFORMATION
Typical Precision
Performance characteristics provide a concise summary of system performance when operated under
normal laboratory conditions. The pooled precision values (CV%) for the Within Sample Hemogram
parameters, shown in Tables 1-21 and 1-22 for Open and Closed mode respectively, are based on
the analysis of data from replicate runs of N=20
Parameter
WBC
RBC
HGB
MCV
PLT
MPV
Typical
Precision
Within Sample
Range
4.4 — 9.0
3.78 — 5.60
12.2 — 15.9
78 — 97
179 — 420
7.4 — 11.5
CV%
2.1
1.2
1.0
1.0
3.2
4.6
Table 1-21: Typical Within Sample Precision Results — Open Mode
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Section 1
Parameter
WBC
RBC
HGB
MCV
PLT
MPV
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Typical
Precision
Within Sample
Range
4.0 — 11.4
3.92 — 5.47
11.7 — 16.7
73 — 100
165 — 408
7.3 — 12.4
CV%
2.2
1.4
1.0
1.0
3.2
4.2
GENERAL INFORMATION
Table 1-22: Typical Within Sample Precision Results — Closed Mode
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Section 2
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Section 2.
THEORY OF OPERATION
TABLE OF CONTENTS
2.1 SYSTEM OVERVIEW
2.2 SYSTEM DESCRIPTION
2.3 PURPOSE OF SYSTEM
2.4 SAMPLE PREPARATION
2.5 SAMPLE TRANSPORT
Open Sample Mode
Pre-Dilute Mode
Closed Sample Mode
Sample Dilution
2.6 PARTICLE DETECTION
2.7 PULSE AMPLITUDE TO PARTICLE SIZE RESPONSE
2.8 SIZE THRESHOLD AND CELL CHANNELIZATION
2.9 METERED VOLUME
2.10 RBC, WBC, AND PLT HISTOGRAM GENERATION
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Section 2
2.11 HEMOGLOBIN
THEORY OF OPERATION
LIST OF FIGURES
Figure 2-1 CELL-DYN 1700 Basic Block Diagram
Figure 2-2 Electrical Impedance Detection
Figure 2-3 Pulse Amplitude to Particle Size Response
Figure 2-4 Meniscus Detection
Figure 2-5 Histogram Generation
Figure 2-6 Simplified Hemoglobin Block Diagram
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Section 2
2.1 SECTION OVERVIEW
THEORY OF OPERATION
This section provides a brief review of the principles of operation for the CELL-DYN 1700, including
sample preparation, sample aspiration, the flow system, and the measurement process.
2.2 SYSTEM DESCRIPTION
The CELL-DYN 1700 is basically a particle counter dedicated to the electronic detection and measurement of blood cells contained in a sample of whole blood. Blood cells are particles which exhibit
the special quality of being electrical insulators. In whole blood, these particles are suspended in a
conductive medium commonly called plasma. It is these two natural electrical qualities that permit the
electronic measurement of the following:
•
•
•
The number of cells per unit of volume
The size of each cell
The size distribution of all cells contained in a sample.
A simplified diagram of a particle counter is shown in Figure 2-1. The major functions of an electronic
particle counter are as follows:
•
•
•
•
•
Sample transport (Flow System)
Particle detection (Transducer)
Pulse amplitude to particle size response (Amplifier)
Size thresholds (Discriminators) and cell channelization (A/D converter)
Sample volume metering (Metering System).
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2.3 PURPOSE OF SYSTEM
THEORY OF OPERATION
The purpose of this system is to convert the size of each detected particle to an electronic equivalent
signal. This signal is then processed to calculate the number of particles within a pre-selected size
range for a known sample volume. The displayed value represents the concentration of the sample in
cells per microliter (cells/µL).
Hemoglobin is measured by a separate colorimetric method. The absorbance, calculated from the
measured values of light transmission, is directly proportional to the concentration of hemoglobin.
A description of each major function of the instrument necessary to accomplish this task follows.
2.4 SAMPLE PREPARATION
A major disadvantage of whole blood measurement relative to electronic particle counting is the high
concentration of cells in whole blood. This problem is easily solved by controlled dilution. A prerequisite for electronic particle detection is low sample concentrations that will permit the existence of only
one particle in the sensing zone at any given time. Two or more cells in the sensing zone will be
detected as a single cell and result in a counting error. The high concentration of cells in whole blood
requires accurate dilution before electronic measurement can be attempted. When the dilution ratio is
known, the value measured by the instrument can be related to the whole blood value.
An obvious question is how much dilution of whole blood is required to satisfy the requirement of single cell detection in the sensing zone?
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It is the internal volume of the sensing zone that determines the ratio of dilution required. By calculation, the ideal dilution ratios for the CELL-DYN 1700 are as follows:
RBC:
1:12,801
WBC:
1:285
Whole Blood PLT: 1:12,801
These dilutions will reduce the coincidence of two or more cells in the sensing zone simultaneously,
but not eliminate it. Fortunately, this coincidence loss can be statistically predicted, based on sample
concentration, and coincidence corrected before display.
2.5 SAMPLE TRANSPORT
Open Sample Mode
The following events occur during WBC and RBC/PLT open sample processing:
1. When the Touch Plate is pressed, 30 ple Syringe from an open VACUTAINER®, held by
the operator, into the Sample Aspiration Probe.
2. As the probe, holding the sample, is raised, its exterior surface is cleaned by the Wash
Block.
3. The probe is then positioned in the Pre-Mixing Cup and the sample is dispensed with 7.5
mL of diluent. Blood and diluent are bubble-mixed in the cup. This dilution is referred to
as the pre-mix sample.
4. 100 raised, its exterior is cleaned by the Wash Block.
5. The probe is then positioned in the Mixing Chamber of the von Behrens RBC/PLT
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THEORY OF OPERATION
Transducer and the diluted sample is dispensed with 5 mL of diluent. The sample and
diluent are bubble-mixed in the chamber.
6. The remainder of the diluted sample from step 3 is transferred to the Mixing Chamber of
the von Behrens WBC Transducer. 1 mL of lyse is also deposited into the WBC
chamber. The sample and lyse are bubble-mixed.
7. In both the WBC and RBC Transducers, a vacuum draws the diluted sample from the
Mixing Chamber through the aperture plate into the Counting Chamber to obtain cell count
and histogram data.
8. WBC and RBC/PLT volume metering occurs simultaneously with WBC and RBC/PLT cell
counting.
9. Detergent in the HGB Flow Cell is measured to determine a reference.
10. Part of the sample from the WBC chamber is then sent to the HGB Flow Cell for HGB
measurement, pushing the detergent to the Waste System.
11. Samples remaining in the WBC and RBC/PLT Mixing Chambers, Metering Tubes, and
HGB Flow Cell are drained and sent to the Waste System.
12. The Pre-Mixing Cup, WBC/RBC chambers, and lines that carried the sample are washed
with diluent which is then flushed to the Waste System. The two Metering Tubes and
HGB Flow Cell are washed with detergent.
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THEORY OF OPERATION
There are two sources of pre-diluted solution. A third party vendor may supply it or the operator can
make a pre-diluted solution as described in Section 5: Operating Instructions, Subsection: Running Samples—Pre-Dilute Mode or Section 6: Calibration, Subsection: Pre-Dilute Method of the
CELL-DYN 1700 Operations Manual.
Whether the pre-diluted sample is supplied by a third party or prepared by the operator, the following
events occur:
1. In the RUN screen, press [PRE-DILUTE].
2. Pour the pre-diluted solution into the Pre-Mixing Cup and press the Touch Plate.
3. The instrument automatically performs additional dilutions and test measurements as
described in steps 4 through 12 of the Open Sample Mode processing sequence.
Closed Sample Mode
The following events occur during closed sample processing. For a more complete description of the
Closed Sample flow system, refer to the Closed Sample Option in Appendix B.
1. When an inverted, capped VACUTAINER® is placed in the VACUTAINER® holder of the
Closed Sample Assembly and the Closed Sample Touch Plate is pressed, a needle
pierces the VACUTAINER® cap.
2. 330 to wash out particles of previous diluent remaining in the needle, flow line, and
Sample Transfer Cup. The 330 t.
3. An additional 120 ing, displacing 120 Sample Transfer Cup.
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4. The 120 maining sample in the tubing (approximately 190 le Transfer Cup.
5. 30 ion Probe by the Sample Syringe.
6. Steps 2 through 12 of the Open Sample Mode flow sequence are repeated.
7. The remainder of blood sample in the tubing is pumped to the Sample Transfer Cup then
to the Waste System.
8. Diluent is pumped into the Sample Transfer Cup to soak the cup, then flushed to the
waste system. Diluent is again pumped to the cup and backflushed through the flow line
to the needle and waste well to wash out these components, then pumped to the Waste
System.
Sample Dilution
A 30 µL sample of whole blood is drawn into the Sample Probe and mixed with 7.5 mL of diluent to
make the primary dilution. A second 100 µL is then aspirated from the primary dilution to make the
secondary 1:12,801 RBC/PLT dilution. The primary dilution is then mixed with 1 mL of lyse to complete the 1:285 WBC/HGB dilution. The vacuum system transports the primary and secondary dilutions through the WBC and RBC apertures and HGB Flow Cell.
The flow system is then flushed and made ready for the next sample.
2.6 PARTICLE DETECTION
A transducer employing the electrical impedance principle is used for detection. This function performs the conversion of the physical properties of a detected cell to an electronic equivalent signal.
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Figure 2-2 depicts this principle. An aperture of defined diameter and length separates the flow of a
constant current between an inner and outer electrode. Conduction is provided by an electrolyte. In
this case, the electrolyte is buffered saline.
The electrical current is supplied by a constant current source and it continues at a constant rate in the
absence of a particle (cell) within the confines of the aperture. Therefore, there are no interruptions to
this current flow and no signal appears at the output of the amplifier.
Consider the passage of a blood cell, an insulator, through the aperture. The passage of the cell
caused by the differential pressure between the isolated tanks will cause a momentary increase in
impedance, which is directly related to the volumetric size of the cell. Constant current is maintained
by a proportional increase in voltage. The charge and discharge of the coupling capacitor induces a
signal into the inverting input of the amplifier. The output of the amplifier produces an instantaneous,
amplified electrical pulse. The amplitude represents the volumetric size of the detected cell.
2.7 PULSE AMPLITUDE TO PARTICLE SIZE RESPONSE
The continuous passage of cells through the aperture's sensing zone produces a pulse train at the
output of the amplifier. The gain control of the amplifier calibrates the sizing function of the instrument
by establishing a known relationship between the mean size of the cells and the mean pulse amplitude of the signal. This linear response is depicted in Figure 2-3.
2.8 SIZE THRESHOLD AND CELL CHANNELIZATION
Figure 2-1, shown earlier, is a basic block diagram of the measurement and metering circuitry for
RBC, WBC, and PLT.
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+ VOLTS
AMBIENT
PRESSURE
VACUUM
CONSTANT CURRENT
GAIN
APERTURE
SIGNAL OUT
AMPLIFIER
SAMPLE
DETERGENT
TRANSDUCER
Figure 2-2 Electrical Impedance Detection
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The output of the amplifier is routed to the input of the coarse discriminator and switched input of an A/
D converter. If the amplitude of an individual cell pulse (analog signal) is within a pre-selected range,
the coarse discriminator will close the switch and place the cell pulse on the A/D converter input. The
A/D converter then converts the cell pulse to a 9-bit digital word that is directly proportional to the peak
amplitude. This 9-bit word (cell A/D data) is sent to the main computer, where it increments an individual size channel (memory location).
There are 256 size channels for each parameter RBC, WBC, and PLT.
Upon completion of the sample cycle, this data is used to generate counts, histograms, and percentage results for final display.
2.9 METERED VOLUME
The measurements require a known, repeatable sample volume. The instrument performs this function by optical detection of the leading edge of a liquid column (meniscus), as depicted in Figure 2-4.
The light transfer efficiency between an IR (infrared) light source and a photo transistor is controlled
by the optical characteristics of a glass metering tube in the light path. In the absence of liquid, as
shown in State A, the metering tube contains air and reduces the transfer of light by the refraction of
the glass walls and the density of the air within the glass tube.
With reference to State C, the metering tube is filled with liquid. The level of refraction is reduced by
an increase in optical density of the liquid, and a small increase in light transfers the results.
A third state will momentarily occur during the transition of the meniscus through the light path. As
shown in State B, the light transfer efficiency is greatly reduced when the light path is deviated by
refraction as well as reflection qualities of the concave meniscus. It is this phenomenon that serves
as a leading edge detector.
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When two detectors are placed along a fixed length of a precision bore metering tube, the volume of
sample can be measured by sensing a start count at the first detector and a stop count at the second
detector.
2.10 RBC, WBC, AND PLT HISTOGRAM GENERATION
As stated earlier, each parameter has 256 channels available. The width of each channel is as follows:
RBC
WBC
PLT
=
=
=
1.00 cubic microns
1.758 cubic microns
0.137 cubic microns
The RBC will be used as an example since it has a 1:1 relationship.
Figure 2-5 illustrates a smoothed RBC histogram and an enlarged view of the raw counts per channel
of the peak portion of the histogram (section “A”).
If we compare section “B” with section “A”, we can see the relationship of channel data to the actual
histogram shape. The raw counts increase, with volume, on the leading edge and decrease on the
trailing edge.
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THEORY OF OPERATION
The MCV is derived by calculating the weighted average of the number of red blood cells in a specified channel range as follows: (1) the number of red blood cells per channel is multiplied by the channel number then the results of each multiplication are summed, and (2) the sum calculated above is
then divided by the sum of the red blood cells in the specified channel range.
From the data accumulated in all channels we can also derive RBC count and Hematocrit.
WBC and PLT histograms are generated in the same manner and are used in various equations to
derive other calculated parameters.
A description of all parameters is contained in the CELL-DYN 1700 Operations Manual.
2.11 HEMOGLOBIN
A simplified hemoglobin system is shown schematically in Figure 2-6. The concentration of hemoglobin in the prepared sample is measured in grams per deciliter. This concentration is proportional to
the absorbance of the light in the green, 540 nanometer wavelength region.
A clear reference solution (detergent) is first measured in the HGB Flow Cell. A prepared sample containing hemoglobin is measured next. Hemoglobin concentration is determined by subtracting the
logarithm of the voltage of the measured sample from the logarithm of the voltage of the clear reference solution.
A light path through the transparent flow cell is formed from the light source, a 540 nanometer interference filter, and a photo detector.
The output current from the photo detector, which is proportional to the light energy received, is amplified by the current-to-voltage amplifier and provides an output signal.
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THEORY OF OPERATION
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Section 3.
SYSTEM DESCRIPTION
Table of Contents
3.1 SECTION OVERVIEW
3.2 SYSTEM SUB-ASSEMBLIES
Flow Panel
Fluid Power Supply
Reagent Inlet Panel
Syringe Assembly
Electronics Card Cage Assembly
CRT and Keyboard
Power Supply Module
3.3 MAJOR SUBSYSTEM DESCRIPTIONS
Data Interface and Control Subsystem
Measurement Subsystem
Solenoid and Motor Subsystem
User Interface Computer Subsystem
Data Link Adapter (DLA)
IDE Controller and I/O Board
Input/Output Board
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Super VGA Board
Computer Monitor
AC and DC Power Distribution Subsystem
List of Tables
Table 3-1 PC Motherboard Configuration
List of Figures
Figure 3-1 Data Interface and Control Board Diagram
Figure 3-2 Measurement Block Diagram
Figure 3-3 Solenoid and Motor Drive Block Diagram
Figure 3-4 User Interface Computer
Figure 3-5 Power Distribution Block Diagram
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Section 3
3.1 SECTION OVERVIEW
THEORY OF OPERATION
This section contains a system overview, as well as information on the major subsystems. For a more
detailed description of the CELL-DYN 1700 parameters, reagents, specifications, and operation, refer
to the CELL-DYN 1700 Operations Manual.
3.2 SYSTEM SUB-ASSEMBLIES
The CELL-DYN 1700 is divided into the following major sub-assemblies:
•
•
•
•
•
•
•
Flow Panel
Fluid Power Supply
Reagent Inlet Panel
Syringe Assembly
Electronics Card Cage
CRT and Keyboard
Power Supply Module
Flow Panel
The Flow Panel consists of the majority of tubing and hardware for sample processing.
Fluid Power Supply
The Fluid Power Supply contains the vacuum and pressure pumps, waste bottles, and associated
solenoids and hardware.
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Reagent Inlet Panel
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THEORY OF OPERATION
The Reagent Inlet Panel provides connections for incoming reagents and outgoing waste. Solenoids
for lyse, detergent, and diluent are also mounted on this panel.
Syringe Assembly
The Syringe Assembly includes the Sample Syringe for aspirating samples, the Diluent Syringe for
supplying diluent throughout the flow system, and the Lyse Syringe for dispensing lyse during the
HGB measurement process.
Electronics Card Cage Assembly
The electronics assembly provides command and control signals for the various electronic components of the instrument. This assembly contains the Cell Count Module, Signal Processor Module,
Main Amplifier Module, and the Device Control Module.
CRT and Keyboard
The CRT provides visual data display and the keyboard provides data input by the operator.
Power Supply Module
The Linear (main) Power Supply Module provides power directly to the Cable Distribution Module, the
Pre-Amplifier Module, and analog circuit boards. The PC Power Supply provides power to the Power
Distribution Module which in turn provides power to the PC Motherboard and digital logic boards. The
24V (regulated) Switcher provides power for the stepper motors via the Power Distribution Module
and Motor Processor Module.
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3.3 MAJOR SUBSYSTEM DESCRIPTIONS
THEORY OF OPERATION
To aid in understanding the overall system, the electronic modules are divided into the following major
functional subsystems:
•
•
•
•
•
Data Interface and Control Subsystem
Measurement Subsystem
Solenoid and Motor Drive Subsystem
User Interface Computer Subsystem
AC and DC Power Distribution Subsystem
Data Interface and Control Subsystem
The purpose of this subsystem is to interface the user data, control data, and system status data in
the system. This data is connected via four independent data busses:
•
•
•
•
UIC/CCM (User Interface Computer/Cell Count Module)
CCM/DCM (Cell Count Module/Device Control Module)
DCM/CDM (Device Control Module/Cable Distribution Module)
DCM/MPM (Device Control Module/Motor Processor Module)
Refer to Figure 3-1 for a diagram showing the data connections.
When power to the instrument is turned ON, the system operating software is loaded from the hard
disk into RAM on the UIC (User Interface Computer). The UIC then uses various handshaking signals
and data bytes to communicate with the CCM (Cell Count Module).
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The CCM functions as the master controller with all system functional commands residing in firmware
(PROM). The CCM sends control data and receives status data from the DCM (Device Control Module).
The DCM provides current control to the von Behrens RBC and WBC Transducers and the two metering boards and serves as the system analog voltmeter for use in converting the HGB signal. Data is
written and read via the DCM/CDM and DCM/MPM data busses.
The CDM (Cable Distribution Module) acts as controller for the solenoids and also interfaces data
from various system sensors.
The MPM (Motor Processor Module) acts as controller for all Stepper Motor Drive printed circuit
boards.
Measurement Subsystem
The measurement subsystem provides detection, amplification, and processing of the signals from
the von Behrens RBC/PLT Transducer, von Behrens WBC Transducer, and HGB Flow Cell. RBC/
PLT and WBC metering is also included in this subsystem.
Refer to Figure 3-2 for a diagram of the measurement process.
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The PAM (Pre-Amplifier Module) supplies constant current for the von Behrens RBC/PLT and WBC
Transducers and HGB LED voltage.
The RBC/PLT and WBC cell pulses are input to the PAM where they are amplified and routed to the
MAM (Main Amplifier Module).
When the MAM receives signals from the RBC/PLT and WBC, the following occurs:
• The RBC/PLT signal is amplified and split into independent RBC and PLT signals.
• The WBC signal is amplified and sent to the SPM (Signal Processor Module).
• The PLT signal is sent to the SPM.
• The RBC signal is routed to the input of the SPM and the cell editing circuitry.
• Cell editing is performed on the RBC signal to eliminate invalid RBC pulses.
A detailed description of cell editing is contained in Section 4, Circuit Descriptions.
The RBC/PLT and WBC signals are accepted by the SPM. The SPM discriminates cell size by converting pulse height to a proportional digital value. The amplitude of each valid pulse is measured by
a fast A/D then sent across the data bus to the CCM.
The A/D data for RBC, PLT, and WBC are individually divided by the CCM into 256 discrete size
channels. The cell count in each channel is accumulated in discrete memory locations and is used to
generate count data, percentage data, and histogram data for RBC, PLT, WBC, and other parameters.
Signals from the upper and lower detectors on the RBC/PLT and WBC metering modules are converted to TTL levels by comparators on the CDM. The signals are then routed through the DCM to the
CCM where they are used to control RBC/PLT and WBC sample timing.
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The HGB analog signal from the HGB Flow Cell is input to the PAM where it is amplified and routed to
the DCM. The HGB signal is then measured and converted to a digital format by a voltmeter—A/D
converter. The digital value is then sent, via the CCM/DCM data bus, to the CCM for final processing.
Solenoid and Motor Subsystem
Solenoid control commands reside in firmware on the CCM. These commands are sent to the DCM
and then to the CDM where they are multiplexed to the appropriate SDM (Solenoid Drive Module).
The SDM then provides the current to open and close individual drive solenoids.
Stepper Motor commands are handled in much the same manner as described above. However, the
final multiplexing of the Stepper Drive printed circuit boards is controlled by the MPM.
There are two pressure pump modules and one vacuum pump module in the CELL-DYN 1700.
These modules are described as follows:
1. A pressure pump provides air to bubble-mix samples in the Pre-Mixing Cup and the
mixing chambers of the von Behrens RBC/PLT and WBC Transducers. A pressure
regulator provides 0.5 psi for this process.
2. An unregulated pressure pump provides air to push waste from the waste bottles inside
the instrument to the waste container attached to the instrument and to apply back
pressure to clear the apertures in the von Behrens RBC/PLT and WBC Transducers.
3. 8” Hg vacuum container, vacuum sensor, and vacuum pump supply a constant vacuum to
the entire system to transport diluent, detergent, and lyse throughout the flow system and
to maintain a constant vacuum to the RBC/PLT and WBC metering tubes. A vacuum
regulator provides constant pressure to both metering tubes.
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Refer to Figure 3-3 for a diagram of the solenoid and motor drive connections.
User Interface Computer Subsystem
The User Interface Computer is a PC-AT type 386 or 486 computer. The 386 board is manufactured
by DFI, and the 486 board is manufactured by Micronics. The CPU operates at a clock speed of
between 25 ( 66 MHz depending on the board and configuration.
Table 3-1 gives a brief description of the motherboard.
CPU
BIOS
ROM
RAM
EPROM
I/O Functions:
Table 3-1:
Intel 386SX, 25/33/40 MHz or
Intel 486DX, 33/66 MHz
AMI BIOS
128 KB
2 MB onboard RAM memory (SIMMs)
2 pieces of 27256 ROM BIOS
AT keyboard interface; 8742 keyboard chip
Pin # Connector
1
keyboard clock
2
keyboard data
3
not connected
4
ground
5
+/- 5V
PC Motherboard Configuration
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Slots
CMOS RAM
Display interface
1.44 MB FDD
270 (or higher) MB HDD
6 16-bit ISA slots
64 bytes for RTC
Super VGA
Hard/Floppy disk interface
IDE
Peripheral devices
Table 3-1:
THEORY OF OPERATION
PC Motherboard Configuration (Continued)
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In addition to the motherboard, the User Interface Computer consists of the following components:
1. Data Link Adapter to interface with Cell Count Module
2. IDE Controller (for floppy and hard disk drives) and I/O board
3. Input/Output board
4. Super VGA board
5. Computer monitor
Figure 3-4 illustrates the major components of the User Interface Computer.
Data Link Adapter (DLA)
The Data Link adapter provides interfaces from the PC motherboard to both the Cell Count Module
and the instrument key panel. Refer to Section 4 for a detailed description of the DLA.
IDE Controller and I/O Board
The IDE (Integrated Device Electronics) controller and I/O (Input/Output) board contains the interface
to both the hard and floppy disk drives, one serial port, and one parallel port. The cable to the hard
disk drive is a 40-pin connector and the cable to the floppy disk drive is a 34-pin connector. The board
must be jumpered so that the serial port is COM1 and the parallel port is LPT1. If applicable, the
graphics printer must be connected to LPT1.
NOTE: If a LIS (Laboratory Information System) is installed, it must be connected to the COM1 port.
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Input/Output Board
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THEORY OF OPERATION
The standard I/O board is a PC-AT type board with a serial port and parallel port. The serial port must
be jumpered to COM2 and the parallel port must be connected to LPT2. If applicable, the ticket
printer must be connected to LPT2.
Super VGA Board (SVGA)
The super VGA board operates in standard VGA mode. It has a 16 color capability and a standard
15-pin VGA connector. It must be plugged into a 16-bit slot. The board has no adjustment.
Computer Monitor
The computer monitor is a custom 14” color monitor from ELECTROHOME. It operates in 16 color
mode with a resolution of 640 x 480. The monitor has special shielding and the display adjustment
controls are mounted on the front directly below the screen. The controls from left to right are contrast,
brightness, height adjustment, screen position adjustment, and screen width adjustment.
The monitor has a built-in cable that is attached to the SVGA board. AC power comes directly from
the Linear (main) Power Supply.
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AC and DC Power Distribution Subsystem
THEORY OF OPERATION
The AC and DC functions of the power supply are handled by the PDM (Power Distribution Module).
The AC line is routed through a RF filter to a set of power selector switches which accommodate 100,
120, 220, and 240 VAC (50/60 Hz). The AC board routes 115 VAC to the Linear (main) Power Supply
and fans. The +12 VDC and +24 VDC used for solenoid drivers are fused at the Linear Power Supply.
None of these voltages are adjustable on the power supply.
NOTE: The +12VDC unregulated = 16.5V nominal. The +24VDC unregulated = 34V nominal.
Refer to Figure 3-5.
The following voltages are supplied to the instrument by the Linear Power Supply:
• +15VDC (regulated) and -15VDC (regulated) to the analog electronics boards
• +100VDC (regulated) to the Pre-Amp Module
• +24VDC (unregulated) to close the solenoids
• +12VDC (unregulated) to hold the closed solenoids in position
The CPU Power Supply provides the following voltages which are routed to their final destinations by
the Power Distribution Module:
• +5VDC (regulated) to the PC motherboard
• +5VDC (regulated) to the digital logic boards
• +5VDC (regulated) to the Motor Processor Module
• +12VDC (regulated) to disk drives
• – 5VDC (regulated) to the PC motherboard
• – 12VDC (regulated) to the PC motherboard
The 24V Switching Module provides:
• +24VDC (regulated) to the Motor Processor Module via the Power Distribution Module
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THEORY OF OPERATION
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Section 4.
CIRCUIT DESCRIPTIONS
Table of Contents
4.1 SECTION OVERVIEW
4.2 PRE-AMPLIFIER MODULE (PAM)
4.3 MAIN AMPLIFIER MODULE (MAM)
4.4 SIGNAL PROCESSOR MODULE (SPM)
4.5 CELL COUNT MODULE (CCM)
Microprocessor Section
System Clock
6809E Microprocessor Support Circuits
PROM
RAM
VIA (Versatile Interface Adapter)
DMA (Direct Memory Access) Section
Pulse Height Memory (PHM)
Cell Counters
DMA Timing and Control
Interface to Microprocessor Bus
4.6 METERING MODULE
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4.7 DEVICE CONTROL MODULE (DCM)
4.8 CABLE DISTRIBUTION MODULE
4.9 SOLENOID DRIVER MODULE (SDM)
4.10 MOTOR PROCESSOR MODULE (MPM)
4.11 STEPPER DRIVE PRINTED CIRCUIT BOARD
4.12 PRESSURE/VACUUM REGULATOR MODULE
4.13 LINEAR POWER SUPPLY MODULE
4.14 PC POWER SUPPLY MODULE
4.15 24V SWITCHING MODULE
4.16 PUMP RELAY MODULE (PRM)
4.17 USER INTERFACE COMPUTER (UIC)
4.18 DATA LINK ADAPTER (DLA)
Interface to CCM
Interface to Key Panel
CIRCUIT DESCRIPTIONS
List of Figures
Figure 4-1 Cell Pulse Classifications
Figure 4-2 MAM (Main Amplifier Module) Block Diagram
Figure 4-3 SPM (Signal Processor Module) Block Diagram
Figure 4-4 DLA (Data Link Adapter) Block Diagram
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4.1 SECTION OVERVIEW
CIRCUIT DESCRIPTIONS
This section contains a description of the circuitry for the following printed circuit boards:
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Pre-Amplifier Module (PAM)
Main Amplifier Module (MAM)
Signal Processor Module (SPM)
Cell Count Module (CCM)
Metering Module
Device Control Module (DCM)
Cable Distribution Module (CDM)
Solenoid Driver Module (SDM)
Motor Processor Module (MPM)
Stepper Drive Printed Circuit Board (SDP)
Pressure/Vacuum Regulator Module (PVRM)
Linear Power Supply Module
PC Power Supply Module
24V Switching Module
Pump Relay Module (PRM)
User Interface Computer (UIC)
Data Link Adapter (DLA).
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4.2 PRE-AMPLIFIER MODULE (PAM)
CIRCUIT DESCRIPTIONS
Refer to Figure 3-2 in Section 3.
The PAM performs the following functions:
1. Provides RBC/PLT and WBC constant current
2. Amplifies the initial RBC/PLT, WBC, and HGB signals
Constant current bias (100VDC), switched by U8 and Q3, is routed to U5 which supplies constant current to the von Behrens RBC/PLT Transducer. Two independent RBC/PLT current levels are controlled by U8 and Q2, and PLT current is adjusted by R21.
U9 and associated circuitry provide constant current for the von Behrens WBC Transducer. R35
adjusts WBC constant current. WBC guard voltage is supplied by U6.
U4 and U7 provide initial amplification of the RBC/PLT and WBC Transducer signals.
The output of the HGB Flow Cell is amplified by U1 and U2. HGB offset is adjusted by R5 and HGB
gain is adjusted by R12.
4.3 MAIN AMPLIFIER MODULE (MAM)
Refer to Figure 3-2 in Section 3 and Figures 4-1 and 4-2 in this section.
The Main Amplifier Module consists of the following major circuits:
1. WBC differential amplifier and main amplifier
2. RBC/PLT differential amplifier and main amplifier
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Section 4
3. RBC final stage amplifier
CIRCUIT DESCRIPTIONS
4. PLT final stage amplifier
5. Pulse editing circuit.
The WBC signal from the WBC pre-amp is received by the WBC differential amp, amplified by the
main amplifier then DC-restored by U4 before the final buffer amplifier. The signal then goes to the
Signal Processor Module (SPM).
The RBC/PLT composite signal from the RBC/PLT pre-amp is received by the RBC/PLT differential
amplifier, is amplified by the MAM, then DC-restored by U8 and split to the RBC and PLT final stage
amplifiers.
The RBC final stage amplifier has two amplification levels which are selected by the aperture current
select signal. The purpose of dual amp levels is to maintain a RBC output signal that is below saturation in the high current mode but that still has adequate amplification during the low current mode.
Both amplifier levels are adjustable.
The PLT final stage is adjustable and should be set to a level 3.3 times greater than the RBC level.
Pulse editing is a technique that enables the MAM to distinguish between normal and abnormal cell
signals as shown in diagram (A) of Figure 4-1.
Normal cell signals are generated by cells that pass through or near the center of the aperture in a
straight line. Abnormal cell signals can be generated in several ways:
•
•
•
a cell tumbling in the orifice (diagram (B) in Figure 4-1)
more than one cell passing through the orifice at the same time (diagram (C) in Figure 4-1)
cells that pass near the edge of the orifice (diagram D in Figure 4-1).
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CIRCUIT DESCRIPTIONS
To determine which cells are valid, the height (H) of each cell pulse is measured and compared to the
area (A) under the signal envelope. In normal cell signal the area is less than the height (A < H).
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CIRCUIT DESCRIPTIONS
Figure 4-2 shows a block diagram of the pulse editing circuit. The RBC analog signal from the final
buffer amplifier, U2, goes to pin 3 of U15 and then to the following places:
1. Peak Detector
2. Sample/Hold
3. Discriminator
4. Integrator
The peak detector, U19, signals the analog switch in the sample/hold circuit, U18, to hold the cell
peak until the flip flop, U21, is reset. The output of the sample/hold goes through a buffer amp to the
non-inverting input of comparator U17. The DC level represents the signal height "H" in the equation.
The discriminator, U17, distinguishes between noise and cell signals. Each time a cell signal is
detected, the output of the discriminator goes high, closing an analog switch, allowing only cell pulses
to be integrated by U25.
The integrator, U25, is used to determine the area under the signal envelope of each cell pulse. The
output of the integrator and buffer amp is kept at a DC level and represents the area "A" in the equation. The signal goes to the inverting input of comparator U17.
When the DC level of "H" is greater than the DC level of "A" the output of the comparator will be high.
The output of the comparator goes to one input of an AND Gate, U22. The other input is tied to the
output of the first one shot, U20. When the comparator is high, indicating a valid cell signal, and the
one shot pulses high, the output of U22 will pulse low, generating a cell strobe. The cell strobe signal
goes to the Signal Processor Module (SPM) which is shown in Figure 4-3.
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CIRCUIT DESCRIPTIONS
After the cell strobe pulses low, a second one shot pulses and resets the flip flop U21 and discharges
the integrator capacitor on U28.
4.4 SIGNAL PROCESSOR MODULE (SPM)
Refer to Figure 3-2 in Section 3 and Figure 4-3 in Section 4.
The SPM consists of the following main sections:
•
•
•
•
•.
•
•
WBC Sample and Hold (S/H)
RBC S/H
PLT S/H
WBC or RBC/PLT Analog Switch
RBC or PLT Analog Switch
RBC or PLT Selector
A/D Convector
Since one A/D converter is used, a time sharing method is required to convert independent RBC, PLT,
and WBC signals. A six microsecond pulse (CELL CLK), generated on the CCM board, is used to
multiplex these independent signals.
The peak amplitudes of the WBC, RBC, and PLT pulses are stored in capacitors C19, C27, and C32,
respectively. When CELL CLK is high, analog switch (U2) transfers the WBC voltage to the analog
input of A/D converter (U1), and when CELL CLK is low, the RBC or PLT voltage placed on the input.
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CIRCUIT DESCRIPTIONS
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CIRCUIT DESCRIPTIONS
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CIRCUIT DESCRIPTIONS
The RBC or PLT determination is controlled by the RBC/PLT signal which, in turn, is controlled by the
PLT Low (U28-13) and PLT HI (U28-14) discriminators and associated circuitry. When the thresholds
of both are exceeded, indicating an RBC pulse, the RBC/PLT signal is high and the RBC S/H voltage
is converted. When only the low threshold is exceeded, indicating a PLT pulse, the PLT S/H voltage is
converted.
When CELL CLK changes state, a conversion command signal is generated which starts the A/D conversion, and forces the end-of-conversion signal (EOC) high. Upon the completion of the A/D conversion, EOC returns low, and EOC, RBC/PLT, and 9-bits of A/D conversion data are sent to the CCM.
4.5 CELL COUNT MODULE (CCM)
Refer to Figure 3-2 in Section 3.
The primary function of the CCM is to count the cell pulses presented to it by the SPM (Signal Processing Module). The CCM can be otherwise considered a general purpose microprocessor-based
process controller. Thus, the CCM consists of two main sections:
1. Cell counting logic
2. Microprocessor related circuitry
The cell counting section is a DMA (Direct Memory Access) approach to the counting and storing of
cell pulses per channel into the histogram data storage memory. At the end of the sampling interval,
the CCM program then reads out the accumulated counts per channel in the Pulse Height Memory
(PHM).
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Section 4
The main sections of the CCM are:
CIRCUIT DESCRIPTIONS
1. Microprocessor Section
a. System clock
b. 6809E microprocessor & support circuits
c. EPROM for program storage
d. RAM for program data storage
2. VIA (Versatile Interface Adapter)
a. Real time clock
b. Interface to UIC
c. LED Function & Counting Control
3. DMA (Direct Memory Access) Section
a. Pulse Height Memory (PHM)
b. Cell Counter
c. DMA timing and control Interface to microprocessor bus
Microprocessor Section
System Clock
The CCM uses an 8 MHz oscillator (U10) that is divided by eight by a Johnson counter (U20) to provide 1 MHz system clocks for the 6809E microprocessor. The signals E and Q are provided to the
6809E by the Johnson counter. VUA (Valid User Address) is provided to pin 10 on the motherboard.
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6809E Microprocessor Support Circuits
CIRCUIT DESCRIPTIONS
The data and address buffers of the 6809E are buffered by an LS640 (U33) inverting bi-directional
buffer for the data bus and two LS244 octal buffers (U34, U35) for the address bus. Address decoding is done by four LS139 2 to 4 decoders (U23, U21).
A 555 timer (U12) is used for the 6809E power-up reset. The 6809 may also receive an external reset
signal via pin 14 on the edge connector. This is the system-wide reset.
PROM
The EPROM used on the CCM (U37) is a 27C256, for 32K by 8 of program storage. A strappable
jumper selects the EPROM type.
RAM
The program RAM used on the CCM (U36) is a HM6264 (or equivalent), for 8K by 8 of program data
storage. A strappable jumper selects the RAM type. Jumper J5 must be set to 8K.
VIA (Versatile Interface Adapter)
The LSI (Large Scale Integration) interface used on the CCM is a 6522 VIA (U13). This device performs a number of functions, as described below:
1. CCM Real Time Clock
One of the two 16-bit timer/counters in the VIA is used for the CCM real time clock. This time
base is always programmed to 1 millisecond (in current applications); it presents a repetitive
FIRQ interrupt to the 6809E. All process control functions, e.g., flow system timing, stepper
motor motions, sensor scanning rates, etc., are based on this timer. There should always be a
1 KHz frequency at test point TP5.
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2. Interface to UIC
CIRCUIT DESCRIPTIONS
The VIA is also used as an interface to the User Interface Computer (UIC). This interface uses
the A-side of the VIA for an 8-bit multi-byte parallel data transfer, with VIA signals CA2 and CA1
used as strobe and acknowledge for each byte sent/received.
The handshake for data block transfers is controlled by REQ2 and REQ1. In normal system
operation, the UIC will periodically set REQ2 low to request CCM data/status, and the CCM will
answer by setting REQ1 low and keeping it low until all bytes (if any) have been sent.
3. LED Function & Counting Control
The two LEDs for REQ1 and REQ2 (DS6, DS7) indicate the communication activity. They
directly relate to the hi/lo state of REQ1 and REQ2. When DS6 is on, it indicates that REQ1 is
active; when DS7 is on, it indicates that REQ2 is active.
The LEDs DS1 through DS5 are entirely under program control. Their current use is as follows:
a. The CCM green LED (DS1) should always be on after the CCM has successfully completed its internal power-on self-check diagnostics, otherwise there is a fundamental
CCM fault.
b. The LEDs CER and CEW (DS2, DS3) indicate the state of the CCM firmware
generated signals CER (count enable red) and CEW (count enable white). These
signals control cell counting. When the LED is on, the DMA cell counting circuitry is
active.
The LED DS4 is programmed to give a rough indication of the rate at which pulses are being
generated by the SPM. The LED DS5 is used to indicate that a self test is in progress.
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DMA (Direct Memory Access) Section
CIRCUIT DESCRIPTIONS
Pulse Height Memory (PHM)
Two HM6116 2K by 8 static memory devices (U15 and U14) are used to store the pulse height
counts. The two memory devices are electrically set up as 2K bytes by 16 bits of addressable memory. Furthermore, this memory is divided into four functional blocks of 512 16-bit words. In normal
operation, these blocks hold the WBC, RBC (low current), Platelet, and RBC (high current) counts per
channel.
The PHM is unique in 2 important ways. First, the CCM program can only read the memory or clear
the memory, it cannot store values into the memory (except for zero, by clearing it). Second, the CCM
program is blocked from reading the PHM memory while a DMA is in progress.
The DMA circuit, on the other hand, can read and write the memory directly, but it can only transfer
data to and from the
16-bit cell pulse counter.
Read/write control of the PHM is performed by an LS158 (U18), which acts as a DMA / MPU address
selector.
Cell Counters
The cell count values stored in the PHM are incremented by the four 4-bit counters (U29, U28, U27,
U26). These counters are cascaded and employed as a 16-bit pre-settable synchronous counter.
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DMA Timing and Control
CIRCUIT DESCRIPTIONS
In order to process cell pulse data in a synchronous manner, a lower frequency cell-clock is generated
from the 1 MHz clock. An LS92 (U6) is used to divide the 1 MHz by 12. The output of this counter
(83.3 KHz) is presented to the SPM and is also used internally by the CCM. In effect, this synchronizes the pulse processing A to D circuitry on the SPM with the pulse counting circuitry on the CCM.
An RBC/PLT or WBC cell pulse is processed within a 6 microsecond (µs) time frame. WBC pulses are
handled when the cell clock signal is high; RBC, or PLT pulses are processed when the cell clock signal is low. This 6 µs time includes the SPM A to D conversion time (about 1.8 to 2.4 µs) and an intentional SPM delay of 1 µs before the start of conversion.
A pulse height (the A to D output) produced by the SPM is strobed into an LS374 latch (U1) on the
CCM by the SPM's EOC (End-Of-Conversion) signal (pin 9 on J2 and TP7). Given that CER or CEW
is active, the arrival of this EOC signal also starts a CCM cell processing READ/COUNT/WRITE DMA
sequence that proceeds as follows:
1. The signal DMR (Direct Memory Read) is generated by an LS175 F/F (U2). This is a 1 µs
pulse that is used to read the PHM data at the address specified by the pulse height and
the SPM RBC/PLT signal. This data is then loaded into the LS569 counters with a 125
nanosecond (ns) pulse. The signal DMW (Direct Memory Write) is then generated by
another LS175 F/F (U2). The DMW signal is a 1 µs pulse that is used to control the data
write-back.
2. While DMW is high, a 500 ns pulse is generated to increment the 16-bit counter, and thus
count the cell having this particular size.
3. The output of the counters is enabled onto the internal PHM data bus. The PHM Write
Enable signal (WE) is brought low to strobe the output of the counter back into the PHM at
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CIRCUIT DESCRIPTIONS
the latched address specified by the pulse height and the SPM RBC/PLT signal.
Interface to Microprocessor Bus
The CCM firmware provides an address to the PHM via two LS244s (U16, U17) and reads the 16-bit
PHM output data via two LS374s (U30, U31).
It should be noted that only 15 bits are used for the cell count. Thus the CCM is designed to handle a
maximum of 32,767 counts (7FFF hex) in any one channel.
4.6 METERING MODULE
Refer to Figure 3-2 in Section 3.
Both RBC and WBC count times are measured by a precision glass metering tube in conjunction with
two infrared optical detectors (CR3/Q1) and (CR4/Q2). The volume of liquid within the metering tube
between the upper and lower optical switches is approximately 200 µL. This ensures that a precise
amount of sample is aspirated each sample cycle.
When there is no obstruction of light, the output (TP1-TP2) of the upper and lower detectors is approximately 0.630 volts.
During the sample cycle, an inverted meniscus travels down the metering tube. As it passes the
upper detector, the curved shape bends the light away from the photo-transistor which causes the
output to pulse high (approximately 3.8 volts) and the computer to start the sample count. When the
meniscus passes the lower detector, the output again pulses high, causing the computer to stop the
sample count.
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CIRCUIT DESCRIPTIONS
The LED drive and output amplifiers are connected in a positive feedback configuration. Positive
feedback, supplied by a diode and 10K resistor, compensates for changes in light transfer and holds
the outputs at a constant low level. The time constant of a 1M resistor and a 22 µf capacitor slows the
response time of the feedback loop, thus ensuring adequate pulse width (>20 ms) when the meniscus
passes.
LEDs (DS1-DS4) provide background illumination for the metering tube.
4.7 DEVICE CONTROL MODULE (DCM)
Refer to Figure 3-3 in Section 3.
The DCM performs the following major functions:
1. System Analog Voltmeter
2. Self Test Pulse Generation
3. RBC/WBC Current Control
4. CCM to CDM and MPM Data and Control Interface
The voltmeter section of the DCM consists of U3, U7, U6, U10 and associated circuitry. Since the
voltmeter inputs are identical in theory, the Filtered HGB voltage will be used as an example.
The unknown HGB voltage is presented to the input of the comparator at U6-7. The computer then
monitors the comparator output via U3 and uses a successive approximation technique at U6-6 to
read the unknown voltage.
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CIRCUIT DESCRIPTIONS
Self-test pulses are generated, under computer control, by U12. This chip also generates the Current
Select and Current On signals.
Data to and from the CDM is interfaced by PIA (U4) via J2.
Serial Stepper Motor data to and from the MPM is interfaced by ACIA (U2) via J1.
4.8 CABLE DISTRIBUTION MODULE
Refer to Figure 3-3 in Section 3.
The CDM performs the following functions:
1. Status Sensor Interface
2. Control of Solenoid Driver Module
3. Pump Relay Module interface and control
4. Start Board Interface
The CDM communicates with the DCM via the DCM/CDM data bus at J2. Analog outputs of the
Metering Modules are converted to TTL levels by comparators (U12) and placed directly on the DCM/
CDM data bus. Signals from the Pump Relay board, Probe Position Switches, and Start Board are
interfaced by Data Drivers (U5, U10).
Data is interfaced to the Solenoid Driver Modules via J32. This data is then multiplexed by One-ofEight Decoders (U1, U2) via J3, J4, J6, J7, and J9.
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CIRCUIT DESCRIPTIONS
Vacuum and pressure control data is latched by U14 and routed to the Pump Relay Module via J11.
Pump status signals (Vac On, Pres On) are converted to TTL levels by U3 and placed on the data bus
by U5.
LED drive signals are routed to the Start Board via J17. The start signal enters at J17 and is placed
on the data bus by U5.
4.9 SOLENOID DRIVER MODULE (SDM)
Refer to Figure 3-3 in Section 3.
The purpose of the SDM is to provide drive current to the solenoids. Each SDM has eight Darlington
drivers (Q1-Q8) which are individually controlled by data bits (D0-D7) and data latch (U3).
There are two power modes available for each solenoid — activate (+24V) and hold (+12V). This is
controlled by the Hi CLK signal in conjunction with data bits (D0-D7) and the current control latch (U1).
4.10 MOTOR PROCESSOR MODULE (MPM)
Refer to Figure 3-3 in Section 3.
The MPM controls drive data to the Stepper Drive printed circuit boards and also provides self-test
capability for motor winding current. The MPM is comprised of the following major circuits:
1. Microprocessor
2. Program Control E PROM
3. I/O Peripheral Interface Adapter (PIA)
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4. Direct Memory Access (DMA) Control
CIRCUIT DESCRIPTIONS
5. Motor Phase Latches
6. Motor Current Latches
7. Motor Winding Self Test
Control functions of the MPM are performed by microprocessor (U5).
The operating program for the microprocessor is stored in Program Control EPROM (U3).
Data communications between the DCM and MPM are controlled by I/O PIA (U6) and serial data is
interfaced via ACIA (U2) and Data Bus Connector (J1).
Phase data, motor direction, and step rate are stored in RAM (U7). This data is sent to the Motor
Phase Latches under control of the DMA Control circuitry, which consists of U11, U12, U15, U16,
U18, U21 and associated circuitry. The data is strobed into the appropriate Motor Phase Latch by
ALG0 through ALG2.
The Motor Phase Latches U23, U26, and U29 provide phase data to the Stepper Drive printed circuit
boards. Each is an 8-Bit Addressable Latch which can control up to four Stepper Drive printed circuit
boards and subsequently four Stepper Motors.
Four levels of motor current for each motor is controlled by the Motor Current Latches (U22, U25, and
U28). Each latch can control up to four stepper drive printed circuit boards. Data is strobed into the
appropriate latch by WR0 through WR2.
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CIRCUIT DESCRIPTIONS
The Feedback– and Feedback+ inputs at J3 through J14 are connected, via resistors on the Stepper
Drive printed circuit board, to the stepper motor windings. This allows the circuitry consisting of U30,
U31, and U32 to monitor the winding current during an internal self-test. These values can be read by
the CCM to isolate a defective Stepper Drive or Stepper Motor.
4.11 STEPPER DRIVE PRINTED CIRCUIT BOARD
Refer to blocks 20, 23, and 24 off the MPM in Figure 3-3 in Section 3.
The Stepper Drive printed circuit board consists of two PBL 3717 motor drive chips. Each chip drives
a winding of the Stepper Motor. Bits I0 and I1 are used to control four motor current levels:
1. P0 - High Current
2. P1 - Medium Current
3. P2 - Low Current
4. P3 - Current Off
Bits PH0 and PH1 control motor phase and, therefore, direction and step-rate (velocity). Feedback+
and Feedback- are used to generate a motor self-test.
4.12 PRESSURE/VACUUM REGULATOR MODULE
Refer to Figure 3-3 in Section 3 and schematic 9630801.
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CIRCUIT DESCRIPTIONS
Pressure (or vacuum) is sensed by a transducer that is internally configured as a Wheatstone Bridge.
Transistors Q1, R4, and R5 are used to generate a stable reference voltage for the Wheatstone
Bridge. The output of the Wheatstone Bridge is partially amplified (U1-7), stabilized against long term
drift (voltage follower U1-1) and made offset-adjustable by R18 and associated resistors.
Maximum transducer sensitivity can only be achieved when the output is zero volts at TP-1 and when
there is no pressure differential across the transducer. To accomplish this, R18 is adjusted for zero
volts when both transducer inlet ports (P1 and P2) are open to atmospheric pressure.
In order to maintain the operating point of comparator U2-14 at the fixed 2-volt trip level, it is necessary to maintain the output of U2-8 within a relatively narrow range. This is accomplished by making
the differential amplifier (whose inputs are U1-10 and U2-10) adjustable by selecting 1 of 4 possible
jumper positions. A stable reference point for the DC operating level of U1-8 and U2-8 is established
by U1-14 in conjunction with R6 and resistor network RP1.
Measurement of pressure in the range of approximately 0.5 lbs/sq. inch is accomplished by using
transducer inlet port P1 in conjunction with jumper setting A/B.
Vacuum pressure in the range of 8 inch Hg is accomplished by using inlet port P2 in conjunction with
jumper setting C/D. The regulation point for either vacuum or pressure is established by the setting of
potentiometer R16.
When the output of comparator U2-14 goes positive, the collector of Darlington transistor Q2 is pulled
to ground, thereby turning on either the pressure or vacuum pump. When the pumps are running
green, LED DS1 is lit and stays lit until either the pressure or vacuum increases past the hysterisis
point established by R8 of U2-14.
The output of the pressure/vacuum regulator can be inhibited by a logic low at J1-1. This completes
the circuit description.
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Section 4
4.13 LINEAR POWER SUPPLY MODULE
CIRCUIT DESCRIPTIONS
The Linear (Main) Power Supply module generates the following voltages:
1. +12VDC — Cable Distribution Module
2. +24VDC — Cable Distribution Module
3. +100VDC — Pre-Amp Module
4. +15 & -15VDC — Analog Circuit Boards
Refer to Figure 3-5 in Section 3.
4.14 PC POWER SUPPLY MODULE
The PC Power Supply module generates the following voltages:
1. +5VDC — Digital Circuitry and UIC
2. -5VDC — UIC Motherboard
3. +12VDC — Analog Circuitry and UIC
4. -12VDC — Analog Circuitry and UIC
The PC Power Supply also generates a Power Good signal which disables the computer (UIC) in the
event of a power failure or brown-out and prevents good data from being overwritten.
The +5VDC is adjusted by R39. All voltages are fixed.
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Section 4
CIRCUIT DESCRIPTIONS
The voltages generated on the PC Power Supply are routed to their final destinations by the PDM
(Power Distribution Module).
Refer to Figure 3-5 in Section 3.
4.15 24V SWITCHING MODULE
The +24VDC Switching Module regulates power to the Motor Processor Module, via the PDM, to drive
the Stepper Motors. Refer to Figure 3-5.
4.16 PUMP RELAY MODULE (PRM)
Refer to Figure 3-3 in Section 3.
The PRM provides drive to the vacuum and pressure pumps, via three Solid State Relays: K1, K2,
and K3.
4.17 USER INTERFACE COMPUTER (UIC)
The User Interface Computer (UIC) is designed around an Intel-compatible 386SX/DX chip running at
25 — 40 MHz. (Future configuration may be a 486DX chip running at 33 or 66 MHz.) The UIC
receives power from the Switching Power Supply via the Power Distribution Module and receives status and measurement data from the CCM. The board contains EPROM, CMOS RAM, input/output
circuitry for the interface ports, and addressing circuitry.
The following components, described in Section 3 of this manual as part of the User Interface Computer, are purchased OEM from commercial vendors:
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Section 4
1. IDE Controller (for floppy and hard disk drives) and I/O board
CIRCUIT DESCRIPTIONS
2. Input/Output board
3. Super VGA board
4. Computer monitor
No circuit description for these boards is included here. Refer to a standard computer reference manual for more detailed information concerning these boards.
4.18 DATA LINK ADAPTER (DLA)
The Data Link Adapter provides interfaces from the 386/486 computer to both the CCM (Cell Count
Module) and the instrument (membrane) key panel. These two interfaces function independently,
under application software control. However, some of the circuits on the DLA board are shared. The
DLA uses an 82C55 PPI (Parallel Peripheral Interface) IC. Refer to Figure 4-4 for an illustration of the
DLA board.
Interface to CCM
A 20-pin ribbon cable connects the DLA to the CCM. This interface is a bi-directional, parallel interface that is software-controlled at both ends. Data is transferred in 8-bit bytes on 8 data lines (D0 —
D7) in one direction at a time. The UIC (User Interface Computer) initiates an inquiry message handshake on a periodic basis or when it has a command to send. The CCM responds by sending data or
replying to the command sent.
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Section 4
CIRCUIT DESCRIPTIONS
The interface is controlled cooperatively by the UIC and CCM according to the state of the handshake
signals. Refer to Section 4.5, Cell Count Module. The UIC will always send data first; the CCM will
respond by sending data back (if any is available). Thus, the 82C55 switches its A-port from input to
output and back to input during every message transaction.
The key handshake signals are REQ1 and REQ2. REQ2 going low will initiate the communication
protocol. The CCM will respond by bringing REQ1 low. After the DLA has sent its data, it will bring
REQ2 high. Then the CCM will send its data.
Each byte received by the DLA will generate an IRQ. The application software responds to the IRQ
by putting the byte into a buffer. When the CCM brings REQ1 high, the communication is complete;
the DLA will return to an idle state, and port A will be set to input. Both REQ1 and REQ2 will remain
high until the next message/data transfer.
The two LEDs on the printed circuit board, DS1 and DS2, indicate the active state of REQ2 and
REQ1, respectively. They should always be flickering when the application software is running
because the UIC program is constantly polling the CCM to check its state. (There are some exceptions to this during power up and certain diagnostics/test modes.)
The I-O address of the DLA board is assigned by four jumpers. The default I-O address (pins 3 — 6
on S1 hard-wired) is: [off off on off], 340 hexadecimal. The DLA interrupt level is assigned by a
jumper.
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Section 4
Jumpers W1 — W6 assign the DLA interrupt to one of the following:
CIRCUIT DESCRIPTIONS
W1:
IRQ 5 *
W2:I
RQ 10
W3:I
RQ 10
W4:I
RQ 11
W5:I
RQ 12
W6:I
RQ 15
* Since IRQ 5 is used by the DLA, W1 is hard-wired (this is the default).
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CELL-DYN® 1700 Service Manual
CIRCUIT DESCRIPTIONS
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Section 4
Interface to Key Panel
CIRCUIT DESCRIPTIONS
A 14-pin ribbon cable connects the DLA to the key panel. The key panel is also polled by the UIC program. This interface is not interrupt-controlled.
The key panel is interfaced as a parallel switch matrix circuit with 4 lines out and 8 lines in. Four scan
rows are driven active one at a time via an LS175 latch. Then the columns of the key panel matrix are
read from port B of the 82C55. The software interprets a low active signal as a key is pressed.
The DLA board must be plugged into a 16-bit slot. There are no adjustments on the DLA board.
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Section 5
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Section 5.
DIAGNOSTICS AND TROUBLESHOOTING
Table of Contents
5.1 SYSTEM OVERVIEW
5.2 DIAGNOSTICS MENU USAGE
Level One
Level Two
Level Three
Level Four
5.3 FAULT REPORT DESCRIPTION
5.4 CELL-DYN 1700 TROUBLESHOOTING GUIDE
Troubleshooting Chart
Nonfunctional Instrument Problems
Video Display Problems
Displayed Error and Fault Problems
Data Problems
Clog and Flow Error Problems
Miscellaneous Problems
5.5 RAW DATA DESCRIPTION
Raw Data DisplayDescription
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Section 5
5.6 CCM ON-BOARD DIAGNOSTIC LEDS
LED Definition
LED Notation
Examples of Normal Situations
Examples of Bad Situations
Power-on Tests
DIAGNOSTICS AND TROUBLESHOOTING
5.7 CPU HARDWARE/SOFTWARE CONFIGURATION
RS-232 Communications Test Procedure
CMOS Setup
Setup Screen
AMI BIOS
5.8 SERVICE SPECIAL COMMANDSDiscussion
DIAGNOSTICS Menu Service Code Function List
Probe Check
Auto-Cycling (Code 999)
5.9 SAMPLE PROBE DESCRIPTION
Service DEC Codes 128, 129, and 130 Descriptions
Service DEC Code 128
Service DEC Code 129
Service DEC Code 130
Motor Direction Commands
Sample Probe Normal Operation
Initialization Mode
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DIAGNOSTICS AND TROUBLESHOOTING
Stepper Motor Homing
Run Mode
Switch Failure Descriptions
5.10 CELL-DYN 1700 ERROR MESSAGES
Count Test
Operator-Correctable Alarm or Fault Messages
Summary of Error Messages
5.11 SOFTWARE COMMANDS AND SEQUENCE
CD1700 File and Directory Structure
Accessing DOS
CD1700 Program Loaded
CD1700 Program Not Loaded
Exiting DOS
Common DOS Commands
DOS Command Usage
Change Drives
Change Directories/Files Within a Drive
Make a New Directory
Remove a Directory
Compare
Copy
Delete a File
Undelete a File
Rename a File
View Files in a Directory
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Section 5
DIAGNOSTICS AND TROUBLESHOOTING
Software Installation/Upgrades
List of Tables
Table 5-1 Disk Drive Setup Information
Table 5-2 DEC Service Commands
Table 5-3 Motor Power Specifications
Table 5-4 Motor Direction Commands
Table 5-5 Motor Speed Commands
Table 5-6 Error Messages
Table 5-7 Event Messages During Diagnostic Menu Count Test
Table 5-8 Operator-Correctable Alarm or Fault Messages
Table 5-9 Summary of Error Messages
List of Figures
Figure 5-1 Smoothing ON/OFF Example
Figure 5-2 Raw Data Example
Figure 5-3 AMI BIOS
Figure 5-4 Motor Power Test
Figure 5-5 Probe Up/Down Initialize and Run Cycle
Figure 5-6 Probe Initialize Mode
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Section 5
Figure 5-7 Probe Rotate Run Mode
Figure 5-8 Lower Switch (#1) Fault Report
Figure 5-9 Upper Switch (#2) Fault Report
Figure 5-10 Left Switch (#3) Fault Report
Figure 5-11 Right Switch (#4) Fault Report
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Section 5
5.1 SECTION OVERVIEW
DIAGNOSTICS AND TROUBLESHOOTING
This section is designed to aid the service representative in the troubleshooting and repair of the
CELL-DYN 1700/1700CS System. Emphasis is placed on using various System Status and Fault
Reports, which can be accessed by the service representative, to solve problems. Special Service
Commands are also available to exercise and observe mechanical and electronic functions.
5.2 DIAGNOSTICS MENU USAGE
Utilization of the DIAGNOSTICS Menu enables the operator or service representative to identify and
correct both operator-correctable and service-correctable faults. When the computer senses a fault,
the message <NOT READY: SEE DIAGNOSTICS> is displayed in the System Status Box on the
RUN Menu. The following keys are available in the DIAGNOSTICS Menu.
Level One
•
•
•
•
•
INITIALIZATION: Used to perform an Initialization cycle: returns movable components to
"home" position and performs internal self-tests.
RAW DATA: Used to display raw measurement data for the last specimen.
COUNT TEST: Used to run specimens without returning to RUN Menu and display
Raw Data.
MORE: Used to display additional functions.
PRINTER OUTPUT: Used to toggle printer output ON and OFF.
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Section 5
Level Two
•
•
•
•
•
•
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DIAGNOSTICS AND TROUBLESHOOTING
WBC HISTOGRAM: Used to display WBC count and histogram data accumulated in each
of 256 size channels.
RBC HISTOGRAM: Used to display RBC histogram data accumulated in each of 256 size
channels.
PLT HISTOGRAM: Used to display PLT count and histogram data accumulated in each of
256 size channels.
SMOOTHING ON/OFF: Used to toggle histogram display status. With Smoothing Off, only
raw counts are displayed. With Smoothing On, channels are numbered, data is normalized
and the number of the peak channel is shown. Figure 5-1 gives an example of Smoothing
On/Off.
MORE: Used to display additional functions.
PRINTER OUTPUT: Used to toggle printer output ON and OFF.
Level Three
•
•
•
•
PROBE HOME: Moves Sample Probe up and above RBC Cup.
PROBE UP: Moves Sample Probe up.
MORE: Used to display additional functions.
PRINTER OUTPUT: Used to toggle printer output ON and OFF.
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DIAGNOSTICS AND TROUBLESHOOTING
Figure 5-1: Smoothing ON/OFF Example
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Section 5
Level Four
•
•
•
•
•
•
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DIAGNOSTICS AND TROUBLESHOOTING
SYSTEM STATUS: Used to display or print current status.
FAULT REPORT: Used to display or print a fault report.
SERVICE HEX CODES: Not used for operator or service troubleshooting.
SERVICE DEC CODE: Used to initiate individual actions in the CELL-DYN 1700 hardware
and software.
MORE: Used to display additional functions.
PRINTER OUTPUT: Used to toggle printer output ON and OFF.
5.3 FAULT REPORT DESCRIPTION
A detailed list of all faults generated by the CELL-DYN 1700 software and hardware is contained in
Section 5.10. The fault classifications reported in the Fault Report primarily contains data pertaining
to the last CCM fault.
If a fault occurs, pressing the [HELP/ERROR] key will immediately display the Fault Log in the DIAGNOSTICS Menu. This log may contain up to 16 faults, with the current fault leading the list. An alternative procedure is to go to the MAIN MENU and press [DIAGNOSTICS]. In this case, the Fault
Report, not the Fault Log, is immediately displayed.
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DIAGNOSTICS AND TROUBLESHOOTING
Section 5
To view the Fault Log when no fault is pending, from the MAIN MENU press [DIAGNOSTICS] followed by [HELP/ERROR] and [HELP]. The system will display up to 16 past faults. Press [FAULT
REPORT] to display the FAULT REPORT screen. A display of <NO FAULTS OR WARNINGS
PENDING> indicates that all faults have been cleared.
5.4 CELL-DYN 1700 TROUBLESHOOTING GUIDE
A list of symptoms, probable causes, and corrective actions for the most common problems encountered on the CELL-DYN 1700 is given in the Troubleshooting Chart below. The probable causes and
corrective actions are arranged in descending order from most likely to least likely. When troubleshooting a problem, start with the most likely cause first.
If possible, thoroughly verify that a component is defective before replacement. Some problems can
be verified visually, but other problems require a DVM (Digital Volt Meter).
When troubleshooting "DATA PROBLEMS", only the measured parameters RBC, PLT, WBC, HGB,
and MCV should be used for reference. Using the calculated parameters can become confusing
when trying to isolate a problem.
When troubleshooting "CLOG AND FLOW ERROR PROBLEMS", refer to Figure 8-1 in Section 8 for
the "MIN" and "MAX" specifications for the RBC and WBC Upper (T1) and Lower (T2) times.
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Section 5
Troubleshooting Chart
DIAGNOSTICS AND TROUBLESHOOTING
Nonfunctional Instrument Problems
SYMPTOM
PROB. CAUSE
CORRECTIVE ACTION
1. NO FUNCTS.
NO FANS
1. FUSE
1. CHECK FUSE
2. POWER CORD
1. CHECK POWER CORD
3. POWER.
SOURCE
1. CHECK POWER
SOURCE
1. NO 5VDC
1. CHECK PC POWER
SUPPLY
2. VDM SHORT.
A.C. POWER
1. REPLACE VDM
SYMPTOM
PROB. CAUSE
CORRECTIVE ACTION
1. CRT BLANK
SOLENOIDS OK
1. BRIGHT. CONT.
1. INCREASE
BRIGHTNESS
2. DEFECT. VDM
1. REPLACE VDM
1. HORIZ. CONT.
1. ADJUST FRONT LINE ON CRT
CONTROLS
2. NO FUNCTS.
FANS RUN
Video Display Problems
2. HORIZONTAL
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Section 5
SYMPTOM
3. VERTICAL
LINE ON CRT
4. DOT IN CNTR.
OF CRT
5. ROLLING
IN VERT.
6. NONLINEAR
7. INCORRECT
8. INCORRECT
HORIZ. WIDTH
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DIAGNOSTICS AND TROUBLESHOOTING
PROB. CAUSE
CORRECTIVE ACTION
2. DEFECT. VDM
1. REPLACE VDM
1. VERT. CONT.
1. ADJUST FRONT
CONTROLS
2. DEFECT. VDM
1. REPLACE VDM
1. MISADJUST.
1. ADJUST FRONT
CONTROLS
2. DEFECT. VDM
1. REPLACE VDM
1. MISADJUST.
1. ADJUST FRONT
CONTROLS
2. DEFECT. VDM
1. REPLACE VDM
1. MISADJUST.
IN VERT.
1. ADJUST FRONT
CONTROLS
2. DEFECT. VDM
1. REPLACE VDM
1. MISADJUST.
1. ADJUST FRONT VERTICAL SIZE
CONTROLS
2. DEFECT. VDM
1. REPLACE VDM
1. MISADJUST.
1. ADJUST FRONT
CONTROLS
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Section 5
SYMPTOM
9. CHARACTERS
OUT OF FOCUS
10. CHARACT. OK
BUT GARBLED
11. MISSING
CHARACTERS
12. MISS HORIZ
OR VERT LINES
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DIAGNOSTICS AND TROUBLESHOOTING
PROB. CAUSE
CORRECTIVE ACTION
2. DEFECT. VDM
1. REPLACE VDM
1. MISADJUST.
1. ADJUST FRONT
CONTROLS
2. DEFECT. VDM
1. REPLACE VDM
1. VIDEO CABLE
1. CHECK VIDEO CABLE
2. DEFECT. SVGA
1. REPLACE SVGA BOARD
3. DEFECT. VDM
1. REPLACE VDM
4. DEFECT. UIC
1. REPLACE UIC
1. VIDEO CABLE
1. CHECK VIDEO CABLE
2. DEFECT. SVGA
1. REPLACE SVGA BOARD
3. DEFECT. VDM
1. REPLACE VDM
4. DEFECT. UIC
1. REPLACE UIC
1. VIDEO CABLE
1. CHECK VIDEO CABLE
2. DEFECT. SVGA
1. REPLACE SVGA BOARD
3. DEFECT. VDM
1. REPLACE VDM
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Section 5
SYMPTOM
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DIAGNOSTICS AND TROUBLESHOOTING
PROB. CAUSE
CORRECTIVE ACTION
4. DEFECT. UIC
1. REPLACE UIC
Displayed Error and Fault Problems
SYMPTOM
PROB. CAUSE
CORRECTIVE ACTION
1. DET EMPTY
1. RESTRICTION
1. CHECK LINES
2. CHECK SOL 4-1
2. DEFECTIVE
SENSOR
1. CHECK SENSOR
2. REPLACE SENSOR
2. DIL. EMPTY
3. DEFECT. CDM
1. REPLACE CDM
1. SOL 3-1
TUBING
1. CHECK SOL 3-1
2. RESTRICTION
1. CHECK LINES
2. CHECK SOL 4-2
3. DEFECT.
SENSOR
1. CHECK SENSOR
2. REPLACE SENSOR
4. DEFECT. CDM
1. REPLACE CDM
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Section 5
SYMPTOM
3. PRESSURE
OVERLIMIT
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DIAGNOSTICS AND TROUBLESHOOTING
PROB. CAUSE
CORRECTIVE ACTION
1. SOLENOID 3-3
1. CHECK SOL 3-3
2. REPLACE SOL 3-3
2. SOLENOID 3-4
1. CHECK SOL 3-4
2. REPLACE SOL 3-4
3. SOLENOID 3-1
1. CHECK SOL 3-1
2. REPLACE SOL 3-1
4. DEFECTIVE
SWITCH
4. VAC LO ERR
5. PRES LO ERR
1. CHECK SWITCH
2. REPLACE SWITCH
5. DEFECT. CDM
1. REPLACE CDM
1. LEAK 8" HG
1. CHECK PLUMBING
2. DEFECT. PUMP
1. REPLACE PUMP
3. DEFECT.
VAC REG
1. REPLACE REG
1. LEAK 0.6 PSI
1. CHECK PLUMBING
2. DEFECT. PUMP
1. REPLACE PUMP
3. DEFECT.
PRES REG
1. REPLACE REG
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Section 5
SYMPTOM
6. WASTE OVRFL
INTO ACCUM
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DIAGNOSTICS AND TROUBLESHOOTING
PROB. CAUSE
CORRECTIVE ACTION
1. NO AIR PRES.
1. CHECK PRESSURE
2. REPLACE PUMP
3. REPLACE CDM
2. SOL 5-3 STUCK
1. CHECK SOL 5-3
2. REPLACE S0L 5-3
3. SOL 5-7 STUCK
1. CHECK SOL 5-7
2. REPLACE SOL 5-7
4. SENSOR NOT
DETECTING
1. CHECK SENSOR
2. REPLACE SENSOR
3. REPLACE CDM
7. WASTE EMPTY
TIMEOUT
1. DEFECTIVE
SENSOR
1. CHECK SENSOR
2. REPLACE SENSOR
2. AIR PRESSURE
LOW
1. CHECK PRESSURE
2. REPLACE PUMP
3. RESTRICTION
1. CHECK PLUMBING
4. DEFECT. CDM
1. REPLACE CDM
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Section 5
SYMPTOM
8. CCM/UIC
9. DISK ERRORS
10. POSITION
FAULTS
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PROB. CAUSE
1. UIC/CCM CABLE
DISCON.
DIAGNOSTICS AND TROUBLESHOOTING
CORRECTIVE ACTION
1. CHECK UIC/CCM
CABLE
2. DEFECT.
UIC/CCM CABLE
1. REPLACE UIC/CCM
CABLE
3. DEFECT. CCM
BOARD
1. REPLACE CCM BOARD
4. DEFECT. DLA
BOARD
1. REPLACE DLA BOARD
5. DEFECT. UIC
1. REPLACE UIC
1. DEFECT. UIC
1. CHECK CMOS SETUP
2. CHECK UIC/CCM
CABLE
3. REPLACE UIC
2. DEFECT. DISK
DRIVE
1. REPLACE DISK DRIVE
1. MISALIGN
SWITCH
1. PERFORM
ALIGNMENT
2. DEFECT.
SWITCH
1. REPLACE SWITCH
AND PERFORM ALIGNMNT
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Section 5
SYMPTOM
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DIAGNOSTICS AND TROUBLESHOOTING
PROB. CAUSE
CORRECTIVE ACTION
3. DEFECT. CDM
1. REPLACE CDM
4. DEFECT.
1. RUN MOTOR POWER
DRIVE PRINTED
CIRCUIT BOARD
2. REPLACE DRIVE PRINTED
CIRCUIT BOARD
5. DEFECT.
SAMPLE PROBE
ASSEMBLY
1. EXERCISE PROBE
TEST
2. REPLACE ASSEMBLY
6. DEFECT.
MOTOR
1. RUN MOTOR POWER R
TEST
2. REPLACE MOTOR
SYMPTOM
PROB. CAUSE
CORRECTIVE ACTION
1. ALL RESULTS
ARE "0"
1. NO +/- 15VDC
1. CHECK +/- 15VDC
2. REPLACE PSM
2. HGB OK ALL
OTHERS "0"
1. NO 100VDC
1. REPLACE PSM
2. DEFECT. SPM
1. REPLACE SPM
Data Problems
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Section 5
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DIAGNOSTICS AND TROUBLESHOOTING
3. DEFECT. PAM
1. REPLACE PAM
SYMPTOM
PROB. CAUSE
CORRECTIVE ACTION
3. HGB "0" ALL
OTHERS OK
1. NO REF.
ASPIRATION
1. CHECK PLUMBING
2. NO SAMPLE
ASPIRATION
1. CHECK PLUMBING
3. DEFECT. PAM
1. CHECK PAM TP2
2. REPLACE PAM
4. DEFECT. DCM
1. CHECK DCM TP5
2. REPLACE DCM
5. DEFECTIVE
FLOW CELL
1. REPLACE FLOW
CELL
1. BUBBLE MIX
INCORRECT
1. CHECK BUBBLE MIX
PRESSURE
2. CHECK PLUMB.
2. INCORRECT
PROBE HEIGHT
1. CHECK PROBE
HEIGHT
2. ADJUST PROBE HEIGHT
3. INADEQUATE
PROBE CLEANING
1. CHECK PLUMBING
4. ERRATIC DATA
ALL PARAMS
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Section 5
SYMPTOM
5. ERRATIC DATA
HGB OK
6. ERRATIC RBC
& PLT, WBC OK
7. ERRATIC WBC
RBC AND PLT OK
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DIAGNOSTICS AND TROUBLESHOOTING
PROB. CAUSE
CORRECTIVE ACTION
4. IMPRECISE
SAMPLE
ASPIRATION
1. CHECK SAMPLE
SYRINGE
2. CHECK PLUMBING
5. IMPRECISE
DILUENT DISP.
1. CHECK DILUENT
SYRINGE
2. CHECK PLUMBING
1. DEFECT. SPM
1. REPLACE SPM
2. DEFECT. PAM
1. REPLACE PAM
1. DIRTY TRANS.
1. CLEAN TRANSDUCER
2. INCORRECT
BUBBLE MIX
1. CHECK RBC
PLUMBING
3. DEFECT. SPM
1. REPLACE SPM
1. DIRTY TRANS.
1. CLEAN TRANSDUCER
2. INCORRECT
1. CHECK WBC BUBBLE MIX
PLUMBING
3. DEFECT. SPM
1. REPLACE SPM
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Section 5
SYMPTOM
8. ERRATIC HGB
OTHERS OK
9. ERRATIC MCV
AND HCT
10. WBC "R"
CODE, REAGNTS.
OK
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DIAGNOSTICS AND TROUBLESHOOTING
PROB. CAUSE
CORRECTIVE ACTION
1. DIRTY FLOW
CELL
1. CLEAN FLOW
CELL
2. AIR LEAK
1. CHECK PLUMBING
3. DEFECTIVE
FLOW CELL
1. REPLACE FLOW
CELL
1. DIRTY TRANS.
1. CLEAN TRANSDUCER
2. DIL. BRIDGE
1. SHIM RBC CUP
3. DEFECT. SPM
1. REPLACE SPM
1. DIRTY TRANS.
1. CLEAN TRANSDUCER
2. INCORRECT
LYSE VOLUME
1. CHECK VOLUME
2. ADJUST VOLUME
3. INCORRECT
BUBBLE MIX
1. CHECK BUBBLE
MIX PRESSURE
2. CHECK PLUMBING
4. SLOW TRANS.
TO WBC CUP
1. CHECK PLUMB.
5. INCORRECT
GAIN
1. CHECK GAIN
2. ADJUST GAIN
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SYMPTOM
11. HI BKGNDS,
REAGENTS OK
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DIAGNOSTICS AND TROUBLESHOOTING
PROB. CAUSE
CORRECTIVE ACTION
6. DEFECT. SPM
1. REPLACE SPM
1. "DIRTY" PWR
1. CHECK POWER
2. ISOLATE LINE
3. INSTALL FILTER
2. POOR
GROUNDING
1. CHECK GROUNDING
2. INSTALL GROUND
12. HI BKGNDS
WBC ONLY
REAGENTS OK
3. "NOISY" PSM
1. CHECK PSM
2. REPLACE PSM
4. DEFECT. PAM
1. REPLACE PAM
1. INCORRECT
BUBBLE MIX
1. CHECK BUBBLE
MIX PRESSURE
2. ADJUST PRESSURE
2. SPM
1. INSTALL
Clog and Flow Error Problems
SYMPTOM
PROB. CAUSE
CORRECTIVE ACTION
1. "CLOG"
BOTH SIDES
CONSTANT
1. TRANSDCRS
REVERSED
1. CHECK TRANSDUCERS
2. REINSTALL
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DIAGNOSTICS AND TROUBLESHOOTING
2. INCORRECT
VACUUM
1. CHECK VACUUM
2. ADJUST VACUUM
3. DILUENT AS
DETERGENT
1. CHECK REAG LINES
2. "CLOG"
T1=MAX T2=0,
NO VENT
1. RESTRICTION
1. CHECK VENT PLUMB.
3. "CLOG"
T1=MAX T2=0,
NO MENISCUS
1. DIRTY TRANS.
1. CLEAN TRANSDUCER
2. RESTRICTION
1. CHECK PLUMBING
2. CHECK COUNT SOL.
3. CHECK VENT SOL.
4. CHECK APERTURE
4. "CLOG"
T1=MAX T2=0
SLOW MENISCUS
1. DIRTY TRANS.
1. CLEAN TRANSDUCER
2. RESTRICTION
1. CHECK PLUMBING
2. CHECK COUNT SOL.
3. CHECK VENT SOL.
4. CHECK APERTURE
3. INCORRECT
VACUUM
1. CHECK VACUUM
2. ADJUST VACUUM
2. CHECK VENT SOL.
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DIAGNOSTICS AND TROUBLESHOOTING
Section 5
5. "CLOG"
1. DEF UPPER
1. CHECK UPPER T2=0
T1=MAX
DET
DET
MENISCUS SPEED
2. REPLACE MET PRINTED
OK
CIRCUIT BOARD
2. DEFECT. CDM
6. "CLOG"
1. DEF LOWER
T1=OK
DET
T2=MAX
MENISCUS SPEED OKCIRCUIT BOARD
7. "FLOW ERR"
T1=MIN
T2=MAX
8. "FLOW ERR"
T1=OK
T2=MIN
1. REPLACE CDM
1. CHECK LOWER
DET
2. REPLACE MET PRINTED
2. DEFECT. CDM
1. REPLACE CDM
1. DEF UPPER
DET
1. CHECK UPPER
DET
2. REPLACE MET PRINTED
CIRCUIT BOARD
2. DEFECT. CDM
1. REPLACE CDM
1. DEF LOWER
DET
1. CHECK LOWER
DET
2. REPLACE MET PRINTED
CIRCUIT BOARD
2. DEFECT. CDM
1. REPLACE CDM
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Section 5
Miscellaneous Problems
DIAGNOSTICS AND TROUBLESHOOTING
SYMPTOM
PROB. CAUSE
CORRECTIVE ACTION
1. GARBLED
HISTOGRAMS
1. DEFECT. SPM
1. REPLACE SPM
2. DEFECT. CCM
1. REPLACE CCM
5.5 RAW DATA DESCRIPTION
From the MAIN MENU, press [DIAGNOSTICS] followed by [RAW DATA]. The [RAW DATA] key will
display raw data obtained from the last count cycle.
When a single count is done, all data is contained in the first column. When a PLT recount occurs,
data from the first cycle appears in column #2 and data from the recount appears in column #1.
Raw Data Display Description
RBC, WBC and PLT counts are RAW, uncorrected total counts.
HGB Error is not used.
HGB Reference is the output of the A/D Converter when reading Reference (2000 = 5 volts).
HGB Sample is the output of the A/D Converter when reading Sample (2000 = 5 volts).
WBC and RBC Up Times are the upper times in milliseconds for the last sample.
WBC and RBC Count Times are the times in milliseconds for the last sample.
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Section 5
Flow Error is coded Clog or Flow Error data.
DIAGNOSTICS AND TROUBLESHOOTING
RBC RER is RBC Cell Editing percentage.
WBC and RBC Upper max and Upper min are the maximum and minimum Upper Times, respectively.
WBC and RBC Avg. Time are the averages of the previous count times.
WBC and RBC Time-Outs are the floating Upper Clog Alarm Limits calculated by the "Running Average Program".
An example of a raw data report is shown in Figure 5-2.
5.6 CCM ON-BOARD DIAGNOSTIC LEDS
The seven LEDs on the CELL-DYN 1700 CCM can reveal much about the fundamental CCM and
overall machine state. In general, the LEDs indicate whether the CCM is in a normal functioning
mode or in a fault state, and in either case, help to characterize the CCM state. Also, one of the LEDs
gives some information about the state of the UIC as well.
The CCM tests itself on power-up. These fundamental tests include ROM, RAM, and VIA. If any test
fails, the CCM will attempt to execute a routine which will flash the green LED on the board. Also, it
will place a 4-bit fault code into the adjacent yellow LEDs.
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DIAGNOSTICS AND TROUBLESHOOTING
Figure 5-2: Raw Data Example
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Section 5
LED Definition
Lowest
DIAGNOSTICS AND TROUBLESHOOTING
Highest
DS1 DS2
DS3
DS4 DS5
DS6
DS7
DS1 (green) program controlled, used for CCM go/no go board status.
DS2 through DS5, program controlled, general use is for cell count status; on power up, used for fault
codes.
DS6 program controlled, indicates CCM has requested to send a message.
DS7 controlled by UIC, indicates UIC has requested to send a message.
LED Notation
g
gs
y
yf
ys
=
=
=
=
=
=
LED is off
green LED on, not flashing
green LED flashing slowly (approx. 1 Hz)
yellow LED on, not flashing
yellow LED fast-flickering
yellow LED flashing at slow, non-periodic rate
Examples of Normal Situations
LED Pattern Description
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DIAGNOSTICS AND TROUBLESHOOTING
Section 5
g - - - - yf yf Typical operational state. Green LED is on. Right-most two yellow LEDs are
flashing at fast-flicker, showing UIC/CCM communications. Pattern when
machine is idle (even in STANDBY).
g - - - - ys ys Active operational state. Green LED is on. Rightmost two yellow LEDs are
flashing at slowflicker, showing UIC/CCM communication slowed while CCM
is busy with some process.
g y - - - ys ys Active operational state. RBC cells are being counted.
g - y - - ys ys Active operational state. WBC cells are being counted.
g------
If in either state for no more than approximately thirty seconds, then UIC is
busy, most likely with disk access, e.g., loading a program. State of CCM not
apparent.
Examples of Bad Situations
LED Pattern Description / Probable Cause
-------
CCM is non-functional. UIC is also non-functional or time-out. (Loss of +5V
power?)
g-----y
CCM failed; is non-functional. UIC is attempting to communicate.
g y y y y - y CCM failed; is non-functional.
or
gyyyy--
Most likely got a partial reset which reset the VIA.
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Section 5
g------
g----y-
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DIAGNOSTICS AND TROUBLESHOOTING
If in this state for more than thirty seconds, then UIC is non-functional; it has
failed or has timed-out. If display indicates time-out, then suspect that the
CCM failed, and its failure led to UIC Time-out.
If in this state for more than thirty seconds, and if display indicates time-out,
then most likely CCM failed, and its failure led to UIC Time-out. CCM was
busy at the time of failure.
Power-on Tests
1.
gs - - - y - y CCM failed ROM test, on 1st checksum byte.
2.
gs - - y - - y CCM failed ROM test, on 2nd checksum byte.
3.
gs - - y y - y CCM failed VIA test, register checked
(DDRA).
4.
gs - y - - - y CCM failed VIA test, register checked (IER).
5.
gs - y - y - y CCM failed VIA test, register checked (IFR).
6.
gs - y y - - y CCM failed VIA test, register checked (VCR).
7.
gs - y y y - y CCM failed VIA test, register checked (DDRB).
8.
gs y - - - - y CCM failed RAM test, walking 1's.
9.
gs y - - y - y CCM failed RAM test, on clearing to zero.
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Section 5
5.7 CPU HARDWARE/SOFTWARE CONFIGURATION
DIAGNOSTICS AND TROUBLESHOOTING
RS-232 Communications Test Procedure
Detailed information on the CELL-DYN 1700 RS-232 specifications is contained in Appendix C of this
service manual. For testing the COM1 port, it is recommended that a commercial program, such as
QAPlus© or CHECK(IT PRO, be used.
CMOS Setup
The CMOS Setup contains all the information needed by the BIOS system to establish proper communications between the motherboard and the various computer system devices. The configuration
for the current motherboard is listed below:
Current motherboard
IDE hard drive
IDE controller
CELL-DYN® 1700 Service Manual
Part No.
1700019
2005712
1700021
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Section 5
The disk drive setup information is listed in Table 5-1 below
Table 5-1:
Hard Disk
Drive C
Floppy
Disk Drive
3.5”
DIAGNOSTICS AND TROUBLESHOOTING
Disk Drive Setup Information
Type Cycl Head
WP
47
LZ
Sect
Size
(MB)
944
14
65535 65535
40
258
NA
NA
NA
NA
1.44
NA
Setup Screen
The Setup screen is the user interface to the Basic Input-Output System (BIOS) which resides on the
battery backed-up CMOS RAM chip on the motherboard. The Setup screen is used to input the hardware configurations of the various devices, so that the BIOS can set up proper communications within
the computer.
The BIOS currently shipped on the CELL-DYN 1700 was developed by American Megatrends Inc.
(AMI). The AMI BIOS setup is illustrated in Figure 5-3.
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Section 5
AMI BIOS
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DIAGNOSTICS AND TROUBLESHOOTING
The only way to gain access to the AMI BIOS setup (in DOS) is through the “Boot” cycle. A message
is displayed at the beginning of the Boot cycle that prompts the user to press the Delete key to enter
the Setup screen. Pressing the Delete key during the “time window” allowed by the software is the
only requirement for entering the Setup screen. (Refer to Section 5.11.)
NOTE: At any C prompt, simultaneously pressing the Control, Alt and Delete keys will re-boot the
system and provide an opportunity to enter the Setup screen.
5.8 SERVICE SPECIAL COMMANDS
Discussion
Several commands are available to initiate individual actions in the CELL-DYN 1700 hardware and
software. These commands are used for troubleshooting and/or alignment when a single action is
desired or required to be repeated several times.
The special command mode resides in the DIAGNOSTICS Menu. From the MAIN Menu, press
[DIAGNOSTICS] followed by [SERVICE DEC CODE]. When this softkey is pressed, the message
<SERVICE FUNCTION ONLY: ENTER COMMAND:> is displayed.
A command can now be entered. Pressing the Enter key on the keyboard will initiate the action. Only
one command can be entered at a time and [SERVICE DEC CODE] must be pressed before a command is entered.
All commands available by direct softkey can be accessed by pressing [MORE].
NOTE: Use only the commands listed in Table 5-2 and always verify that the correct number has been
entered before initiating the action. Use only those numbers listed below. Other numbers may
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DIAGNOSTICS AND TROUBLESHOOTING
Section 5
refer to engineering commands which are not to be used in the field and which may cause damage if used improperly. Be fully aware of the purpose of any of the following commands before
using them. This is a direct-activation method which should be used with caution because the
physical state of the CELL-DYN 1700 may not be in agreement with the function to be performed. After using service commands, always re-initialize the system by turning the power
OFF then ON again or by pressing the [INITIALIZATION] key in the DIAGNOSTICS Menu to
ensure the instrument is in the proper configuration for normal operations.
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DIAGNOSTICS AND TROUBLESHOOTING
Figure 5-3: AMI BIOS
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DIAGNOSTICS AND TROUBLESHOOTING
Figure 5-3: (continued)
NOTE: Enable Daylight Savings only in the U.S.
Under Advanced CMOS Setup, pressing the F6 key displays the default settings. The settings
displayed in Figure 5-3 above are set at default except for the bolded settings, which have been
changed for the CELL-DYN 1700.
The normal system bootup sequence is drive C:, A:. During instrument service, if it is necessary to boot from the floppy disk, change the bootup sequence to A:, C:. When service is complete, the sequence must be changed back to C:, A:.
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Section 5
DIAGNOSTICS Menu Service Code Function List
DIAGNOSTICS AND TROUBLESHOOTING
When the [SERVICE DEC CODE] key is pressed, the following prompt is shown:
Enter number (currently, 102):____
The number above corresponds to the decimal code for the last code entered.
Table 5-2 lists the decimal-coded (DEC) service commands that can be invoked by pressing the
[SERVICE DEC CODE] key in the DIAGNOSTICS Menu and entering the appropriate number.
Table 5-2:
DEC Service Commands
UIC
DEC
Codes
07
08
09
11
15
16
17
18
19
Function
not used
not used
not used
not used
fill lyse into system
not used
not used
not used
fill saline & detergent (non-cs)
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Table 5-2:
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UIC
DEC
Codes
20
22
23
24
25
26
33
34
36
37
38
39
40
41
47
48
DIAGNOSTICS AND TROUBLESHOOTING
DEC Service Commands (Continued)
Function
mini-wash
not used
prime (cs)
fill saline & detergent (cs)
daily shutdown (cs)
clean for shipping (cs)
short sample sensor setup
short sample sensor restore
clean close sampler (cs)
pre-dilute sample run setup
pre-dilute sample run exit
aperture current off (uses whole blood script)
open all valves
closed sample run
platelet recount
initialization (homing)
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Section 5
Table 5-2:
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UIC
DEC
Codes
49
50
51
52
53
54
55
56
57
59
60
61
62
63
64
65
DIAGNOSTICS AND TROUBLESHOOTING
DEC Service Commands (Continued)
Function
open sample run
clean orifice (back-flushing)
pre-dilute sample run
background count run (1)
prime system with all reagent
daily shutdown (non-cs)
empty transducers and cups
gain adjust
unpinching normally closed valves
fill transducers and cups after empty
gain adjustment setup
dispense 10 ml saline
open sample wash
clean-for-shipping (non-cs)
clean sample syringe setup
aspirate 40 µl sample for 1/250 dilution
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Section 5
Table 5-2:
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UIC
DEC
Codes
66
67
68
69
71
72
73
74
75
76
77
78
81
83
84
85
DIAGNOSTICS AND TROUBLESHOOTING
DEC Service Commands (Continued)
Function
dispense 10 ml saline for 1/250 dilution
aspirate 100 µl sample for 1/50 dilution
dispense 5 ml for 1/50 dilution
closed sample wash
lyse syringe down
move probe for blood transfer cup adjustment
restore probe after blood transfer cup adjustment
lyse syringe up and home
lyse syringe down restore
pre-dilute sample wash
not used
not used
enzyme clean the system (cs)
diluent syringe down
enzyme clean setup
probe up and rotate and home
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Section 5
Table 5-2:
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UIC
DEC
Codes
86
87
88
89
90
91
92
93
117
118
119
120
121
122
123
DIAGNOSTICS AND TROUBLESHOOTING
DEC Service Commands (Continued)
Function
back to ready position from probe home
probe up for probe adjustment
probe down (when finished, operator should initialize the instrument to place the probe in the
home position)
sample syringe up and restore
sample syringe down and home
enzyme clean the system (non-cs)
diluent syringe up and home
diluent syringe down and restore
not used
not used
not used
not used
cycle solenoids on waste assy
cycle solenoids on flow panel assy
sample syringe aspirate
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Section 5
Table 5-2:
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UIC
DEC
Codes
124
125
126
127
DIAGNOSTICS AND TROUBLESHOOTING
DEC Service Commands (Continued)
Function
sample syringe dispense
vacuum test
check mixing pressure
check backflush pump
relieve pressure; stop reagent syringe (2)
V_IDLE - standard flow script end (3)
rotate probe from home to pre-mix cup (3)
prime the system (non-cs) (3)
prime the system (cs) (3)
clean aperture (3)
clean transducers and cups (3)
fill reagents into transducers and cups (3)
lyse into system (3)
probe up subscript (3)
probe down subscript (3)
wash probe down (3)
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Table 5-2:
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DIAGNOSTICS AND TROUBLESHOOTING
DEC Service Commands (Continued)
UIC
DEC
Codes
Function
aspirate 10 mL diluent (3)
(1) Uses same flow script as open sample run
(2) Not a sub-script, no number assigned
(3) Subscript, no number assigned
NOTE: Certain commands are not sent to the CCM when the system is in an interlock state, such as
STANDBY or UNINITIALIZED.
The following are descriptions of some of the special complex functions:
Probe Check
There are two probe check functions activated by softkeys. One moves the probe up and down without rotational motion, the other homes the probe as in the Initialization procedure.
NOTE: Neither procedure puts the probe in the STANDBY position (on the left). Note also that the Up/
Down Probe Check procedure leaves the probe assembly free to move back and forth. The
cursor now hovers over the appropriate key when the user is carrying out a multiprocess sequence (such as Probe Up/Down).
Auto-Cycling (Code 999)
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DIAGNOSTICS AND TROUBLESHOOTING
Section 5
The CELL-DYN 1700 can be pre-set to do a specified number of RUN cycles without user intervention. This capability applies only to normal RUN Count Test, Pre-Dilute RUN, (PRE-DIL TEST), Gain
Adjust (GAIN ADJ), and Electrical Background (ELEC BKGD). This capability will help reduce test
time for the instrument. The following entry screen will appear after entering code 999:
-- Auto Cycle Test Set Up -Use "#" key to accept current number
Use "<-" key to delete a digit
Use "*" key to cancel
Enter Number of Times to Repeat Test (currently, 10):1
Auto Cycling ON
5.9 SAMPLE PROBE DESCRIPTION
The motors that enable the Sample Probe to move up/down and to rotate are stepper motors which
are under direct computer control. Since there is no direct positional feedback sent to the computer,
position switches are employed to verify critical positions during normal operation. It is important to
understand that these switches only verify and do not control the movement of the Sample Probe.
In the DIAGNOSTICS Menu, Service DEC Codes 128, 129, and 130 allow the service representative
to control and exercise all stepper motors in the CELL-DYN 1700. This description will focus on the
Probe Up/Down Motor (B/2) and the Probe Rotate Motor (C/3) which control the movement of the
Sample Probe.
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DIAGNOSTICS AND TROUBLESHOOTING
Section 5
The procedures for aligning the Position Switches and aligning the Sample Probe height are
described Section 8. In order to better understand these procedures, read the following descriptions
of the normal operation of the Sample Probe, descriptions of switch failures, and a description of Service DEC Codes 128, 129, and 130.
Service DEC Codes 128, 129, and 130 Descriptions
These commands reside in the SERVICE DEC CODE screen of the DIAGNOSTICS Menu and are
used to test, control, and exercise CELL-DYN 1700 stepper motors. A description of each of these
three commands is given below.
Service DEC Code 128
This code runs a computer generated test (Motor Power Test) of all stepper motors, motor driver
boards, and associated circuitry.
The Motor Power Test should be run whenever a problem is suspected with any assembly that is
driven by a stepper motor. The test should also be run before performing any Sample Probe alignment procedure.
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DIAGNOSTICS AND TROUBLESHOOTING
Section 5
The following entry screen will appear after entering code 128:
Motor Power Test Started.
To MPM: {I }
To MPM: {pD32}
To MPM: {mC1!2 } AC}
To MPM: {C1 }
inp: 0415
A report is automatically displayed and can be printed. An example of the Motor Power Test is shown
in Figure 5-4. When the results of this test are displayed, compare the data shown for the motor being
tested to the specifications shown in Table 5-3, Motor Power Specifications.
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DIAGNOSTICS AND TROUBLESHOOTING
Section 5
Press the [INITIALIZE] key before leaving the DIAGNOSTICS Menu.
Figure 5-4: Motor Power Test
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Table 5-3:
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DIAGNOSTICS AND TROUBLESHOOTING
Motor Power Specifications
MOTOR
A,D
B,H
C,E
NOMINAL LOW
2.0 (+/- .4)
1.7 (+/- .34)
2.3 (+/- .46)
NOMINAL MED.
4.3 (+/- .86)
3.7 (+/- .74)
6.5 (+/- 1.3)
NOMINAL HIGH
6.1 (+/- 1.22)
5.2 (+/- 1.04)
9.8 (+/- 1.96)
NOMINAL PHASE
6.1 (+/- 1.22)
5.2 (+/- 1.04)
9.8 (+/- 1.96)
The tolerances for the values are +/- 20% of the nominal value.
Service DEC Code 129
This code allows the "Run" and "Idle" power levels to be set when exercising a stepper motor. The
four levels are:
0) Full Power
1) Medium Power
2) Low Power
3) Off
This code tests mechanical assemblies at various power levels or to remove idle power so the mechanism can be more easily moved or checked manually. The following entry screen will appear after
entering code 129 (press the ENTER key after each entry):
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DIAGNOSTICS AND TROUBLESHOOTING
-- Testing Motor Power Levels -Use "#" key to accept current number
Use "<-" key to delete a digit
Use "*" key to cancel
Motor Letters: A B C D E F G H I J K L
Number:
1 2 3 4 5 6 7 8 9 10 11 12
Enter Corresponding Motor Number (1 to 12, currently 1): 1
Enter Running Power (0 - max to 3 - off, currently 1):1
Enter Idle Power (0 - max to 3 - off, currently 1): 3
After the entries have been made, a message will appear such as:
Motor "A" set to running power of 1 and idle power of 3
Service DEC Code 130
This code allows the direction, speed, and number of steps to be set when exercising a stepper motor.
The following entry screen will appear after entering code 130:
-- Motion Check Testing -Use "#" key to accept current number
Use "<-" key to delete a digit
Use "*" key to cancel
Motor Letters: A B C D E F G H I J K L
Number:
1 2 3 4 5 6 7 8 9 10 1112
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Section 5
Enter Corresponding Motor Number (1 to 12, currently 2): 10
Enter Direction (0 or 1, currently 0):1
Spd Code:
Number:
DIAGNOSTICS AND TROUBLESHOOTING
! " # $ % & ' ( ) * + , - . / 0 1
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17
Enter Corresponding Speed Number (1 to 17, currently 6): 6
Enter Number of Steps (1 to 999, currently, 100): 100
After the entries have been made, a message will appear such as:
Motor "B" : motion in direction “0” at speed “&” for 100 steps
Motor Direction Commands
Table 5-4 contains information on the motor designation, command and direction of the motor to be
tested. Table 5-5 lists the motor speed commands to determine the speed of the motor being tested.
Both tables are needed to properly test the motor.
Table 5-4:
Motor Direction Commands
Motor
Designations
Function
A/1
Sample Syringe
B/2
Probe Up/Down
CELL-DYN® 1700 Service Manual
Command
0
1
0
1
Direction
Down/Aspirate
Up/Dispense
Up
Down
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Table 5-4:
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DIAGNOSTICS AND TROUBLESHOOTING
Motor Direction Commands (Continued)
Motor
Designations
Function
C/3
Probe Rotation
D/4
Saline Syringe
E/5
Directional Valve
F/6
G/7
Spare
Spare
H/8
Lyse Syringe
I/9
Spare
J/10
Needle
K/11
Sample Pump
L/12
Diluent Pump
CELL-DYN® 1700 Service Manual
Command
Direction
0
1
0
1
CCW/To RBC cup
CW/To Pre-Mixing
Cup
Down/Aspirate
Up/Dispense
CCW/Dispense
CW/Aspirate
0
1
Down/Aspirate
Up/Dispense
1
0
1
0
1
0
Up/Pierce
Down/Withdraw
CW/Backwash
CCW/Aspirate
CW/Draw Air Gap
CCW/Aspirate
0
1
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Table 5-5:
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DIAGNOSTICS AND TROUBLESHOOTING
Motor Speed Commands
Motor Speed Commands
Command
Speed in Steps
per Second
1
50
2
75
3
283
4
300
5
166
6
200
7
250
8
10
9
151
10
222
11
25
12
182
13
100
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Table 5-5:
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DIAGNOSTICS AND TROUBLESHOOTING
Motor Speed Commands (Continued)
Motor Speed Commands
14
125
15
91
16
67
17
111
Sample Probe Normal Operation
Figure 5-5 illustrates the Sample Probe’s up/down sequence during the INITIALIZE and RUN cycles.
Figure 5-6 shows the probe’s rotation movement during the INITIALIZE cycle.
Initialization Mode
The Initialization cycle places mechanical and electrical components in the “home” position, drains
any liquid in the tubing, Pre-Mixing Cup, and the Mixing Chamber of the von Behrens RBC Transducer to the waste system, then places the instrument in the INITIALIZED state.
Stepper Motor Homing
Homing a stepper motor is the process of setting up the initial position from which all future movement
will be referenced. In the CELL-DYN 1700, this is accomplished by forcing the motor to move against
a physical stop (Hard Stop). When the mechanical assembly, driven by the motor, reaches the Hard
Stop, the stepper motor electrically slips until it is forced to stop.
This mechanical position then becomes the zero reference position for the motor.
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Operation:
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DIAGNOSTICS AND TROUBLESHOOTING
1. The Sample Probe moves up at a fast speed until the Upper Switch (#2) is activated. It is
then changed to a slow speed, and "homed" against the Upper Hard Stop, which is the
metal plate at the top of the Sample Probe Assembly.
2. The probe moves down six steps and the Upper Switch (#2) is checked.
3. The probe moves CCW at a fast speed until the Right Switch (#4) is activated. It is then
changed to a slow speed, and "homed" against the Right Hard Stop, which is the
mounting bracket for Right Switch (#4).
4. The probe moves CW to the Pre-Mixing Cup and Left Switch (#3) is checked. The probe
then moves into the Pre-Mixing Cup.
5. The probe moves up and Upper Switch (#2) is checked.
6. The probe moves CCW to center and down positions; and the Lower Switch (#1) is
checked.
7. This completes the Initialization cycle.
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Run Mode
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DIAGNOSTICS AND TROUBLESHOOTING
Figure 5-7 illustrates the probe’s movements during the RUN cycle.
Operation:
1. When the Start Switch is pressed, 30 µL of sample is aspirated and Lower Switch (#1) is
checked.
2. The Sample Probe then moves up to a position six steps from Upper Hard Stop, and
Upper Switch (#2) is checked.
3. The probe moves CW to Pre-Mixing Cup and Left Switch (#3) is checked.
4. The probe moves CCW eight steps and into the Pre-Mixing Cup, where dispense, probe
shake, and aspiration of RBC sample takes place.
5. The probe then moves up to a position six steps from Upper Hard Stop, and Upper Switch
(#2) is checked.
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DIAGNOSTICS AND TROUBLESHOOTING
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Section 5
DIAGNOSTICS AND TROUBLESHOOTING
ASPIR ATIO N
H AR D STO P
PR E -M IXIN G C U P
8 STE PS
R BC
M IXIN G
C H AM B ER
8 STEPS
ELEC TR O N IC H O M E
D ISPEN SE PO SITIO N
117 STE PS
112 STE PS
C EN TER PO S IT IO N
Figure 5-6 Probe Rotate "INITIALIZE" Mode
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Section 5
DIAGNOSTICS AND TROUBLESHOOTING
PR E-M IXIN G CU P
R BC M IXIN G C H AM BER
H ARD STO P
3
3
AD JU ST
SW. #3 H ER E
DISPENSE POSITIO N
8
3
P RO B E SH A K E
AD JU ST SW. #4 H ER E
D IS P E N SE P O S ITIO N
245
119
112
C EN TER PO SITIO N
Figure 5-7 Probe Rotate "RUN" Mode
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DIAGNOSTICS AND TROUBLESHOOTING
Section 5
6. The probe moves CCW to the Mixing Chamber of the von Behrens RBC/PLT Transducer,
stops three steps from Right Hard Stop, and Right Switch (#4) is checked.
7. The probe moves into the RBC/PLT Mixing Chamber and RBC sample is dispensed.
8. The probe moves up to a position six steps from Upper Hard Stop, and Upper Switch (#2)
is checked.
9. After completion of the count cycle, the probe moves CW to center position.
10. The probe moves down and Lower Switch (#1) is checked.
11. This completes the RUN cycle.
Switch Failure Descriptions
Example of fault reports are shown in the following figures:
Figure 5.8
Figure 5.9
Figure 5.10
Figure 5.11
Lower Switch (#1) Fault Report
Upper Switch (#2) Fault Report
Left Switch (#3) Fault Report
Right Switch (#4) Fault Report
When a switch is checked by the computer and found to be deactivated (open) in normal operation,
the following message will be displayed on the RUN Menu: “Not Ready: SEE DIAGNOSTICS”.
From the MAIN MENU, press [DIAGNOSTICS]. The screen will immediately display one of the Fault
Reports shown in Figures 5-8 through 5-11.
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DIAGNOSTICS AND TROUBLESHOOTING
Section 5
The message <SWITCH: 1 CHECK> indicates that Lower Switch (#1) failed when checked. The
message <* NOT ON ANY SWITCH *> indicates that none of the switches were activated when the
failure occurred. Refer to Figure 5-8.
The message <SWITCH: 2 CHECK> indicates that Upper Switch (#2) failed when checked. The
message <* NOT ON ANY SWITCH *> indicates that none of the switches were activated when the
failure occurred. Refer to Figure 5-9.
The message <SWITCH: 3 CHECK> indicates that Left Switch (#3) failed when checked. The message <ON SWITCH(ES): 2 TOP> indicates that Left Switch (#3) was activated when the failure
occurred. Refer to Figure 5-10.
The message <SWITCH: 4 CHECK> indicates that Right Switch (#4) failed when checked. The message <ON SWITCH(ES): 2 TOP> indicates Right Switch (#4) was activated when the failure occurred.
Refer to Figure 5-11.
The above conditions do not necessarily indicate that a switch has actually failed. They only indicate
that the switch was not read as activated when checked by the computer. A failure could also be
caused by improper switch alignment, an electronic hardware failure, or a mechanical hardware failure.
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DIAGNOSTICS AND TROUBLESHOOTING
Figure 5-8: Lower Switch (#1) Fault Report
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DIAGNOSTICS AND TROUBLESHOOTING
Figure 5-9: Upper Switch (#2) Fault Report
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DIAGNOSTICS AND TROUBLESHOOTING
Figure 5-10: Left Switch (#3) Fault Report
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DIAGNOSTICS AND TROUBLESHOOTING
Figure 5-11: Right Switch (#4) Fault Report
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Section 5
5.10 CELL-DYN 1700 ERROR MESSAGES
DIAGNOSTICS AND TROUBLESHOOTING
Table 5-6 below lists the most serious error messages on the CELL-DYN 1700 instrument.
Table 5-6:
Error Messages
Error Message
(Status box)
Time-out at N
seconds
Description
A CCM process initiated by the user took longer to complete than
allowed (usually indicating a failure of the CCM). The process ran
approximately N seconds before the time-out occurred.
A count test was stopped either by the user or because of a fault
detected by the CCM.
Process
Aborted
Fix then press
[CLEAR
A user-correctable fault condition was detected.
ALARM]
Process MoniA process was stopped by the user (using the asterisk (*) key.
toring Aborted
Error Message Description of the error.
(Display Area II)
The Printer Output option was ON and the printer did not print the
Printer Time-out requested report in the expected time.
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Table 5-6:
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DIAGNOSTICS AND TROUBLESHOOTING
Error Messages (Continued)
Error Message
(Status box)
Code N is
Invalid
Incomplete
Aspiration
Error Message
(Status box)
Cannot do this
Function
WBC Meniscus
Detection, RBC
Meniscus
Detection
WBC Count
Time-out (clog),
RBC Count
Time-out (clog)
Description
The user has entered a command for the CCM whose numeric value
exceeds 127. The value entered was N.
On a CELL-DYN 1700CS, not enough blood was detected for the
last processed sample.
Description
The user has attempted to issue a command to the CCM that cannot
be executed because of a pending fault condition.
During the most recent count, a meniscus was not detected or was
detected at an unexpected time.
During the most recent count, a Clog occurred.
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Table 5-6:
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DIAGNOSTICS AND TROUBLESHOOTING
Error Messages (Continued)
Error Message
(Status box)
CCM Pulse
Height Memory
Saturation
Warning
External Waste
Full
Lyse Empty,
Detergent
Empty, Diluent
Empty
Invalid Alarm
Set
*NOT ON ANY
SWITCH*
Description
During the most recent count, there was an overflow in one of the
pulse-height arrays (histograms).
The external waste bottle has been filled.
The indicated reagent has run out.
A bit was set in the fault message from the CCM that has no valid
interpretation.
After some mechanical motion, a reading of all the position sensors
indicates that none are activated. (This message does not necessarily mean that a mechanical fault has occurred.)
Waste Overflow
A reading of the sensor in the Waste Accumulator suggests that
Into Accumulathere is liquid in the accumulator.
tors
Error Message
Description
(Status box)
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Table 5-6:
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DIAGNOSTICS AND TROUBLESHOOTING
Error Messages (Continued)
Error Message
(Status box)
Vacuum
Pressure
Position Fault
Sensor Fault Internal Waste
Empty
Canceling AutoCycling
Invalid UIC
Command Sent
to CCM
Description
There was a vacuum failure during power-up or the instrument is
unable to maintain vacuum level while in the READY state.
There was a pressure failure during power-up.
A mechanical assembly is not in the correct position for the most
recent function to be performed, as indicated by position sensors.
A Time-Out fault occurred in draining one of the waste bottles. This
error is also associated with positive pressure.
This message appears when the user cancels Auto-Count Testing.
The UIC sent a command to the CCM that it cannot interpret.
Error in Flow
System Timing
An error in the timing of a flow script has occurred. This occurs during instrument initialization when the flow script takes more than
three minutes to complete.
Histogram
Memory Clear
The CCM was unable to clear the pulse-height memory.
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Table 5-6:
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DIAGNOSTICS AND TROUBLESHOOTING
Error Messages (Continued)
Error Message
(Status box)
CCM Program,
RAM Memory
Error Message
(Status box)
CCM/MPM
Message Fault
Description
The CCM detected a failure in its RAM.
Description
Other errorrelated messages:
An error in CCM/MPM interprocessor communications occurred. A
fault was generated in an attempt to send or receive motor or other
MPM to CCM,
Message Trans- command to or from MPM, or the MPM was unable to perform the
function.
mit Error
Command to
be Sent to
MPM is Incorrect
Error Message
(Status box)
Description
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Table 5-6:
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DIAGNOSTICS AND TROUBLESHOOTING
Error Messages (Continued)
Error Message
(Status box)
Description
No Such Script
Error in loading a flow script.
in ROM or RAM
CCM/UIC MesAn error in UIC/CCM interprocessor communications occurred.
sage Fault
DCM Fault
A fault was detected during power-up check of the DCM board.
No Response
from CCM.
The CCM is not functioning or the signal cable connecting the CCM
and UIC is faulty or disconnected. Turn the instrument OFF, check
the CCM/UIC cable, then turn the instrument ON.
CCM is InitializThe CCM is in the middle of its Initialization process.
ing
Undefined
An undefined event or process occurred.
Event
Count Test
The [COUNT TEST] key in the DIAGNOSTICS Menu is used to run specimens and display Count
Check data without returning to the RUN Menu. Coded data relating to specific cycle functions, raw
measurement, and flow count time are displayed for use in troubleshooting or service.
Table 5-7 lists the event messages that are displayed during the Diagnostic Menu Count Test.
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DIAGNOSTICS AND TROUBLESHOOTING
Event Messages During Diagnostic Menu Count Test
Event Messages
Description
SampSw. pressed Touch Plate was pressed.
Remove speciSpecimen should be removed.
men
Count valve open
RBC histogram
avail
WBC upper det
WBC lower det
RBC upper det
RBC lower det
Plt recount strt
CCM initing
Data invalid
WBC histo avail
Proc complete
Data avail
CCM init done
Canceled
The counting valve is open.
RBC histogram is available.
WBC upper meniscus detection.
WBC lower meniscus detection.
RBC upper meniscus detection.
RBC lower meniscus detection.
Platelet recount starts.
CCM initializing.
Data entered is invalid.
WBC histogram is available.
Process is completed.
Data is available.
CCM initialization is completed.
Canceled operation.
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Section 5
Operator-Correctable Alarm or Fault Messages
DIAGNOSTICS AND TROUBLESHOOTING
Table 5-8 lists operator-correctable alarm or fault messages.
Table 5-8:
Operator-Correctable Alarm or Fault Messages
Operator-Correctable
Alarm or Fault Messages
External Waste Full
Detergent Low
Diluent Low
Lyse Empty
Invalid alarm set
Detergent Empty
Diluent Empty
CELL-DYN® 1700 Service Manual
Description
Waste full sensor is activated.
Detergent is low as detected by
reagent sensor in reagent inlet
tube.
Diluent is low as detected by
reagent sensor in reagent inlet
tube.
No lyse is detected by reagent
sensor in reagent inlet tube.
Incorrect error or message was
set.
No detergent is detected by
reagent sensor in reagent inlet
tube.
No diluent is detected by
reagent sensor in reagent inlet
tube.
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Table 5-8:
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Operator-Correctable Alarm or Fault Messages (Continued)
Operator-Correctable
Alarm or Fault Messages
Description
CCM module is currently in the
INITIALIZED state.
CCM is initializing
Summary of Error Messages
Table 5-9 gives a complete listing of error messages available on the CELL-DYN 1700.
Table 5-9:
Summary of Error Messages
Error Message (Status box)
DOS Errors
Arithmetic overflow
Bad drive request structure length
Cannot remove current directory
Cannot rename across drives
Collection index out of range
Collection overflow
CRC error in data
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
Device read fault
Device write fault
Disk full
Disk is write-protected
Disk read
Disk seek
Division by zero
Drive not ready
File access denied
File not assigned
File not found
File not open
File not open for input
File not open for output
Floating point overflow
Floating point underflow
Hardware failure
Heap overflow
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
Invalid drive number
Invalid file access code
Invalid file handle
Invalid floating point operation
Invalid function number
Invalid numeric format
Invalid pointer operation
Path not found
Printer out of paper
Range check
Sector not found
Stack overflow
Too many open files
Unknown command
Unknown media type
Unknown unit
General
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
Demographic entry disabled
No data to print
Stopping
Unable to Load Flowscript
Unable to Save Cal Factors
Unable to Set Volume
System Fault
Not Ready: See DIAGNOSTICS
Uninitialized
Normal Operator-Correctable
Detergent empty
Diluent empty
Lyse empty
Waste full
DLA Faults
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
<- ? Invalid error code
CCM command echoed does not match
CCM REQ1 high during data send at char
CCM REQ1 is stuck LOW
DLA buffer overflow on receiving char
DLA/CCM error on command
Incorrect checksum on CCM data receive
NAK received from CCM
No response from CCM
Time-out 1 on wait for CCM REQ1
Time-out 2 on message send to CCM
Time-out 3 on message receive from CCM
Date
Battery failure or system date/time not set
Disk I/O
Cannot open CD1700. ini configuration file
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
Cannot read CD1700. ini configuration file
Cannot write to CD1700. ini configuration file
CD1700. ini configuration file size error
CD1700. ini configuration file version error
Configuration file error
Error when reading CRC values from disk
Memory error when creating CRC tables
Ticket Printer
Data err
Inc. asp.
No Ticket Detected
QC Log
Can not accept specimen
Can not reject specimen
No loading, QC file has to be empty
Purge log failed
Read QC file failed
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
Data Log
Cannot do if uninitialized
Cannot write Data Log header to disk
Count overrange
Data Log write error
Failed to read from Data Log
Flow err
Initialize
Data Log initialization failed
QC Log initialization failed
Communications
Break interrupt
Comm open error
Comm error
Framing error
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
Parity error
Re-transmit started
Re-transmit time-out
Receiver overrun
Transmit started
Unable to re-transmit
Printer Driver
Printer Fault
Printer Not Ready
Printer Off-line
Printer Out Of Paper
Printer Time-out
Ticket Printer Not Ready
QC Log I/O
Failure to read from QC Log
Failure to write to QC Log
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
QC Log size error
QC Log version error, bytes short for creation of QC
Log
Help
Unable to open help file
Reagent Log
Unable to create file
Unable to open file
Data Log Errors
Cannot Access Data Log
CRC Reading Failed
Data Log initialize failed, bytes short for creation of
Data Log
Data Log Print Error
Data Log size error, Call Technical Support
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
Data Log version error
Data Transmission Error
Not enough memory error
X-B File
Failed to write to Data Log Header
Failed to write to Data Log
No response from CCM
Data Log I/O
Data Log write failure
Write Header failure
Write version error
General Faults
Abnormal time-out / no MPM response
Attempt to send MPM a new command while busy
CCM pulse height memory saturation warning
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
CCM is in Fault State
CCM is in Unknown State
CCM Program, RAM Memory Fault
CCM real time clock has failed
CCM/DLA Message Communication Fault
Command to be sent to MPM is incorrect
Count Overrange
Count time-out (clog)
Data Capture Fault
DCM Fault
Detected Probe Assembly Switch : # in Incorrect
State
Detergent sensor
Diluent sensor
Error in Flow System Timing
Fault response from MPM
Guard electrode voltage warning
Histogram Memory Clear Failure
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
Incomplete Aspiration
Incorrect command to be sent to MPM
Initial Communication with DLA Failed
Invalid Command Sent to CCM
Invalid Data
Mechanical Position Fault
MPM to CCM, message transmit error
No response from MPM
Press [INITIALIZATION] key to clear fault and re-initialize meniscus detector: “True” sensed at metering
start meniscus: not detected during valid time interval
Pressure Level Time-out
Pressure Over-Limit Detected
Printer is not Ready: Cannot RUN uninitialized
RBC Clog
RBC Flow Error
Run time error: # at :
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
Time out after approximately __ minutes
Turn off instrument and drain accumulators manually
Unexpected response from MPM
Unknown MPM/CCM fault
Vacuum Level Time-out
Waste Drain to Empty Time-out
Waste Overflow into accumulators
WBC Clog
WBC Flow Error
Wrong software for this instrument
Fault Log
Error in writing to Fault Log file
Error in writing header to Fault Log file
Fault Log I/O
Allocating heap memory error
Bytes short to create the Fault Log
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Table 5-9:
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DIAGNOSTICS AND TROUBLESHOOTING
Summary of Error Messages (Continued)
Error Message (Status box)
Fault Log file size error
Fault Log Header IO Error
Incorrect version of Fault Log
Lab ID Setup
Drive A is not ready
Fail to read assay file
Fail to write LAB ID file
Incorrect assay format
Incorrect disk. QC values do not apply to this instrument
Incorrect Exp. Date
Incorrect Lot Number
Incorrect parameter in assay file
5.11 SOFTWARE COMMANDS AND SEQUENCE
CD1700 File and Directory Structure
The current CELL-DYN 1700 directory structure is outlined below:
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C:\
COMMAND.COM
AUTOEXEC.BAT
CONFIG.SYS
AUTOEXEC.BAK
CONFIG.BAK
‘
DOS
CD1700
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DIAGNOSTICS AND TROUBLESHOOTING
(root directory)
(currently DOS 5.0)
(backup of original file if created by
previous installation)
(backup of original file if created by
previous installation)
(subdirectory)
(subdirectory)
CD1700.EXE
CD1700.INI
DATALOG (20.5 MB)
QCLOG
(5.5 MB)
FAULTLOG
REAGLOG
ID.DAT
HELP
(subdirectory)
HELPxx (xx is numeric)
Accessing DOS
There are two ways to access the DOS program from the CD1700 program: with the CD1700 program still loaded or by exiting the CD1700 to DOS. The Exit CD1700 to DOS method is the preferred
method.
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Section 5
CD1700 Program Loaded
DIAGNOSTICS AND TROUBLESHOOTING
1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.
2. 2. Press ESC on the PC keyboard. A warning message appears on the screen. Heed
the message.
3. Press the Alt and d keys simultaneously. The following DOS prompt appears:
C:\CD1700>.
4. Execute the desired DOS commands. Most commands can be executed. However,
computer memory is limited since the CD1700 program is still loaded.
CD1700 Program Not Loaded
1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.
2. Press ESC on the PC keyboard. A warning message appears on the screen. Heed the
message.
3. Press the Alt and x keys simultaneously. The CD1700 program is removed from computer
memory. The following DOS prompt appears: C:\>.
4. Execute the desired DOS commands.
Exiting DOS
To exit the DOS Program and return to the CD1700 Program in the case where the CD1700 Program
remains loaded (Alt d was used to access DOS), type exit at the C prompt and press the Enter key.
The DIAGNOSTICS Menu will again be displayed.
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Section 5
To exit the DOS Program in the case where the CD1700 Program is not loaded (Alt x was used to exit
the CD1700 Program), turn the instrument OFF then ON again to reinitialize the system.
NOTE: Turning the instrument OFF then ON again prepares the system for normal operation.
Common DOS Commands
Function
Command
Description
Change drives
Change directory
New directory
c: or a:
cd
md
switch C and A drives
filename
directory name
Remove directory
Compare
Copy
Delete
Directory
Exit
Rename
Undelete
rd
comp
copy
del
dir
exit
ren
undelete
directory name
file1 with file2
source to destination
file name(s)
current or specified drive
quits the current program
file1 to file2
file name
DOS Command Usage
Change Drives
When accessing DOS from the CD 1700 program, the C:\> prompt is displayed, indicating the C drive
is selected. To change to the A drive (floppy disk), type A: and press Enter. The prompt changes to
A:\> indicating the A drive is selected.
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Section 5
Change Directories/Files Within a Drive
DIAGNOSTICS AND TROUBLESHOOTING
To move from a directory to the root directory (C:\) type cd\ and press the Enter key.
To move one level closer to the root directory, type cd.. (where .. are two periods) and press the Enter
key. For example, to move from CD1700\HELP to CD1700 subdirectory, type cd.. and press the
Enter key.
To move one level away from the root directory, type cd_[directory name] (where _ indicates a
space) and press the Enter key. For example, to move from the root directory (c:\) to the CD1700
directory, type cd cd1700 and press the Enter key.
Make a New Directory
To create a new directory or subdirectory, type md (for make directory) followed by a space and the
name of the new directory and press Enter. For example, to create a new directory called Sample,
type md sample and press the Enter key.
NOTE: When creating a subdirectory, make sure you are in the proper directory.
Remove a Directory
To remove a directory, first ensure that there are no files in that directory. All files must be removed or
erased from a directory before that directory can be removed. Type rd_[directory name] (where _
indicates a space) and press the Enter key. For example, to remove an empty directory called
Patient, type rd patient and press the Enter key.
Compare
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Section 5
Use the Compare command to compare the contents of two files. The files can have the same name,
provided they are in different directories or on different drives. To compare two files, type
comp_file1_file2 (where _ indicates a space). For example, to compare the file Sample1 with
Sample2, type
comp sample1 sample2 and press the Enter key.
Copy
To copy a file on the CELL-DYN 1700 hard drive (C drive) to a floppy disk (on the A drive):
1. Access DOS by following the Exit CD1700 Program procedure above. Change to A drive
if the file to be copied is on A drive.
2. If necessary, use the cd command to access the directory containing the file(s) to be
copied.
3. At the C prompt, type Copy_C:\[filename]_A:\ (where [filename] is the complete file
name and _ indicates a space between characters). Example: to copy file CONFIG.SYS
from the C drive to the A drive, type
copy c:\config.sys a:\ and press the Enter key.
To copy this file from the A drive to the C drive, type
copy a:\config.sys c:\ and press the Enter key.
Delete a File
To delete a file:
1. 1. Access DOS by following the Exit CD1700 Program procedure above. Change to A
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drive if the file to be deleted is on A drive.
2. 2. If necessary, use the cd command to access the directory containing the file(s) to be
deleted.
3. 3. At the C prompt, type delete_ [filename] (where [filename] is the complete file name
and _ indicates a space between characters) and press the Enter key. For example, to
delete a file called Sample from the C drive, type delete sample and press the Enter key.
Undelete a File
To undelete a file which had previously been deleted:
1. At the C prompt, type undelete_ [filename] (where [filename] is the complete file name
and _ indicates a space between characters) and press the Enter key. For example, to
undelete a file called Sample from the C drive, type undelete sample and press the Enter
key.
Rename a File
Use the Rename command to change the name of an existing file. To change the name of file, use
the format ren_[filename1]_[filename2] (where _ indicates a space).
For example, to rename the file PATIENT1 to the new name SAMPLE2, type ren patient1 sample2
and press the Enter key.
View Files in a Directory
To view all the files in a particular directory:
1. 1. Access DOS by following the Exit CD1700 Program procedure above. Change to A
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drive if the files to be viewed are on A drive.
2. 2. If necessary, use the cd command to access the directory containing the file(s) to be
viewed.
3. 3. At the C prompt, type dir and press the Enter key.
NOTE: If there are many files, the file names will scroll down the screen too rapidly for the user to see.
The user can prevent this by adding a specified “parameter” to the DIR command using the
following format: DIR_[parameter] (where _ indicates a space).
For example, to stop the file names at the end of each page: type dir /p and press the Enter
key.
To display the file names in columns across the width of the page: type dir /w and press the
Enter key.
Software Installation/Upgrades
1. 1. With the CELL-DYN instrument ON, go to the DIAGNOSTICS Menu.
2. 2. Press ESC on the PC keyboard. A warning message appears on the screen. Heed
the message.
3. 3. Press the Alt and x keys simultaneously. The following DOS prompt appears:
C:\CD1700>.
4. 4. Install the software disk in Drive A.
5. 5. For initial software installation only, type A:\ncs for the 1700 model or A:\cs for the
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1700CS model, and press the Enter key.
NOTE: The ncs and cs commands will create new files, writing over existing files such as Data Log.
For this reason these commands should not be used to install software upgrades. As of the
publication date of this manual, no separate command existed for installing software upgrades.
Check Technical Service Bulletins for any additional information concerning this procedure.
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Section 6
Section 6.
DIAGRAMS AND SCHEMATICS
Table of Contents
6.1 SECTION OVERVIEW
List of Diagrams and Schematics
Title
Metering Tube Printed Circuit
Board (200mL)
Metering Tube Printed Circuit
Board (100mL)
Chopper Driver Printed Circuit
Board
Pre-Amplifier Module (PAM)
Signal Processor Module (SPM)
Main Amplifier Module (MAM)
Pressure Regulator Module (PRM)
Cell Count Module (CCM)
Motor Processor Module (MPM)
Solenoid Driver Module (SDM)
Device Control Module (DCM)
Cable Distribution Module (CDM)
CELL-DYN® 1700 Service Manual
Part Number
9630102
9630105
963042X
963045X
9630522
9630531
9630801
9630810
9630920
9630930
9630940
9630950
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Section 6
Title
Mother Board Module (MBM)
Data Link Adapter (DLA)
Power Distribution Module (PDM)
Keyboard Interconnect
CELL-DYN 1700CS Flow Diagram
Wiring Diagram, Cable Connection for
CELL-DYN 1700/1700CS
CELL-DYN® 1700 Service Manual
DIAGRAMS AND SCHEMATICS
Part Number
9631291
9631540
9631560
9631580
9480081
9510056
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6.1 SECTION OVERVIEW
DIAGRAMS AND SCHEMATICS
This section contains diagrams and schematics for the CELL-DYN 1700 and 1700CS.
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Section 7
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Section 7.
Removal and Replacement
Table of Contents
7.1 INTRODUCTION
7.2 SAFETY PRECAUTIONS
Decontamination
Decontamination Procedures
Printed Circuit Board Handling
7.3 SERVICE EQUIPMENT REQUIRED
7.4 DISASSEMBLY/REPLACEMENT PROCEDURES
Model 1700CS Upper Front Cover Removal
Model 1700 Upper Front Cover Removal
Model 1700CS Lower Front Cover Removal
Model 1700 Lower Front Cover Removal
Top Cover Removal
Bezel Removal
Right Cover Removal
Left Cover Removal
RBC and WBC Aperture Plate Removal
von Behrens WBC and RBC/PLT Transducer Removal
WBC and RBC Metering Board Removal
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REMOVAL AND REPLACEMENT
Hemoglobin Flow Cell Removal
Sample Probe Driver Assembly Removal
PAM (Pre-Amplifier Module) Removal
Diluent Syringe Driver Assembly Removal
Diluent Syringe Removal
Sample Syringe Driver Assembly Removal
Sample Syringe Removal
Lyse Syringe Driver Assembly Removal
Lyse Syringe Removal
Fluid Power Supply Removal
MPM (Motor Processor Module) Board Removal
CDM (Cable Distribution Module) Board Removal
CRT Assembly Removal
CPU Assembly Removal
PC Power Supply Removal
I/O Boards Removal -- DLA; SVGA, Ticket Printer, and Graphics
Printer/Disk Controller
CPU Motherboard Removal
Disk Drive Assembly Removal
Main (Linear) Power Supply Removal
Cage Mounted Plinted Circuit Boards Removal
Switching Power Supply Removal
Needle Drive Assembly Removal -- Closed Sample Module
Chopper Driver Board Removal -- Closed Sample Module
Sample and Diluent Pumps Removal -- Closed Sample Module
Fan Air Filter Removal
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Section 8
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Section 8.
ALIGNMENT AND VERIFICATION PROCEDURES
Table of Contents
8.1 SECTION OVERVIEW
8.2 TEST EQUIPMENT AND SUPPLIES REQUIRED
8.3 PREPARATION FOR ALIGNMENT/VERIFICATION
8.4 ORDER OF ALIGNMENT/VERIFICATION
8.5 VACUUM AND PRESSURE ADJUSTMENTS
Regulator Alignment
Pressure Adjustment (0.5 psi)
Pressure Verification (High)
Vacuum Adjustment (8 inch)
8.6 METERING SYSTEM TIMING ADJUSTMENTS — RBC AND WBC
RBC Metering System Timing Adjustment
WBC Metering System Timing Adjustment
8.7 24V SWITCHING MODULE ADJUSTMENT8.8 POWER DISTRIBUTION MODULE TEST POINTS
8.9 CABLE DISTRIBUTION MODULE TEST POINTS
8.10 SIGNAL PROCESSOR MODULE ADJUSTMENT
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Section 8
ALIGNMENT AND VERIFICATION
8.11 DEVICE CONTROL MODULE ADJUSTMENT
8.12 PRE-AMPLIFIER MODULE ADJUSTMENT
Pre-Amplifier Module (PAM) Test Points
Pre-Amplifier Module Adjustment
8.13 MAIN AMPLIFIER OFFSET and GAIN ADJUSTMENT ALIGNMENT
WBC Offset
WBC Gain
RBC Offset
RBC Gain
RER Adjustment
PLT Offset
PLT Gain
8.14 DILUENT AND SAMPLE VERIFICATION/ADJUSTMENT
Diluent Volume Verification
Sample Volume Verification
8.15 SAMPLE PROBE ALIGNMENT PROCEDURES
Stepper Motor Power Test and Verification
Lower Microswitch #1 Adjustment
Upper Microswitch #2 Adjustment
Left Microswitch #3 Adjustment
Right Microswitch #4 Adjustment
8.16 DILUENT SYRINGE CALIBRATION BLOCK ADJUSTMENT
8.17 SHORT SAMPLE SENSOR (1700CS) ADJUSTMENT
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ALIGNMENT AND VERIFICATION
List of Tables
Table 8-1 Metering Timing Fault Report
Table 8-2 PDM Test Points
Table 8-3 CDM Test Points
Table 8-4 SPM Alignments
Table 8-5 DCM Alignment
Table 8-6 PAM Test Points
Table 8-7 Pre-Amp Alignments
List of Figures
Figure 8-1 CELL-DYN 1700 Metering Timing Chart
Figure 8-2 RBC Metering Tube
Figure 8-3 WBC Metering Tube
Figure 8-4 PDM Test Points
Figure 8-5 SPM Test Points
Figure 8-6 DCM Test Points
Figure 8-7 PAM Test Points
Figure 8-8 MAM Test Points
Figure 8-9 WBC Gain Adjustments
Figure 8-10 RBC Gain Adjustments
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Section 8
Figure 8-11 Cell Edit Chart
Figure 8-12 Editing Ratio Displays
Figure 8-13 PLT Gain Adjustments
Figure 8-14 Diluent Syringe
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ALIGNMENT AND VERIFICATION
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Section 8
8.1 SECTION OVERVIEW
ALIGNMENT AND VERIFICATION
This chapter discusses the alignment and verification procedures for the CELL-DYN 1700. These
procedures are used to ensure the proper electronic alignment of the circuitry.
These procedures also serve as a method of isolating a defective assembly, module, or printed circuit
board.
Service representatives must ensure that all external components of the system, such as reagents,
blood samples used, controls and calibrators, environment, and AC power, are acceptable and correct before proceeding with the alignment and verification procedures.
WARNING: Potential Biohazard. Consider all specimens and reagents,
controls, calibrators, etc. that contain human blood or serum as potentially
infectious. Use established, safe laboratory working procedures when handling these samples. Wear gloves, lab coats, and safety glasses, and follow
other biosafety practices as specified in the OSHA Bloodborne Pathogen
Rule (29 CFR Part 1910, 1030) or other equivalent biosafety procedures.
8.2 TEST EQUIPMENT AND SUPPLIES REQUIRED
ITEM
1
3
4
QTY
1
3
1
DESCRIPTION
DIGITAL VOLTMETER
5-inch JUMPER LEADS
STOPWATCH
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ITEM
QTY
DESCRIPTION
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
1
1
1
1
1
3
1
AR
1
AR
AR
1
1
1
AR
AR
AR
1
1
1
VACUUM GAUGE 0-30 INCHES
PRESSURE GAUGE 0-5 lb.
PRESSURE GAUGE 0-10 lbs.
LATEX SPHERES 5.0 or 5.01 DIA.
LATEX SPHERES 3.31 DIA.
HEMOSTATS
500 mL FLASK OR BEAKER
SILICONE TUBING
STANDARD TOOL KIT
FRESH BLOOD SAMPLES WITH REFERENCE VALUES
ASSAYED CONTROLS FOR CELL-DYN 1700
20K OHM 1% RESISTOR
15K OHM 1% RESISTOR
10 mL GRADUATED CYLINDER
40
100
50 mL COUNTING CUPS
25 mL GRADUATED CYLINDER
12-INCH RULER WITH 1/16 INCREMENTS
OSCILLOSCOPE (OPTIONAL)
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Section 8
8.3 PREPARATION FOR ALIGNMENT/VERIFICATION
ALIGNMENT AND VERIFICATION
Perform the following procedure to prepare the CELL-DYN 1700 for alignment/verification:
1.
Verify all reagents are correct and available in sufficient quantities to perform 100-150
cycles on the instrument.
2.
Remove the upper and lower front covers, left and right side covers, and top cover.
3.
Remove and clean both RBC/PLT and WBC aperture plates following the procedure in
Section 9: Service and Maintenance, Subsection: Aperture Plates Cleaning of the
CELL-DYN 1700 Operations Manual.
4.
Clean the HGB Flow Cell following the procedure in Section 9, Subsection: HGB Flow
Cell Manual Cleaning of the CELL-DYN 1700 Operations Manual.
5.
Re-initialize the instrument by turning the system OFF then ON again. When
Initialization is complete, press [PRIME/RUN] to prime the instrument. Observe the flow
system for leaks, tubing placement, pinched tubing, etc.
6.
Reinstall the covers on the instrument.
7.
Run a Background count. Verify all background values are within the following
specifications:
8.
WBC < 0.5 K/µL
RBC
< 0.05 M/µL
HGB
< 0.1 g/dL
PLT
< 10.0 K/µL
Enter the date and time according to directions in Section 2: Installation Procedures
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ALIGNMENT AND VERIFICATION
and Special Requirements, Subsection: Date/Time Key of the CELL-DYN 1700
Operations Manual.
In the MAIN MENU, press [CALIBRATION]. Record all Calibration Factors for Open,
Closed, and Pre-Dilute modes.
10. Type “94043” to display the DILUTION FACTORS screen. Record all Dilution Factors.
11. In the MAIN MENU, type “999” for Operator ID and press Enter on the PC keyboard or
membrane keypad. This is to identify all runs performed by service personnel.
8.4 ORDER OF ALIGNMENT/VERIFICATION
The following procedures are presented to ensure proper alignment of the CELL-DYN 1700.
1.
Vacuum and Pressure Adjustment (Section 8.5)
2.
RBC Count Time Adjustment (Section 8.6)
3.
WBC Count Time Adjustment (Section 8.6)
4.
24V Switching Module Adjustment (Section 8.7)
5.
Power Supply Voltage Checks and Adjustment (Section 8.8)
6.
Signal Processor Module Alignment (Section 8.10)
7.
Device Control Module Alignment (Section 8.11)
8.
Pre-Amplifier Module Alignment (Section 8.12)
9.
Main Amplifier Module Alignment (Section 8.13)
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10. Diluent/Sample Verification/Alignment (Section 8.14)
11. Sample Probe Microswitch Alignment (Section 8.15)
12. Diluent Syringe Calibration Block Adjustment (Section 8.16)
13. Short Sample (1700CS) Sensor Adjustment (Section 8.17)
When performing adjustments:
At the completion of each Vacuum and Pressure Adjustment procedure, verify the WBC and
RBC count times and normal system operation
Prior to starting any electronic alignment, perform the Power Supply Voltage Check procedure Sections 8.7 and 8.8)
Whenever an alignment adjustment is performed on the Pre-Amplifier Module (PAM), verify
the affected parameters on the Main Amplifier Module (MAM). For example, if PLT is adjusted on the PAM, then PLT should also be checked on the MAM.
8.5 VACUUM AND PRESSURE ADJUSTMENTS
The CELL-DYN 1700 utilizes one vacuum and two pressure levels to accomplish the following tasks:
moving sample, reagents, and waste, bubble mixing of sample, and backflushing RBC and WBC
apertures. The vacuum and bubble mix pressures are adjustable by a solid-state regulator. The
backflush pressure is not critical and the pump is under direct computer control.
The solid state regulator has two input ports: P1 for pressure and P2 for vacuum. It also has jumper
terminals which accommodate all desired vacuum and pressure ranges.
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Section 8
The jumper positions are:
1.
2.
3.
4.
Jumpers
Pressure (psi)
Vacuum (inches Hg)
A-B
C-D
E-F
G-H
0.0 - 3.0
2.5 - 5.1
4.3 - 6.6
6.0 - 8.0
0.0 - 6.0
5.0 - 10.2
8.6 - 13.2
12.0 - 16.0
ALIGNMENT AND VERIFICATION
Regulator Alignment
This offset adjustment must be performed with no pressure or vacuum applied to the regulator. Follow the steps below:
1.
Disconnect the appropriate pumps on the pump relay module and bleed off pressure
from the accumulators.
•
•
J4 for low pressure
J2 for vacuum on new-style pumps
2.
Remove the pressure or vacuum line from the top of the regulator.
3.
On the Pump Relay Board, the voltage should be 5.0 +/- 0.15 volts at J7 pin 7 for
vacuum, and J8 pin 5 for pressure.
NOTE: You must remove the board mounting screws and free the rest of the board to make the remaining adjustments. Disconnect J6 or J7 while relocating the appropriate printed circuit board
to avoid shorting out components.
4.
Note the current position of jumper E1 and set E1 to the C-D position.
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5. Connect the DVM (Digitial Volt Meter) negative lead to TP3 (GND) on regulator board.
Connect the positive lead to TP2 (REF). The voltage should be 1.00V +/- 0.14 volts.
• If voltage is not correct, re-check step 3.
• If voltage is still not correct, replace the regulator.
6. Connect the DVM negative lead to TP1 and the positive lead to TP2, and read the
voltage. Adjust R18 for a voltage of 0.000 and +/- 0.005.
NOTE: If voltage is negative, turn R18 (offset) clockwise; if the voltage is positive, turn R18 counterclockwise.
7.
Move jumper E1 back to the proper operating position.
8.
Reconnect the pressure or vacuum line to the top of the regulator and reconnect the
cable(s) on the pump relay module.
Pressure Adjustment (0.5 psi)
Follow the steps below for pressure adjustment:
1.
Remove the top cover and raise the top inner panel (refer to Section 7 for removal
instructions).
2.
Locate the small silicone tubing connected to the in-line fitting at top of the 0.5 psi
Pressure Accumulator (located closest to the rear of the instrument).
3.
Connect a 0-5 psi gauge in-line with the silicone tubing and fitting (the Pressure Accumulator is located on the part of the Fluid Power Supply which is closest to the
rear of the instrument).
4.
Adjust R16 on the Regulator board for 0.5 psi + 0.0 and - 0.05.
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5. Remove the gauge and reconnect the silicone tubing to in-line connector.
Pressure Verification (High)
Follow the steps below for pressure verification:
1.
Locate solenoid valve 1-6 and trace the tubing back through the flow panel to the in-line
connector.
2.
Connect a 0-30 psi gauge in-line.
3.
In the RUN screen, press [CLEAR ORIFICE] while observing the gauge.
4.
When the pump activates, verify a pressure of no less than 4 psi. If the pressure is less
than 4 psi, check for leaks. Replace the Pressure Pump if necessary.
Vacuum Adjustment (8 inch)
Follow the steps below for solid state regulator adjustment:
NOTE: This is a coarse adjustment only. The fine adjustment is predicated upon metering system
count times (Section 8.6). Make this coarse adjustment only if there is reason to believe that
the vacuum is grossly misadjusted.
1.
Locate the solid state vacuum regulator on the rear side of the Fluid Power Supply (left
front side closest to the flow panel).
2.
Remove the TYGON® tubing from the top of the Accumulator (located on the back of
the Fluid Power Supply closest to the flow panel) and connect a 0-30" Hg gauge in-line
between the tubing and the Accumulator.
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3. Adjust R16 (accessible only from the front of the Fluid Power Supply) for
8" Hg +/- 0.2" Hg.
NOTE: Clockwise adjustment increases vacuum and counterclockwise adjustment decreases
vacuum.
4.
Once R16 is adjusted, remove the vacuum gauge and reconnect the tubing line to
the regulator.
8.6 METERING SYSTEM TIMING ADJUSTMENTS — RBC AND WBC
The instrument uses the Volumetric Metering process to regulate the count cycle and to ensure that a
precise volume of sample is analyzed for the measurement. Table 8-1 shows the results of a fault
report displayed on the screen if a flow error or clog occurs during a Run cycle. Figure 8-1 illustrates
the timing relationships for WBC and RBC measurements.
For each transducer there are two distinct counting periods, T1 and T2. Figures 8-2 and 8-3 illustrate
the counting periods for the RBC and WBC metering tubes, respectively.
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Clog Time
(Detector
Masked)
(in seconds)
Flow Error Time
(in seconds)
Valid Meniscus
Time
(in seconds)
Clog Time
(in seconds)
0 - 0.5
0.5 - 1.0
1.0 - 3.0
3.0+
0 - 3.5
3.5 - 4.0
4.0 - 6.5
6.5+ or greater
than the moving
average
0 - 3.0
3.0 - 4.0
4.0 - 6.0
6.0+
6.0 - 7.5
7.5+ or greater
than the moving
average
WBC Lower
RBC/PLT Upper
RBC/PLT Lower
0 - 5.5
Table 8-1:
5.5 - 6.0
Metering Timing Fault Report
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All count times are based on the vacuum level of the 8-inch vacuum reservoir and tubing length.
These count times are critical in that all clog and flow system alarms are generated via these count
times. The vacuum adjustment (refer to Section 8.5) sets the coarse vacuum requirement, but further
adjustments will be necessary to ensure proper timing for the flow system alerts.
RBC Metering System Timing Adjustment
Follow the steps below for adjusting the timing of the RBC metering system:
1.
Ensure that the RBC aperture plate has been removed, cleaned, and reinstalled
following the procedure in Section 9: Service and Maintenance, Subsection:
Aperture Plates Cleaning of the CELL-DYN 1700 Operations Manual.
2.
Run a background count and verify that the RBC displayed count time (T2) is 6.7
seconds +/- 0.2 seconds. Readjust the vacuum to correct the count time if the time is out
of specification (refer to step 3 of the Vacuum Adjustment (8-inch) procedure previously
discussed).
3.
From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].
4.
Observe the time displayed for RBC UPTIME. This time represents (T1) which is the
time when the RBC valve 1-2 opens until the meniscus reaches the upper detector.
RBC UPTIME (T1) should be 4.8 to 5.2 seconds. (The time is displayed in milliseconds.)
5.
Raise the metering tube to increase the upper time; lower the metering tube to decrease
the upper time. This is necessary when the RBC UPTIME is outside the acceptable
range.
6.
Repeat steps 2 through 5 until the RBC Up Time/Count Time is within specification.
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WBC Metering System Timing Adjustment
ALIGNMENT AND VERIFICATION
Follow the steps below for adjusting the timing of the WBC metering system:
NOTE: The RBC count times (T1 & T2) must be within specification before performing this procedure.
1.
Remove and clean the WBC aperture plate as described in Section 9: Service and
Maintenance, Subsection: Aperture Plates Cleaning of the CELL-DYN 1700
Operations Manual.
2.
Run a background count and observe the count time displayed to the right of the WBC
histogram. This time should be 5.0 seconds +/- 1.0 second.
NOTE: There is no count time adjustment procedure. If the count time is out of specification, call the
CELL-DYN Technical Support Center for additional information.
3.
From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].
4.
Observe the time displayed for WBC UPTIME. This time represents (T1) which is the
time when the WBC valve 4-3 opens until the meniscus reaches the upper detector.
WBC UPTIME (T1) should be 1.8 to 2.2 seconds. See Figure 8-3.
5.
If WBC UPTIME is outside the acceptable range, an adjustment will be necessary.
Raise the metering tube to increase the upper time; lower the metering tube to decrease
the upper time.
6.
Repeat steps 2 through 5 until WBC UPTIME/COUNT TIME is within specification.
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Section 8
8.7 24V SWITCHING MODULE ADJUSTMENT
ALIGNMENT AND VERIFICATION
The 24V Switching Module is located next to the fan behind the CRT assembly.
Adjust the regulated +24V supply to 24.5 +/- 0.5V.
Location
Function
Voltage
BROWN
+24V
25.0V +/- 1.0
NOTE: The 24V Switching Module has only one potentiometer. Adjust the potentiometer with an insulated screwdriver and record the adjustment.
8.8 POWER DISTRIBUTION MODULE TEST POINTS
Table 8-2 lists the test points located on the Power Distribution Module (PDM) and Figure 8-4 illustrates the test points on the PDM board.
Test
Function
Range
Ripple
N/A
N/A
TP2
TP3
TP4
TP5
Analog
Ground
+12V
-12V
+24V
+5V
Table 8-2:
PDM Test Points
TP1
CELL-DYN® 1700 Service Manual
+12V +/- 0.6V
< 100mV
-12V +/- 0.6V
< 100mV
+25V +/- 1.0V
< 100mV
+5.15V +/- 0.25V < 100mV
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8.9 CABLE DISTRIBUTION MODULE TEST POINTS
ALIGNMENT AND VERIFICATION
Table 8-3 lists the test points located on the Cable Distribution Module (CDM).
Location
(J5 and J8)
Function
Voltage
Ripple
Brown
Blank
Ground
N/A
< 0.05V
N/A
N/A
N/A
Orange
Yellow
Green
Blank
Ground
+12V Unreg.
+24V Unreg.
N/A
< 0.05V
16.5V +/- 1.0V
34.5V +/- 2.0V
N/A
N/A
3.5V P-P
7V P-P
N/A
Table 8-3:
CDM Test Points
8.10 SIGNAL PROCESSOR MODULE ADJUSTMENT
The Signal Processor Module (SPM), located in the card cage on the right side of the instrument, contains the circuitry for the RBC and WBC lower fixed discriminators and the lower and upper platelet
discriminators. A discussion of the functions of the SPM can be found in Section 4 of this manual;
however, the only field adjustments recommended on this board are the discriminator voltages.
Follow the steps below to align the SPM:
1.
Verify that the instrument is in the READY state.
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2. Connect the DVM positive lead to TP10 (WBC Discriminator) on the SPM. Connect the
ground lead to TP15.
3.
Adjust R44 for 0.15 volts +/- 0.01 volts.
4.
Connect positive lead of DVM to TP11 (RBC Discriminator).
5.
Adjust R45 for 0.35 volts +/- 0.01 volts.
6.
Connect positive lead of DVM to TP19 (PLT High Discriminator).
7.
Adjust R48 for 3.50 volts +/- 0.05 volts.
8.
Connect the positive lead of DVM to TP20 (PLT Low Discriminator).
9.
Adjust R49 for 0.20 volts +/- 0.01 volts.
Table 8-4 contains the specifications for the SPM alignment.
Function
WBC
DISC.
RBC DISC.
PLT HIGH
PLT LOW
Table 8-4:
Test
Adjust
Setting/Range
TP10
R44
0.15V +/- 0.01
TP11
TP19
TP20
R45
R48
R49
0.35V +/- 0.01
3.50V +/- 0.05
0.20V +/- 0.01
SPM Alignments
Test points for the SPM are shown in Figure 8-5.
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8.11 DEVICE CONTROL MODULE ADJUSTMENT
ALIGNMENT AND VERIFICATION
The Device Control Module (DCM) is located in the main electronics card cage. The DCM has a single adjustment that can be performed in the field. The adjustment is for the D to A converter output.
No other adjustments are required.
Follow the steps below to align the DCM:
1.
Verify the instrument is in the READY state.
2.
Connect the DVM positive lead to TP3 on the DCM. Connect the ground lead to TP2
(DAC GND) on the DCM board.
3.
From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times. Press
[SERVICE DEC CODE], type ”2” and press Enter.
4.
Adjust R1 for 9.0 volts +/- 0.07 volts.
5.
Press [SERVICE DEC CODE], type “1” and press Enter.
6.
Check TP3 for 4.5 volts +/- 0.07 volt.
NOTE: Counterclockwise rotation increases the voltage.
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Section 8
Table 8-5 contains the specifications for the DCM alignment.
Function
Test
Adjust
Setting/Range
D TO A
OUTPUT
TP3
R1
9.00 V +/- 0.07
Table 8-5:
DCM Alignment
ALIGNMENT AND VERIFICATION
Test points for the DCM are shown in Figure 8-6.
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Section 8
8.12 PRE-AMPLIFIER MODULE ADJUSTMENT
ALIGNMENT AND VERIFICATION
The Pre-Amplifier Module (PAM) is located on the upper right corner of the Flow Panel. Hemoglobin
circuitry, and the PLT and WBC aperture currents require verification and/or adjustment on this module. The HGB Flow Cell should be cleaned before performing hemoglobin alignments (refer to Section 9, Subsection: HGB Flow Cell Manual Cleaning in the CELL-DYN 1700 Operations Manual).
Pre-Amplifier Module (PAM) Test Points
Remove the top cover, upper and lower front covers, bezel, and the Pre-Amp cover plate (refer to the
PAM Removal procedure in Section 7 of this manual).
Table 8-6 lists the test points located on the PAM.
Location
Function
Voltage
TP5
J3 PIN 1
TP6
TP7
Analog Ground
+100V
+15V
-15V
N/A
+100V +/- 7V
+15V +/- 0.6V
-15V +/- 0.6V
Table 8-6:
PAM Test Points
Pre-Amplifier Module Adjustment
Test points for the PAM are shown in Figure 8-7. Follow the steps below to align the PAM:
1.
Remove the top cover, upper and lower front covers, and bezel (refer to Section 7 of this
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manual for removal instructions).
2.
Locate the PAM mounted on the upper right corner of the flow panel.
3.
Remove the PAM cover (refer to Section 7 for removal instructions).
4.
Connect a jumper between pin 5 of J1 and TP5 (Analog Ground). This will extinguish
the HGB LED.
5.
Connect the positive lead of DVM to TP2. Connect the ground of DVM to TP5.
6.
Adjust R5 (HGB Zero Offset) for 0.000 volts +/- 0.001 volts.
7.
Remove the jumper lead and allow a five-minute warm-up period.
8.
Press the Touch Plate to cycle the instrument and fill the HGB Flow Cell with fresh
reagent.
9.
Measure the voltage at TP2.
10. Adjust R12 (HGB Gain Adjust) for 5.0 volts +/- 0.2 volts.
11. Connect the positive lead of DVM to TP1. Run a background count. Verify 100 volts +/2.0 volts.
12. Connect the jumper between TP8 (Aperture Current Jumper) and TP5 (Analog Ground).
13. Disconnect the RBC signal cable from J2.
14. Connect a 20K Ohm 1/4 watt resistor across pins 4 and 5 of J2.
15. Connect the DVM across the resistor.
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16. Connect the jumper between TP8 (Aperture Current Jumper) and TP5 (Analog Ground).
17. Adjust R21 (PLT aperture current adjust) for 11.0 volts +/- 0.01 volts.
18. Remove the jumper between TP8 and TP5.
19. Remove the resistor and reconnect cable to J2.
20. Disconnect the WBC signal cable from J4.
21. Connect a 15K Ohm 1/4 watt resistor across pins 4 and 5 of J4.
22. Connect the DVM across resistor.
23. Connect the jumper between TP8 (Aperture Current Jumper) and TP5 (Analog Ground).
24. Adjust R35 (WBC aperture current adjust) for 12.0 volts +/- 0.01 volts.
25. Remove the jumper between TP8 and TP5.
26. Remove the resistor and reconnect cable to J4.
27. Reinstall the top cover, upper and lower front covers, and bezel.
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Table 8-7 contains the specifications for the Pre-Amp Module alignment.
HGB ZERO
TP2
HGB GAIN
PLT APER.
CURRENT
WBC APER.
CURRENT
TP2
R12
DUMMY
R21
RESISTOR
DUMMY
R35
RESISTOR
Table 8-7:
R5
0.00 V. +/- 0.001
Jumper to Ground
5.0 V. +/- 0.2 V.
11.0 V. +/- 0.01 V.
12.0 V. +/- 0.01 V.
Pre-Amp Alignments
8.13 MAIN AMPLIFIER OFFSET and GAIN ADJUSTMENT ALIGNMENT
The Main Amplifier Module (MAM) is located in the main electronics card cage. Alignment of the
WBC, RBC, and PLT gains are critical adjustments that must be verified and/or adjusted before instrument accuracy can be established.
Uniform Latex particles are used to perform these adjustments. The particles must be mixed vigorously before diluting to obtain accurate results.
The Gain and RBC Cell Editing adjustments are performed in the Gain Adjust Mode, which allows
multiple counts to be run on the same sample. When Gain Adjust Mode is entered, whatever is in the
Pre-Mixing Cup is transferred to the WBC Cup and 5 mL of diluent is left undisturbed in the RBC Cup.
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When performing RBC or PLT adjustments only, 10 mL of diluent must be placed in Pre-Mixing Cup
before entering Gain Adjust Mode to prevent air from being pulled into the von Behrens WBC Transducer during a count cycle.
Test points for the MAM are shown in Figure 8-8.
Follow the steps below to align the main amplifier offset and gain adjustment.
WBC Offset
1.
Ensure that the instrument is in the READY state.
2.
Connect the DVM Pos lead to TP10. Connect the ground to TP9.
3.
Adjust R1 (WBC ZERO ADJUST) so that the baseline of the noise signal is positioned at
0.000 volts +/- 0.002.
WBC Gain
1.
Prepare a WBC latex dilution.
a. Obtain a clean container. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice to display the [10 mL DISPENSE] key. Press [10 mL DISPENSE] four times to dispense 20 mL of diluent into the container (must press twice to
dispense 10 mL).
b. Add 1 drop of well mixed 5.0 latex particle solution into the 20 mL of diluent and mix
well.
c. Before entering the Gain Adjust mode, use the WBC latex dilution prepared in step
(b) above to fill the Pre-Mixing Cup up to the level of the diluent inlet port.
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2. From the main MAIN Menu, press [RUN] followed by [SPECIMEN TYPE] then the # key
to active the Gain Adjust mode. The message <PREPARING FOR GAIN ADJUST.> is
displayed and WBC dilution is transferred to the WBC Mixing Chamber. When the
preparation cycle is complete, the RUN screen is again displayed.
3.
Press the Touch Plate to run a cycle.
NOTE: Always return to the RUN screen to run a cycle.
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NOTE: When the procedure is completed, or if there is a need to exit the Gain Adjust Mode while performing this procedure, such as constant clogs or improper dilution ratio, press [SPECIMEN
TYPE] followed by [PATIENT SPECIMEN].
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NOTE: The WBC count should be between 10.0 and 30.0. If the count is outside this range, modify
the dilution ratio of the latex particles, exit Gain Adjust Mode and try again.
4.
From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].
5.
Press [SMOOTHING OFF]. The key changes to [SMOOTHING ON] and is highlighted,
indicating the smoothing function is ON. Press [WBC HISTOGRAM]. Observe the
lower part of the histogram data to determine the channel number for peak of 100. If the
gain is adjusted properly, the peak count of 100 will be in channel 56 +/- 1 channel.
NOTE: Turning R4 clockwise will increase the channel number; turning R4 counterclockwise will decrease the channel number.
6.
If the peak count of 100 is not within specification, adjust R4. Return to the RUN screen
and run another cycle. Observe the channel number for peak count of 100. If the gain is
still outside of specification, repeat this process until the peak count of 100 is in channel
56 +/- 1 channel. Refer to Figure 8-9.
7.
If only WBC Gain is to be checked, exit the Gain Adjust mode, go to the RUN Menu, and
press [SPECIMEN TYPE] followed by [PATIENT SPECIMEN].
RBC Offset
1.
Connect a DVM to TP6 (RBC OUT) and the ground to TP9.
2.
Adjust R11 (RBC ZERO ADJUST) so that the baseline of the noise signal is positioned
at 0.00 volts +/- 0.002 volts.
3.
Connect a DVM to TP13 (RBC DISCRIMINATOR) and the ground to TP9.
4.
Adjust R71 (RBC DISC. ADJUST) for 0.40 volts +/- 0.01 volts.
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RBC Gain
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1.
If in the Gain Adjust mode, add 1 drop of well-mixed 5.0 latex particle solution to the 5
mL of diluent already in the RBC Mixing Chamber. Go to step 3.
2.
If not in the Gain Adjust mode, do the following:
a. Pour 10 mL of diluent into the Pre-Mixing Cup and add 1 drop of well-mixed 5.0 latex
particle solution to the 5 mL of diluent already in the RBC Mixing Chamber.
b. In the RUN screen, press [SPECIMEN TYPE] then the # key to activate the Gain
Adjust mode. The message <PREPARING FOR GAIN ADJUST.> is displayed and
diluent in the Pre-Mixing Cup is transferred to the WBC Mixing Chamber. When the
preparation cycle is complete, the RUN screen is again displayed.
3.
Press the Touch Plate to run a cycle.
NOTE: Always return to the RUN screen to run a cycle.
NOTE: The RBC count should be between 3.00 and 6.00. If the count is outside this range, adjust the
dilution ratio of the latex particles by adding more diluent or latex to the RBC Mixing Chamber.
4.
From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].
5.
Press [SMOOTHING OFF]. The key changes to [SMOOTHING ON] and is highlighted,
indicating the smoothing function is ON. Press [RBC HISTOGRAM]. Observe the lower
part of the histogram data to determine the channel number for peak of 100. If the gain
is adjusted properly, the peak count of 100 will be in channel 98 +/- 2 channels. Refer to
Figure 8-10.
NOTE: Turning R9 clockwise will increase the channel number; turning R9 counterclockwise will decrease the channel number.
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6. If the peak count of 100 is not within specification, adjust R9. Return to the RUN screen
to run another cycle. Observe the channel number for peak count of 100. If the gain is
still outside of specification, repeat this process until the peak count of 100 is in channel
98 +/- 2 channels.
7.
Leave SMOOTHING ON.
8.
From the RBC HISTOGRAM screen, press [MORE] twice followed by [SERVICE DEC
CODE]. Type in "101" and press Enter to view High Current Histogram. The Peak
(Mode) should be in channel 98 +/- 2 channels.
9.
If the peak count of 100 is within specification, this procedure is completed.
10. If the peak count of 100 is not within specification, adjust R8. Return to the RUN screen
and run another cycle. Repeat steps 4 through 8.
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RER Adjustment
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ALIGNMENT AND VERIFICATION
1.
If in the Gain Adjust mode, add 1 drop of well-mixed 5.0 latex particle solution to the 5
mL of diluent already in the RBC Mixing Chamber. Go to step 3.
2.
If not in the Gain Adjust mode, do the following:
a. Pour 10 mL of diluent into the Pre-Mixing Cup and add 1 drop of well-mixed 5.0 latex
particle solution to the 5 mL of diluent already in the RBC Mixing Chamber.
b. In the RUN screen, press [SPECIMEN TYPE] then the # key to active the Gain
Adjust mode. The message <PREPARING FOR GAIN ADJUST.> is displayed and
diluent in the Pre-Mixing Cup is transferred to the WBC Mixing Chamber. When the
preparation cycle is complete, the RUN screen is again displayed.
3.
Press the Touch Plate to run a cycle. Make a note of the RBC results.
NOTE: The RBC count should be between 3.00 and 6.00. If the count is outside this range, adjust the
dilution ratio of the latex particles by adding more diluent or latex to the RBC Mixing Chamber.
4.
Repeat steps 1 and 2 above two more times for a total of 3 runs. Calculate the average
of the three RBC counts.
5.
Refer to Figure 8-11, the Cell Edit Chart, to find the target value for the following:
a. Use the average RBC count calculated in step 4 above to determine the Edit Ratio Percentage that coincides with the calculated average.
NOTE: Example: A count of 4 million intersects the curve at an edit ratio of 28.2%.
6.
Use the edit ratio calculated in step 5a to perform the following procedure:
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a. From the MAIN Menu, press [DIAGNOSTICS] followed by [RAW DATA].
b. Observe the RBC RER displayed on the screen from the last cycle. It should be
within +/- 1% of the calculated value from step 4a.
Using Figure 8-11 as an example, an edit ratio of 27% can be between 26 and 28 percent.
NOTE: Clockwise adjustment of R72 increases the percentage.
c. If the RBC Editing Ratio is out-of-range, adjust R72. Return to the MAIN Menu and
run another cycle. Observe the RER in the RAW DATA screen. If the ratio is still
outside of specification, repeat this process until the RBC Editing Ratio is within +/1% of your calculated value.
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d. Return to the MAIN Menu and press [RUN] to access the RUN screen. Observe the
RBC Histogram. The trailing edge should be straight with almost no humps. Refer to
Figure 8-12.
PLT Offset
1.
Connect a DVM to TP7 (PLT OUT) and ground to TP9.
2.
Adjust R16 (PLT ZERO ADJUST) so that the baseline of the noise signal is positioned at
0.00 volts +/- 0.002 volts.
PLT Gain
1.
If in the Gain Adjust mode, prepare a PLT latex dilution by following the steps below:
a. From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice.
Place a clean container under the Sample Probe and press [10 mL DISPENSE] four
times to dispense 20 mL of diluent (must press twice to dispense 10 mL).
b. Add one (1) drop of well-mixed 3.31 latex particle solution and mix well.
c. Hold the diluted latex solution under the Sample Probe and press [1/50 DILUTION] to
dispense a second dilution.
d. Place a clean container under the Sample Probe and press [1/50 DILUTION] to
aspirate the second dilution.
e. Pull open solenoid 2-5 to drain the existing solution in the RBC Mixing Chamber.
f. Pour the second latex solution into RBC Mixing Chamber.
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g. Go to step 3.
2.
If not in the Gain Adjust mode, do the following:
a. Pour 10 mL of diluent into the Pre-Mixing Cup (do not add any latex particle solution).
b. In the RUN screen, press [SPECIMEN TYPE] then the # key to active the Gain
Adjust mode. The message <PREPARING FOR GAIN ADJUST.> is displayed and
diluent in the Pre-Mixing Cup is transferred to the WBC Mixing Chamber. When the
preparation cycle is complete, the RUN screen is again displayed.
NOTE: When running PLT counts, the lower front cover must be on the instrument to prevent noise
from interfering with PLT results.
3.
Press the Touch Plate to run a cycle.
NOTE: The PLT count should be between 200 and 700. If the count is outside this range, adjust the
dilution ratio of the latex particles by adding more dilution or latex.
4.
From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE].
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5. Press [SMOOTHING OFF]. The key changes to [SMOOTHING ON] and is highlighted,
indicating the smoothing function is ON. Press [PLT HISTOGRAM]. Observe the lower
part of the histogram data to determine the channel number for peak of 100. If the gain
is adjusted properly, the peak count of 100 will be in channel 136 +/- 1 channel. Refer to
Figure 8-13.
NOTE: Turning R15 clockwise will increase the channel number; turning R15 counterclockwise will decrease the channel number.
6.
If the peak count of 100 is within specification, this procedure is completed.
7.
If the peak count of 100 is not within specification, adjust R15. Return to the RUN
screen and run another cycle. Repeat steps 4 and 5.
8.
To exit Gain Adjust mode, go to the RUN Menu and press [SPECIMEN TYPE] followed
by [PATIENT SPECIMEN].
9.
Press [MAIN] to return to the MAIN Menu. Press [SPECIAL PROTOCOLS] followed by
[REAGENT PRIME].
8.14 DILUENT AND SAMPLE VERIFICATION/ADJUSTMENT
To minimize problems like coincidence passage, the CELL-DYN 1700 uses two different dilution
ratios of whole blood to diluent. The ratio for WBC/HGB is 1:285; the ratio for RBC/MCV/PLT is
1:12,801. The following procedure will be used to verify the diluent dispense to maintain proper dilution ratios and thereby optimize instrument performance.
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Diluent Volume Verification
ALIGNMENT AND VERIFICATION
Follow the steps below for verifying diluent volume:
1.
From the MAIN Menu, press [SPECIAL PROTOCOLS] followed by [MORE] twice.
2.
Place an empty 10 mL graduated cylinder under the Sample Probe and press [10 mL
DISPENSE] twice to dispense 10 mL of diluent.
3.
Verify a volume of 10 mL +/- 0.2 mL.
4.
Place an empty 10 mL graduated cylinder under Sample Probe and press [1/50
DILUTION]. Once the probe has returned to the aspirate position, press [1/50
DISPENSE] to dispense.
5.
Verify a volume of 5 mL +/- 0.1 mL.
NOTE: The volume dispensed is under direct computer control. If the volume is out-of-range, the Dispenser and Stepper Motor drive circuitry must be repaired.
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Sample Volume Verification
ALIGNMENT AND VERIFICATION
WARNING: Potential Biohazard. Consider all specimens
potentially infectious. Wear gloves, lab coats, and safety glasses
and follow other biosafety procedures as specified in the OSHA
Bloodborne Rule (29 CFR 1910.1030) or other equivalent biosafety procedures. Also, the sample probe is sharp and potentially contaminated with infectious material. Avoid any contact
with the probe.
1.
Remove the 1/32” silicone tubing attached to the top of the Sample Probe.
2.
Attach a 100 microliter pipette to the silicone tubing.
3.
Place the tip of the pipette on the bottom of a small container and press [10 mL
DISPENSE] twice.
NOTE: Keep the tip of the pipette submerged when dispensing.
4.
Take the pipette out of the container and wipe any drop from the end of the pipette,
being careful not to wick any liquid from the end.
5.
Press [1/50 DILUTION].
6.
Verify that the column of liquid is no more than 1/16 inch above or below 100 microliter
mark on the pipette.
7.
Replace the 100 microliter pipette with a 40 microliter pipette.
8.
Place the tip of the 40 microliter pipette on the bottom of a small container and press [1/
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50 DISPENSE].
9.
Take the pipette out of the container and wipe any drop from the end of the pipette,
being careful not to wick any liquid from the end.
10. Press [1/250 DILUTION].
11. Verify that the column of liquid is no more than 1/16“ above or below 40 microliter mark
on the pipette.
12. Place a waste container under the tip of the pipette and press [1/250 DISPENSE].
13. Remove the pipette and re-attach the silicone tubing to the Sample Probe.
NOTE: The volume aspirated is under direct computer control. If the volume is out-of-range, the Sample Syringe and Stepper Motor drive must be repaired.
8.15 SAMPLE PROBE ALIGNMENT PROCEDURES
WARNING: Potential Biohazard. The sample probe
is sharp and potentially contaminated with infectious
material. Avoid any contact with the probe.
The following procedures provide step-by-step instructions to correctly adjust the positions of
Microswitches 1 through 4 on the probe assembly and to correctly align the Sample Probe height.
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If a complete alignment is to be done, the procedures should be performed in the following order:
1.
Stepper Power Test and Verification
2.
Lower Microswitch #1 Adjustment
3.
Upper Microswitch #2 Adjustment
4.
Left Microswitch #3 Adjustment
5.
Right Microswitch #4 Adjustment
6.
Sample Probe Height Adjustment
If the procedures are performed sequentially, the instrument need not be "Initialized" after each procedure.
The procedures can also be used for verification of position. When performing verification, skip all
steps calling for loosening screws and moving assemblies.
Stepper Motor Power Test and Verification
1.
1.From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times. Then
press [SERVICE DEC CODE].
2.
2.
Type in "128" from the keyboard and press Enter.
3.
3.
The test will run for approximately 45 seconds.
4.
4.
When the test is complete, the results will be displayed on the screen.
5.
5.
Compare the results displayed on the screen with the nominal values
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listed in Table 5-3 in Section 5 of this manual. Ensure that all values fall within the
specified ranges.
NOTE: Sample Probe motors B (#2) and C (#3) should be within specifications before continuing this
procedure.
Lower Microswitch #1 Adjustment
1.
Locate connector J20 on the CDM board.
2.
Slide the connector back to slightly expose pins.
3.
Connect the DVM leads to pins 2 & 3 (orange/red wires).
4.
Ensure the cable is still in contact by toggling the switch off/on. (Deactivated = 5.00 V;
Activated = 0.00 V.)
5.
From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times. Then
press [SERVICE DEC CODE]. Type in “129” and press Enter.
NOTE: Screen will give a prompt for each entry. The Enter key must be pressed after each number
is entered.
6.
Select motor 2 and press Enter. Set "Run" to 1 and press Enter. Set "Idle" to 1 and
press Enter.
7.
Press Enter to exit test procedure.
8.
Ignore the <INITIALIZE> message, press [SERVICE DEC CODE], type in “130”, and
press Enter.
9.
Select motor 2, set direction to 0, set speed to 6, and move up 800 steps.
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10. Select motor 2, set direction to 0, set speed to 8, and move up 40 steps.
11. Loosen both Locking Screws on Microswitch Assembly #1 then move the assembly to
the lowest position.
12. Select motor 2, set direction to 1, set speed to 6, and move down 813 steps.
13. Move Microswitch Assembly up until it just activates.
14. Level Microswitch Assembly, ensure switch is activated, and tighten Locking Screws.
15. Select motor 2, set direction to 0, set speed to 8, and move up 8 steps.
16. Verify that the switch is de-activated. If not, select motor 2, set direction to 1, set speed
to 8, move down 8 steps, and re-adjust the switch position as described in step 13.
17. Repeat steps 13 through 16 until switch is activated in step 13 and de-activated in step
16.
18. Select motor 2, set direction to 1, set speed to 8, and move down 23 steps.
19. Remove the DVM from connector J20. Press Enter to exit this test procedure.
20. Re-initialize the instrument by turning power OFF then ON.
Upper Microswitch #2 Adjustment
1.
Locate connector J21 on the CDM board.
2.
Slide the connector back to slightly expose pins.
3.
Connect DVM leads to pins 2 & 3 (orange/red wires).
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4. Ensure the cable is still in contact by toggling switch off/on. (Deactivated = 5.00 V;
Activated = 0.00 V.)
5.
From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times. Then
press [SERVICE DEC CODE]. Type in “129” and press Enter.
6.
Select motor 2 and press Enter. Set "Run" to 1 and press Enter. Set "Idle" to 1 and
press Enter.
7.
Press Enter to exit test procedure.
8.
Ignore the <INITIALIZE> message and press [SERVICE DEC CODE]. Type in “130”
and press Enter.
NOTE: The screen will prompt for each entry. The Enter key must be pressed after each number is
entered.
9.
Loosen both Locking Screws on Microswitch Assembly #2 and move the assembly to the
highest position and secure.
10. Select motor 2, set direction to 0, set speed to 6, and move up 800 steps.
11. Select motor 2, set direction to 0, set speed to 8, and move up 40 steps.
12. Select motor 2, set direction to 1, set speed to 8, and move down 15 steps.
13. Loosen both Locking Screws on Microswitch Assembly #2 and move the assembly down
until it just activates.
14. Level Microswitch Assembly #2, ensure the switch is activated, and tighten Locking
Screws.
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15. Select motor 2, set direction to 1, set speed to 8, and move down 8 steps.
16. Verify that the switch is de-activated. If not, select motor 2, set direction to 0, set speed
to 8, move up 8 steps and re-adjust switch position as described in step 13.
17. Repeat steps 13 through 16 until switch is activated in step 13 and de-activated in step
16.
18. Select motor 2, set direction to 1, set speed to 6, and move down 805 steps.
19. Remove the DVM from connector J21. Press Enter to exit this test procedure.
20. Re-initialize instrument by turning power OFF then ON.
Left Microswitch #3 Adjustment
1.
Locate connector J22 on the CDM board.
2.
Slide the connector back to slightly expose pins.
3.
Connect DVM leads to pins 2 & 3 (orange/red wires).
4.
Ensure the cable is still in contact by toggling switch off/on. (Deactivated = 5.00 V;
Activated = 0.00 V.)
5.
From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times. Then
press [SERVICE DEC CODE]. Type in “129” and press Enter.
NOTE: The screen will prompt for each entry. The Enter key must be pressed after each number is
entered.
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6. Select motor 3 and press Enter. Set "Run" to 1 and press Enter. Set "Idle" to 1 and
press Enter.
7.
Press Enter to exit test procedure.
8.
Ignore the <INITIALIZE> message and press [SERVICE DEC CODE]. Type in “130”
and press Enter.
9.
Select motor 2, set direction to 0, set speed to 6 and move up 826 steps.
10. Loosen both Locking Screws on Microswitch Assembly #3 and move the assembly to
rearmost position.
11. Select motor 3, set direction to 0, set speed to 6 and move CCW 245 steps.
12. Select motor 3, set direction to 0, set speed to 8, and move CCW 12 steps.
13. Select motor 3, set direction to 1, set speed to 6, and move CW 240 steps.
14. Move Microswitch Assembly forward until it just activates.
15. Level Microswitch Assembly, ensure switch is activated, and tighten Locking Screws.
16. Select motor 3, set direction to 0, set speed to 8, and move CCW 2 steps.
17. Verify the switch is de-activated. If not, select motor 3, set direction to 1, set speed to 8,
move CW 2 steps, and re-adjust switch position as described in step 14.
18. Repeat steps 14 through 17 until switch is activated in step 14 and de-activated in step
17.
19. Select motor 3, set direction to 0, set speed to 6, and move CCW 113 steps.
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20. Select motor 2, set direction to 1, set speed to 6, and move down 826 steps.
21. Remove the DVM from connector J22. Press Enter to exit this test procedure.
22. Re-initialize the instrument by turning power OFF then ON.
Right Microswitch #4 Adjustment
1.
Locate connector J23 on the CDM board.
2.
Slide the connector back to slightly expose pins.
3.
Connect the DVM leads to pins 2 & 3 (orange/red wires).
4.
Ensure cable is still in contact by toggling switch off/on.
(Deactivated = 5.00 V; Activated = 0.00 V.)
5.
From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times. Then
press [SERVICE DEC CODE]. Type in “129” and press Enter.
NOTE: The screen will prompt for each entry. The Enter key must be pressed after each number is
entered.
6.
Select motor 3 and press Enter. Set "Run" to 1 and press Enter. Set "Idle" to 1 and
press Enter.
7.
Press Enter to exit test procedure.
9.
Ignore the <INITIALIZE> message and press [SERVICE DEC CODE], type in “130” and
press Enter.
10. Select motor 2, set direction to 0, set speed to 6, and move up 826 steps.
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11. Loosen both Locking Screws on Microswitch Assembly #4 and move the assembly to
rearmost position.
12. Select motor 3, set direction to 0, set speed to 6, and move CCW 245 steps.
13. Select motor 3, set direction to 0, set speed to 8, and move CCW 12 steps.
14. Select motor 3, set direction to 1, set speed to 8, and move CW 6 steps.
15. Move the Microswitch Assembly forward until it just activates.
16. Level Microswitch Assembly, ensure the switch is activated, and tighten the Locking
Screws.
17. Select motor 3, set direction to 1, set speed to 8, and move CW 2 steps.
18. Verify switch is de-activated. If not, select motor 3, set direction to 0, set speed to 8,
move CCW 2 steps, and readjust switch position as described in step 14.
19. Repeat steps 14 through 17 until switch is activated in step 14 and de-activated in step
17.
20. Select motor 3, set direction to 1, set speed to 6, and move CW 117 steps.
21. Select motor 2, set direction to 1, set speed to 6, and move down 826 steps.
22. Remove the DVM from connector J23. Press Enter to exit this test procedure.
23. Re-initialize the instrument by turning the power OFF then ON.
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8.16 DILUENT SYRINGE CALIBRATION BLOCK ADJUSTMENT
If the diluent syringe needs to be replaced, the calibration block must be removed from the old syringe
and placed on the new syringe. Follow the instructions below for removing and installing the calibration block on the syringe. Figure 8-14 illustrates the diluent syringe.
NOTE: Refer to Section 9, Subsection: Diluent Syringe Cleaning in the CELL-DYN 1700 Operations Manual for detailed instructions on removing and replacing the diluent syringe.
1.
Remove the diluent syringe from the instrument as described in the CELL-DYN 1700
Operations Manual.
2.
Remove the calibration block from the old syringe using a 7/64” Allen wrench.
3.
Install the new syringe in its luer lock fitting.
4.
Slide the calibration block onto the plunger rod of the new syringe, then press [SYRINGE
UP] to move the drive ring up. The calibration block should be resting on top of the ring.
5.
Reinstall the front section of the syringe holding clamp. Secure it with the clamp nuts
removed during the syringe removal procedure (refer to step 1). Install the clamp nuts
with the larger hole facing the screw. Tighten the clamp nuts finger-tight with the
beveled edge toward the holding clamp. Do not overtighten.
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6. Adjust the position of the plunger so that the white plunger head is slightly below the top
of the glass barrel (leave a gap of 1 division, marked on the syringe barrel, or
approximately 200
7.
With the calibration block resting on the ring, tighten the Allen screw so that the
calibration block is firmly secured to the rod.
8.
Install the drive nut with the spacer (long narrow end) facing up. Tighten the drive nut to
finger-tight while holding the calibration block. Do not over-tighten.
9.
Press [RESTORE SYRINGE].
NOTE: Bubbles may be present during the first filling. If they do not disappear, press [MORE] twice,
then press [REAGENT PRIME] to clear the bubbles.
10. Press [MAIN] to return to the MAIN Menu.
11. Press [RUN] followed by [SPECIMEN TYPE] and [NORMAL BACKGROUND]. Using
the Touch Plate, run two to three background counts. Watch the syringe action to make
sure it fills and dispenses completely. Run background counts until acceptable results
are obtained for all background parameters.
12. Confirm calibration by running controls before running patient samples.
13. Record this procedure in the laboratory’s maintenance log.
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8.17 SHORT SAMPLE SENSOR (1700CS) ADJUSTMENT
ALIGNMENT AND VERIFICATION
Perform the following procedure to adjust the Short Sample Sensor on the Closed Sample Assembly:
1.
Obtain a new vial of CELL-DYN 16 Low Control.
2.
Using CELL-DYN diluent, dilute the Low Control to obtain a reading of 0.8 M/L +/- 0.08
for RBC on the Final Function Test reference instrument.
NOTE: Always use CELL-DYN diluent for sample dilution and always use the diluted solution the same
day. Do not allow the diluted solution to sit overnight.
3.
Hook the positive probe of the DVM to TP4 and the negative probe of the DVM to TP3
(ground of the Blood Sensor printed circuit board #9601165 located inside the Closed
Sample Assembly).
4.
From the MAIN Menu, press [DIAGNOSTICS] followed by [MORE] three times then
[SERVICE DEC CODE]. The message <ENTER NUMBER (CURRENTLY, XXX):> will
be displayed on the screen.
5.
Place a well-mixed VACUTAINER® of sample in the tube holder well (cap facing down)
and snap the tube in place. Type “33” and press Enter.
6.
When the flow sequence is completed, adjust R1 and set the voltage of TP4 to 2.5V +/0.1V.
7.
Measure the voltage of TP1.
8.
If the voltage is within the range of 0.3V - 3.25V, press [SERVICE DEC CODE] again.
The message <ENTER NUMBER (CURRENTLY, 33):> will be displayed on the screen.
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Type “34” and press Enter. The instrument will return to normal. This completes the
Closed Mode Short Sample Sensor Adjustment procedure.
If the voltage is outside the range of 0.3V - 3.25V, do the following:
a. Press [SERVICE DEC CODE]. The message <ENTER NUMBER (CURRENTLY, 33):>
will be displayed on the screen. Type “34” and press Enter.
b. Replace the black sensor block.
c. Press [SERVICE DEC CODE], type “33” and press Enter.
d. Repeat steps 6 through 9 until the voltage is within range.
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Section A.
PLANNED MAINTENANCE
SECTION OVERVIEW
The Planned Maintenance Checklist for the CELL-DYN 1700 is available to the Field Service Representative on the lap-top computer assigned to the representative and is also available from the Technical Services Group.
Future revisions of the Planned Maintenance Checklist will be released as Technical Service Bulletins.
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A-1
Appendix B
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AppendixB.
CLOSED SAMPLE OPTION
Table of Contents
B.1 SECTION OVERVIEW
B.2 CLOSED SAMPLE DESCRIPTION
Detection of Closed Sample Capability
Detection of Short Sample (Insufficient Aspiration)
Setting Open, Closed, and Pre-Dilute Calibration Factors
Using Count Test Function in the Closed Mode
Auto Clean Function
Clean Sampler Function
B.3 CLOSED SAMPLE MODULE CONFIGURATION
B.4 CLOSED SAMPLE ASSEMBLY FUNCTIONAL SEQUENCE DESCRIPTION
B.5 CLOSED SAMPLE MODULE TROUBLESHOOTING
Service DEC Codes
Motor Power Test (Service DEC Code 128)
Motor Exercise Test (Service DEC Code 130)
List of Figures
Figure 1 Ready Mode
Figure 2 VACUTAINER® Vent
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Appendix B
Figure 3 VACUTAINER® Aspiration
Figure 4 Continued VACUTAINER® Aspiration
Figure 5 VACUTAINER® Aspiration Completed
Figure 6 Sample Presentation
Figure 7 Sample Drain
Figure 8 Sample Transfer Cup Soak
Figure 9 Sample Transfer Cup Rinse
Figure 10 Sample Transfer Cup Rinse Complete
Figure 11 System Rinse
Figure 12 Diluent Tubing Air Gap
Figure 13 System Rinse Completed
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Appendix B
B.1 SECTION OVERVIEW
CLOSED SAMPLE OPTION
This appendix is designed to aid the Field Service Representative (FSR) in the troubleshooting and
repair of the CELL-DYN 1700CS Closed Sample assembly. Before attempting any repair of the
Closed Sample assembly, the FSR should verify that the instrument is operating properly in the Open
mode and that the problem is being caused by a malfunction in the Closed Sample assembly.
When a closed VACUTAINER® is inserted (cap facing down) into the VACUTAINER® holder well
and the Touch Plate on the Closed Sample Assembly is pressed, the instrument aspirates 450 µL of
sample. The Closed Sample cycle performs the following four major functions:
1.
Diluent residue rinse (from the previous cycle)
2.
Sample presentation and aspiration
3.
Sample transfer cup soak and rinse
4.
Needle waste well rinse
A detailed description of the steps in each of these functions is given in Section B.4.
B.2 CLOSED SAMPLE DESCRIPTION
Detection of Closed Sample Capability
When the instrument is turned on, the initialization of the UIC (User Interface Computer) software
determines whether the instrument is a CELL-DYN 1700 or CELL-DYN 1700CS (this differs from the
CELL-DYN 1600 which uses a code in the CCM).
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Appendix B
CLOSED SAMPLE OPTION
The instrument model is determined by a code stored in the start-up file on the hard disk. When the
software is installed at the factory or updated in the field, this code enables/disables the Closed Sample Assembly and all its ancillary support.
Detection of Short Sample (Insufficient Aspiration)
The CELL-DYN 1700CS uses a photo-detector as a short sample sensor. If the sample arrives at the
sensor too late or if an air bubble is in the line, a warning is displayed on the CRT.
Setting Open, Closed, and Pre-Dilute Calibration Factors
There are six calibration factors — WBC, RBC, HGB, MCV, PLT, and MPV — for each of the three
calibration modes, Open, Closed, and Pre-Dilute. The calibration factors for the Open mode are set
first. The Closed Mode is then calibrated against the Open mode. Calibration factors for the PreDilute mode are set separately. For specific instructions on calibrating the Open, Closed, and PreDilute modes, refer to the CELL-DYN 1700 Operations Manual.
Either the Open or Closed mode factors can be viewed by pressing the [OPEN/CLOSED FACTORS]
key in the CALIBRATION Menu. The Pre-Dilute factors can be viewed by pressing the [PREDILUTE] key in the CALIBRATION Menu.
The software automatically puts specimen results in either the Open or Pre-Dilute factors category,
depending on which method has been selected, when the operator presses the Open mode Touch
Plate. The software automatically puts specimen results in the Closed factors category when the
operator presses the Closed Sample Touch Plate.
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Appendix B
CLOSED SAMPLE OPTION
Also, the [RESET FACTORS] key allows the operator to set all the Calibration factors to 1.00 for
whichever category — Open, Closed or Pre-Dilute — is displayed on the screen. There is also a
[HELP/ERROR] key to assist the operator.
Display/Printout of Aspiration Method Used on Last Sample
The CELL-DYN 1700CS can accept blood samples in three ways: 1) Open mode, 2) Closed mode,
and 3) Pre-Dilute mode. In the RUN Menu, when sample results run in the Closed mode are displayed on the screen, the message <CLOSED> is displayed in the upper right corner after the
Sequence # field. For Pre-Diluted samples, the message <PRE-DILUTE MODE> is displayed on the
screen. No message is displayed for Open mode.
In the DATA LOG Menu or QUALITY CONTROL Menu, the letter “O”, “C” or “P” is used to designate
which method was used for each sample in the Data Log or Quality Control Log. The letter is located
in a field preceding the Date field on the right side of the display screen.
Using Count Test Function in the Closed Mode
To run a Count Test in the Closed mode, first press the [COUNT TEST] key in the DIAGNOSTICS
Menu, then press the Touch Plate on the Closed Sample Assembly.
Auto Clean Function
On the CELL-DYN 1700CS, when the [AUTO CLEAN] key in the SPECIAL PROTOCOLS Menu is
pressed, the following message is displayed on the screen:
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Appendix B
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CLOSED SAMPLE OPTION
<Please place tube containing Enzyme Cleaner in closed sampler
Then press the START CLEAN key
Otherwise press the asterisk (*) key to cancel this procedure.>
Refer to the CELL-DYN 1700 Operations Manual for instructions on performing the Auto Clean Procedure.
Clean Sampler Function
On the CELL-DYN 1700CS, when the [CLEAN SAMPLER] and [START CLEAN] keys in the SPECIAL PROTOCOLS Menu are pressed, the Closed Sample system is drained and refilled after the
tube holder well has been manually cleaned.
Refer to the CELL-DYN 1700 Operations Manual for instructions on performing the Clean Sampler
Procedure.
B.3 CLOSED SAMPLE MODULE CONFIGURATION
The Closed Sample Module includes the following major assemblies. Refer to schematic #9480081 in
Section 6.
1.
Needle Drive Assembly, Needle Drive Motor, and Stepper Driver printed circuit board
2.
Sample Pump Motor and Stepper Driver printed circuit board
3.
Diluent Pump Motor and Stepper Driver printed circuit board
4.
Sample Transfer Cup
5.
Sample Detector
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Appendix B
6. Waste Well Drain Solenoid (2-8)
7.
CLOSED SAMPLE OPTION
Sample Transfer Cup Drain Solenoid (3-8)
B.4 CLOSED SAMPLE ASSEMBLY FUNCTIONAL SEQUENCE DESCRIPTION
The following is a description of the functions performed by the Closed Sample Assembly during a
sample cycle. The sequence begins when the instrument is in the READY mode and the Touch Plate
is pressed. The sequence ends when the instrument returns to the READY state.
Legend:
The normal solid line for the tubing indicates the Closed Sample Assembly is in the READY state.
The heavy solid line for the tubing indicates the line is filled with liquid during the closed sample cycle.
The dashed line for the tubing indicates the line contains residue during the closed sample cycle.
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Appendix B
CLOSED SAMPLE OPTION
1. The instrument is in the READY state with the Cap Piercer Needle in the down position.
Diluent residue from the previous run cycle remains in the Sample Transfer Cup, Needle
Waste Well, sample line tubing, and waste line tubing. The diluent line is primed with
diluent. Refer to Figure 1.
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Appendix B
CLOSED SAMPLE OPTION
2. When the Touch Plate is pressed, the needle moves up 610 steps to pierce the
VACUTAINER® cap. The top hole in the needle is positioned inside the
VACUTAINER® and the bottom hole is positioned below the VACUTAINER® cap,
allowing the VACUTAINER® to return to atmospheric pressure. Refer to Figure 2.
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Appendix B
CLOSED SAMPLE OPTION
3. The needle then moves up another 240 steps, solenoid 3-8 opens, the Sample Pump
turns on counterclockwise to aspirate approximately 330 µL of sample from the
VACUTAINER®, then solenoid 3-8 closes. Part of the sample extends in the tubing
beyond the Sample Transfer Cup. Refer to Figure 3.
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Appendix B
CLOSED SAMPLE OPTION
4. The Sample Pump continues turning counterclockwise to aspirate another 120 µL of
sample from the VACUTAINER®, thereby displacing 120 µL of sample in the tubing
(from the first aspiration) and pushing it into the Sample Transfer Cup. The Sample
Pump then turns off. Refer to Figure 4.
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Appendix B
CLOSED SAMPLE OPTION
5. The needle moves down back through the VACUTAINER® cap to the READY position,
solenoid 3-8 opens to drain the 120 µL of sample in the Sample Transfer Cup, leaving
sample residue in the cup and waste tubing. Then solenoid 3-8 closes. The Sample
Probe moves down into the cup. Refer to Figure 5.
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Appendix B
CLOSED SAMPLE OPTION
6. The Sample Pump turns counterclockwise to transfer most of the remaining sample (190
µL) in the tubing into the Sample Transfer Cup. The Sample Probe then aspirates 30 µL
from the cup and moves back up. From this point, the instrument performs the same
sample cycle as in the Open mode. Refer to Figure 6.
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Appendix B
CLOSED SAMPLE OPTION
7. The Sample Pump turns on counterclockwise again to transfer any remaining sample in
the tubing to the Sample Transfer Cup (residue remains in the tubing). Simultaneously,
solenoid 3-8 opens to drain the remaining sample in the Sample Transfer Cup and tubing
to the waste system in preparation for the rinse cycle. Refer to Figure 7.
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Appendix B
CLOSED SAMPLE OPTION
8. The Diluent Pump turns on counterclockwise to aspirate diluent into the Sample Transfer
Cup. The diluent soaks the cup for a pre-determined duration of time. Refer to Figure 8.
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Appendix B
CLOSED SAMPLE OPTION
9. At the end of the soak cycle, solenoid 3-8 opens to rinse the diluent from the cup to the
waste system, and the Diluent Pump turns on counterclockwise to aspirate diluent into
the Sample Transfer Cup, creating a diluent rinsing action through the cup and waste
tubing. Sample residue remains in the tubing from the needle to the cup. Refer to
Figure 9.
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Appendix B
CLOSED SAMPLE OPTION
10. With solenoid 3-8 still open, the Diluent Pump turns off, allowing diluent in the Sample
Transfer Cup and tubing to be drained to the waste system. Diluent residue remains in
the tubing from the cup to solenoid 3-8. The cup rinse procedure is now completed.
Refer to Figure 10.
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Appendix B
CLOSED SAMPLE OPTION
11. Solenoid 3-8 closes and the Diluent Pump turns on counterclockwise to aspirate diluent
into the Sample Transfer Cup. Simultaneously, the Sample Pump turns clockwise to pull
diluent from the cup to backflush the needle. Solenoid 2-8 opens to drain the diluent in
the waste well (of the needle assembly) to the waste system. Refer to Figure 11.
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Appendix B
CLOSED SAMPLE OPTION
12. The Diluent Pump stops and turns clockwise slightly to create a 1/2” air gap in the tubing
by the inlet to the Sample Transfer Cup. Refer to Figure 12.
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Appendix B
CLOSED SAMPLE OPTION
13. The Sample Pump reverses direction and turns counterclockwise to transfer diluent from
the needle line to the Sample Transfer Cup. Simultaneously, solenoid 3-8 opens to drain
the diluent in the cup to the waste system This completes the Closed Sample cycle.
Refer to Figure 13.
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Appendix B
B.5 CLOSED SAMPLE MODULE TROUBLESHOOTING
CLOSED SAMPLE OPTION
Service DEC Codes
The following two Service DEC Codes are unique to the Closed Sample assembly:
1.
Service DEC Code “72” moves the sample probe into the Sample Transfer Cup, allowing
visual verification of the Sample Probe alignment in relationship to the Sample Transfer
Cup.
2.
Service DEC Code “73” moves the Sample Probe back to its READY position.
Motor Power Test (Service DEC Code 128)
The Motor Power Test can be used to test the stepper motor circuitry in the Closed Sample assembly.
The specifications for motors J, K, and L are:
LOW
MEDIUM
HIGH
PHASE
1.6 - 2.4
3.44 - 5.16
4.88 - 7.32
4.88 - 7.32
Motor Exercise Test (Service DEC Code 130)
Service DEC Code “130” can be used to exercise motors J, K, and L. The direction and speed commands for these motors are:
J/10
Needle
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Up/Pierce
Down/Withdraw
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Appendix B
K/11
L/12
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Sample
Diluent
1
CLOSED SAMPLE OPTION
0
Backflush Needle
(Clockwise)
Aspirate (Counter CW)
1
0
Air Gap (Clockwise)
Aspirate (Counter CW)
Speed command “1” moves at 50 steps per second.
Speed command “7” moves at 250 steps per second.
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Appendix C
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Appendix C.
CELL-DYN 1700 SYSTEM INTERFACE SPECIFICATION
C.1 SECTION OVERVIEW
The CELL-DYN 1700 Instrument System Interface Specification is contained in this section. It is a
separate document published by Abbot Diagnostics. It is included in this manual to assist Field Service Representatives in answering questions and resolving problems related to the interface of the
system to an external computer.
SYSTEM INTERFACE SPECIFICATION
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• CELL-DYN 1700 System
Book TOC
• TOC (Table of Contents)
CD-TOC
EXIT
Interface Specification
CELL-DYN 1700 SYSTEM
List Number 04H03-01
REVISION A
Abbott Laboratories
Abbott Park, IL 60064
04H03-01A - February 1995
©1995, Abbott Diagnostics
Abbott Diagnostics is a wholly owned
subsidiary of Abbott Laboratories
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Table of Contents
1.0 Introduction
2.0 Mechanical Interface
3.0 Electrical Interface
4.0 Data Interface
5.0 Communication Protocol
6.0 ldentification (ID) Segment
7.0 Results Segment
Table 1 Histogram Messages
Table 2 Histogram Record Dump
Table 3 Count Data Messages
Table 4 Count Record Dump
Histogram Message Example
Count Data Message Example
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1.0 Introduction
1.1
Purpose
This document describes the interfacing characteristics of the Abbott CELL-DYN® 1700
automated hematology analyzer when attached to a Host.
1.2
Definitions and Conventions
Host: external computer or data collection system.
CELL-DYN 1700: Abbott CELL-DYN 1700 or 1700CS.
This specification follows guidelines adopted at the Biomedical Instrumentation Interface
Standards Conference held at the University of Florida in December 1980 and at the
University of Texas in Dallas, April 1981.
Signal designators and related characteristics follow EIA Standard RS232C as summarized in "The Handbook of Computers and Computing," Seidman and Flores, Van Nostrad Reinhold, 1984; and "Integrated Circuits Applications Handbook," Arthur H.
Seidman, John Wiley & Sons, 1983.
Numeric equivalents of characters are shown as hexadecimal values.
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2.0 Mechanical Interface
2.1
Connector arrangement
The CELL-DYN 1700 provides a standard DB-9 male connector, labeled COM1,
mounted on the side of the instrument.
Pins on the DB-9 connector: (standard RS-232 9 pin assignments)
Pin 1:
Data Carrier Detect (DCD input) (monitored)
Pin 2:
Data to CELL-DYN 1700 (RD - receive data) (ACK/NAK, XNO/XOFF)
Pin 3:
Data to Host (LIS, results) (TD - transmit data)
Pin 4:
Data Terminal Ready (DTR) (set true)
Pin 5:
Signal Ground
Pin 6:
Data Set Ready (DSR) (monitored)
Pin 7:
Request to Send (RTS output) (set true)
Pin 8:
Clear to Send (CTS input) (monitored, if requested in Setup)
Pin 9:
Ring Indicate (RI input) (unused)
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3.0 Electrical Interface
3.1
Voltage levels and electrical characteristics are as defined by the EIA RS-232C specification.
3.2
The maximum recommended cable length is 30 meters, or 100 feet. The actual maximum workable cable length is dependent on the environment of the site, the selected
baud rate, and the equipment being connected together.
4.0 Data Interface
The system can be configured for data transmission using the Computer Setup menu. The configurable transmission parameters include data bits, stop bits, parity and baud rate.
4.1
The asynchronous method of data transmission (serial by bit) is used.
4.2
All information transmitted is in character form and is represented by 7-bit ASCII.
4.3
Transmitted characters consist of one (1) start bit, seven (7) or eight (8) data bits (least
significant first), one or no parity bit, and one (1) or two (2) stop bits.
4.4
Parity may be selected as none, odd, or even.
4.5
The transmission speed may be selected from 1200, 2400, 4800, or 9600 bits per second (bps).
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4.6
With the exception of the control characters mentioned in Section 5.2, only printable
ASCII characters (hex 20 to hex 7E) are used in a message.
5.0 Communication Protocol
5.1
5.1.1
Communication Modes
RUN Menu Automatic Transmit Mode
The User may select the Automatic Transmit mode on the Computer Setup Menu.
This mode allows the automatic transmission of results during the RUN cycle. If Automatic Transmit mode has been selected, the user may also choose whether or not to
transmit histograms along with the count data.
5.1.2
DATALOG Menu Transmit Mode
On the DATALOG Menu, the user may select results from a single sample or from
multiple samples for transmission. Only count data may be transmitted from the Data
Log menu.
5.1.3
DISPLAY SPECIMEN Menu Transmit Mode
On the DISPLAY SPECIMEN Menu, the user may request transmission of results
from the specimen being displayed. Count data are transmitted. Histograms are also
transmitted if the Automatic Transmit mode is selected in the COMPUTER SETUP
Menu.
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5.2
Transmission Control
Transmission control is provided in two ways: 1) XOFF/XON protocol in which the Host
transmits an XOFF character (hex 13) to stop transmission from the System and an
XON character (hex 11) to re-start transmission; and 2) CTS (Clear To Send) hardware
control.
The XOFF/XON protocol has a 1.5 second time-out. If no XON is received for 1.5 seconds after an XOFF, then transmission resumes anyway.
The CTS hardware control has no time-out. That is, no data transmission will be started
with CTS false. However, the transmission will still time-out if at any one time CTS
remains false for longer than the time-out selected in the COMPUTER SETUP Menu
(see Section 5.3.1 below). This is a different use and meaning for time-out, but it is
needed to prevent the Host from indefinitely delaying the CELL-DYN 1700 from processing the next sample. Re-transmission requests are also supported and discussed
in Section 5.3.
5.3
5.3.1
Response from Host
Between-Transmission Time-out
The time-out interval after transmission of one message is programmable in the
COMPUTER SETUP Menu from 100 milliseconds to 9.9 seconds in 100 millisecond
increments. Note: The timer starts just as the ETX, which ends the message, is transmitted.
5.3.2
Release for Next Message
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The Host can release the CELL-DYN 1700 to send the next message by sending an
ACK (06 hex) before the time-out interval passes. Otherwise, the CELL-DYN 1700
will begin transmission of the next message at the end of the time-out interval in 5.3.~
above.
5.3.3
Re-transmission
If for any reason the Host requires re-transmission of the message, it signals the
CELL-DYN 1700 by sending a NAK (15 hex) before expiration of the time-out. A message will be sent by the CELL-DYN 1700 a maximum of three (3) times. After that, the
same conditions prevail as after a time-out. The Host may (it is desirable but not mandatory) acknowledge the retransmission with ACK or NAK. If ACK is sent, the CELLDYN 1700 will immediately start processing the next record (if any). If NAK is sent,
the record will NOT be transmitted a third time. Receipt of either the ACK or NAK will
allow the CELL-DYN 1700 to move to the next record more quickly.
5.4
Message Format
The message format consists of the following elements:
STX, ID SEGMENT, RESULT SEGMENT, CHECK SUM, ETX.
5.4.1
There are four (4) types of messages, each distinguished by its ID segment:
WBC Histogram Message
RBC Histogram Message
PLT Histogram Message
Count Data Message
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5.4.2
The four messages taken together represent all the results of testing a single specimen.
5.4.3
Each message begins with STX (hex 02).
5.4.4
The Identification (ID) segment is described fully in Section 6.
5.4.5
The Result segment is described fully in Section 7.
5.4.6
The Check Sum is always provided and may be optionally processed by the Host to
verify correct transmission. It is generated by taking the module-256 sum of all the
characters in the message except the STX and ETX characters. The two-digit hexadecimal representation of the Check Sum byte is placed immediately before the ETX
as two ASCII characters.
5.4.7
The ETX character (hex 03) is the last character of the message.
5.4.8
Message Length
Each of the three Histogram messages is 1204 characters long. The Count Data
message is 406 characters long. These message lengths count all characters from
STX to ETX inclusive.
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5.4.9 Data Representation
Numeric data are transmitted in fields of fixed length with zeros used to fill empty
spaces on the left. Out-of-range numeric values are represented by strings of ">"
characters (hex 3E), and undefined numeric values are represented by strings of "-"
characters (hex 2D). Alphanumeric data are transmitted in fields of fixed length
enclosed in double quotation marks (hex 22). Within the quotation marks, the data
are right-justified and blanks (hex 20) are used to fill empty spaces. Fields are separated by commas (hex 2C).
6.0 Identification (ID) Segment
6.0.1 For counting purposes, the STX mentioned in Section 5.4.3 is taken as byte 1.
6.1
The Identification segment of each message identifies the type of message and the
specimen the message represents.
6.2
Message Type - Field 1
The Message Type field identifies the message. There are four message types:
WBC Histogram Message - ...................Type "WBC"
RBC Histogram Message- .....................Type "RBC"
PLT Histogram Message - Type "PLT"
Count Data Message Type " " (3 blanks)
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6.3
Instrument Type - Field 2
The Instrument Type is an alphanumeric field of seven (7) characters enclosed in double quotation marks. The string for the CELL-DYN 1700CS is sent as "CD1700C" and
for the CELL-DYN 1700 as " CD1700".
6.4
Serial Number- Field 3
The Instrument Serial Number is an alphanumeric field of twelve (12) characters
enclosed in double quotation marks. It is not available in this release, so the field is
transmitted as "------------" (twelve dashes).
6.5
Sequence Number - Field 4
The Sequence Number is a numeric field of four (4) characters with a value ranging from
O to 4999.
6.6
Specimen Type - Field 5
The Specimen Type is the fifth field in the Identification Segment. It occupies a numeric
field of two characters whose value identifies the specimen type as follows:
00:
01:
02:
03:
Patient type
Replicate 1
Replicate 2
Replicate 3
Interface Specification CELL-DYM® 1700 System
"PATIENT"
"REPLIC 1"
"REPLIC 2"
"REPLIC 3"
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04:
05:
06:
07:
08:
09:
10:
11:
12:
13:
14:
15:
16:
17:
6.7
Replicate 4
Replicate 5
Replicate 6
Replicate 7
Replicate 8
Replicate 9
Low Control
Normal Control
High Control
Background
Electrical Background
Calibrator
Gain Adjustment
Auto Calibration
"REPLIC 4"
"REPLIC 5"
"REPLIC 6"
"REPLIC 7"
"REPLIC 8"
"REPLIC 9"
"LOW CTRL"
"NOR CTRL"
"HI CTRL"
" BACKGRD"
"ELECBKGD"
"CALIBRATR"
"GAIN ADJ"
"AUTO GAL"
Operator ID - Field 6
The Operator ID is a alphanumeric field of three (3) digits enclosed in double quotation
marks. If no Operator ID is specified, the field is transmitted as "---".
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6.8
Specimen Date - Field 7
The Specimen Date, giving the date on which the specimen was run, is an alphanumeric
field of eight (8) characters enclosed in double quotation marks. The default format of
the date is MM/DD/YY, where MM represents the month in two digits, DD represents the
day of the month, and YY represents the year. The date format can be changed in the
DATE/TIME Menu.
6.9
Specimen Time - Field 8
The Specimen Time is a alphanumeric field of five (5) characters enclosed in double
quotation marks. It gives the time at which the specimen was run in standard 24-hour
format.
6.10
Specimen ID- Field 9
The Specimen ID is an alphanumeric field of nine (9) characters enclosed in double
quotation marks. If the specimen is a patient specimen, the Specimen ID is entered by
the operator. Otherwise, the Specimen ID is generated by the CELL-DYN 1700 to identify special types of samples, such as controls. If not specified, the Specimen ID is transmitted as "---------" (9 dashes). See Section 6.6 (Field 5).
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6.11
Specimen Name- Field 10
The Specimen Name is the tenth field in the Identification Segment. It occupies a text
field of sixteen (16) characters enclosed in double quotation marks, and it is undefined
except for patient samples.
6.12
Specimen Sex - Field 11
The Specimen Sex field consists of one (1) character "M" or "F" enclosed in double quotation marks. If not entered by the operator, Specimen Sex is transmitted as a blank " ".
6.13
Specimen Date of Birth (DOB)- Field 12
The Specimen Date of Birth field has an identical format to Field 7, except that Field 7 is
automatically generated by the CELL-DYN 1700. If DOB is not entered by the operator,
it is transmitted as "bb/bb/bb" where the "bb"s are actually 2 ASCII blanks each.
6.14
Dr Name - Field 13
The Dr Name field is an alphanumeric field of twenty two (22) characters enclosed in
double quotation marks.
Interface Specification CELL-DYM® 1700 System
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13
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6.15
Collection Date - Field 14
The Collection Date field is an alphanumeric field of five (5) characters enclosed in double quotation marks. The format is MM/DD, where MM represents the month in two digits and DD represents the day. The year is not included. It gives the date at which the
specimen was collected. If the Collection Date is not entered, it is transmitted as "bb/bb"
where the "bb"s are 2 ASCII blanks as in Field 12 above.
6.16
Collection Time - Field 15
The Collection Time field is an alphanumeric field of five (5) characters enclosed in double quotation marks. It gives the time at which the specimen was collected. If the Collection Time is not entered, it is transmitted as "bb:bb" ("bb"s are 2 ASCII blanks each and
":" is the middle byte).
6.17
Comment- Field 16
The Comment field is an alphanumeric field of sixteen (16) characters enclosed in double quotation marks.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
14
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NOTE: Fields 1 to 16 above are common in both format AND actual content for a given
sample and, as such, are transmitted multiple times when histogram transmission is
requested (first (if sent) with all three histograms and then again with the count record).
Given today's typical transmission speeds and the lengths of the rest of the fields, the
convenience of having the header information combined with each data group has been
chosen over the absolute smallest or fastest transmission. Also, in the time frame of
even normal sample processing, the transmissions (at 9600 baud) should take less than
4 seconds out of the 60 second (nominal) cycle time.
7.0 Results Segment
7.1
The results for each specimen are sent in the format described. All numeric fields are
integers, and some need to be scaled by the Host.
7.2
Histogram Messages
Refer to Table 1, Histogram Messages, and Table 2, Histogram Record Dump.
7.2.1
Scale Factor- Field 17
The Scale Factor is a numeric field of five (5) characters. It is not implemented at this
time and is therefore always transmitted as 00000 (5 ASCII zeros without the surrounding double quotes).
7.2.2
Channel Data - Fields 18 through 273
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
15
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The Channel Data fields are numeric fields of three (3) characters each, giving normalized counts for every channel of the designated histogram (WBC, RBC, or PLT).
Data for each channel will occupy 4 bytes total since there will be a comma between
each 3 digit value. Each value is rightjustified with ASCII zero "0" (not null) characters to keep the fields a fixed length. So a channel value of binary zero is transmitted
as three ASCII zero characters (000).
NOTE: A comma (,) follows the last field which immediately precedes the CRC characters.
7.3
Count Data Message
Refer to Table 3, Count Data Messages, and Table 4, Count Record Dump.
The parameters reported by the instrument may be represented in any of four different
sets of measurement units as follows:
Set 1 - Standard USA
Set 2 - SI
Set 3 - Modified SI (HGB/MCHC in mmol/L, MCH in fmol)
Set 4 - Modified SI (HCT/PCT in %)
NOTE: The numeric values transmitted to the host are always sent in Standard USA
units even if the unit of measure, also being transmitted, is Set 2, 3, or 4.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
16
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To convert the integer transmitted in the field corresponding to a particular parameter to
the correct value for that parameter in the units being used, the decimal point must be
moved leftward from its implied position to the right of the integer. The description of
each field gives a shift count indicating how many places to move the decimal point in
each case. If, for example, the decimal point is to be moved two places to the left, a field
value of 00123 becomes 1.23. An integer representing the Units Set currently in effect is
transmitted in the the Units Set field (Section 7.3.46).
For Units Set 3, the HGB, MCH, and MCHC values must be multiplied by 0.6206 after
the decimal point has been positioned.
The units of measure associated with the four sets are explained in the CELL- DYN
1700 Operations Manual.
7.3.1
WBC Count- Field 17
The WBC Count is in a numeric field five (5) characters long.
Units Set
1
2-3
4
7.3.2
Shift Count
1
1
1
Units Label
K/uL
G/L
10E9/L
Spare Field - Field 18
The Spare Field is a numeric field of five (5) characters. It has a constant value of
zero (0) at this time, but is transmitted as five "-" (dashes) without surrounding double
quotes, since it is a numeric field.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
17
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7.3.3
LYM Count - Field 19
The LYM Count is in a numeric field five (5) characters long.
Units Set
1
2-3
4
7.3.4
Shift Count
1
1
1
Units Label
K/uL
G/L
10E9/L
MID Count - Field 20
The MID Count is in a numeric field five (5) characters long.
Units Set
1
2-3
4
7.3.5
Shift Count
1
1
1
Units Label
K/uL
G/L
10E9/L
GRAN Count- Field 21
The GRAN Count is in a numeric field five (5) characters long.
Units Set
1
2-3
4
Shift Count
1
1
1
Units Label
K/uL
G/L
10E9/L
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
18
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7.3.6
Spare Field - Field 22
The Spare Field is a numeric field of five (5) characters. It has a constant value of
zero (0) at this time, but is transmitted as five "-" (dashes) without surrounding double
quotes, since it is a numeric field.
7.3.7
RBC Count - Field 23
The RBC Count is in a numeric field five (5) characters long.
Units Set
1
2-3
4
7.3.8
Shift Count
2
2
2
Units Label
M/uL
T/L
10E12/L
HGB Value - Field 24
The HGB Value is in a numeric field, five (5) characters long.
Units Set
1
2
3
4
Shift Count
1
0
1 (x 0.6206)
0
Units Label
g/dL
g/L
mmol/L
g/L
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
19
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7.3.9
HCT Value - Field 25
The HCT Value is in a numeric field five (5) characters long.
Units Set
1
2-3
4
7.3.10
Shift Count
1
3
1
Units Label
%
L/L
%
MCV Value - Field 26
The MCV Value is in a numeric field five (5) characters long.
Units Set
1-4
7.3.11
Shift Count
1
Units Label
fL
MCH Value - Field 27
The MCH Value is in a numeric field five (5) characters long.
Units Set
1-2
3
4
Shift Count
1
2 (x 0.6206)
1
Units Label
pg
fmol
pg
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
20
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7.3.12
MCHC Value - Field 28
The MCHC Value is in a numeric field five (5) characters long.
Units Set
1
2
3
4
7.3.13
Shift Count
1
0
1 (x 0.6206)
0
Units Label
g/dL
g/L
mmol/L
g/L
RDW Value - Field 29
The RDW Value is in a numeric field five (5) characters long
Units Set
1-4
7.3.14
Shift Count
1
Units Label
%
PLT Count - Field 30
The PLT Count is in a numeric field five (5) characters long.
Units Set
1
2-3
4
Shift Count
0
0
0
Units Label
K/uL
G/L
10E9/L
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
21
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7.3.15
MPV Value - Field 31
The MPV Value is in a numeric field five (5) characters long.
Units Set
1-4
7.3.16
Shift Count
1
Units Label
fL
PCT Value - Field 32
The PCT Value is in a numeric field five (5) characters long.
Units Set
1
2-3
4
7.3.17
Shift Count
2
1
2
Units Label
%
mL/L
%
PDW Value - Field 33
The PDW Value is in a numeric field five (5) characters long.
Units Set
1-4
7.3.18
Shift Count
1
Units Label
10(GSD)
Spare Field - Field 34
The Spare Field is a numeric field of five (5) characters. It has a constant value of
zero (0) at this time, but is transmitted as five "-" (dashes) without surrounding double
quotes, since it is a numeric field.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
22
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7.3.19
LYM % Value - Field 35
Field 35 The LYM % Value is in a numeric field five (5) characters long.
Units Set
1-4
7.3.20
Shift Count
1
Units Label
%
MID % Value - Field 36
The MID % Value is in a numeric field five (5) characters long.
Units Set
1-4
7.3.21
Shift Count
1
Units Label
%
GRAN % Value - Field 37
The GRAN % Value is in a numeric field five (5) characters long.
Units Set
1-4
7.3.22
Shift Count
1
Units Label
%
Spare Field - Field 38
The Spare Field is a numeric field of five (5) characters. It has a constant value of
zero (0) at this time, but is transmitted as five "-" (dashes) without the surrounding
double quotes, since it is a numeric field.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
23
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7.3.23
Spare Field - Field 39
The Spare Field is in a numeric field. It has not been implemented at this time, so it is
always transmitted as a "-" single dash without the surrounding double quotes.
7.3.24
Spare Flag - Field 40
The Spare Flag is a numeric field of one (1) character. It has a constant value of zero
(0) at this time, but is transmitted as a single "-" (dash) without the surround double
quotes, since is presumed to be a spare numeric flag.
7.3.25
R4 WBC Flag - Field 41
The R4 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the flag is set, and a value of 0 indicates that it is clear.
7.3.26
GR3 WBC Flag - Field 42
The GR3 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the GRAN R3 flag is set, and a value of 0 indicates that it is clear.
7.3.27
MR3 WBC Flag - Field 43
The MR3 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the Mono R3 WBC flag is set, and a value of 0 indicates that it is clear.
7.3.28
MR2 WBC Flag - Field 44
The MR2 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the Mono R2 WBC flag is set, and a value of 0 indicates that it is clear.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
24
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7.3.29
LR2 WBC Flag - Field 45
The LR2 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the Lym R2 WBC flag is set, and a value of 0 indicates that it is clear.
7.3.30
Rl WBC Flag - Field 46
The R1 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the Rl WBC flag is set, and a value of 0 indicates that it is clear.
7.3.31
R0 WBC Flag - Field 47
The R0 WBC Flag is in a numeric field of one character. A value of 1 indicates that
the flag is set, and a value of 0 indicates that it is clear.
7.3.32
Spare Flags - Fields 48 through 60
There are thirteen Spare Flag fields which are each a numeric field of one (1) character. It has a constant value of zero (0) at this time. These are transmitted as follows
(no surrounding quotes):
-,-,-,-,-,-,-,-,-,-,-,-,-,
7.3.33
LRI Flag - Field 61
The LRI Flag is in a numeric field of two characters. A value of 16 indicates that the
flag is set, and a value of 00 indicates that it is clear.
7.3.34
URI Flag - Field 62
The URI Flag is in a numeric field of two characters. A value of 32 indicates that the
flag is set, and a value of 00 indicates that it is clear.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
25
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7.3.35
VVBC Lower Meniscus Time - Field 63
The WBC Lower Meniscus Time is in a numeric field five (5) characters long. It gives
the time in units of milliseconds.
7.3.36
WBC Upper Meniscus Time - Field 64
The WBC Upper Meniscus Time is in a numeric field five (5) characters long. It gives
the time in units of milliseconds.
7.3.37
RBC Lower Meniscus Time - Field 65
The RBC Lower Meniscus Time is in a numeric field five (5) characters long. It gives
the time in units of milliseconds.
7.3.38
RBC Upper Meniscus Time - Field 66
The RBC Upper Meniscus Time is in a numeric field five (5) characters long. It gives
the time in units of milliseconds.
7.3.39
Recount RBC Lower Meniscus Time - Field 67
The Recount RBC Lower Meniscus Time is in a numeric field five (5) characters long.
It gives the time in units of milliseconds. It has a value of 0 if there was no recount.
7.3.40
Recount RBC Upper Meniscus Time - Field 68
The Recount RBC Upper Meniscus Time is in a numeric field five (5) characters long.
It gives the time in units of milliseconds. It has a value of 0 if there was no recount.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
26
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7.3.41
Limits Set - Field 69
The Limits Set is a numeric field of one character. It indicates which set of patient limits (1 to 4) was in effect for the given patient specimen.
7.3.42
Sample Mode - Field 70
The Sample Mode is an alphanumeric field of one character. An "O" (letter O) indicates that the specimen was taken in Open Sample mode; a "C" indicates Closed
Sample mode, and a "P" indicates Pre-Dilute mode.
7.3.43
RBC Metering Fault Flag - Field 71
The RBC Metering Fault flag is a numeric field of one character. A "0" (zero) indicates
that there was no metering fault. A "1" (one) indicates a flow error, and a "2" (two)
indicates a clog.
7.3.44
WBC Metering Fault Flag - Field 72
The VVBC Metering Fault flag is a numeric field of one character. A "0" (zero) indicates that there was no metering fault. A "1" (one) indicates a flow error, and a "2"
(two) indicates a clog.
7.3.45
Sampling Error or Other Sample Processing Fault - Field 73
This is a numeric field of one character. A "0" (zero) indicates there was no error. A
"1" (one) indicates there was an error.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
27
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7.3.46
Units Set Field - Field 74
This is a numeric field of one character. The value is 1, 2, 3, or 4 as described in Section 7.3.
There are four sets of measurement units. A problem arises under the following conditions:
1. Some data is collected and stored under one unit of measure.
2. Subsequently, data is collected and.stored under another unit of measure.
3. All the data is transmitted to a Laboratory Information System.
The numeric results data, starting in field 17, are always~ sent as Standard USA units
even though that data may be displayed in a different unit of measure on the instrument. The displayed unit of measure, sent in field 74, is transmitted for informational
purposes only and may or may not correspond to the Standard USA format of the
transmitted numeric results data.
NOTE: A comma (,) follows the last field which immediately precedes the CRC characters.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
28
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TABLE 1: HISTOGRAM MESSAGES
Field#
N/A
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
Description
STX (0x02)
Message Type
Instrument Type
Serial Number
Sequence Number
Specimen Type
Operator ID
Specimen Date
Specimen Time
Specimen ID
Specimen Name
Specimen Sex
Specimen DOE
Dr Name
Collect Date
Collect Time
Comment
The above fields are common to both this
Table and Table 3
Scale Factor (n/a)
Interface Specification CELL-DYM® 1700 System
Start at
Byte
1
2
8
18
33
38
41
47
58
66
78
97
101
112
137
145
153
Byte
Length
1
3*
7*
12 *
4
2
3*
8*
5*
9*
16 *
1*
8*
22 *
5*
5*
16 *
Paragraph
#
5.4.3
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
6.11
6.12
6.13
6.14
6.15
6.16
6.17
172
5
7.2.1
04H03-01A-February1995
29
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TABLE 1: HISTOGRAM MESSAGES
Field#
Description
18
"
273
N/A
N/A
Channel 1 Data
"
" "
Channel 256 Data
CRC (2 hex ASCII printable)
ETX (0x03)
Start at
Byte
178
"
1198
1202
1204
Byte
Length
3
"
3
2
1
Paragraph
#
7.2.2
...
7.2.2
5.4.6
5.4.7
* indicates characters enclosed in double quotation marks.
Note: If a field contains 3 characters plus double quotation marks and a comma, the
total number of bytes used is 6. See the Histogram Message Example.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
30
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TABLE 2: HISTOGRAM RECORD DUMP
02 22 57 42 43 22 2C 22-20 43 44 31 37 30 30 22
2C 22 2D 2D 2D 2D 2D 2D-2D 2D 2D 2D 2D 2D 22 2C
34 32 38 38 2C 30 30 2C-22 2D 2D 2D 22 2C 22 31
30 2F 31 39 2F 39 34 22-2C 22 31 36 3A 34 34 22
2C 22 2D 2D 2D 2D 2D 2D-2D 2D 2D 22 2C 22 20 20
20 20 20 20 20 20 20 20-20 20 20 20 20 31 22 2C
22 4D 22 2C 22 20 20 2F-20 20 2F 20 20 22 2C 22
20 20 20 20 20 20 20 20-20 20 20 20 20 20 20 20
20 20
22 20
20 20
2C 30
2C 30
2C 30
2C 30
2C 30
...
...
...
2C 30
20
20
20
30
30
30
35
33
20
3a
20
30
30
30
37
35
20 2
20 22
20 20
2C 30
2C 30
2C 30
2C 30
2C 30
22
22
20
30
30
34
35
32
2C-22
C-22
20-20
30-2C
30-2C
32-2C
39-2C
35-2C
20
20
22
30
30
30
30
30
20 2F
20 20
2C 30
30 30
30 30
34 38
35 35
32 30
20 20
20 20
30 30
2C 30
2C 30
2C 30
2C 30
2C 30
22
20
30
30
30
35
34
31
"WBC"," CD1700"
,"- - - - - - - - - - - -",
4288,00,"- - -","1
0/19/91","16:44"
,"- - - - - - - - -","
1",
"M" , " / / " , "
2C
20
30
30
30
34
35
36
" , " / ",
" : ","
",00000
,000,000,000,000
,000,000,000,000
,000,042,048,054
,057,059,055,045
,035,025,020,016
35 32 2C 30 35 33-2C 30 35 31 2C 30 35 30
,052,053,051,050
(channels have been deleted)
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
31
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TABLE 2: HISTOGRAM RECORD DUMP
2C 30 34 39 2C 30 31 38-2C 30 34 37 2C 30 30 30
2C 30 30 30 2C 30 30 30-2C 30 30 30 2C 30 30 30
2C 36 35 03
,049,048,047,000
,000,000,000,000
,65.
Note: Each line contains 16 characters starting with i, not zero. The "02" and "." bolded on the first line
refer to the STX character. The "03" and "65" bolded on the last line refer to the ETX and CRC characters respectively.
TABLE 3: COUNT DATA MESSAGE
Field#
N/A
1
2
3
4
5
6
7
8
9
10
Description
STX (0x02)
Message Type
Instrument Type
Serial Number
Sequence Number
Specimen Type
Operator ID
Specimen Date
Specimen Time
Specimen ID
Specimen Name
Interface Specification CELL-DYM® 1700 System
Start at
Byte
1
2
8
18
33
38
41
47
58
66
78
Byte
Length
1
3*
7*
12 *
4
2
3*
8*
5*
9*
16 *
04H03-01A-February1995
Paragraph
#
5.4.3
6.2
6.3
6.4
6.5
6.6
6.7
6.8
6.9
6.10
6.11
32
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TABLE 3: COUNT DATA MESSAGE
Field#
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
Description
Specimen Sex
Specimen DOE
Dr Name
Collect Date
Collect Time
Comment
The above fields are common to both this
Table and Table 1
WBC Count
Spare Field
LYM Count
MID Count
GRAN Count
Spare Field
RBC Count
HGB Value
HCT Value
MCV Value
MCH Value
MCHC Value
RDW Value
Interface Specification CELL-DYM® 1700 System
Start at
Byte
97
101
112
137
145
153
Byte
Length
1*
8*
22 *
5*
5*
16 *
Paragraph
#
6.12
6.13
6.14
6.15
6.16
6.17
172
178
184
190
196
202
208
214
220
226
232
238
244
5
5
5
5
5
5
5
5
5
5
5
5
5
7.3.1
7.3.2
7.3.3
7.3.4
7.3.5
7.3.6
7.3.7
7.3.8
7.3.9
7.3.10
7.3.11
7.3.12
7.3.13
04H03-01A-February1995
33
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TABLE 3: COUNT DATA MESSAGE
Field#
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48- 60
61
62
Description
PLT Count
MPV Value
PCT Value
PDW Value
Spare Field
LYM % Value
MID % Value
GRAN % Value
Spare Field
Spare Field
Spare Field
R4 WBC Flag
GR3 WBC Flag
MR3 WBC Flag
MR2 WBC Flag
LR2 WBC Flag
R1 WBC Flag
R0 WBC Flag
Spare Flags
LRI Flag
URI Flag
Interface Specification CELL-DYM® 1700 System
Start at
Byte
250
256
262
268
274
280
286
292
298
304
306
308
310
312
314
316
318
320
322
348
351
Byte
Length
5
5
5
5
5
5
5
5
5
1
1
1
1
1
1
1
1
1
1
2
2
04H03-01A-February1995
Paragraph
#
7.3.14
7.3.15
7.3.16
7.3.17
7.3.18
7.3.19
7.3.20
7.3.21
7.3.22
7.3.23
7.3.24
7.3.25
7.3.26
7.3.27
7.3.28
7.3.29
7.3.30
7.3.31
7.3.32
7.3.33
7.3.34
34
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TABLE 3: COUNT DATA MESSAGE
Field#
63
64
65
66
67
68
69
70
71
72
73
74
n/a
n/a
Description
WBC Lower Meniscus Time
WBC Upper Meniscus Time
RBC Lower Meniscus Time
RBC Upper Meniscus Time
Recount RBC Lower
Meniscus Time
Recount RBC Upper
Meniscus Time
Limits Set
Sample Mode
RBC Meteri Fault Flag
WBC Meteri Fault Flag
Sampling Error/incomplete
Aspiration Flag
Units Set Field
CRC (2 hex ASCII printable)
ETX (0x03)
Start at
Byte
354
360
366
372
Byte
Length
5
5
5
5
Paragraph
#
7.3.35
7.3.36
7.3.37
7.3.38
378
5
7.3.39
384
5
7.3.40
390
392
396
398
1
1*
1
1
7.3.41
7.3.42
7.3.43
7.3.44
400
1
7.3.45
402
404
406
1
2
1
7.3.46
5.4.6
5.4.7
* indicates characters enclosed in double quotation marks
Note: If a field contains 3 characters plus double quotation marks and a comma, the
total number of bytes used is 6. See the Count Data Message Example.
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
35
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TABLE 4 COUNT RECORD DUMP
Table 1:
Histogram Record Dump
02 22 20 20 20 22 2C 22-20 43 44 31 37 30 30 22
2C 22 2D 2D 2D 2D 2C 2D-2D 2D 2D 2D 2D 2D 22 2C
34 32 38 38 2C 30 30 2C-22 2D 2D 2D 22 2C 22 31
30 2F 31 39 2F 39 34 22-2C 22 31 36 3A 34 34 22
2C 22 2D 2D 2D 2D 2D 2D-2D 2D 2D 22 2C 22 20 20
20 20 20 20 20 20 20 20-20 20 20 20 20 31 22 2C
22 4D 22 2C 22 20 20 2F-20 20 2F 20 20 22 2C 22
20 20 20 20 20 20 20 20-20 20 20 20 20 20 20 20
20 20 20 20 20 20 22 2C-22 20 20 2F 20 20 22 2C
22 20 20 3A 20 20 22 2C-22 20 20 20 20 20 20 20
20 20 20 20 20 20 20 20-20 22 2C 30 30 35 32 35
2C 2D 2D 2D 2D 2D 2C 30-30 30 31 30 2C 30 30 30
31 36 2C 30 30 34 39 39-2C 2D 2D 2D 2D 2D 2C 30
30 32 35 31 2C 30 30 30-37 34 2C 30 30 32 32 36
2C 30 30 39 30 30 2C 30-30 32 39 35 2C 30 30 33
32 37 2C 30 30 31 38 37-2C 30 30 33 39 35 2C 30
30 30 36 38 2C 30 30 30-32 37 2C 30 30 31 38 38
2C 2D 2D 2D 2D 2D 2C 30-30 30 31 39 2C 30 30 30
33 31 2C 30 30 39 35 30-2C 2D 2D 2D 2D 2D 2C 2D
2C 2D 2C 30 2C 30 2C 30-2C 31 2C 31 2C 30 2C 30
Interface Specification CELL-DYM® 1700 System
. " " , " CD1700"
,"- - - - - - - - - - - -",
4288,00,"---","1
0/19/94","16:44"
,"- - - - - - - - -" , "
1",
"M" , " / / " , "
"," / ",
" : ","
",00525
,-----,00010,000
16,00499,-----,0
0251,00074,00226
,00900,00295,003
27,00187,00395,0
0068,00027,00188
,-----,00019,000
31,00950,-----,,-,0,0,0,1,1,0,0
04H03-01A-February1995
36
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Table 1:
Histogram Record Dump (Continued)
2C 2D 2C 2D 2C 2D 2C 2D-2C 2D 2C 2D 2C 2D 2C 2D
2C 2D 2C 2D 2C 2D 2C 2D-2C 2D 2C 30 30 2C 30 30
2C 30 34 38 33 37 2C 30-32 31 32 31 2C 30 36 34
31 31 2C 30 35 30 37 30-2C 30 30 30 30 30 2C 30
30 30 30 30 2C 34 2C 22-4F 22 2C 30 2C 30 2C 30
2C 31 2C 33 37 03
,-,-,-,-,-,-,-,,-,-,-,-,-,00,00
,04837,02121,064
11,05070,00000,0
0000,4,"O",0,0,0
,1,37.
HISTOGRAM MESSAGE EXAMPLE
Actual transmission
(no CR/LF)
[remarks for text here]
[STX]
[start of text]
"WBC",
[message type]
"CD1700C",
[instrument type]
"------------",
[serial #]
1040,
[sequence no.]
00,
[specimen type]
"123",
[operator ID]
"08/31/94",
[specimen date]
Interface Specification CELL-DYM® 1700 System
Field 1
Field 5
04H03-01A-February1995
37
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HISTOGRAM MESSAGE EXAMPLE (Continued)
"12:34",
[specimen time]
"123456789",
[specimen ID]
"
[specimen name]
JOHN DOE",
"M",
[specimen sex]
"10/1 5/46",
[specimen DOB
"
[doctor name]
DR JANE DOE",
Field 10
"08/30",
[collect date]
'09:50",
[collect time]
Field 15
[comment]
Field 16
00000,
[scale factor n/a]
Field 17
000,
[channel 1 data]
Field 18
000,
[channel 2 data]
000,
[channel 3 data]
000,
[channel 4 data]
000,
[channel 5 data]
000,
[channel 6 data]
"
sample 1",
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
38
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HISTOGRAM MESSAGE EXAMPLE (Continued)
000,
[channel 7 data]
000,
[channel 8 data]
000,
[channel 9 data]
000,
[channel 10 data]
000,
[channel 11 data]
000,
[channel 12 data]
000,
[channel 13 data]
000,
[channel 14 data]
000,
[channel 15 data]
000,
[channel 16 data]
000,
[channel 17 data]
000,
[channel 18 data]
000,
[channel 19 data]
000,
[channel 20 data]
000,
[channel 21 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
39
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HISTOGRAM MESSAGE EXAMPLE (Continued)
000,
[channel 22 data]
000,
[channel 23 data]
000,
[channel 24 data]
000,
[channel 25 data]
034,
[channel 26 data]
034,
[channel 27 data]
034,
[channel 28 data]
034,
[channel 29 data]
054,
[channel 30 data]
054,
[channel 31 data]
054,
[channel 32 data]
054,
[channel 33 data]
080,
[channel 34 data]
080,
[channel 35 data]
080,
[channel 36 data]
080,
[channel 37 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
40
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HISTOGRAM MESSAGE EXAMPLE (Continued)
093,
[channel 38 data]
093,
[channel 39 data]
093,
[channel 40 data]
093,
[channel 41 data]
080,
[channel 42 data]
080,
[channel 43 data]
080,
[channel 44 data]
080,
[channel 45 data]
071,
[channel 46 data]
071,
[channel 47 data]
071,
[channel 48 data]
071,
[channel 49 data]
063,
[channel 50 data]
063,
[channel 51 data]
063,
[channel 52 data]
063,
[channel 53 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
41
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HISTOGRAM MESSAGE EXAMPLE (Continued)
041,
[channel 54 data]
041,
[channel 55 data]
041,
[channel 56 data]
041,
[channel 57 data]
032,
[channel 58 data]
041,
[channel 59 data]
029,
[channel 60 data]
029,
[channel 61 data]
029,
[channel 62 data]
029,
[channel 63 data]
029,
[channel 64 data]
027,
[channel 65 data]
027,
[channel 66 data]
027,
[channel 67 data]
027,
[channel 68 data]
027,
[channel 6 9 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
42
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HISTOGRAM MESSAGE EXAMPLE (Continued)
034,
[channel 70 data]
034,
[channel 71 data]
034,
[channel 72 data]
034,
[channel 73 data]
034,
[channel 74 data]
039,
[channel 75 data]
039,
[channel 76 data]
039,
[channel 77 data]
039,
[channel 78 data]
037,
[channel 79 data]
037,
[channel 80 data]
037,
[channel 81 data]
037,
[channel 82 data]
037,
[channel 83 data]
037,
[channel 84 data]
037,
[channel 85 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
43
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HISTOGRAM MESSAGE EXAMPLE (Continued)
041,
[channel 86 data]
041,
[channel 87 data]
041,
[channel 88 data]
041,
[channel 89 data]
046,
[channel 90 data]
046,
[channel 91 data]
046,
[channel 92 data]
046,
[channel 93 data]
046,
[channel 94 data]
046,
[channel 95 data]
046,
[channel 96 data]
046,
[channel 97 data]
046,
[channel 98 data]
046,
[channel 99 data]
046,
[channel 100 data]
046,
[channel 101 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
44
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HISTOGRAM MESSAGE EXAMPLE (Continued)
046,
[channel 102 data]
046,
[channel 103 data]
046,
[channel 104 data]
046,
[channel 105 data]
054,
[channel 106 data]
054,
[channel 107 data]
054,
[channel 108 data]
054,
[channel 109 data]
058,
[channel 110 data]
054,
[channel 111 data]
054,
[channel 112 data]
054,
[channel 113 data]
063,
[channel 114 data]
063,
[channel 115 data]
063,
[channel 116 data]
063,
[channel 117 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
45
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HISTOGRAM MESSAGE EXAMPLE (Continued)
066,
[channel 118 data]
063,
[channel 119 data]
063,
[channel 120 data]
063,
[channel 121 data]
063,
[channel 122 data]
066,
[channel 123 data]
066,
[channel 124 data]
066,
[channel 125 data]
066,
[channel 126 data]
066,
[channel 127 data]
066,
[channel 128 data]
066,
[channel 129 data]
066,
[channel 130 data]
068,
[channel 131 data]
068,
[channel 132 data]
068,
[channel 133 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
46
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HISTOGRAM MESSAGE EXAMPLE (Continued)
068,
[channel 134 data]
073,
[channel 135 data]
068,
[channel 136 data]
068,
[channel 137 data]
068,
[channel 138 data]
068,
[channel 139 data]
078,
[channel 140 data]
080,
[channel 141 data]
080,
[channel 142 data]
080,
[channel 143 data]
080,
[channel 144 data]
073,
[channel 145 data]
073,
[channel 146 data]
073,
[channel 147 data]
073,
[channel 148 data]
073,
[channel 149 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
47
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HISTOGRAM MESSAGE EXAMPLE (Continued)
073,
[channel 150 data]
075,
[channel 151 data]
083,
[channel 152 data]
083,
[channel 153 data]
083,
[channel 154 data]
083,
[channel 155 data]
083,
[channel 156 data]
083,
[channel 157 data]
083,
[channel 158 data]
083,
[channel 159 data]
088,
[channel 160 data]
090,
[channel 161 data]
090,
[channel 162 data]
090,
[channel 163 data]
090,
[channel 164 data]
090,
[channel 165 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
48
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HISTOGRAM MESSAGE EXAMPLE (Continued)
090,
[channel 166 data]
090,
[channel 167 data]
090,
[channel 168 data]
090,
[channel 169 data]
095,
[channel 170 data]
095,
[channel 171 data]
095,
[channel 172 data]
095,
[channel 173 data]
110,
[channel 174 data]
095,
[channel 175 data]
095,
[channel 176 data]
095,
[channel 177 data]
110,
[channel 178 data]
110,
[channel 179 data]
110,
[channel 180 data]
110,
[channel 181 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
49
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HISTOGRAM MESSAGE EXAMPLE (Continued)
110,
[channel 182 data]
110,
[channel 183 data]
110,
[channel 184 data]
110,
[channel 185 data]
110,
[channel 186 data]
100,
[channel 187 data]
100,
[channel 188 data]
100,
[channel 189 data]
100,
[channel 190 data]
100,
[channel 191 data]
100,
[channel 192 data]
093,
[channel 193 data]
085,
[channel 194 data]
085,
[channel 195 data]
085,
[channel 196 data]
085,
[channel 197 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
50
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HISTOGRAM MESSAGE EXAMPLE (Continued)
085,
[channel 198 data]
078,
[channel 199 data]
078,
[channel 200 data]
085,
[channel 201 data]
085,
[channel 202 data]
085,
[channel 203 data]
085,
[channel 204 data]
085,
[channel 205 data]
085,
[channel 206 data]
085,
[channel 207 data]
083,
[channel 208 data]
083,
[channel 209 data]
083,
[channel 210 data]
083,
[channel 211 data]
073,
[channel 212 data]
083,
[channel 213 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
51
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HISTOGRAM MESSAGE EXAMPLE (Continued)
083,
[channel 214 data]
083,
[channel 215 data]
083,
[channel 216 data]
083,
[channel 217 data]
083,
[channel 218 data]
083,
[channel 219 data]
083,
[channel 220 data]
066,
[channel 221 data]
054,
[channel 222 data]
054,
[channel 223 data]
054,
[channel 224 data]
054,
[channel 225 data]
054,
[channel 226 data]
054,
[channel 227 data]
051,
[channel 228 data]
051,
[channel 229 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
52
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HISTOGRAM MESSAGE EXAMPLE (Continued)
051,
[channel 230 data]
051,
[channel 231 data]
051,
[channel 232 data]
051,
[channel 233 data]
051,
[channel 234 data]
046,
[channel 235 data]
034,
[channel 236 data]
034,
[channel 237 data]
034,
[channel 238 data]
034,
[channel 239 data]
034,
[channel 240 data]
034,
[channel 241 data]
034,
[channel 242 data]
034,
[channel 243 data]
034,
[channel 244 data]
034,
[channel 245 data]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
53
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HISTOGRAM MESSAGE EXAMPLE (Continued)
034,
[channel 246 data]
034,
[channel 247 data]
027,
[channel 248 data]
027,
[channel 249 data]
027,
[channel 250 data]
027,
[channel 251 data]
027,
[channel 252 data]
027,
[channel 253 data]
000,
[channel 254 data]
000,
[channel 255 data]
000,
[channel 256 data]
Field 273
[note comma after
channel 256 but not
after the checksum]
E7
[checksum]
NOTE: Sample checksum value may
not match this record's data.
[ETX
[end of text]
Interface Specification CELL-DYM® 1700 System
04H03-01A-February1995
54
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COUNT DATA MESSAGE EXAMPLE
[STX]
[start of text]
"WBC",
[message type]
"CD1700C",
[instrument type]
"------------",
[serial #]
1040,
[sequence no.]
00,
[specimen type]
"123",
[operator ID]
"08/31/94",
[specimen date]
"12:34",
[specimen time]
"123456789",
[specimen ID]
"
[specimen name]
JOHN DOE",
"M",
[specimen sex]
"10/1 5/46",
[specimen DOB
"
[doctor name]
DR JANE DOE",
"08/30",
[collect date]
'09:50",
[collect time]
Interface Specification CELL-DYM® 1700 System
Field 1
Field 5
Field 10
Field 15
04H03-01A-February1995
55
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COUNT DATA MESSAGE EXAMPLE (Continued)
"
sample 1",
[comment]
Field 16
00083,
[WBC count]
-----,
[spare field]
00015,
[LYM count]
00006,
[MID count]
00062,
[GRAN count]
-----,
[spare field]
00476,
[RBC count]
Field 23
00166,
[HGB value]
Field 24
00447,
[HCT value]
00940,
[MCV value]
00349,
[MCH value]
00371,
[MCHC value]
00118,
[RDW value]
00230,
[PLT value]
00084,
[MPV value]
Interface Specification CELL-DYM® 1700 System
Field 17
Field 20
Field 30
04H03-01A-February1995
56
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COUNT DATA MESSAGE EXAMPLE (Continued)
00019,
[PCT value]
00174,
[PDW value]
-----,
[spare field]
00178,
[LYM% value]
00077,
[MID% value]
00745,
[GRAN% value]
-----,
[spare field]
-,
[spare field]
-,
[spare field]
0,
[R4 WBC flag]
0,
[MR3 WBC flag]
1,
[MR2 WBC flag]
0,
[LR2 WBC flag}
0,
[R1 WBC flag]
1,
[RO WBC fiag]
-,
[spare field]
Interface Specification CELL-DYM® 1700 System
Field 35
Field 40
Field 45
Field 48
04H03-01A-February1995
57
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COUNT DATA MESSAGE EXAMPLE (Continued)
-,
[spare field]
-,
[spare field]
-,
[spare field]
-,
[spare field]
-,
[spare field]
-,
[spare field]
-,
[spare field]
-,
[spare field]
-,
[spare field]
-,
[spare field]
-,
[spare field]
-,
[spare field]
00,
[LRI flag]
00,
[URI flag]
04862,
[WBC lower meniscus time]
02363,
[WBC upper meniscus time]
Interface Specification CELL-DYM® 1700 System
Field 49
Field 50
Field 55
Field 60
04H03-01A-February1995
58
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COUNT DATA MESSAGE EXAMPLE (Continued)
08107,
[RBC lower meniscus time]
07656,
[RBC upper meniscus time]
00000,
[recount lower time]
21845,
[recount upper time]
1,
[limits set]
"C",
[sample mode]
0,
[RBC metering fault]
0,
[WBC metering fault]
0,
[sample error/incomplete
aspiration]
Field 73
1
[units set field]
Field 74
9B
[checksum]
NOTE: Sample checksum value
may not match this record's data.
[ETX]
[end of text]
Interface Specification CELL-DYM® 1700 System
Field 70
04H03-01A-February1995
59